AT2G33560

Mad3/BUB1 hoMad3/BUB1 homology region 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0014113
Physical Location & Seq
Reverse (-)
14213601 .. 14216054
2454 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33560.2

Sequence Viewer

Length: 1188 bp
ATGGCAGCCGAAACGAAGGTTCAGGTTTCAGATCCAGAGGCAGAGTTTCTTAATTCGAAGCAGGAGACTGGATACGAGTGGGAGCTTTTCAAGGAGAACGTCCGGCCATTGAAGAGAGGTCGCAATGTTGGTATTCTCAACCACGCTCTCAAATCTCACTCTGACCACCAATTGAGAAAGAATCTCATCGAGAAACGCAGGAATTTGATTGAAGCCATTGATGAGTATGAAGGAGATGACCCTTTATCTCCATGGATAGAGTGTATAAAATGGGTACAAGAGGCTTTTCCACCAGGAGGAGAATGTTCAGGACTGTTAGTGATATATGAGCAATGTGTTCGTAAATTTTGGCACTCTGAACGTTACAAGGATGATCTTCGTTATCTTAAAGTCTGGTTGGAATATGCGGAGCATTGCGCTGATGCGGAAGTGATTTACAAGTTTTTGGAGGTCAATGAGATTGGAAAGACACATGCTGTCTACTATATAGCTTATGCTTTGCACATTGAGTTTAAGAATAAGGTCAAAACTGCTAATGAGATCTTCAATCTTGGAATCTCTAGGGATGCAAAGCCAGTGGAAAAGTTGAATGACGCGTACAAGAAGTTTATGGTGAGAACGATGAGAAGGTCCAACACAGCTGATGAAGAACCAAAGGAGAATAATGACTTACCGTCAAGAAGCTTTGGCACTTTATTGTCCAGGGGAGATAATAATGCAAGAAGGCAGGCGTTAGGAAGTTCTAACCCACAAGCCAAAAAGCTAAAGCCAAATCAATCATCCAAGACACCTTTTGCTATCTACGCAGATGCAGTTTCAGACACCACATCAGGGAATCAACCAGAGTCAGACAAGTCAAGACCAGAGTTTGGTAGTTGGCTCATGCTTGGAGGCAGAGCAGAGAGGAACAAAGAAAACAATTCTTTACCTAGAAAATGGGCATCATTCAAGGTTCCTCAGAAACCCATTGTGAGAACTGTTGCAGCAGCATCTGCTTCCACCTTTGAGGTTTTTGTCGATGAAGAAGAATGTACAGAAGAGGAAGAAGAAAAGAAGAAGAATGATGAAACTATCTCATCATCATCAAACGTTCTGCCCCTTAATGGCGGCCGTGAGATAAAAAAAGAAACAGAGCTGCTACGACAGAACCCTTTAAGACATTTCCCACCCAACAGCTTCCTACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009987 GO:0010369 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

395

Amino Acids

45.37

Weight (kDa)

6.75

Isoelectric Point (pI)

53.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 65 - 186 1.2e-43 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33560 AT2G33560
fragaria_vesca FvH4_5g26460 FvH4_5g26460
malus_domestica MD00G1178400.v1.1
prunus_persica Prupe.5G002900_v2.0.a1
pyrus_communis pycom06g00200
rosa_chinensis RchiOBHm_Chr7g0217371
rosa_laevigata RLG00000002485
rosa_multiflora Rmu_sc0011824.1_g000009
rosa_roxburghii Rroxscaffold_3G00242280
rosa_rugosa Rorug07G0168500
rosa_samantha Rh7AG308200 Rh7BG298900 Rh7CG327500 Rh7DG307300
rosa_wichuraiana Rw7G026130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 869
AccI GTMKAC 1 cut(s) 480
AccII CGCG 1 cut(s) 596
AciI CCGC 3 cut(s) 407, 425, 1107
AclI AACGTT 2 cut(s) 361, 1089
AclWI GGATC 1 cut(s) 26
AcoI YGGCCR 2 cut(s) 104, 1108
AcsI RAATTY 2 cut(s) 202, 344
AfaI GTAC 3 cut(s) 276, 599, 1033
AfiI CCNNNNNNNGG 4 cut(s) 296, 831, 869, 1103
AflIII ACRYGT 1 cut(s) 594
AgsI TTSAA 6 cut(s) 91, 112, 212, 547, 589, 949
AhdI GACNNNNNGTC 1 cut(s) 673
AjnI CCWGG 2 cut(s) 292, 701
AluBI AGCT 7 cut(s) 85, 491, 641, 684, 763, 1135, 1176
AluI AGCT 7 cut(s) 85, 491, 641, 684, 763, 1135, 1176
Alw26I GTCTC 1 cut(s) 59
AlwI GGATC 1 cut(s) 26
AlwNI CAGNNNCTG 1 cut(s) 992
AoxI GGCC 2 cut(s) 104, 1108
ApeKI GCWGC 4 cut(s) 5, 983, 986, 1135
ApoI RAATTY 2 cut(s) 202, 344
AspLEI GCGC 1 cut(s) 419
AspS9I GGNCC 1 cut(s) 630
AsuHPI GGTGA 1 cut(s) 625
AsuII TTCGAA 1 cut(s) 56
AvaII GGWCC 1 cut(s) 630
BbvI GCAGC 4 cut(s) 17, 995, 998, 1122
BceAI ACGGC 1 cut(s) 1095
BcgI CGANNNNNNTGC 2 cut(s) 320, 354
BciT130I CCWGG 2 cut(s) 294, 703
BciVI GTATCC 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 59
BfaI CTAG 2 cut(s) 561, 930
BfuI GTATCC 1 cut(s) 65
BglII AGATCT 1 cut(s) 540
BisI GCNGC 5 cut(s) 6, 984, 987, 1108, 1136
BlsI GCNGC 5 cut(s) 7, 985, 988, 1109, 1137
Bme1390I CCNGG 2 cut(s) 294, 703
Bme18I GGWCC 1 cut(s) 630
BmeRI GACNNNNNGTC 1 cut(s) 673
BmgT120I GGNCC 1 cut(s) 630
BmiI GGNNCC 1 cut(s) 954
BmrFI CCNGG 2 cut(s) 294, 703
BmsI GCATC 5 cut(s) 412, 556, 799, 950, 998
Bpu14I TTCGAA 1 cut(s) 56
BsaJI CCNNGG 2 cut(s) 251, 702
Bsc4I CCNNNNNNNGG 4 cut(s) 296, 831, 869, 1103
Bse1I ACTGG 2 cut(s) 73, 575
Bse3DI GCAATG 3 cut(s) 130, 338, 412
BseBI CCWGG 2 cut(s) 294, 703
BseDI CCNNGG 2 cut(s) 251, 702
BseGI GGATG 3 cut(s) 376, 571, 779
BseLI CCNNNNNNNGG 4 cut(s) 296, 831, 869, 1103
BseMI GCAATG 3 cut(s) 130, 338, 412
BseMII CTCAG 1 cut(s) 971
BseNI ACTGG 2 cut(s) 73, 575
BseRI GAGGAG 1 cut(s) 312
BseX3I CGGCCG 1 cut(s) 1108
BseXI GCAGC 4 cut(s) 17, 995, 998, 1122
Bsh1236I CGCG 1 cut(s) 596
Bsh1285I CGRYCG 1 cut(s) 1111
BshFI GGCC 2 cut(s) 106, 1110
BsiEI CGRYCG 1 cut(s) 1111
BsiSI CCGG 1 cut(s) 103
BslI CCNNNNNNNGG 4 cut(s) 296, 831, 869, 1103
BsmAI GTCTC 1 cut(s) 59
BsnI GGCC 2 cut(s) 106, 1110
Bsp119I TTCGAA 1 cut(s) 56
Bsp1407I TGTACA 1 cut(s) 1031
Bsp143I GATC 3 cut(s) 31, 373, 540
Bsp19I CCATGG 1 cut(s) 251
BspACI CCGC 3 cut(s) 407, 425, 1107
BspANI GGCC 2 cut(s) 106, 1110
BspCNI CTCAG 1 cut(s) 970
BspFNI CGCG 1 cut(s) 596
BspLI GGNNCC 1 cut(s) 954
BspPI GGATC 1 cut(s) 26
BspT104I TTCGAA 1 cut(s) 56
BsrDI GCAATG 3 cut(s) 130, 338, 412
BsrGI TGTACA 1 cut(s) 1031
BsrI ACTGG 2 cut(s) 73, 575
BssECI CCNNGG 2 cut(s) 251, 702
BssMI GATC 3 cut(s) 31, 373, 540
BssT1I CCWWGG 1 cut(s) 251
Bst2UI CCWGG 2 cut(s) 294, 703
Bst4CI ACNGT 3 cut(s) 315, 675, 979
Bst6I CTCTTC 2 cut(s) 107, 1032
BstAPI GCANNNNNTGC 1 cut(s) 992
BstAUI TGTACA 1 cut(s) 1031
BstBI TTCGAA 1 cut(s) 56
BstC8I GCNNGC 1 cut(s) 729
BstDEI CTNAG 1 cut(s) 957
BstDSI CCRYGG 1 cut(s) 251
BstF5I GGATG 3 cut(s) 376, 571, 779
BstFNI CGCG 1 cut(s) 596
BstHHI GCGC 1 cut(s) 419
BstKTI GATC 3 cut(s) 34, 376, 543
BstMAI GTCTC 1 cut(s) 59
BstMBI GATC 3 cut(s) 31, 373, 540
BstMCI CGRYCG 1 cut(s) 1111
BstMWI GCNNNNNNNGC 2 cut(s) 803, 992
BstNI CCWGG 2 cut(s) 294, 703
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 2 cut(s) 292, 701
BstUI CGCG 1 cut(s) 596
BstV1I GCAGC 4 cut(s) 17, 995, 998, 1122
BstX2I RGATCY 2 cut(s) 31, 540
BstYI RGATCY 2 cut(s) 31, 540
BstZI CGGCCG 1 cut(s) 1108
BsuI GTATCC 1 cut(s) 65
BsuRI GGCC 2 cut(s) 106, 1110
BtgI CCRYGG 1 cut(s) 251
BtsCI GGATG 3 cut(s) 376, 571, 779
BtsIMutI CAGTG 1 cut(s) 582
Cac8I GCNNGC 1 cut(s) 729
CaiI CAGNNNCTG 1 cut(s) 992
CfoI GCGC 1 cut(s) 419
Cfr13I GGNCC 1 cut(s) 630
CseI GACGC 1 cut(s) 602
Csp6I GTAC 3 cut(s) 275, 598, 1032
CspCI CAANNNNNGTGG 2 cut(s) 558, 593
CviAII CATG 3 cut(s) 252, 473, 883
CviQI GTAC 3 cut(s) 275, 598, 1032
DdeI CTNAG 1 cut(s) 957
DpnI GATC 3 cut(s) 33, 375, 542
DpnII GATC 3 cut(s) 31, 373, 540
DriI GACNNNNNGTC 1 cut(s) 673
EaeI YGGCCR 2 cut(s) 104, 1108
EagI CGGCCG 1 cut(s) 1108
Eam1104I CTCTTC 2 cut(s) 107, 1032
Eam1105I GACNNNNNGTC 1 cut(s) 673
EarI CTCTTC 2 cut(s) 107, 1032
EclXI CGGCCG 1 cut(s) 1108
Eco130I CCWWGG 1 cut(s) 251
Eco47I GGWCC 1 cut(s) 630
Eco52I CGGCCG 1 cut(s) 1108
EcoRII CCWGG 2 cut(s) 292, 701
EcoT14I CCWWGG 1 cut(s) 251
ErhI CCWWGG 1 cut(s) 251
FaeI CATG 3 cut(s) 255, 476, 886
FatI CATG 3 cut(s) 251, 472, 882
FblI GTMKAC 1 cut(s) 480
Fnu4HI GCNGC 5 cut(s) 6, 984, 987, 1108, 1136
FokI GGATG 3 cut(s) 383, 578, 766
Fsp4HI GCNGC 5 cut(s) 6, 984, 987, 1108, 1136
FspBI CTAG 2 cut(s) 561, 930
GlaI GCGC 1 cut(s) 418
GluI GCNGC 5 cut(s) 6, 984, 987, 1108, 1136
HaeIII GGCC 2 cut(s) 106, 1110
HapII CCGG 1 cut(s) 103
HgaI GACGC 1 cut(s) 602
HhaI GCGC 1 cut(s) 419
Hin1II CATG 3 cut(s) 255, 476, 886
Hin6I GCGC 1 cut(s) 417
HinP1I GCGC 1 cut(s) 417
HindIII AAGCTT 1 cut(s) 682
HinfI GANTC 4 cut(s) 181, 555, 835, 845
HpaII CCGG 1 cut(s) 103
HphI GGTGA 1 cut(s) 625
Hpy166II GTNNAC 1 cut(s) 481
Hpy188I TCNGA 6 cut(s) 31, 163, 358, 820, 850, 960
Hpy188III TCNNGA 5 cut(s) 35, 190, 309, 678, 858
Hpy8I GTNNAC 1 cut(s) 481
HpyAV CCTTC 4 cut(s) 10, 224, 621, 717
HpyCH4III ACNGT 3 cut(s) 315, 675, 979
HpyCH4IV ACGT 3 cut(s) 99, 361, 1089
HpyCH4V TGCA 5 cut(s) 502, 569, 719, 812, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 803, 992
HpyF3I CTNAG 1 cut(s) 957
HpySE526I ACGT 3 cut(s) 99, 361, 1089
Hsp92II CATG 3 cut(s) 255, 476, 886
HspAI GCGC 1 cut(s) 417
Kzo9I GATC 3 cut(s) 31, 373, 540
LmnI GCTCC 2 cut(s) 82, 409
Lsp1109I GCAGC 4 cut(s) 17, 995, 998, 1122
LweI GCATC 5 cut(s) 412, 556, 799, 950, 998
MaeI CTAG 2 cut(s) 561, 930
MaeII ACGT 3 cut(s) 99, 361, 1089
MaeIII GTNAC 1 cut(s) 362
MalI GATC 3 cut(s) 33, 375, 542
MboI GATC 3 cut(s) 31, 373, 540
MfeI CAATTG 1 cut(s) 170
MflI RGATCY 2 cut(s) 31, 540
MluCI AATT 5 cut(s) 52, 170, 202, 344, 919
MluI ACGCGT 1 cut(s) 594
MlyI GAGTC 1 cut(s) 854
MmeI TCCRAC 2 cut(s) 378, 657
MseI TTAA 5 cut(s) 51, 387, 513, 1101, 1154
MspA1I CMGCKG 1 cut(s) 641
MspI CCGG 1 cut(s) 103
MspR9I CCNGG 2 cut(s) 294, 703
MunI CAATTG 1 cut(s) 170
MvaI CCWGG 2 cut(s) 294, 703
MvnI CGCG 1 cut(s) 596
MwoI GCNNNNNNNGC 2 cut(s) 803, 992
NcoI CCATGG 1 cut(s) 251
NdeII GATC 3 cut(s) 31, 373, 540
NlaIII CATG 3 cut(s) 255, 476, 886
NlaIV GGNNCC 1 cut(s) 954
NspI RCATGY 1 cut(s) 476
NspV TTCGAA 1 cut(s) 56
PfeI GAWTC 3 cut(s) 181, 555, 835
PflMI CCANNNNNTGG 1 cut(s) 869
PkrI GCNGC 5 cut(s) 7, 985, 988, 1109, 1137
PleI GAGTC 1 cut(s) 853
PpsI GAGTC 1 cut(s) 853
Psp1406I AACGTT 2 cut(s) 361, 1089
Psp6I CCWGG 2 cut(s) 292, 701
PspGI CCWGG 2 cut(s) 292, 701
PspN4I GGNNCC 1 cut(s) 954
PspPI GGNCC 1 cut(s) 630
PstNI CAGNNNCTG 1 cut(s) 992
PsuI RGATCY 2 cut(s) 31, 540
PvuII CAGCTG 1 cut(s) 641
RsaI GTAC 3 cut(s) 276, 599, 1033
RsaNI GTAC 3 cut(s) 275, 598, 1032
SaqAI TTAA 5 cut(s) 51, 387, 513, 1101, 1154
SatI GCNGC 5 cut(s) 6, 984, 987, 1108, 1136
Sau3AI GATC 3 cut(s) 31, 373, 540
Sau96I GGNCC 1 cut(s) 630
SchI GAGTC 1 cut(s) 854
ScrFI CCNGG 2 cut(s) 294, 703
SfaNI GCATC 5 cut(s) 412, 556, 799, 950, 998
SfuI TTCGAA 1 cut(s) 56
SinI GGWCC 1 cut(s) 630
Sse9I AATT 5 cut(s) 52, 170, 202, 344, 919
SsiI CCGC 3 cut(s) 407, 425, 1107
SspMI CTAG 2 cut(s) 561, 930
StyD4I CCNGG 2 cut(s) 292, 701
StyI CCWWGG 1 cut(s) 251
TaaI ACNGT 3 cut(s) 315, 675, 979
TaiI ACGT 3 cut(s) 102, 364, 1092
TaqI TCGA 3 cut(s) 56, 189, 1017
TasI AATT 5 cut(s) 52, 170, 202, 344, 919
TatI WGTACW 1 cut(s) 1031
TauI GCSGC 1 cut(s) 1110
TfiI GAWTC 3 cut(s) 181, 555, 835
Tru1I TTAA 5 cut(s) 51, 387, 513, 1101, 1154
Tru9I TTAA 5 cut(s) 51, 387, 513, 1101, 1154
TscAI CASTG 1 cut(s) 582
TseI GCWGC 4 cut(s) 5, 983, 986, 1135
TspDTI ATGAA 4 cut(s) 243, 660, 1035, 1080
TspRI CASTG 1 cut(s) 582
Van91I CCANNNNNTGG 1 cut(s) 869
VpaK11BI GGWCC 1 cut(s) 630
XapI RAATTY 2 cut(s) 202, 344
XceI RCATGY 1 cut(s) 476
XmiI GTMKAC 1 cut(s) 480
XspI CTAG 2 cut(s) 561, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.