AT2G28900

Tim17/Tim22/Tim23/Pmp24 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012343
Physical Location & Seq
Reverse (-)
12414108 .. 12415661
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28900.1

Sequence Viewer

Length: 447 bp
ATGCCTTCAAGCACATTCTCCGGGACTGTTAGCACGCCGAAGCTGTCGGTGGCAGTGGACATGGGAAACCCTTTTCTCAATCTCACCGTTGATGCCTTCCTCAAGATCGGAGCTGTTGGAGTCACTAAATCTCTTGCAGAAGACACTTACAAGGCCATCGACAAAGGGAGTCTCTCCAAGAGCACTTTGGAGCATGCGCTTAAGAAGTTGTGTAAAGAAGGTGTTTACTGGGGAGCTGCTGGTGGAGTGTACATTGGAACAGAATACGGAATCGAACGTATCCGTGGCAGCAGAGATTGGAAAAACGCAATGTTAGCAGGCGCGGCGACAGGAGCAGTGCTCTCAGCGGTTGGTAAGAAAGGCAAAGACACTATTGTGATCGATGCCATTCTTGGTGGCGCGCTTGCAACCGCTTCTCAGTTCGTTAACAATCATTATTTCTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0005773 GO:0006605 GO:0006626 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006865 GO:0006886 GO:0006950 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0008509 GO:0008514 GO:0009266 GO:0009409 GO:0009507 GO:0009513 GO:0009526 GO:0009527 GO:0009536 GO:0009611 GO:0009628 GO:0009707 GO:0009719 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009753 GO:0009941 GO:0009987 GO:0010033 GO:0015031 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015833 GO:0015849 GO:0016020 GO:0016021 GO:0016043 GO:0017038 GO:0019866 GO:0019867 GO:0019904 GO:0022857 GO:0030150 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031304 GO:0031305 GO:0031966 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032592 GO:0032991 GO:0033036 GO:0033365 GO:0034220 GO:0034284 GO:0034285 GO:0034425 GO:0034426 GO:0034613 GO:0042170 GO:0042221 GO:0042802 GO:0042803 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0046942 GO:0046943 GO:0046983 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072596 GO:0072598 GO:0072655 GO:0098573 GO:0098588 GO:0098656 GO:0098796 GO:0098798 GO:0098800 GO:0098805 GO:1901700 GO:1903825 GO:1905039 GO:1990542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

148

Amino Acids

15.48

Weight (kDa)

9.16

Isoelectric Point (pI)

11.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 29 - 136 7.7e-14 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28900
fragaria_vesca FvH4_3g36080
malus_domestica MD03G1093500.v1.1 MD11G1108300.v1.1 MD11G1108500.v1.1 MD11G1108700.v1.1
prunus_persica Prupe.6G077600_v2.0.a1
pyrus_communis pycom03g07510 pycom11g08950 pycom11g08990
rosa_chinensis RchiOBHm_Chr5g0064511
rosa_laevigata RLG00000035730
rosa_multiflora Rmu_sc0003352.1_g000002
rosa_roxburghii Rroxscaffold_1G00016510
rosa_rugosa Rorug05G0365700 Rorug05G0365800
rosa_samantha Rh5AG423700 Rh5DG452400
rosa_wichuraiana Rw5G039830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 323, 401
AciI CCGC 3 cut(s) 323, 347, 411
AfaI GTAC 1 cut(s) 251
AflII CTTAAG 1 cut(s) 200
AgsI TTSAA 1 cut(s) 9
AleI CACNNNNGTG 1 cut(s) 374
AluBI AGCT 3 cut(s) 43, 113, 236
AluI AGCT 3 cut(s) 43, 113, 236
Alw21I GWGCWC 2 cut(s) 185, 342
Alw26I GTCTC 1 cut(s) 176
AoxI GGCC 1 cut(s) 153
ApeKI GCWGC 2 cut(s) 236, 288
AspLEI GCGC 4 cut(s) 199, 323, 401, 403
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 1 cut(s) 76
BbsI GAAGAC 1 cut(s) 147
Bbv12I GWGCWC 2 cut(s) 185, 342
BbvI GCAGC 2 cut(s) 223, 300
BccI CCATC 1 cut(s) 164
BciVI GTATCC 1 cut(s) 290
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 1 cut(s) 176
BfrI CTTAAG 1 cut(s) 200
BfuI GTATCC 1 cut(s) 290
BisI GCNGC 3 cut(s) 237, 289, 324
BlsI GCNGC 3 cut(s) 238, 290, 325
Bme1390I CCNGG 1 cut(s) 22
BmrFI CCNGG 1 cut(s) 22
BmrI ACTGGG 1 cut(s) 238
BmsI GCATC 2 cut(s) 82, 373
BmuI ACTGGG 1 cut(s) 238
BpiI GAAGAC 1 cut(s) 147
BplI GAGNNNNNCTC 2 cut(s) 324, 356
BpuEI CTTGAG 1 cut(s) 86
BpuMI CCSGG 1 cut(s) 22
Bsa29I ATCGAT 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 283
Bse1I ACTGG 1 cut(s) 233
Bse3DI GCAATG 1 cut(s) 315
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 283
BseMI GCAATG 1 cut(s) 315
BseMII CTCAG 2 cut(s) 357, 431
BseNI ACTGG 1 cut(s) 233
BsePI GCGCGC 1 cut(s) 399
BseXI GCAGC 2 cut(s) 223, 300
Bsh1236I CGCG 2 cut(s) 323, 401
BshFI GGCC 1 cut(s) 155
BshVI ATCGAT 1 cut(s) 381
BsiHKAI GWGCWC 2 cut(s) 185, 342
BsiSI CCGG 1 cut(s) 21
BslFI GGGAC 1 cut(s) 37
BsmAI GTCTC 1 cut(s) 176
BsmFI GGGAC 1 cut(s) 37
BsnI GGCC 1 cut(s) 155
Bsp1286I GDGCHC 2 cut(s) 185, 342
Bsp1407I TGTACA 1 cut(s) 249
Bsp143I GATC 2 cut(s) 105, 378
BspACI CCGC 3 cut(s) 323, 347, 411
BspANI GGCC 1 cut(s) 155
BspCNI CTCAG 2 cut(s) 356, 430
BspDI ATCGAT 1 cut(s) 381
BspFNI CGCG 2 cut(s) 323, 401
BspTI CTTAAG 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 315
BsrGI TGTACA 1 cut(s) 249
BsrI ACTGG 1 cut(s) 233
BssECI CCNNGG 1 cut(s) 283
BssHII GCGCGC 1 cut(s) 399
BssMI GATC 2 cut(s) 105, 378
Bst4CI ACNGT 2 cut(s) 28, 88
BstAFI CTTAAG 1 cut(s) 200
BstAUI TGTACA 1 cut(s) 249
BstC8I GCNNGC 5 cut(s) 35, 195, 319, 401, 405
BstDEI CTNAG 2 cut(s) 343, 417
BstDSI CCRYGG 1 cut(s) 283
BstFNI CGCG 2 cut(s) 323, 401
BstHHI GCGC 4 cut(s) 199, 323, 401, 403
BstKTI GATC 2 cut(s) 108, 381
BstMAI GTCTC 1 cut(s) 176
BstMBI GATC 2 cut(s) 105, 378
BstMWI GCNNNNNNNGC 3 cut(s) 314, 323, 332
BstNSI RCATGY 1 cut(s) 197
BstSCI CCNGG 1 cut(s) 20
BstUI CGCG 2 cut(s) 323, 401
BstV1I GCAGC 2 cut(s) 223, 300
BstV2I GAAGAC 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 381
BsuI GTATCC 1 cut(s) 290
BsuRI GGCC 1 cut(s) 155
BsuTUI ATCGAT 1 cut(s) 381
BtgI CCRYGG 1 cut(s) 283
BtsI GCAGTG 2 cut(s) 60, 342
BtsIMutI CAGTG 2 cut(s) 60, 342
Cac8I GCNNGC 5 cut(s) 35, 195, 319, 401, 405
CfoI GCGC 4 cut(s) 199, 323, 401, 403
ClaI ATCGAT 1 cut(s) 381
Csp6I GTAC 1 cut(s) 250
CviAII CATG 2 cut(s) 61, 194
CviJI RGCY 4 cut(s) 43, 113, 155, 236
CviKI_1 RGCY 4 cut(s) 43, 113, 155, 236
CviQI GTAC 1 cut(s) 250
DdeI CTNAG 2 cut(s) 343, 417
DpnI GATC 2 cut(s) 107, 380
DpnII GATC 2 cut(s) 105, 378
FaeI CATG 2 cut(s) 64, 197
FaiI YATR 2 cut(s) 62, 195
FaqI GGGAC 1 cut(s) 37
FatI CATG 2 cut(s) 60, 193
Fnu4HI GCNGC 3 cut(s) 237, 289, 324
Fsp4HI GCNGC 3 cut(s) 237, 289, 324
GlaI GCGC 4 cut(s) 198, 322, 400, 402
GluI GCNGC 3 cut(s) 237, 289, 324
HaeIII GGCC 1 cut(s) 155
HapII CCGG 1 cut(s) 21
HhaI GCGC 4 cut(s) 199, 323, 401, 403
Hin1II CATG 2 cut(s) 64, 197
Hin6I GCGC 4 cut(s) 197, 321, 399, 401
HinP1I GCGC 4 cut(s) 197, 321, 399, 401
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HinfI GANTC 3 cut(s) 120, 169, 270
HpaI GTTAAC 1 cut(s) 427
HpaII CCGG 1 cut(s) 21
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 4 cut(s) 58, 226, 250, 427
Hpy188I TCNGA 1 cut(s) 110
Hpy188III TCNNGA 1 cut(s) 103
Hpy8I GTNNAC 4 cut(s) 58, 226, 250, 427
HpyAV CCTTC 3 cut(s) 15, 106, 212
HpyCH4III ACNGT 2 cut(s) 28, 88
HpyCH4IV ACGT 1 cut(s) 277
HpyCH4V TGCA 2 cut(s) 137, 407
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 323, 332
HpyF3I CTNAG 2 cut(s) 343, 417
HpySE526I ACGT 1 cut(s) 277
Hsp92II CATG 2 cut(s) 64, 197
HspAI GCGC 4 cut(s) 197, 321, 399, 401
KspAI GTTAAC 1 cut(s) 427
Kzo9I GATC 2 cut(s) 105, 378
LmnI GCTCC 4 cut(s) 110, 190, 233, 332
LpnPI CCDG 5 cut(s) 34, 214, 225, 303, 315
Lsp1109I GCAGC 2 cut(s) 223, 300
LweI GCATC 2 cut(s) 82, 373
MaeII ACGT 1 cut(s) 277
MaeIII GTNAC 1 cut(s) 121
MalI GATC 2 cut(s) 107, 380
MboI GATC 2 cut(s) 105, 378
MboII GAAGA 1 cut(s) 152
MhlI GDGCHC 2 cut(s) 185, 342
MlyI GAGTC 2 cut(s) 129, 178
MmeI TCCRAC 1 cut(s) 97
MnlI CCTC 1 cut(s) 110
MseI TTAA 2 cut(s) 201, 426
MslI CAYNNNNRTG 1 cut(s) 374
MspA1I CMGCKG 1 cut(s) 347
MspCI CTTAAG 1 cut(s) 200
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 1 cut(s) 22
MvnI CGCG 2 cut(s) 323, 401
MwoI GCNNNNNNNGC 3 cut(s) 314, 323, 332
NciI CCSGG 1 cut(s) 22
NdeII GATC 2 cut(s) 105, 378
NlaIII CATG 2 cut(s) 64, 197
NmuCI GTSAC 1 cut(s) 121
NspI RCATGY 1 cut(s) 197
OliI CACNNNNGTG 1 cut(s) 374
PaeI GCATGC 1 cut(s) 197
PauI GCGCGC 1 cut(s) 399
PfeI GAWTC 1 cut(s) 270
PfoI TCCNGGA 1 cut(s) 20
PkrI GCNGC 3 cut(s) 238, 290, 325
PleI GAGTC 2 cut(s) 128, 177
PpsI GAGTC 2 cut(s) 128, 177
PteI GCGCGC 1 cut(s) 399
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
RseI CAYNNNNRTG 1 cut(s) 374
SaqAI TTAA 2 cut(s) 201, 426
SatI GCNGC 3 cut(s) 237, 289, 324
Sau3AI GATC 2 cut(s) 105, 378
SchI GAGTC 2 cut(s) 129, 178
ScrFI CCNGG 1 cut(s) 22
SduI GDGCHC 2 cut(s) 185, 342
SetI ASST 5 cut(s) 45, 115, 223, 238, 280
SfaNI GCATC 2 cut(s) 82, 373
SmiMI CAYNNNNRTG 1 cut(s) 374
SmlI CTYRAG 2 cut(s) 101, 200
SmoI CTYRAG 2 cut(s) 101, 200
SphI GCATGC 1 cut(s) 197
SsiI CCGC 3 cut(s) 323, 347, 411
StyD4I CCNGG 1 cut(s) 20
TaaI ACNGT 2 cut(s) 28, 88
TaiI ACGT 1 cut(s) 280
TaqI TCGA 3 cut(s) 159, 273, 381
TatI WGTACW 1 cut(s) 249
TauI GCSGC 1 cut(s) 326
TfiI GAWTC 1 cut(s) 270
Tru1I TTAA 2 cut(s) 201, 426
Tru9I TTAA 2 cut(s) 201, 426
TscAI CASTG 2 cut(s) 60, 342
TseFI GTSAC 1 cut(s) 121
TseI GCWGC 2 cut(s) 236, 288
Tsp45I GTSAC 1 cut(s) 121
TspGWI ACGGA 2 cut(s) 272, 282
TspRI CASTG 2 cut(s) 60, 342
Vha464I CTTAAG 1 cut(s) 200
XceI RCATGY 1 cut(s) 197
XcmI CCANNNNNNNNNTGG 1 cut(s) 184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.