FvH4_4g11140

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0017037
Physical Location & Seq
Reverse (-)
14937538 .. 14944472
6935 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g11140.t1

Sequence Viewer

Length: 1587 bp
ATGGGAAGTTGCGTTTGCACACCGGCCAAAGCAGGCTTGCTACACAGAAAACACAGAGATCAGCTGGTCAAGCCCATACCTGCTTACGTCGACTCGCCGAGCCACCACCGTGGGAGCCACAAGGTGCTTGACAAGACCGACGGAGACAACATCTTCAACAAGTACAGGTTAGGCAAGGAGTTGGGGCGTGGGGAGTTTGGTATCACGCACCAATGCGTGGACTCCAAGACCGGCGAGACCTTTGCTTGCAAGATGATATCCAAGAGTAAGTTGAAGACGGAGATTGACATCGAAGACGTGAGGAGGGAGGCCGAGATCATGAAGCGTTTGCCTAACCACCCCAATATTGTCACCTTCAAGGAGGCTTATGAGGACAAGGATGCAGTTTATCTTGTCATGGAGCTCTGCGAGGGTGGTGAGCTTTTCGATAGGATCGTCACCAAAGGCCATTACACAGAGCGCGCCGCCGCTCATGTTACCAAGACAATTCTTGAGGTCTGCAAGGTTTGCCACGAGAATGGAGTGATTCATCGAGACCTGAAACCCGAGAATTTCCTTTTTGCAGATAGTAGTGAAAATGCACAGTTAAAGGCAATTGATTTTGGCCTTTCTGTAATTTTTGAACCAGGTCAGAGATTTTCTGAAATTGTTGGAAGTCCATATTACATGGCTCCGGAGGTTCTGCGACGGAATTATGGGCATGAAGTTGATGTTTGGAGCGCAGGAGTTATCCTCTATATCCTACTTTGTGGAGTTCCTCCATTTTGGGCAGAAACTGAAGAAGGAATTGCACAGGCTATTATTCGGGGTAAAATTGATTTTCAGAAGGATACATGGCAAAAGGTTTCTGAGGAAGCAAAATTATTAGTGAAGAATATGCTGGACCCGAATCCTTACAACAGACTCACAGTTGAAGAAGTCCTTGATCATCCCTGGATACGAAATGCAAATGAAGTTTCGGATGTTCCGCTGGGAGAAAATGTCGGATTAAGGATCAAGCAGTTCACATTGATGAACAAGTTTAAGAAGAAAGTTCTGAGAGTGGTCGCAGACAACTTGCCAGATGAACAAATAGAAGGAATCAGACAGATATTTAACATGATGGACACGGATAACAATGGAGACTTAACTTTTGAAGAGCTTAGAGATGGTCTTCTCAAGATTGGGCACCAAGTCACTGATCCTGATGTGCATATGTTAATTGATGCTGCGGATGTCGATGGAAGTGGCACACTGAACTGTGACGAGTTCGTTATGGTGTCTGTTCACTTGAAAAAGATAAGCAGTGACGAACTTCTTGCGCAAGCTTTTAGCTATTTTGATAAGAACAACAGCAACTATATTGAGTTTGATGAGTTAAAAGAAGCATTGCTGGATGATCAACTGGGGCCAGCTAATGAGCAGGTGATACAAGACATCATATTTGACGTGGACTTGGACAAGGACGGGCGAATCAGTTATGAGGAGTTTAAAGCAATGATGAAAACTGGTATGGATTGGAAAATGGCTTCTCGTCAATATTCAAGAGCAATGCTAAACGTACTTAGCCGAAAATTGTTAAAAGACAAATCGATCCAAGTGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010769 GO:0010857 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043269 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0046777 GO:0047484 GO:0048519 GO:0048583 GO:0048585 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080092 GO:0080134 GO:0097305 GO:0097306 GO:0140096 GO:1901000 GO:1901001 GO:1901016 GO:1901379 GO:1901564 GO:1901700 GO:1901701 GO:1901979 GO:1904062 GO:2000241 GO:2001257
KEGG Pathways
Metabolic & Signaling
ko04626 ko05145 map04626 map05145
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

529

Amino Acids

60.04

Weight (kDa)

5.39

Isoelectric Point (pI)

32.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 55 - 313 4.5e-80 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 57 - 308 2.4e-29 Protein tyrosine and serine/threonine kinase
EF-hand_7 PF13499 359 - 418 9.6e-09 EF-hand domain pair
EF-hand_1 PF00036 361 - 385 6.1e-06 EF hand domain
EF-hand_6 PF13405 361 - 389 5.4e-06 EF-hand domain
EF-hand_8 PF13833 373 - 423 1.1e-06 EF-hand domain pair
EF-hand_7 PF13499 433 - 495 9e-14 EF-hand domain pair
EF-hand_1 PF00036 470 - 496 8.8e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31500
fragaria_vesca FvH4_4g11140
malus_domestica MD09G1092200.v1.1 MD17G1082100.v1.1
prunus_persica Prupe.1G190700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0407071
rosa_laevigata RLG00000008739
rosa_multiflora Rmu_sc0000600.1_g000002
rosa_rugosa Rorug04G0077800
rosa_samantha Rh4AG142500 Rh4CG149000 Rh4DG136600
rosa_wichuraiana Rw4G011610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1393
Acc16I TGCGCA 1 cut(s) 1302
Acc36I ACCTGC 2 cut(s) 88, 1393
AccB1I GGYRCC 1 cut(s) 1167
AccB7I CCANNNNNTGG 1 cut(s) 217
AccBSI CCGCTC 1 cut(s) 470
AccI GTMKAC 1 cut(s) 90
AccII CGCG 1 cut(s) 462
AccIII TCCGGA 1 cut(s) 673
AciI CCGC 4 cut(s) 465, 468, 968, 1211
AclWI GGATC 4 cut(s) 440, 1001, 1175, 1567
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 1 cut(s) 550
AcuI CTGAAG 1 cut(s) 798
AdeI CACNNNGTG 1 cut(s) 124
AfaI GTAC 2 cut(s) 164, 1542
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 8 cut(s) 157, 274, 358, 623, 914, 1136, 1273, 1524
AjiI CACGTC 2 cut(s) 298, 1429
AjnI CCWGG 2 cut(s) 625, 932
AleI CACNNNNGTG 1 cut(s) 108
AluBI AGCT 7 cut(s) 64, 403, 421, 1141, 1307, 1314, 1394
AluI AGCT 7 cut(s) 64, 403, 421, 1141, 1307, 1314, 1394
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 4 cut(s) 138, 230, 528, 1116
AlwI GGATC 4 cut(s) 440, 1001, 1175, 1567
AlwNI CAGNNNCTG 1 cut(s) 776
Ama87I CYCGRG 1 cut(s) 545
Aor13HI TCCGGA 1 cut(s) 673
AoxI GGCC 5 cut(s) 24, 309, 445, 604, 1388
ApeKI GCWGC 1 cut(s) 1208
ApoI RAATTY 1 cut(s) 550
ArsI GACNNNNNNTTYG 4 cut(s) 1115, 1147, 1406, 1438
AspLEI GCGC 4 cut(s) 462, 464, 722, 1303
AspS9I GGNCC 2 cut(s) 883, 1388
AsuHPI GGTGA 4 cut(s) 343, 428, 430, 1417
AvaI CYCGRG 1 cut(s) 545
AvaII GGWCC 1 cut(s) 883
BaeGI GKGCMC 1 cut(s) 1170
BanI GGYRCC 1 cut(s) 1167
BanII GRGCYC 1 cut(s) 405
BauI CACGAG 1 cut(s) 512
BbsI GAAGAC 3 cut(s) 281, 300, 1145
Bbv12I GWGCWC 1 cut(s) 405
BbvI GCAGC 1 cut(s) 1195
BccI CCATC 3 cut(s) 1096, 1142, 1214
BcgI CGANNNNNNTGC 4 cut(s) 224, 258, 1280, 1314
BciT130I CCWGG 2 cut(s) 627, 934
BciVI GTATCC 2 cut(s) 823, 930
BclI TGATCA 2 cut(s) 925, 1378
BcoDI GTCTC 4 cut(s) 138, 230, 528, 1116
BfuAI ACCTGC 2 cut(s) 88, 1393
BfuI GTATCC 2 cut(s) 823, 930
BisI GCNGC 3 cut(s) 465, 468, 1209
BlsI GCNGC 3 cut(s) 466, 469, 1210
Bme1390I CCNGG 2 cut(s) 627, 934
Bme18I GGWCC 1 cut(s) 883
BmeT110I CYCGRG 1 cut(s) 545
BmgBI CACGTC 2 cut(s) 298, 1429
BmgT120I GGNCC 2 cut(s) 883, 1388
BmiI GGNNCC 5 cut(s) 116, 672, 885, 1169, 1389
BmrFI CCNGG 2 cut(s) 627, 934
BmrI ACTGGG 1 cut(s) 1394
BmsI GCATC 2 cut(s) 370, 1195
BmuI ACTGGG 1 cut(s) 1394
BpiI GAAGAC 3 cut(s) 281, 300, 1145
BplI GAGNNNNNCTC 2 cut(s) 717, 749
BpuEI CTTGAG 2 cut(s) 512, 1142
Bsa29I ATCGAT 1 cut(s) 1571
BsaBI GATNNNNATC 1 cut(s) 287
BsaI GGTCTC 2 cut(s) 230, 528
BsaJI CCNNGG 2 cut(s) 109, 932
BsaWI WCCGGW 1 cut(s) 673
BsaXI ACNNNNNCTCC 2 cut(s) 966, 996
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse118I RCCGGY 2 cut(s) 22, 230
Bse1I ACTGG 2 cut(s) 1389, 1492
Bse3DI GCAATG 3 cut(s) 1367, 1482, 1536
Bse8I GATNNNNATC 1 cut(s) 287
BseAI TCCGGA 1 cut(s) 673
BseBI CCWGG 2 cut(s) 627, 934
BseCI ATCGAT 1 cut(s) 1571
BseDI CCNNGG 2 cut(s) 109, 932
BseGI GGATG 5 cut(s) 385, 928, 967, 1219, 1381
BseJI GATNNNNATC 1 cut(s) 287
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 3 cut(s) 1367, 1482, 1536
BseMII CTCAG 2 cut(s) 840, 1028
BseNI ACTGG 2 cut(s) 1389, 1492
BsePI GCGCGC 1 cut(s) 460
BseRI GAGGAG 2 cut(s) 316, 1478
BseSI GKGCMC 1 cut(s) 1170
BseXI GCAGC 1 cut(s) 1195
BseYI CCCAGC 1 cut(s) 970
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 5 cut(s) 26, 311, 447, 606, 1390
BshNI GGYRCC 1 cut(s) 1167
BshVI ATCGAT 1 cut(s) 1571
BsiHKAI GWGCWC 1 cut(s) 405
BsiHKCI CYCGRG 1 cut(s) 545
BsiSI CCGG 3 cut(s) 23, 231, 674
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 4 cut(s) 138, 230, 528, 1116
BsnI GGCC 5 cut(s) 26, 311, 447, 606, 1390
Bso31I GGTCTC 2 cut(s) 230, 528
BsoBI CYCGRG 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 405, 1170
Bsp13I TCCGGA 1 cut(s) 673
Bsp143I GATC 8 cut(s) 58, 315, 432, 925, 993, 1180, 1378, 1572
BspACI CCGC 4 cut(s) 465, 468, 968, 1211
BspANI GGCC 5 cut(s) 26, 311, 447, 606, 1390
BspCNI CTCAG 2 cut(s) 841, 1029
BspDI ATCGAT 1 cut(s) 1571
BspEI TCCGGA 1 cut(s) 673
BspFNI CGCG 1 cut(s) 462
BspHI TCATGA 1 cut(s) 318
BspLI GGNNCC 5 cut(s) 116, 672, 885, 1169, 1389
BspMI ACCTGC 2 cut(s) 88, 1393
BspPI GGATC 4 cut(s) 440, 1001, 1175, 1567
BspQI GCTCTTC 1 cut(s) 1131
BspT107I GGYRCC 1 cut(s) 1167
BspTNI GGTCTC 2 cut(s) 230, 528
BsrBI CCGCTC 1 cut(s) 470
BsrDI GCAATG 3 cut(s) 1367, 1482, 1536
BsrFI RCCGGY 2 cut(s) 22, 230
BsrI ACTGG 2 cut(s) 1389, 1492
BssAI RCCGGY 2 cut(s) 22, 230
BssECI CCNNGG 2 cut(s) 109, 932
BssHII GCGCGC 1 cut(s) 460
BssMI GATC 8 cut(s) 58, 315, 432, 925, 993, 1180, 1378, 1572
BssSI CACGAG 1 cut(s) 512
Bst2BI CACGAG 1 cut(s) 512
Bst2UI CCWGG 2 cut(s) 627, 934
Bst4CI ACNGT 4 cut(s) 110, 585, 910, 1241
Bst6I CTCTTC 1 cut(s) 1131
BstAPI GCANNNNNTGC 1 cut(s) 507
BstC8I GCNNGC 6 cut(s) 34, 38, 247, 462, 1305, 1392
BstDEI CTNAG 4 cut(s) 849, 1037, 1142, 1544
BstDSI CCRYGG 1 cut(s) 109
BstF5I GGATG 5 cut(s) 385, 928, 967, 1219, 1381
BstFNI CGCG 1 cut(s) 462
BstHHI GCGC 4 cut(s) 462, 464, 722, 1303
BstKTI GATC 8 cut(s) 61, 318, 435, 928, 996, 1183, 1381, 1575
BstMAI GTCTC 4 cut(s) 138, 230, 528, 1116
BstMBI GATC 8 cut(s) 58, 315, 432, 925, 993, 1180, 1378, 1572
BstMWI GCNNNNNNNGC 2 cut(s) 70, 507
BstNI CCWGG 2 cut(s) 627, 934
BstSCI CCNGG 2 cut(s) 625, 932
BstSLI GKGCMC 1 cut(s) 1170
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 1 cut(s) 1195
BstV2I GAAGAC 3 cut(s) 281, 300, 1145
BstXI CCANNNNNNTGG 2 cut(s) 110, 518
Bsu15I ATCGAT 1 cut(s) 1571
BsuI GTATCC 2 cut(s) 823, 930
BsuRI GGCC 5 cut(s) 26, 311, 447, 606, 1390
BsuTUI ATCGAT 1 cut(s) 1571
BtgI CCRYGG 1 cut(s) 109
BtrI CACGTC 2 cut(s) 298, 1429
BtsCI GGATG 5 cut(s) 385, 928, 967, 1219, 1381
BtsI GCAGTG 1 cut(s) 1291
BtsIMutI CAGTG 3 cut(s) 1176, 1232, 1291
BveI ACCTGC 2 cut(s) 88, 1393
Cac8I GCNNGC 6 cut(s) 34, 38, 247, 462, 1305, 1392
CaiI CAGNNNCTG 1 cut(s) 776
CciI TCATGA 1 cut(s) 318
CfoI GCGC 4 cut(s) 462, 464, 722, 1303
Cfr10I RCCGGY 2 cut(s) 22, 230
Cfr13I GGNCC 2 cut(s) 883, 1388
ClaI ATCGAT 1 cut(s) 1571
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 2 cut(s) 163, 1541
CviAII CATG 7 cut(s) 319, 397, 473, 667, 701, 834, 1099
CviQI GTAC 2 cut(s) 163, 1541
DdeI CTNAG 4 cut(s) 849, 1037, 1142, 1544
DpnI GATC 8 cut(s) 60, 317, 434, 927, 995, 1182, 1380, 1574
DpnII GATC 8 cut(s) 58, 315, 432, 925, 993, 1180, 1378, 1572
DraI TTTAAA 1 cut(s) 1471
DraIII CACNNNGTG 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 1131
EarI CTCTTC 1 cut(s) 1131
Ecl136II GAGCTC 1 cut(s) 403
Eco24I GRGCYC 1 cut(s) 405
Eco31I GGTCTC 2 cut(s) 230, 528
Eco32I GATATC 1 cut(s) 258
Eco47I GGWCC 1 cut(s) 883
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 1 cut(s) 798
Eco88I CYCGRG 1 cut(s) 545
EcoICRI GAGCTC 1 cut(s) 403
EcoRII CCWGG 2 cut(s) 625, 932
EcoRV GATATC 1 cut(s) 258
EcoT38I GRGCYC 1 cut(s) 405
FaeI CATG 7 cut(s) 322, 400, 476, 670, 704, 837, 1102
FalI AAGNNNNNCTT 2 cut(s) 906, 938
FatI CATG 7 cut(s) 318, 396, 472, 666, 700, 833, 1098
FauNDI CATATG 1 cut(s) 1194
FbaI TGATCA 2 cut(s) 925, 1378
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 3 cut(s) 465, 468, 1209
FokI GGATG 5 cut(s) 392, 915, 974, 1226, 1388
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 3 cut(s) 465, 468, 1209
FspI TGCGCA 1 cut(s) 1302
GlaI GCGC 4 cut(s) 461, 463, 721, 1302
GluI GCNGC 3 cut(s) 465, 468, 1209
GsaI CCCAGC 1 cut(s) 974
HaeIII GGCC 5 cut(s) 26, 311, 447, 606, 1390
HapII CCGG 3 cut(s) 23, 231, 674
HhaI GCGC 4 cut(s) 462, 464, 722, 1303
Hin1II CATG 7 cut(s) 322, 400, 476, 670, 704, 837, 1102
Hin6I GCGC 4 cut(s) 460, 462, 720, 1301
HinP1I GCGC 4 cut(s) 460, 462, 720, 1301
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 1305
HinfI GANTC 7 cut(s) 92, 221, 526, 889, 903, 1080, 1452
HpaII CCGG 3 cut(s) 23, 231, 674
HphI GGTGA 4 cut(s) 343, 428, 430, 1417
Hpy166II GTNNAC 5 cut(s) 91, 220, 1005, 1267, 1432
Hpy188I TCNGA 8 cut(s) 633, 643, 825, 850, 961, 986, 1038, 1085
Hpy188III TCNNGA 7 cut(s) 319, 491, 533, 674, 1159, 1184, 1524
Hpy8I GTNNAC 5 cut(s) 91, 220, 1005, 1267, 1432
Hpy99I CGWCG 3 cut(s) 92, 143, 690
HpyAV CCTTC 4 cut(s) 364, 776, 820, 1070
HpyCH4III ACNGT 4 cut(s) 110, 585, 910, 1241
HpyCH4IV ACGT 4 cut(s) 87, 297, 1428, 1539
HpyCH4V TGCA 9 cut(s) 18, 249, 383, 501, 563, 581, 791, 947, 1192
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 507
HpyF3I CTNAG 4 cut(s) 849, 1037, 1142, 1544
HpySE526I ACGT 4 cut(s) 87, 297, 1428, 1539
Hsp92II CATG 7 cut(s) 322, 400, 476, 670, 704, 837, 1102
HspAI GCGC 4 cut(s) 460, 462, 720, 1301
Kpn2I TCCGGA 1 cut(s) 673
Ksp22I TGATCA 2 cut(s) 925, 1378
Kzo9I GATC 8 cut(s) 58, 315, 432, 925, 993, 1180, 1378, 1572
LguI GCTCTTC 1 cut(s) 1131
LmnI GCTCC 4 cut(s) 114, 400, 676, 717
Lsp1109I GCAGC 1 cut(s) 1195
LweI GCATC 2 cut(s) 370, 1195
MabI ACCWGGT 1 cut(s) 625
MaeII ACGT 4 cut(s) 87, 297, 1428, 1539
MaeIII GTNAC 6 cut(s) 349, 436, 475, 1174, 1241, 1286
MalI GATC 8 cut(s) 60, 317, 434, 927, 995, 1182, 1380, 1574
MbiI CCGCTC 1 cut(s) 470
MboI GATC 8 cut(s) 58, 315, 432, 925, 993, 1180, 1378, 1572
MboII GAAGA 9 cut(s) 145, 286, 305, 791, 883, 926, 1039, 1145, 1148
MfeI CAATTG 1 cut(s) 594
MhlI GDGCHC 2 cut(s) 405, 1170
MlyI GAGTC 3 cut(s) 86, 215, 897
MmeI TCCRAC 2 cut(s) 631, 964
MroI TCCGGA 1 cut(s) 673
MslI CAYNNNNRTG 3 cut(s) 108, 516, 1010
MspA1I CMGCKG 2 cut(s) 64, 970
MspI CCGG 3 cut(s) 23, 231, 674
MspR9I CCNGG 2 cut(s) 627, 934
MunI CAATTG 1 cut(s) 594
MvaI CCWGG 2 cut(s) 627, 934
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 2 cut(s) 70, 507
NdeI CATATG 1 cut(s) 1194
NdeII GATC 8 cut(s) 58, 315, 432, 925, 993, 1180, 1378, 1572
NlaIII CATG 7 cut(s) 322, 400, 476, 670, 704, 837, 1102
NlaIV GGNNCC 5 cut(s) 116, 672, 885, 1169, 1389
NmeAIII GCCGAG 2 cut(s) 123, 337
NmuCI GTSAC 5 cut(s) 349, 436, 1174, 1241, 1286
NsbI TGCGCA 1 cut(s) 1302
OliI CACNNNNGTG 1 cut(s) 108
PagI TCATGA 1 cut(s) 318
PaqCI CACCTGC 1 cut(s) 1393
PauI GCGCGC 1 cut(s) 460
PciSI GCTCTTC 1 cut(s) 1131
PcsI WCGNNNNNNNCGW 1 cut(s) 432
PfeI GAWTC 4 cut(s) 526, 889, 1080, 1452
PflMI CCANNNNNTGG 1 cut(s) 217
PkrI GCNGC 3 cut(s) 466, 469, 1210
PleI GAGTC 3 cut(s) 86, 215, 897
PpsI GAGTC 3 cut(s) 86, 215, 897
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 2 cut(s) 625, 932
PspFI CCCAGC 1 cut(s) 970
PspGI CCWGG 2 cut(s) 625, 932
PspN4I GGNNCC 5 cut(s) 116, 672, 885, 1169, 1389
PspPI GGNCC 2 cut(s) 883, 1388
PstNI CAGNNNCTG 1 cut(s) 776
PteI GCGCGC 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 64
RsaI GTAC 2 cut(s) 164, 1542
RsaNI GTAC 2 cut(s) 163, 1541
RseI CAYNNNNRTG 3 cut(s) 108, 516, 1010
SacI GAGCTC 1 cut(s) 405
SalI GTCGAC 1 cut(s) 89
SapI GCTCTTC 1 cut(s) 1131
SatI GCNGC 3 cut(s) 465, 468, 1209
Sau3AI GATC 8 cut(s) 58, 315, 432, 925, 993, 1180, 1378, 1572
Sau96I GGNCC 2 cut(s) 883, 1388
SchI GAGTC 3 cut(s) 86, 215, 897
ScrFI CCNGG 2 cut(s) 627, 934
SduI GDGCHC 2 cut(s) 405, 1170
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 2 cut(s) 370, 1195
SinI GGWCC 1 cut(s) 883
SmiMI CAYNNNNRTG 3 cut(s) 108, 516, 1010
SmlI CTYRAG 2 cut(s) 491, 1157
SmoI CTYRAG 2 cut(s) 491, 1157
SsiI CCGC 4 cut(s) 465, 468, 968, 1211
SspI AATATT 2 cut(s) 346, 1520
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 2 cut(s) 625, 932
TaaI ACNGT 4 cut(s) 110, 585, 910, 1241
TaiI ACGT 4 cut(s) 90, 300, 1431, 1542
TaqI TCGA 6 cut(s) 90, 291, 426, 532, 1218, 1571
TaqII GACCGA 1 cut(s) 152
TatI WGTACW 1 cut(s) 162
TauI GCSGC 2 cut(s) 467, 470
TfiI GAWTC 4 cut(s) 526, 889, 1080, 1452
TscAI CASTG 3 cut(s) 1183, 1239, 1291
TseFI GTSAC 5 cut(s) 349, 436, 1174, 1241, 1286
TseI GCWGC 1 cut(s) 1208
Tsp45I GTSAC 5 cut(s) 349, 436, 1174, 1241, 1286
TspDTI ATGAA 7 cut(s) 335, 518, 717, 966, 1028, 1080, 1496
TspGWI ACGGA 4 cut(s) 156, 293, 703, 1124
TspRI CASTG 3 cut(s) 1183, 1239, 1291
Van91I CCANNNNNTGG 1 cut(s) 217
VpaK11BI GGWCC 1 cut(s) 883
XapI RAATTY 1 cut(s) 550
XmiI GTMKAC 1 cut(s) 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.