MD17G1098800.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0012850
Physical Location & Seq
Reverse (-)
8387130 .. 8388395
1266 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1098800.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGGACTGGACAAGAGGCTCCACCATTGGCCAAGGCTCATCAGCCGCCGTCTCCTTAGCCACCTTTCGCAGCTCCGGTGACAAGTTTGCCGTCAAGTCCGCCCAGGTGTCTCAATCCGAGTTCTTGCAGAGGGAGCAGAAAATTCACTCTGTTTTGAGCAGTCGACATGTTGTAAGCTACATGGGACATGACATTACTAGTGAGAACAACAAGCTCATGTACAATCTTCTCATGGAGTACGTGCCCGGCGGCACCATCATCGATGCAATTCGCAGCCGCGGAGGCCTAGTTGACGAATCGATGATCAGAAACTACACGAGGGGCATTGTGAAGGGGCTAGACTACTTGCATTCACTTGGGTTGGTACATTGTGACATAAAAGGCAGGAACATCTTGGCTGGTGAAGACGGTCCGAAAATTGCAGATTTTGGATGTGCTAGGTGGGCTCATCCGGCAGTAGCTTCTGCCACCACAATTGGCGGGACGCCAATGTTTATGGCGCCGGAGGTGGCACGCGGAGAAGAGCAGGGGTTCGCGTGCGACATATGGGCTCTCGGGTGTACAATTATCGAAATGGCTACCGGAGGATCAGCGCCGTGGCCTAAAGCAGCTCACCCAGTAACTGTTCTGTATCAAATTGCATACTCTAATGACTTGCCTCAGATTCCAAGCTTCCTCTCAGAACAAGCAAAGCACTTTTTGGAAAAGTGCTTACAGAGGGACCCAACGGAGCGTTGGACGGCTAGTCAGCTTCTAAAACATTCATATCTTGGGCAAGAAGAAGAAGAAGAAGTTTTAATTAGTTCTCCTAAAATTACAAAACAAGTTCATGAGTCCACTTCATGTTCTCCAACAAGCATTCTCGATCAAGATATATGGAATTCTTTTGGGGAATCAGAGACTTCGGGCAGTCTTGATCAAAACCCGAGTTTCGAAAACTCCTCCGACGATCAGAGGATCAGACGGTTGTCGCTGTTTTCAGGGGACCCCAGTTGGAGTTTTGATGATGAGAGTTGGATCACAGTTAGAGAGAACGATTGTGATGAAAGCAACAGCATTGAGGACGATGCTGAAGGTGTAGGTAGTGAGAAGGATTTGGATTTAGATTTCTTAGATGGCAATAATATTAGTTGTAGAATTAATGTGGATATTTTAGGGGCCTGCCAAAAAAGTAGGAGAAATAGTAGTAGTGTAGGTTTAAGCAATCCCATTCTTGAGAGGGACAGAGATAATTTGTTACTTCCTTCGATTTCAAGTTTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009789 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010119 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048831 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090702 GO:0097305 GO:0097306 GO:0099120 GO:0099568 GO:0140096 GO:1900055 GO:1900057 GO:1901419 GO:1901421 GO:1901564 GO:1901700 GO:1901701 GO:1902456 GO:1902457 GO:1905623 GO:1905957 GO:1905959 GO:2000024 GO:2000026 GO:2000038
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

422

Amino Acids

46.37

Weight (kDa)

4.8

Isoelectric Point (pI)

55.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 4 - 257 7e-55 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 253 3.3e-32 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05100 AT2G32510
fragaria_vesca FvH4_6g42900
malus_domestica MD09G1111000.v1.1 MD17G1098800.v1.1
prunus_persica Prupe.3G215000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0158821
rosa_laevigata RLG00000021116
rosa_multiflora Rmu_co8475471.1_g000001
rosa_roxburghii Rroxscaffold_2G00090970
rosa_rugosa Rorug02G0472800
rosa_samantha Rh2AG538600 Rh2AG538700 Rh2BG551300 Rh2CG521900 Rh2CG522000 Rh2DG561100
rosa_wichuraiana Rw2G044580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 251, 499
AccI GTMKAC 1 cut(s) 163
AccII CGCG 3 cut(s) 279, 516, 536
AciI CCGC 7 cut(s) 45, 99, 249, 277, 279, 480, 516
AclWI GGATC 3 cut(s) 595, 965, 1025
AcoI YGGCCR 1 cut(s) 28
AcsI RAATTY 2 cut(s) 141, 880
AcuI CTGAAG 1 cut(s) 1092
AcyI GRCGYC 2 cut(s) 485, 500
AfaI GTAC 4 cut(s) 221, 239, 366, 562
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 1 cut(s) 1254
AhdI GACNNNNNGTC 1 cut(s) 744
AhlI ACTAGT 1 cut(s) 197
AjnI CCWGG 1 cut(s) 102
AluBI AGCT 7 cut(s) 72, 177, 214, 461, 611, 672, 751
AluI AGCT 7 cut(s) 72, 177, 214, 461, 611, 672, 751
Alw26I GTCTC 3 cut(s) 55, 114, 893
AlwI GGATC 3 cut(s) 595, 965, 1025
AlwNI CAGNNNCTG 1 cut(s) 623
Ama87I CYCGRG 2 cut(s) 554, 925
AoxI GGCC 4 cut(s) 28, 283, 599, 1158
ApeKI GCWGC 3 cut(s) 69, 273, 608
ApoI RAATTY 2 cut(s) 141, 880
AseI ATTAAT 1 cut(s) 1140
AspLEI GCGC 2 cut(s) 502, 595
AspS9I GGNCC 4 cut(s) 410, 721, 985, 1158
AsuC2I CCSGG 1 cut(s) 246
AsuHPI GGTGA 3 cut(s) 89, 413, 605
AsuII TTCGAA 1 cut(s) 933
AvaI CYCGRG 2 cut(s) 554, 925
AvaII GGWCC 3 cut(s) 410, 721, 985
BaeGI GKGCMC 1 cut(s) 246
BalI TGGCCA 1 cut(s) 30
BanI GGYRCC 2 cut(s) 251, 499
BanII GRGCYC 2 cut(s) 448, 553
BauI CACGAG 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 411
BbvI GCAGC 3 cut(s) 81, 285, 620
BccI CCATC 2 cut(s) 263, 1109
BceAI ACGGC 4 cut(s) 32, 74, 580, 756
BcgI CGANNNNNNTGC 2 cut(s) 241, 275
BciT130I CCWGG 1 cut(s) 104
BclI TGATCA 2 cut(s) 303, 916
BcnI CCSGG 1 cut(s) 246
BcoDI GTCTC 3 cut(s) 55, 114, 893
BcuI ACTAGT 1 cut(s) 197
BfaI CTAG 5 cut(s) 198, 287, 338, 438, 744
BfoI RGCGCY 2 cut(s) 503, 596
BglI GCCNNNNNGGC 1 cut(s) 282
BisI GCNGC 6 cut(s) 45, 70, 250, 274, 277, 609
BlsI GCNGC 6 cut(s) 46, 71, 251, 275, 278, 610
Bme1390I CCNGG 2 cut(s) 104, 246
Bme18I GGWCC 3 cut(s) 410, 721, 985
BmeRI GACNNNNNGTC 1 cut(s) 744
BmeT110I CYCGRG 2 cut(s) 554, 925
BmgT120I GGNCC 4 cut(s) 410, 721, 985, 1158
BmiI GGNNCC 8 cut(s) 19, 253, 501, 722, 723, 986, 987, 1159
BmrFI CCNGG 2 cut(s) 104, 246
BmrI ACTGGG 2 cut(s) 611, 984
BmsI GCATC 2 cut(s) 253, 1057
BmuI ACTGGG 2 cut(s) 611, 984
BoxI GACNNNNGTC 1 cut(s) 967
BpiI GAAGAC 1 cut(s) 411
Bpu10I CCTNAGC 1 cut(s) 55
Bpu14I TTCGAA 1 cut(s) 933
BpuEI CTTGAG 1 cut(s) 1235
BpuMI CCSGG 1 cut(s) 246
Bsa29I ATCGAT 2 cut(s) 261, 299
BsaAI YACGTR 1 cut(s) 241
BsaHI GRCGYC 2 cut(s) 485, 500
BsaJI CCNNGG 4 cut(s) 31, 102, 277, 596
BsaWI WCCGGW 2 cut(s) 74, 581
BsaXI ACNNNNNCTCC 4 cut(s) 273, 303, 1168, 1198
Bse1I ACTGG 3 cut(s) 11, 617, 990
BseBI CCWGG 1 cut(s) 104
BseCI ATCGAT 2 cut(s) 261, 299
BseDI CCNNGG 4 cut(s) 31, 102, 277, 596
BseGI GGATG 2 cut(s) 437, 448
BseMII CTCAG 2 cut(s) 674, 693
BseNI ACTGG 3 cut(s) 11, 617, 990
BseRI GAGGAG 1 cut(s) 931
BseSI GKGCMC 1 cut(s) 246
BseXI GCAGC 3 cut(s) 81, 285, 620
Bsh1236I CGCG 3 cut(s) 279, 516, 536
BshFI GGCC 4 cut(s) 30, 285, 601, 1160
BshNI GGYRCC 2 cut(s) 251, 499
BshVI ATCGAT 2 cut(s) 261, 299
BsiHKCI CYCGRG 2 cut(s) 554, 925
BsiSI CCGG 5 cut(s) 75, 246, 452, 503, 582
BslFI GGGAC 5 cut(s) 198, 496, 734, 998, 1235
BsmAI GTCTC 3 cut(s) 55, 114, 893
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 5 cut(s) 198, 496, 734, 998, 1235
BsmI GAATGC 2 cut(s) 349, 858
BsnI GGCC 4 cut(s) 30, 285, 601, 1160
BsoBI CYCGRG 2 cut(s) 554, 925
Bsp119I TTCGAA 1 cut(s) 933
Bsp1286I GDGCHC 3 cut(s) 246, 448, 553
Bsp1407I TGTACA 2 cut(s) 219, 560
Bsp143I GATC 7 cut(s) 303, 587, 865, 916, 949, 957, 1017
BspACI CCGC 7 cut(s) 45, 99, 249, 277, 279, 480, 516
BspANI GGCC 4 cut(s) 30, 285, 601, 1160
BspCNI CTCAG 2 cut(s) 673, 692
BspDI ATCGAT 2 cut(s) 261, 299
BspFNI CGCG 3 cut(s) 279, 516, 536
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 8 cut(s) 19, 253, 501, 722, 723, 986, 987, 1159
BspPI GGATC 3 cut(s) 595, 965, 1025
BspQI GCTCTTC 1 cut(s) 516
BspT104I TTCGAA 1 cut(s) 933
BspT107I GGYRCC 2 cut(s) 251, 499
BsrGI TGTACA 2 cut(s) 219, 560
BsrI ACTGG 3 cut(s) 11, 617, 990
BssECI CCNNGG 4 cut(s) 31, 102, 277, 596
BssMI GATC 7 cut(s) 303, 587, 865, 916, 949, 957, 1017
BssNI GRCGYC 2 cut(s) 485, 500
BssSI CACGAG 1 cut(s) 316
BssT1I CCWWGG 1 cut(s) 31
Bst2BI CACGAG 1 cut(s) 316
Bst2UI CCWGG 1 cut(s) 104
Bst4CI ACNGT 4 cut(s) 410, 625, 966, 1024
Bst6I CTCTTC 1 cut(s) 516
BstACI GRCGYC 2 cut(s) 485, 500
BstAUI TGTACA 2 cut(s) 219, 560
BstBAI YACGTR 1 cut(s) 241
BstBI TTCGAA 1 cut(s) 933
BstC8I GCNNGC 3 cut(s) 514, 538, 1162
BstDEI CTNAG 4 cut(s) 55, 660, 679, 1111
BstDSI CCRYGG 2 cut(s) 277, 596
BstF5I GGATG 2 cut(s) 437, 448
BstFNI CGCG 3 cut(s) 279, 516, 536
BstH2I RGCGCY 2 cut(s) 503, 596
BstHHI GCGC 2 cut(s) 502, 595
BstKTI GATC 7 cut(s) 306, 590, 868, 919, 952, 960, 1020
BstMAI GTCTC 3 cut(s) 55, 114, 893
BstMBI GATC 7 cut(s) 303, 587, 865, 916, 949, 957, 1017
BstMWI GCNNNNNNNGC 4 cut(s) 133, 282, 443, 452
BstNI CCWGG 1 cut(s) 104
BstNSI RCATGY 1 cut(s) 170
BstPAI GACNNNNGTC 1 cut(s) 967
BstSCI CCNGG 2 cut(s) 102, 244
BstSLI GKGCMC 1 cut(s) 246
BstUI CGCG 3 cut(s) 279, 516, 536
BstV1I GCAGC 3 cut(s) 81, 285, 620
BstV2I GAAGAC 1 cut(s) 411
Bsu15I ATCGAT 2 cut(s) 261, 299
BsuRI GGCC 4 cut(s) 30, 285, 601, 1160
BsuTUI ATCGAT 2 cut(s) 261, 299
BtgI CCRYGG 2 cut(s) 277, 596
BtsCI GGATG 2 cut(s) 437, 448
Cac8I GCNNGC 3 cut(s) 514, 538, 1162
CaiI CAGNNNCTG 1 cut(s) 623
CciI TCATGA 1 cut(s) 829
CfoI GCGC 2 cut(s) 502, 595
Cfr13I GGNCC 4 cut(s) 410, 721, 985, 1158
Cfr42I CCGCGG 1 cut(s) 280
ClaI ATCGAT 2 cut(s) 261, 299
CpoI CGGWCCG 1 cut(s) 410
CseI GACGC 1 cut(s) 493
Csp6I GTAC 4 cut(s) 220, 238, 365, 561
CspCI CAANNNNNGTGG 2 cut(s) 457, 492
CspI CGGWCCG 1 cut(s) 410
CviAII CATG 7 cut(s) 167, 181, 188, 217, 232, 830, 843
CviQI GTAC 4 cut(s) 220, 238, 365, 561
DdeI CTNAG 4 cut(s) 55, 660, 679, 1111
DinI GGCGCC 1 cut(s) 501
DpnI GATC 7 cut(s) 305, 589, 867, 918, 951, 959, 1019
DpnII GATC 7 cut(s) 303, 587, 865, 916, 949, 957, 1017
DriI GACNNNNNGTC 1 cut(s) 744
EaeI YGGCCR 1 cut(s) 28
Eam1104I CTCTTC 1 cut(s) 516
Eam1105I GACNNNNNGTC 1 cut(s) 744
EarI CTCTTC 1 cut(s) 516
EciI GGCGGA 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 31
Eco147I AGGCCT 1 cut(s) 285
Eco24I GRGCYC 2 cut(s) 448, 553
Eco47I GGWCC 3 cut(s) 410, 721, 985
Eco57I CTGAAG 1 cut(s) 1092
Eco88I CYCGRG 2 cut(s) 554, 925
EcoO109I RGGNCCY 3 cut(s) 721, 985, 1158
EcoRI GAATTC 1 cut(s) 880
EcoRII CCWGG 1 cut(s) 102
EcoT14I CCWWGG 1 cut(s) 31
EcoT38I GRGCYC 2 cut(s) 448, 553
EgeI GGCGCC 1 cut(s) 501
EheI GGCGCC 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 55
FaeI CATG 7 cut(s) 170, 184, 191, 220, 235, 833, 846
FaqI GGGAC 5 cut(s) 198, 496, 734, 998, 1235
FatI CATG 7 cut(s) 166, 180, 187, 216, 231, 829, 842
FauI CCCGC 1 cut(s) 473
FauNDI CATATG 1 cut(s) 545
FbaI TGATCA 2 cut(s) 303, 916
FblI GTMKAC 1 cut(s) 163
Fnu4HI GCNGC 6 cut(s) 45, 70, 250, 274, 277, 609
FokI GGATG 2 cut(s) 435, 444
FriOI GRGCYC 2 cut(s) 448, 553
Fsp4HI GCNGC 6 cut(s) 45, 70, 250, 274, 277, 609
FspBI CTAG 5 cut(s) 198, 287, 338, 438, 744
GlaI GCGC 2 cut(s) 501, 594
GluI GCNGC 6 cut(s) 45, 70, 250, 274, 277, 609
HaeII RGCGCY 2 cut(s) 503, 596
HaeIII GGCC 4 cut(s) 30, 285, 601, 1160
HapII CCGG 5 cut(s) 75, 246, 452, 503, 582
HgaI GACGC 1 cut(s) 493
HhaI GCGC 2 cut(s) 502, 595
Hin1I GRCGYC 2 cut(s) 485, 500
Hin1II CATG 7 cut(s) 170, 184, 191, 220, 235, 833, 846
Hin6I GCGC 2 cut(s) 500, 593
HinP1I GCGC 2 cut(s) 500, 593
HincII GTYRAC 2 cut(s) 164, 292
HindII GTYRAC 2 cut(s) 164, 292
HindIII AAGCTT 1 cut(s) 670
HinfI GANTC 4 cut(s) 296, 664, 833, 893
HpaII CCGG 5 cut(s) 75, 246, 452, 503, 582
HphI GGTGA 3 cut(s) 89, 413, 605
Hpy166II GTNNAC 4 cut(s) 164, 292, 561, 837
Hpy188I TCNGA 9 cut(s) 118, 308, 414, 663, 682, 898, 946, 954, 962
Hpy188III TCNNGA 5 cut(s) 830, 863, 869, 914, 1214
Hpy8I GTNNAC 4 cut(s) 164, 292, 561, 837
Hpy99I CGWCG 1 cut(s) 950
HpyAV CCTTC 4 cut(s) 325, 1067, 1084, 1254
HpyCH4III ACNGT 4 cut(s) 410, 625, 966, 1024
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 5 cut(s) 127, 266, 349, 422, 641
HpyF10VI GCNNNNNNNGC 4 cut(s) 133, 282, 443, 452
HpyF3I CTNAG 4 cut(s) 55, 660, 679, 1111
HpySE526I ACGT 1 cut(s) 240
Hsp92I GRCGYC 2 cut(s) 485, 500
Hsp92II CATG 7 cut(s) 170, 184, 191, 220, 235, 833, 846
HspAI GCGC 2 cut(s) 500, 593
KasI GGCGCC 1 cut(s) 499
KflI GGGWCCC 2 cut(s) 721, 985
Ksp22I TGATCA 2 cut(s) 303, 916
KspI CCGCGG 1 cut(s) 280
Kzo9I GATC 7 cut(s) 303, 587, 865, 916, 949, 957, 1017
LguI GCTCTTC 1 cut(s) 516
LmnI GCTCC 4 cut(s) 23, 77, 133, 730
Lsp1109I GCAGC 3 cut(s) 81, 285, 620
LweI GCATC 2 cut(s) 253, 1057
MaeI CTAG 5 cut(s) 198, 287, 338, 438, 744
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 4 cut(s) 77, 371, 619, 1236
MalI GATC 7 cut(s) 305, 589, 867, 918, 951, 959, 1019
MboI GATC 7 cut(s) 303, 587, 865, 916, 949, 957, 1017
MboII GAAGA 7 cut(s) 218, 416, 533, 791, 794, 797, 800
MfeI CAATTG 1 cut(s) 474
MhlI GDGCHC 3 cut(s) 246, 448, 553
MlsI TGGCCA 1 cut(s) 30
MluNI TGGCCA 1 cut(s) 30
Mly113I GGCGCC 1 cut(s) 500
MlyI GAGTC 1 cut(s) 842
MmeI TCCRAC 5 cut(s) 716, 875, 969, 974, 995
Mox20I TGGCCA 1 cut(s) 30
MscI TGGCCA 1 cut(s) 30
MseI TTAA 3 cut(s) 797, 1140, 1199
Msp20I TGGCCA 1 cut(s) 30
MspA1I CMGCKG 1 cut(s) 279
MspI CCGG 5 cut(s) 75, 246, 452, 503, 582
MspR9I CCNGG 2 cut(s) 104, 246
MunI CAATTG 1 cut(s) 474
Mva1269I GAATGC 2 cut(s) 349, 858
MvaI CCWGG 1 cut(s) 104
MvnI CGCG 3 cut(s) 279, 516, 536
MwoI GCNNNNNNNGC 4 cut(s) 133, 282, 443, 452
NarI GGCGCC 1 cut(s) 500
NciI CCSGG 1 cut(s) 246
NdeI CATATG 1 cut(s) 545
NdeII GATC 7 cut(s) 303, 587, 865, 916, 949, 957, 1017
NlaIII CATG 7 cut(s) 170, 184, 191, 220, 235, 833, 846
NlaIV GGNNCC 8 cut(s) 19, 253, 501, 722, 723, 986, 987, 1159
NmuCI GTSAC 2 cut(s) 77, 371
NspI RCATGY 1 cut(s) 170
NspV TTCGAA 1 cut(s) 933
PagI TCATGA 1 cut(s) 829
PceI AGGCCT 1 cut(s) 285
PciI ACATGT 1 cut(s) 166
PciSI GCTCTTC 1 cut(s) 516
PctI GAATGC 2 cut(s) 349, 858
PfeI GAWTC 3 cut(s) 296, 664, 893
PkrI GCNGC 6 cut(s) 46, 71, 251, 275, 278, 610
PleI GAGTC 1 cut(s) 841
PluTI GGCGCC 1 cut(s) 503
PpsI GAGTC 1 cut(s) 841
Ppu21I YACGTR 1 cut(s) 241
PpuMI RGGWCCY 2 cut(s) 721, 985
PscI ACATGT 1 cut(s) 166
PshAI GACNNNNGTC 1 cut(s) 967
PshBI ATTAAT 1 cut(s) 1140
Psp5II RGGWCCY 2 cut(s) 721, 985
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PspN4I GGNNCC 8 cut(s) 19, 253, 501, 722, 723, 986, 987, 1159
PspPI GGNCC 4 cut(s) 410, 721, 985, 1158
PspPPI RGGWCCY 2 cut(s) 721, 985
PstNI CAGNNNCTG 1 cut(s) 623
RsaI GTAC 4 cut(s) 221, 239, 366, 562
RsaNI GTAC 4 cut(s) 220, 238, 365, 561
Rsr2I CGGWCCG 1 cut(s) 410
RsrII CGGWCCG 1 cut(s) 410
SacII CCGCGG 1 cut(s) 280
SalI GTCGAC 1 cut(s) 162
SapI GCTCTTC 1 cut(s) 516
SaqAI TTAA 3 cut(s) 797, 1140, 1199
SatI GCNGC 6 cut(s) 45, 70, 250, 274, 277, 609
Sau3AI GATC 7 cut(s) 303, 587, 865, 916, 949, 957, 1017
Sau96I GGNCC 4 cut(s) 410, 721, 985, 1158
SchI GAGTC 1 cut(s) 842
ScrFI CCNGG 2 cut(s) 104, 246
SduI GDGCHC 3 cut(s) 246, 448, 553
SfaNI GCATC 2 cut(s) 253, 1057
SfoI GGCGCC 1 cut(s) 501
Sfr303I CCGCGG 1 cut(s) 280
SfuI TTCGAA 1 cut(s) 933
SgrBI CCGCGG 1 cut(s) 280
SinI GGWCC 3 cut(s) 410, 721, 985
SmlI CTYRAG 1 cut(s) 1214
SmoI CTYRAG 1 cut(s) 1214
SpeI ACTAGT 1 cut(s) 197
SseBI AGGCCT 1 cut(s) 285
SsiI CCGC 7 cut(s) 45, 99, 249, 277, 279, 480, 516
SspDI GGCGCC 1 cut(s) 499
SspI AATATT 1 cut(s) 1126
SspMI CTAG 5 cut(s) 198, 287, 338, 438, 744
StuI AGGCCT 1 cut(s) 285
StyD4I CCNGG 2 cut(s) 102, 244
StyI CCWWGG 1 cut(s) 31
TaaI ACNGT 4 cut(s) 410, 625, 966, 1024
TaiI ACGT 1 cut(s) 243
TaqI TCGA 7 cut(s) 163, 261, 299, 570, 864, 933, 1247
TatI WGTACW 2 cut(s) 219, 560
TauI GCSGC 3 cut(s) 47, 252, 279
TfiI GAWTC 3 cut(s) 296, 664, 893
Tru1I TTAA 3 cut(s) 797, 1140, 1199
Tru9I TTAA 3 cut(s) 797, 1140, 1199
TseFI GTSAC 2 cut(s) 77, 371
TseI GCWGC 3 cut(s) 69, 273, 608
Tsp45I GTSAC 2 cut(s) 77, 371
TspDTI ATGAA 4 cut(s) 753, 818, 831, 1059
TspGWI ACGGA 1 cut(s) 743
VpaK11BI GGWCC 3 cut(s) 410, 721, 985
VspI ATTAAT 1 cut(s) 1140
XapI RAATTY 2 cut(s) 141, 880
XceI RCATGY 1 cut(s) 170
XcmI CCANNNNNNNNNTGG 1 cut(s) 732
XmiI GTMKAC 1 cut(s) 163
XspI CTAG 5 cut(s) 198, 287, 338, 438, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.