MD03G1093500.v1.1

Outer envelope pore protein 16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012343
Physical Location & Seq
Forward (+)
7802198 .. 7804690
2493 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1093500.v1.1.491

Sequence Viewer

Length: 441 bp
ATGCCGAGAACCAGTTTCGCAGGGTCGCTAACAGGGCCGAAGGTCGACGTCATGATCGACTTGGGCAACCCCTTCTTCAACCACACTGTCGATGGCTTCTTGAAGATCGGAACCGTCGCTGCTACCAGAGTTGTTGCAGAGGATGCTTTCTCGGCGGCCAAGAAAGGGAGAATTTCAAGTCACGATTTTGAGCACACTTTGAAGAAGATGTGTAAAGAAGGTGCATATTGGGGAACCATAGCTGGTGTGTACGTGGGAGCAGAGTATGGTATTGAGAGGGTCCGTGGACATAGAGACTGGAAGAATGCCATGCTCGGGGGTGCATTGACAGGGGCTCTAGTATCTGCAGCCAGCCACAAGAACACGGATAAAGTTCTGGTGGATGCAATTACAGGAGGTGCCATTGCAACTGCTGCAGAATTCATTAACTATCTCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0005773 GO:0006605 GO:0006626 GO:0006810 GO:0006811 GO:0006820 GO:0006839 GO:0006865 GO:0006886 GO:0006950 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0008509 GO:0008514 GO:0009266 GO:0009409 GO:0009507 GO:0009513 GO:0009526 GO:0009527 GO:0009536 GO:0009611 GO:0009628 GO:0009707 GO:0009719 GO:0009725 GO:0009743 GO:0009744 GO:0009746 GO:0009749 GO:0009753 GO:0009941 GO:0009987 GO:0010033 GO:0015031 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015833 GO:0015849 GO:0016020 GO:0016021 GO:0016043 GO:0017038 GO:0019866 GO:0019867 GO:0019904 GO:0022857 GO:0030150 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031304 GO:0031305 GO:0031966 GO:0031967 GO:0031968 GO:0031969 GO:0031975 GO:0032592 GO:0032991 GO:0033036 GO:0033365 GO:0034220 GO:0034284 GO:0034285 GO:0034425 GO:0034426 GO:0034613 GO:0042170 GO:0042221 GO:0042802 GO:0042803 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045036 GO:0045037 GO:0045184 GO:0046907 GO:0046942 GO:0046943 GO:0046983 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070585 GO:0070727 GO:0071702 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072596 GO:0072598 GO:0072655 GO:0098573 GO:0098588 GO:0098656 GO:0098796 GO:0098798 GO:0098800 GO:0098805 GO:1901700 GO:1903825 GO:1905039 GO:1990542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tim17

Protein Analysis

147

Amino Acids

15.49

Weight (kDa)

8.71

Isoelectric Point (pI)

4.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 37 - 136 4.4e-13 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012343)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28900
fragaria_vesca FvH4_3g36080
malus_domestica MD03G1093500.v1.1 MD11G1108300.v1.1 MD11G1108500.v1.1 MD11G1108700.v1.1
prunus_persica Prupe.6G077600_v2.0.a1
pyrus_communis pycom03g07510 pycom11g08950 pycom11g08990
rosa_chinensis RchiOBHm_Chr5g0064511
rosa_laevigata RLG00000035730
rosa_multiflora Rmu_sc0003352.1_g000002
rosa_roxburghii Rroxscaffold_1G00016510
rosa_rugosa Rorug05G0365700 Rorug05G0365800
rosa_samantha Rh5AG423700 Rh5DG452400
rosa_wichuraiana Rw5G039830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 51
AccB1I GGYRCC 1 cut(s) 398
AccI GTMKAC 1 cut(s) 45
AciI CCGC 1 cut(s) 155
AcoI YGGCCR 1 cut(s) 156
AcsI RAATTY 2 cut(s) 171, 419
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 1 cut(s) 251
AfiI CCNNNNNNNGG 2 cut(s) 165, 315
AgsI TTSAA 4 cut(s) 79, 103, 177, 202
AluBI AGCT 1 cut(s) 242
AluI AGCT 1 cut(s) 242
Alw21I GWGCWC 1 cut(s) 195
Alw26I GTCTC 1 cut(s) 288
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 2 cut(s) 35, 156
ApeKI GCWGC 3 cut(s) 119, 347, 413
ApoI RAATTY 2 cut(s) 171, 419
AspS9I GGNCC 2 cut(s) 35, 280
AsuHPI GGTGA 1 cut(s) 427
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 1 cut(s) 280
BanI GGYRCC 1 cut(s) 398
BanII GRGCYC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 195
BbvI GCAGC 3 cut(s) 106, 359, 400
BccI CCATC 1 cut(s) 86
BcoDI GTCTC 1 cut(s) 288
BfaI CTAG 1 cut(s) 338
BfmI CTRYAG 2 cut(s) 345, 414
BisI GCNGC 4 cut(s) 120, 156, 348, 414
BlsI GCNGC 4 cut(s) 121, 157, 349, 415
Bme18I GGWCC 1 cut(s) 280
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 2 cut(s) 35, 280
BmiI GGNNCC 4 cut(s) 112, 235, 281, 400
BmsI GCATC 2 cut(s) 133, 373
BsaAI YACGTR 1 cut(s) 253
BsaHI GRCGYC 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 283
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 315
Bse1I ACTGG 2 cut(s) 12, 302
Bse3DI GCAATG 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 2 cut(s) 148, 388
BseLI CCNNNNNNNGG 2 cut(s) 165, 315
BseMI GCAATG 1 cut(s) 402
BseNI ACTGG 2 cut(s) 12, 302
BseXI GCAGC 3 cut(s) 106, 359, 400
BshFI GGCC 2 cut(s) 37, 158
BshNI GGYRCC 1 cut(s) 398
BsiHKAI GWGCWC 1 cut(s) 195
BsiHKCI CYCGRG 1 cut(s) 314
BslI CCNNNNNNNGG 2 cut(s) 165, 315
BsmAI GTCTC 1 cut(s) 288
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 2 cut(s) 37, 158
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 2 cut(s) 195, 337
Bsp143I GATC 2 cut(s) 54, 105
BspACI CCGC 1 cut(s) 155
BspANI GGCC 2 cut(s) 37, 158
BspHI TCATGA 1 cut(s) 51
BspLI GGNNCC 4 cut(s) 112, 235, 281, 400
BspMAI CTGCAG 2 cut(s) 349, 418
BspT107I GGYRCC 1 cut(s) 398
BsrDI GCAATG 1 cut(s) 402
BsrI ACTGG 2 cut(s) 12, 302
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 2 cut(s) 54, 105
BssNI GRCGYC 1 cut(s) 48
Bst4CI ACNGT 2 cut(s) 88, 115
BstACI GRCGYC 1 cut(s) 48
BstAPI GCANNNNNTGC 2 cut(s) 143, 413
BstBAI YACGTR 1 cut(s) 253
BstC8I GCNNGC 1 cut(s) 352
BstDSI CCRYGG 1 cut(s) 283
BstF5I GGATG 2 cut(s) 148, 388
BstKTI GATC 2 cut(s) 57, 108
BstMAI GTCTC 1 cut(s) 288
BstMBI GATC 2 cut(s) 54, 105
BstMWI GCNNNNNNNGC 4 cut(s) 34, 143, 152, 413
BstSFI CTRYAG 2 cut(s) 345, 414
BstV1I GCAGC 3 cut(s) 106, 359, 400
BsuRI GGCC 2 cut(s) 37, 158
BtgI CCRYGG 1 cut(s) 283
BtsCI GGATG 2 cut(s) 148, 388
BtsIMutI CAGTG 1 cut(s) 84
Cac8I GCNNGC 1 cut(s) 352
CciI TCATGA 1 cut(s) 51
Cfr13I GGNCC 2 cut(s) 35, 280
Csp6I GTAC 1 cut(s) 250
CviAII CATG 2 cut(s) 52, 310
CviJI RGCY 7 cut(s) 37, 96, 158, 242, 335, 350, 354
CviKI_1 RGCY 7 cut(s) 37, 96, 158, 242, 335, 350, 354
CviQI GTAC 1 cut(s) 250
DpnI GATC 2 cut(s) 56, 107
DpnII GATC 2 cut(s) 54, 105
EaeI YGGCCR 1 cut(s) 156
Eco24I GRGCYC 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 280
Eco88I CYCGRG 1 cut(s) 314
EcoRI GAATTC 1 cut(s) 419
EcoT38I GRGCYC 1 cut(s) 337
FaeI CATG 2 cut(s) 55, 313
FaiI YATR 6 cut(s) 53, 226, 239, 267, 291, 311
FatI CATG 2 cut(s) 51, 309
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 4 cut(s) 120, 156, 348, 414
FokI GGATG 2 cut(s) 155, 395
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 4 cut(s) 120, 156, 348, 414
FspBI CTAG 1 cut(s) 338
GluI GCNGC 4 cut(s) 120, 156, 348, 414
HaeIII GGCC 2 cut(s) 37, 158
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 2 cut(s) 55, 313
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HphI GGTGA 1 cut(s) 427
Hpy166II GTNNAC 3 cut(s) 46, 250, 287
Hpy188I TCNGA 1 cut(s) 110
Hpy188III TCNNGA 3 cut(s) 52, 100, 182
Hpy8I GTNNAC 3 cut(s) 46, 250, 287
Hpy99I CGWCG 2 cut(s) 50, 119
HpyAV CCTTC 3 cut(s) 34, 82, 212
HpyCH4III ACNGT 2 cut(s) 88, 115
HpyCH4IV ACGT 2 cut(s) 48, 252
HpyCH4V TGCA 7 cut(s) 137, 224, 323, 347, 386, 407, 416
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 143, 152, 413
HpySE526I ACGT 2 cut(s) 48, 252
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 2 cut(s) 55, 313
Kzo9I GATC 2 cut(s) 54, 105
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 3 cut(s) 106, 359, 400
LweI GCATC 2 cut(s) 133, 373
MaeI CTAG 1 cut(s) 338
MaeII ACGT 2 cut(s) 48, 252
MaeIII GTNAC 1 cut(s) 179
MalI GATC 2 cut(s) 56, 107
MboI GATC 2 cut(s) 54, 105
MboII GAAGA 5 cut(s) 67, 115, 214, 217, 313
MhlI GDGCHC 2 cut(s) 195, 337
MluCI AATT 3 cut(s) 171, 387, 419
MnlI CCTC 3 cut(s) 133, 270, 389
MseI TTAA 1 cut(s) 426
Mva1269I GAATGC 1 cut(s) 310
MwoI GCNNNNNNNGC 4 cut(s) 34, 143, 152, 413
NdeII GATC 2 cut(s) 54, 105
NlaIII CATG 2 cut(s) 55, 313
NlaIV GGNNCC 4 cut(s) 112, 235, 281, 400
NmeAIII GCCGAG 2 cut(s) 30, 131
NmuCI GTSAC 1 cut(s) 179
PagI TCATGA 1 cut(s) 51
PcsI WCGNNNNNNNCGW 1 cut(s) 54
PctI GAATGC 1 cut(s) 310
PkrI GCNGC 4 cut(s) 121, 157, 349, 415
Ppu21I YACGTR 1 cut(s) 253
PspN4I GGNNCC 4 cut(s) 112, 235, 281, 400
PspPI GGNCC 2 cut(s) 35, 280
PstI CTGCAG 2 cut(s) 349, 418
RsaI GTAC 1 cut(s) 251
RsaNI GTAC 1 cut(s) 250
SalI GTCGAC 1 cut(s) 44
SaqAI TTAA 1 cut(s) 426
SatI GCNGC 4 cut(s) 120, 156, 348, 414
Sau3AI GATC 2 cut(s) 54, 105
Sau96I GGNCC 2 cut(s) 35, 280
SduI GDGCHC 2 cut(s) 195, 337
SetI ASST 7 cut(s) 45, 51, 223, 244, 255, 400, 440
SfaNI GCATC 2 cut(s) 133, 373
SfcI CTRYAG 2 cut(s) 345, 414
SinI GGWCC 1 cut(s) 280
Sse9I AATT 3 cut(s) 171, 387, 419
SsiI CCGC 1 cut(s) 155
SspMI CTAG 1 cut(s) 338
TaaI ACNGT 2 cut(s) 88, 115
TaiI ACGT 2 cut(s) 51, 255
TaqI TCGA 3 cut(s) 45, 57, 90
TasI AATT 3 cut(s) 171, 387, 419
TauI GCSGC 1 cut(s) 158
Tru1I TTAA 1 cut(s) 426
Tru9I TTAA 1 cut(s) 426
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 1 cut(s) 179
TseI GCWGC 3 cut(s) 119, 347, 413
Tsp45I GTSAC 1 cut(s) 179
TspDTI ATGAA 1 cut(s) 412
TspGWI ACGGA 2 cut(s) 272, 380
TspRI CASTG 1 cut(s) 91
VpaK11BI GGWCC 1 cut(s) 280
XapI RAATTY 2 cut(s) 171, 419
XcmI CCANNNNNNNNNTGG 1 cut(s) 89
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 1 cut(s) 338
ZraI GACGTC 1 cut(s) 49
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.