Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0006465

Physical Location & Seq

Genomic Coordinates Forward (+)

12235866 .. 12242779

Gene Structure 6914 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1133400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2529 bp

ATGAGGATTTTAGTGGTGGTTTTGGTGATCGGTGTTCTGGCGACGGCGTCGTATTGCGCGAAGGTGACGTACGACCACAGAGCGCTGGTGATTGACGGGAAGCGCAGGGTTTTGGTGTCTGGCTCCATTCACTATCCTCGCAGCACTCCCGAGATGTGGCCGGACCTTATTCAGAAATCTAAAGACGGAGGCTTGGACGTGATCGAGACCTACGTCTTCTGGAACTTGCACGAACCAGTTCGAGGCCAGTATGATTTTGGGGGAAGAAAAGATTTGGTTAAATTTGTGAAGACAGTAGCAGAAGCTGGCCTTTATCTGCACCTTCGAATTGGTCCATATGTGTGCGCAGAATGGAACTACGGTGGCTTTCCTCTTTGGTTGCATTTCATACCCGGAATTCAATTGCGAACGGACAATGAACCATTCAAGGCAGAAATGCAGCGATTCACGGCAAAAATTGTTGATATGATGAAGAAGGAGAAGCTTTATGCATCTCAGGGCGGACCAATCATTTTATCTCAGATTGAAAACGAGTATGGAAACATTGATCAAGCATACGGGCCAGCTGCCCAAACCTATATTAAGTGGGCAGCAGCCATGGCTGTGTCTTTGGATACAGGGGTGCCTTGGGTCATGTGCCAGCAAGACGATGCTCCTCCATCTGTGATCAGCACTTGCAATGGATTTTATTGTGACCAATGGACCCCAAAACTTCCTGAAAAGAGACCCAAAATGTGGACTGAGAATTGGAGCGGATGGTTTCTTTCCTTTGGTGGTGCTGTGCCTCAGAGACCAGTGGAAGACCTTGCATTTGCCGTAGCACGCTTCTTTCAGCGAGGTGGAACTTTCCAAAATTATTATATGTACCATGGCGGGACTAACTTTGGGCGGAGTACTGGTGGACCCTTTATTGCTACAAGCTATGACTATGATGCTCCAATTGATGAGTATGGACTTCTTAGACAACCTAAGTGGGGTCACCTAAAAGATGTGCATAAGGCCATAAAGCTTTGCGAAGAAGCGATGGTGGCCACTGACCCAAAATATTCTTCTTTTGGTCCAAACGTTGAGACAACTGTTTACAAAACAGGATCAGCATGTGCTGCGTTTCTTGCTAATTCTGACACCAAATCTGATGCCACTGTGACTTTCAATGGAAATTCTTATCACTTGCCTGCATGGTCTGTGAGCATCTTACCAGACTGCAAAAATGTAGTGCTTAACACTGCAAAGATTAACTCTGCCGCCATGATTCCAAGCTTCATGCATCACTCAGTGATAGATGATATCGATTCCTCTGAAGCTTTGGGCTCAGGCTGGAGTTGGATAAATGAACCAGTGGGTATCTCAAAGAAGGATGCATTTACCAGAGTTGGATTGTTGGAGCAAATAAACACCACAGCTGACAAAAGTGATTATTTGTGGTACTCTTTAAGCGTTGATGTCACAAGTTCTGATACATTCCTTCAAGATGGATCTCAAACCGTTCTTCATGTGGAATCACTTGGCCATGCCCTTCACGTGTTTATTAACGGAAAGCCTGCAGGGAGAGGAATAATCACTTCTAAGAATGCCAAAATTTCTGTGGATATCCCTGTCACATTTGCATCTGGGAAGAACACAATAGATCTTCTGAGTTTGACTGTCGGCCTTCAGAACTATGGAGCCTTCTTTGACAAAGCAGGTGCCGGAATCACTGGTCCTGTGCAGCTGAAGGGCTTGAAAAATGGCACTACCACTGATCTCTCGTCTCAGCAATGGACCTATCAGATTGGACTTCAAGGGGAAGACTCGGGTTTTTCCAGCGGAAGTTCTTCTCAGTGGATTTCACAACCTACCTTACCCAAGAAACAGCCCTTGACATGGTACAAGGCAACATTTAGTGCTCCTGATGGCAGTAACCCTGTTGCACTAGACTTTACAGGGATGGGAAAGGGTGAAGCATGGGTGAACGGACAAAGCATTGGGCGGTACTGGCCGACCAACAATGCACCAACTAGCGGTTGCCCTGACTCTTGCAATTTCAGAGGACCTTATGACTCCAACAAATGCAGAAAGAACTGTGGAAAGCCATCTCAGGAATTGTACCATGTGCCTCGTTCATGGTTGAAACCAAGTGGCAATACTCTTGTCCTGTTTGAGGAAATTGGGGGTGATCCAACGCAGATATCTTTTGCTACAAGACAGATTGAAAGTTTGTGCTCACATGTATCGGAGTCTCATCCGTCACCTGTTGATACATGGAGTTCAGATTCAAAAGCGGGAAGGAAGTTAGGGCCTGTGCTATCACTAGAGTGCCCATTTCCTAACCAGGTCATTTCTTCAATCAAATTTGCAAGTTACGGAAAACCTCACGGGACCTGTGGGAGCTTTAGCCATGGCCACTGCAAGAGCACTAGCGCTCTATCCATTGTACAGAAGGCTTGCGTTGGATCCAAGAGTTGCAGCATTGAAGTCTCGGTTAAAACGTTCGGTGACCCGTGTGAAGGAGTAGCAAAGAGTTTAGCAGTAGAAGCATCCTGTAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

843

Amino Acids

92.02

Weight (kDa)

7.24

Isoelectric Point (pI)

37.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	29 - 334	1.5e-122	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	50 - 197	2.8e-06	Beta-galactosidase
GHD	PF17834	358 - 412	4.4e-24	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	620 - 708	6.3e-26	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	764 - 841	1.1e-20	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006465)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28470 AT2G28470 AT2G28470 AT2G28470 AT2G28470
fragaria_vesca	FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630
malus_domestica	MD03G1114300.v1.1 MD11G1133400.v1.1
prunus_persica	Prupe.6G099700_v2.0.a1 Prupe.6G099700_v2.0.a1 Prupe.6G099700_v2.0.a1
pyrus_communis	pycom11g11010
rosa_chinensis	RchiOBHm_Chr5g0059701
rosa_laevigata	RLG00000035321
rosa_multiflora	Rmu_sc0000660.1_g000045
rosa_roxburghii	Rroxscaffold_1G00020930
rosa_rugosa	Rorug05G0324200
rosa_samantha	Rh5AG389700 Rh5BG401300 Rh5DG417700
rosa_wichuraiana	Rw5G036670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1673
Acc16I	TGCGCA	1 cut(s)	346
Acc36I	ACCTGC	1 cut(s)	1673
AccB1I	GGYRCC	2 cut(s)	622, 1685
AccB7I	CCANNNNNTGG	1 cut(s)	735
AccBSI	CCGCTC	1 cut(s)	753
AccII	CGCG	1 cut(s)	59
AciI	CCGC	9 cut(s)	501, 753, 873, 889, 1245, 1806, 1969, 2001, 2261
AclI	AACGTT	2 cut(s)	1065, 2468
AclWI	GGATC	5 cut(s)	1099, 1483, 2150, 2427, 2440
AcoI	YGGCCR	5 cut(s)	158, 1029, 1507, 1976, 2380
AcsI	RAATTY	5 cut(s)	281, 396, 1159, 1578, 2330
AcuI	CTGAAG	3 cut(s)	1320, 1637, 1733
AcvI	CACGTG	1 cut(s)	1522
AcyI	GRCGYC	1 cut(s)	47
AdeI	CACNNNGTG	1 cut(s)	1276
AfaI	GTAC	8 cut(s)	71, 866, 895, 1427, 1868, 1973, 2087, 2415
AfeI	AGCGCT	2 cut(s)	84, 2401
AfiI	CCNNNNNNNGG	8 cut(s)	156, 242, 735, 974, 1863, 2000, 2140, 2486
AflIII	ACRYGT	2 cut(s)	1521, 2206
AjiI	CACGTC	1 cut(s)	199
AjnI	CCWGG	1 cut(s)	2310
AjuI	GAANNNNNNNTTGG	2 cut(s)	1033, 1065
AleI	CACNNNNGTG	1 cut(s)	2293
Alw21I	GWGCWC	3 cut(s)	1888, 2204, 2396
Alw26I	GTCTC	7 cut(s)	200, 718, 784, 1064, 1755, 2223, 2461
AlwI	GGATC	5 cut(s)	1099, 1483, 2150, 2427, 2440
AlwNI	CAGNNNCTG	1 cut(s)	305
Ama87I	CYCGRG	2 cut(s)	149, 1792
Aor51HI	AGCGCT	2 cut(s)	84, 2401
ApeKI	GCWGC	8 cut(s)	141, 439, 566, 590, 593, 1103, 1708, 2445
ApoI	RAATTY	5 cut(s)	281, 396, 1159, 1578, 2330
ArsI	GACNNNNNNTTYG	2 cut(s)	794, 826
Asp700I	GAANNNNTTC	2 cut(s)	237, 1813
AspLEI	GCGC	5 cut(s)	59, 85, 105, 347, 2402
AsuC2I	CCSGG	1 cut(s)	393
AsuHPI	GGTGA	9 cut(s)	37, 76, 100, 971, 1949, 1960, 2165, 2220, 2486
AsuII	TTCGAA	1 cut(s)	325
AvaI	CYCGRG	2 cut(s)	149, 1792
BaeGI	GKGCMC	1 cut(s)	2300
BaeI	ACNNNNGTAYC	2 cut(s)	606, 639
BalI	TGGCCA	3 cut(s)	1031, 1509, 2382
BamHI	GGATCC	1 cut(s)	2432
BanI	GGYRCC	2 cut(s)	622, 1685
BanII	GRGCYC	1 cut(s)	1313
BarI	GAAGNNNNNNTAC	2 cut(s)	53, 85
BbrPI	CACGTG	1 cut(s)	1522
BbsI	GAAGAC	4 cut(s)	208, 296, 807, 1794
Bbv12I	GWGCWC	3 cut(s)	1888, 2204, 2396
BbvI	GCAGC	8 cut(s)	153, 451, 553, 602, 605, 1090, 1720, 2457
BccI	CCATC	7 cut(s)	667, 750, 1018, 1466, 1886, 1921, 2080
BceAI	ACGGC	3 cut(s)	60, 465, 800
BciT130I	CCWGG	1 cut(s)	2312
BciVI	GTATCC	1 cut(s)	607
BclI	TGATCA	2 cut(s)	547, 666
BcnI	CCSGG	1 cut(s)	393
BcoDI	GTCTC	7 cut(s)	200, 718, 784, 1064, 1755, 2223, 2461
BfaI	CTAG	4 cut(s)	1913, 1998, 2291, 2397
BfmI	CTRYAG	2 cut(s)	1542, 2521
BfoI	RGCGCY	2 cut(s)	86, 2403
BfuAI	ACCTGC	1 cut(s)	1673
BfuI	GTATCC	1 cut(s)	607
BglII	AGATCT	1 cut(s)	1627
BisI	GCNGC	9 cut(s)	142, 440, 567, 591, 594, 1104, 1245, 1709, 2446
BlsI	GCNGC	9 cut(s)	143, 441, 568, 592, 595, 1105, 1246, 1710, 2447
BmcAI	AGTACT	1 cut(s)	895
Bme1390I	CCNGG	2 cut(s)	393, 2312
BmeT110I	CYCGRG	2 cut(s)	149, 1792
BmgBI	CACGTC	1 cut(s)	199
BmiI	GGNNCC	8 cut(s)	124, 624, 704, 904, 1666, 1687, 2359, 2434
BmrFI	CCNGG	2 cut(s)	393, 2312
BmsI	GCATC	9 cut(s)	500, 640, 922, 1126, 1200, 1276, 1348, 1616, 2525
BoxI	GACNNNNGTC	1 cut(s)	212
BpiI	GAAGAC	4 cut(s)	208, 296, 807, 1794
BpmI	CTGGAG	1 cut(s)	1339
Bpu10I	CCTNAGC	1 cut(s)	1312
Bpu14I	TTCGAA	1 cut(s)	325
BpuMI	CCSGG	1 cut(s)	393
Bsa29I	ATCGAT	1 cut(s)	1290
BsaAI	YACGTR	1 cut(s)	1522
BsaHI	GRCGYC	1 cut(s)	47
BsaI	GGTCTC	3 cut(s)	200, 718, 784
BsaJI	CCNNGG	4 cut(s)	597, 626, 868, 2377
BsaXI	ACNNNNNCTCC	2 cut(s)	883, 913
Bsc4I	CCNNNNNNNGG	8 cut(s)	156, 242, 735, 974, 1863, 2000, 2140, 2486
Bse1I	ACTGG	7 cut(s)	236, 247, 794, 901, 1337, 1702, 1979
Bse3DI	GCAATG	2 cut(s)	685, 1763
BseBI	CCWGG	1 cut(s)	2312
BseCI	ATCGAT	1 cut(s)	1290
BseDI	CCNNGG	4 cut(s)	597, 626, 868, 2377
BseGI	GGATG	5 cut(s)	761, 1363, 1932, 2221, 2516
BseLI	CCNNNNNNNGG	8 cut(s)	156, 242, 735, 974, 1863, 2000, 2140, 2486
BseMI	GCAATG	2 cut(s)	685, 1763
BseNI	ACTGG	7 cut(s)	236, 247, 794, 901, 1337, 1702, 1979
BseRI	GAGGAG	1 cut(s)	645
BseSI	GKGCMC	1 cut(s)	2300
BseXI	GCAGC	8 cut(s)	153, 451, 553, 602, 605, 1090, 1720, 2457
BsgI	GTGCAG	2 cut(s)	302, 1727
Bsh1236I	CGCG	1 cut(s)	59
BshNI	GGYRCC	2 cut(s)	622, 1685
BshVI	ATCGAT	1 cut(s)	1290
BsiHKAI	GWGCWC	3 cut(s)	1888, 2204, 2396
BsiHKCI	CYCGRG	2 cut(s)	149, 1792
BsiSI	CCGG	3 cut(s)	161, 393, 1689
BsiWI	CGTACG	1 cut(s)	69
BslFI	GGGAC	2 cut(s)	889, 2371
BslI	CCNNNNNNNGG	8 cut(s)	156, 242, 735, 974, 1863, 2000, 2140, 2486
BsmAI	GTCTC	7 cut(s)	200, 718, 784, 1064, 1755, 2223, 2461
BsmBI	CGTCTC	1 cut(s)	1755
BsmFI	GGGAC	2 cut(s)	889, 2371
BsmI	GAATGC	1 cut(s)	1576
Bso31I	GGTCTC	3 cut(s)	200, 718, 784
BsoBI	CYCGRG	2 cut(s)	149, 1792
Bsp119I	TTCGAA	1 cut(s)	325
Bsp1286I	GDGCHC	5 cut(s)	1313, 1888, 2204, 2300, 2396
Bsp1407I	TGTACA	1 cut(s)	2413
Bsp19I	CCATGG	3 cut(s)	597, 868, 2377
BspACI	CCGC	9 cut(s)	501, 753, 873, 889, 1245, 1806, 1969, 2001, 2261
BspDI	ATCGAT	1 cut(s)	1290
BspFNI	CGCG	1 cut(s)	59
BspLI	GGNNCC	8 cut(s)	124, 624, 704, 904, 1666, 1687, 2359, 2434
BspMAI	CTGCAG	1 cut(s)	1546
BspMI	ACCTGC	1 cut(s)	1673
BspPI	GGATC	5 cut(s)	1099, 1483, 2150, 2427, 2440
BspT104I	TTCGAA	1 cut(s)	325
BspT107I	GGYRCC	2 cut(s)	622, 1685
BspTNI	GGTCTC	3 cut(s)	200, 718, 784
BsrBI	CCGCTC	1 cut(s)	753
BsrDI	GCAATG	2 cut(s)	685, 1763
BsrGI	TGTACA	1 cut(s)	2413
BsrI	ACTGG	7 cut(s)	236, 247, 794, 901, 1337, 1702, 1979
BssECI	CCNNGG	4 cut(s)	597, 626, 868, 2377
BssNI	GRCGYC	1 cut(s)	47
BssT1I	CCWWGG	4 cut(s)	597, 626, 868, 2377
Bst2UI	CCWGG	1 cut(s)	2312
Bst4CI	ACNGT	7 cut(s)	295, 362, 1078, 1144, 1486, 1645, 2063
BstACI	GRCGYC	1 cut(s)	47
BstAPI	GCANNNNNTGC	1 cut(s)	1103
BstAUI	TGTACA	1 cut(s)	2413
BstBAI	YACGTR	1 cut(s)	1522
BstBI	TTCGAA	1 cut(s)	325
BstC8I	GCNNGC	7 cut(s)	307, 564, 641, 823, 1176, 1542, 2425
BstDSI	CCRYGG	3 cut(s)	597, 868, 2377
BstEII	GGTNACC	2 cut(s)	977, 2474
BstENI	CCTNNNNNAGG	1 cut(s)	2138
BstF5I	GGATG	5 cut(s)	761, 1363, 1932, 2221, 2516
BstFNI	CGCG	1 cut(s)	59
BstH2I	RGCGCY	2 cut(s)	86, 2403
BstHHI	GCGC	5 cut(s)	59, 85, 105, 347, 2402
BstMAI	GTCTC	7 cut(s)	200, 718, 784, 1064, 1755, 2223, 2461
BstMWI	GCNNNNNNNGC	6 cut(s)	599, 1028, 1103, 1112, 1975, 2513
BstNI	CCWGG	1 cut(s)	2312
BstNSI	RCATGY	2 cut(s)	1101, 2210
BstPAI	GACNNNNGTC	1 cut(s)	212
BstPI	GGTNACC	2 cut(s)	977, 2474
BstSCI	CCNGG	2 cut(s)	391, 2310
BstSFI	CTRYAG	2 cut(s)	1542, 2521
BstSLI	GKGCMC	1 cut(s)	2300
BstUI	CGCG	1 cut(s)	59
BstV1I	GCAGC	8 cut(s)	153, 451, 553, 602, 605, 1090, 1720, 2457
BstV2I	GAAGAC	4 cut(s)	208, 296, 807, 1794
BstX2I	RGATCY	3 cut(s)	1475, 1627, 2432
BstYI	RGATCY	3 cut(s)	1475, 1627, 2432
Bsu15I	ATCGAT	1 cut(s)	1290
BsuI	GTATCC	1 cut(s)	607
BsuTUI	ATCGAT	1 cut(s)	1290
BtgI	CCRYGG	3 cut(s)	597, 868, 2377
BtgZI	GCGATG	1 cut(s)	1037
BtrI	CACGTC	1 cut(s)	199
BtsCI	GGATG	5 cut(s)	761, 1363, 1932, 2221, 2516
BtsI	GCAGTG	2 cut(s)	1224, 2383
BveI	ACCTGC	1 cut(s)	1673
Cac8I	GCNNGC	7 cut(s)	307, 564, 641, 823, 1176, 1542, 2425
CaiI	CAGNNNCTG	1 cut(s)	305
CfoI	GCGC	5 cut(s)	59, 85, 105, 347, 2402
ClaI	ATCGAT	1 cut(s)	1290
CseI	GACGC	1 cut(s)	36
CsiI	ACCWGGT	1 cut(s)	2310
Csp6I	GTAC	8 cut(s)	70, 865, 894, 1426, 1867, 1972, 2086, 2414
CspCI	CAANNNNNGTGG	2 cut(s)	953, 988
CviQI	GTAC	8 cut(s)	70, 865, 894, 1426, 1867, 1972, 2086, 2414
DraIII	CACNNNGTG	1 cut(s)	1276
EaeI	YGGCCR	5 cut(s)	158, 1029, 1507, 1976, 2380
EciI	GGCGGA	2 cut(s)	516, 904
Eco130I	CCWWGG	4 cut(s)	597, 626, 868, 2377
Eco24I	GRGCYC	1 cut(s)	1313
Eco31I	GGTCTC	3 cut(s)	200, 718, 784
Eco32I	GATATC	3 cut(s)	1288, 1591, 2169
Eco47III	AGCGCT	2 cut(s)	84, 2401
Eco57I	CTGAAG	3 cut(s)	1320, 1637, 1733
Eco72I	CACGTG	1 cut(s)	1522
Eco88I	CYCGRG	2 cut(s)	149, 1792
Eco91I	GGTNACC	2 cut(s)	977, 2474
EcoNI	CCTNNNNNAGG	1 cut(s)	2138
EcoO109I	RGGNCCY	3 cut(s)	2030, 2276, 2358
EcoO65I	GGTNACC	2 cut(s)	977, 2474
EcoRI	GAATTC	1 cut(s)	396
EcoRII	CCWGG	1 cut(s)	2310
EcoRV	GATATC	3 cut(s)	1288, 1591, 2169
EcoT14I	CCWWGG	4 cut(s)	597, 626, 868, 2377
EcoT22I	ATGCAT	3 cut(s)	493, 1269, 1363
EcoT38I	GRGCYC	1 cut(s)	1313
ErhI	CCWWGG	4 cut(s)	597, 626, 868, 2377
Esp3I	CGTCTC	1 cut(s)	1755
FalI	AAGNNNNNCTT	2 cut(s)	294, 326
FaqI	GGGAC	2 cut(s)	889, 2371
FauI	CCCGC	2 cut(s)	866, 2254
FauNDI	CATATG	1 cut(s)	337
FbaI	TGATCA	2 cut(s)	547, 666
Fnu4HI	GCNGC	9 cut(s)	142, 440, 567, 591, 594, 1104, 1245, 1709, 2446
FokI	GGATG	5 cut(s)	768, 1370, 1939, 2208, 2503
FriOI	GRGCYC	1 cut(s)	1313
Fsp4HI	GCNGC	9 cut(s)	142, 440, 567, 591, 594, 1104, 1245, 1709, 2446
FspBI	CTAG	4 cut(s)	1913, 1998, 2291, 2397
FspI	TGCGCA	1 cut(s)	346
GlaI	GCGC	5 cut(s)	58, 84, 104, 346, 2401
GluI	GCNGC	9 cut(s)	142, 440, 567, 591, 594, 1104, 1245, 1709, 2446
GsuI	CTGGAG	1 cut(s)	1339
HaeII	RGCGCY	2 cut(s)	86, 2403
HapII	CCGG	3 cut(s)	161, 393, 1689
HgaI	GACGC	1 cut(s)	36
HhaI	GCGC	5 cut(s)	59, 85, 105, 347, 2402
Hin1I	GRCGYC	1 cut(s)	47
Hin6I	GCGC	5 cut(s)	57, 83, 103, 345, 2400
HinP1I	GCGC	5 cut(s)	57, 83, 103, 345, 2400
HindIII	AAGCTT	4 cut(s)	482, 1007, 1258, 1302
HpaII	CCGG	3 cut(s)	161, 393, 1689
HphI	GGTGA	9 cut(s)	37, 76, 100, 971, 1949, 1960, 2165, 2220, 2486
Hpy166II	GTNNAC	4 cut(s)	738, 902, 1081, 1951
Hpy188III	TCNNGA	7 cut(s)	149, 205, 220, 716, 1469, 1889, 2078
Hpy8I	GTNNAC	4 cut(s)	738, 902, 1081, 1951
Hpy99I	CGWCG	2 cut(s)	46, 52
HpyCH4III	ACNGT	7 cut(s)	295, 362, 1078, 1144, 1486, 1645, 2063
HpyCH4IV	ACGT	6 cut(s)	68, 198, 213, 1065, 1521, 2468
HpyF10VI	GCNNNNNNNGC	6 cut(s)	599, 1028, 1103, 1112, 1975, 2513
HpySE526I	ACGT	6 cut(s)	68, 198, 213, 1065, 1521, 2468
Hsp92I	GRCGYC	1 cut(s)	47
HspAI	GCGC	5 cut(s)	57, 83, 103, 345, 2400
Ksp22I	TGATCA	2 cut(s)	547, 666
LmnI	GCTCC	8 cut(s)	128, 658, 750, 940, 1384, 1664, 1891, 2367
Lsp1109I	GCAGC	8 cut(s)	153, 451, 553, 602, 605, 1090, 1720, 2457
LweI	GCATC	9 cut(s)	500, 640, 922, 1126, 1200, 1276, 1348, 1616, 2525
MabI	ACCWGGT	1 cut(s)	2310
MaeI	CTAG	4 cut(s)	1913, 1998, 2291, 2397
MaeII	ACGT	6 cut(s)	68, 198, 213, 1065, 1521, 2468
MbiI	CCGCTC	1 cut(s)	753
MfeI	CAATTG	2 cut(s)	401, 939
MflI	RGATCY	3 cut(s)	1475, 1627, 2432
MhlI	GDGCHC	5 cut(s)	1313, 1888, 2204, 2300, 2396
MlsI	TGGCCA	3 cut(s)	1031, 1509, 2382
MluNI	TGGCCA	3 cut(s)	1031, 1509, 2382
MlyI	GAGTC	4 cut(s)	1784, 2006, 2033, 2225
MmeI	TCCRAC	6 cut(s)	1304, 1354, 1362, 2067, 2183, 2410
Mox20I	TGGCCA	3 cut(s)	1031, 1509, 2382
Mph1103I	ATGCAT	3 cut(s)	493, 1269, 1363
MroXI	GAANNNNTTC	2 cut(s)	237, 1813
MscI	TGGCCA	3 cut(s)	1031, 1509, 2382
MseI	TTAA	7 cut(s)	279, 582, 1221, 1236, 1433, 1530, 2463
MslI	CAYNNNNRTG	3 cut(s)	340, 602, 2293
Msp20I	TGGCCA	3 cut(s)	1031, 1509, 2382
MspA1I	CMGCKG	4 cut(s)	566, 1403, 1711, 1806
MspI	CCGG	3 cut(s)	161, 393, 1689
MspR9I	CCNGG	2 cut(s)	393, 2312
MunI	CAATTG	2 cut(s)	401, 939
Mva1269I	GAATGC	1 cut(s)	1576
MvaI	CCWGG	1 cut(s)	2312
MvnI	CGCG	1 cut(s)	59
MwoI	GCNNNNNNNGC	6 cut(s)	599, 1028, 1103, 1112, 1975, 2513
NciI	CCSGG	1 cut(s)	393
NcoI	CCATGG	3 cut(s)	597, 868, 2377
NdeI	CATATG	1 cut(s)	337
NlaIV	GGNNCC	8 cut(s)	124, 624, 704, 904, 1666, 1687, 2359, 2434
NmuCI	GTSAC	8 cut(s)	64, 692, 977, 1144, 1444, 1597, 2226, 2474
NsbI	TGCGCA	1 cut(s)	346
NsiI	ATGCAT	3 cut(s)	493, 1269, 1363
NspI	RCATGY	2 cut(s)	1101, 2210
NspV	TTCGAA	1 cut(s)	325
OliI	CACNNNNGTG	1 cut(s)	2293
PaqCI	CACCTGC	1 cut(s)	1673
PciI	ACATGT	1 cut(s)	2206
PcsI	WCGNNNNNNNCGW	3 cut(s)	56, 210, 2465
PctI	GAATGC	1 cut(s)	1576
PdmI	GAANNNNTTC	2 cut(s)	237, 1813
PfeI	GAWTC	6 cut(s)	444, 1252, 1292, 1499, 1692, 2252
Pfl23II	CGTACG	1 cut(s)	69
PflFI	GACNNNGTC	1 cut(s)	46
PflMI	CCANNNNNTGG	1 cut(s)	735
PkrI	GCNGC	9 cut(s)	143, 441, 568, 592, 595, 1105, 1246, 1710, 2447
PleI	GAGTC	4 cut(s)	1784, 2006, 2033, 2224
PmaCI	CACGTG	1 cut(s)	1522
PmlI	CACGTG	1 cut(s)	1522
PpsI	GAGTC	4 cut(s)	1784, 2006, 2033, 2224
Ppu21I	YACGTR	1 cut(s)	1522
PpuMI	RGGWCCY	2 cut(s)	2030, 2358
PscI	ACATGT	1 cut(s)	2206
PshAI	GACNNNNGTC	1 cut(s)	212
Psp1406I	AACGTT	2 cut(s)	1065, 2468
Psp5II	RGGWCCY	2 cut(s)	2030, 2358
Psp6I	CCWGG	1 cut(s)	2310
PspCI	CACGTG	1 cut(s)	1522
PspEI	GGTNACC	2 cut(s)	977, 2474
PspGI	CCWGG	1 cut(s)	2310
PspLI	CGTACG	1 cut(s)	69
PspN4I	GGNNCC	8 cut(s)	124, 624, 704, 904, 1666, 1687, 2359, 2434
PspPPI	RGGWCCY	2 cut(s)	2030, 2358
PstI	CTGCAG	1 cut(s)	1546
PstNI	CAGNNNCTG	1 cut(s)	305
PsuI	RGATCY	3 cut(s)	1475, 1627, 2432
PsyI	GACNNNGTC	1 cut(s)	46
PvuII	CAGCTG	3 cut(s)	566, 1403, 1711
RsaI	GTAC	8 cut(s)	71, 866, 895, 1427, 1868, 1973, 2087, 2415
RsaNI	GTAC	8 cut(s)	70, 865, 894, 1426, 1867, 1972, 2086, 2414
RseI	CAYNNNNRTG	3 cut(s)	340, 602, 2293
SaqAI	TTAA	7 cut(s)	279, 582, 1221, 1236, 1433, 1530, 2463
SatI	GCNGC	9 cut(s)	142, 440, 567, 591, 594, 1104, 1245, 1709, 2446
SbfI	CCTGCAGG	1 cut(s)	1546
ScaI	AGTACT	1 cut(s)	895
SchI	GAGTC	4 cut(s)	1784, 2006, 2033, 2225
ScrFI	CCNGG	2 cut(s)	393, 2312
SdaI	CCTGCAGG	1 cut(s)	1546
SduI	GDGCHC	5 cut(s)	1313, 1888, 2204, 2300, 2396
SexAI	ACCWGGT	1 cut(s)	2310
SfaNI	GCATC	9 cut(s)	500, 640, 922, 1126, 1200, 1276, 1348, 1616, 2525
SfcI	CTRYAG	2 cut(s)	1542, 2521
SfuI	TTCGAA	1 cut(s)	325
SmiMI	CAYNNNNRTG	3 cut(s)	340, 602, 2293
Sse8387I	CCTGCAGG	1 cut(s)	1546
SsiI	CCGC	9 cut(s)	501, 753, 873, 889, 1245, 1806, 1969, 2001, 2261
SspI	AATATT	1 cut(s)	1046
SspMI	CTAG	4 cut(s)	1913, 1998, 2291, 2397
StyD4I	CCNGG	2 cut(s)	391, 2310
StyI	CCWWGG	4 cut(s)	597, 626, 868, 2377
TaaI	ACNGT	7 cut(s)	295, 362, 1078, 1144, 1486, 1645, 2063
TaiI	ACGT	6 cut(s)	71, 201, 216, 1068, 1524, 2471
TaqI	TCGA	4 cut(s)	204, 241, 325, 1290
TatI	WGTACW	2 cut(s)	893, 2413
TauI	GCSGC	1 cut(s)	1247
TfiI	GAWTC	6 cut(s)	444, 1252, 1292, 1499, 1692, 2252
Tru1I	TTAA	7 cut(s)	279, 582, 1221, 1236, 1433, 1530, 2463
Tru9I	TTAA	7 cut(s)	279, 582, 1221, 1236, 1433, 1530, 2463
TseFI	GTSAC	8 cut(s)	64, 692, 977, 1144, 1444, 1597, 2226, 2474
TseI	GCWGC	8 cut(s)	141, 439, 566, 590, 593, 1103, 1708, 2445
Tsp45I	GTSAC	8 cut(s)	64, 692, 977, 1144, 1444, 1597, 2226, 2474
TspDTI	ATGAA	7 cut(s)	376, 432, 485, 1252, 1347, 1481, 2091
TspGWI	ACGGA	6 cut(s)	201, 425, 1548, 1968, 2214, 2358
Tth111I	GACNNNGTC	1 cut(s)	46
Van91I	CCANNNNNTGG	1 cut(s)	735
XagI	CCTNNNNNAGG	1 cut(s)	2138
XapI	RAATTY	5 cut(s)	281, 396, 1159, 1578, 2330
XceI	RCATGY	2 cut(s)	1101, 2210
XcmI	CCANNNNNNNNNTGG	2 cut(s)	254, 1582
XmnI	GAANNNNTTC	2 cut(s)	237, 1813
XspI	CTAG	4 cut(s)	1913, 1998, 2291, 2397
ZrmI	AGTACT	1 cut(s)	895
Zsp2I	ATGCAT	3 cut(s)	493, 1269, 1363

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.