MD09G1092200.v1.1

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0017037
Physical Location & Seq
Forward (+)
6775357 .. 6778287
2931 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1092200.v1.1.491

Sequence Viewer

Length: 1614 bp
ATGGGAAGCTGCGTATGCACACCTGCGAAAGCAGGTTTCCACCACAGACGAAGAGACATCATCAAACCTTTACCTCAAAGCAGTGTACACGACTCGTCGCCACGCAGGTCGTTCGTTGCACCGGTTCCCCACCACAGCACTATTATATGGGTGCTGGACAAGACTGCCGGTAATGACATCTTCGGCAAGTACCAGTTTGGCAATGAATTAGGGCGAGGGGAGTTTGGAATCACCTACAGATGCGTCCACAGGGAGACGGGGGAGGCTTTTGCGTGCAAGACCATATCGAAGAGCAAGCTGAAGACGGAGATTGATGTCGAGGATGTGAGGAGGGAGGCTGAGATCATGCGCCGTTTGCCTAAGCATCCGAATATTGTCTGCTTCAAGGAGGCTTATGAGGATAGGGAGGCAGTTTATCTTGTCATGGAGCTTTGCGAAGGCGGCGAGCTCTTTGATAGGATTGTGGCAAAAGGGCATTACACGGAGCGAGCTGCCGCCACTGTTACCAGGACAATACTTGAGATTTGCCAGGTATGCCACAAGCATGGAGTGATACATAGGGACCTGAAACCAGAGAATTTCCTTTTTGCAGATGGTAGTGAAAATGCAGAATTAAAGGCAATTGATTTCGGCCTCTCTATATTTTTCGAACCAGGTCAGAGATTCAACGAAATTGTTGGAAGTCCATATTACATGGCTCCGGAGGTGCTACGGCGTAATTATGGGCCGGAAGTTGATGTTTGGAGTTCAGGGGTTATCCTTTACATCCTACTTTGTGGAGTGCCTCCATTTTGGGCAGAAACTGAGGAAGGAATTGCACAGGCTATTGTTCGGGGTACAATAGATTTCGAGAGGGAGACATGGAAAAATGTTTCGGAGGAAGCAAAAGAACTAGTGAGGAGCATGCTTGATCCAAATCCTTGCAACAGGCTCAAAGTTGAAGAAGTCCTAGATCATCCGTGGATAAGAAACGGGAATCAAGTTCCTAATGTTCCTCTGGGAGAAAATGTCACATTAAGGATCAAGCAGTTCTCGTTAATGAATAAGTTCAAGAAGAAAGTTCTTAGAGTGGTGGCGGATAACTTGCCAGAGGATCAAATAGATGGAATCAGACAGATATTCGAAATGATGGACATTGACAACAACGGAAACTTGACTTTTGAAGAACTTCGAGATGGTCTTGTGAAGATCGGACACAATGTCGCTGATCCTGATGTGCAGATGTTGTTGGATGCTGCGGATGCTGATGGAAATGGCACACTGAGCTGCGATGAATTCGTTATGGTGTCTGTTCACTTAAAAAAGATAGACAGCGATGAATTTCTCCATCAAGCTTTTGGCTATTTCGACAAGAATAACAGCGGATATATTGAGTTCGATGAGCTGAGAGAAGCATTGCTGGATGACAAACTTGGTCCAGATAACGAGCAGGTTATCCAAGACATCATATTTGATGTTGACTTGGACAAGGATGGGCGAATAGGTTTTGAAGAGTTTAAAGCAATGATGAAAACTGGCATGGACTGGAAAATGGCTTCTCGTCAATATTCGAGAGCATTGCTAAATGTACTTAGCCGTAAGTTGCTGAAAGACGAATCGTTACAAGCGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010769 GO:0010857 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043269 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0046777 GO:0047484 GO:0048519 GO:0048583 GO:0048585 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080092 GO:0080134 GO:0097305 GO:0097306 GO:0140096 GO:1901000 GO:1901001 GO:1901016 GO:1901379 GO:1901564 GO:1901700 GO:1901701 GO:1901979 GO:1904062 GO:2000241 GO:2001257
KEGG Pathways
Metabolic & Signaling
ko04626 ko05145 map04626 map05145
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

538

Amino Acids

61.13

Weight (kDa)

5.27

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 64 - 322 4.1e-78 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 65 - 317 1.2e-28 Protein tyrosine and serine/threonine kinase
EF-hand_7 PF13499 369 - 427 1.1e-09 EF-hand domain pair
EF-hand_1 PF00036 370 - 396 5.2e-06 EF hand domain
EF-hand_8 PF13833 382 - 432 3e-07 EF-hand domain pair
EF-hand_7 PF13499 440 - 504 2.2e-12 EF-hand domain pair
EF-hand_1 PF00036 442 - 466 4.2e-06 EF hand domain
EF-hand_6 PF13405 442 - 467 5.3e-06 EF-hand domain
EF-hand_1 PF00036 479 - 505 6.5e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31500
fragaria_vesca FvH4_4g11140
malus_domestica MD09G1092200.v1.1 MD17G1082100.v1.1
prunus_persica Prupe.1G190700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0407071
rosa_laevigata RLG00000008739
rosa_multiflora Rmu_sc0000600.1_g000002
rosa_rugosa Rorug04G0077800
rosa_samantha Rh4AG142500 Rh4CG149000 Rh4DG136600
rosa_wichuraiana Rw4G011610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 4 cut(s) 23, 31, 96, 1420
AccIII TCCGGA 1 cut(s) 700
AciI CCGC 5 cut(s) 441, 495, 1076, 1238, 1362
AclWI GGATC 4 cut(s) 905, 1028, 1101, 1202
AcsI RAATTY 3 cut(s) 577, 1274, 1319
AcuI CTGAAG 1 cut(s) 320
AfaI GTAC 4 cut(s) 87, 191, 838, 1569
AgeI ACCGGT 1 cut(s) 121
AgsI TTSAA 6 cut(s) 385, 667, 941, 1051, 1163, 1490
AhdI GACNNNNNGTC 1 cut(s) 1199
AhlI ACTAGT 1 cut(s) 892
AjnI CCWGG 3 cut(s) 506, 528, 652
AluBI AGCT 8 cut(s) 9, 298, 430, 448, 491, 1266, 1334, 1384
AluI AGCT 8 cut(s) 9, 298, 430, 448, 491, 1266, 1334, 1384
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 3 cut(s) 48, 248, 851
AlwI GGATC 4 cut(s) 905, 1028, 1101, 1202
AlwNI CAGNNNCTG 1 cut(s) 803
Aor13HI TCCGGA 1 cut(s) 700
AoxI GGCC 2 cut(s) 631, 725
ApeKI GCWGC 4 cut(s) 9, 491, 1235, 1266
ApoI RAATTY 3 cut(s) 577, 1274, 1319
ArsI GACNNNNNNTTYG 2 cut(s) 1433, 1465
AsiGI ACCGGT 1 cut(s) 121
Asp700I GAANNNNTTC 2 cut(s) 1046, 1167
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 3 cut(s) 562, 725, 1415
AsuHPI GGTGA 1 cut(s) 223
AsuII TTCGAA 2 cut(s) 648, 1122
AvaII GGWCC 2 cut(s) 562, 1415
BanII GRGCYC 1 cut(s) 450
BbsI GAAGAC 1 cut(s) 308
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 3 cut(s) 478, 1222, 1253
BccI CCATC 7 cut(s) 587, 1097, 1123, 1169, 1241, 1335, 1466
BceAI ACGGC 3 cut(s) 336, 728, 1560
BcgI CGANNNNNNTGC 4 cut(s) 94, 128, 1540, 1574
BciT130I CCWGG 3 cut(s) 508, 530, 654
BcoDI GTCTC 3 cut(s) 48, 248, 851
BcuI ACTAGT 1 cut(s) 892
BfaI CTAG 2 cut(s) 893, 950
BfmI CTRYAG 1 cut(s) 235
BfuAI ACCTGC 4 cut(s) 23, 31, 96, 1420
BisI GCNGC 6 cut(s) 10, 442, 492, 495, 1236, 1267
BlsI GCNGC 6 cut(s) 11, 443, 493, 496, 1237, 1268
Bme1390I CCNGG 3 cut(s) 508, 530, 654
Bme18I GGWCC 2 cut(s) 562, 1415
BmeRI GACNNNNNGTC 1 cut(s) 1199
BmgT120I GGNCC 3 cut(s) 562, 725, 1415
BmiI GGNNCC 3 cut(s) 126, 563, 699
BmrFI CCNGG 3 cut(s) 508, 530, 654
BmsI GCATC 4 cut(s) 230, 373, 1222, 1231
BpiI GAAGAC 1 cut(s) 308
Bpu10I CCTNAGC 1 cut(s) 360
Bpu14I TTCGAA 2 cut(s) 648, 1122
BpuEI CTTGAG 1 cut(s) 539
BsaBI GATNNNNATC 1 cut(s) 915
BsaJI CCNNGG 1 cut(s) 959
BsaWI WCCGGW 2 cut(s) 121, 700
BsaXI ACNNNNNCTCC 2 cut(s) 993, 1023
Bse118I RCCGGY 2 cut(s) 121, 167
Bse1I ACTGG 3 cut(s) 193, 1519, 1529
Bse3DI GCAATG 4 cut(s) 208, 1394, 1509, 1556
Bse8I GATNNNNATC 1 cut(s) 915
BseAI TCCGGA 1 cut(s) 700
BseBI CCWGG 3 cut(s) 508, 530, 654
BseDI CCNNGG 1 cut(s) 959
BseGI GGATG 8 cut(s) 328, 364, 765, 955, 1237, 1246, 1408, 1477
BseJI GATNNNNATC 1 cut(s) 915
BseMI GCAATG 4 cut(s) 208, 1394, 1509, 1556
BseMII CTCAG 4 cut(s) 330, 795, 1253, 1376
BseNI ACTGG 3 cut(s) 193, 1519, 1529
BseRI GAGGAG 2 cut(s) 343, 913
BseXI GCAGC 3 cut(s) 478, 1222, 1253
BsgI GTGCAG 1 cut(s) 1238
BshFI GGCC 2 cut(s) 633, 727
BshTI ACCGGT 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 450
BsiSI CCGG 4 cut(s) 122, 168, 701, 728
BslFI GGGAC 1 cut(s) 575
BsmAI GTCTC 3 cut(s) 48, 248, 851
BsmBI CGTCTC 1 cut(s) 248
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 2 cut(s) 633, 727
Bsp119I TTCGAA 2 cut(s) 648, 1122
Bsp1286I GDGCHC 1 cut(s) 450
Bsp13I TCCGGA 1 cut(s) 700
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 7 cut(s) 342, 910, 952, 1020, 1093, 1188, 1207
BspACI CCGC 5 cut(s) 441, 495, 1076, 1238, 1362
BspANI GGCC 2 cut(s) 633, 727
BspCNI CTCAG 4 cut(s) 331, 796, 1254, 1377
BspEI TCCGGA 1 cut(s) 700
BspLI GGNNCC 3 cut(s) 126, 563, 699
BspMI ACCTGC 4 cut(s) 23, 31, 96, 1420
BspPI GGATC 4 cut(s) 905, 1028, 1101, 1202
BspQI GCTCTTC 1 cut(s) 284
BspT104I TTCGAA 2 cut(s) 648, 1122
BsrDI GCAATG 4 cut(s) 208, 1394, 1509, 1556
BsrFI RCCGGY 2 cut(s) 121, 167
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 3 cut(s) 193, 1519, 1529
BssAI RCCGGY 2 cut(s) 121, 167
BssECI CCNNGG 1 cut(s) 959
BssMI GATC 7 cut(s) 342, 910, 952, 1020, 1093, 1188, 1207
Bst2UI CCWGG 3 cut(s) 508, 530, 654
Bst4CI ACNGT 1 cut(s) 502
Bst6I CTCTTC 3 cut(s) 46, 284, 1485
BstAUI TGTACA 1 cut(s) 85
BstBI TTCGAA 2 cut(s) 648, 1122
BstC8I GCNNGC 5 cut(s) 274, 296, 446, 489, 905
BstDEI CTNAG 7 cut(s) 339, 360, 804, 1064, 1262, 1385, 1571
BstDSI CCRYGG 1 cut(s) 959
BstF5I GGATG 8 cut(s) 328, 364, 765, 955, 1237, 1246, 1408, 1477
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 7 cut(s) 345, 913, 955, 1023, 1096, 1191, 1210
BstMAI GTCTC 3 cut(s) 48, 248, 851
BstMBI GATC 7 cut(s) 342, 910, 952, 1020, 1093, 1188, 1207
BstMWI GCNNNNNNNGC 6 cut(s) 15, 355, 441, 534, 1241, 1263
BstNI CCWGG 3 cut(s) 508, 530, 654
BstNSI RCATGY 1 cut(s) 907
BstSCI CCNGG 3 cut(s) 506, 528, 652
BstSFI CTRYAG 1 cut(s) 235
BstV1I GCAGC 3 cut(s) 478, 1222, 1253
BstV2I GAAGAC 1 cut(s) 308
BstXI CCANNNNNNTGG 1 cut(s) 545
BsuRI GGCC 2 cut(s) 633, 727
BtgI CCRYGG 1 cut(s) 959
BtgZI GCGATG 2 cut(s) 1284, 1329
BtsCI GGATG 8 cut(s) 328, 364, 765, 955, 1237, 1246, 1408, 1477
BtsI GCAGTG 1 cut(s) 88
BtsIMutI CAGTG 3 cut(s) 88, 498, 1259
BveI ACCTGC 4 cut(s) 23, 31, 96, 1420
Cac8I GCNNGC 5 cut(s) 274, 296, 446, 489, 905
CaiI CAGNNNCTG 1 cut(s) 803
CfoI GCGC 1 cut(s) 351
Cfr10I RCCGGY 2 cut(s) 121, 167
Cfr13I GGNCC 3 cut(s) 562, 725, 1415
CseI GACGC 1 cut(s) 232
CsiI ACCWGGT 1 cut(s) 652
Csp6I GTAC 4 cut(s) 86, 190, 837, 1568
CspAI ACCGGT 1 cut(s) 121
CviAII CATG 7 cut(s) 346, 424, 545, 694, 861, 904, 1519
CviQI GTAC 4 cut(s) 86, 190, 837, 1568
DdeI CTNAG 7 cut(s) 339, 360, 804, 1064, 1262, 1385, 1571
DpnI GATC 7 cut(s) 344, 912, 954, 1022, 1095, 1190, 1209
DpnII GATC 7 cut(s) 342, 910, 952, 1020, 1093, 1188, 1207
DraI TTTAAA 1 cut(s) 1498
DriI GACNNNNNGTC 1 cut(s) 1199
Eam1104I CTCTTC 3 cut(s) 46, 284, 1485
Eam1105I GACNNNNNGTC 1 cut(s) 1199
EarI CTCTTC 3 cut(s) 46, 284, 1485
EciI GGCGGA 1 cut(s) 1091
Ecl136II GAGCTC 1 cut(s) 448
Eco24I GRGCYC 1 cut(s) 450
Eco47I GGWCC 2 cut(s) 562, 1415
Eco53kI GAGCTC 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 320
EcoICRI GAGCTC 1 cut(s) 448
EcoO109I RGGNCCY 1 cut(s) 562
EcoRI GAATTC 1 cut(s) 1274
EcoRII CCWGG 3 cut(s) 506, 528, 652
EcoT38I GRGCYC 1 cut(s) 450
Esp3I CGTCTC 1 cut(s) 248
FaeI CATG 7 cut(s) 349, 427, 548, 697, 864, 907, 1522
FaqI GGGAC 1 cut(s) 575
FatI CATG 7 cut(s) 345, 423, 544, 693, 860, 903, 1518
Fnu4HI GCNGC 6 cut(s) 10, 442, 492, 495, 1236, 1267
FokI GGATG 8 cut(s) 335, 351, 752, 942, 1244, 1253, 1415, 1484
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 6 cut(s) 10, 442, 492, 495, 1236, 1267
FspBI CTAG 2 cut(s) 893, 950
GlaI GCGC 1 cut(s) 350
GluI GCNGC 6 cut(s) 10, 442, 492, 495, 1236, 1267
HaeIII GGCC 2 cut(s) 633, 727
HapII CCGG 4 cut(s) 122, 168, 701, 728
HgaI GACGC 1 cut(s) 232
HhaI GCGC 1 cut(s) 351
Hin1II CATG 7 cut(s) 349, 427, 548, 697, 864, 907, 1522
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HincII GTYRAC 1 cut(s) 1459
HindII GTYRAC 1 cut(s) 1459
HindIII AAGCTT 1 cut(s) 1332
HinfI GANTC 6 cut(s) 92, 228, 663, 976, 1107, 1595
HpaII CCGG 4 cut(s) 122, 168, 701, 728
HphI GGTGA 1 cut(s) 223
Hpy166II GTNNAC 5 cut(s) 86, 88, 247, 1294, 1459
Hpy188I TCNGA 5 cut(s) 369, 660, 877, 1112, 1193
Hpy188III TCNNGA 7 cut(s) 701, 850, 1051, 1172, 1211, 1418, 1551
Hpy8I GTNNAC 5 cut(s) 86, 88, 247, 1294, 1459
Hpy99I CGWCG 1 cut(s) 100
HpyAV CCTTC 2 cut(s) 431, 803
HpyCH4III ACNGT 1 cut(s) 502
HpyCH4V TGCA 8 cut(s) 18, 119, 276, 590, 608, 818, 924, 1219
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 355, 441, 534, 1241, 1263
HpyF3I CTNAG 7 cut(s) 339, 360, 804, 1064, 1262, 1385, 1571
Hsp92II CATG 7 cut(s) 349, 427, 548, 697, 864, 907, 1522
HspAI GCGC 1 cut(s) 349
Kpn2I TCCGGA 1 cut(s) 700
Kzo9I GATC 7 cut(s) 342, 910, 952, 1020, 1093, 1188, 1207
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 4 cut(s) 427, 484, 703, 900
Lsp1109I GCAGC 3 cut(s) 478, 1222, 1253
LweI GCATC 4 cut(s) 230, 373, 1222, 1231
MabI ACCWGGT 1 cut(s) 652
MaeI CTAG 2 cut(s) 893, 950
MaeIII GTNAC 3 cut(s) 502, 1009, 1599
MalI GATC 7 cut(s) 344, 912, 954, 1022, 1095, 1190, 1209
MboI GATC 7 cut(s) 342, 910, 952, 1020, 1093, 1188, 1207
MboII GAAGA 9 cut(s) 63, 172, 301, 313, 953, 1066, 1175, 1198, 1502
MfeI CAATTG 1 cut(s) 621
MhlI GDGCHC 1 cut(s) 450
MluCI AATT 9 cut(s) 206, 577, 611, 621, 672, 718, 813, 1274, 1319
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 2 cut(s) 658, 1209
MroI TCCGGA 1 cut(s) 700
MroXI GAANNNNTTC 2 cut(s) 1046, 1167
MseI TTAA 5 cut(s) 614, 1016, 1037, 1298, 1497
MslI CAYNNNNRTG 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 1362
MspI CCGG 4 cut(s) 122, 168, 701, 728
MspR9I CCNGG 3 cut(s) 508, 530, 654
MunI CAATTG 1 cut(s) 621
MvaI CCWGG 3 cut(s) 508, 530, 654
MwoI GCNNNNNNNGC 6 cut(s) 15, 355, 441, 534, 1241, 1263
NdeII GATC 7 cut(s) 342, 910, 952, 1020, 1093, 1188, 1207
NlaIII CATG 7 cut(s) 349, 427, 548, 697, 864, 907, 1522
NlaIV GGNNCC 3 cut(s) 126, 563, 699
NmuCI GTSAC 1 cut(s) 1009
NspI RCATGY 1 cut(s) 907
NspV TTCGAA 2 cut(s) 648, 1122
PaeI GCATGC 1 cut(s) 907
PaqCI CACCTGC 1 cut(s) 31
PciSI GCTCTTC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 1604
PdmI GAANNNNTTC 2 cut(s) 1046, 1167
PfeI GAWTC 5 cut(s) 228, 663, 976, 1107, 1595
PinAI ACCGGT 1 cut(s) 121
PkrI GCNGC 6 cut(s) 11, 443, 493, 496, 1237, 1268
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
PpuMI RGGWCCY 1 cut(s) 562
Psp124BI GAGCTC 1 cut(s) 450
Psp5II RGGWCCY 1 cut(s) 562
Psp6I CCWGG 3 cut(s) 506, 528, 652
PspGI CCWGG 3 cut(s) 506, 528, 652
PspN4I GGNNCC 3 cut(s) 126, 563, 699
PspPI GGNCC 3 cut(s) 562, 725, 1415
PspPPI RGGWCCY 1 cut(s) 562
PstNI CAGNNNCTG 1 cut(s) 803
RsaI GTAC 4 cut(s) 87, 191, 838, 1569
RsaNI GTAC 4 cut(s) 86, 190, 837, 1568
RseI CAYNNNNRTG 1 cut(s) 543
SacI GAGCTC 1 cut(s) 450
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 5 cut(s) 614, 1016, 1037, 1298, 1497
SatI GCNGC 6 cut(s) 10, 442, 492, 495, 1236, 1267
Sau3AI GATC 7 cut(s) 342, 910, 952, 1020, 1093, 1188, 1207
Sau96I GGNCC 3 cut(s) 562, 725, 1415
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 3 cut(s) 508, 530, 654
SduI GDGCHC 1 cut(s) 450
SexAI ACCWGGT 1 cut(s) 652
SfaNI GCATC 4 cut(s) 230, 373, 1222, 1231
SfcI CTRYAG 1 cut(s) 235
SfuI TTCGAA 2 cut(s) 648, 1122
SinI GGWCC 2 cut(s) 562, 1415
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
SpeI ACTAGT 1 cut(s) 892
SphI GCATGC 1 cut(s) 907
Sse9I AATT 9 cut(s) 206, 577, 611, 621, 672, 718, 813, 1274, 1319
SsiI CCGC 5 cut(s) 441, 495, 1076, 1238, 1362
SspI AATATT 2 cut(s) 373, 1547
SspMI CTAG 2 cut(s) 893, 950
SstI GAGCTC 1 cut(s) 450
StyD4I CCNGG 3 cut(s) 506, 528, 652
TaaI ACNGT 1 cut(s) 502
TaqI TCGA 9 cut(s) 287, 318, 648, 849, 1122, 1171, 1347, 1377, 1550
TasI AATT 9 cut(s) 206, 577, 611, 621, 672, 718, 813, 1274, 1319
TatI WGTACW 2 cut(s) 85, 1567
TauI GCSGC 2 cut(s) 444, 497
TfiI GAWTC 5 cut(s) 228, 663, 976, 1107, 1595
Tru1I TTAA 5 cut(s) 614, 1016, 1037, 1298, 1497
Tru9I TTAA 5 cut(s) 614, 1016, 1037, 1298, 1497
TscAI CASTG 3 cut(s) 88, 505, 1266
TseFI GTSAC 1 cut(s) 1009
TseI GCWGC 4 cut(s) 9, 491, 1235, 1266
Tsp45I GTSAC 1 cut(s) 1009
TspDTI ATGAA 5 cut(s) 219, 1055, 1287, 1332, 1523
TspGWI ACGGA 4 cut(s) 320, 497, 948, 1161
TspRI CASTG 3 cut(s) 88, 505, 1266
VpaK11BI GGWCC 2 cut(s) 562, 1415
XapI RAATTY 3 cut(s) 577, 1274, 1319
XceI RCATGY 1 cut(s) 907
XcmI CCANNNNNNNNNTGG 1 cut(s) 1334
XmnI GAANNNNTTC 2 cut(s) 1046, 1167
XspI CTAG 2 cut(s) 893, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.