Rorug02G0472800

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0012850
Physical Location & Seq
Reverse (-)
60410314 .. 60411712
1399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0472800.1

Sequence Viewer

Length: 489 bp
ATGGGTGTTCTTGCAACGATTGCTAAGAATTTGGATACATTGATCGGGCCAGGAGTTACACTTCTTTATCCTTTATATGCAACAATGAGAGCAATAGAGAACCCTTCTGCACTAGACGATCAGCAGTGGCTTACATATTGGGTTTTATATTCTTTGATCACATTATTCGAGCTATTCTGTTGGAAAGTCCTCGCTTGGATTCCAATATGGCCATATTTGAAGCTGTTTTTTAGCATGTGGTTGGTGTTGCCAAAATTCAATGGAGCTGCCTACATCTACGAAAATATTGTGAGAAAATATGTGAAGCCCGGTGACTATATCAATTTTATCAGCTCATCATCATCAAAGCGCCCAGAGGGACAGAATAAGGTCCTCCAAATGATGAGCCTGGATGCAAGGAAATCAGTAGAGCGTTTTATCGATCAACACGGACCCGAGGCTTTCGAAAGAGTCGTCAAAGCAGCTGAAAGAGAAGCGAGGAAGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0001932 GO:0001934 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005938 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009628 GO:0009653 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009789 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010119 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0030587 GO:0031098 GO:0031323 GO:0031325 GO:0031399 GO:0031401 GO:0032147 GO:0032268 GO:0032270 GO:0032502 GO:0032870 GO:0033554 GO:0033674 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043549 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048580 GO:0048583 GO:0048584 GO:0048831 GO:0048856 GO:0050789 GO:0050790 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051171 GO:0051173 GO:0051174 GO:0051239 GO:0051240 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051703 GO:0051704 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:0090702 GO:0097305 GO:0097306 GO:0099120 GO:0099568 GO:0140096 GO:1900055 GO:1900057 GO:1901419 GO:1901421 GO:1901564 GO:1901700 GO:1901701 GO:1902456 GO:1902457 GO:1905623 GO:1905957 GO:1905959 GO:2000024 GO:2000026 GO:2000038
KEGG Pathways
Metabolic & Signaling
ko04016 map04016
Pfam Domains
Protein Families
Pkinase

Protein Analysis

162

Amino Acids

18.81

Weight (kDa)

9.14

Isoelectric Point (pI)

29.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TB2_DP1_HVA22 PF03134 24 - 100 2.3e-27 TB2/DP1, HVA22 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012850)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G05100 AT2G32510
fragaria_vesca FvH4_6g42900
malus_domestica MD09G1111000.v1.1 MD17G1098800.v1.1
prunus_persica Prupe.3G215000_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0158821
rosa_laevigata RLG00000021116
rosa_multiflora Rmu_co8475471.1_g000001
rosa_roxburghii Rroxscaffold_2G00090970
rosa_rugosa Rorug02G0472800
rosa_samantha Rh2AG538600 Rh2AG538700 Rh2BG551300 Rh2CG521900 Rh2CG522000 Rh2DG561100
rosa_wichuraiana Rw2G044580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 209
AcsI RAATTY 2 cut(s) 28, 254
AgsI TTSAA 2 cut(s) 220, 259
AjnI CCWGG 2 cut(s) 49, 387
AluBI AGCT 5 cut(s) 172, 223, 266, 333, 464
AluI AGCT 5 cut(s) 172, 223, 266, 333, 464
Ama87I CYCGRG 1 cut(s) 434
AoxI GGCC 2 cut(s) 47, 209
ApeKI GCWGC 2 cut(s) 266, 461
ApoI RAATTY 2 cut(s) 28, 254
ArsI GACNNNNNNTTYG 2 cut(s) 438, 470
AspLEI GCGC 1 cut(s) 351
AspS9I GGNCC 3 cut(s) 47, 370, 431
AsuC2I CCSGG 1 cut(s) 309
AsuHPI GGTGA 1 cut(s) 323
AsuII TTCGAA 1 cut(s) 444
AvaI CYCGRG 1 cut(s) 434
AvaII GGWCC 2 cut(s) 370, 431
BalI TGGCCA 1 cut(s) 211
BbvI GCAGC 2 cut(s) 253, 473
BciT130I CCWGG 2 cut(s) 51, 389
BciVI GTATCC 1 cut(s) 28
BclI TGATCA 1 cut(s) 156
BcnI CCSGG 1 cut(s) 309
BfaI CTAG 1 cut(s) 113
BfoI RGCGCY 1 cut(s) 352
BfuI GTATCC 1 cut(s) 28
BisI GCNGC 2 cut(s) 267, 462
BlsI GCNGC 2 cut(s) 268, 463
Bme1390I CCNGG 3 cut(s) 51, 309, 389
Bme18I GGWCC 2 cut(s) 370, 431
BmeT110I CYCGRG 1 cut(s) 434
BmgT120I GGNCC 3 cut(s) 47, 370, 431
BmiI GGNNCC 1 cut(s) 433
BmrFI CCNGG 3 cut(s) 51, 309, 389
BmsI GCATC 1 cut(s) 382
Bpu14I TTCGAA 1 cut(s) 444
BpuMI CCSGG 1 cut(s) 309
Bsa29I ATCGAT 1 cut(s) 420
BsaJI CCNNGG 1 cut(s) 435
BseBI CCWGG 2 cut(s) 51, 389
BseCI ATCGAT 1 cut(s) 420
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 1 cut(s) 397
BseXI GCAGC 2 cut(s) 253, 473
BsgI GTGCAG 1 cut(s) 93
BshFI GGCC 2 cut(s) 49, 211
BshVI ATCGAT 1 cut(s) 420
BsiHKCI CYCGRG 1 cut(s) 434
BsiSI CCGG 1 cut(s) 309
BslFI GGGAC 1 cut(s) 372
BsmFI GGGAC 1 cut(s) 372
BsnI GGCC 2 cut(s) 49, 211
BsoBI CYCGRG 1 cut(s) 434
Bsp119I TTCGAA 1 cut(s) 444
Bsp143I GATC 4 cut(s) 42, 118, 156, 421
BspANI GGCC 2 cut(s) 49, 211
BspDI ATCGAT 1 cut(s) 420
BspLI GGNNCC 1 cut(s) 433
BspT104I TTCGAA 1 cut(s) 444
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 4 cut(s) 42, 118, 156, 421
Bst2UI CCWGG 2 cut(s) 51, 389
BstAPI GCANNNNNTGC 1 cut(s) 20
BstBI TTCGAA 1 cut(s) 444
BstDEI CTNAG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 397
BstH2I RGCGCY 1 cut(s) 352
BstHHI GCGC 1 cut(s) 351
BstKTI GATC 4 cut(s) 45, 121, 159, 424
BstMBI GATC 4 cut(s) 42, 118, 156, 421
BstMWI GCNNNNNNNGC 1 cut(s) 20
BstNI CCWGG 2 cut(s) 51, 389
BstNSI RCATGY 1 cut(s) 238
BstSCI CCNGG 3 cut(s) 49, 307, 387
BstV1I GCAGC 2 cut(s) 253, 473
Bsu15I ATCGAT 1 cut(s) 420
BsuI GTATCC 1 cut(s) 28
BsuRI GGCC 2 cut(s) 49, 211
BsuTUI ATCGAT 1 cut(s) 420
BtsCI GGATG 1 cut(s) 397
BtsI GCAGTG 1 cut(s) 131
BtsIMutI CAGTG 2 cut(s) 131, 484
CfoI GCGC 1 cut(s) 351
Cfr13I GGNCC 3 cut(s) 47, 370, 431
ClaI ATCGAT 1 cut(s) 420
CviAII CATG 1 cut(s) 235
DdeI CTNAG 1 cut(s) 24
DpnI GATC 4 cut(s) 44, 120, 158, 423
DpnII GATC 4 cut(s) 42, 118, 156, 421
EaeI YGGCCR 1 cut(s) 209
Eco47I GGWCC 2 cut(s) 370, 431
Eco88I CYCGRG 1 cut(s) 434
EcoO109I RGGNCCY 1 cut(s) 370
EcoRII CCWGG 2 cut(s) 49, 387
FaeI CATG 1 cut(s) 238
FaiI YATR 9 cut(s) 76, 78, 136, 148, 208, 214, 236, 300, 318
FaqI GGGAC 1 cut(s) 372
FatI CATG 1 cut(s) 234
FbaI TGATCA 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 267, 462
FokI GGATG 1 cut(s) 404
Fsp4HI GCNGC 2 cut(s) 267, 462
FspBI CTAG 1 cut(s) 113
GlaI GCGC 1 cut(s) 350
GluI GCNGC 2 cut(s) 267, 462
HaeII RGCGCY 1 cut(s) 352
HaeIII GGCC 2 cut(s) 49, 211
HapII CCGG 1 cut(s) 309
HhaI GCGC 1 cut(s) 351
Hin1II CATG 1 cut(s) 238
Hin6I GCGC 1 cut(s) 349
HinP1I GCGC 1 cut(s) 349
HinfI GANTC 2 cut(s) 199, 450
HpaII CCGG 1 cut(s) 309
HphI GGTGA 1 cut(s) 323
HpyAV CCTTC 1 cut(s) 114
HpyCH4V TGCA 4 cut(s) 14, 80, 110, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 20
HpyF3I CTNAG 1 cut(s) 24
Hsp92II CATG 1 cut(s) 238
HspAI GCGC 1 cut(s) 349
Ksp22I TGATCA 1 cut(s) 156
Kzo9I GATC 4 cut(s) 42, 118, 156, 421
LmnI GCTCC 1 cut(s) 263
LpnPI CCDG 6 cut(s) 36, 63, 322, 366, 374, 401
Lsp1109I GCAGC 2 cut(s) 253, 473
LweI GCATC 1 cut(s) 382
MaeI CTAG 1 cut(s) 113
MaeIII GTNAC 2 cut(s) 55, 311
MalI GATC 4 cut(s) 44, 120, 158, 423
MboI GATC 4 cut(s) 42, 118, 156, 421
MlsI TGGCCA 1 cut(s) 211
MluCI AATT 3 cut(s) 28, 254, 322
MluNI TGGCCA 1 cut(s) 211
MlyI GAGTC 1 cut(s) 459
MmeI TCCRAC 1 cut(s) 161
MnlI CCTC 5 cut(s) 200, 349, 383, 430, 471
Mox20I TGGCCA 1 cut(s) 211
MscI TGGCCA 1 cut(s) 211
Msp20I TGGCCA 1 cut(s) 211
MspA1I CMGCKG 1 cut(s) 464
MspI CCGG 1 cut(s) 309
MspR9I CCNGG 3 cut(s) 51, 309, 389
MvaI CCWGG 2 cut(s) 51, 389
MwoI GCNNNNNNNGC 1 cut(s) 20
NciI CCSGG 1 cut(s) 309
NdeII GATC 4 cut(s) 42, 118, 156, 421
NlaIII CATG 1 cut(s) 238
NlaIV GGNNCC 1 cut(s) 433
NmuCI GTSAC 1 cut(s) 311
NspI RCATGY 1 cut(s) 238
NspV TTCGAA 1 cut(s) 444
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PfeI GAWTC 1 cut(s) 199
PkrI GCNGC 2 cut(s) 268, 463
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
PpuMI RGGWCCY 1 cut(s) 370
Psp5II RGGWCCY 1 cut(s) 370
Psp6I CCWGG 2 cut(s) 49, 387
PspGI CCWGG 2 cut(s) 49, 387
PspN4I GGNNCC 1 cut(s) 433
PspPI GGNCC 3 cut(s) 47, 370, 431
PspPPI RGGWCCY 1 cut(s) 370
PvuII CAGCTG 1 cut(s) 464
SatI GCNGC 2 cut(s) 267, 462
Sau3AI GATC 4 cut(s) 42, 118, 156, 421
Sau96I GGNCC 3 cut(s) 47, 370, 431
SchI GAGTC 1 cut(s) 459
ScrFI CCNGG 3 cut(s) 51, 309, 389
SetI ASST 6 cut(s) 174, 225, 268, 335, 372, 466
SfaNI GCATC 1 cut(s) 382
SfuI TTCGAA 1 cut(s) 444
SinI GGWCC 2 cut(s) 370, 431
Sse9I AATT 3 cut(s) 28, 254, 322
SspI AATATT 1 cut(s) 286
SspMI CTAG 1 cut(s) 113
StyD4I CCNGG 3 cut(s) 49, 307, 387
TaqI TCGA 3 cut(s) 168, 420, 444
TasI AATT 3 cut(s) 28, 254, 322
TfiI GAWTC 1 cut(s) 199
TscAI CASTG 1 cut(s) 131
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 2 cut(s) 266, 461
Tsp45I GTSAC 1 cut(s) 311
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 1 cut(s) 131
VpaK11BI GGWCC 2 cut(s) 370, 431
XapI RAATTY 2 cut(s) 28, 254
XceI RCATGY 1 cut(s) 238
XspI CTAG 1 cut(s) 113
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.