Rw4G011610

Calcium-dependent protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0017037
Physical Location & Seq
Reverse (-)
27463510 .. 27466402
2893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011610.1

Sequence Viewer

Length: 1641 bp
ATGGGAAGTTGCGTTTGTACACCTGCCAAAGCAGGTTTACTACACAAAAAACACAGAGATCAGGTGATCAAGCCTATACCCCAATGCGTTCATGACTCGCCTCAAAAGTCGTCGAACCACCGCGGGGGCTTCTCGGCCCCCATCTCGGTGCTGGACAAGACGGATGGAGAAAACATCCTTGACAAGTACAAGTTGGGCAAGGAGTTGGGGCGAGGGGAGTTTGGAATCACCCACCGATGCGTGGACCGGCAGACCGGCGAGACCTTCGCGTGCAAGGTGATATCAAAGAGCAAGTTGAAGACAGAGATTGACATTGAAGACGTGAGGAGGGAGGCCGAGATCATGAGGCTTTTGCCCAAGCACCCCAACATCGTCACCTTCAAGGAGGCTTATGAGGACAAGGATGCAGTTTATCTTGTCATGGAGCTCTGTGAGGGAGGCGAGCTTTTCGATAGGATCGTCACAAAAGGCCACTACACAGAGCGCGCCGCCGCCCACGTTACCAAGACAATTCTCGAGGTTTGCAAGATTGGTGTTAAGCGGTTCCTACTTTTTGCAGGTATGCCACGAGCATGGAGTGATTCATCGAGACCTGAAACCCGAGAATTTCTTTTTGCAGATAGTAGTGAAAATGCACAGTTAAAGGCAATTGATTTTGGTCTTTCAGTAAATTATGAACCAGGTCAGAGATTCTACGAAATTGTTGGAAGTCCATATTACATGGCTCCAGAGGTATTACGACGTAATTATGGGCCTGAAGTTGATGTTTGGAGCGCAGGAGTTATCCTCTATATCCTACTTTGTGGAGTTCCTCCATTTTGGGCAGAAACTGAAGAAGGGATTGCACAGGCTATTATTCGGGGTAACATTGATTTTCAGAAGGATACGTGGAAAAAGGTTTCTGAGGAAGCAAAATTACTAGTGAGGAGTATGCTTGACCCAAATCCTTACAACAGACTCACAGTTGAAGAAGTCCTTGATCATCCCTGGATACGAAATGCAAATCAAGTTCCGAATGTTCCTCTGGCAGAAAATGTCAGATTAAGGATCAAGCAGTTCACATTGATGAATAAATTTAAGAAGAAAGTTCTCAGAGTGGTGGCAGACAACCTGCCAGATGAACAAATAGAAGGAATCAGACAGATATTTGATATGATGGACACCGATAACAATGGAGACTTGACTTTTGAAGAGCTTAGGGATGGTCTTCTCAAGATTGGACACCAAGTCACTGATCCTGATGTGCAAATGTTAATTGATGCCGCGGATGTCGATGGAAGTGGCACACTGAACTGTGAGGAGTTCGTTATTGTGTCTGTTCACTTGAAAAAAATAAGCAGCGACGAACTTCTCGCTCAAGCTTTTAGCTATTTTGACAAGAACAACAGCAACTATATTGAATTTGATGAGTTAAGAGAAGCATTGCTGGATGATAAACTTGGTCCTACTAATGAGCAGGTTATCCAAGACATCATATTCGATGTGGACTTGGACAAGGATGGGCGAATCGGTTATGAGGAGTTTAAAGCAATGATGAAAACTGGTATGGACTGGAAAATGGCTTCTCGTCAATATTCAAGAGCAATGCTAAATGTACTTAGCCGAAAGTTGTTAAAAGACAAGTCGATCCGAGTGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001558 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010769 GO:0010857 GO:0010959 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0022603 GO:0022604 GO:0022898 GO:0023052 GO:0032409 GO:0032412 GO:0032870 GO:0032879 GO:0033993 GO:0034762 GO:0034765 GO:0035556 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043266 GO:0043269 GO:0043412 GO:0043900 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045595 GO:0046777 GO:0047484 GO:0048519 GO:0048583 GO:0048585 GO:0048638 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051128 GO:0051239 GO:0051510 GO:0051716 GO:0060284 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080092 GO:0080134 GO:0097305 GO:0097306 GO:0140096 GO:1901000 GO:1901001 GO:1901016 GO:1901379 GO:1901564 GO:1901700 GO:1901701 GO:1901979 GO:1904062 GO:2000241 GO:2001257
KEGG Pathways
Metabolic & Signaling
ko04626 ko05145 map04626 map05145
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

546

Amino Acids

62.15

Weight (kDa)

5.58

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 63 - 173 3e-26 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 64 - 158 7.6e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 210 - 331 1.3e-33 Protein kinase domain
EF-hand_7 PF13499 377 - 436 2.1e-10 EF-hand domain pair
EF-hand_1 PF00036 379 - 403 9.4e-06 EF hand domain
EF-hand_6 PF13405 379 - 407 7.4e-06 EF-hand domain
EF-hand_8 PF13833 391 - 441 7.4e-08 EF-hand domain pair
EF-hand_7 PF13499 451 - 513 1.4e-13 EF-hand domain pair
EF-hand_1 PF00036 488 - 514 7.2e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017037)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31500
fragaria_vesca FvH4_4g11140
malus_domestica MD09G1092200.v1.1 MD17G1082100.v1.1
prunus_persica Prupe.1G190700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0407071
rosa_laevigata RLG00000008739
rosa_multiflora Rmu_sc0000600.1_g000002
rosa_rugosa Rorug04G0077800
rosa_samantha Rh4AG142500 Rh4CG149000 Rh4DG136600
rosa_wichuraiana Rw4G011610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 5 cut(s) 23, 31, 548, 1119, 1447
AccII CGCG 4 cut(s) 123, 269, 486, 1265
AciI CCGC 7 cut(s) 121, 123, 489, 492, 541, 1263, 1265
AclWI GGATC 4 cut(s) 464, 1055, 1229, 1621
AcsI RAATTY 3 cut(s) 605, 1073, 1400
AcuI CTGAAG 2 cut(s) 777, 852
AfaI GTAC 3 cut(s) 19, 188, 1596
AfiI CCNNNNNNNGG 3 cut(s) 124, 145, 241
AgsI TTSAA 8 cut(s) 298, 317, 382, 968, 1190, 1327, 1400, 1578
AhdI GACNNNNNGTC 1 cut(s) 1226
AhlI ACTAGT 1 cut(s) 919
AjiI CACGTC 1 cut(s) 322
AjnI CCWGG 2 cut(s) 679, 986
AluBI AGCT 5 cut(s) 427, 445, 1195, 1361, 1368
AluI AGCT 5 cut(s) 427, 445, 1195, 1361, 1368
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 3 cut(s) 254, 583, 1170
AlwI GGATC 4 cut(s) 464, 1055, 1229, 1621
AlwNI CAGNNNCTG 1 cut(s) 830
Ama87I CYCGRG 2 cut(s) 515, 600
AoxI GGCC 4 cut(s) 135, 333, 469, 752
ApeKI GCWGC 1 cut(s) 1338
ApoI RAATTY 3 cut(s) 605, 1073, 1400
ArsI GACNNNNNNTTYG 4 cut(s) 1169, 1201, 1460, 1492
AspLEI GCGC 3 cut(s) 486, 488, 776
AspS9I GGNCC 4 cut(s) 136, 244, 752, 1442
AsuHPI GGTGA 4 cut(s) 76, 220, 289, 367
AvaI CYCGRG 2 cut(s) 515, 600
AvaII GGWCC 2 cut(s) 244, 1442
BanII GRGCYC 1 cut(s) 429
BarI GAAGNNNNNNTAC 2 cut(s) 900, 932
BauI CACGAG 1 cut(s) 567
BbsI GAAGAC 3 cut(s) 305, 324, 1199
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 1 cut(s) 1350
BccI CCATC 6 cut(s) 149, 158, 1150, 1196, 1268, 1493
BciT130I CCWGG 2 cut(s) 681, 988
BciVI GTATCC 2 cut(s) 877, 984
BclI TGATCA 2 cut(s) 66, 979
BcoDI GTCTC 3 cut(s) 254, 583, 1170
BcuI ACTAGT 1 cut(s) 919
BfaI CTAG 1 cut(s) 920
BfuAI ACCTGC 5 cut(s) 23, 31, 548, 1119, 1447
BfuI GTATCC 2 cut(s) 877, 984
BisI GCNGC 4 cut(s) 489, 492, 1263, 1339
BlsI GCNGC 4 cut(s) 490, 493, 1264, 1340
Bme1390I CCNGG 2 cut(s) 681, 988
Bme18I GGWCC 2 cut(s) 244, 1442
BmeRI GACNNNNNGTC 1 cut(s) 1226
BmeT110I CYCGRG 2 cut(s) 515, 600
BmgBI CACGTC 1 cut(s) 322
BmgT120I GGNCC 4 cut(s) 136, 244, 752, 1442
BmiI GGNNCC 3 cut(s) 138, 545, 726
BmrFI CCNGG 2 cut(s) 681, 988
BmsI GCATC 3 cut(s) 227, 394, 1249
BpiI GAAGAC 3 cut(s) 305, 324, 1199
BplI GAGNNNNNCTC 2 cut(s) 771, 803
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 1 cut(s) 1196
BpuEI CTTGAG 2 cut(s) 1196, 1341
BsaAI YACGTR 1 cut(s) 888
BsaI GGTCTC 2 cut(s) 254, 583
BsaJI CCNNGG 3 cut(s) 121, 986, 1263
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 145, 241
Bse118I RCCGGY 2 cut(s) 246, 254
Bse1I ACTGG 2 cut(s) 1546, 1556
Bse3DI GCAATG 3 cut(s) 1421, 1536, 1590
BseBI CCWGG 2 cut(s) 681, 988
BseDI CCNNGG 3 cut(s) 121, 986, 1263
BseGI GGATG 8 cut(s) 169, 174, 409, 982, 1207, 1273, 1435, 1504
BseLI CCNNNNNNNGG 3 cut(s) 124, 145, 241
BseMI GCAATG 3 cut(s) 1421, 1536, 1590
BseMII CTCAG 2 cut(s) 894, 1105
BseNI ACTGG 2 cut(s) 1546, 1556
BsePI GCGCGC 1 cut(s) 484
BseRI GAGGAG 4 cut(s) 340, 940, 1313, 1532
BseXI GCAGC 1 cut(s) 1350
Bsh1236I CGCG 4 cut(s) 123, 269, 486, 1265
BshFI GGCC 4 cut(s) 137, 335, 471, 754
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 2 cut(s) 515, 600
BsiSI CCGG 2 cut(s) 247, 255
BslI CCNNNNNNNGG 3 cut(s) 124, 145, 241
BsmAI GTCTC 3 cut(s) 254, 583, 1170
BsnI GGCC 4 cut(s) 137, 335, 471, 754
Bso31I GGTCTC 2 cut(s) 254, 583
BsoBI CYCGRG 2 cut(s) 515, 600
Bsp1286I GDGCHC 1 cut(s) 429
Bsp1407I TGTACA 1 cut(s) 17
Bsp143I GATC 8 cut(s) 58, 66, 339, 456, 979, 1047, 1234, 1626
BspACI CCGC 7 cut(s) 121, 123, 489, 492, 541, 1263, 1265
BspANI GGCC 4 cut(s) 137, 335, 471, 754
BspCNI CTCAG 2 cut(s) 895, 1104
BspFNI CGCG 4 cut(s) 123, 269, 486, 1265
BspHI TCATGA 2 cut(s) 91, 342
BspLI GGNNCC 3 cut(s) 138, 545, 726
BspMI ACCTGC 5 cut(s) 23, 31, 548, 1119, 1447
BspPI GGATC 4 cut(s) 464, 1055, 1229, 1621
BspQI GCTCTTC 1 cut(s) 1185
BspTNI GGTCTC 2 cut(s) 254, 583
BsrDI GCAATG 3 cut(s) 1421, 1536, 1590
BsrFI RCCGGY 2 cut(s) 246, 254
BsrGI TGTACA 1 cut(s) 17
BsrI ACTGG 2 cut(s) 1546, 1556
BssAI RCCGGY 2 cut(s) 246, 254
BssECI CCNNGG 3 cut(s) 121, 986, 1263
BssHII GCGCGC 1 cut(s) 484
BssMI GATC 8 cut(s) 58, 66, 339, 456, 979, 1047, 1234, 1626
BssSI CACGAG 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 567
Bst2UI CCWGG 2 cut(s) 681, 988
Bst4CI ACNGT 3 cut(s) 639, 964, 1295
Bst6I CTCTTC 1 cut(s) 1185
BstAUI TGTACA 1 cut(s) 17
BstBAI YACGTR 1 cut(s) 888
BstC8I GCNNGC 3 cut(s) 271, 443, 486
BstDEI CTNAG 4 cut(s) 903, 1091, 1196, 1598
BstDSI CCRYGG 2 cut(s) 121, 1263
BstF5I GGATG 8 cut(s) 169, 174, 409, 982, 1207, 1273, 1435, 1504
BstFNI CGCG 4 cut(s) 123, 269, 486, 1265
BstHHI GCGC 3 cut(s) 486, 488, 776
BstKTI GATC 8 cut(s) 61, 69, 342, 459, 982, 1050, 1237, 1629
BstMAI GTCTC 3 cut(s) 254, 583, 1170
BstMBI GATC 8 cut(s) 58, 66, 339, 456, 979, 1047, 1234, 1626
BstNI CCWGG 2 cut(s) 681, 988
BstSCI CCNGG 2 cut(s) 679, 986
BstUI CGCG 4 cut(s) 123, 269, 486, 1265
BstV1I GCAGC 1 cut(s) 1350
BstV2I GAAGAC 3 cut(s) 305, 324, 1199
BstXI CCANNNNNNTGG 1 cut(s) 573
BsuI GTATCC 2 cut(s) 877, 984
BsuRI GGCC 4 cut(s) 137, 335, 471, 754
BtgI CCRYGG 2 cut(s) 121, 1263
BtrI CACGTC 1 cut(s) 322
BtsCI GGATG 8 cut(s) 169, 174, 409, 982, 1207, 1273, 1435, 1504
BtsIMutI CAGTG 2 cut(s) 1230, 1286
BveI ACCTGC 5 cut(s) 23, 31, 548, 1119, 1447
Cac8I GCNNGC 3 cut(s) 271, 443, 486
CaiI CAGNNNCTG 1 cut(s) 830
CciI TCATGA 2 cut(s) 91, 342
CfoI GCGC 3 cut(s) 486, 488, 776
Cfr10I RCCGGY 2 cut(s) 246, 254
Cfr13I GGNCC 4 cut(s) 136, 244, 752, 1442
Cfr42I CCGCGG 2 cut(s) 124, 1266
CsiI ACCWGGT 1 cut(s) 679
Csp6I GTAC 3 cut(s) 18, 187, 1595
CviAII CATG 5 cut(s) 92, 343, 421, 573, 721
CviQI GTAC 3 cut(s) 18, 187, 1595
DdeI CTNAG 4 cut(s) 903, 1091, 1196, 1598
DpnI GATC 8 cut(s) 60, 68, 341, 458, 981, 1049, 1236, 1628
DpnII GATC 8 cut(s) 58, 66, 339, 456, 979, 1047, 1234, 1626
DraI TTTAAA 1 cut(s) 1525
DriI GACNNNNNGTC 1 cut(s) 1226
Eam1104I CTCTTC 1 cut(s) 1185
Eam1105I GACNNNNNGTC 1 cut(s) 1226
EarI CTCTTC 1 cut(s) 1185
Ecl136II GAGCTC 1 cut(s) 427
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 2 cut(s) 254, 583
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 2 cut(s) 244, 1442
Eco53kI GAGCTC 1 cut(s) 427
Eco57I CTGAAG 2 cut(s) 777, 852
Eco88I CYCGRG 2 cut(s) 515, 600
EcoICRI GAGCTC 1 cut(s) 427
EcoRII CCWGG 2 cut(s) 679, 986
EcoRV GATATC 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 429
FaeI CATG 5 cut(s) 95, 346, 424, 576, 724
FalI AAGNNNNNCTT 2 cut(s) 960, 992
FatI CATG 5 cut(s) 91, 342, 420, 572, 720
FauI CCCGC 1 cut(s) 116
FbaI TGATCA 2 cut(s) 66, 979
Fnu4HI GCNGC 4 cut(s) 489, 492, 1263, 1339
FokI GGATG 8 cut(s) 161, 176, 416, 969, 1214, 1280, 1442, 1511
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 4 cut(s) 489, 492, 1263, 1339
FspBI CTAG 1 cut(s) 920
GlaI GCGC 3 cut(s) 485, 487, 775
GluI GCNGC 4 cut(s) 489, 492, 1263, 1339
GsuI CTGGAG 1 cut(s) 711
HaeIII GGCC 4 cut(s) 137, 335, 471, 754
HapII CCGG 2 cut(s) 247, 255
HhaI GCGC 3 cut(s) 486, 488, 776
Hin1II CATG 5 cut(s) 95, 346, 424, 576, 724
Hin6I GCGC 3 cut(s) 484, 486, 774
HinP1I GCGC 3 cut(s) 484, 486, 774
HindIII AAGCTT 1 cut(s) 1359
HinfI GANTC 7 cut(s) 95, 225, 581, 690, 957, 1134, 1506
HpaII CCGG 2 cut(s) 247, 255
HphI GGTGA 4 cut(s) 76, 220, 289, 367
Hpy166II GTNNAC 7 cut(s) 20, 38, 244, 1059, 1321, 1486, 1636
Hpy188I TCNGA 8 cut(s) 687, 879, 904, 1014, 1040, 1094, 1139, 1631
Hpy188III TCNNGA 8 cut(s) 92, 343, 515, 588, 728, 1213, 1238, 1578
Hpy8I GTNNAC 7 cut(s) 20, 38, 244, 1059, 1321, 1486, 1636
Hpy99I CGWCG 3 cut(s) 115, 744, 1346
HpyAV CCTTC 5 cut(s) 274, 388, 830, 874, 1124
HpyCH4III ACNGT 3 cut(s) 639, 964, 1295
HpyCH4IV ACGT 4 cut(s) 321, 498, 742, 887
HpyCH4V TGCA 9 cut(s) 273, 407, 525, 557, 617, 635, 845, 1001, 1246
HpyF3I CTNAG 4 cut(s) 903, 1091, 1196, 1598
HpySE526I ACGT 4 cut(s) 321, 498, 742, 887
Hsp92II CATG 5 cut(s) 95, 346, 424, 576, 724
HspAI GCGC 3 cut(s) 484, 486, 774
Ksp22I TGATCA 2 cut(s) 66, 979
KspI CCGCGG 2 cut(s) 124, 1266
Kzo9I GATC 8 cut(s) 58, 66, 339, 456, 979, 1047, 1234, 1626
LguI GCTCTTC 1 cut(s) 1185
LmnI GCTCC 3 cut(s) 424, 730, 771
Lsp1109I GCAGC 1 cut(s) 1350
LweI GCATC 3 cut(s) 227, 394, 1249
MabI ACCWGGT 1 cut(s) 679
MaeI CTAG 1 cut(s) 920
MaeII ACGT 4 cut(s) 321, 498, 742, 887
MaeIII GTNAC 5 cut(s) 373, 460, 499, 863, 1228
MalI GATC 8 cut(s) 60, 68, 341, 458, 981, 1049, 1236, 1628
MboI GATC 8 cut(s) 58, 66, 339, 456, 979, 1047, 1234, 1626
MboII GAAGA 7 cut(s) 310, 329, 845, 980, 1093, 1199, 1202
MfeI CAATTG 1 cut(s) 648
MhlI GDGCHC 1 cut(s) 429
MlyI GAGTC 2 cut(s) 89, 951
MmeI TCCRAC 1 cut(s) 685
MseI TTAA 8 cut(s) 537, 641, 1043, 1077, 1253, 1412, 1524, 1613
MslI CAYNNNNRTG 3 cut(s) 146, 571, 1064
MspA1I CMGCKG 2 cut(s) 123, 1265
MspI CCGG 2 cut(s) 247, 255
MspR9I CCNGG 2 cut(s) 681, 988
MunI CAATTG 1 cut(s) 648
MvaI CCWGG 2 cut(s) 681, 988
MvnI CGCG 4 cut(s) 123, 269, 486, 1265
NdeII GATC 8 cut(s) 58, 66, 339, 456, 979, 1047, 1234, 1626
NlaIII CATG 5 cut(s) 95, 346, 424, 576, 724
NlaIV GGNNCC 3 cut(s) 138, 545, 726
NmeAIII GCCGAG 2 cut(s) 113, 361
NmuCI GTSAC 3 cut(s) 373, 460, 1228
PaeR7I CTCGAG 1 cut(s) 515
PagI TCATGA 2 cut(s) 91, 342
PaqCI CACCTGC 1 cut(s) 31
PauI GCGCGC 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 1185
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PfeI GAWTC 5 cut(s) 225, 581, 690, 1134, 1506
PkrI GCNGC 4 cut(s) 490, 493, 1264, 1340
PleI GAGTC 2 cut(s) 89, 951
PpsI GAGTC 2 cut(s) 89, 951
Ppu21I YACGTR 1 cut(s) 888
Psp124BI GAGCTC 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 679, 986
PspGI CCWGG 2 cut(s) 679, 986
PspN4I GGNNCC 3 cut(s) 138, 545, 726
PspPI GGNCC 4 cut(s) 136, 244, 752, 1442
PstNI CAGNNNCTG 1 cut(s) 830
PteI GCGCGC 1 cut(s) 484
RsaI GTAC 3 cut(s) 19, 188, 1596
RsaNI GTAC 3 cut(s) 18, 187, 1595
RseI CAYNNNNRTG 3 cut(s) 146, 571, 1064
SacI GAGCTC 1 cut(s) 429
SacII CCGCGG 2 cut(s) 124, 1266
SapI GCTCTTC 1 cut(s) 1185
SaqAI TTAA 8 cut(s) 537, 641, 1043, 1077, 1253, 1412, 1524, 1613
SatI GCNGC 4 cut(s) 489, 492, 1263, 1339
Sau3AI GATC 8 cut(s) 58, 66, 339, 456, 979, 1047, 1234, 1626
Sau96I GGNCC 4 cut(s) 136, 244, 752, 1442
SchI GAGTC 2 cut(s) 89, 951
ScrFI CCNGG 2 cut(s) 681, 988
SduI GDGCHC 1 cut(s) 429
SexAI ACCWGGT 1 cut(s) 679
SfaNI GCATC 3 cut(s) 227, 394, 1249
Sfr274I CTCGAG 1 cut(s) 515
Sfr303I CCGCGG 2 cut(s) 124, 1266
SgrBI CCGCGG 2 cut(s) 124, 1266
SinI GGWCC 2 cut(s) 244, 1442
SlaI CTCGAG 1 cut(s) 515
SmiMI CAYNNNNRTG 3 cut(s) 146, 571, 1064
SmlI CTYRAG 3 cut(s) 515, 1211, 1356
SmoI CTYRAG 3 cut(s) 515, 1211, 1356
SpeI ACTAGT 1 cut(s) 919
SsiI CCGC 7 cut(s) 121, 123, 489, 492, 541, 1263, 1265
SspI AATATT 1 cut(s) 1574
SspMI CTAG 1 cut(s) 920
SstI GAGCTC 1 cut(s) 429
StyD4I CCNGG 2 cut(s) 679, 986
TaaI ACNGT 3 cut(s) 639, 964, 1295
TaiI ACGT 4 cut(s) 324, 501, 745, 890
TaqI TCGA 7 cut(s) 113, 450, 516, 587, 1272, 1479, 1625
TatI WGTACW 3 cut(s) 17, 186, 1594
TauI GCSGC 3 cut(s) 491, 494, 1265
TfiI GAWTC 5 cut(s) 225, 581, 690, 1134, 1506
Tru1I TTAA 8 cut(s) 537, 641, 1043, 1077, 1253, 1412, 1524, 1613
Tru9I TTAA 8 cut(s) 537, 641, 1043, 1077, 1253, 1412, 1524, 1613
TscAI CASTG 2 cut(s) 1237, 1293
TseFI GTSAC 3 cut(s) 373, 460, 1228
TseI GCWGC 1 cut(s) 1338
Tsp45I GTSAC 3 cut(s) 373, 460, 1228
TspDTI ATGAA 6 cut(s) 80, 573, 690, 1082, 1134, 1550
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 2 cut(s) 1237, 1293
VpaK11BI GGWCC 2 cut(s) 244, 1442
XapI RAATTY 3 cut(s) 605, 1073, 1400
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XhoI CTCGAG 1 cut(s) 515
XspI CTAG 1 cut(s) 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.