MD17G1082100.v1.1

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0017037
Physical Location & Seq
Forward (+)
6852505 .. 6855141
2637 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1082100.v1.1.491

Sequence Viewer

Length: 1626 bp
ATGGGAAGCTGCTTATGCACACCTGCGAACGCAGGTGTCCACCACAGACGAAGAAACATCATCGAACCTCTACCTCAAAACAGTGTACATGACTCGTCACCGCGCAAGTCGTCCGTTGCAGCGGTTCCCCAGCACCGCAGTATTATATGGGTGCTGGACAAGACTGCTGGTGATGACATCTTCGGCAAGTACCTATTTGGCAAGGAATTAGGGCGAGGGGAGTTTGGAATCACTTACAGATGCGTCCACAGGGAGACGGGGGAGCCTTTTTCCTGCAAGACCATATCGAAGAGCAAGCTGAAGACGGAGATAGATGTCGAGGATGTAAGGAGGGAGGCTGAGATCATGCGCCGTTTGCCTAAGCATCTGAATATTGTCTGCTTCAAGGAGGCTTATGAGGATAGGGAGGCAGTTTATCTTGTCATGGAGCTTTGCGAAGGCGGCGAGCTCTTTGATAGGATTGTGGCCAAAGGGCATTACACGGAGCGCGCTGCAGCCAATGTTACCAGGACAATGCTTGAGGTTTGCCAGGTATGCCACAAGCATGGAGTGATACATAGGGACATGAAACCGGAGAATTTCCTTTTTGCAGATAGTAGTGAAAATGCACAATTGAAGGCAATTGATTTTGGCCTTGGTATATTTTTCGAACCAGGTCAGAGATTCACTGAAATTGTTGGAAGTCCATATTACATGGCTCCGGAGGTGCTACGGCGTAATTATGGATCGGAAGTTGATGTTTGGAGTGCAGGGGTTATCCTATACATCCTACTTTGTGGAGTGCCTCCATTTTGGGCAGAATCTGAGGAAGGAATTGCACAGGCTATTGTTCGGGGTACCATAGATTTCGAGAGGGAGACATGGAAACAGGTTTCGGAGGAAGCAAAAGAACTAGTGAGGCGCATGCTTGATCCAAACCCTCACAACAGGCTCACAGTTGAAGAAGTCCTCGATCATCCATGGATACGAAACGCAAATCAAGTTCCTAATGTTCCTCTGGGAGAAAATGTCACATTAAGGATCAAGCAGTTCTCGTTAATGAATAAGTTCAAGAAGAAAGTTCTTAGAGTGGTGGCGGGTAACTTGCCAGAGGATCAAATAGGTGGAATCAGACAGATATTCGAAATGATGGACATTGACAACAACGGAAACTTGACTTTTGAAGAACTTCGAGATGGTCTTGTGAAGATCGGACACAATGTTGCTGATCCTGATGTGCACATGTTGTTGGAAGCTGCGGATGCTGATGGAAATGGCACACTGAGCTGCGAGGAATTCGTTATGGTGTCTGTTCATTTAAAAAAGATAGACAGCGATGAATTTCTCCATCAAGCTTTTGGCTATTTCGACAAGAATAACAGCGGATATATTGAGTTCGAGGAGTTGAGAGAAGCCTTGCTCGATGACACACTTGGTCCGGCTAATGAGCAGGTTATACAAGACATCATATTCGATGTTGACTTGGACAAGGATGGGCGAATAAGTTTTGAAGAGTTTAAAGGAATGATGAAAACTGGCATGGACTGGAAAATGTCTTCTCGTCAATATTCGAGGGCATTGCTAAATGTACTTAGCCGTAAGTTGTTGAAAGACGAATCGTTACAAGCGAACTATAACGAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010769 GO:0010857 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043269 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0046777 GO:0047484 GO:0048519 GO:0048583 GO:0048585 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080092 GO:0080134 GO:0097305 GO:0097306 GO:0140096 GO:1901000 GO:1901001 GO:1901016 GO:1901379 GO:1901564 GO:1901700 GO:1901701 GO:1901979 GO:1904062 GO:2000241 GO:2001257
KEGG Pathways
Metabolic & Signaling
ko04626 ko05145 map04626 map05145
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

542

Amino Acids

61.62

Weight (kDa)

5.33

Isoelectric Point (pI)

45.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 66 - 322 1.1e-77 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 66 - 317 5.1e-27 Protein tyrosine and serine/threonine kinase
EF-hand_1 PF00036 370 - 396 5.3e-06 EF hand domain
EF-hand_7 PF13499 370 - 427 1.6e-08 EF-hand domain pair
EF-hand_8 PF13833 383 - 432 8.4e-06 EF-hand domain pair
EF-hand_7 PF13499 440 - 504 1.5e-13 EF-hand domain pair
EF-hand_1 PF00036 442 - 466 3.3e-06 EF hand domain
EF-hand_6 PF13405 442 - 467 5.7e-06 EF-hand domain
EF-hand_1 PF00036 479 - 505 2.4e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31500
fragaria_vesca FvH4_4g11140
malus_domestica MD09G1092200.v1.1 MD17G1082100.v1.1
prunus_persica Prupe.1G190700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0407071
rosa_laevigata RLG00000008739
rosa_multiflora Rmu_sc0000600.1_g000002
rosa_rugosa Rorug04G0077800
rosa_samantha Rh4AG142500 Rh4CG149000 Rh4DG136600
rosa_wichuraiana Rw4G011610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 23, 31
Acc36I ACCTGC 3 cut(s) 23, 31, 1420
Acc65I GGTACC 1 cut(s) 836
AccB1I GGYRCC 1 cut(s) 836
AccII CGCG 2 cut(s) 103, 489
AccIII TCCGGA 1 cut(s) 700
AciI CCGC 7 cut(s) 101, 122, 136, 441, 1076, 1238, 1362
AclWI GGATC 5 cut(s) 733, 905, 1028, 1101, 1202
AcoI YGGCCR 1 cut(s) 465
AcsI RAATTY 3 cut(s) 577, 1274, 1319
AcuI CTGAAG 1 cut(s) 320
AfaI GTAC 4 cut(s) 87, 191, 838, 1569
AflIII ACRYGT 1 cut(s) 1221
AgsI TTSAA 7 cut(s) 385, 616, 941, 1051, 1163, 1490, 1588
AhlI ACTAGT 1 cut(s) 892
AjnI CCWGG 3 cut(s) 506, 528, 652
AluBI AGCT 7 cut(s) 9, 298, 430, 448, 1235, 1266, 1334
AluI AGCT 7 cut(s) 9, 298, 430, 448, 1235, 1266, 1334
Alw21I GWGCWC 2 cut(s) 450, 1221
Alw26I GTCTC 2 cut(s) 248, 851
Alw44I GTGCAC 1 cut(s) 1217
AlwI GGATC 5 cut(s) 733, 905, 1028, 1101, 1202
AlwNI CAGNNNCTG 1 cut(s) 803
Aor13HI TCCGGA 1 cut(s) 700
AoxI GGCC 2 cut(s) 465, 631
ApaLI GTGCAC 1 cut(s) 1217
ApeKI GCWGC 6 cut(s) 9, 119, 491, 494, 1235, 1266
ApoI RAATTY 3 cut(s) 577, 1274, 1319
ArsI GACNNNNNNTTYG 2 cut(s) 1433, 1465
Asp700I GAANNNNTTC 2 cut(s) 1046, 1167
Asp718I GGTACC 1 cut(s) 836
AspLEI GCGC 5 cut(s) 105, 351, 489, 491, 903
AspS9I GGNCC 1 cut(s) 1415
AsuHPI GGTGA 2 cut(s) 90, 182
AsuII TTCGAA 2 cut(s) 648, 1122
AvaII GGWCC 1 cut(s) 1415
BaeGI GKGCMC 1 cut(s) 1221
BalI TGGCCA 1 cut(s) 467
BanI GGYRCC 1 cut(s) 836
BanII GRGCYC 1 cut(s) 450
BbsI GAAGAC 2 cut(s) 308, 1527
Bbv12I GWGCWC 2 cut(s) 450, 1221
BbvI GCAGC 5 cut(s) 131, 478, 506, 1222, 1253
BccI CCATC 5 cut(s) 1123, 1169, 1241, 1335, 1466
BceAI ACGGC 3 cut(s) 336, 728, 1560
BcgI CGANNNNNNTGC 2 cut(s) 1540, 1574
BciT130I CCWGG 3 cut(s) 508, 530, 654
BciVI GTATCC 1 cut(s) 957
BcoDI GTCTC 2 cut(s) 248, 851
BcuI ACTAGT 1 cut(s) 892
BfaI CTAG 1 cut(s) 893
BfmI CTRYAG 1 cut(s) 492
BfuAI ACCTGC 3 cut(s) 23, 31, 1420
BfuI GTATCC 1 cut(s) 957
BisI GCNGC 7 cut(s) 10, 120, 442, 492, 495, 1236, 1267
BlsI GCNGC 7 cut(s) 11, 121, 443, 493, 496, 1237, 1268
Bme1390I CCNGG 3 cut(s) 508, 530, 654
Bme18I GGWCC 1 cut(s) 1415
BmgT120I GGNCC 1 cut(s) 1415
BmiI GGNNCC 4 cut(s) 126, 264, 699, 838
BmrFI CCNGG 3 cut(s) 508, 530, 654
BmsI GCATC 3 cut(s) 230, 373, 1231
BpiI GAAGAC 2 cut(s) 308, 1527
Bpu10I CCTNAGC 1 cut(s) 360
Bpu14I TTCGAA 2 cut(s) 648, 1122
BpuEI CTTGAG 1 cut(s) 539
BsaJI CCNNGG 2 cut(s) 634, 959
BsaWI WCCGGW 2 cut(s) 571, 700
BsaXI ACNNNNNCTCC 2 cut(s) 993, 1023
Bse1I ACTGG 2 cut(s) 1519, 1529
Bse3DI GCAATG 1 cut(s) 1556
BseAI TCCGGA 1 cut(s) 700
BseBI CCWGG 3 cut(s) 508, 530, 654
BseDI CCNNGG 2 cut(s) 634, 959
BseGI GGATG 5 cut(s) 328, 765, 955, 1246, 1477
BseMI GCAATG 1 cut(s) 1556
BseMII CTCAG 3 cut(s) 330, 795, 1253
BseNI ACTGG 2 cut(s) 1519, 1529
BsePI GCGCGC 1 cut(s) 487
BseRI GAGGAG 1 cut(s) 1394
BseSI GKGCMC 1 cut(s) 1221
BseXI GCAGC 5 cut(s) 131, 478, 506, 1222, 1253
BseYI CCCAGC 1 cut(s) 129
BsgI GTGCAG 1 cut(s) 768
Bsh1236I CGCG 2 cut(s) 103, 489
BshFI GGCC 2 cut(s) 467, 633
BshNI GGYRCC 1 cut(s) 836
BsiHKAI GWGCWC 2 cut(s) 450, 1221
BsiSI CCGG 3 cut(s) 572, 701, 1418
BslFI GGGAC 1 cut(s) 575
BsmAI GTCTC 2 cut(s) 248, 851
BsmBI CGTCTC 1 cut(s) 248
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 2 cut(s) 467, 633
Bsp119I TTCGAA 2 cut(s) 648, 1122
Bsp1286I GDGCHC 2 cut(s) 450, 1221
Bsp13I TCCGGA 1 cut(s) 700
Bsp1407I TGTACA 1 cut(s) 85
Bsp143I GATC 8 cut(s) 342, 725, 910, 952, 1020, 1093, 1188, 1207
Bsp19I CCATGG 1 cut(s) 959
BspACI CCGC 7 cut(s) 101, 122, 136, 441, 1076, 1238, 1362
BspANI GGCC 2 cut(s) 467, 633
BspCNI CTCAG 3 cut(s) 331, 796, 1254
BspEI TCCGGA 1 cut(s) 700
BspFNI CGCG 2 cut(s) 103, 489
BspLI GGNNCC 4 cut(s) 126, 264, 699, 838
BspMAI CTGCAG 1 cut(s) 496
BspMI ACCTGC 3 cut(s) 23, 31, 1420
BspPI GGATC 5 cut(s) 733, 905, 1028, 1101, 1202
BspQI GCTCTTC 1 cut(s) 284
BspT104I TTCGAA 2 cut(s) 648, 1122
BspT107I GGYRCC 1 cut(s) 836
BsrDI GCAATG 1 cut(s) 1556
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 2 cut(s) 1519, 1529
BssECI CCNNGG 2 cut(s) 634, 959
BssHII GCGCGC 1 cut(s) 487
BssMI GATC 8 cut(s) 342, 725, 910, 952, 1020, 1093, 1188, 1207
BssT1I CCWWGG 2 cut(s) 634, 959
Bst2UI CCWGG 3 cut(s) 508, 530, 654
Bst4CI ACNGT 2 cut(s) 83, 937
Bst6I CTCTTC 2 cut(s) 284, 1485
BstAUI TGTACA 1 cut(s) 85
BstBI TTCGAA 2 cut(s) 648, 1122
BstC8I GCNNGC 4 cut(s) 296, 446, 489, 905
BstDEI CTNAG 6 cut(s) 339, 360, 804, 1064, 1262, 1571
BstDSI CCRYGG 1 cut(s) 959
BstF5I GGATG 5 cut(s) 328, 765, 955, 1246, 1477
BstFNI CGCG 2 cut(s) 103, 489
BstHHI GCGC 5 cut(s) 105, 351, 489, 491, 903
BstKTI GATC 8 cut(s) 345, 728, 913, 955, 1023, 1096, 1191, 1210
BstMAI GTCTC 2 cut(s) 248, 851
BstMBI GATC 8 cut(s) 342, 725, 910, 952, 1020, 1093, 1188, 1207
BstMWI GCNNNNNNNGC 6 cut(s) 15, 355, 441, 534, 1241, 1263
BstNI CCWGG 3 cut(s) 508, 530, 654
BstNSI RCATGY 2 cut(s) 907, 1225
BstSCI CCNGG 3 cut(s) 506, 528, 652
BstSFI CTRYAG 1 cut(s) 492
BstSLI GKGCMC 1 cut(s) 1221
BstUI CGCG 2 cut(s) 103, 489
BstV1I GCAGC 5 cut(s) 131, 478, 506, 1222, 1253
BstV2I GAAGAC 2 cut(s) 308, 1527
BstXI CCANNNNNNTGG 1 cut(s) 545
BsuI GTATCC 1 cut(s) 957
BsuRI GGCC 2 cut(s) 467, 633
BtgI CCRYGG 1 cut(s) 959
BtgZI GCGATG 1 cut(s) 1329
BtsCI GGATG 5 cut(s) 328, 765, 955, 1246, 1477
BtsIMutI CAGTG 3 cut(s) 88, 666, 1259
BveI ACCTGC 3 cut(s) 23, 31, 1420
Cac8I GCNNGC 4 cut(s) 296, 446, 489, 905
CaiI CAGNNNCTG 1 cut(s) 803
CfoI GCGC 5 cut(s) 105, 351, 489, 491, 903
Cfr13I GGNCC 1 cut(s) 1415
CseI GACGC 1 cut(s) 232
CsiI ACCWGGT 1 cut(s) 652
Csp6I GTAC 4 cut(s) 86, 190, 837, 1568
CviQI GTAC 4 cut(s) 86, 190, 837, 1568
DdeI CTNAG 6 cut(s) 339, 360, 804, 1064, 1262, 1571
DpnI GATC 8 cut(s) 344, 727, 912, 954, 1022, 1095, 1190, 1209
DpnII GATC 8 cut(s) 342, 725, 910, 952, 1020, 1093, 1188, 1207
DraI TTTAAA 2 cut(s) 1299, 1498
EaeI YGGCCR 1 cut(s) 465
Eam1104I CTCTTC 2 cut(s) 284, 1485
EarI CTCTTC 2 cut(s) 284, 1485
Ecl136II GAGCTC 1 cut(s) 448
Eco130I CCWWGG 2 cut(s) 634, 959
Eco24I GRGCYC 1 cut(s) 450
Eco47I GGWCC 1 cut(s) 1415
Eco53kI GAGCTC 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 320
EcoICRI GAGCTC 1 cut(s) 448
EcoRI GAATTC 1 cut(s) 1274
EcoRII CCWGG 3 cut(s) 506, 528, 652
EcoT14I CCWWGG 2 cut(s) 634, 959
EcoT38I GRGCYC 1 cut(s) 450
ErhI CCWWGG 2 cut(s) 634, 959
Esp3I CGTCTC 1 cut(s) 248
FaqI GGGAC 1 cut(s) 575
FauI CCCGC 1 cut(s) 1069
Fnu4HI GCNGC 7 cut(s) 10, 120, 442, 492, 495, 1236, 1267
FokI GGATG 5 cut(s) 335, 752, 942, 1253, 1484
FriOI GRGCYC 1 cut(s) 450
Fsp4HI GCNGC 7 cut(s) 10, 120, 442, 492, 495, 1236, 1267
FspBI CTAG 1 cut(s) 893
GlaI GCGC 5 cut(s) 104, 350, 488, 490, 902
GluI GCNGC 7 cut(s) 10, 120, 442, 492, 495, 1236, 1267
GsaI CCCAGC 1 cut(s) 133
HaeIII GGCC 2 cut(s) 467, 633
HapII CCGG 3 cut(s) 572, 701, 1418
HgaI GACGC 1 cut(s) 232
HhaI GCGC 5 cut(s) 105, 351, 489, 491, 903
Hin6I GCGC 5 cut(s) 103, 349, 487, 489, 901
HinP1I GCGC 5 cut(s) 103, 349, 487, 489, 901
HincII GTYRAC 1 cut(s) 1459
HindII GTYRAC 1 cut(s) 1459
HindIII AAGCTT 1 cut(s) 1332
HinfI GANTC 6 cut(s) 92, 228, 663, 800, 1107, 1595
HpaII CCGG 3 cut(s) 572, 701, 1418
HphI GGTGA 2 cut(s) 90, 182
Hpy166II GTNNAC 5 cut(s) 40, 86, 247, 1219, 1459
Hpy188I TCNGA 7 cut(s) 369, 660, 730, 805, 877, 1112, 1193
Hpy188III TCNNGA 5 cut(s) 701, 850, 1051, 1172, 1211
Hpy8I GTNNAC 5 cut(s) 40, 86, 247, 1219, 1459
HpyAV CCTTC 3 cut(s) 431, 610, 803
HpyCH4III ACNGT 2 cut(s) 83, 937
HpyCH4V TGCA 9 cut(s) 18, 119, 276, 494, 590, 608, 749, 818, 1219
HpyF10VI GCNNNNNNNGC 6 cut(s) 15, 355, 441, 534, 1241, 1263
HpyF3I CTNAG 6 cut(s) 339, 360, 804, 1064, 1262, 1571
HspAI GCGC 5 cut(s) 103, 349, 487, 489, 901
Kpn2I TCCGGA 1 cut(s) 700
KpnI GGTACC 1 cut(s) 840
Kzo9I GATC 8 cut(s) 342, 725, 910, 952, 1020, 1093, 1188, 1207
LguI GCTCTTC 1 cut(s) 284
LmnI GCTCC 4 cut(s) 262, 427, 484, 703
Lsp1109I GCAGC 5 cut(s) 131, 478, 506, 1222, 1253
LweI GCATC 3 cut(s) 230, 373, 1231
MabI ACCWGGT 1 cut(s) 652
MaeI CTAG 1 cut(s) 893
MaeIII GTNAC 5 cut(s) 96, 502, 1009, 1079, 1599
MalI GATC 8 cut(s) 344, 727, 912, 954, 1022, 1095, 1190, 1209
MboI GATC 8 cut(s) 342, 725, 910, 952, 1020, 1093, 1188, 1207
MfeI CAATTG 2 cut(s) 611, 621
MhlI GDGCHC 2 cut(s) 450, 1221
MlsI TGGCCA 1 cut(s) 467
MluCI AATT 9 cut(s) 206, 577, 611, 621, 672, 718, 813, 1274, 1319
MluNI TGGCCA 1 cut(s) 467
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 2 cut(s) 658, 1209
Mox20I TGGCCA 1 cut(s) 467
MroI TCCGGA 1 cut(s) 700
MroXI GAANNNNTTC 2 cut(s) 1046, 1167
MscI TGGCCA 1 cut(s) 467
MseI TTAA 4 cut(s) 1016, 1037, 1298, 1497
MslI CAYNNNNRTG 1 cut(s) 543
Msp20I TGGCCA 1 cut(s) 467
MspA1I CMGCKG 2 cut(s) 122, 1362
MspI CCGG 3 cut(s) 572, 701, 1418
MspR9I CCNGG 3 cut(s) 508, 530, 654
MunI CAATTG 2 cut(s) 611, 621
MvaI CCWGG 3 cut(s) 508, 530, 654
MvnI CGCG 2 cut(s) 103, 489
MwoI GCNNNNNNNGC 6 cut(s) 15, 355, 441, 534, 1241, 1263
NcoI CCATGG 1 cut(s) 959
NdeII GATC 8 cut(s) 342, 725, 910, 952, 1020, 1093, 1188, 1207
NlaIV GGNNCC 4 cut(s) 126, 264, 699, 838
NmuCI GTSAC 2 cut(s) 96, 1009
NspI RCATGY 2 cut(s) 907, 1225
NspV TTCGAA 2 cut(s) 648, 1122
PaeI GCATGC 1 cut(s) 907
PaqCI CACCTGC 2 cut(s) 23, 31
PauI GCGCGC 1 cut(s) 487
PciI ACATGT 1 cut(s) 1221
PciSI GCTCTTC 1 cut(s) 284
PcsI WCGNNNNNNNCGW 1 cut(s) 1604
PdmI GAANNNNTTC 2 cut(s) 1046, 1167
PfeI GAWTC 5 cut(s) 228, 663, 800, 1107, 1595
PkrI GCNGC 7 cut(s) 11, 121, 443, 493, 496, 1237, 1268
PleI GAGTC 1 cut(s) 86
PpsI GAGTC 1 cut(s) 86
PscI ACATGT 1 cut(s) 1221
Psp124BI GAGCTC 1 cut(s) 450
Psp6I CCWGG 3 cut(s) 506, 528, 652
PspFI CCCAGC 1 cut(s) 129
PspGI CCWGG 3 cut(s) 506, 528, 652
PspN4I GGNNCC 4 cut(s) 126, 264, 699, 838
PspPI GGNCC 1 cut(s) 1415
PstI CTGCAG 1 cut(s) 496
PstNI CAGNNNCTG 1 cut(s) 803
PteI GCGCGC 1 cut(s) 487
RsaI GTAC 4 cut(s) 87, 191, 838, 1569
RsaNI GTAC 4 cut(s) 86, 190, 837, 1568
RseI CAYNNNNRTG 1 cut(s) 543
SacI GAGCTC 1 cut(s) 450
SapI GCTCTTC 1 cut(s) 284
SaqAI TTAA 4 cut(s) 1016, 1037, 1298, 1497
SatI GCNGC 7 cut(s) 10, 120, 442, 492, 495, 1236, 1267
Sau3AI GATC 8 cut(s) 342, 725, 910, 952, 1020, 1093, 1188, 1207
Sau96I GGNCC 1 cut(s) 1415
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 3 cut(s) 508, 530, 654
SduI GDGCHC 2 cut(s) 450, 1221
SexAI ACCWGGT 1 cut(s) 652
SfaNI GCATC 3 cut(s) 230, 373, 1231
SfcI CTRYAG 1 cut(s) 492
SfuI TTCGAA 2 cut(s) 648, 1122
SinI GGWCC 1 cut(s) 1415
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 1 cut(s) 518
SmoI CTYRAG 1 cut(s) 518
SpeI ACTAGT 1 cut(s) 892
SphI GCATGC 1 cut(s) 907
Sse9I AATT 9 cut(s) 206, 577, 611, 621, 672, 718, 813, 1274, 1319
SsiI CCGC 7 cut(s) 101, 122, 136, 441, 1076, 1238, 1362
SspI AATATT 2 cut(s) 373, 1547
SspMI CTAG 1 cut(s) 893
SstI GAGCTC 1 cut(s) 450
StyD4I CCNGG 3 cut(s) 506, 528, 652
StyI CCWWGG 2 cut(s) 634, 959
TaaI ACNGT 2 cut(s) 83, 937
TasI AATT 9 cut(s) 206, 577, 611, 621, 672, 718, 813, 1274, 1319
TatI WGTACW 2 cut(s) 85, 1567
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 5 cut(s) 228, 663, 800, 1107, 1595
Tru1I TTAA 4 cut(s) 1016, 1037, 1298, 1497
Tru9I TTAA 4 cut(s) 1016, 1037, 1298, 1497
TscAI CASTG 3 cut(s) 88, 673, 1266
TseFI GTSAC 2 cut(s) 96, 1009
TseI GCWGC 6 cut(s) 9, 119, 491, 494, 1235, 1266
Tsp45I GTSAC 2 cut(s) 96, 1009
TspDTI ATGAA 5 cut(s) 581, 1055, 1283, 1332, 1523
TspGWI ACGGA 4 cut(s) 103, 320, 497, 1161
TspRI CASTG 3 cut(s) 88, 673, 1266
VneI GTGCAC 1 cut(s) 1217
VpaK11BI GGWCC 1 cut(s) 1415
XapI RAATTY 3 cut(s) 577, 1274, 1319
XceI RCATGY 2 cut(s) 907, 1225
XcmI CCANNNNNNNNNTGG 1 cut(s) 1334
XmnI GAANNNNTTC 2 cut(s) 1046, 1167
XspI CTAG 1 cut(s) 893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.