AT2G32630

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0017068
Physical Location & Seq
Forward (+)
13844780 .. 13846708
1929 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32630.1

Sequence Viewer

Length: 1875 bp
ATGAACATGTCTTCTTCTTCTCGCAAACTCTCGCGACTCTTCAACATTGTAACTCAGAATCAGAAATCTCCAGTTTTGCTTTCTTCTGACCAAGAAGCAGCCAGAAGAATCACGGCGTGCCTGGTGGAGAAATCCACCATCGGAAAACTTCAATCGAATCCATCACTCCTCTTCAATTTGAATTCAAATGTTACCCGTCTCGTTCTATCTGAGCCAACTCTTCCCACTCAATCCTGCATCGATTTCTTCAAGCTTCTTCGCGAATTCGAATCTAATCTCAAACCTGATCTCACGGCGGTAGTGACTTTATCTCACCGGTTATATTCTAACCGGAGGTTCAATGAGATGAGATCGCTTCTGAATTCAGTTGTTAACGACGGGTTTTACAAGCGTCCGGTTGAGGAGTTAGGATCAGCGATGGTGGATTGTGACATTTCTGAAGAAAAGTTTGAATTTTTCGAAAAGTTTTTCGATTTGGTATTTAGGGTGTATGTTGATAACGGAATGTTTGAAGAGGGTTTAAGGGTTTTTGATTACATGGTTAAAAAGGGATTGAGTATTGATGAAAGATCCTGCATTGTGTTTTTGGTTGCGGCGAAGAAGCGACGGAGAATTGATCTCTGTTTGGAAATTTTCCGGCGAATGGTTGATTCTGGAGTGAAGATAACTGTTTATTCATTGACGATTGTGGTTGAAGGATTATGTAGAAGAGGTGAAGTTGAGAAGAGTAAGAAGCTGATTAAGGAGTTTTCTGTTAAAGGGATTAAACCTGAAGCTTATACTTACAACACTATTATCAATGCTTATGTTAAACAGAGAGATTTCTCGGGTGTGGAAGGGGTTTTGAAGGTGATGAAGAAGGATGGTGTGGTGTATAACAAGGTTACGTATACGCTTTTGATGGAATTGAGTGTTAAGAATGGGAAAATGAGTGATGCGGAGAAGCTGTTCGATGAAATGCGTGAGAGAGGAATAGAATCTGATGTTCATGTGTATACTTCTTTGATAAGTTGGAATTGTAGAAAGGGAAATATGAAAAGAGCGTTTTTGTTGTTTGATGAATTGACAGAGAAGGGACTTTCGCCGAGTAGTTATACCTACGGGGCGCTTATTGACGGGGTGTGTAAGGTAGGGGAAATGGGTGCAGCTGAGATATTGATGAACGAGATGCAAAGTAAAGGAGTTAATATCACTCAGGTGGTGTTTAATACGTTGATTGATGGGTATTGCAGGAAAGGGATGGTTGATGAAGCATCAATGATTTATGATGTGATGGAGCAGAAAGGGTTTCAAGCTGATGTTTTCACTTGTAATACGATTGCTAGCTGTTTCAATAGATTGAAAAGGTATGATGAGGCAAAACAATGGCTGTTCAGGATGATGGAGGGTGGTGTGAAGCTTAGCACGGTTAGTTATACTAATTTGATCGATGTTTACTGCAAAGAAGGGAACGTTGAAGAGGCAAAGAGGCTATTTGTGGAGATGAGCAGCAAGGGAGTACAGCCCAACGCTATCACGTACAATGTGATGATCTACGCTTACTGCAAGCAAGGGAAAATAAAAGAAGCACGTAAACTAAGGGCCAACATGGAAGCAAATGGGATGGATCCGGATTCATATACCTACACGTCGCTCATACACGGGGAATGTATTGCTGATAATGTGGATGAAGCAATGAGGCTCTTTAGCGAGATGGGGTTGAAGGGTTTGGACCAGAATTCTGTTACGTACACGGTGATGATCTCGGGTTTGTCCAAAGCTGGAAAATCAGACGAAGCCTTTGGATTGTACGATGAGATGAAAAGGAAAGGGTATACAATAGATAATAAGGTTTATACTGCACTCATTGGAAGTATGCATTCACCTGAGACTTAA

Protein Analysis

624

Amino Acids

70.89

Weight (kDa)

8.33

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 173 - 309 1.3e-09 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 228 - 271 4.5e-08 PPR repeat family
PPR_3 PF13812 246 - 307 2.6e-09 Pentatricopeptide repeat domain
PPR_2 PF13041 257 - 301 1.9e-09 PPR repeat family
PPR_3 PF13812 282 - 326 1.2e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 293 - 341 5.7e-12 PPR repeat family
PPR PF01535 295 - 325 2.6e-07 PPR repeat
PPR_long PF17177 312 - 427 3.1e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 315 - 372 1.3e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 323 - 354 3e-06 PPR repeat
PPR_2 PF13041 328 - 376 2.8e-14 PPR repeat family
PPR_1 PF12854 358 - 391 3.1e-07 PPR repeat
PPR_1 PF12854 393 - 426 2.6e-10 PPR repeat
PPR_2 PF13041 399 - 444 2.7e-12 PPR repeat family
PPR PF01535 400 - 430 8.7e-09 PPR repeat
PPR_long PF17177 448 - 546 2.4e-09 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 460 - 515 6.2e-11 Pentatricopeptide repeat domain
PPR_1 PF12854 464 - 496 1.1e-09 PPR repeat
PPR_2 PF13041 468 - 516 9.6e-19 PPR repeat family
PPR PF01535 470 - 500 1.7e-09 PPR repeat
PPR_1 PF12854 498 - 531 5.8e-10 PPR repeat
TPR_24 PF23276 498 - 599 1.9e-06 Fungal tetratrico peptide repeats
PPR_2 PF13041 503 - 550 6.3e-16 PPR repeat family
PPR PF01535 505 - 534 1.2e-06 PPR repeat
PPR_3 PF13812 527 - 582 3.3e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 533 - 565 2.3e-10 PPR repeat
PPR PF01535 540 - 569 4.3e-06 PPR repeat
PPR_2 PF13041 543 - 586 2.1e-10 PPR repeat family
PPR_1 PF12854 568 - 601 3.1e-08 PPR repeat
PPR_2 PF13041 573 - 618 6.1e-13 PPR repeat family
PPR PF01535 575 - 604 2.2e-10 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0017068)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G32630
fragaria_vesca FvH4_6g16190
malus_domestica MD04G1094800.v1.1
prunus_persica Prupe.6G234000_v2.0.a1
pyrus_communis pycom04g08980
rosa_chinensis RchiOBHm_Chr3g0470041
rosa_laevigata RLG00000024301
rosa_multiflora Rmu_co8368425.1_g000001 Rmu_sc0017440.1_g000003
rosa_roxburghii Rroxscaffold_6G00411260
rosa_rugosa Rorug03G0106300.1
rosa_samantha Rh3CG170300
rosa_wichuraiana Rw3G014650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 890, 995, 1814
AccII CGCG 2 cut(s) 34, 261
AccIII TCCGGA 1 cut(s) 1609
AciI CCGC 3 cut(s) 296, 593, 938
AclI AACGTT 1 cut(s) 1452
AclWI GGATC 4 cut(s) 418, 564, 1601, 1614
AcsI RAATTY 6 cut(s) 181, 263, 361, 452, 630, 1717
AcuI CTGAAG 2 cut(s) 459, 792
AdeI CACNNNGTG 1 cut(s) 117
AfaI GTAC 4 cut(s) 1500, 1520, 1730, 1790
AfiI CCNNNNNNNGG 1 cut(s) 643
AflIII ACRYGT 2 cut(s) 6, 1626
AgeI ACCGGT 1 cut(s) 315
AjiI CACGTC 1 cut(s) 1629
AjnI CCWGG 1 cut(s) 120
AleI CACNNNNGTG 1 cut(s) 1196
AloI GAACNNNNNNTCC 2 cut(s) 932, 964
AluBI AGCT 9 cut(s) 253, 736, 776, 946, 1148, 1295, 1326, 1399, 1760
AluI AGCT 9 cut(s) 253, 736, 776, 946, 1148, 1295, 1326, 1399, 1760
Alw26I GTCTC 2 cut(s) 203, 1862
AlwI GGATC 4 cut(s) 418, 564, 1601, 1614
Ama87I CYCGRG 2 cut(s) 826, 1744
Aor13HI TCCGGA 1 cut(s) 1609
AoxI GGCC 1 cut(s) 1581
ApeKI GCWGC 3 cut(s) 98, 1145, 1488
ApoI RAATTY 6 cut(s) 181, 263, 361, 452, 630, 1717
ArsI GACNNNNNNTTYG 2 cut(s) 1763, 1795
AsiGI ACCGGT 1 cut(s) 315
Asp700I GAANNNNTTC 1 cut(s) 947
AspLEI GCGC 1 cut(s) 1108
AspS9I GGNCC 2 cut(s) 1581, 1711
AsuHPI GGTGA 5 cut(s) 305, 725, 862, 1747, 1854
AsuII TTCGAA 2 cut(s) 267, 459
AsuNHI GCTAGC 1 cut(s) 1322
AvaI CYCGRG 2 cut(s) 826, 1744
AvaII GGWCC 1 cut(s) 1711
BamHI GGATCC 1 cut(s) 1606
BbsI GAAGAC 1 cut(s) 3
BbvI GCAGC 3 cut(s) 110, 1157, 1500
BceAI ACGGC 2 cut(s) 129, 309
BciT130I CCWGG 1 cut(s) 122
BcoDI GTCTC 2 cut(s) 203, 1862
BfaI CTAG 1 cut(s) 1323
BfoI RGCGCY 1 cut(s) 1109
BisI GCNGC 4 cut(s) 99, 594, 1146, 1489
BlpI GCTNAGC 1 cut(s) 1400
BlsI GCNGC 4 cut(s) 100, 595, 1147, 1490
Bme1390I CCNGG 1 cut(s) 122
Bme18I GGWCC 1 cut(s) 1711
BmeT110I CYCGRG 2 cut(s) 826, 1744
BmgBI CACGTC 1 cut(s) 1629
BmgT120I GGNCC 2 cut(s) 1581, 1711
BmiI GGNNCC 1 cut(s) 1608
BmrFI CCNGG 1 cut(s) 122
BmsI GCATC 4 cut(s) 246, 925, 1158, 1262
BmtI GCTAGC 1 cut(s) 1326
BpiI GAAGAC 1 cut(s) 3
BpmI CTGGAG 2 cut(s) 54, 675
Bpu1102I GCTNAGC 1 cut(s) 1400
Bpu14I TTCGAA 2 cut(s) 267, 459
Bsa29I ATCGAT 2 cut(s) 240, 1428
BsaAI YACGTR 4 cut(s) 888, 1518, 1571, 1728
BsaWI WCCGGW 4 cut(s) 315, 330, 394, 1609
BsaXI ACNNNNNCTCC 2 cut(s) 932, 962
Bsc4I CCNNNNNNNGG 1 cut(s) 643
Bse118I RCCGGY 1 cut(s) 315
Bse1I ACTGG 1 cut(s) 71
Bse3DI GCAATG 1 cut(s) 1680
BseAI TCCGGA 1 cut(s) 1609
BseBI CCWGG 1 cut(s) 122
BseCI ATCGAT 2 cut(s) 240, 1428
BseGI GGATG 5 cut(s) 868, 1245, 1383, 1608, 1672
BseLI CCNNNNNNNGG 1 cut(s) 643
BseMI GCAATG 1 cut(s) 1680
BseMII CTCAG 5 cut(s) 68, 201, 1140, 1208, 1857
BseNI ACTGG 1 cut(s) 71
BseRI GAGGAG 2 cut(s) 158, 416
BseXI GCAGC 3 cut(s) 110, 1157, 1500
BsgI GTGCAG 2 cut(s) 1164, 1824
Bsh1236I CGCG 2 cut(s) 34, 261
BshFI GGCC 1 cut(s) 1583
BshTI ACCGGT 1 cut(s) 315
BshVI ATCGAT 2 cut(s) 240, 1428
BsiHKCI CYCGRG 2 cut(s) 826, 1744
BsiSI CCGG 5 cut(s) 316, 331, 395, 637, 1610
BslFI GGGAC 1 cut(s) 1089
BslI CCNNNNNNNGG 1 cut(s) 643
BsmAI GTCTC 2 cut(s) 203, 1862
BsmBI CGTCTC 1 cut(s) 203
BsmFI GGGAC 1 cut(s) 1089
BsmI GAATGC 1 cut(s) 1858
BsnI GGCC 1 cut(s) 1583
BsoBI CYCGRG 2 cut(s) 826, 1744
Bsp119I TTCGAA 2 cut(s) 267, 459
Bsp13I TCCGGA 1 cut(s) 1609
Bsp143I GATC 9 cut(s) 286, 350, 410, 569, 616, 1425, 1530, 1606, 1740
Bsp1720I GCTNAGC 1 cut(s) 1400
Bsp68I TCGCGA 2 cut(s) 34, 261
BspACI CCGC 3 cut(s) 296, 593, 938
BspANI GGCC 1 cut(s) 1583
BspCNI CTCAG 5 cut(s) 67, 202, 1141, 1207, 1858
BspDI ATCGAT 2 cut(s) 240, 1428
BspEI TCCGGA 1 cut(s) 1609
BspFNI CGCG 2 cut(s) 34, 261
BspLI GGNNCC 1 cut(s) 1608
BspOI GCTAGC 1 cut(s) 1326
BspPI GGATC 4 cut(s) 418, 564, 1601, 1614
BspT104I TTCGAA 2 cut(s) 267, 459
BsrDI GCAATG 1 cut(s) 1680
BsrFI RCCGGY 1 cut(s) 315
BsrI ACTGG 1 cut(s) 71
BssAI RCCGGY 1 cut(s) 315
BssMI GATC 9 cut(s) 286, 350, 410, 569, 616, 1425, 1530, 1606, 1740
BssNAI GTATAC 3 cut(s) 891, 996, 1815
Bst1107I GTATAC 3 cut(s) 891, 996, 1815
Bst2UI CCWGG 1 cut(s) 122
Bst4CI ACNGT 3 cut(s) 670, 1408, 1735
Bst6I CTCTTC 7 cut(s) 44, 176, 225, 507, 703, 719, 1452
BstBAI YACGTR 4 cut(s) 888, 1518, 1571, 1728
BstBI TTCGAA 2 cut(s) 267, 459
BstC8I GCNNGC 3 cut(s) 118, 1324, 1547
BstDEI CTNAG 7 cut(s) 54, 210, 1149, 1194, 1400, 1577, 1866
BstF5I GGATG 5 cut(s) 868, 1245, 1383, 1608, 1672
BstFNI CGCG 2 cut(s) 34, 261
BstH2I RGCGCY 1 cut(s) 1109
BstHHI GCGC 1 cut(s) 1108
BstKTI GATC 9 cut(s) 289, 353, 413, 572, 619, 1428, 1533, 1609, 1743
BstMAI GTCTC 2 cut(s) 203, 1862
BstMBI GATC 9 cut(s) 286, 350, 410, 569, 616, 1425, 1530, 1606, 1740
BstNI CCWGG 1 cut(s) 122
BstNSI RCATGY 1 cut(s) 10
BstSCI CCNGG 1 cut(s) 120
BstSNI TACGTA 2 cut(s) 888, 1728
BstUI CGCG 2 cut(s) 34, 261
BstV1I GCAGC 3 cut(s) 110, 1157, 1500
BstV2I GAAGAC 1 cut(s) 3
BstX2I RGATCY 2 cut(s) 569, 1606
BstYI RGATCY 2 cut(s) 569, 1606
BstZ17I GTATAC 3 cut(s) 891, 996, 1815
Bsu15I ATCGAT 2 cut(s) 240, 1428
BsuRI GGCC 1 cut(s) 1583
BsuTUI ATCGAT 2 cut(s) 240, 1428
BtgZI GCGATG 1 cut(s) 431
BtrI CACGTC 1 cut(s) 1629
BtsCI GGATG 5 cut(s) 868, 1245, 1383, 1608, 1672
BtuMI TCGCGA 2 cut(s) 34, 261
Cac8I GCNNGC 3 cut(s) 118, 1324, 1547
CfoI GCGC 1 cut(s) 1108
Cfr10I RCCGGY 1 cut(s) 315
Cfr13I GGNCC 2 cut(s) 1581, 1711
ClaI ATCGAT 2 cut(s) 240, 1428
CseI GACGC 1 cut(s) 380
Csp6I GTAC 4 cut(s) 1499, 1519, 1729, 1789
CspAI ACCGGT 1 cut(s) 315
CviAII CATG 4 cut(s) 7, 538, 989, 1588
CviQI GTAC 4 cut(s) 1499, 1519, 1729, 1789
DdeI CTNAG 7 cut(s) 54, 210, 1149, 1194, 1400, 1577, 1866
DpnI GATC 9 cut(s) 288, 352, 412, 571, 618, 1427, 1532, 1608, 1742
DpnII GATC 9 cut(s) 286, 350, 410, 569, 616, 1425, 1530, 1606, 1740
DraIII CACNNNGTG 1 cut(s) 117
Eam1104I CTCTTC 7 cut(s) 44, 176, 225, 507, 703, 719, 1452
EarI CTCTTC 7 cut(s) 44, 176, 225, 507, 703, 719, 1452
Eco105I TACGTA 2 cut(s) 888, 1728
Eco47I GGWCC 1 cut(s) 1711
Eco57I CTGAAG 2 cut(s) 459, 792
Eco88I CYCGRG 2 cut(s) 826, 1744
EcoRI GAATTC 4 cut(s) 181, 263, 361, 1717
EcoRII CCWGG 1 cut(s) 120
EcoT22I ATGCAT 1 cut(s) 1860
Esp3I CGTCTC 1 cut(s) 203
FaeI CATG 4 cut(s) 10, 541, 992, 1591
FaqI GGGAC 1 cut(s) 1089
FatI CATG 4 cut(s) 6, 537, 988, 1587
FblI GTMKAC 3 cut(s) 890, 995, 1814
Fnu4HI GCNGC 4 cut(s) 99, 594, 1146, 1489
FokI GGATG 5 cut(s) 875, 1252, 1390, 1615, 1679
Fsp4HI GCNGC 4 cut(s) 99, 594, 1146, 1489
FspBI CTAG 1 cut(s) 1323
GlaI GCGC 1 cut(s) 1107
GluI GCNGC 4 cut(s) 99, 594, 1146, 1489
GsuI CTGGAG 2 cut(s) 54, 675
HaeII RGCGCY 1 cut(s) 1109
HaeIII GGCC 1 cut(s) 1583
HapII CCGG 5 cut(s) 316, 331, 395, 637, 1610
HgaI GACGC 1 cut(s) 380
HhaI GCGC 1 cut(s) 1108
Hin1II CATG 4 cut(s) 10, 541, 992, 1591
Hin6I GCGC 1 cut(s) 1106
HinP1I GCGC 1 cut(s) 1106
HincII GTYRAC 1 cut(s) 373
HindII GTYRAC 1 cut(s) 373
HindIII AAGCTT 3 cut(s) 251, 774, 1397
HinfI GANTC 8 cut(s) 36, 58, 108, 157, 269, 650, 977, 1613
HpaI GTTAAC 1 cut(s) 373
HpaII CCGG 5 cut(s) 316, 331, 395, 637, 1610
HphI GGTGA 5 cut(s) 305, 725, 862, 1747, 1854
Hpy166II GTNNAC 7 cut(s) 373, 891, 996, 1435, 1574, 1731, 1815
Hpy188I TCNGA 9 cut(s) 57, 63, 88, 143, 211, 360, 439, 982, 1771
Hpy188III TCNNGA 5 cut(s) 33, 260, 654, 1375, 1610
Hpy8I GTNNAC 7 cut(s) 373, 891, 996, 1435, 1574, 1731, 1815
Hpy99I CGWCG 3 cut(s) 380, 609, 1633
HpyAV CCTTC 7 cut(s) 689, 830, 841, 853, 1066, 1439, 1696
HpyCH4III ACNGT 3 cut(s) 670, 1408, 1735
HpyCH4IV ACGT 7 cut(s) 887, 1211, 1452, 1517, 1570, 1628, 1727
HpyCH4V TGCA 9 cut(s) 237, 576, 1145, 1171, 1230, 1440, 1545, 1841, 1858
HpyF3I CTNAG 7 cut(s) 54, 210, 1149, 1194, 1400, 1577, 1866
HpySE526I ACGT 7 cut(s) 887, 1211, 1452, 1517, 1570, 1628, 1727
Hsp92II CATG 4 cut(s) 10, 541, 992, 1591
HspAI GCGC 1 cut(s) 1106
Kpn2I TCCGGA 1 cut(s) 1609
KspAI GTTAAC 1 cut(s) 373
Kzo9I GATC 9 cut(s) 286, 350, 410, 569, 616, 1425, 1530, 1606, 1740
LmnI GCTCC 1 cut(s) 1276
Lsp1109I GCAGC 3 cut(s) 110, 1157, 1500
LweI GCATC 4 cut(s) 246, 925, 1158, 1262
MaeI CTAG 1 cut(s) 1323
MaeII ACGT 7 cut(s) 887, 1211, 1452, 1517, 1570, 1628, 1727
MaeIII GTNAC 6 cut(s) 49, 190, 301, 428, 883, 1723
MalI GATC 9 cut(s) 288, 352, 412, 571, 618, 1427, 1532, 1608, 1742
MboI GATC 9 cut(s) 286, 350, 410, 569, 616, 1425, 1530, 1606, 1740
MflI RGATCY 2 cut(s) 569, 1606
MlyI GAGTC 1 cut(s) 30
MmeI TCCRAC 1 cut(s) 992
Mph1103I ATGCAT 1 cut(s) 1860
MroI TCCGGA 1 cut(s) 1609
MroXI GAANNNNTTC 1 cut(s) 947
MslI CAYNNNNRTG 2 cut(s) 1196, 1736
MspA1I CMGCKG 1 cut(s) 1148
MspI CCGG 5 cut(s) 316, 331, 395, 637, 1610
MspR9I CCNGG 1 cut(s) 122
Mva1269I GAATGC 1 cut(s) 1858
MvaI CCWGG 1 cut(s) 122
MvnI CGCG 2 cut(s) 34, 261
NdeII GATC 9 cut(s) 286, 350, 410, 569, 616, 1425, 1530, 1606, 1740
NheI GCTAGC 1 cut(s) 1322
NlaIII CATG 4 cut(s) 10, 541, 992, 1591
NlaIV GGNNCC 1 cut(s) 1608
NmeAIII GCCGAG 1 cut(s) 1110
NmuCI GTSAC 2 cut(s) 301, 428
NruI TCGCGA 2 cut(s) 34, 261
NsiI ATGCAT 1 cut(s) 1860
NspI RCATGY 1 cut(s) 10
NspV TTCGAA 2 cut(s) 267, 459
OliI CACNNNNGTG 1 cut(s) 1196
PciI ACATGT 1 cut(s) 6
PctI GAATGC 1 cut(s) 1858
PdmI GAANNNNTTC 1 cut(s) 947
PfeI GAWTC 7 cut(s) 58, 108, 157, 269, 650, 977, 1613
PinAI ACCGGT 1 cut(s) 315
PkrI GCNGC 4 cut(s) 100, 595, 1147, 1490
PleI GAGTC 1 cut(s) 30
PpsI GAGTC 1 cut(s) 30
Ppu21I YACGTR 4 cut(s) 888, 1518, 1571, 1728
PscI ACATGT 1 cut(s) 6
Psp1406I AACGTT 1 cut(s) 1452
Psp6I CCWGG 1 cut(s) 120
PspGI CCWGG 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 1608
PspPI GGNCC 2 cut(s) 1581, 1711
PsuI RGATCY 2 cut(s) 569, 1606
PvuII CAGCTG 1 cut(s) 1148
RruI TCGCGA 2 cut(s) 34, 261
RsaI GTAC 4 cut(s) 1500, 1520, 1730, 1790
RsaNI GTAC 4 cut(s) 1499, 1519, 1729, 1789
RseI CAYNNNNRTG 2 cut(s) 1196, 1736
SatI GCNGC 4 cut(s) 99, 594, 1146, 1489
Sau3AI GATC 9 cut(s) 286, 350, 410, 569, 616, 1425, 1530, 1606, 1740
Sau96I GGNCC 2 cut(s) 1581, 1711
SchI GAGTC 1 cut(s) 30
ScrFI CCNGG 1 cut(s) 122
SfaNI GCATC 4 cut(s) 246, 925, 1158, 1262
SfuI TTCGAA 2 cut(s) 267, 459
SinI GGWCC 1 cut(s) 1711
SmiMI CAYNNNNRTG 2 cut(s) 1196, 1736
SnaBI TACGTA 2 cut(s) 888, 1728
SsiI CCGC 3 cut(s) 296, 593, 938
SspMI CTAG 1 cut(s) 1323
StyD4I CCNGG 1 cut(s) 120
TaaI ACNGT 3 cut(s) 670, 1408, 1735
TaiI ACGT 7 cut(s) 890, 1214, 1455, 1520, 1573, 1631, 1730
TaqI TCGA 7 cut(s) 155, 240, 267, 459, 471, 951, 1428
TatI WGTACW 1 cut(s) 1498
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 7 cut(s) 58, 108, 157, 269, 650, 977, 1613
TseFI GTSAC 2 cut(s) 301, 428
TseI GCWGC 3 cut(s) 98, 1145, 1488
Tsp45I GTSAC 2 cut(s) 301, 428
TspGWI ACGGA 2 cut(s) 516, 622
VpaK11BI GGWCC 1 cut(s) 1711
XapI RAATTY 6 cut(s) 181, 263, 361, 452, 630, 1717
XceI RCATGY 1 cut(s) 10
XmiI GTMKAC 3 cut(s) 890, 995, 1814
XmnI GAANNNNTTC 1 cut(s) 947
XspI CTAG 1 cut(s) 1323
Zsp2I ATGCAT 1 cut(s) 1860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.