AT2G32280

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015185
Physical Location & Seq
Forward (+)
13712928 .. 13715208
2281 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32280.2

Sequence Viewer

Length: 492 bp
ATGACAAAGATAGGAGGTATTCTTGTTTGTCTAGTCATTGTGGGCTTAGATGTAGCTGCTGCAATCCTCGGAATCCAAGCTGAAGTCGCCCAAAATCAGGTGAAGCACATGAGGCTTTGGTTATTTGAGTGCAGAGAGCCGAGCCAGGACGCGTTCAGGCTCGGTCTAGGTGCAGCCGCGATATTGGTAATGGCTCATGTCCTACTTAACTTGGTCGGAGGCTGTCTTTGTATTTGCTCTCAAGACGAGTTTCAAAGATCTTCTTCCACTAGACAAATCTCCATGGCTTGTCTTGTCCTCACTTGGATCGTATTCGCTGTTGGATTTGGGAGTATAGTGATTGGGACGATGTCGAACAGTAAGTCAAGATCCTCTTGTGGATTCACACATCACCATTTTCTCTCCATTGGAGGGATTTTGTGTTTTCTTCATGCCCTCTTCTGTGTTGCTTATTACGTTTCTGCCACTGCGGCCAAAGATGAAGCTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

163

Amino Acids

17.48

Weight (kDa)

8.11

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 146 3.1e-21 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32280 AT2G32280
fragaria_vesca FvH4_6g43740 FvH4_6g43740
malus_domestica MD09G1103300.v1.1 MD17G1091200.v1.1
prunus_persica Prupe.3G222400_v2.0.a1
pyrus_communis pycom09g02680 pycom17g08810
rosa_chinensis RchiOBHm_Chr2g0160471
rosa_laevigata RLG00000021216
rosa_multiflora Rmu_sc0002771.1_g000002
rosa_roxburghii Rroxscaffold_2G00089800
rosa_rugosa Rorug02G0483300
rosa_samantha Rh2DG570900
rosa_wichuraiana Rw2G045410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 152, 179
AciI CCGC 2 cut(s) 177, 470
AclWI GGATC 2 cut(s) 314, 363
AcoI YGGCCR 1 cut(s) 471
AcuI CTGAAG 1 cut(s) 102
AfiI CCNNNNNNNGG 2 cut(s) 97, 411
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 1 cut(s) 254
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 3 cut(s) 56, 80, 485
AluI AGCT 3 cut(s) 56, 80, 485
AlwI GGATC 2 cut(s) 314, 363
AoxI GGCC 1 cut(s) 471
ApeKI GCWGC 3 cut(s) 56, 59, 173
ArsI GACNNNNNNTTYG 2 cut(s) 347, 379
AsuHPI GGTGA 2 cut(s) 112, 383
BbvI GCAGC 3 cut(s) 43, 46, 185
BciT130I CCWGG 1 cut(s) 146
BfaI CTAG 3 cut(s) 32, 167, 270
BglI GCCNNNNNGGC 1 cut(s) 470
BglII AGATCT 1 cut(s) 257
BisI GCNGC 5 cut(s) 57, 60, 174, 177, 471
BlsI GCNGC 5 cut(s) 58, 61, 175, 178, 472
Bme1390I CCNGG 1 cut(s) 146
BmrFI CCNGG 1 cut(s) 146
BpuEI CTTGAG 1 cut(s) 225
BsaJI CCNNGG 2 cut(s) 67, 282
Bsc4I CCNNNNNNNGG 2 cut(s) 97, 411
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 2 cut(s) 67, 282
BseLI CCNNNNNNNGG 2 cut(s) 97, 411
BseXI GCAGC 3 cut(s) 43, 46, 185
BsgI GTGCAG 2 cut(s) 151, 192
Bsh1236I CGCG 2 cut(s) 152, 179
BshFI GGCC 1 cut(s) 473
BslFI GGGAC 1 cut(s) 358
BslI CCNNNNNNNGG 2 cut(s) 97, 411
BsmFI GGGAC 1 cut(s) 358
BsnI GGCC 1 cut(s) 473
Bsp143I GATC 3 cut(s) 257, 306, 368
Bsp19I CCATGG 1 cut(s) 282
BspACI CCGC 2 cut(s) 177, 470
BspANI GGCC 1 cut(s) 473
BspFNI CGCG 2 cut(s) 152, 179
BspPI GGATC 2 cut(s) 314, 363
BssECI CCNNGG 2 cut(s) 67, 282
BssMI GATC 3 cut(s) 257, 306, 368
BssT1I CCWWGG 1 cut(s) 282
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 359
Bst6I CTCTTC 1 cut(s) 443
BstDEI CTNAG 2 cut(s) 46, 486
BstDSI CCRYGG 1 cut(s) 282
BstFNI CGCG 2 cut(s) 152, 179
BstKTI GATC 3 cut(s) 260, 309, 371
BstMBI GATC 3 cut(s) 257, 306, 368
BstMWI GCNNNNNNNGC 3 cut(s) 86, 112, 470
BstNI CCWGG 1 cut(s) 146
BstSCI CCNGG 1 cut(s) 144
BstUI CGCG 2 cut(s) 152, 179
BstV1I GCAGC 3 cut(s) 43, 46, 185
BstX2I RGATCY 2 cut(s) 257, 368
BstYI RGATCY 2 cut(s) 257, 368
BsuRI GGCC 1 cut(s) 473
BtgI CCRYGG 1 cut(s) 282
BtsI GCAGTG 1 cut(s) 465
BtsIMutI CAGTG 1 cut(s) 465
CseI GACGC 1 cut(s) 158
CviAII CATG 4 cut(s) 109, 197, 283, 431
DdeI CTNAG 2 cut(s) 46, 486
DpnI GATC 3 cut(s) 259, 308, 370
DpnII GATC 3 cut(s) 257, 306, 368
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 443
EarI CTCTTC 1 cut(s) 443
Eco130I CCWWGG 1 cut(s) 282
Eco57I CTGAAG 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 282
FaeI CATG 4 cut(s) 112, 200, 286, 434
FaiI YATR 5 cut(s) 110, 198, 284, 335, 432
FalI AAGNNNNNCTT 4 cut(s) 247, 279, 358, 390
FaqI GGGAC 1 cut(s) 358
FatI CATG 4 cut(s) 108, 196, 282, 430
Fnu4HI GCNGC 5 cut(s) 57, 60, 174, 177, 471
Fsp4HI GCNGC 5 cut(s) 57, 60, 174, 177, 471
FspBI CTAG 3 cut(s) 32, 167, 270
GluI GCNGC 5 cut(s) 57, 60, 174, 177, 471
HaeIII GGCC 1 cut(s) 473
HgaI GACGC 1 cut(s) 158
Hin1II CATG 4 cut(s) 112, 200, 286, 434
HinfI GANTC 2 cut(s) 72, 381
HphI GGTGA 2 cut(s) 112, 383
Hpy188I TCNGA 2 cut(s) 71, 218
Hpy188III TCNNGA 2 cut(s) 242, 366
HpyCH4III ACNGT 1 cut(s) 359
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 3 cut(s) 62, 132, 173
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 112, 470
HpyF3I CTNAG 2 cut(s) 46, 486
HpySE526I ACGT 1 cut(s) 456
Hsp92II CATG 4 cut(s) 112, 200, 286, 434
Kzo9I GATC 3 cut(s) 257, 306, 368
LpnPI CCDG 4 cut(s) 83, 131, 142, 158
Lsp1109I GCAGC 3 cut(s) 43, 46, 185
MaeI CTAG 3 cut(s) 32, 167, 270
MaeII ACGT 1 cut(s) 456
MalI GATC 3 cut(s) 259, 308, 370
MboI GATC 3 cut(s) 257, 306, 368
MboII GAAGA 4 cut(s) 252, 255, 419, 430
MflI RGATCY 2 cut(s) 257, 368
MluI ACGCGT 1 cut(s) 150
MmeI TCCRAC 2 cut(s) 196, 301
MnlI CCTC 8 cut(s) 8, 77, 105, 212, 308, 382, 404, 446
MseI TTAA 1 cut(s) 207
MspR9I CCNGG 1 cut(s) 146
MvaI CCWGG 1 cut(s) 146
MvnI CGCG 2 cut(s) 152, 179
MwoI GCNNNNNNNGC 3 cut(s) 86, 112, 470
NcoI CCATGG 1 cut(s) 282
NdeII GATC 3 cut(s) 257, 306, 368
NlaIII CATG 4 cut(s) 112, 200, 286, 434
NmeAIII GCCGAG 1 cut(s) 165
PfeI GAWTC 2 cut(s) 72, 381
PflFI GACNNNGTC 1 cut(s) 349
PkrI GCNGC 5 cut(s) 58, 61, 175, 178, 472
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PsuI RGATCY 2 cut(s) 257, 368
PsyI GACNNNGTC 1 cut(s) 349
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 5 cut(s) 57, 60, 174, 177, 471
Sau3AI GATC 3 cut(s) 257, 306, 368
ScrFI CCNGG 1 cut(s) 146
SetI ASST 7 cut(s) 19, 58, 82, 102, 172, 459, 487
SmlI CTYRAG 1 cut(s) 240
SmoI CTYRAG 1 cut(s) 240
SsiI CCGC 2 cut(s) 177, 470
SspMI CTAG 3 cut(s) 32, 167, 270
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 1 cut(s) 282
TaaI ACNGT 1 cut(s) 359
TaiI ACGT 1 cut(s) 459
TaqI TCGA 1 cut(s) 353
TaqII GACCGA 1 cut(s) 152
TauI GCSGC 2 cut(s) 179, 473
TfiI GAWTC 2 cut(s) 72, 381
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 1 cut(s) 472
TseI GCWGC 3 cut(s) 56, 59, 173
TspDTI ATGAA 1 cut(s) 419
TspRI CASTG 1 cut(s) 472
Tth111I GACNNNGTC 1 cut(s) 349
XspI CTAG 3 cut(s) 32, 167, 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.