Rh2AG538600

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0012850
Physical Location & Seq
Forward (+)
76662907 .. 76663662
756 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG538600.1

Sequence Viewer

Length: 756 bp
ATGGACTGGACTAGAGGCCACACCGTTGGCCGTGGCTCCTCCGCCGCCGTCTCCATCGCCACCTCTTCTGGCTCCGGCTCCAAAATCTTTGCAGTCAAGTCTGCCGAGCTCTCCCAATCCGATTTCTTGCAAAGAGAGCAAAAAATTCTCTCTTCTTTGAGCAGTCCTCATGTGGTAAGCTATTTAGGACATGACATTACTAGAGAGAACAACAAGCTCATGTACAATATCTTCATGGAGTACGTGGCCGGCGGCACGATCATCGATGTAATTCGCAACCGTGGAGGCCAGTTTGAAGAATCAGCAATCGGGTTTCACACGAGGCAAATTGCCAAGGGGCTAGAGTACTTGCATTCAGTTGGATTAGTACATTGTGACATAAAAGGAAGGAACATCTTGGTTGGAGAAGAGGGTCCGAAAATTGCCGACTTTGGATGCGCCAGGTGGGCTGATCCTGCGGCTGAGGCAGTGCCAATTGGCGGCACGCCGATGTTTATGTCACCGGAGGTGGCGCGTGGGGAGGAGCAGGGTTTTCCTTGTGATGTTTGGGCTCTTGGGTGCACAGTTATCGAAATGGCTACCGGAGGATCAGTGCCGTGGCCTAATGCAGCTGACCCAGTAACTCTTCTTTACCGGATAGCATACTCCGGTGAGTCGCCGGAGATTCCAAGTTTTCTTTCAAATCAAGCAAGGGATTTCTTGGCAAAGTGTTTCATGAGGGACCCAAAAGAGAGGTGGACGGTTACACACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009789 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010119 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048831 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090702 GO:0097305 GO:0097306 GO:0099120 GO:0099568 GO:0140096 GO:1900055 GO:1900057 GO:1901419 GO:1901421 GO:1901564 GO:1901700 GO:1901701 GO:1902456 GO:1902457 GO:1905623 GO:1905957 GO:1905959 GO:2000024 GO:2000026 GO:2000038
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

251

Amino Acids

27.36

Weight (kDa)

5.81

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 250 1.4e-48 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 249 7.3e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05100 AT2G32510
fragaria_vesca FvH4_6g42900
malus_domestica MD09G1111000.v1.1 MD17G1098800.v1.1
prunus_persica Prupe.3G215000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0158821
rosa_laevigata RLG00000021116
rosa_multiflora Rmu_co8475471.1_g000001
rosa_roxburghii Rroxscaffold_2G00090970
rosa_rugosa Rorug02G0472800
rosa_samantha Rh2AG538600 Rh2AG538700 Rh2BG551300 Rh2CG521900 Rh2CG522000 Rh2DG561100
rosa_wichuraiana Rw2G044580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 514
AciI CCGC 5 cut(s) 42, 45, 252, 458, 480
AclWI GGATC 2 cut(s) 446, 595
AcoI YGGCCR 2 cut(s) 28, 246
AcsI RAATTY 1 cut(s) 144
AfaI GTAC 4 cut(s) 224, 242, 347, 369
AfiI CCNNNNNNNGG 1 cut(s) 479
AgsI TTSAA 2 cut(s) 296, 681
AjnI CCWGG 1 cut(s) 440
AjuI GAANNNNNNNTTGG 2 cut(s) 661, 693
AluBI AGCT 4 cut(s) 109, 180, 217, 611
AluI AGCT 4 cut(s) 109, 180, 217, 611
Alw21I GWGCWC 2 cut(s) 111, 563
Alw26I GTCTC 1 cut(s) 55
Alw44I GTGCAC 1 cut(s) 559
AlwI GGATC 2 cut(s) 446, 595
AoxI GGCC 5 cut(s) 16, 28, 246, 286, 599
ApaLI GTGCAC 1 cut(s) 559
ApeKI GCWGC 1 cut(s) 608
ApoI RAATTY 1 cut(s) 144
AspLEI GCGC 2 cut(s) 440, 514
AspS9I GGNCC 2 cut(s) 413, 721
AsuHPI GGTGA 2 cut(s) 492, 662
AvaII GGWCC 2 cut(s) 413, 721
BaeGI GKGCMC 1 cut(s) 563
BanII GRGCYC 2 cut(s) 111, 553
BarI GAAGNNNNNNTAC 2 cut(s) 215, 247
BauI CACGAG 1 cut(s) 319
Bbv12I GWGCWC 2 cut(s) 111, 563
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 1 cut(s) 620
BccI CCATC 1 cut(s) 62
BceAI ACGGC 3 cut(s) 15, 32, 580
BcgI CGANNNNNNTGC 4 cut(s) 244, 278, 550, 584
BciT130I CCWGG 1 cut(s) 442
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 3 cut(s) 12, 201, 341
BglI GCCNNNNNGGC 1 cut(s) 446
BisI GCNGC 5 cut(s) 45, 253, 459, 481, 609
BlsI GCNGC 5 cut(s) 46, 254, 460, 482, 610
BmcAI AGTACT 1 cut(s) 347
Bme1390I CCNGG 1 cut(s) 442
Bme18I GGWCC 2 cut(s) 413, 721
BmgT120I GGNCC 2 cut(s) 413, 721
BmiI GGNNCC 6 cut(s) 37, 73, 79, 414, 722, 723
BmrFI CCNGG 1 cut(s) 442
BmrI ACTGGG 1 cut(s) 611
BmsI GCATC 1 cut(s) 425
BmuI ACTGGG 1 cut(s) 611
BplI GAGNNNNNCTC 2 cut(s) 151, 183
Bpu10I CCTNAGC 1 cut(s) 462
Bsa29I ATCGAT 1 cut(s) 264
BsaAI YACGTR 1 cut(s) 244
BsaJI CCNNGG 4 cut(s) 31, 280, 333, 596
BsaWI WCCGGW 4 cut(s) 502, 581, 633, 647
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 1 cut(s) 479
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 3 cut(s) 11, 289, 617
BseBI CCWGG 1 cut(s) 442
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 4 cut(s) 31, 280, 333, 596
BseGI GGATG 1 cut(s) 440
BseLI CCNNNNNNNGG 1 cut(s) 479
BseMII CTCAG 1 cut(s) 453
BseNI ACTGG 3 cut(s) 11, 289, 617
BseRI GAGGAG 2 cut(s) 28, 536
BseSI GKGCMC 1 cut(s) 563
BseXI GCAGC 1 cut(s) 620
Bsh1236I CGCG 1 cut(s) 514
BshFI GGCC 5 cut(s) 18, 30, 248, 288, 601
BshVI ATCGAT 1 cut(s) 264
BsiHKAI GWGCWC 2 cut(s) 111, 563
BsiSI CCGG 7 cut(s) 75, 249, 503, 582, 634, 648, 659
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 1 cut(s) 479
BsmAI GTCTC 1 cut(s) 55
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 1 cut(s) 734
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 5 cut(s) 18, 30, 248, 288, 601
Bsp1286I GDGCHC 3 cut(s) 111, 553, 563
Bsp1407I TGTACA 1 cut(s) 222
Bsp143I GATC 3 cut(s) 258, 451, 587
BspACI CCGC 5 cut(s) 42, 45, 252, 458, 480
BspANI GGCC 5 cut(s) 18, 30, 248, 288, 601
BspCNI CTCAG 1 cut(s) 454
BspDI ATCGAT 1 cut(s) 264
BspFNI CGCG 1 cut(s) 514
BspHI TCATGA 1 cut(s) 714
BspLI GGNNCC 6 cut(s) 37, 73, 79, 414, 722, 723
BspPI GGATC 2 cut(s) 446, 595
BsrFI RCCGGY 1 cut(s) 248
BsrGI TGTACA 1 cut(s) 222
BsrI ACTGG 3 cut(s) 11, 289, 617
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 4 cut(s) 31, 280, 333, 596
BssMI GATC 3 cut(s) 258, 451, 587
BssSI CACGAG 1 cut(s) 319
BssT1I CCWWGG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 319
Bst2UI CCWGG 1 cut(s) 442
Bst4CI ACNGT 4 cut(s) 25, 281, 565, 742
Bst6I CTCTTC 4 cut(s) 70, 157, 402, 630
BstAUI TGTACA 1 cut(s) 222
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 2 cut(s) 250, 485
BstDEI CTNAG 1 cut(s) 462
BstDSI CCRYGG 3 cut(s) 31, 280, 596
BstF5I GGATG 1 cut(s) 440
BstFNI CGCG 1 cut(s) 514
BstHHI GCGC 2 cut(s) 440, 514
BstKTI GATC 3 cut(s) 261, 454, 590
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 3 cut(s) 258, 451, 587
BstMWI GCNNNNNNNGC 4 cut(s) 136, 446, 455, 464
BstNI CCWGG 1 cut(s) 442
BstSCI CCNGG 1 cut(s) 440
BstSLI GKGCMC 1 cut(s) 563
BstUI CGCG 1 cut(s) 514
BstV1I GCAGC 1 cut(s) 620
BstXI CCANNNNNNTGG 1 cut(s) 26
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 5 cut(s) 18, 30, 248, 288, 601
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 3 cut(s) 31, 280, 596
BtgZI GCGATG 1 cut(s) 40
BtsCI GGATG 1 cut(s) 440
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 2 cut(s) 474, 597
Cac8I GCNNGC 2 cut(s) 250, 485
CciI TCATGA 1 cut(s) 714
CfoI GCGC 2 cut(s) 440, 514
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 2 cut(s) 413, 721
ClaI ATCGAT 1 cut(s) 264
Csp6I GTAC 4 cut(s) 223, 241, 346, 368
CviAII CATG 5 cut(s) 170, 191, 220, 235, 715
CviQI GTAC 4 cut(s) 223, 241, 346, 368
DdeI CTNAG 1 cut(s) 462
DpnI GATC 3 cut(s) 260, 453, 589
DpnII GATC 3 cut(s) 258, 451, 587
EaeI YGGCCR 2 cut(s) 28, 246
Eam1104I CTCTTC 4 cut(s) 70, 157, 402, 630
EarI CTCTTC 4 cut(s) 70, 157, 402, 630
EciI GGCGGA 1 cut(s) 31
Ecl136II GAGCTC 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 333
Eco24I GRGCYC 2 cut(s) 111, 553
Eco47I GGWCC 2 cut(s) 413, 721
Eco53kI GAGCTC 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 721
EcoRII CCWGG 1 cut(s) 440
EcoT14I CCWWGG 1 cut(s) 333
EcoT38I GRGCYC 2 cut(s) 111, 553
ErhI CCWWGG 1 cut(s) 333
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 5 cut(s) 173, 194, 223, 238, 718
FaiI YATR 8 cut(s) 171, 192, 221, 236, 380, 497, 643, 716
FaqI GGGAC 1 cut(s) 734
FatI CATG 5 cut(s) 169, 190, 219, 234, 714
Fnu4HI GCNGC 5 cut(s) 45, 253, 459, 481, 609
FokI GGATG 1 cut(s) 447
FriOI GRGCYC 2 cut(s) 111, 553
Fsp4HI GCNGC 5 cut(s) 45, 253, 459, 481, 609
FspBI CTAG 3 cut(s) 12, 201, 341
GlaI GCGC 2 cut(s) 439, 513
GluI GCNGC 5 cut(s) 45, 253, 459, 481, 609
HaeIII GGCC 5 cut(s) 18, 30, 248, 288, 601
HapII CCGG 7 cut(s) 75, 249, 503, 582, 634, 648, 659
HhaI GCGC 2 cut(s) 440, 514
Hin1II CATG 5 cut(s) 173, 194, 223, 238, 718
Hin6I GCGC 2 cut(s) 438, 512
HinP1I GCGC 2 cut(s) 438, 512
HinfI GANTC 3 cut(s) 299, 653, 664
HpaII CCGG 7 cut(s) 75, 249, 503, 582, 634, 648, 659
HphI GGTGA 2 cut(s) 492, 662
Hpy166II GTNNAC 2 cut(s) 561, 738
Hpy188I TCNGA 2 cut(s) 121, 417
Hpy188III TCNNGA 1 cut(s) 715
Hpy8I GTNNAC 2 cut(s) 561, 738
HpyAV CCTTC 1 cut(s) 381
HpyCH4III ACNGT 4 cut(s) 25, 281, 565, 742
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 5 cut(s) 92, 130, 352, 561, 608
HpyF10VI GCNNNNNNNGC 4 cut(s) 136, 446, 455, 464
HpyF3I CTNAG 1 cut(s) 462
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 5 cut(s) 173, 194, 223, 238, 718
HspAI GCGC 2 cut(s) 438, 512
KflI GGGWCCC 1 cut(s) 721
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Kzo9I GATC 3 cut(s) 258, 451, 587
LmnI GCTCC 4 cut(s) 41, 77, 83, 523
Lsp1109I GCAGC 1 cut(s) 620
LweI GCATC 1 cut(s) 425
MaeI CTAG 3 cut(s) 12, 201, 341
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 4 cut(s) 374, 498, 619, 742
MalI GATC 3 cut(s) 260, 453, 589
MboI GATC 3 cut(s) 258, 451, 587
MboII GAAGA 6 cut(s) 57, 144, 223, 308, 419, 617
MfeI CAATTG 1 cut(s) 474
MhlI GDGCHC 3 cut(s) 111, 553, 563
MluCI AATT 5 cut(s) 144, 270, 327, 420, 474
MlyI GAGTC 1 cut(s) 662
MmeI TCCRAC 2 cut(s) 340, 382
MroNI GCCGGC 1 cut(s) 248
MslI CAYNNNNRTG 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 7 cut(s) 75, 249, 503, 582, 634, 648, 659
MspR9I CCNGG 1 cut(s) 442
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 1 cut(s) 442
MvnI CGCG 1 cut(s) 514
MwoI GCNNNNNNNGC 4 cut(s) 136, 446, 455, 464
NaeI GCCGGC 1 cut(s) 250
NdeII GATC 3 cut(s) 258, 451, 587
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 5 cut(s) 173, 194, 223, 238, 718
NlaIV GGNNCC 6 cut(s) 37, 73, 79, 414, 722, 723
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 2 cut(s) 374, 498
PagI TCATGA 1 cut(s) 714
PctI GAATGC 1 cut(s) 352
PdiI GCCGGC 1 cut(s) 250
PfeI GAWTC 2 cut(s) 299, 664
PkrI GCNGC 5 cut(s) 46, 254, 460, 482, 610
PleI GAGTC 1 cut(s) 661
PpsI GAGTC 1 cut(s) 661
Ppu21I YACGTR 1 cut(s) 244
PpuMI RGGWCCY 1 cut(s) 721
Psp124BI GAGCTC 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 721
Psp6I CCWGG 1 cut(s) 440
PspGI CCWGG 1 cut(s) 440
PspN4I GGNNCC 6 cut(s) 37, 73, 79, 414, 722, 723
PspPI GGNCC 2 cut(s) 413, 721
PspPPI RGGWCCY 1 cut(s) 721
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 4 cut(s) 224, 242, 347, 369
RsaNI GTAC 4 cut(s) 223, 241, 346, 368
RseI CAYNNNNRTG 1 cut(s) 488
SacI GAGCTC 1 cut(s) 111
SatI GCNGC 5 cut(s) 45, 253, 459, 481, 609
Sau3AI GATC 3 cut(s) 258, 451, 587
Sau96I GGNCC 2 cut(s) 413, 721
ScaI AGTACT 1 cut(s) 347
SchI GAGTC 1 cut(s) 662
ScrFI CCNGG 1 cut(s) 442
SduI GDGCHC 3 cut(s) 111, 553, 563
SetI ASST 9 cut(s) 65, 111, 182, 219, 246, 446, 510, 613, 737
SfaNI GCATC 1 cut(s) 425
SinI GGWCC 2 cut(s) 413, 721
SmiMI CAYNNNNRTG 1 cut(s) 488
Sse9I AATT 5 cut(s) 144, 270, 327, 420, 474
SsiI CCGC 5 cut(s) 42, 45, 252, 458, 480
SspMI CTAG 3 cut(s) 12, 201, 341
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 440
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 4 cut(s) 25, 281, 565, 742
TaiI ACGT 1 cut(s) 246
TaqI TCGA 2 cut(s) 264, 570
TasI AATT 5 cut(s) 144, 270, 327, 420, 474
TatI WGTACW 3 cut(s) 222, 345, 367
TauI GCSGC 4 cut(s) 47, 255, 461, 483
TfiI GAWTC 2 cut(s) 299, 664
TscAI CASTG 2 cut(s) 474, 597
TseFI GTSAC 2 cut(s) 374, 498
TseI GCWGC 1 cut(s) 608
Tsp45I GTSAC 2 cut(s) 374, 498
TspDTI ATGAA 2 cut(s) 223, 703
TspRI CASTG 2 cut(s) 474, 597
VneI GTGCAC 1 cut(s) 559
VpaK11BI GGWCC 2 cut(s) 413, 721
XapI RAATTY 1 cut(s) 144
XcmI CCANNNNNNNNNTGG 1 cut(s) 732
XspI CTAG 3 cut(s) 12, 201, 341
ZrmI AGTACT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.