Rw2G044580

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0012850
Physical Location & Seq
Forward (+)
71219686 .. 71220959
1274 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G044580.1

Sequence Viewer

Length: 1269 bp
ATGGACTGGACTAGAGGCCACACCGTTGGCCGTGGCTCCTCCGCCGCCGTCTCCATCGCCACCTCTTCTGGCTCCGGCTCCAAAGTCTTTGCAGTCAAGTCTGCCGAGCTCTCCCAATCCGATTTCTTGCAAAGGGAGCAAAAAATTCTCTCTTCTTTGAGCAGTCCTCATGTGGTAAGCTATTTAGGACATGACATTACTAGAGAGAACAACAAGCTCATGTACAATATCTTCATGGAGTACGTGGCCGGCGGCACGATCATCGATGCAATTCGCAACCGTGGAGGCCAGTGTGAAGAATCAGCAATCGGGTTTTACACGAGGCAAATTGCCAAGGGGCTAGAGTACTTGCATTCAGTTGGATTAGTACATTGTGACATAAAAGGAAGGAACATCTTGGTTGGAGAAGAGGGTCCGAAAATTGCTGACTTTGGATGCTCCAGGTGGGCTGATCCTGCGGCTGAGGCAGTGCCAATTGGCGGCACGCCGATGTTTATGTCACCGGAGGTGGCGCGTGGGGAGGAGCAGGGTTTTCCTTGTGATGTTTGGGCTCTTGGGTGCACAGTTATCGAAATGGCTACCGGAGGATCAGTGCCGTGGCCTAATGCAGCTGACCCAGTAACTCTTCTTTACCGGATTGCATACTCCGGTGAGTCGCCGGAGATTCCGAGTTTTCTTTCAAATCAAGCAAGGGATTTCTTGGCAAAGTGTTTCATGAGGGACCCAAAAGAGAGGTGGACGGTTACACAACTTCTAAAGCATCCGTTTCTTGAGGAAATGATGATCATCAATTCTGCTAGTAGTACAAAGCAAGTTCAAGAATCCACTACTTCTTCATTTTCTCCAACAAGTATTCTTGATCAAGGGATATGGAATTCATTACAAGAATCAGAAACTTTGGGCAACCTTGTGGATCACCCAAGTGTTGAAAATTCGTACCCCGACGATAGGATCAGACTGCTGTCCCTGTTTTCAGGGATGCTCAGGTGGAGTTCTGATGAGGACTATTGGATCACAGTTAGGGGGAATGATTGTGGGGAAAGCAACCCAATTGTGAATGATGGTGAAGCTCAAGTTGATGTGATTGATAGCAAAATTAGTTGTAGAGATATTAATTTGAATAATATAGGAGCCTGTAAAAGTAGGAAAATTAGTAGCAGTATTAATAGCAATAGCAGTAGTACTAGTAGTGTAGTAGCTTTGAGCAATCTCATTTTGGAGAGGCACAAAGGCAATCTGTTATTTCCTTCAATACCAATTATCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009789 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010119 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048831 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090702 GO:0097305 GO:0097306 GO:0099120 GO:0099568 GO:0140096 GO:1900055 GO:1900057 GO:1901419 GO:1901421 GO:1901564 GO:1901700 GO:1901701 GO:1902456 GO:1902457 GO:1905623 GO:1905957 GO:1905959 GO:2000024 GO:2000026 GO:2000038
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

422

Amino Acids

46.14

Weight (kDa)

5.16

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 257 4.2e-51 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 7 - 253 2.6e-34 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05100 AT2G32510
fragaria_vesca FvH4_6g42900
malus_domestica MD09G1111000.v1.1 MD17G1098800.v1.1
prunus_persica Prupe.3G215000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0158821
rosa_laevigata RLG00000021116
rosa_multiflora Rmu_co8475471.1_g000001
rosa_roxburghii Rroxscaffold_2G00090970
rosa_rugosa Rorug02G0472800
rosa_samantha Rh2AG538600 Rh2AG538700 Rh2BG551300 Rh2CG521900 Rh2CG522000 Rh2DG561100
rosa_wichuraiana Rw2G044580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 514
AciI CCGC 5 cut(s) 42, 45, 252, 458, 480
AclWI GGATC 5 cut(s) 446, 595, 921, 959, 1019
AcoI YGGCCR 2 cut(s) 28, 246
AcsI RAATTY 3 cut(s) 144, 874, 931
AfaI GTAC 7 cut(s) 224, 242, 347, 369, 805, 938, 1183
AfiI CCNNNNNNNGG 2 cut(s) 479, 948
AgsI TTSAA 5 cut(s) 681, 818, 929, 1120, 1251
AhlI ACTAGT 1 cut(s) 1184
AjnI CCWGG 1 cut(s) 440
AleI CACNNNNGTG 1 cut(s) 921
AluBI AGCT 6 cut(s) 109, 180, 217, 611, 1070, 1199
AluI AGCT 6 cut(s) 109, 180, 217, 611, 1070, 1199
Alw21I GWGCWC 2 cut(s) 111, 563
Alw26I GTCTC 1 cut(s) 55
Alw44I GTGCAC 1 cut(s) 559
AlwI GGATC 5 cut(s) 446, 595, 921, 959, 1019
AoxI GGCC 5 cut(s) 16, 28, 246, 286, 599
ApaLI GTGCAC 1 cut(s) 559
ApeKI GCWGC 1 cut(s) 608
ApoI RAATTY 3 cut(s) 144, 874, 931
AseI ATTAAT 2 cut(s) 1113, 1164
AspLEI GCGC 1 cut(s) 514
AspS9I GGNCC 2 cut(s) 413, 721
AsuHPI GGTGA 4 cut(s) 492, 662, 908, 1076
AvaII GGWCC 2 cut(s) 413, 721
BaeGI GKGCMC 1 cut(s) 563
BanII GRGCYC 2 cut(s) 111, 553
BarI GAAGNNNNNNTAC 2 cut(s) 215, 247
BauI CACGAG 1 cut(s) 319
Bbv12I GWGCWC 2 cut(s) 111, 563
BbvCI CCTCAGC 1 cut(s) 462
BbvI GCAGC 1 cut(s) 620
BccI CCATC 2 cut(s) 62, 1055
BceAI ACGGC 3 cut(s) 15, 32, 580
BcgI CGANNNNNNTGC 4 cut(s) 244, 278, 550, 584
BciT130I CCWGG 1 cut(s) 442
BclI TGATCA 2 cut(s) 783, 859
BcoDI GTCTC 1 cut(s) 55
BcuI ACTAGT 1 cut(s) 1184
BfaI CTAG 5 cut(s) 12, 201, 341, 798, 1185
BisI GCNGC 5 cut(s) 45, 253, 459, 481, 609
BlsI GCNGC 5 cut(s) 46, 254, 460, 482, 610
BmcAI AGTACT 2 cut(s) 347, 1183
Bme1390I CCNGG 1 cut(s) 442
Bme18I GGWCC 2 cut(s) 413, 721
BmgT120I GGNCC 2 cut(s) 413, 721
BmiI GGNNCC 7 cut(s) 37, 73, 79, 414, 722, 723, 1132
BmrFI CCNGG 1 cut(s) 442
BmrI ACTGGG 1 cut(s) 611
BmsI GCATC 4 cut(s) 256, 425, 769, 969
BmuI ACTGGG 1 cut(s) 611
BoxI GACNNNNGTC 1 cut(s) 961
BplI GAGNNNNNCTC 2 cut(s) 151, 183
BpmI CTGGAG 1 cut(s) 424
Bpu10I CCTNAGC 2 cut(s) 462, 983
BpuEI CTTGAG 2 cut(s) 791, 1056
Bsa29I ATCGAT 1 cut(s) 264
BsaAI YACGTR 1 cut(s) 244
BsaBI GATNNNNATC 1 cut(s) 785
BsaJI CCNNGG 4 cut(s) 31, 280, 333, 596
BsaWI WCCGGW 4 cut(s) 502, 581, 633, 647
BsaXI ACNNNNNCTCC 2 cut(s) 576, 606
Bsc4I CCNNNNNNNGG 2 cut(s) 479, 948
Bse118I RCCGGY 1 cut(s) 248
Bse1I ACTGG 3 cut(s) 11, 289, 617
Bse8I GATNNNNATC 1 cut(s) 785
BseBI CCWGG 1 cut(s) 442
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 4 cut(s) 31, 280, 333, 596
BseGI GGATG 3 cut(s) 440, 760, 984
BseJI GATNNNNATC 1 cut(s) 785
BseLI CCNNNNNNNGG 2 cut(s) 479, 948
BseMII CTCAG 2 cut(s) 453, 997
BseNI ACTGG 3 cut(s) 11, 289, 617
BseRI GAGGAG 2 cut(s) 28, 536
BseSI GKGCMC 1 cut(s) 563
BseXI GCAGC 1 cut(s) 620
Bsh1236I CGCG 1 cut(s) 514
BshFI GGCC 5 cut(s) 18, 30, 248, 288, 601
BshVI ATCGAT 1 cut(s) 264
BsiHKAI GWGCWC 2 cut(s) 111, 563
BsiSI CCGG 7 cut(s) 75, 249, 503, 582, 634, 648, 659
BslFI GGGAC 2 cut(s) 734, 949
BslI CCNNNNNNNGG 2 cut(s) 479, 948
BsmAI GTCTC 1 cut(s) 55
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 2 cut(s) 734, 949
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 5 cut(s) 18, 30, 248, 288, 601
Bsp1286I GDGCHC 3 cut(s) 111, 553, 563
Bsp1407I TGTACA 1 cut(s) 222
Bsp143I GATC 8 cut(s) 258, 451, 587, 783, 859, 913, 951, 1011
BspACI CCGC 5 cut(s) 42, 45, 252, 458, 480
BspANI GGCC 5 cut(s) 18, 30, 248, 288, 601
BspCNI CTCAG 2 cut(s) 454, 996
BspDI ATCGAT 1 cut(s) 264
BspFNI CGCG 1 cut(s) 514
BspHI TCATGA 1 cut(s) 714
BspLI GGNNCC 7 cut(s) 37, 73, 79, 414, 722, 723, 1132
BspPI GGATC 5 cut(s) 446, 595, 921, 959, 1019
BsrFI RCCGGY 1 cut(s) 248
BsrGI TGTACA 1 cut(s) 222
BsrI ACTGG 3 cut(s) 11, 289, 617
BssAI RCCGGY 1 cut(s) 248
BssECI CCNNGG 4 cut(s) 31, 280, 333, 596
BssMI GATC 8 cut(s) 258, 451, 587, 783, 859, 913, 951, 1011
BssSI CACGAG 1 cut(s) 319
BssT1I CCWWGG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 319
Bst2UI CCWGG 1 cut(s) 442
Bst4CI ACNGT 5 cut(s) 25, 281, 565, 742, 1018
Bst6I CTCTTC 4 cut(s) 70, 157, 402, 630
BstAUI TGTACA 1 cut(s) 222
BstBAI YACGTR 1 cut(s) 244
BstC8I GCNNGC 2 cut(s) 250, 485
BstDEI CTNAG 2 cut(s) 462, 983
BstDSI CCRYGG 3 cut(s) 31, 280, 596
BstF5I GGATG 3 cut(s) 440, 760, 984
BstFNI CGCG 1 cut(s) 514
BstHHI GCGC 1 cut(s) 514
BstKTI GATC 8 cut(s) 261, 454, 590, 786, 862, 916, 954, 1014
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 8 cut(s) 258, 451, 587, 783, 859, 913, 951, 1011
BstMWI GCNNNNNNNGC 3 cut(s) 136, 455, 464
BstNI CCWGG 1 cut(s) 442
BstPAI GACNNNNGTC 1 cut(s) 961
BstSCI CCNGG 1 cut(s) 440
BstSLI GKGCMC 1 cut(s) 563
BstUI CGCG 1 cut(s) 514
BstV1I GCAGC 1 cut(s) 620
BstXI CCANNNNNNTGG 1 cut(s) 26
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 5 cut(s) 18, 30, 248, 288, 601
BsuTUI ATCGAT 1 cut(s) 264
BtgI CCRYGG 3 cut(s) 31, 280, 596
BtgZI GCGATG 1 cut(s) 40
BtsCI GGATG 3 cut(s) 440, 760, 984
BtsI GCAGTG 1 cut(s) 474
BtsIMutI CAGTG 3 cut(s) 296, 474, 597
Cac8I GCNNGC 2 cut(s) 250, 485
CciI TCATGA 1 cut(s) 714
CfoI GCGC 1 cut(s) 514
Cfr10I RCCGGY 1 cut(s) 248
Cfr13I GGNCC 2 cut(s) 413, 721
ClaI ATCGAT 1 cut(s) 264
Csp6I GTAC 7 cut(s) 223, 241, 346, 368, 804, 937, 1182
CviAII CATG 5 cut(s) 170, 191, 220, 235, 715
CviQI GTAC 7 cut(s) 223, 241, 346, 368, 804, 937, 1182
DdeI CTNAG 2 cut(s) 462, 983
DpnI GATC 8 cut(s) 260, 453, 589, 785, 861, 915, 953, 1013
DpnII GATC 8 cut(s) 258, 451, 587, 783, 859, 913, 951, 1011
EaeI YGGCCR 2 cut(s) 28, 246
Eam1104I CTCTTC 4 cut(s) 70, 157, 402, 630
EarI CTCTTC 4 cut(s) 70, 157, 402, 630
EciI GGCGGA 1 cut(s) 31
Ecl136II GAGCTC 1 cut(s) 109
Eco130I CCWWGG 1 cut(s) 333
Eco24I GRGCYC 2 cut(s) 111, 553
Eco47I GGWCC 2 cut(s) 413, 721
Eco53kI GAGCTC 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 109
EcoO109I RGGNCCY 1 cut(s) 721
EcoRI GAATTC 1 cut(s) 874
EcoRII CCWGG 1 cut(s) 440
EcoT14I CCWWGG 1 cut(s) 333
EcoT38I GRGCYC 2 cut(s) 111, 553
ErhI CCWWGG 1 cut(s) 333
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 5 cut(s) 173, 194, 223, 238, 718
FaqI GGGAC 2 cut(s) 734, 949
FatI CATG 5 cut(s) 169, 190, 219, 234, 714
FbaI TGATCA 2 cut(s) 783, 859
Fnu4HI GCNGC 5 cut(s) 45, 253, 459, 481, 609
FokI GGATG 3 cut(s) 447, 747, 991
FriOI GRGCYC 2 cut(s) 111, 553
Fsp4HI GCNGC 5 cut(s) 45, 253, 459, 481, 609
FspBI CTAG 5 cut(s) 12, 201, 341, 798, 1185
GlaI GCGC 1 cut(s) 513
GluI GCNGC 5 cut(s) 45, 253, 459, 481, 609
GsuI CTGGAG 1 cut(s) 424
HaeIII GGCC 5 cut(s) 18, 30, 248, 288, 601
HapII CCGG 7 cut(s) 75, 249, 503, 582, 634, 648, 659
HhaI GCGC 1 cut(s) 514
Hin1II CATG 5 cut(s) 173, 194, 223, 238, 718
Hin6I GCGC 1 cut(s) 512
HinP1I GCGC 1 cut(s) 512
HinfI GANTC 5 cut(s) 299, 653, 664, 821, 887
HpaII CCGG 7 cut(s) 75, 249, 503, 582, 634, 648, 659
HphI GGTGA 4 cut(s) 492, 662, 908, 1076
Hpy166II GTNNAC 2 cut(s) 561, 738
Hpy188I TCNGA 6 cut(s) 121, 417, 669, 892, 956, 997
Hpy188III TCNNGA 4 cut(s) 715, 770, 818, 857
Hpy8I GTNNAC 2 cut(s) 561, 738
Hpy99I CGWCG 1 cut(s) 947
HpyAV CCTTC 2 cut(s) 381, 1257
HpyCH4III ACNGT 5 cut(s) 25, 281, 565, 742, 1018
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 7 cut(s) 92, 130, 269, 352, 561, 608, 641
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 455, 464
HpyF3I CTNAG 2 cut(s) 462, 983
HpySE526I ACGT 1 cut(s) 243
Hsp92II CATG 5 cut(s) 173, 194, 223, 238, 718
HspAI GCGC 1 cut(s) 512
KflI GGGWCCC 1 cut(s) 721
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Ksp22I TGATCA 2 cut(s) 783, 859
Kzo9I GATC 8 cut(s) 258, 451, 587, 783, 859, 913, 951, 1011
LmnI GCTCC 7 cut(s) 41, 77, 83, 136, 443, 523, 1130
Lsp1109I GCAGC 1 cut(s) 620
LweI GCATC 4 cut(s) 256, 425, 769, 969
MaeI CTAG 5 cut(s) 12, 201, 341, 798, 1185
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 4 cut(s) 374, 498, 619, 742
MalI GATC 8 cut(s) 260, 453, 589, 785, 861, 915, 953, 1013
MboI GATC 8 cut(s) 258, 451, 587, 783, 859, 913, 951, 1011
MboII GAAGA 7 cut(s) 57, 144, 223, 308, 419, 617, 825
MfeI CAATTG 2 cut(s) 474, 1050
MhlI GDGCHC 3 cut(s) 111, 553, 563
MlyI GAGTC 1 cut(s) 662
MmeI TCCRAC 3 cut(s) 340, 382, 869
MroNI GCCGGC 1 cut(s) 248
MseI TTAA 3 cut(s) 1113, 1164, 1267
MslI CAYNNNNRTG 2 cut(s) 488, 921
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 7 cut(s) 75, 249, 503, 582, 634, 648, 659
MspR9I CCNGG 1 cut(s) 442
MunI CAATTG 2 cut(s) 474, 1050
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 1 cut(s) 442
MvnI CGCG 1 cut(s) 514
MwoI GCNNNNNNNGC 3 cut(s) 136, 455, 464
NaeI GCCGGC 1 cut(s) 250
NdeII GATC 8 cut(s) 258, 451, 587, 783, 859, 913, 951, 1011
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 5 cut(s) 173, 194, 223, 238, 718
NlaIV GGNNCC 7 cut(s) 37, 73, 79, 414, 722, 723, 1132
NmeAIII GCCGAG 1 cut(s) 130
NmuCI GTSAC 2 cut(s) 374, 498
OliI CACNNNNGTG 1 cut(s) 921
PagI TCATGA 1 cut(s) 714
PctI GAATGC 1 cut(s) 352
PdiI GCCGGC 1 cut(s) 250
PfeI GAWTC 4 cut(s) 299, 664, 821, 887
PkrI GCNGC 5 cut(s) 46, 254, 460, 482, 610
PleI GAGTC 1 cut(s) 661
PpsI GAGTC 1 cut(s) 661
Ppu21I YACGTR 1 cut(s) 244
PpuMI RGGWCCY 1 cut(s) 721
PshAI GACNNNNGTC 1 cut(s) 961
PshBI ATTAAT 2 cut(s) 1113, 1164
Psp124BI GAGCTC 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 721
Psp6I CCWGG 1 cut(s) 440
PspGI CCWGG 1 cut(s) 440
PspN4I GGNNCC 7 cut(s) 37, 73, 79, 414, 722, 723, 1132
PspPI GGNCC 2 cut(s) 413, 721
PspPPI RGGWCCY 1 cut(s) 721
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 7 cut(s) 224, 242, 347, 369, 805, 938, 1183
RsaNI GTAC 7 cut(s) 223, 241, 346, 368, 804, 937, 1182
RseI CAYNNNNRTG 2 cut(s) 488, 921
SacI GAGCTC 1 cut(s) 111
SaqAI TTAA 3 cut(s) 1113, 1164, 1267
SatI GCNGC 5 cut(s) 45, 253, 459, 481, 609
Sau3AI GATC 8 cut(s) 258, 451, 587, 783, 859, 913, 951, 1011
Sau96I GGNCC 2 cut(s) 413, 721
ScaI AGTACT 2 cut(s) 347, 1183
SchI GAGTC 1 cut(s) 662
ScrFI CCNGG 1 cut(s) 442
SduI GDGCHC 3 cut(s) 111, 553, 563
SfaNI GCATC 4 cut(s) 256, 425, 769, 969
SinI GGWCC 2 cut(s) 413, 721
SmiMI CAYNNNNRTG 2 cut(s) 488, 921
SmlI CTYRAG 2 cut(s) 770, 1071
SmoI CTYRAG 2 cut(s) 770, 1071
SpeI ACTAGT 1 cut(s) 1184
SsiI CCGC 5 cut(s) 42, 45, 252, 458, 480
SspMI CTAG 5 cut(s) 12, 201, 341, 798, 1185
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 1 cut(s) 440
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 5 cut(s) 25, 281, 565, 742, 1018
TaiI ACGT 1 cut(s) 246
TaqI TCGA 2 cut(s) 264, 570
TatI WGTACW 5 cut(s) 222, 345, 367, 803, 1181
TauI GCSGC 4 cut(s) 47, 255, 461, 483
TfiI GAWTC 4 cut(s) 299, 664, 821, 887
Tru1I TTAA 3 cut(s) 1113, 1164, 1267
Tru9I TTAA 3 cut(s) 1113, 1164, 1267
TscAI CASTG 3 cut(s) 296, 474, 597
TseFI GTSAC 2 cut(s) 374, 498
TseI GCWGC 1 cut(s) 608
Tsp45I GTSAC 2 cut(s) 374, 498
TspDTI ATGAA 4 cut(s) 223, 703, 825, 867
TspGWI ACGGA 1 cut(s) 753
TspRI CASTG 3 cut(s) 296, 474, 597
VneI GTGCAC 1 cut(s) 559
VpaK11BI GGWCC 2 cut(s) 413, 721
VspI ATTAAT 2 cut(s) 1113, 1164
XapI RAATTY 3 cut(s) 144, 874, 931
XcmI CCANNNNNNNNNTGG 1 cut(s) 732
XspI CTAG 5 cut(s) 12, 201, 341, 798, 1185
ZrmI AGTACT 2 cut(s) 347, 1183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.