MD09G1111000.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012850
Physical Location & Seq
Reverse (-)
8251575 .. 8252849
1275 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1111000.v1.1.491

Sequence Viewer

Length: 1275 bp
ATGGACTGGACAAGAGGCTGCACCATTGGCCAAGGCTCATCAGCCACCGTCTCCTTAGCCACTTCTCGCCACTCCGGCGACAAGTTTGCCGTCAAGTCCGCTGAGATGTCCCATTCCCAGTTCTTGCAAAGGGAGCAGAAAATTTTCTCTGTTTTGAGCAGTCCACATATAGTAAGCTACATGGGGCATGACATTACTAATGAGAACAACAAGCTCGTGTACAATCTTCTCATGGAGTACGTGCCCGGCGGCACGATCATCGATGCAATTCGCAGCCGCGGAGGCCAAGTTGACGAATCAATAATCAGAAACTACACGAGGGGCATGGTGAAAGGGCTAGACTACTTGCATTCACTTGGGTTGGTACATTGTGACATAAAAGGCAGGAACATCTTGGTTGGTGAAGACGGTCCAAAAATTGCAGATTTTGGATGTGCTAGGTGGGCTAATCCGGCAGCAGCTTCCGTTGCCACAATTGGCGGCACGCCAATGTTTATGGCGCCAGAGGTGGCACGCGGAGAAGAGCAGGGGTTCGCGTGCGACGTATGGGCTCTCGGGTGTACAATTATCGAAATGGCTACCGGAGGATCAGCGCCGTGGCCTAACGCAGCTGACCCAGTAACTGTTCTTTATCAAATTGCATACTCTAATGAGCTGCCTGAGATTCCAAGCTTCCTCTCAGATCAAGCAAAGCACTTTTTGGAAAGGTGCTTACGGAGGGACCCAACAGATCGTTGGACGGCTAGTCAGCTTCTAAAGCATTCATTTCTTGGGGAGGAAGAAGAAGAAAAAGTTTTAATTAATTTTTCTAGCAGTACAAAACAAGTTCAAGGATCCACTTCATTTTCTCCAACAAGTATTCTCGATCAAGATGTATGGAATTCTTTTGGGGAATCAGAGAATTCGGGCAGTCTTGATCAAAACCCGAGTTTCGAAAACTCCTCTGATGATCAGAGGATCAGACGGTTGTCATTGTTTTCAGGGGACCCCAAGTGGAGTTGGGATGATGAGACTTGGATCGCAGTTAGAGGGAATGATTGTGATGAAAGCAACAGCATCGAGGATGATGCTGAAGCTGTAGGTAGTGAGGAGGATTTGGATTTAAATTTCTCAGATTGCAATAATCTTAGTTGTAGAATTAATGTGGATATTTTAGAGGCCTGCAAAAATGGTAGGAGAAATAGTAGTAGTGTAGGTTGGAGCAATCTCCTTCTTGAGAGGGACAGACTCGACAGAGACAATTTGTTGCTTCCTTCAGTTTCAAGCTTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009789 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010119 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048831 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090702 GO:0097305 GO:0097306 GO:0099120 GO:0099568 GO:0140096 GO:1900055 GO:1900057 GO:1901419 GO:1901421 GO:1901564 GO:1901700 GO:1901701 GO:1902456 GO:1902457 GO:1905623 GO:1905957 GO:1905959 GO:2000024 GO:2000026 GO:2000038
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

425

Amino Acids

46.86

Weight (kDa)

4.67

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 6 - 257 4.2e-55 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 7 - 253 4.4e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05100 AT2G32510
fragaria_vesca FvH4_6g42900
malus_domestica MD09G1111000.v1.1 MD17G1098800.v1.1
prunus_persica Prupe.3G215000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0158821
rosa_laevigata RLG00000021116
rosa_multiflora Rmu_co8475471.1_g000001
rosa_roxburghii Rroxscaffold_2G00090970
rosa_rugosa Rorug02G0472800
rosa_samantha Rh2AG538600 Rh2AG538700 Rh2BG551300 Rh2CG521900 Rh2CG522000 Rh2DG561100
rosa_wichuraiana Rw2G044580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 499
AccII CGCG 3 cut(s) 279, 516, 536
AciI CCGC 6 cut(s) 99, 249, 277, 279, 480, 516
AclWI GGATC 5 cut(s) 595, 828, 841, 965, 1025
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 4 cut(s) 141, 880, 901, 1105
AcuI CTGAAG 2 cut(s) 1092, 1239
AcyI GRCGYC 1 cut(s) 500
AfaI GTAC 5 cut(s) 221, 239, 366, 562, 817
AgsI TTSAA 2 cut(s) 830, 1263
AhdI GACNNNNNGTC 1 cut(s) 744
AluBI AGCT 9 cut(s) 177, 214, 461, 611, 655, 672, 751, 1076, 1266
AluI AGCT 9 cut(s) 177, 214, 461, 611, 655, 672, 751, 1076, 1266
Alw26I GTCTC 3 cut(s) 55, 1004, 1230
AlwI GGATC 5 cut(s) 595, 828, 841, 965, 1025
AlwNI CAGNNNCTG 1 cut(s) 623
Ama87I CYCGRG 2 cut(s) 554, 925
AoxI GGCC 4 cut(s) 28, 283, 599, 1158
ApeKI GCWGC 6 cut(s) 18, 273, 455, 458, 608, 655
ApoI RAATTY 4 cut(s) 141, 880, 901, 1105
AseI ATTAAT 2 cut(s) 801, 1140
Asp700I GAANNNNTTC 1 cut(s) 143
AspLEI GCGC 2 cut(s) 502, 595
AspS9I GGNCC 3 cut(s) 410, 721, 985
AsuC2I CCSGG 1 cut(s) 246
AsuHPI GGTGA 2 cut(s) 340, 413
AsuII TTCGAA 1 cut(s) 933
AvaI CYCGRG 2 cut(s) 554, 925
AvaII GGWCC 3 cut(s) 410, 721, 985
BaeGI GKGCMC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 30
BamHI GGATCC 1 cut(s) 833
BanI GGYRCC 1 cut(s) 499
BanII GRGCYC 1 cut(s) 553
BauI CACGAG 2 cut(s) 215, 316
BbsI GAAGAC 1 cut(s) 411
BbvI GCAGC 6 cut(s) 5, 285, 467, 470, 620, 642
BceAI ACGGC 3 cut(s) 74, 580, 756
BcgI CGANNNNNNTGC 8 cut(s) 68, 102, 241, 275, 1039, 1049, 1073, 1083
BclI TGATCA 2 cut(s) 916, 949
BcnI CCSGG 1 cut(s) 246
BcoDI GTCTC 3 cut(s) 55, 1004, 1230
BfaI CTAG 4 cut(s) 338, 438, 744, 810
BfmI CTRYAG 1 cut(s) 1077
BfoI RGCGCY 2 cut(s) 503, 596
BglI GCCNNNNNGGC 2 cut(s) 75, 282
BisI GCNGC 9 cut(s) 19, 250, 274, 277, 456, 459, 481, 609, 656
BlsI GCNGC 9 cut(s) 20, 251, 275, 278, 457, 460, 482, 610, 657
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 3 cut(s) 410, 721, 985
BmeRI GACNNNNNGTC 1 cut(s) 744
BmeT110I CYCGRG 2 cut(s) 554, 925
BmgT120I GGNCC 3 cut(s) 410, 721, 985
BmiI GGNNCC 6 cut(s) 501, 722, 723, 835, 986, 987
BmrFI CCNGG 1 cut(s) 246
BmrI ACTGGG 2 cut(s) 112, 611
BmsI GCATC 3 cut(s) 253, 1057, 1065
BmuI ACTGGG 2 cut(s) 112, 611
BoxI GACNNNNGTC 1 cut(s) 967
BpiI GAAGAC 1 cut(s) 411
Bpu10I CCTNAGC 1 cut(s) 55
Bpu14I TTCGAA 1 cut(s) 933
BpuEI CTTGAG 1 cut(s) 1235
BpuMI CCSGG 1 cut(s) 246
Bsa29I ATCGAT 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 241
BsaHI GRCGYC 1 cut(s) 500
BsaJI CCNNGG 3 cut(s) 31, 277, 596
BsaWI WCCGGW 1 cut(s) 581
BsaXI ACNNNNNCTCC 4 cut(s) 273, 303, 1168, 1198
Bse1I ACTGG 3 cut(s) 11, 118, 617
BseCI ATCGAT 1 cut(s) 261
BseDI CCNNGG 3 cut(s) 31, 277, 596
BseGI GGATG 3 cut(s) 437, 1009, 1069
BseMII CTCAG 4 cut(s) 93, 651, 693, 1125
BseNI ACTGG 3 cut(s) 11, 118, 617
BseRI GAGGAG 2 cut(s) 931, 1103
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 6 cut(s) 5, 285, 467, 470, 620, 642
BsgI GTGCAG 1 cut(s) 4
Bsh1236I CGCG 3 cut(s) 279, 516, 536
BshFI GGCC 4 cut(s) 30, 285, 601, 1160
BshNI GGYRCC 1 cut(s) 499
BshVI ATCGAT 1 cut(s) 261
BsiHKCI CYCGRG 2 cut(s) 554, 925
BsiSI CCGG 4 cut(s) 75, 246, 452, 582
BslFI GGGAC 4 cut(s) 94, 734, 998, 1235
BsmAI GTCTC 3 cut(s) 55, 1004, 1230
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 4 cut(s) 94, 734, 998, 1235
BsmI GAATGC 2 cut(s) 349, 760
BsnI GGCC 4 cut(s) 30, 285, 601, 1160
BsoBI CYCGRG 2 cut(s) 554, 925
Bsp119I TTCGAA 1 cut(s) 933
Bsp1286I GDGCHC 2 cut(s) 246, 553
Bsp1407I TGTACA 2 cut(s) 219, 560
BspACI CCGC 6 cut(s) 99, 249, 277, 279, 480, 516
BspANI GGCC 4 cut(s) 30, 285, 601, 1160
BspCNI CTCAG 4 cut(s) 94, 652, 692, 1124
BspDI ATCGAT 1 cut(s) 261
BspFNI CGCG 3 cut(s) 279, 516, 536
BspLI GGNNCC 6 cut(s) 501, 722, 723, 835, 986, 987
BspPI GGATC 5 cut(s) 595, 828, 841, 965, 1025
BspQI GCTCTTC 1 cut(s) 516
BspT104I TTCGAA 1 cut(s) 933
BspT107I GGYRCC 1 cut(s) 499
BsrGI TGTACA 2 cut(s) 219, 560
BsrI ACTGG 3 cut(s) 11, 118, 617
BssECI CCNNGG 3 cut(s) 31, 277, 596
BssNI GRCGYC 1 cut(s) 500
BssSI CACGAG 2 cut(s) 215, 316
BssT1I CCWWGG 1 cut(s) 31
Bst2BI CACGAG 2 cut(s) 215, 316
Bst4CI ACNGT 4 cut(s) 49, 410, 625, 966
Bst6I CTCTTC 1 cut(s) 516
BstACI GRCGYC 1 cut(s) 500
BstAUI TGTACA 2 cut(s) 219, 560
BstBAI YACGTR 1 cut(s) 241
BstBI TTCGAA 1 cut(s) 933
BstC8I GCNNGC 4 cut(s) 485, 514, 538, 1162
BstDEI CTNAG 6 cut(s) 55, 102, 660, 679, 1111, 1127
BstDSI CCRYGG 2 cut(s) 277, 596
BstF5I GGATG 3 cut(s) 437, 1009, 1069
BstFNI CGCG 3 cut(s) 279, 516, 536
BstH2I RGCGCY 2 cut(s) 503, 596
BstHHI GCGC 2 cut(s) 502, 595
BstMAI GTCTC 3 cut(s) 55, 1004, 1230
BstMWI GCNNNNNNNGC 8 cut(s) 27, 75, 133, 282, 443, 452, 467, 757
BstPAI GACNNNNGTC 1 cut(s) 967
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 1 cut(s) 1077
BstSLI GKGCMC 1 cut(s) 246
BstUI CGCG 3 cut(s) 279, 516, 536
BstV1I GCAGC 6 cut(s) 5, 285, 467, 470, 620, 642
BstV2I GAAGAC 1 cut(s) 411
BstX2I RGATCY 1 cut(s) 833
BstYI RGATCY 1 cut(s) 833
Bsu15I ATCGAT 1 cut(s) 261
BsuRI GGCC 4 cut(s) 30, 285, 601, 1160
BsuTUI ATCGAT 1 cut(s) 261
BtgI CCRYGG 2 cut(s) 277, 596
BtsCI GGATG 3 cut(s) 437, 1009, 1069
Cac8I GCNNGC 4 cut(s) 485, 514, 538, 1162
CaiI CAGNNNCTG 1 cut(s) 623
CfoI GCGC 2 cut(s) 502, 595
Cfr13I GGNCC 3 cut(s) 410, 721, 985
Cfr42I CCGCGG 1 cut(s) 280
ClaI ATCGAT 1 cut(s) 261
Csp6I GTAC 5 cut(s) 220, 238, 365, 561, 816
CviAII CATG 4 cut(s) 181, 188, 232, 325
CviQI GTAC 5 cut(s) 220, 238, 365, 561, 816
DdeI CTNAG 6 cut(s) 55, 102, 660, 679, 1111, 1127
DinI GGCGCC 1 cut(s) 501
DraI TTTAAA 1 cut(s) 1104
DriI GACNNNNNGTC 1 cut(s) 744
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 1 cut(s) 516
Eam1105I GACNNNNNGTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 516
Eco130I CCWWGG 1 cut(s) 31
Eco147I AGGCCT 1 cut(s) 1160
Eco24I GRGCYC 1 cut(s) 553
Eco47I GGWCC 3 cut(s) 410, 721, 985
Eco57I CTGAAG 2 cut(s) 1092, 1239
Eco88I CYCGRG 2 cut(s) 554, 925
EcoO109I RGGNCCY 2 cut(s) 721, 985
EcoRI GAATTC 2 cut(s) 880, 901
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 1 cut(s) 553
EgeI GGCGCC 1 cut(s) 501
EheI GGCGCC 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 4 cut(s) 184, 191, 235, 328
FaqI GGGAC 4 cut(s) 94, 734, 998, 1235
FatI CATG 4 cut(s) 180, 187, 231, 324
FbaI TGATCA 2 cut(s) 916, 949
Fnu4HI GCNGC 9 cut(s) 19, 250, 274, 277, 456, 459, 481, 609, 656
FokI GGATG 3 cut(s) 444, 1016, 1076
FriOI GRGCYC 1 cut(s) 553
Fsp4HI GCNGC 9 cut(s) 19, 250, 274, 277, 456, 459, 481, 609, 656
FspBI CTAG 4 cut(s) 338, 438, 744, 810
GlaI GCGC 2 cut(s) 501, 594
GluI GCNGC 9 cut(s) 19, 250, 274, 277, 456, 459, 481, 609, 656
HaeII RGCGCY 2 cut(s) 503, 596
HaeIII GGCC 4 cut(s) 30, 285, 601, 1160
HapII CCGG 4 cut(s) 75, 246, 452, 582
HhaI GCGC 2 cut(s) 502, 595
Hin1I GRCGYC 1 cut(s) 500
Hin1II CATG 4 cut(s) 184, 191, 235, 328
Hin6I GCGC 2 cut(s) 500, 593
HinP1I GCGC 2 cut(s) 500, 593
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HindIII AAGCTT 2 cut(s) 670, 1264
HinfI GANTC 4 cut(s) 296, 664, 893, 1227
HpaII CCGG 4 cut(s) 75, 246, 452, 582
HphI GGTGA 2 cut(s) 340, 413
Hpy166II GTNNAC 4 cut(s) 164, 220, 292, 561
Hpy188I TCNGA 7 cut(s) 308, 682, 898, 946, 954, 962, 1114
Hpy188III TCNNGA 4 cut(s) 863, 869, 914, 1214
Hpy8I GTNNAC 4 cut(s) 164, 220, 292, 561
Hpy99I CGWCG 1 cut(s) 545
HpyAV CCTTC 2 cut(s) 1220, 1263
HpyCH4III ACNGT 4 cut(s) 49, 410, 625, 966
HpyCH4IV ACGT 2 cut(s) 240, 543
HpyCH4V TGCA 8 cut(s) 21, 127, 266, 349, 422, 641, 1119, 1164
HpyF10VI GCNNNNNNNGC 8 cut(s) 27, 75, 133, 282, 443, 452, 467, 757
HpyF3I CTNAG 6 cut(s) 55, 102, 660, 679, 1111, 1127
HpySE526I ACGT 2 cut(s) 240, 543
Hsp92I GRCGYC 1 cut(s) 500
Hsp92II CATG 4 cut(s) 184, 191, 235, 328
HspAI GCGC 2 cut(s) 500, 593
KasI GGCGCC 1 cut(s) 499
KflI GGGWCCC 2 cut(s) 721, 985
Ksp22I TGATCA 2 cut(s) 916, 949
KspI CCGCGG 1 cut(s) 280
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 2 cut(s) 133, 1200
Lsp1109I GCAGC 6 cut(s) 5, 285, 467, 470, 620, 642
LweI GCATC 3 cut(s) 253, 1057, 1065
MaeI CTAG 4 cut(s) 338, 438, 744, 810
MaeII ACGT 2 cut(s) 240, 543
MaeIII GTNAC 2 cut(s) 371, 619
MboII GAAGA 6 cut(s) 218, 416, 533, 791, 794, 797
MfeI CAATTG 1 cut(s) 474
MflI RGATCY 1 cut(s) 833
MhlI GDGCHC 2 cut(s) 246, 553
MlsI TGGCCA 1 cut(s) 30
MluNI TGGCCA 1 cut(s) 30
Mly113I GGCGCC 1 cut(s) 500
MlyI GAGTC 1 cut(s) 1221
MmeI TCCRAC 3 cut(s) 716, 875, 1178
Mox20I TGGCCA 1 cut(s) 30
MroXI GAANNNNTTC 1 cut(s) 143
MscI TGGCCA 1 cut(s) 30
MseI TTAA 4 cut(s) 797, 801, 1103, 1140
MslI CAYNNNNRTG 1 cut(s) 488
Msp20I TGGCCA 1 cut(s) 30
MspA1I CMGCKG 3 cut(s) 101, 279, 611
MspI CCGG 4 cut(s) 75, 246, 452, 582
MspR9I CCNGG 1 cut(s) 246
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 2 cut(s) 349, 760
MvnI CGCG 3 cut(s) 279, 516, 536
MwoI GCNNNNNNNGC 8 cut(s) 27, 75, 133, 282, 443, 452, 467, 757
NarI GGCGCC 1 cut(s) 500
NciI CCSGG 1 cut(s) 246
NlaIII CATG 4 cut(s) 184, 191, 235, 328
NlaIV GGNNCC 6 cut(s) 501, 722, 723, 835, 986, 987
NmuCI GTSAC 1 cut(s) 371
NspV TTCGAA 1 cut(s) 933
PacI TTAATTAA 1 cut(s) 801
PceI AGGCCT 1 cut(s) 1160
PciSI GCTCTTC 1 cut(s) 516
PcsI WCGNNNNNNNCGW 1 cut(s) 540
PctI GAATGC 2 cut(s) 349, 760
PdmI GAANNNNTTC 1 cut(s) 143
PfeI GAWTC 3 cut(s) 296, 664, 893
PkrI GCNGC 9 cut(s) 20, 251, 275, 278, 457, 460, 482, 610, 657
PleI GAGTC 1 cut(s) 1221
PluTI GGCGCC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 1221
Ppu21I YACGTR 1 cut(s) 241
PpuMI RGGWCCY 2 cut(s) 721, 985
PshAI GACNNNNGTC 1 cut(s) 967
PshBI ATTAAT 2 cut(s) 801, 1140
Psp5II RGGWCCY 2 cut(s) 721, 985
PspN4I GGNNCC 6 cut(s) 501, 722, 723, 835, 986, 987
PspPI GGNCC 3 cut(s) 410, 721, 985
PspPPI RGGWCCY 2 cut(s) 721, 985
PstNI CAGNNNCTG 1 cut(s) 623
PsuI RGATCY 1 cut(s) 833
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 5 cut(s) 221, 239, 366, 562, 817
RsaNI GTAC 5 cut(s) 220, 238, 365, 561, 816
RseI CAYNNNNRTG 1 cut(s) 488
SacII CCGCGG 1 cut(s) 280
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 4 cut(s) 797, 801, 1103, 1140
SatI GCNGC 9 cut(s) 19, 250, 274, 277, 456, 459, 481, 609, 656
Sau96I GGNCC 3 cut(s) 410, 721, 985
SchI GAGTC 1 cut(s) 1221
ScrFI CCNGG 1 cut(s) 246
SduI GDGCHC 2 cut(s) 246, 553
SfaNI GCATC 3 cut(s) 253, 1057, 1065
SfcI CTRYAG 1 cut(s) 1077
SfoI GGCGCC 1 cut(s) 501
Sfr303I CCGCGG 1 cut(s) 280
SfuI TTCGAA 1 cut(s) 933
SgrBI CCGCGG 1 cut(s) 280
SinI GGWCC 3 cut(s) 410, 721, 985
SmiI ATTTAAAT 1 cut(s) 1104
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 1 cut(s) 1214
SmoI CTYRAG 1 cut(s) 1214
SseBI AGGCCT 1 cut(s) 1160
SsiI CCGC 6 cut(s) 99, 249, 277, 279, 480, 516
SspDI GGCGCC 1 cut(s) 499
SspMI CTAG 4 cut(s) 338, 438, 744, 810
StuI AGGCCT 1 cut(s) 1160
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 1 cut(s) 31
SwaI ATTTAAAT 1 cut(s) 1104
TaaI ACNGT 4 cut(s) 49, 410, 625, 966
TaiI ACGT 2 cut(s) 243, 546
TaqI TCGA 6 cut(s) 261, 570, 864, 933, 1059, 1230
TatI WGTACW 3 cut(s) 219, 560, 815
TauI GCSGC 3 cut(s) 252, 279, 483
TfiI GAWTC 3 cut(s) 296, 664, 893
Tru1I TTAA 4 cut(s) 797, 801, 1103, 1140
Tru9I TTAA 4 cut(s) 797, 801, 1103, 1140
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 6 cut(s) 18, 273, 455, 458, 608, 655
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 3 cut(s) 753, 831, 1059
TspGWI ACGGA 2 cut(s) 454, 730
VpaK11BI GGWCC 3 cut(s) 410, 721, 985
VspI ATTAAT 2 cut(s) 801, 1140
XapI RAATTY 4 cut(s) 141, 880, 901, 1105
XcmI CCANNNNNNNNNTGG 1 cut(s) 732
XmnI GAANNNNTTC 1 cut(s) 143
XspI CTAG 4 cut(s) 338, 438, 744, 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.