Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0006465

Physical Location & Seq

Genomic Coordinates Reverse (-)

27931773 .. 27939963

Gene Structure 8191 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g32630.t6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2523 bp

ATGGCTGTGGCAATGAGAGGAGTTGAGTTTAAGCTAGTGGTGCTTTTGGTGGTGGGAGTCTTGGCAACGGCGTCGTATTGCACGACGGTTAGTTACGACCACAGGGCGCTTGTGATCGATGGAAAGCGCAGAGTGTTAGTCTCTGGCTCGATTCACTACCCTCGTAGCACTCCAGAGATGTGGCCGGACCTTATTCAGAAATCCAAGGACGGAGGGTTGGATGTGATCGAGACCTATGTGTTTTGGAACTTACATGAGCCAGTTCGAGGCCAGTACAACTTTGAGGGAAGGAACGATTTGGTTGGATTTGTGAAGGCAGTGGCAGAAGCAGGGCTCTATGTGCATCTTCGAATTGGGCCTTATGTGTGCGCAGAATGGAATTACGGTGGCTTTCCTCTGTGGCTGCACTTCATACCCGGAATAAAGTTGCGGACTGATAATGAGCCCTACAAGGCAGAAATGCAGCGGTTCACGGCCAAAATCGTTGAAATGATGAAGAATGAGAAGCTTTATGCATCTCAGGGAGGACCTATCATTCTATCTCAGATCGAAAACGAGTATGGAAACATTGATAAAGCATATGGGCCTGCTGCCAAAACCTACATTAATTGGGCAGCAAACATGGCTGTGTCTTTGGATACAGGGGTGCCCTGGGTCATGTGCCAGCAAGCAGATGCTCCTTCTTCAGTGATCAACACTTGCAATGGATTTTACTGCGATCAATTTTCCCCTAATTCTAACAGTACACCCAAAATTTGGACTGAGAATTGGAGTGGATGGTTTCTTTCCTTTGGTGGTGCTGTACCTCAGAGACTGGTGGAAGACCTTGCATTTGCTGTAGCACGCTTCTACCAGCGAGGTGGAACTTTCCAAAATTATTATATGTACCATGGTGGAACCAACTTTGGGCGGTCTAGTGGAGGACCTTTTATTGCTACAAGTTATGACTATGATGCTCCACTTGATGAGTATGGACTTCTTAGGCAGCCGAAATGGGGTCATCTAAAAGATGTGCATAAGGCTATAAAGCTTTGTGAACCTGCAATGGTGGCCACTGATCCAACAATTTCTTCTCTCGGTCAAAACATTGAGGCAGCTGTTTACAAGACAGGATCAGTATGTTCTGCATTTCTTGCCAATGTCGACACCAAATCTGATGCCACTGTGACTTTCAATGGCAATTCATATCAATTGCCTGCATGGTCTGTGAGCATCTTACCTGACTGCAAAAATGTAGTGATTAACACTGCAAAGATTAACACTGCCACTATGGTTCCAAGCTTCACACGTCAATCAATTTCAGCTGATGTTGAGCCCACTGAAGCTGTCGGCTCAGGCTGGAGTTGGATAAATGAACCAGTGGGTATCTCAAAGGGTGATGCATTTACCAGAGTGGGATTATTGGAGCAGATAAACACTACAGCTGATAAAAGTGACTATTTGTGGTACTCTACCAGCATTGATGTGAAAGGTGGATATAAAGCTGATCTTCATGTGCAATCGCTTGGTCATGCTCTTCATGCTTTTGTCAATGGAAAGCTTGCAGGGAGTGAAACAGGCAACAGTGGCAATGCCAAGGTTTCTGTGGAGATCCCTGTCGAATTTGCATCTGGCAAGAACACAATTGATCTTTTGAGTCTGACCGTTGGGCTTCAGAATTATGGAGCTTTCTTTGACTTAGTGGGTGCTGGGATCACCGGTCCTGTACAACTGAAAGGCTCAGCAAATGGCACCACTATTGATCTCTCTTCTCAGCAATGGACATATCAGATTGGACTTAAAGGTGAAGACGAAGATCTTCCGAGTGGGAGTTCTCAATGGATCTCACAACCTACCTTGCCCAAGAATCAGCCCTTGACGTGGTACAAGACCCAATTTGATGCCCCTGGTGGCAGTAACCCTGTTGCACTAGACTTCACAGGAATGGGGAAGGGTGAGGCATGGGTGAATGGACAAAGCATTGGACGTTACTGGCCCACTAATGTTGCTCCGAAAACTGGTTGCACTGATTGCAACTACAGAGGAGCTTATAGTGCAGACAAATGCCGAAAGAACTGTGGCATGCCATCTCAGAAATTATACCACGTTCCTCGTTCATGGATGAAATCCAGTGGCAATACTCTTGTATTATTTGAGGAAGTTGGGGGTGATCCGACACAGTTATCTTTTGCCACAAGACAGGTAGAAAGTTTGTGCTCTCATGTATCAGAGTCTCACCCATCACCTGTAGATATGTGGAGCTCAGATTCCAAAGCAGGAAGCAAGTCACGGCCTAGGCTATCACTTGAGTGCCCATTTCCTAATCAAGTCATTTCTTCAATCAAGTTTGCAAGTTACGGAAGGCCTAGTGGGACTTGCGGGAGCTTTAGCCATGGCTTGTGTAGGAGCAGTAGGGCTCTATCCATTGTACAGAAGGCATGCGTTGGATCAAAGAGTTGTAGCATCGAAGTCTCAACTAATACATTTGGTGACCCATGTAAAGGACTAGCAAAGAGTTTAGCAGTAGAAGCTTCTTGTAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

841

Amino Acids

91.32

Weight (kDa)

6.93

Isoelectric Point (pI)

34.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	37 - 341	3.2e-123	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	58 - 205	7.5e-07	Beta-galactosidase
GHD	PF17834	365 - 419	1.3e-23	Beta-sandwich domain in beta galactosidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006465)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28470 AT2G28470 AT2G28470 AT2G28470 AT2G28470
fragaria_vesca	FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630
malus_domestica	MD03G1114300.v1.1 MD11G1133400.v1.1
prunus_persica	Prupe.6G099700_v2.0.a1 Prupe.6G099700_v2.0.a1 Prupe.6G099700_v2.0.a1
pyrus_communis	pycom11g11010
rosa_chinensis	RchiOBHm_Chr5g0059701
rosa_laevigata	RLG00000035321
rosa_multiflora	Rmu_sc0000660.1_g000045
rosa_roxburghii	Rroxscaffold_1G00020930
rosa_rugosa	Rorug05G0324200
rosa_samantha	Rh5AG389700 Rh5BG401300 Rh5DG417700
rosa_wichuraiana	Rw5G036670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	370
Acc36I	ACCTGC	1 cut(s)	1048
AccB1I	GGYRCC	2 cut(s)	646, 1730
AccB7I	CCANNNNNTGG	1 cut(s)	756
AccI	GTMKAC	1 cut(s)	1143
AciI	CCGC	4 cut(s)	430, 466, 910, 2358
AclWI	GGATC	7 cut(s)	1052, 1120, 1585, 1700, 1829, 2144, 2434
AcoI	YGGCCR	3 cut(s)	182, 474, 1050
AcsI	RAATTY	2 cut(s)	753, 1601
AcuI	CTGAAG	3 cut(s)	669, 1341, 1637
AcyI	GRCGYC	1 cut(s)	71
AfaI	GTAC	8 cut(s)	275, 745, 804, 887, 1448, 1707, 1865, 2409
AfiI	CCNNNNNNNGG	7 cut(s)	266, 756, 906, 995, 1860, 1997, 2480
AflIII	ACRYGT	1 cut(s)	1286
AgeI	ACCGGT	1 cut(s)	1697
AgsI	TTSAA	3 cut(s)	488, 1174, 2319
AjiI	CACGTC	2 cut(s)	1289, 1860
AjnI	CCWGG	2 cut(s)	650, 1885
AjuI	GAANNNNNNNTTGG	2 cut(s)	1054, 1086
AleI	CACNNNNGTG	1 cut(s)	2287
Alw21I	GWGCWC	2 cut(s)	2198, 2243
Alw26I	GTCTC	5 cut(s)	145, 224, 805, 2217, 2455
AlwI	GGATC	7 cut(s)	1052, 1120, 1585, 1700, 1829, 2144, 2434
AlwNI	CAGNNNCTG	1 cut(s)	814
AoxI	GGCC	9 cut(s)	182, 268, 356, 474, 584, 1050, 1973, 2270, 2342
ApeKI	GCWGC	6 cut(s)	403, 463, 590, 614, 985, 1094
ApoI	RAATTY	2 cut(s)	753, 1601
ArsI	GACNNNNNNTTYG	2 cut(s)	815, 847
AseI	ATTAAT	1 cut(s)	606
AsiGI	ACCGGT	1 cut(s)	1697
Asp700I	GAANNNNTTC	1 cut(s)	1797
AspA2I	CCTAGG	1 cut(s)	2273
AspLEI	GCGC	3 cut(s)	109, 129, 371
AspS9I	GGNCC	7 cut(s)	187, 356, 527, 584, 923, 1700, 1974
AsuC2I	CCSGG	1 cut(s)	417
AsuHPI	GGTGA	9 cut(s)	1388, 1687, 1796, 1946, 1957, 2159, 2207, 2214, 2480
AsuII	TTCGAA	1 cut(s)	349
AvaII	GGWCC	4 cut(s)	187, 527, 923, 1700
AvrII	CCTAGG	1 cut(s)	2273
BaeGI	GKGCMC	2 cut(s)	651, 2294
BaeI	ACNNNNGTAYC	2 cut(s)	630, 663
BalI	TGGCCA	1 cut(s)	1052
BanI	GGYRCC	2 cut(s)	646, 1730
BanII	GRGCYC	5 cut(s)	336, 447, 1317, 2243, 2398
BbsI	GAAGAC	2 cut(s)	828, 1794
Bbv12I	GWGCWC	2 cut(s)	2198, 2243
BbvI	GCAGC	6 cut(s)	390, 475, 577, 626, 997, 1106
BccI	CCATC	4 cut(s)	113, 771, 2074, 2227
BceAI	ACGGC	3 cut(s)	84, 489, 2285
BcgI	CGANNNNNNTGC	2 cut(s)	54, 88
BciT130I	CCWGG	2 cut(s)	652, 1887
BciVI	GTATCC	1 cut(s)	631
BclI	TGATCA	1 cut(s)	690
BcnI	CCSGG	1 cut(s)	417
BcoDI	GTCTC	5 cut(s)	145, 224, 805, 2217, 2455
BfaI	CTAG	6 cut(s)	35, 915, 1910, 2274, 2346, 2486
BfmI	CTRYAG	4 cut(s)	837, 1419, 2017, 2226
BfoI	RGCGCY	1 cut(s)	110
BfuAI	ACCTGC	1 cut(s)	1048
BfuI	GTATCC	1 cut(s)	631
BglII	AGATCT	1 cut(s)	1795
BisI	GCNGC	6 cut(s)	404, 464, 591, 615, 986, 1095
BlnI	CCTAGG	1 cut(s)	2273
BlpI	GCTNAGC	1 cut(s)	1720
BlsI	GCNGC	6 cut(s)	405, 465, 592, 616, 987, 1096
Bme1390I	CCNGG	3 cut(s)	417, 652, 1887
Bme18I	GGWCC	4 cut(s)	187, 527, 923, 1700
BmgBI	CACGTC	2 cut(s)	1289, 1860
BmgT120I	GGNCC	7 cut(s)	187, 356, 527, 584, 923, 1700, 1974
BmiI	GGNNCC	4 cut(s)	648, 898, 1275, 1732
BmrFI	CCNGG	3 cut(s)	417, 652, 1887
BpiI	GAAGAC	2 cut(s)	828, 1794
BpmI	CTGGAG	2 cut(s)	156, 1360
Bpu10I	CCTNAGC	1 cut(s)	1333
Bpu1102I	GCTNAGC	1 cut(s)	1720
Bpu14I	TTCGAA	1 cut(s)	349
BpuEI	CTTGAG	1 cut(s)	2306
BpuMI	CCSGG	1 cut(s)	417
Bsa29I	ATCGAT	1 cut(s)	117
BsaHI	GRCGYC	1 cut(s)	71
BsaI	GGTCTC	1 cut(s)	224
BsaJI	CCNNGG	8 cut(s)	204, 650, 651, 889, 1575, 1885, 2273, 2371
BsaWI	WCCGGW	1 cut(s)	1697
Bsc4I	CCNNNNNNNGG	7 cut(s)	266, 756, 906, 995, 1860, 1997, 2480
Bse118I	RCCGGY	1 cut(s)	1697
Bse1I	ACTGG	7 cut(s)	260, 271, 819, 1358, 1976, 2002, 2109
Bse3DI	GCAATG	5 cut(s)	18, 709, 1050, 1576, 1763
BseBI	CCWGG	2 cut(s)	652, 1887
BseCI	ATCGAT	1 cut(s)	117
BseDI	CCNNGG	8 cut(s)	204, 650, 651, 889, 1575, 1885, 2273, 2371
BseGI	GGATG	3 cut(s)	226, 782, 2106
BseLI	CCNNNNNNNGG	7 cut(s)	266, 756, 906, 995, 1860, 1997, 2480
BseMI	GCAATG	5 cut(s)	18, 709, 1050, 1576, 1763
BseMII	CTCAG	9 cut(s)	533, 557, 753, 821, 1347, 1734, 1766, 2084, 2256
BseNI	ACTGG	7 cut(s)	260, 271, 819, 1358, 1976, 2002, 2109
BseRI	GAGGAG	2 cut(s)	33, 2037
BseSI	GKGCMC	2 cut(s)	651, 2294
BseXI	GCAGC	6 cut(s)	390, 475, 577, 626, 997, 1106
BseYI	CCCAGC	1 cut(s)	1688
BsgI	GTGCAG	2 cut(s)	389, 2055
BshFI	GGCC	9 cut(s)	184, 270, 358, 476, 586, 1052, 1975, 2272, 2344
BshNI	GGYRCC	2 cut(s)	646, 1730
BshTI	ACCGGT	1 cut(s)	1697
BshVI	ATCGAT	1 cut(s)	117
BsiHKAI	GWGCWC	2 cut(s)	2198, 2243
BsiSI	CCGG	3 cut(s)	185, 417, 1698
BslFI	GGGAC	1 cut(s)	2365
BslI	CCNNNNNNNGG	7 cut(s)	266, 756, 906, 995, 1860, 1997, 2480
BsmAI	GTCTC	5 cut(s)	145, 224, 805, 2217, 2455
BsmFI	GGGAC	1 cut(s)	2365
BsnI	GGCC	9 cut(s)	184, 270, 358, 476, 586, 1052, 1975, 2272, 2344
Bso31I	GGTCTC	1 cut(s)	224
Bsp119I	TTCGAA	1 cut(s)	349
Bsp1286I	GDGCHC	8 cut(s)	336, 447, 651, 1317, 2198, 2243, 2294, 2398
Bsp1407I	TGTACA	2 cut(s)	1705, 2407
Bsp1720I	GCTNAGC	1 cut(s)	1720
Bsp19I	CCATGG	2 cut(s)	889, 2371
BspACI	CCGC	4 cut(s)	430, 466, 910, 2358
BspANI	GGCC	9 cut(s)	184, 270, 358, 476, 586, 1052, 1975, 2272, 2344
BspCNI	CTCAG	9 cut(s)	532, 556, 754, 820, 1346, 1733, 1765, 2083, 2255
BspDI	ATCGAT	1 cut(s)	117
BspLI	GGNNCC	4 cut(s)	648, 898, 1275, 1732
BspMI	ACCTGC	1 cut(s)	1048
BspPI	GGATC	7 cut(s)	1052, 1120, 1585, 1700, 1829, 2144, 2434
BspQI	GCTCTTC	1 cut(s)	1521
BspT104I	TTCGAA	1 cut(s)	349
BspT107I	GGYRCC	2 cut(s)	646, 1730
BspTNI	GGTCTC	1 cut(s)	224
BsrDI	GCAATG	5 cut(s)	18, 709, 1050, 1576, 1763
BsrFI	RCCGGY	1 cut(s)	1697
BsrGI	TGTACA	2 cut(s)	1705, 2407
BsrI	ACTGG	7 cut(s)	260, 271, 819, 1358, 1976, 2002, 2109
BssAI	RCCGGY	1 cut(s)	1697
BssECI	CCNNGG	8 cut(s)	204, 650, 651, 889, 1575, 1885, 2273, 2371
BssNI	GRCGYC	1 cut(s)	71
BssT1I	CCWWGG	5 cut(s)	204, 889, 1575, 2273, 2371
Bst2UI	CCWGG	2 cut(s)	652, 1887
Bst4CI	ACNGT	8 cut(s)	88, 386, 743, 1165, 1565, 1645, 2057, 2160
Bst6I	CTCTTC	2 cut(s)	1521, 1753
BstACI	GRCGYC	1 cut(s)	71
BstAPI	GCANNNNNTGC	2 cut(s)	1133, 2010
BstAUI	TGTACA	2 cut(s)	1705, 2407
BstBI	TTCGAA	1 cut(s)	349
BstC8I	GCNNGC	8 cut(s)	588, 665, 669, 844, 1197, 1542, 2063, 2419
BstDSI	CCRYGG	2 cut(s)	889, 2371
BstEII	GGTNACC	1 cut(s)	2468
BstF5I	GGATG	3 cut(s)	226, 782, 2106
BstH2I	RGCGCY	1 cut(s)	110
BstHHI	GCGC	3 cut(s)	109, 129, 371
BstMAI	GTCTC	5 cut(s)	145, 224, 805, 2217, 2455
BstNI	CCWGG	2 cut(s)	652, 1887
BstNSI	RCATGY	2 cut(s)	2065, 2421
BstPI	GGTNACC	1 cut(s)	2468
BstSCI	CCNGG	3 cut(s)	415, 650, 1885
BstSFI	CTRYAG	4 cut(s)	837, 1419, 2017, 2226
BstSLI	GKGCMC	2 cut(s)	651, 2294
BstV1I	GCAGC	6 cut(s)	390, 475, 577, 626, 997, 1106
BstV2I	GAAGAC	2 cut(s)	828, 1794
BstX2I	RGATCY	3 cut(s)	1590, 1795, 1821
BstXI	CCANNNNNNTGG	2 cut(s)	180, 860
BstYI	RGATCY	3 cut(s)	1590, 1795, 1821
Bsu15I	ATCGAT	1 cut(s)	117
BsuI	GTATCC	1 cut(s)	631
BsuRI	GGCC	9 cut(s)	184, 270, 358, 476, 586, 1052, 1975, 2272, 2344
BsuTUI	ATCGAT	1 cut(s)	117
BtgI	CCRYGG	2 cut(s)	889, 2371
BtrI	CACGTC	2 cut(s)	1289, 1860
BtsCI	GGATG	3 cut(s)	226, 782, 2106
BtsI	GCAGTG	3 cut(s)	324, 1245, 1260
BveI	ACCTGC	1 cut(s)	1048
Cac8I	GCNNGC	8 cut(s)	588, 665, 669, 844, 1197, 1542, 2063, 2419
CaiI	CAGNNNCTG	1 cut(s)	814
CfoI	GCGC	3 cut(s)	109, 129, 371
Cfr10I	RCCGGY	1 cut(s)	1697
Cfr13I	GGNCC	7 cut(s)	187, 356, 527, 584, 923, 1700, 1974
ClaI	ATCGAT	1 cut(s)	117
CseI	GACGC	1 cut(s)	60
Csp6I	GTAC	8 cut(s)	274, 744, 803, 886, 1447, 1706, 1864, 2408
CspAI	ACCGGT	1 cut(s)	1697
CspCI	CAANNNNNGTGG	2 cut(s)	1966, 2001
CviQI	GTAC	8 cut(s)	274, 744, 803, 886, 1447, 1706, 1864, 2408
EaeI	YGGCCR	3 cut(s)	182, 474, 1050
Eam1104I	CTCTTC	2 cut(s)	1521, 1753
EarI	CTCTTC	2 cut(s)	1521, 1753
Ecl136II	GAGCTC	1 cut(s)	2241
Eco130I	CCWWGG	5 cut(s)	204, 889, 1575, 2273, 2371
Eco147I	AGGCCT	1 cut(s)	2344
Eco24I	GRGCYC	5 cut(s)	336, 447, 1317, 2243, 2398
Eco31I	GGTCTC	1 cut(s)	224
Eco47I	GGWCC	4 cut(s)	187, 527, 923, 1700
Eco53kI	GAGCTC	1 cut(s)	2241
Eco57I	CTGAAG	3 cut(s)	669, 1341, 1637
Eco91I	GGTNACC	1 cut(s)	2468
EcoICRI	GAGCTC	1 cut(s)	2241
EcoO109I	RGGNCCY	2 cut(s)	527, 923
EcoO65I	GGTNACC	1 cut(s)	2468
EcoRII	CCWGG	2 cut(s)	650, 1885
EcoT14I	CCWWGG	5 cut(s)	204, 889, 1575, 2273, 2371
EcoT22I	ATGCAT	2 cut(s)	517, 1384
EcoT38I	GRGCYC	5 cut(s)	336, 447, 1317, 2243, 2398
ErhI	CCWWGG	5 cut(s)	204, 889, 1575, 2273, 2371
FalI	AAGNNNNNCTT	2 cut(s)	1473, 1505
FaqI	GGGAC	1 cut(s)	2365
FauI	CCCGC	1 cut(s)	2351
FauNDI	CATATG	1 cut(s)	580
FbaI	TGATCA	1 cut(s)	690
FblI	GTMKAC	1 cut(s)	1143
Fnu4HI	GCNGC	6 cut(s)	404, 464, 591, 615, 986, 1095
FokI	GGATG	3 cut(s)	233, 789, 2113
FriOI	GRGCYC	5 cut(s)	336, 447, 1317, 2243, 2398
Fsp4HI	GCNGC	6 cut(s)	404, 464, 591, 615, 986, 1095
FspBI	CTAG	6 cut(s)	35, 915, 1910, 2274, 2346, 2486
FspI	TGCGCA	1 cut(s)	370
GlaI	GCGC	3 cut(s)	108, 128, 370
GluI	GCNGC	6 cut(s)	404, 464, 591, 615, 986, 1095
GsaI	CCCAGC	1 cut(s)	1692
GsuI	CTGGAG	2 cut(s)	156, 1360
HaeII	RGCGCY	1 cut(s)	110
HaeIII	GGCC	9 cut(s)	184, 270, 358, 476, 586, 1052, 1975, 2272, 2344
HapII	CCGG	3 cut(s)	185, 417, 1698
HgaI	GACGC	1 cut(s)	60
HhaI	GCGC	3 cut(s)	109, 129, 371
Hin1I	GRCGYC	1 cut(s)	71
Hin6I	GCGC	3 cut(s)	107, 127, 369
HinP1I	GCGC	3 cut(s)	107, 127, 369
HincII	GTYRAC	1 cut(s)	1144
HindII	GTYRAC	1 cut(s)	1144
HindIII	AAGCTT	5 cut(s)	506, 1028, 1279, 1538, 2508
HinfI	GANTC	6 cut(s)	57, 151, 1636, 1846, 2210, 2246
HpaII	CCGG	3 cut(s)	185, 417, 1698
HphI	GGTGA	9 cut(s)	1388, 1687, 1796, 1946, 1957, 2159, 2207, 2214, 2480
Hpy166II	GTNNAC	5 cut(s)	471, 746, 1037, 1102, 1144
Hpy188III	TCNNGA	2 cut(s)	173, 229
Hpy8I	GTNNAC	5 cut(s)	471, 746, 1037, 1102, 1144
Hpy99I	CGWCG	2 cut(s)	76, 88
HpyAV	CCTTC	6 cut(s)	282, 307, 690, 1924, 2334, 2407
HpyCH4III	ACNGT	8 cut(s)	88, 386, 743, 1165, 1565, 1645, 2057, 2160
HpyCH4IV	ACGT	4 cut(s)	1288, 1859, 1966, 2085
HpySE526I	ACGT	4 cut(s)	1288, 1859, 1966, 2085
Hsp92I	GRCGYC	1 cut(s)	71
HspAI	GCGC	3 cut(s)	107, 127, 369
Ksp22I	TGATCA	1 cut(s)	690
LguI	GCTCTTC	1 cut(s)	1521
LmnI	GCTCC	9 cut(s)	682, 961, 1405, 1664, 1993, 2024, 2238, 2361, 2385
Lsp1109I	GCAGC	6 cut(s)	390, 475, 577, 626, 997, 1106
MaeI	CTAG	6 cut(s)	35, 915, 1910, 2274, 2346, 2486
MaeII	ACGT	4 cut(s)	1288, 1859, 1966, 2085
MaeIII	GTNAC	8 cut(s)	92, 1165, 1433, 1895, 1967, 2265, 2333, 2468
MfeI	CAATTG	2 cut(s)	1190, 1623
MflI	RGATCY	3 cut(s)	1590, 1795, 1821
MhlI	GDGCHC	8 cut(s)	336, 447, 651, 1317, 2198, 2243, 2294, 2398
MlsI	TGGCCA	1 cut(s)	1052
MluNI	TGGCCA	1 cut(s)	1052
MlyI	GAGTC	3 cut(s)	66, 1645, 2219
MmeI	TCCRAC	6 cut(s)	198, 283, 1085, 1325, 2177, 2404
Mox20I	TGGCCA	1 cut(s)	1052
Mph1103I	ATGCAT	2 cut(s)	517, 1384
MroXI	GAANNNNTTC	1 cut(s)	1797
MscI	TGGCCA	1 cut(s)	1052
MseI	TTAA	5 cut(s)	30, 606, 1242, 1257, 1779
MslI	CAYNNNNRTG	4 cut(s)	626, 1463, 1922, 2287
Msp20I	TGGCCA	1 cut(s)	1052
MspA1I	CMGCKG	4 cut(s)	466, 1097, 1304, 1424
MspI	CCGG	3 cut(s)	185, 417, 1698
MspR9I	CCNGG	3 cut(s)	417, 652, 1887
MunI	CAATTG	2 cut(s)	1190, 1623
MvaI	CCWGG	2 cut(s)	652, 1887
NciI	CCSGG	1 cut(s)	417
NcoI	CCATGG	2 cut(s)	889, 2371
NdeI	CATATG	1 cut(s)	580
NlaIV	GGNNCC	4 cut(s)	648, 898, 1275, 1732
NmuCI	GTSAC	4 cut(s)	1165, 1433, 2265, 2468
NsbI	TGCGCA	1 cut(s)	370
NsiI	ATGCAT	2 cut(s)	517, 1384
NspI	RCATGY	2 cut(s)	2065, 2421
NspV	TTCGAA	1 cut(s)	349
OliI	CACNNNNGTG	1 cut(s)	2287
PaeI	GCATGC	2 cut(s)	2065, 2421
PasI	CCCWGGG	1 cut(s)	651
PceI	AGGCCT	1 cut(s)	2344
PciSI	GCTCTTC	1 cut(s)	1521
PcsI	WCGNNNNNNNCGW	1 cut(s)	80
PdmI	GAANNNNTTC	1 cut(s)	1797
PfeI	GAWTC	3 cut(s)	151, 1846, 2246
PflMI	CCANNNNNTGG	1 cut(s)	756
PinAI	ACCGGT	1 cut(s)	1697
PkrI	GCNGC	6 cut(s)	405, 465, 592, 616, 987, 1096
PleI	GAGTC	3 cut(s)	65, 1644, 2218
PpsI	GAGTC	3 cut(s)	65, 1644, 2218
PpuMI	RGGWCCY	2 cut(s)	527, 923
PshBI	ATTAAT	1 cut(s)	606
Psp124BI	GAGCTC	1 cut(s)	2243
Psp5II	RGGWCCY	2 cut(s)	527, 923
Psp6I	CCWGG	2 cut(s)	650, 1885
PspEI	GGTNACC	1 cut(s)	2468
PspFI	CCCAGC	1 cut(s)	1688
PspGI	CCWGG	2 cut(s)	650, 1885
PspN4I	GGNNCC	4 cut(s)	648, 898, 1275, 1732
PspPI	GGNCC	7 cut(s)	187, 356, 527, 584, 923, 1700, 1974
PspPPI	RGGWCCY	2 cut(s)	527, 923
PstNI	CAGNNNCTG	1 cut(s)	814
PsuI	RGATCY	3 cut(s)	1590, 1795, 1821
PvuII	CAGCTG	3 cut(s)	1097, 1304, 1424
RsaI	GTAC	8 cut(s)	275, 745, 804, 887, 1448, 1707, 1865, 2409
RsaNI	GTAC	8 cut(s)	274, 744, 803, 886, 1447, 1706, 1864, 2408
RseI	CAYNNNNRTG	4 cut(s)	626, 1463, 1922, 2287
SacI	GAGCTC	1 cut(s)	2243
SalI	GTCGAC	1 cut(s)	1142
SapI	GCTCTTC	1 cut(s)	1521
SaqAI	TTAA	5 cut(s)	30, 606, 1242, 1257, 1779
SatI	GCNGC	6 cut(s)	404, 464, 591, 615, 986, 1095
Sau96I	GGNCC	7 cut(s)	187, 356, 527, 584, 923, 1700, 1974
SchI	GAGTC	3 cut(s)	66, 1645, 2219
ScrFI	CCNGG	3 cut(s)	417, 652, 1887
SduI	GDGCHC	8 cut(s)	336, 447, 651, 1317, 2198, 2243, 2294, 2398
SfcI	CTRYAG	4 cut(s)	837, 1419, 2017, 2226
SfuI	TTCGAA	1 cut(s)	349
SinI	GGWCC	4 cut(s)	187, 527, 923, 1700
SmiMI	CAYNNNNRTG	4 cut(s)	626, 1463, 1922, 2287
SmlI	CTYRAG	1 cut(s)	2285
SmoI	CTYRAG	1 cut(s)	2285
SphI	GCATGC	2 cut(s)	2065, 2421
SseBI	AGGCCT	1 cut(s)	2344
SsiI	CCGC	4 cut(s)	430, 466, 910, 2358
SspMI	CTAG	6 cut(s)	35, 915, 1910, 2274, 2346, 2486
SstI	GAGCTC	1 cut(s)	2243
StuI	AGGCCT	1 cut(s)	2344
StyD4I	CCNGG	3 cut(s)	415, 650, 1885
StyI	CCWWGG	5 cut(s)	204, 889, 1575, 2273, 2371
TaaI	ACNGT	8 cut(s)	88, 386, 743, 1165, 1565, 1645, 2057, 2160
TaiI	ACGT	4 cut(s)	1291, 1862, 1969, 2088
TaqI	TCGA	9 cut(s)	117, 149, 228, 265, 349, 549, 1143, 1599, 2445
TaqII	GACCGA	1 cut(s)	1067
TatI	WGTACW	4 cut(s)	273, 743, 1705, 2407
TfiI	GAWTC	3 cut(s)	151, 1846, 2246
Tru1I	TTAA	5 cut(s)	30, 606, 1242, 1257, 1779
Tru9I	TTAA	5 cut(s)	30, 606, 1242, 1257, 1779
TseFI	GTSAC	4 cut(s)	1165, 1433, 2265, 2468
TseI	GCWGC	6 cut(s)	403, 463, 590, 614, 985, 1094
Tsp45I	GTSAC	4 cut(s)	1165, 1433, 2265, 2468
TspDTI	ATGAA	8 cut(s)	400, 509, 1173, 1368, 1481, 1508, 2085, 2117
TspGWI	ACGGA	2 cut(s)	225, 2352
Van91I	CCANNNNNTGG	1 cut(s)	756
VpaK11BI	GGWCC	4 cut(s)	187, 527, 923, 1700
VspI	ATTAAT	1 cut(s)	606
XapI	RAATTY	2 cut(s)	753, 1601
XceI	RCATGY	2 cut(s)	2065, 2421
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1582
XmaJI	CCTAGG	1 cut(s)	2273
XmiI	GTMKAC	1 cut(s)	1143
XmnI	GAANNNNTTC	1 cut(s)	1797
XspI	CTAG	6 cut(s)	35, 915, 1910, 2274, 2346, 2486
Zsp2I	ATGCAT	2 cut(s)	517, 1384

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.