Rh4AG142500

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0017037
Physical Location & Seq
Reverse (-)
36141669 .. 36144908
3240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG142500.1

Sequence Viewer

Length: 1575 bp
ATGGGAAGTTGCGTTTGTACACCTGCCAAAGCAGGTTTACTACACAAAAAACACAGAGATCAGGTGATCAAGCCTATACCCCAATGCGTTCATGACTCGCCTCAAAAGTCGTCGAACCACCGCGGGGGCTTCTCGGCCCCCATCTCGGTGCTGCACAAGACGGATGGAGAAAACATCCTTGACAAGTACAAGTTGGGCAAGGAGTTGGGGCGAGGGGAGTTTGGAATCACCCACCGATGCGTGGACCGGCAGACCGGCGAGACCTACGCGTGCAAGGTGATATCAAAGAGCAAGTTGAAGACAGAGATTGACATTGAAGACGTGAGGAGGGAGGCCGAGATCATGAGGCTTTTGCCCAAGCACCCCAACATCGTCACCTTCAAGGAGGCTTATGAGGACAAGGATGCAGTTTATCTTGTCATGGAGCTCTGTGAGGGAGGCGAGCTTTTCGATAGGATCGTCACAAAAGGCCACTACACGGAGCGCGCCGCCGCCCACGTTACCAAGACAATTCTCGAGGTTTGCAAGGTATGCCACGAGCATGGAGTGATTCATCGAGACCTGAAACCCGAGAATTTCCTTTTTGCAGATAGTAGTGAAAATGCACAGTTAAAGGCAATTGATTTTGGTCTATCAGTAAATTATGAACCAGGTCAGAGATTCTACGAAATTGTTGGAAGTCCATATTACATGGCTCCAGAGGTATTACGACGTAATTATGGGCCTGAAGTTGATGTTTGGAGCGCAGGAGTTATCCTCTATATCCTACTTTGTGGAGTTCCTCCATTTTGGGCAGAAACTGAAGAAGGGATTGCACAGGCTATTATTCGGGGTAACATTGATTTTCAGAAGGATACGTGGAAAAAGGTTTCTGAGGAAGCAAAATTACTAGTGAGGAGTATGCTTGACCCAAATCCTTACAACAGACTCACAGTTGAAGAAGTCCTTGATCATCCCTGGATACGAAATGCAAATCAAGTTCCGAATGTTCCTCTGGCAGAAAATGTCAGATTAAGGATCAAGCAGTTCACATTGATGAATAAATTTAAGAAGAAAGTTCTCAGAGTGGTGGCAGACAACCTGCCAGATGAACAAATAGAAGGAATCAGACAGATATTTGATATGATGGACACCGATAACAATGGAGACTTGACATTTGAAGAGCTTAGGGATGGTCTTCTCAAGATTGGACACCAAGTCACTGATCCTGATGTGCAAATGTTAATTGATGCCGCGGATGTCGATGGAAGTGGCACACTGAACTGTGAGGAGTTCGTTATTGTGTCTGTTCACTTGAAAAAAATAAGCAGCGACGAACTTCTCGCTCAAGCTTTTAGCTATTTTGACAAGAACAACAGCAACTATATTGAATTTGATGAGTTAAGAGAAGCATTGCTGGATGATAAACTTGGTCCTACTAATGAGCAGGTTATCCAAGACATCATATTCGATGTGGACTTGGACAAGGTAATTCTCAAACACAATTCACCAATTTCCCCTCCTATATGCTGTTCTGAGTACTATTATAGGCAGTTTATTTTTCGGATACAACTAGGATATTTTCGGATAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010769 GO:0010857 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043269 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0046777 GO:0047484 GO:0048519 GO:0048583 GO:0048585 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080092 GO:0080134 GO:0097305 GO:0097306 GO:0140096 GO:1901000 GO:1901001 GO:1901016 GO:1901379 GO:1901564 GO:1901700 GO:1901701 GO:1901979 GO:1904062 GO:2000241 GO:2001257
KEGG Pathways
Metabolic & Signaling
ko04626 ko05145 map04626 map05145
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

524

Amino Acids

59.79

Weight (kDa)

5.49

Isoelectric Point (pI)

36.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 63 - 321 2.4e-79 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 64 - 316 1.9e-28 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 135 - 220 7.3e-06 ABC1 atypical kinase-like domain
EF-hand_7 PF13499 367 - 426 2e-10 EF-hand domain pair
EF-hand_6 PF13405 369 - 397 7e-06 EF-hand domain
EF-hand_1 PF00036 369 - 393 9e-06 EF hand domain
EF-hand_8 PF13833 381 - 431 7e-08 EF-hand domain pair
EF-hand_7 PF13499 441 - 489 8.8e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31500
fragaria_vesca FvH4_4g11140
malus_domestica MD09G1092200.v1.1 MD17G1082100.v1.1
prunus_persica Prupe.1G190700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0407071
rosa_laevigata RLG00000008739
rosa_multiflora Rmu_sc0000600.1_g000002
rosa_rugosa Rorug04G0077800
rosa_samantha Rh4AG142500 Rh4CG149000 Rh4DG136600
rosa_wichuraiana Rw4G011610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 4 cut(s) 23, 31, 1089, 1417
AccII CGCG 4 cut(s) 123, 269, 486, 1235
AciI CCGC 6 cut(s) 121, 123, 489, 492, 1233, 1235
AclWI GGATC 3 cut(s) 464, 1025, 1199
AcsI RAATTY 3 cut(s) 574, 1043, 1370
AcuI CTGAAG 2 cut(s) 747, 822
AfaI GTAC 3 cut(s) 19, 188, 1520
AfiI CCNNNNNNNGG 4 cut(s) 124, 145, 241, 478
AflIII ACRYGT 1 cut(s) 267
AgsI TTSAA 7 cut(s) 298, 317, 382, 938, 1160, 1297, 1370
AhdI GACNNNNNGTC 1 cut(s) 1196
AhlI ACTAGT 1 cut(s) 889
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 2 cut(s) 649, 956
AluBI AGCT 5 cut(s) 427, 445, 1165, 1331, 1338
AluI AGCT 5 cut(s) 427, 445, 1165, 1331, 1338
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 3 cut(s) 254, 552, 1140
AlwI GGATC 3 cut(s) 464, 1025, 1199
AlwNI CAGNNNCTG 1 cut(s) 800
Ama87I CYCGRG 2 cut(s) 515, 569
AoxI GGCC 4 cut(s) 135, 333, 469, 722
ApeKI GCWGC 2 cut(s) 151, 1308
ApoI RAATTY 3 cut(s) 574, 1043, 1370
ArsI GACNNNNNNTTYG 4 cut(s) 1139, 1171, 1430, 1462
AspLEI GCGC 3 cut(s) 486, 488, 746
AspS9I GGNCC 4 cut(s) 136, 244, 722, 1412
AsuHPI GGTGA 5 cut(s) 76, 220, 289, 367, 1479
AvaI CYCGRG 2 cut(s) 515, 569
AvaII GGWCC 2 cut(s) 244, 1412
BanII GRGCYC 1 cut(s) 429
BarI GAAGNNNNNNTAC 2 cut(s) 870, 902
BauI CACGAG 1 cut(s) 536
BbsI GAAGAC 3 cut(s) 305, 324, 1169
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 2 cut(s) 138, 1320
BccI CCATC 5 cut(s) 149, 158, 1120, 1166, 1238
BciT130I CCWGG 2 cut(s) 651, 958
BciVI GTATCC 3 cut(s) 847, 954, 1539
BclI TGATCA 2 cut(s) 66, 949
BcoDI GTCTC 3 cut(s) 254, 552, 1140
BcuI ACTAGT 1 cut(s) 889
BfaI CTAG 2 cut(s) 890, 1553
BfuAI ACCTGC 4 cut(s) 23, 31, 1089, 1417
BfuI GTATCC 3 cut(s) 847, 954, 1539
BisI GCNGC 5 cut(s) 152, 489, 492, 1233, 1309
BlsI GCNGC 5 cut(s) 153, 490, 493, 1234, 1310
BmcAI AGTACT 1 cut(s) 1520
Bme1390I CCNGG 2 cut(s) 651, 958
Bme18I GGWCC 2 cut(s) 244, 1412
BmeRI GACNNNNNGTC 1 cut(s) 1196
BmeT110I CYCGRG 2 cut(s) 515, 569
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 4 cut(s) 136, 244, 722, 1412
BmiI GGNNCC 2 cut(s) 138, 696
BmrFI CCNGG 2 cut(s) 651, 958
BmsI GCATC 3 cut(s) 227, 394, 1219
BpiI GAAGAC 3 cut(s) 305, 324, 1169
BplI GAGNNNNNCTC 2 cut(s) 741, 773
BpmI CTGGAG 1 cut(s) 681
Bpu10I CCTNAGC 1 cut(s) 1166
BpuEI CTTGAG 2 cut(s) 1166, 1311
BsaAI YACGTR 1 cut(s) 858
BsaI GGTCTC 2 cut(s) 254, 552
BsaJI CCNNGG 3 cut(s) 121, 956, 1233
Bsc4I CCNNNNNNNGG 4 cut(s) 124, 145, 241, 478
Bse118I RCCGGY 2 cut(s) 246, 254
Bse3DI GCAATG 1 cut(s) 1391
BseBI CCWGG 2 cut(s) 651, 958
BseDI CCNNGG 3 cut(s) 121, 956, 1233
BseGI GGATG 7 cut(s) 169, 174, 409, 952, 1177, 1243, 1405
BseLI CCNNNNNNNGG 4 cut(s) 124, 145, 241, 478
BseMI GCAATG 1 cut(s) 1391
BseMII CTCAG 3 cut(s) 864, 1075, 1506
BsePI GCGCGC 1 cut(s) 484
BseRI GAGGAG 3 cut(s) 340, 910, 1283
BseXI GCAGC 2 cut(s) 138, 1320
BsgI GTGCAG 1 cut(s) 137
Bsh1236I CGCG 4 cut(s) 123, 269, 486, 1235
BshFI GGCC 4 cut(s) 137, 335, 471, 724
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 2 cut(s) 515, 569
BsiSI CCGG 2 cut(s) 247, 255
BslI CCNNNNNNNGG 4 cut(s) 124, 145, 241, 478
BsmAI GTCTC 3 cut(s) 254, 552, 1140
BsnI GGCC 4 cut(s) 137, 335, 471, 724
Bso31I GGTCTC 2 cut(s) 254, 552
BsoBI CYCGRG 2 cut(s) 515, 569
Bsp1286I GDGCHC 1 cut(s) 429
Bsp1407I TGTACA 1 cut(s) 17
Bsp143I GATC 7 cut(s) 58, 66, 339, 456, 949, 1017, 1204
BspACI CCGC 6 cut(s) 121, 123, 489, 492, 1233, 1235
BspANI GGCC 4 cut(s) 137, 335, 471, 724
BspCNI CTCAG 3 cut(s) 865, 1074, 1507
BspFNI CGCG 4 cut(s) 123, 269, 486, 1235
BspHI TCATGA 2 cut(s) 91, 342
BspLI GGNNCC 2 cut(s) 138, 696
BspMI ACCTGC 4 cut(s) 23, 31, 1089, 1417
BspPI GGATC 3 cut(s) 464, 1025, 1199
BspQI GCTCTTC 1 cut(s) 1155
BspTNI GGTCTC 2 cut(s) 254, 552
BsrDI GCAATG 1 cut(s) 1391
BsrFI RCCGGY 2 cut(s) 246, 254
BsrGI TGTACA 1 cut(s) 17
BssAI RCCGGY 2 cut(s) 246, 254
BssECI CCNNGG 3 cut(s) 121, 956, 1233
BssHII GCGCGC 1 cut(s) 484
BssMI GATC 7 cut(s) 58, 66, 339, 456, 949, 1017, 1204
BssSI CACGAG 1 cut(s) 536
Bst2BI CACGAG 1 cut(s) 536
Bst2UI CCWGG 2 cut(s) 651, 958
Bst4CI ACNGT 3 cut(s) 609, 934, 1265
Bst6I CTCTTC 1 cut(s) 1155
BstAPI GCANNNNNTGC 1 cut(s) 531
BstAUI TGTACA 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 858
BstC8I GCNNGC 3 cut(s) 271, 443, 486
BstDEI CTNAG 4 cut(s) 873, 1061, 1166, 1515
BstDSI CCRYGG 2 cut(s) 121, 1233
BstF5I GGATG 7 cut(s) 169, 174, 409, 952, 1177, 1243, 1405
BstFNI CGCG 4 cut(s) 123, 269, 486, 1235
BstHHI GCGC 3 cut(s) 486, 488, 746
BstKTI GATC 7 cut(s) 61, 69, 342, 459, 952, 1020, 1207
BstMAI GTCTC 3 cut(s) 254, 552, 1140
BstMBI GATC 7 cut(s) 58, 66, 339, 456, 949, 1017, 1204
BstMWI GCNNNNNNNGC 1 cut(s) 531
BstNI CCWGG 2 cut(s) 651, 958
BstSCI CCNGG 2 cut(s) 649, 956
BstUI CGCG 4 cut(s) 123, 269, 486, 1235
BstV1I GCAGC 2 cut(s) 138, 1320
BstV2I GAAGAC 3 cut(s) 305, 324, 1169
BstXI CCANNNNNNTGG 1 cut(s) 542
BsuI GTATCC 3 cut(s) 847, 954, 1539
BsuRI GGCC 4 cut(s) 137, 335, 471, 724
BtgI CCRYGG 2 cut(s) 121, 1233
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 7 cut(s) 169, 174, 409, 952, 1177, 1243, 1405
BtsIMutI CAGTG 2 cut(s) 1200, 1256
BveI ACCTGC 4 cut(s) 23, 31, 1089, 1417
Cac8I GCNNGC 3 cut(s) 271, 443, 486
CaiI CAGNNNCTG 1 cut(s) 800
CciI TCATGA 2 cut(s) 91, 342
CfoI GCGC 3 cut(s) 486, 488, 746
Cfr10I RCCGGY 2 cut(s) 246, 254
Cfr13I GGNCC 4 cut(s) 136, 244, 722, 1412
Cfr42I CCGCGG 2 cut(s) 124, 1236
CsiI ACCWGGT 1 cut(s) 649
Csp6I GTAC 3 cut(s) 18, 187, 1519
CviAII CATG 5 cut(s) 92, 343, 421, 542, 691
CviQI GTAC 3 cut(s) 18, 187, 1519
DdeI CTNAG 4 cut(s) 873, 1061, 1166, 1515
DpnI GATC 7 cut(s) 60, 68, 341, 458, 951, 1019, 1206
DpnII GATC 7 cut(s) 58, 66, 339, 456, 949, 1017, 1204
DriI GACNNNNNGTC 1 cut(s) 1196
Eam1104I CTCTTC 1 cut(s) 1155
Eam1105I GACNNNNNGTC 1 cut(s) 1196
EarI CTCTTC 1 cut(s) 1155
Ecl136II GAGCTC 1 cut(s) 427
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 2 cut(s) 254, 552
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 2 cut(s) 244, 1412
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 2 cut(s) 747, 822
Eco88I CYCGRG 2 cut(s) 515, 569
EcoICRI GAGCTC 1 cut(s) 427
EcoRII CCWGG 2 cut(s) 649, 956
EcoRV GATATC 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 429
FaeI CATG 5 cut(s) 95, 346, 424, 545, 694
FalI AAGNNNNNCTT 2 cut(s) 930, 962
FatI CATG 5 cut(s) 91, 342, 420, 541, 690
FauI CCCGC 1 cut(s) 116
FbaI TGATCA 2 cut(s) 66, 949
Fnu4HI GCNGC 5 cut(s) 152, 489, 492, 1233, 1309
FokI GGATG 7 cut(s) 161, 176, 416, 939, 1184, 1250, 1412
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 5 cut(s) 152, 489, 492, 1233, 1309
FspBI CTAG 2 cut(s) 890, 1553
GlaI GCGC 3 cut(s) 485, 487, 745
GluI GCNGC 5 cut(s) 152, 489, 492, 1233, 1309
GsuI CTGGAG 1 cut(s) 681
HaeIII GGCC 4 cut(s) 137, 335, 471, 724
HapII CCGG 2 cut(s) 247, 255
HhaI GCGC 3 cut(s) 486, 488, 746
Hin1II CATG 5 cut(s) 95, 346, 424, 545, 694
Hin6I GCGC 3 cut(s) 484, 486, 744
HinP1I GCGC 3 cut(s) 484, 486, 744
HindIII AAGCTT 1 cut(s) 1329
HinfI GANTC 6 cut(s) 95, 225, 550, 660, 927, 1104
HpaII CCGG 2 cut(s) 247, 255
HphI GGTGA 5 cut(s) 76, 220, 289, 367, 1479
Hpy166II GTNNAC 6 cut(s) 20, 38, 244, 1029, 1291, 1456
Hpy188III TCNNGA 7 cut(s) 92, 343, 515, 557, 698, 1183, 1208
Hpy8I GTNNAC 6 cut(s) 20, 38, 244, 1029, 1291, 1456
Hpy99I CGWCG 3 cut(s) 115, 714, 1316
HpyAV CCTTC 4 cut(s) 388, 800, 844, 1094
HpyCH4III ACNGT 3 cut(s) 609, 934, 1265
HpyCH4IV ACGT 4 cut(s) 321, 498, 712, 857
HpyCH4V TGCA 9 cut(s) 154, 273, 407, 525, 587, 605, 815, 971, 1216
HpyF10VI GCNNNNNNNGC 1 cut(s) 531
HpyF3I CTNAG 4 cut(s) 873, 1061, 1166, 1515
HpySE526I ACGT 4 cut(s) 321, 498, 712, 857
Hsp92II CATG 5 cut(s) 95, 346, 424, 545, 694
HspAI GCGC 3 cut(s) 484, 486, 744
Ksp22I TGATCA 2 cut(s) 66, 949
KspI CCGCGG 2 cut(s) 124, 1236
Kzo9I GATC 7 cut(s) 58, 66, 339, 456, 949, 1017, 1204
LguI GCTCTTC 1 cut(s) 1155
LmnI GCTCC 4 cut(s) 424, 481, 700, 741
Lsp1109I GCAGC 2 cut(s) 138, 1320
LweI GCATC 3 cut(s) 227, 394, 1219
MabI ACCWGGT 1 cut(s) 649
MaeI CTAG 2 cut(s) 890, 1553
MaeII ACGT 4 cut(s) 321, 498, 712, 857
MaeIII GTNAC 5 cut(s) 373, 460, 499, 833, 1198
MalI GATC 7 cut(s) 60, 68, 341, 458, 951, 1019, 1206
MboI GATC 7 cut(s) 58, 66, 339, 456, 949, 1017, 1204
MboII GAAGA 7 cut(s) 310, 329, 815, 950, 1063, 1169, 1172
MfeI CAATTG 1 cut(s) 618
MhlI GDGCHC 1 cut(s) 429
MluI ACGCGT 1 cut(s) 267
MlyI GAGTC 2 cut(s) 89, 921
MmeI TCCRAC 1 cut(s) 655
MseI TTAA 6 cut(s) 611, 1013, 1047, 1223, 1382, 1573
MslI CAYNNNNRTG 3 cut(s) 146, 540, 1034
MspA1I CMGCKG 2 cut(s) 123, 1235
MspI CCGG 2 cut(s) 247, 255
MspR9I CCNGG 2 cut(s) 651, 958
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 2 cut(s) 651, 958
MvnI CGCG 4 cut(s) 123, 269, 486, 1235
MwoI GCNNNNNNNGC 1 cut(s) 531
NdeII GATC 7 cut(s) 58, 66, 339, 456, 949, 1017, 1204
NlaIII CATG 5 cut(s) 95, 346, 424, 545, 694
NlaIV GGNNCC 2 cut(s) 138, 696
NmeAIII GCCGAG 2 cut(s) 113, 361
NmuCI GTSAC 3 cut(s) 373, 460, 1198
PaeR7I CTCGAG 1 cut(s) 515
PagI TCATGA 2 cut(s) 91, 342
PaqCI CACCTGC 1 cut(s) 31
PauI GCGCGC 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 1155
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PfeI GAWTC 4 cut(s) 225, 550, 660, 1104
PkrI GCNGC 5 cut(s) 153, 490, 493, 1234, 1310
PleI GAGTC 2 cut(s) 89, 921
PpsI GAGTC 2 cut(s) 89, 921
Ppu21I YACGTR 1 cut(s) 858
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 649, 956
PspGI CCWGG 2 cut(s) 649, 956
PspN4I GGNNCC 2 cut(s) 138, 696
PspPI GGNCC 4 cut(s) 136, 244, 722, 1412
PstNI CAGNNNCTG 1 cut(s) 800
PteI GCGCGC 1 cut(s) 484
RsaI GTAC 3 cut(s) 19, 188, 1520
RsaNI GTAC 3 cut(s) 18, 187, 1519
RseI CAYNNNNRTG 3 cut(s) 146, 540, 1034
SacI GAGCTC 1 cut(s) 429
SacII CCGCGG 2 cut(s) 124, 1236
SapI GCTCTTC 1 cut(s) 1155
SaqAI TTAA 6 cut(s) 611, 1013, 1047, 1223, 1382, 1573
SatI GCNGC 5 cut(s) 152, 489, 492, 1233, 1309
Sau3AI GATC 7 cut(s) 58, 66, 339, 456, 949, 1017, 1204
Sau96I GGNCC 4 cut(s) 136, 244, 722, 1412
ScaI AGTACT 1 cut(s) 1520
SchI GAGTC 2 cut(s) 89, 921
ScrFI CCNGG 2 cut(s) 651, 958
SduI GDGCHC 1 cut(s) 429
SexAI ACCWGGT 1 cut(s) 649
SfaNI GCATC 3 cut(s) 227, 394, 1219
Sfr274I CTCGAG 1 cut(s) 515
Sfr303I CCGCGG 2 cut(s) 124, 1236
SgrBI CCGCGG 2 cut(s) 124, 1236
SinI GGWCC 2 cut(s) 244, 1412
SlaI CTCGAG 1 cut(s) 515
SmiMI CAYNNNNRTG 3 cut(s) 146, 540, 1034
SmlI CTYRAG 3 cut(s) 515, 1181, 1326
SmoI CTYRAG 3 cut(s) 515, 1181, 1326
SpeI ACTAGT 1 cut(s) 889
SsiI CCGC 6 cut(s) 121, 123, 489, 492, 1233, 1235
SspMI CTAG 2 cut(s) 890, 1553
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 2 cut(s) 649, 956
TaaI ACNGT 3 cut(s) 609, 934, 1265
TaiI ACGT 4 cut(s) 324, 501, 715, 860
TaqI TCGA 6 cut(s) 113, 450, 516, 556, 1242, 1449
TatI WGTACW 3 cut(s) 17, 186, 1518
TauI GCSGC 3 cut(s) 491, 494, 1235
TfiI GAWTC 4 cut(s) 225, 550, 660, 1104
Tru1I TTAA 6 cut(s) 611, 1013, 1047, 1223, 1382, 1573
Tru9I TTAA 6 cut(s) 611, 1013, 1047, 1223, 1382, 1573
TscAI CASTG 2 cut(s) 1207, 1263
TseFI GTSAC 3 cut(s) 373, 460, 1198
TseI GCWGC 2 cut(s) 151, 1308
Tsp45I GTSAC 3 cut(s) 373, 460, 1198
TspDTI ATGAA 5 cut(s) 80, 542, 660, 1052, 1104
TspGWI ACGGA 2 cut(s) 176, 494
TspRI CASTG 2 cut(s) 1207, 1263
VpaK11BI GGWCC 2 cut(s) 244, 1412
XapI RAATTY 3 cut(s) 574, 1043, 1370
XhoI CTCGAG 1 cut(s) 515
XspI CTAG 2 cut(s) 890, 1553
ZrmI AGTACT 1 cut(s) 1520
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.