AT2G31500

ATP binding calcium ion binding calmodulin-dependent protein kinase kinase protein kinase protein serine threonine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0017037
Physical Location & Seq
Forward (+)
13413738 .. 13416602
2865 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G31500.1

Sequence Viewer

Length: 1749 bp
ATGGGAAGTTGTGTTTCGTCGCCATTGAAAGGCTCTCCTTTTGGAAAGAGACCGGTAAGAAGGCGACACAGTAGTAATAGCAGAACATCCTCTGTTCCTCGTTTCGATTCTTCAACTAATCTTTCCCGGAGATTGATTTTCCAGCCACCAAGCCGCGTTCTCCCCGAGCCTATCGGTGATGGAATTCACCTCAAGTATGATTTGGGAAAAGAACTAGGCCGTGGTGAATTTGGTGTCACTCATGAATGTATCGAGATCAGTACCCGAGAAAGATTTGCGTGTAAGAGGATATCGAAGGAGAAGCTAAGGACGGAGATAGATGTGGAGGATGTGAGAAGAGAGGTTGAGATCATGAGATGTTTACCAAAACATCCTAATATTGTTAGCTTTAAGGAAGCTTTTGAGGACAAAGACGCTGTCTATCTTGTCATGGAGATTTGTGAAGGAGGTGAACTTTTTGACCGCATTGTCTCTAGAGGGCATTACACTGAACGCGCCGCTGCATCTGTTGCTAAAACCATTCTTGAAGTTGTCAAGGTGTGTCATGAACATGGAGTGATTCATCGTGATCTTAAACCAGAGAACTTCCTCTTTTCGAATGGAACTGAGACTGCACAACTCAAAGCCATTGATTTTGGTCTCTCCATTTTCTTCAAACCGGCCCAGCGGTTCAATGAGATTGTGGGAAGTCCTTACTACATGGCTCCAGAGGTTTTGAGACGAAATTACGGTCCTGAGATCGATGTTTGGAGTGCTGGTGTTATACTCTATATCTTGCTTTGTGGTGTTCCTCCCTTTTGGGCAGAAACCGAGGAAGGAATCGCGCACGCTATTGTGAGAGGGAACATCGATTTCGAGAGAGATCCATGGCCAAAAGTCTCACATGAAGCCAAGGAACTCGTTAAGAACATGCTTGATGCAAATCCTTATAGCAGACTCACAGTCCAAGAAGTGCTTGAACATCCATGGATCCGGAACGCAGAGAGAGCACCAAATGTGAATCTTGGAGATAACGTCAGGACCAAGATTCAACAATTCTTGTTGATGAACAGGTTCAAGAAGAAAGTCCTCAGGATTGTAGCGGACAATCTACCAAACGAGGAGATAGCAGCGATAGTACAGATGTTTCAAACAATGGACACTGATAAGAATGGTCACTTGACTTTTGAGGAGCTAAGAGATGGACTGAAGAAGATTGGACAAGTTGTTCCCGATGGCGATGTGAAGATGCTAATGGATGCAGCGGATACAGATGGAAATGGGATGTTGAGCTGTGATGAGTTTGTGACACTCTCAATACATTTGAAGAGAATGGGATGTGATGAGCATTTGCAGGAAGCATTCAAGTATTTTGACAAGAACGGGAACGGATTTATCGAGCTAGATGAGCTCAAGGTAGCTCTTTGCGATGATAAGCTAGGCCACGCTAACGGTAACGACCAGTGGATCAAAGATATCTTCTTTGACGTCGACCTCAACAAGGATGGACGGATAAGCTTTGATGAGTTTAAGGCAATGATGAAATCAGGGACGGATTGGAAAATGGCGTCGAGGCAGTACTCGAGGGCGTTGTTGAATGCTCTAAGCATCAAGATGTTCAAAGAAGATTTTGGGGACAATGGTCCTAAGTCACATTCCATGGAGTTCCCTATAGCAAGGAAGAGGGCTAAGCTGCTTGATGCTCCCAAGAACAAATCAATGGAGCTTCAGATCTCCAAAACCTATAAACCCTCAGGTCTAAGAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010769 GO:0010857 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043269 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0046777 GO:0047484 GO:0048519 GO:0048583 GO:0048585 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080092 GO:0080134 GO:0097305 GO:0097306 GO:0140096 GO:1901000 GO:1901001 GO:1901016 GO:1901379 GO:1901564 GO:1901700 GO:1901701 GO:1901979 GO:1904062 GO:2000241 GO:2001257
KEGG Pathways
Metabolic & Signaling
ko04626 ko05145 map04626 map05145
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

582

Amino Acids

66.24

Weight (kDa)

6.98

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 66 - 324 2.6e-79 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 68 - 320 1.8e-27 Protein tyrosine and serine/threonine kinase
EF-hand_7 PF13499 371 - 431 3.9e-11 EF-hand domain pair
EF-hand_1 PF00036 373 - 398 7.9e-07 EF hand domain
EF-hand_8 PF13833 385 - 431 2.7e-08 EF-hand domain pair
EF-hand_7 PF13499 442 - 508 4.7e-13 EF-hand domain pair
EF-hand_1 PF00036 444 - 468 1.1e-06 EF hand domain
EF-hand_6 PF13405 444 - 469 1.4e-06 EF-hand domain
EF-hand_1 PF00036 483 - 509 2.7e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31500
fragaria_vesca FvH4_4g11140
malus_domestica MD09G1092200.v1.1 MD17G1082100.v1.1
prunus_persica Prupe.1G190700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0407071
rosa_laevigata RLG00000008739
rosa_multiflora Rmu_sc0000600.1_g000002
rosa_rugosa Rorug04G0077800
rosa_samantha Rh4AG142500 Rh4CG149000 Rh4DG136600
rosa_wichuraiana Rw4G011610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 467
AatII GACGTC 1 cut(s) 1470
AccI GTMKAC 1 cut(s) 1470
AccII CGCG 3 cut(s) 156, 495, 824
AccIII TCCGGA 1 cut(s) 972
AciI CCGC 6 cut(s) 154, 463, 498, 667, 1082, 1244
AclWI GGATC 4 cut(s) 857, 964, 977, 1454
AcoI YGGCCR 1 cut(s) 869
AcsI RAATTY 2 cut(s) 183, 227
AcuI CTGAAG 2 cut(s) 1208, 1691
AcyI GRCGYC 2 cut(s) 1467, 1547
AfaI GTAC 3 cut(s) 262, 1119, 1559
AfiI CCNNNNNNNGG 2 cut(s) 29, 1480
AgeI ACCGGT 1 cut(s) 52
AhdI GACNNNNNGTC 1 cut(s) 941
AloI GAACNNNNNNTCC 2 cut(s) 1191, 1223
Alw21I GWGCWC 2 cut(s) 991, 1392
Alw26I GTCTC 6 cut(s) 43, 475, 602, 644, 712, 883
AlwI GGATC 4 cut(s) 857, 964, 977, 1454
Ama87I CYCGRG 3 cut(s) 164, 264, 1561
Aor13HI TCCGGA 1 cut(s) 972
AoxI GGCC 4 cut(s) 217, 660, 869, 1420
ApeKI GCWGC 4 cut(s) 500, 1109, 1241, 1672
ApoI RAATTY 2 cut(s) 183, 227
ArsI GACNNNNNNTTYG 4 cut(s) 402, 434, 715, 747
AsiGI ACCGGT 1 cut(s) 52
Asp700I GAANNNNTTC 1 cut(s) 1052
AspLEI GCGC 2 cut(s) 497, 826
AspS9I GGNCC 4 cut(s) 661, 731, 1020, 1622
AsuC2I CCSGG 1 cut(s) 127
AsuHPI GGTGA 4 cut(s) 179, 188, 236, 461
AsuII TTCGAA 1 cut(s) 596
AvaI CYCGRG 3 cut(s) 164, 264, 1561
AvaII GGWCC 3 cut(s) 731, 1020, 1622
AxyI CCTNAGG 2 cut(s) 1070, 1732
BalI TGGCCA 1 cut(s) 871
BamHI GGATCC 1 cut(s) 969
BanII GRGCYC 1 cut(s) 1392
Bbv12I GWGCWC 2 cut(s) 991, 1392
BbvI GCAGC 4 cut(s) 487, 1121, 1253, 1659
BccI CCATC 5 cut(s) 173, 1175, 1208, 1247, 1478
BceAI ACGGC 1 cut(s) 204
BciVI GTATCC 1 cut(s) 1240
BcnI CCSGG 1 cut(s) 127
BcoDI GTCTC 6 cut(s) 43, 475, 602, 644, 712, 883
BfaI CTAG 4 cut(s) 215, 474, 1382, 1418
BfmI CTRYAG 1 cut(s) 1650
BfuI GTATCC 1 cut(s) 1240
BglII AGATCT 1 cut(s) 1710
BisI GCNGC 6 cut(s) 154, 498, 501, 1110, 1242, 1673
BlpI GCTNAGC 1 cut(s) 1668
BlsI GCNGC 6 cut(s) 155, 499, 502, 1111, 1243, 1674
BmcAI AGTACT 1 cut(s) 1559
Bme1390I CCNGG 1 cut(s) 127
Bme18I GGWCC 3 cut(s) 731, 1020, 1622
BmeRI GACNNNNNGTC 1 cut(s) 941
BmeT110I CYCGRG 3 cut(s) 164, 264, 1561
BmgT120I GGNCC 4 cut(s) 661, 731, 1020, 1622
BmiI GGNNCC 2 cut(s) 705, 971
BmrFI CCNGG 1 cut(s) 127
BmsI GCATC 6 cut(s) 512, 907, 1218, 1228, 1596, 1669
BoxI GACNNNNGTC 1 cut(s) 1620
BpmI CTGGAG 1 cut(s) 690
Bpu10I CCTNAGC 1 cut(s) 305
Bpu1102I GCTNAGC 1 cut(s) 1668
Bpu14I TTCGAA 1 cut(s) 596
BpuEI CTTGAG 2 cut(s) 176, 1376
BpuMI CCSGG 1 cut(s) 127
Bsa29I ATCGAT 2 cut(s) 741, 849
BsaBI GATNNNNATC 1 cut(s) 921
BsaHI GRCGYC 2 cut(s) 1467, 1547
BsaI GGTCTC 2 cut(s) 43, 644
BsaJI CCNNGG 6 cut(s) 220, 810, 866, 891, 965, 1638
BsaWI WCCGGW 2 cut(s) 52, 972
BsaXI ACNNNNNCTCC 4 cut(s) 742, 772, 999, 1029
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 1480
Bse118I RCCGGY 2 cut(s) 52, 658
Bse1I ACTGG 1 cut(s) 1441
Bse21I CCTNAGG 2 cut(s) 1070, 1732
Bse3DI GCAATG 1 cut(s) 1521
Bse8I GATNNNNATC 1 cut(s) 921
BseAI TCCGGA 1 cut(s) 972
BseCI ATCGAT 2 cut(s) 741, 849
BseDI CCNNGG 6 cut(s) 220, 810, 866, 891, 965, 1638
BseGI GGATG 8 cut(s) 86, 334, 370, 961, 1243, 1269, 1322, 1489
BseJI GATNNNNATC 1 cut(s) 921
BseLI CCNNNNNNNGG 2 cut(s) 29, 1480
BseMI GCAATG 1 cut(s) 1521
BseMII CTCAG 4 cut(s) 597, 726, 1084, 1746
BseNI ACTGG 1 cut(s) 1441
BseRI GAGGAG 2 cut(s) 1115, 1184
BseXI GCAGC 4 cut(s) 487, 1121, 1253, 1659
BseYI CCCAGC 1 cut(s) 663
BsgI GTGCAG 1 cut(s) 597
Bsh1236I CGCG 3 cut(s) 156, 495, 824
BshFI GGCC 4 cut(s) 219, 662, 871, 1422
BshTI ACCGGT 1 cut(s) 52
BshVI ATCGAT 2 cut(s) 741, 849
BsiHKAI GWGCWC 2 cut(s) 991, 1392
BsiHKCI CYCGRG 3 cut(s) 164, 264, 1561
BsiSI CCGG 4 cut(s) 53, 127, 659, 973
BslFI GGGAC 2 cut(s) 1543, 1628
BslI CCNNNNNNNGG 2 cut(s) 29, 1480
BsmAI GTCTC 6 cut(s) 43, 475, 602, 644, 712, 883
BsmBI CGTCTC 1 cut(s) 712
BsmFI GGGAC 2 cut(s) 1543, 1628
BsmI GAATGC 2 cut(s) 1340, 1582
BsnI GGCC 4 cut(s) 219, 662, 871, 1422
Bso31I GGTCTC 2 cut(s) 43, 644
BsoBI CYCGRG 3 cut(s) 164, 264, 1561
Bsp119I TTCGAA 1 cut(s) 596
Bsp1286I GDGCHC 2 cut(s) 991, 1392
Bsp13I TCCGGA 1 cut(s) 972
Bsp143I GATC 8 cut(s) 255, 348, 568, 738, 862, 969, 1446, 1710
Bsp1720I GCTNAGC 1 cut(s) 1668
Bsp19I CCATGG 3 cut(s) 866, 965, 1638
BspACI CCGC 6 cut(s) 154, 463, 498, 667, 1082, 1244
BspANI GGCC 4 cut(s) 219, 662, 871, 1422
BspCNI CTCAG 4 cut(s) 598, 727, 1083, 1745
BspDI ATCGAT 2 cut(s) 741, 849
BspEI TCCGGA 1 cut(s) 972
BspFNI CGCG 3 cut(s) 156, 495, 824
BspHI TCATGA 3 cut(s) 241, 351, 544
BspLI GGNNCC 2 cut(s) 705, 971
BspPI GGATC 4 cut(s) 857, 964, 977, 1454
BspT104I TTCGAA 1 cut(s) 596
BspTNI GGTCTC 2 cut(s) 43, 644
BsrDI GCAATG 1 cut(s) 1521
BsrFI RCCGGY 2 cut(s) 52, 658
BsrI ACTGG 1 cut(s) 1441
BssAI RCCGGY 2 cut(s) 52, 658
BssECI CCNNGG 6 cut(s) 220, 810, 866, 891, 965, 1638
BssMI GATC 8 cut(s) 255, 348, 568, 738, 862, 969, 1446, 1710
BssNI GRCGYC 2 cut(s) 1467, 1547
BssT1I CCWWGG 4 cut(s) 866, 891, 965, 1638
Bst4CI ACNGT 4 cut(s) 71, 731, 943, 1433
Bst6I CTCTTC 3 cut(s) 331, 1301, 1655
BstACI GRCGYC 2 cut(s) 1467, 1547
BstAPI GCANNNNNTGC 1 cut(s) 509
BstBI TTCGAA 1 cut(s) 596
BstC8I GCNNGC 1 cut(s) 828
BstDSI CCRYGG 4 cut(s) 220, 866, 965, 1638
BstENI CCTNNNNNAGG 1 cut(s) 1478
BstF5I GGATG 8 cut(s) 86, 334, 370, 961, 1243, 1269, 1322, 1489
BstFNI CGCG 3 cut(s) 156, 495, 824
BstHHI GCGC 2 cut(s) 497, 826
BstKTI GATC 8 cut(s) 258, 351, 571, 741, 865, 972, 1449, 1713
BstMAI GTCTC 6 cut(s) 43, 475, 602, 644, 712, 883
BstMBI GATC 8 cut(s) 255, 348, 568, 738, 862, 969, 1446, 1710
BstMWI GCNNNNNNNGC 3 cut(s) 509, 986, 1387
BstNSI RCATGY 1 cut(s) 913
BstPAI GACNNNNGTC 1 cut(s) 1620
BstSCI CCNGG 1 cut(s) 125
BstSFI CTRYAG 1 cut(s) 1650
BstUI CGCG 3 cut(s) 156, 495, 824
BstV1I GCAGC 4 cut(s) 487, 1121, 1253, 1659
BstX2I RGATCY 3 cut(s) 862, 969, 1710
BstYI RGATCY 3 cut(s) 862, 969, 1710
Bsu15I ATCGAT 2 cut(s) 741, 849
Bsu36I CCTNAGG 2 cut(s) 1070, 1732
BsuI GTATCC 1 cut(s) 1240
BsuRI GGCC 4 cut(s) 219, 662, 871, 1422
BsuTUI ATCGAT 2 cut(s) 741, 849
BtgI CCRYGG 4 cut(s) 220, 866, 965, 1638
BtgZI GCGATG 2 cut(s) 1233, 1422
BtsCI GGATG 8 cut(s) 86, 334, 370, 961, 1243, 1269, 1322, 1489
BtsIMutI CAGTG 3 cut(s) 486, 1140, 1448
Cac8I GCNNGC 1 cut(s) 828
CciI TCATGA 3 cut(s) 241, 351, 544
CfoI GCGC 2 cut(s) 497, 826
Cfr10I RCCGGY 2 cut(s) 52, 658
Cfr13I GGNCC 4 cut(s) 661, 731, 1020, 1622
ClaI ATCGAT 2 cut(s) 741, 849
CseI GACGC 2 cut(s) 422, 1536
Csp6I GTAC 3 cut(s) 261, 1118, 1558
CspAI ACCGGT 1 cut(s) 52
CviQI GTAC 3 cut(s) 261, 1118, 1558
DpnI GATC 8 cut(s) 257, 350, 570, 740, 864, 971, 1448, 1712
DpnII GATC 8 cut(s) 255, 348, 568, 738, 862, 969, 1446, 1710
DrdI GACNNNNNNGTC 1 cut(s) 467
DriI GACNNNNNGTC 1 cut(s) 941
DseDI GACNNNNNNGTC 1 cut(s) 467
EaeI YGGCCR 1 cut(s) 869
Eam1104I CTCTTC 3 cut(s) 331, 1301, 1655
Eam1105I GACNNNNNGTC 1 cut(s) 941
EarI CTCTTC 3 cut(s) 331, 1301, 1655
Ecl136II GAGCTC 1 cut(s) 1390
Eco130I CCWWGG 4 cut(s) 866, 891, 965, 1638
Eco24I GRGCYC 1 cut(s) 1392
Eco31I GGTCTC 2 cut(s) 43, 644
Eco32I GATATC 2 cut(s) 291, 1456
Eco47I GGWCC 3 cut(s) 731, 1020, 1622
Eco53kI GAGCTC 1 cut(s) 1390
Eco57I CTGAAG 2 cut(s) 1208, 1691
Eco81I CCTNAGG 2 cut(s) 1070, 1732
Eco88I CYCGRG 3 cut(s) 164, 264, 1561
EcoICRI GAGCTC 1 cut(s) 1390
EcoNI CCTNNNNNAGG 1 cut(s) 1478
EcoRI GAATTC 1 cut(s) 183
EcoRV GATATC 2 cut(s) 291, 1456
EcoT14I CCWWGG 4 cut(s) 866, 891, 965, 1638
EcoT38I GRGCYC 1 cut(s) 1392
ErhI CCWWGG 4 cut(s) 866, 891, 965, 1638
Esp3I CGTCTC 1 cut(s) 712
FalI AAGNNNNNCTT 2 cut(s) 939, 971
FaqI GGGAC 2 cut(s) 1543, 1628
FblI GTMKAC 1 cut(s) 1470
Fnu4HI GCNGC 6 cut(s) 154, 498, 501, 1110, 1242, 1673
FokI GGATG 8 cut(s) 73, 341, 357, 948, 1250, 1276, 1329, 1496
FriOI GRGCYC 1 cut(s) 1392
Fsp4HI GCNGC 6 cut(s) 154, 498, 501, 1110, 1242, 1673
FspBI CTAG 4 cut(s) 215, 474, 1382, 1418
GlaI GCGC 2 cut(s) 496, 825
GluI GCNGC 6 cut(s) 154, 498, 501, 1110, 1242, 1673
GsaI CCCAGC 1 cut(s) 667
GsuI CTGGAG 1 cut(s) 690
HaeIII GGCC 4 cut(s) 219, 662, 871, 1422
HapII CCGG 4 cut(s) 53, 127, 659, 973
HgaI GACGC 2 cut(s) 422, 1536
HhaI GCGC 2 cut(s) 497, 826
Hin1I GRCGYC 2 cut(s) 1467, 1547
Hin6I GCGC 2 cut(s) 495, 824
HinP1I GCGC 2 cut(s) 495, 824
HincII GTYRAC 1 cut(s) 1471
HindII GTYRAC 1 cut(s) 1471
HindIII AAGCTT 2 cut(s) 396, 1495
HinfI GANTC 6 cut(s) 107, 559, 819, 936, 1000, 1027
HpaII CCGG 4 cut(s) 53, 127, 659, 973
HphI GGTGA 4 cut(s) 179, 188, 236, 461
Hpy166II GTNNAC 3 cut(s) 362, 452, 1471
Hpy188I TCNGA 1 cut(s) 1710
Hpy8I GTNNAC 3 cut(s) 362, 452, 1471
Hpy99I CGWCG 3 cut(s) 22, 1472, 1552
HpyAV CCTTC 4 cut(s) 54, 289, 437, 809
HpyCH4III ACNGT 4 cut(s) 71, 731, 943, 1433
HpyCH4IV ACGT 2 cut(s) 1014, 1467
HpyCH4V TGCA 5 cut(s) 503, 614, 920, 1241, 1333
HpyF10VI GCNNNNNNNGC 3 cut(s) 509, 986, 1387
HpySE526I ACGT 2 cut(s) 1014, 1467
Hsp92I GRCGYC 2 cut(s) 1467, 1547
HspAI GCGC 2 cut(s) 495, 824
Kpn2I TCCGGA 1 cut(s) 972
Kzo9I GATC 8 cut(s) 255, 348, 568, 738, 862, 969, 1446, 1710
LmnI GCTCC 4 cut(s) 709, 1171, 1687, 1702
Lsp1109I GCAGC 4 cut(s) 487, 1121, 1253, 1659
LweI GCATC 6 cut(s) 512, 907, 1218, 1228, 1596, 1669
MaeI CTAG 4 cut(s) 215, 474, 1382, 1418
MaeII ACGT 2 cut(s) 1014, 1467
MaeIII GTNAC 5 cut(s) 235, 1154, 1285, 1433, 1629
MalI GATC 8 cut(s) 257, 350, 570, 740, 864, 971, 1448, 1712
MboI GATC 8 cut(s) 255, 348, 568, 738, 862, 969, 1446, 1710
MflI RGATCY 3 cut(s) 862, 969, 1710
MhlI GDGCHC 2 cut(s) 991, 1392
MlsI TGGCCA 1 cut(s) 871
MluCI AATT 4 cut(s) 183, 227, 724, 1034
MluNI TGGCCA 1 cut(s) 871
MlyI GAGTC 1 cut(s) 930
Mox20I TGGCCA 1 cut(s) 871
MroI TCCGGA 1 cut(s) 972
MroXI GAANNNNTTC 1 cut(s) 1052
MscI TGGCCA 1 cut(s) 871
MseI TTAA 4 cut(s) 390, 573, 903, 1509
MslI CAYNNNNRTG 2 cut(s) 549, 1592
Msp20I TGGCCA 1 cut(s) 871
MspA1I CMGCKG 3 cut(s) 500, 667, 1244
MspI CCGG 4 cut(s) 53, 127, 659, 973
MspR9I CCNGG 1 cut(s) 127
Mva1269I GAATGC 2 cut(s) 1340, 1582
MvnI CGCG 3 cut(s) 156, 495, 824
MwoI GCNNNNNNNGC 3 cut(s) 509, 986, 1387
NciI CCSGG 1 cut(s) 127
NcoI CCATGG 3 cut(s) 866, 965, 1638
NdeII GATC 8 cut(s) 255, 348, 568, 738, 862, 969, 1446, 1710
NlaIV GGNNCC 2 cut(s) 705, 971
NmuCI GTSAC 4 cut(s) 235, 1154, 1285, 1629
NspI RCATGY 1 cut(s) 913
NspV TTCGAA 1 cut(s) 596
PaeR7I CTCGAG 1 cut(s) 1561
PagI TCATGA 3 cut(s) 241, 351, 544
PcsI WCGNNNNNNNCGW 1 cut(s) 1374
PctI GAATGC 2 cut(s) 1340, 1582
PdmI GAANNNNTTC 1 cut(s) 1052
PfeI GAWTC 5 cut(s) 107, 559, 819, 1000, 1027
PflFI GACNNNGTC 1 cut(s) 416
PfoI TCCNGGA 1 cut(s) 125
PinAI ACCGGT 1 cut(s) 52
PkrI GCNGC 6 cut(s) 155, 499, 502, 1111, 1243, 1674
PleI GAGTC 1 cut(s) 930
PpsI GAGTC 1 cut(s) 930
PshAI GACNNNNGTC 1 cut(s) 1620
Psp124BI GAGCTC 1 cut(s) 1392
PspFI CCCAGC 1 cut(s) 663
PspN4I GGNNCC 2 cut(s) 705, 971
PspPI GGNCC 4 cut(s) 661, 731, 1020, 1622
PspXI VCTCGAGB 1 cut(s) 1561
PsuI RGATCY 3 cut(s) 862, 969, 1710
PsyI GACNNNGTC 1 cut(s) 416
RsaI GTAC 3 cut(s) 262, 1119, 1559
RsaNI GTAC 3 cut(s) 261, 1118, 1558
RseI CAYNNNNRTG 2 cut(s) 549, 1592
SacI GAGCTC 1 cut(s) 1392
SalI GTCGAC 1 cut(s) 1469
SaqAI TTAA 4 cut(s) 390, 573, 903, 1509
SatI GCNGC 6 cut(s) 154, 498, 501, 1110, 1242, 1673
Sau3AI GATC 8 cut(s) 255, 348, 568, 738, 862, 969, 1446, 1710
Sau96I GGNCC 4 cut(s) 661, 731, 1020, 1622
ScaI AGTACT 1 cut(s) 1559
SchI GAGTC 1 cut(s) 930
ScrFI CCNGG 1 cut(s) 127
SduI GDGCHC 2 cut(s) 991, 1392
SfaNI GCATC 6 cut(s) 512, 907, 1218, 1228, 1596, 1669
SfcI CTRYAG 1 cut(s) 1650
Sfr274I CTCGAG 1 cut(s) 1561
SfuI TTCGAA 1 cut(s) 596
SinI GGWCC 3 cut(s) 731, 1020, 1622
SlaI CTCGAG 1 cut(s) 1561
SmiMI CAYNNNNRTG 2 cut(s) 549, 1592
SmlI CTYRAG 3 cut(s) 191, 1391, 1561
SmoI CTYRAG 3 cut(s) 191, 1391, 1561
Sse9I AATT 4 cut(s) 183, 227, 724, 1034
SsiI CCGC 6 cut(s) 154, 463, 498, 667, 1082, 1244
SspI AATATT 1 cut(s) 379
SspMI CTAG 4 cut(s) 215, 474, 1382, 1418
SstI GAGCTC 1 cut(s) 1392
StyD4I CCNGG 1 cut(s) 125
StyI CCWWGG 4 cut(s) 866, 891, 965, 1638
TaaI ACNGT 4 cut(s) 71, 731, 943, 1433
TaiI ACGT 2 cut(s) 1017, 1470
TasI AATT 4 cut(s) 183, 227, 724, 1034
TatI WGTACW 2 cut(s) 1117, 1557
TauI GCSGC 2 cut(s) 156, 500
TfiI GAWTC 5 cut(s) 107, 559, 819, 1000, 1027
Tru1I TTAA 4 cut(s) 390, 573, 903, 1509
Tru9I TTAA 4 cut(s) 390, 573, 903, 1509
TscAI CASTG 3 cut(s) 493, 1147, 1448
TseFI GTSAC 4 cut(s) 235, 1154, 1285, 1629
TseI GCWGC 4 cut(s) 500, 1109, 1241, 1672
Tsp45I GTSAC 4 cut(s) 235, 1154, 1285, 1629
TspDTI ATGAA 6 cut(s) 258, 551, 561, 900, 1061, 1535
TspGWI ACGGA 4 cut(s) 326, 1383, 1504, 1547
TspRI CASTG 3 cut(s) 493, 1147, 1448
Tth111I GACNNNGTC 1 cut(s) 416
VpaK11BI GGWCC 3 cut(s) 731, 1020, 1622
XagI CCTNNNNNAGG 1 cut(s) 1478
XapI RAATTY 2 cut(s) 183, 227
XbaI TCTAGA 1 cut(s) 473
XceI RCATGY 1 cut(s) 913
XhoI CTCGAG 1 cut(s) 1561
XmiI GTMKAC 1 cut(s) 1470
XmnI GAANNNNTTC 1 cut(s) 1052
XspI CTAG 4 cut(s) 215, 474, 1382, 1418
ZraI GACGTC 1 cut(s) 1468
ZrmI AGTACT 1 cut(s) 1559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.