FvH4_6g43740

Protein of unknown function (DUF1218)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0015185
Physical Location & Seq
Reverse (-)
33797142 .. 33798500
1359 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43740.t2

Sequence Viewer

Length: 483 bp
ATGGTTAAAGTAGGAGGCGTCTTTGTCTGTCTCTTGATTGTTGCCATGGACATCGTAGCTGGGATCCTCGGCATCGAAGCTGAACTTGAACAAAACAAGGTGAAGCAATTAAGGTTGTGGATCTTTGAGTGTAGAGAGCCAAGCCATCCAGCATTCAAGCTAGGGCTAGGTGCAGCAGCACTTCTGGTTCTTGCCCATATCATTGCTAACTTGCTTGGTGGATGCAACAATTGCATTTGCTCTCAAGATGAGCTGCAAAAGGCTCCTCCTAACAGGCAACTCTCACTCGCATGCCTCGTCTTTACCTGGGTCATTCTAGCCGTTGGATTGTCAATGCTGGTGATAGGGACTTGGTCGAACCACAAGTCAAGAGCTTCGTGTGGATTCACACACCATCATTTTCTTTCCATTGGAGGCATCTTGTGCTTTGTTCATGGCCTCTTTTGTGTAGCGTATTATATTACTGCCACTTCTACCGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1218

Protein Analysis

161

Amino Acids

17.31

Weight (kDa)

7.6

Isoelectric Point (pI)

40.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1218 PF06749 58 - 146 1.7e-22 Protein of unknown function (DUF1218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32280 AT2G32280
fragaria_vesca FvH4_6g43740 FvH4_6g43740
malus_domestica MD09G1103300.v1.1 MD17G1091200.v1.1
prunus_persica Prupe.3G222400_v2.0.a1
pyrus_communis pycom09g02680 pycom17g08810
rosa_chinensis RchiOBHm_Chr2g0160471
rosa_laevigata RLG00000021216
rosa_multiflora Rmu_sc0002771.1_g000002
rosa_roxburghii Rroxscaffold_2G00089800
rosa_rugosa Rorug02G0483300
rosa_samantha Rh2DG570900
rosa_wichuraiana Rw2G045410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 58, 71, 128
AcyI GRCGYC 1 cut(s) 18
AgsI TTSAA 2 cut(s) 89, 157
AjnI CCWGG 1 cut(s) 305
AluBI AGCT 5 cut(s) 59, 80, 160, 253, 374
AluI AGCT 5 cut(s) 59, 80, 160, 253, 374
Alw26I GTCTC 1 cut(s) 35
AlwI GGATC 3 cut(s) 58, 71, 128
AoxI GGCC 1 cut(s) 436
ApeKI GCWGC 3 cut(s) 173, 176, 253
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AsuHPI GGTGA 2 cut(s) 112, 352
BamHI GGATCC 1 cut(s) 63
BbvI GCAGC 3 cut(s) 185, 188, 240
BccI CCATC 2 cut(s) 153, 402
BceAI ACGGC 1 cut(s) 305
BciT130I CCWGG 1 cut(s) 307
BcoDI GTCTC 1 cut(s) 35
BfaI CTAG 3 cut(s) 161, 167, 317
BisI GCNGC 3 cut(s) 174, 177, 254
BlsI GCNGC 3 cut(s) 175, 178, 255
Bme1390I CCNGG 1 cut(s) 307
BmiI GGNNCC 2 cut(s) 65, 264
BmrFI CCNGG 1 cut(s) 307
BmsI GCATC 3 cut(s) 81, 212, 426
BpuEI CTTGAG 1 cut(s) 228
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 3 cut(s) 45, 67, 306
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 1 cut(s) 307
BseDI CCNNGG 3 cut(s) 45, 67, 306
BseGI GGATG 2 cut(s) 145, 227
BseMI GCAATG 1 cut(s) 201
BseRI GAGGAG 1 cut(s) 255
BseXI GCAGC 3 cut(s) 185, 188, 240
BseYI CCCAGC 1 cut(s) 59
BsgI GTGCAG 1 cut(s) 192
BshFI GGCC 1 cut(s) 438
BslFI GGGAC 1 cut(s) 361
BsmAI GTCTC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 361
BsmI GAATGC 1 cut(s) 152
BsnI GGCC 1 cut(s) 438
Bsp143I GATC 2 cut(s) 63, 120
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 65, 264
BspPI GGATC 3 cut(s) 58, 71, 128
BsrDI GCAATG 1 cut(s) 201
BssECI CCNNGG 3 cut(s) 45, 67, 306
BssMI GATC 2 cut(s) 63, 120
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 1 cut(s) 45
Bst2UI CCWGG 1 cut(s) 307
BstACI GRCGYC 1 cut(s) 18
BstAPI GCANNNNNTGC 2 cut(s) 231, 423
BstC8I GCNNGC 1 cut(s) 292
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 2 cut(s) 145, 227
BstKTI GATC 2 cut(s) 66, 123
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 2 cut(s) 63, 120
BstMWI GCNNNNNNNGC 2 cut(s) 231, 423
BstNI CCWGG 1 cut(s) 307
BstNSI RCATGY 1 cut(s) 294
BstSCI CCNGG 1 cut(s) 305
BstV1I GCAGC 3 cut(s) 185, 188, 240
BstX2I RGATCY 2 cut(s) 63, 120
BstYI RGATCY 2 cut(s) 63, 120
BsuRI GGCC 1 cut(s) 438
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 2 cut(s) 145, 227
Cac8I GCNNGC 1 cut(s) 292
CseI GACGC 1 cut(s) 7
CviAII CATG 3 cut(s) 46, 291, 434
DpnI GATC 2 cut(s) 65, 122
DpnII GATC 2 cut(s) 63, 120
Eco130I CCWWGG 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 305
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 3 cut(s) 49, 294, 437
FaiI YATR 5 cut(s) 47, 198, 292, 435, 459
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FaqI GGGAC 1 cut(s) 361
FatI CATG 3 cut(s) 45, 290, 433
Fnu4HI GCNGC 3 cut(s) 174, 177, 254
FokI GGATG 2 cut(s) 132, 234
Fsp4HI GCNGC 3 cut(s) 174, 177, 254
FspBI CTAG 3 cut(s) 161, 167, 317
GluI GCNGC 3 cut(s) 174, 177, 254
GsaI CCCAGC 1 cut(s) 63
HaeIII GGCC 1 cut(s) 438
HgaI GACGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 3 cut(s) 49, 294, 437
HinfI GANTC 1 cut(s) 384
HphI GGTGA 2 cut(s) 112, 352
Hpy188III TCNNGA 3 cut(s) 34, 245, 369
HpyCH4V TGCA 4 cut(s) 173, 225, 234, 256
HpyF10VI GCNNNNNNNGC 2 cut(s) 231, 423
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 3 cut(s) 49, 294, 437
Kzo9I GATC 2 cut(s) 63, 120
LmnI GCTCC 1 cut(s) 268
LpnPI CCDG 7 cut(s) 45, 162, 170, 259, 292, 319, 323
Lsp1109I GCAGC 3 cut(s) 185, 188, 240
LweI GCATC 3 cut(s) 81, 212, 426
MaeI CTAG 3 cut(s) 161, 167, 317
MalI GATC 2 cut(s) 65, 122
MboI GATC 2 cut(s) 63, 120
MfeI CAATTG 1 cut(s) 229
MflI RGATCY 2 cut(s) 63, 120
MluCI AATT 2 cut(s) 107, 229
MmeI TCCRAC 1 cut(s) 304
MnlI CCTC 6 cut(s) 8, 77, 276, 305, 407, 449
MseI TTAA 2 cut(s) 6, 110
MslI CAYNNNNRTG 1 cut(s) 289
MspR9I CCNGG 1 cut(s) 307
MunI CAATTG 1 cut(s) 229
Mva1269I GAATGC 1 cut(s) 152
MvaI CCWGG 1 cut(s) 307
MwoI GCNNNNNNNGC 2 cut(s) 231, 423
NcoI CCATGG 1 cut(s) 45
NdeII GATC 2 cut(s) 63, 120
NlaIII CATG 3 cut(s) 49, 294, 437
NlaIV GGNNCC 2 cut(s) 65, 264
NmeAIII GCCGAG 1 cut(s) 48
NspI RCATGY 1 cut(s) 294
PaeI GCATGC 1 cut(s) 294
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PctI GAATGC 1 cut(s) 152
PfeI GAWTC 1 cut(s) 384
PflFI GACNNNGTC 1 cut(s) 352
PkrI GCNGC 3 cut(s) 175, 178, 255
Psp6I CCWGG 1 cut(s) 305
PspFI CCCAGC 1 cut(s) 59
PspGI CCWGG 1 cut(s) 305
PspN4I GGNNCC 2 cut(s) 65, 264
PsuI RGATCY 2 cut(s) 63, 120
PsyI GACNNNGTC 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 289
SaqAI TTAA 2 cut(s) 6, 110
SatI GCNGC 3 cut(s) 174, 177, 254
Sau3AI GATC 2 cut(s) 63, 120
ScrFI CCNGG 1 cut(s) 307
SetI ASST 9 cut(s) 61, 82, 102, 116, 162, 172, 255, 308, 376
SfaNI GCATC 3 cut(s) 81, 212, 426
SmiMI CAYNNNNRTG 1 cut(s) 289
SmlI CTYRAG 1 cut(s) 243
SmoI CTYRAG 1 cut(s) 243
SphI GCATGC 1 cut(s) 294
Sse9I AATT 2 cut(s) 107, 229
SspMI CTAG 3 cut(s) 161, 167, 317
StyD4I CCNGG 1 cut(s) 305
StyI CCWWGG 1 cut(s) 45
TaqI TCGA 2 cut(s) 75, 356
TasI AATT 2 cut(s) 107, 229
TfiI GAWTC 1 cut(s) 384
Tru1I TTAA 2 cut(s) 6, 110
Tru9I TTAA 2 cut(s) 6, 110
TseI GCWGC 3 cut(s) 173, 176, 253
TspDTI ATGAA 1 cut(s) 422
Tth111I GACNNNGTC 1 cut(s) 352
XceI RCATGY 1 cut(s) 294
XspI CTAG 3 cut(s) 161, 167, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.