AT2G32510
ERF Family

belongs to the protein kinase superfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012850
Physical Location & Seq
Reverse (-)
13798561 .. 13800099
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32510.1

Sequence Viewer

Length: 1119 bp
ATGGAATGGACTAGAGGAAGAATCCTAGGCCGAGGCTCTACAGCCACCGTATACGCCGCCGCAGGTCACAATTCAGATGAAATCCTCGCCGTTAAATCATCTGAGGTTCACCGCTCAGAGTTCTTGCAAAGAGAAGCCAAGATTCTCTCGTCGTTAAGTTCTCCTTACGTGATCGGATACAGAGGATCAGAAACCAAGAGAGAGTCAAACGGTGTCGTTATGTATAATCTTTTGATGGAGTACGCACCGTATGGAACGTTGACCGATGCGGCGGCGAAAGACGGGGGAAGAGTCGACGAGACTCGGGTAGTGAAGTACACGCGCGATATACTAAAAGGATTGGAGTATATACACTCGAAAGGAATCGTGCATTGTGATGTAAAGGGTAGTAACGTGGTTATTTCGGAGAAAGGCGAGGCCAAGATTGCGGATTTTGGTTGTGCGAAACGGGTTGACCCGGTGTTCGAGTCGCCGGTTATGGGAACGCCGGCTTTTATGGCTCCAGAGGTGGCGCGTGGAGAGAAACAGGGGAAGGAGAGTGATATATGGGCGGTTGGTTGTACGATGATAGAGATGGTTACGGGTTCTCCACCGTGGACGAAGGCAGATTCGAGGGAGGATCCGGTTTCGGTTCTTTATCGGGTCGGGTATTCGAGTGAAACTCCGGAGCTTCCTTGTTTGCTTGCGGAAGAAGCAAAAGATTTTTTGGAAAAGTGTTTGAAGAGGGAAGCAAATGAAAGATGGACAGCGACACAACTCCTAAACCATCCTTTTTTGACAACTAAACCAGACATTGAACCAGTTCTGGTTCCCGGTTTGATTTCAAACTCACCGACAAGTGTGACAGATCAAACGTTTTGGAGATCAGTAGAGGAAGAGGAAGAAGAAGAAACAGAGGAAATACAGAAGGATTCGAGAGATCTTGATCGTTTAAGCTTGTGGGGTTGTTACTCGGAGAGGATCGGACGGCTGAAGTGTGTTGGTGGTTTGGATGGGACCAGATGTGATATGGAGGGTGGGGATTGGATTATGGTGAGAGCGAGGTGTGAAGGAACAATGATTAGTGGGTCACAAAAGGAATTGATTATTAGTGAAAATGTATTGGTGGGGGAATTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009789 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010119 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048831 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090702 GO:0097305 GO:0097306 GO:0099120 GO:0099568 GO:0140096 GO:1900055 GO:1900057 GO:1901419 GO:1901421 GO:1901564 GO:1901700 GO:1901701 GO:1902456 GO:1902457 GO:1905623 GO:1905957 GO:1905959 GO:2000024 GO:2000026 GO:2000038
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

372

Amino Acids

41.23

Weight (kDa)

4.98

Isoelectric Point (pI)

46.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 259 1.2e-59 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 255 6.3e-34 Protein tyrosine and serine/threonine kinase
ABC1 PF03109 77 - 157 6.3e-06 ABC1 atypical kinase-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05100 AT2G32510
fragaria_vesca FvH4_6g42900
malus_domestica MD09G1111000.v1.1 MD17G1098800.v1.1
prunus_persica Prupe.3G215000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0158821
rosa_laevigata RLG00000021116
rosa_multiflora Rmu_co8475471.1_g000001
rosa_roxburghii Rroxscaffold_2G00090970
rosa_rugosa Rorug02G0472800
rosa_samantha Rh2AG538600 Rh2AG538700 Rh2BG551300 Rh2CG521900 Rh2CG522000 Rh2DG561100
rosa_wichuraiana Rw2G044580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 53
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 2 cut(s) 51, 294
AccII CGCG 3 cut(s) 322, 324, 514
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 8 cut(s) 57, 60, 112, 269, 272, 428, 551, 686
AclI AACGTT 2 cut(s) 257, 854
AclWI GGATC 4 cut(s) 193, 614, 627, 968
AcuI CTGAAG 1 cut(s) 992
AfaI GTAC 3 cut(s) 242, 317, 562
AfiI CCNNNNNNNGG 1 cut(s) 479
AgsI TTSAA 3 cut(s) 721, 797, 825
AluBI AGCT 2 cut(s) 670, 936
AluI AGCT 2 cut(s) 670, 936
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 4 cut(s) 193, 614, 627, 968
Ama87I CYCGRG 1 cut(s) 303
Aor13HI TCCGGA 1 cut(s) 664
AoxI GGCC 2 cut(s) 28, 417
ArsI GACNNNNNNTTYG 2 cut(s) 446, 478
Asp700I GAANNNNTTC 1 cut(s) 801
AspA2I CCTAGG 1 cut(s) 25
AspLEI GCGC 2 cut(s) 324, 514
AspS9I GGNCC 1 cut(s) 996
AsuC2I CCSGG 2 cut(s) 458, 813
AsuHPI GGTGA 3 cut(s) 101, 822, 1045
AvaI CYCGRG 1 cut(s) 303
AvaII GGWCC 1 cut(s) 996
AvrII CCTAGG 1 cut(s) 25
BamHI GGATCC 1 cut(s) 619
BccI CCATC 5 cut(s) 229, 568, 735, 774, 986
BceAI ACGGC 2 cut(s) 74, 983
BciVI GTATCC 1 cut(s) 170
BcnI CCSGG 2 cut(s) 458, 813
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 2 cut(s) 12, 26
BfmI CTRYAG 1 cut(s) 39
BfuAI ACCTGC 1 cut(s) 53
BfuI GTATCC 1 cut(s) 170
BglII AGATCT 1 cut(s) 919
BisI GCNGC 4 cut(s) 57, 60, 270, 273
BlnI CCTAGG 1 cut(s) 25
BlsI GCNGC 4 cut(s) 58, 61, 271, 274
Bme1390I CCNGG 2 cut(s) 458, 813
Bme18I GGWCC 1 cut(s) 996
BmeT110I CYCGRG 1 cut(s) 303
BmgT120I GGNCC 1 cut(s) 996
BmiI GGNNCC 4 cut(s) 501, 621, 810, 997
BmrFI CCNGG 2 cut(s) 458, 813
BmsI GCATC 1 cut(s) 256
BplI GAGNNNNNCTC 2 cut(s) 646, 678
BpmI CTGGAG 1 cut(s) 486
BpuMI CCSGG 2 cut(s) 458, 813
BsaAI YACGTR 1 cut(s) 169
BsaBI GATNNNNATC 1 cut(s) 924
BsaJI CCNNGG 3 cut(s) 25, 31, 593
BsaWI WCCGGW 2 cut(s) 622, 664
BsaXI ACNNNNNCTCC 2 cut(s) 571, 601
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse118I RCCGGY 2 cut(s) 472, 487
Bse1I ACTGG 1 cut(s) 800
Bse8I GATNNNNATC 1 cut(s) 924
BseAI TCCGGA 1 cut(s) 664
BseDI CCNNGG 3 cut(s) 25, 31, 593
BseGI GGATG 2 cut(s) 766, 997
BseJI GATNNNNATC 1 cut(s) 924
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMII CTCAG 2 cut(s) 93, 129
BseNI ACTGG 1 cut(s) 800
Bsh1236I CGCG 3 cut(s) 322, 324, 514
BshFI GGCC 2 cut(s) 30, 419
BsiHKCI CYCGRG 1 cut(s) 303
BsiSI CCGG 6 cut(s) 458, 473, 488, 623, 665, 813
BslFI GGGAC 1 cut(s) 1009
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 1009
BsnI GGCC 2 cut(s) 30, 419
BsoBI CYCGRG 1 cut(s) 303
Bsp13I TCCGGA 1 cut(s) 664
Bsp143I GATC 8 cut(s) 171, 185, 619, 847, 863, 919, 925, 960
BspACI CCGC 8 cut(s) 57, 60, 112, 269, 272, 428, 551, 686
BspANI GGCC 2 cut(s) 30, 419
BspCNI CTCAG 2 cut(s) 94, 128
BspEI TCCGGA 1 cut(s) 664
BspFNI CGCG 3 cut(s) 322, 324, 514
BspLI GGNNCC 4 cut(s) 501, 621, 810, 997
BspMI ACCTGC 1 cut(s) 53
BspPI GGATC 4 cut(s) 193, 614, 627, 968
BsrBI CCGCTC 1 cut(s) 114
BsrFI RCCGGY 2 cut(s) 472, 487
BsrI ACTGG 1 cut(s) 800
BssAI RCCGGY 2 cut(s) 472, 487
BssECI CCNNGG 3 cut(s) 25, 31, 593
BssMI GATC 8 cut(s) 171, 185, 619, 847, 863, 919, 925, 960
BssNAI GTATAC 1 cut(s) 52
BssT1I CCWWGG 1 cut(s) 25
Bst1107I GTATAC 1 cut(s) 52
Bst4CI ACNGT 4 cut(s) 49, 212, 249, 594
Bst6I CTCTTC 3 cut(s) 283, 716, 870
BstBAI YACGTR 1 cut(s) 169
BstC8I GCNNGC 2 cut(s) 489, 684
BstDEI CTNAG 2 cut(s) 102, 115
BstDSI CCRYGG 1 cut(s) 593
BstF5I GGATG 2 cut(s) 766, 997
BstFNI CGCG 3 cut(s) 322, 324, 514
BstHHI GCGC 2 cut(s) 324, 514
BstKTI GATC 8 cut(s) 174, 188, 622, 850, 866, 922, 928, 963
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 8 cut(s) 171, 185, 619, 847, 863, 919, 925, 960
BstMWI GCNNNNNNNGC 3 cut(s) 425, 497, 692
BstSCI CCNGG 2 cut(s) 456, 811
BstSFI CTRYAG 1 cut(s) 39
BstUI CGCG 3 cut(s) 322, 324, 514
BstX2I RGATCY 2 cut(s) 619, 919
BstYI RGATCY 2 cut(s) 619, 919
BstZ17I GTATAC 1 cut(s) 52
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 2 cut(s) 30, 419
BtgI CCRYGG 1 cut(s) 593
BtsCI GGATG 2 cut(s) 766, 997
BveI ACCTGC 1 cut(s) 53
Cac8I GCNNGC 2 cut(s) 489, 684
CfoI GCGC 2 cut(s) 324, 514
Cfr10I RCCGGY 2 cut(s) 472, 487
Cfr13I GGNCC 1 cut(s) 996
Csp6I GTAC 3 cut(s) 241, 316, 561
CviQI GTAC 3 cut(s) 241, 316, 561
DdeI CTNAG 2 cut(s) 102, 115
DpnI GATC 8 cut(s) 173, 187, 621, 849, 865, 921, 927, 962
DpnII GATC 8 cut(s) 171, 185, 619, 847, 863, 919, 925, 960
Eam1104I CTCTTC 3 cut(s) 283, 716, 870
EarI CTCTTC 3 cut(s) 283, 716, 870
Eco130I CCWWGG 1 cut(s) 25
Eco47I GGWCC 1 cut(s) 996
Eco57I CTGAAG 1 cut(s) 992
Eco88I CYCGRG 1 cut(s) 303
EcoT14I CCWWGG 1 cut(s) 25
ErhI CCWWGG 1 cut(s) 25
FalI AAGNNNNNCTT 2 cut(s) 148, 180
FaqI GGGAC 1 cut(s) 1009
FblI GTMKAC 2 cut(s) 51, 294
Fnu4HI GCNGC 4 cut(s) 57, 60, 270, 273
FokI GGATG 2 cut(s) 753, 1004
Fsp4HI GCNGC 4 cut(s) 57, 60, 270, 273
FspBI CTAG 2 cut(s) 12, 26
GlaI GCGC 2 cut(s) 323, 513
GluI GCNGC 4 cut(s) 57, 60, 270, 273
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 2 cut(s) 30, 419
HapII CCGG 6 cut(s) 458, 473, 488, 623, 665, 813
HhaI GCGC 2 cut(s) 324, 514
Hin6I GCGC 2 cut(s) 322, 512
HinP1I GCGC 2 cut(s) 322, 512
HincII GTYRAC 3 cut(s) 261, 295, 454
HindII GTYRAC 3 cut(s) 261, 295, 454
HindIII AAGCTT 1 cut(s) 934
HinfI GANTC 9 cut(s) 21, 142, 203, 291, 301, 363, 467, 608, 911
HpaII CCGG 6 cut(s) 458, 473, 488, 623, 665, 813
HphI GGTGA 3 cut(s) 101, 822, 1045
Hpy166II GTNNAC 7 cut(s) 52, 109, 261, 295, 318, 454, 597
Hpy188I TCNGA 8 cut(s) 76, 103, 118, 176, 190, 406, 955, 965
Hpy188III TCNNGA 4 cut(s) 503, 665, 915, 923
Hpy8I GTNNAC 7 cut(s) 52, 109, 261, 295, 318, 454, 597
Hpy99I CGWCG 2 cut(s) 154, 299
HpyAV CCTTC 4 cut(s) 526, 595, 901, 1043
HpyCH4III ACNGT 4 cut(s) 49, 212, 249, 594
HpyCH4IV ACGT 4 cut(s) 168, 257, 393, 854
HpyCH4V TGCA 2 cut(s) 127, 370
HpyF10VI GCNNNNNNNGC 3 cut(s) 425, 497, 692
HpyF3I CTNAG 2 cut(s) 102, 115
HpySE526I ACGT 4 cut(s) 168, 257, 393, 854
HspAI GCGC 2 cut(s) 322, 512
Kpn2I TCCGGA 1 cut(s) 664
KroI GCCGGC 1 cut(s) 487
KroNI GCCGGC 1 cut(s) 489
Kzo9I GATC 8 cut(s) 171, 185, 619, 847, 863, 919, 925, 960
LmnI GCTCC 2 cut(s) 505, 667
LweI GCATC 1 cut(s) 256
MaeI CTAG 2 cut(s) 12, 26
MaeII ACGT 4 cut(s) 168, 257, 393, 854
MaeIII GTNAC 6 cut(s) 65, 389, 577, 841, 947, 1068
MalI GATC 8 cut(s) 173, 187, 621, 849, 865, 921, 927, 962
MbiI CCGCTC 1 cut(s) 114
MboI GATC 8 cut(s) 171, 185, 619, 847, 863, 919, 925, 960
MboII GAAGA 8 cut(s) 30, 300, 701, 733, 887, 893, 896, 899
MflI RGATCY 2 cut(s) 619, 919
MluCI AATT 3 cut(s) 70, 1079, 1112
MlyI GAGTC 4 cut(s) 212, 295, 300, 476
MroI TCCGGA 1 cut(s) 664
MroNI GCCGGC 1 cut(s) 487
MroXI GAANNNNTTC 1 cut(s) 801
MseI TTAA 3 cut(s) 93, 155, 932
MslI CAYNNNNRTG 1 cut(s) 375
MspI CCGG 6 cut(s) 458, 473, 488, 623, 665, 813
MspR9I CCNGG 2 cut(s) 458, 813
MvnI CGCG 3 cut(s) 322, 324, 514
MwoI GCNNNNNNNGC 3 cut(s) 425, 497, 692
NaeI GCCGGC 1 cut(s) 489
NciI CCSGG 2 cut(s) 458, 813
NdeII GATC 8 cut(s) 171, 185, 619, 847, 863, 919, 925, 960
NgoMIV GCCGGC 1 cut(s) 487
NlaIV GGNNCC 4 cut(s) 501, 621, 810, 997
NmeAIII GCCGAG 1 cut(s) 56
NmuCI GTSAC 3 cut(s) 65, 841, 1068
PdiI GCCGGC 1 cut(s) 489
PdmI GAANNNNTTC 1 cut(s) 801
PfeI GAWTC 5 cut(s) 21, 142, 363, 608, 911
PkrI GCNGC 4 cut(s) 58, 61, 271, 274
PleI GAGTC 4 cut(s) 211, 295, 299, 475
PpsI GAGTC 4 cut(s) 211, 295, 299, 475
Ppu21I YACGTR 1 cut(s) 169
Psp1406I AACGTT 2 cut(s) 257, 854
PspN4I GGNNCC 4 cut(s) 501, 621, 810, 997
PspPI GGNCC 1 cut(s) 996
PsuI RGATCY 2 cut(s) 619, 919
RsaI GTAC 3 cut(s) 242, 317, 562
RsaNI GTAC 3 cut(s) 241, 316, 561
RseI CAYNNNNRTG 1 cut(s) 375
SalI GTCGAC 1 cut(s) 293
SaqAI TTAA 3 cut(s) 93, 155, 932
SatI GCNGC 4 cut(s) 57, 60, 270, 273
Sau3AI GATC 8 cut(s) 171, 185, 619, 847, 863, 919, 925, 960
Sau96I GGNCC 1 cut(s) 996
SchI GAGTC 4 cut(s) 212, 295, 300, 476
ScrFI CCNGG 2 cut(s) 458, 813
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 1 cut(s) 39
SinI GGWCC 1 cut(s) 996
SmiMI CAYNNNNRTG 1 cut(s) 375
Sse9I AATT 3 cut(s) 70, 1079, 1112
SsiI CCGC 8 cut(s) 57, 60, 112, 269, 272, 428, 551, 686
SspMI CTAG 2 cut(s) 12, 26
StyD4I CCNGG 2 cut(s) 456, 811
StyI CCWWGG 1 cut(s) 25
TaaI ACNGT 4 cut(s) 49, 212, 249, 594
TaiI ACGT 4 cut(s) 171, 260, 396, 857
TaqI TCGA 6 cut(s) 294, 356, 465, 611, 653, 914
TaqII GACCGA 1 cut(s) 278
TasI AATT 3 cut(s) 70, 1079, 1112
TatI WGTACW 1 cut(s) 315
TauI GCSGC 4 cut(s) 59, 62, 272, 275
TfiI GAWTC 5 cut(s) 21, 142, 363, 608, 911
Tru1I TTAA 3 cut(s) 93, 155, 932
Tru9I TTAA 3 cut(s) 93, 155, 932
TseFI GTSAC 3 cut(s) 65, 841, 1068
Tsp45I GTSAC 3 cut(s) 65, 841, 1068
TspDTI ATGAA 2 cut(s) 93, 750
VpaK11BI GGWCC 1 cut(s) 996
XcmI CCANNNNNNNNNTGG 1 cut(s) 1006
XmaJI CCTAGG 1 cut(s) 25
XmiI GTMKAC 2 cut(s) 51, 294
XmnI GAANNNNTTC 1 cut(s) 801
XspI CTAG 2 cut(s) 12, 26
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.