Rw2G045410

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015185
Physical Location & Seq
Reverse (-)
72333867 .. 72335448
1582 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G045410.1

Sequence Viewer

Length: 483 bp
ATGGTTAAAATAGGAGGCATCTTTGTCTGTCTCTTGATTGTGGCCATGGACATCGGAGCTGGGATTCTCGGCATCGAAGCTGAACTTGAACAAAACAAGGTGAAACAATTAAGGTTATGGATCTTTGAGTGTAGAGAGCCAAGCCATCCGGCATTCAAGCTAGGGTTAGGTGCAGCAGGACTTCTGGTTCTTGCTCATGTCATTGCTAACTTGCTTGGTGGATGCAACAATTGCATTTGCTCTCAAGAAGAGCTCCAAAAGGCTCCTCCTAATAGACAACTCTCACTCGCATGCCTCGTCTTCACCTGGATCATTCTGGCCGTTGGATTGTCGATGCTGGTGATAGGGACTTGGTCGAACCACAAGTCAAGAGCTTCATGTGGTTTCACACACCATCATTTTCTTTCCATCGGAGGCATCTTGTGCTTTGTTCATGGCCTGTTTTGTGTGGCATATTACATTACTACCACTTCGACCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

160

Amino Acids

17.32

Weight (kDa)

7.6

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 146 2e-22 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32280 AT2G32280
fragaria_vesca FvH4_6g43740 FvH4_6g43740
malus_domestica MD09G1103300.v1.1 MD17G1091200.v1.1
prunus_persica Prupe.3G222400_v2.0.a1
pyrus_communis pycom09g02680 pycom17g08810
rosa_chinensis RchiOBHm_Chr2g0160471
rosa_laevigata RLG00000021216
rosa_multiflora Rmu_sc0002771.1_g000002
rosa_roxburghii Rroxscaffold_2G00089800
rosa_rugosa Rorug02G0483300
rosa_samantha Rh2DG570900
rosa_wichuraiana Rw2G045410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 128, 317
AcoI YGGCCR 2 cut(s) 42, 318
AgsI TTSAA 2 cut(s) 89, 157
AjnI CCWGG 1 cut(s) 305
AloI GAACNNNNNNTCC 2 cut(s) 171, 203
AluBI AGCT 5 cut(s) 59, 80, 160, 253, 374
AluI AGCT 5 cut(s) 59, 80, 160, 253, 374
Alw21I GWGCWC 1 cut(s) 255
Alw26I GTCTC 1 cut(s) 35
AlwI GGATC 2 cut(s) 128, 317
AoxI GGCC 3 cut(s) 42, 318, 436
ApeKI GCWGC 1 cut(s) 173
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AsuHPI GGTGA 3 cut(s) 112, 295, 352
BalI TGGCCA 1 cut(s) 44
BanII GRGCYC 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 292
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 185
BccI CCATC 3 cut(s) 153, 402, 416
BceAI ACGGC 1 cut(s) 305
BciT130I CCWGG 1 cut(s) 307
BcoDI GTCTC 1 cut(s) 35
BfaI CTAG 1 cut(s) 161
BisI GCNGC 1 cut(s) 174
BlsI GCNGC 1 cut(s) 175
Bme1390I CCNGG 1 cut(s) 307
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 1 cut(s) 307
BmsI GCATC 5 cut(s) 27, 81, 212, 324, 426
BpiI GAAGAC 1 cut(s) 292
BpuEI CTTGAG 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 45
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 2 cut(s) 145, 227
BseMI GCAATG 1 cut(s) 201
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 1 cut(s) 185
BseYI CCCAGC 1 cut(s) 59
BsgI GTGCAG 1 cut(s) 192
Bsh1285I CGRYCG 1 cut(s) 477
BshFI GGCC 3 cut(s) 44, 320, 438
BsiEI CGRYCG 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 1 cut(s) 149
BslFI GGGAC 1 cut(s) 361
BsmAI GTCTC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 361
BsmI GAATGC 1 cut(s) 152
BsnI GGCC 3 cut(s) 44, 320, 438
Bsp1286I GDGCHC 1 cut(s) 255
Bsp143I GATC 2 cut(s) 120, 309
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 3 cut(s) 44, 320, 438
BspLI GGNNCC 1 cut(s) 264
BspPI GGATC 2 cut(s) 128, 317
BspQI GCTCTTC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 201
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 2 cut(s) 120, 309
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 307
Bst6I CTCTTC 1 cut(s) 243
BstAPI GCANNNNNTGC 2 cut(s) 231, 423
BstC8I GCNNGC 1 cut(s) 292
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 2 cut(s) 145, 227
BstKTI GATC 2 cut(s) 123, 312
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 2 cut(s) 120, 309
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 2 cut(s) 231, 423
BstNI CCWGG 1 cut(s) 307
BstNSI RCATGY 1 cut(s) 294
BstSCI CCNGG 1 cut(s) 305
BstV1I GCAGC 1 cut(s) 185
BstV2I GAAGAC 1 cut(s) 292
BstX2I RGATCY 1 cut(s) 120
BstYI RGATCY 1 cut(s) 120
BsuRI GGCC 3 cut(s) 44, 320, 438
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 2 cut(s) 145, 227
Cac8I GCNNGC 1 cut(s) 292
CviAII CATG 5 cut(s) 46, 197, 291, 378, 434
DpnI GATC 2 cut(s) 122, 311
DpnII GATC 2 cut(s) 120, 309
EaeI YGGCCR 2 cut(s) 42, 318
Eam1104I CTCTTC 1 cut(s) 243
EarI CTCTTC 1 cut(s) 243
Ecl136II GAGCTC 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 255
Eco53kI GAGCTC 1 cut(s) 253
EcoICRI GAGCTC 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 305
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 5 cut(s) 49, 200, 294, 381, 437
FaiI YATR 7 cut(s) 47, 118, 198, 292, 379, 435, 454
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FaqI GGGAC 1 cut(s) 361
FatI CATG 5 cut(s) 45, 196, 290, 377, 433
Fnu4HI GCNGC 1 cut(s) 174
FokI GGATG 2 cut(s) 132, 234
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 1 cut(s) 174
FspBI CTAG 1 cut(s) 161
GluI GCNGC 1 cut(s) 174
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 3 cut(s) 44, 320, 438
HapII CCGG 1 cut(s) 149
Hin1II CATG 5 cut(s) 49, 200, 294, 381, 437
HinfI GANTC 1 cut(s) 64
HpaII CCGG 1 cut(s) 149
HphI GGTGA 3 cut(s) 112, 295, 352
Hpy188I TCNGA 2 cut(s) 56, 413
Hpy188III TCNNGA 3 cut(s) 34, 245, 369
HpyCH4V TGCA 3 cut(s) 173, 225, 234
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 423
Hsp92II CATG 5 cut(s) 49, 200, 294, 381, 437
Kzo9I GATC 2 cut(s) 120, 309
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 3 cut(s) 56, 258, 268
LpnPI CCDG 9 cut(s) 45, 162, 162, 170, 292, 302, 319, 323, 452
Lsp1109I GCAGC 1 cut(s) 185
LweI GCATC 5 cut(s) 27, 81, 212, 324, 426
MaeI CTAG 1 cut(s) 161
MalI GATC 2 cut(s) 122, 311
MboI GATC 2 cut(s) 120, 309
MboII GAAGA 2 cut(s) 260, 292
MfeI CAATTG 1 cut(s) 229
MflI RGATCY 1 cut(s) 120
MhlI GDGCHC 1 cut(s) 255
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 2 cut(s) 107, 229
MluNI TGGCCA 1 cut(s) 44
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 4 cut(s) 8, 276, 305, 407
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MseI TTAA 2 cut(s) 6, 110
MslI CAYNNNNRTG 1 cut(s) 289
Msp20I TGGCCA 1 cut(s) 44
MspI CCGG 1 cut(s) 149
MspR9I CCNGG 1 cut(s) 307
MunI CAATTG 1 cut(s) 229
Mva1269I GAATGC 1 cut(s) 152
MvaI CCWGG 1 cut(s) 307
MwoI GCNNNNNNNGC 2 cut(s) 231, 423
NcoI CCATGG 1 cut(s) 45
NdeII GATC 2 cut(s) 120, 309
NlaIII CATG 5 cut(s) 49, 200, 294, 381, 437
NlaIV GGNNCC 1 cut(s) 264
NmeAIII GCCGAG 1 cut(s) 48
NspI RCATGY 1 cut(s) 294
PaeI GCATGC 1 cut(s) 294
PciSI GCTCTTC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PctI GAATGC 1 cut(s) 152
PfeI GAWTC 1 cut(s) 64
PflFI GACNNNGTC 1 cut(s) 352
PkrI GCNGC 1 cut(s) 175
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 305
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 1 cut(s) 264
PsuI RGATCY 1 cut(s) 120
PsyI GACNNNGTC 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 289
SacI GAGCTC 1 cut(s) 255
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 2 cut(s) 6, 110
SatI GCNGC 1 cut(s) 174
Sau3AI GATC 2 cut(s) 120, 309
ScrFI CCNGG 1 cut(s) 307
SduI GDGCHC 1 cut(s) 255
SetI ASST 9 cut(s) 61, 82, 102, 116, 162, 172, 255, 308, 376
SfaNI GCATC 5 cut(s) 27, 81, 212, 324, 426
SmiMI CAYNNNNRTG 1 cut(s) 289
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
SphI GCATGC 1 cut(s) 294
Sse9I AATT 2 cut(s) 107, 229
SspMI CTAG 1 cut(s) 161
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 305
StyI CCWWGG 1 cut(s) 45
TaqI TCGA 4 cut(s) 75, 332, 356, 473
TasI AATT 2 cut(s) 107, 229
TfiI GAWTC 1 cut(s) 64
Tru1I TTAA 2 cut(s) 6, 110
Tru9I TTAA 2 cut(s) 6, 110
TseI GCWGC 1 cut(s) 173
TspDTI ATGAA 2 cut(s) 366, 422
Tth111I GACNNNGTC 1 cut(s) 352
XceI RCATGY 1 cut(s) 294
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.