RchiOBHm_Chr7g0217371

Mitotic spindle checkpoint protein BUBR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014113
Physical Location & Seq
Reverse (-)
35235537 .. 35239715
4179 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19453

Sequence Viewer

Length: 1167 bp
ATGGCGGAAAGTGGAATTGAAATAGAGAAAATAGAGATGTTGGATCCAGAGTCAGAGTTCCTGGCGTCGAATAGGGAGTCAGGGAACGAGTGGGAGCTGTTCAAGGAGAACGTGCGGCCACTGAAGAGAGGCCGCAAAGTTGAAATCTTAAACGACGCTCTCAAAGCTCAAACTGACAACCAACTCAAGAAGTCCCTCTTTGAACAACGAAGGCGGTTGATTCAAGCAATTGACGAGTATGAAGGAGATGACCCTCTGCAGCCGTGGCTCGAGTGTATCAAGTGGGTTCAGGAAGCATTCCCCCCTGGTGGGGATTTCTCGGGACTGGTTGTGATATATGAGCAATGTGTGCGTACATTTTGGCATTCAGACCGCTACAAGGATGATCTTCGTTATCTCAAAGTCTGGTTGGAATATGCTGAAAATTGTGCGGATGCTGAAGTCATATTCAGTTTTCTAGATGCAAATGAAATTGGAAAGACACATTCTCAGTATTACATATCTTATGCTTTGTTGATGGAATCCAAGAACAAAGTGAAAGCTGCCAATGAAATATTAAATCTTGGGATATCAAGGAATGCTCAGCCCACAGAAAAATTGAATGATGCATTTAGGAAATTTCTTGCACGCTCAATGAGAAGACCACCAAAAGCTCCAGAGGAGGATTCAGCTGAGAAACGCTTACCCACCCGAAGCTTTGGAACTGTCCTTGCCAAGGGAGAGAATCGTCTGCAGACAAGTGAGAGCTCTGGTTTGTTGAAAAAGAACGTGAAGCTAGATAGGGGTCGTGTTCCACTTAGCATTTATAAAGAAGACTCAGATTCCCTTGACCAATCAGAAGTGTCAAAGAAAGACGTGAATTCTTGGCACACCCTTGGACCTAGAGCAGAGAGAAATAAAGAAAATAGTGCAATCCCTTCCAAGTGGACATCATATAAGGTTCGTCAAAGACCTGTGCCTAGAATTGGAGGAGCAGCTGCTACTAGTGCCTGCATTGAGATTTTTGTTGATGAAGAATGTGAAGAAACACATAGAATGAATGCCGAGGGTGCCAAATCTTCAACCTTGCAGCCTAGACAGGGTGAAGAGCAAGAGCTCAAGAAGGAAACAGAGTTGTTGAGACAGCAACCATTACGCAATTTCCCTCTAAATAGTCTTCCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009987 GO:0010369 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

388

Amino Acids

44.61

Weight (kDa)

6.05

Isoelectric Point (pI)

57.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 68 - 190 2.1e-43 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33560 AT2G33560
fragaria_vesca FvH4_5g26460 FvH4_5g26460
malus_domestica MD00G1178400.v1.1
prunus_persica Prupe.5G002900_v2.0.a1
pyrus_communis pycom06g00200
rosa_chinensis RchiOBHm_Chr7g0217371
rosa_laevigata RLG00000002485
rosa_multiflora Rmu_sc0011824.1_g000009
rosa_roxburghii Rroxscaffold_3G00242280
rosa_rugosa Rorug07G0168500
rosa_samantha Rh7AG308200 Rh7BG298900 Rh7CG327500 Rh7DG307300
rosa_wichuraiana Rw7G026130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 807
AccB1I GGYRCC 1 cut(s) 1049
AciI CCGC 6 cut(s) 5, 115, 133, 214, 373, 431
AclWI GGATC 2 cut(s) 38, 51
AcoI YGGCCR 1 cut(s) 116
AcsI RAATTY 2 cut(s) 617, 859
AcuI CTGAAG 2 cut(s) 143, 459
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 1 cut(s) 355
AfiI CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 965, 1079
AgsI TTSAA 8 cut(s) 20, 103, 143, 203, 224, 601, 760, 1062
AhlI ACTAGT 1 cut(s) 983
AjiI CACGTC 1 cut(s) 856
AjnI CCWGG 2 cut(s) 60, 304
Alw21I GWGCWC 2 cut(s) 749, 1098
Alw26I GTCTC 1 cut(s) 1114
AlwI GGATC 2 cut(s) 38, 51
Ama87I CYCGRG 2 cut(s) 269, 319
AoxI GGCC 2 cut(s) 116, 130
ApeKI GCWGC 5 cut(s) 259, 542, 974, 977, 1069
ApoI RAATTY 2 cut(s) 617, 859
ArsI GACNNNNNNTTYG 2 cut(s) 62, 94
AspS9I GGNCC 1 cut(s) 878
AsuHPI GGTGA 1 cut(s) 1094
AvaI CYCGRG 2 cut(s) 269, 319
AvaII GGWCC 1 cut(s) 878
BamHI GGATCC 1 cut(s) 43
BanI GGYRCC 1 cut(s) 1049
BanII GRGCYC 2 cut(s) 749, 1098
BbsI GAAGAC 3 cut(s) 646, 819, 1148
Bbv12I GWGCWC 2 cut(s) 749, 1098
BbvI GCAGC 5 cut(s) 271, 529, 964, 986, 1081
BccI CCATC 1 cut(s) 511
BceAI ACGGC 1 cut(s) 247
BciT130I CCWGG 2 cut(s) 62, 306
BcoDI GTCTC 1 cut(s) 1114
BcuI ACTAGT 1 cut(s) 983
BfaI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
BfmI CTRYAG 2 cut(s) 257, 731
BisI GCNGC 7 cut(s) 116, 133, 260, 543, 975, 978, 1070
BlpI GCTNAGC 1 cut(s) 582
BlsI GCNGC 7 cut(s) 117, 134, 261, 544, 976, 979, 1071
Bme1390I CCNGG 2 cut(s) 62, 306
Bme18I GGWCC 1 cut(s) 878
BmeT110I CYCGRG 2 cut(s) 269, 319
BmgBI CACGTC 1 cut(s) 856
BmgT120I GGNCC 1 cut(s) 878
BmiI GGNNCC 2 cut(s) 45, 1051
BmrFI CCNGG 2 cut(s) 62, 306
BmsI GCATC 3 cut(s) 424, 451, 595
BpiI GAAGAC 3 cut(s) 646, 819, 1148
BpmI CTGGAG 1 cut(s) 639
Bpu1102I GCTNAGC 1 cut(s) 582
BpuEI CTTGAG 2 cut(s) 170, 1082
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 5 cut(s) 263, 304, 714, 874, 1044
Bsc4I CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 965, 1079
Bse1I ACTGG 1 cut(s) 330
Bse3DI GCAATG 1 cut(s) 350
BseBI CCWGG 2 cut(s) 62, 306
BseDI CCNNGG 5 cut(s) 263, 304, 714, 874, 1044
BseGI GGATG 2 cut(s) 388, 439
BseLI CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 965, 1079
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 4 cut(s) 503, 596, 663, 831
BseNI ACTGG 1 cut(s) 330
BseRI GAGGAG 2 cut(s) 674, 984
BseXI GCAGC 5 cut(s) 271, 529, 964, 986, 1081
BshFI GGCC 2 cut(s) 118, 132
BshNI GGYRCC 1 cut(s) 1049
BsiHKAI GWGCWC 2 cut(s) 749, 1098
BsiHKCI CYCGRG 2 cut(s) 269, 319
BslFI GGGAC 2 cut(s) 178, 336
BslI CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 965, 1079
BsmAI GTCTC 1 cut(s) 1114
BsmFI GGGAC 2 cut(s) 178, 336
BsmI GAATGC 4 cut(s) 296, 364, 583, 1045
BsnI GGCC 2 cut(s) 118, 132
BsoBI CYCGRG 2 cut(s) 269, 319
Bsp1286I GDGCHC 2 cut(s) 749, 1098
Bsp143I GATC 2 cut(s) 43, 385
Bsp1720I GCTNAGC 1 cut(s) 582
BspACI CCGC 6 cut(s) 5, 115, 133, 214, 373, 431
BspANI GGCC 2 cut(s) 118, 132
BspCNI CTCAG 4 cut(s) 502, 595, 664, 830
BspLI GGNNCC 2 cut(s) 45, 1051
BspMAI CTGCAG 2 cut(s) 261, 735
BspPI GGATC 2 cut(s) 38, 51
BspQI GCTCTTC 1 cut(s) 1080
BspT107I GGYRCC 1 cut(s) 1049
BsrDI GCAATG 1 cut(s) 350
BsrI ACTGG 1 cut(s) 330
BssECI CCNNGG 5 cut(s) 263, 304, 714, 874, 1044
BssMI GATC 2 cut(s) 43, 385
BssNI GRCGYC 1 cut(s) 65
BssT1I CCWWGG 2 cut(s) 714, 874
Bst2UI CCWGG 2 cut(s) 62, 306
Bst4CI ACNGT 1 cut(s) 706
Bst6I CTCTTC 2 cut(s) 119, 1080
BstACI GRCGYC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 628, 991
BstDEI CTNAG 5 cut(s) 489, 582, 672, 797, 817
BstDSI CCRYGG 1 cut(s) 263
BstENI CCTNNNNNAGG 1 cut(s) 713
BstF5I GGATG 2 cut(s) 388, 439
BstKTI GATC 2 cut(s) 46, 388
BstMAI GTCTC 1 cut(s) 1114
BstMBI GATC 2 cut(s) 43, 385
BstMWI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 986, 1049
BstNI CCWGG 2 cut(s) 62, 306
BstSCI CCNGG 2 cut(s) 60, 304
BstSFI CTRYAG 2 cut(s) 257, 731
BstV1I GCAGC 5 cut(s) 271, 529, 964, 986, 1081
BstV2I GAAGAC 3 cut(s) 646, 819, 1148
BstX2I RGATCY 1 cut(s) 43
BstYI RGATCY 1 cut(s) 43
BsuRI GGCC 2 cut(s) 118, 132
BtgI CCRYGG 1 cut(s) 263
BtrI CACGTC 1 cut(s) 856
BtsCI GGATG 2 cut(s) 388, 439
BtsIMutI CAGTG 1 cut(s) 119
Cac8I GCNNGC 2 cut(s) 628, 991
Cfr13I GGNCC 1 cut(s) 878
CseI GACGC 2 cut(s) 54, 164
Csp6I GTAC 1 cut(s) 354
CviQI GTAC 1 cut(s) 354
DdeI CTNAG 5 cut(s) 489, 582, 672, 797, 817
DpnI GATC 2 cut(s) 45, 387
DpnII GATC 2 cut(s) 43, 385
EaeI YGGCCR 1 cut(s) 116
Eam1104I CTCTTC 2 cut(s) 119, 1080
EarI CTCTTC 2 cut(s) 119, 1080
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 747, 1096
Eco130I CCWWGG 2 cut(s) 714, 874
Eco24I GRGCYC 2 cut(s) 749, 1098
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 1 cut(s) 878
Eco53kI GAGCTC 2 cut(s) 747, 1096
Eco57I CTGAAG 2 cut(s) 143, 459
Eco88I CYCGRG 2 cut(s) 269, 319
EcoICRI GAGCTC 2 cut(s) 747, 1096
EcoNI CCTNNNNNAGG 1 cut(s) 713
EcoRI GAATTC 1 cut(s) 859
EcoRII CCWGG 2 cut(s) 60, 304
EcoRV GATATC 1 cut(s) 570
EcoT14I CCWWGG 2 cut(s) 714, 874
EcoT22I ATGCAT 1 cut(s) 610
EcoT38I GRGCYC 2 cut(s) 749, 1098
ErhI CCWWGG 2 cut(s) 714, 874
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 2 cut(s) 178, 336
Fnu4HI GCNGC 7 cut(s) 116, 133, 260, 543, 975, 978, 1070
FokI GGATG 2 cut(s) 395, 446
FriOI GRGCYC 2 cut(s) 749, 1098
Fsp4HI GCNGC 7 cut(s) 116, 133, 260, 543, 975, 978, 1070
FspBI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
GluI GCNGC 7 cut(s) 116, 133, 260, 543, 975, 978, 1070
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 2 cut(s) 118, 132
HgaI GACGC 2 cut(s) 54, 164
Hin1I GRCGYC 1 cut(s) 65
HindIII AAGCTT 1 cut(s) 694
HinfI GANTC 8 cut(s) 50, 77, 220, 521, 665, 724, 815, 821
HphI GGTGA 1 cut(s) 1094
Hpy166II GTNNAC 1 cut(s) 927
Hpy188I TCNGA 4 cut(s) 55, 370, 820, 838
Hpy188III TCNNGA 7 cut(s) 47, 187, 290, 321, 458, 656, 1099
Hpy8I GTNNAC 1 cut(s) 927
Hpy99I CGWCG 2 cut(s) 70, 158
HpyAV CCTTC 4 cut(s) 204, 236, 927, 1096
HpyCH4III ACNGT 1 cut(s) 706
HpyCH4IV ACGT 3 cut(s) 111, 768, 855
HpyCH4V TGCA 8 cut(s) 259, 464, 608, 626, 733, 911, 993, 1069
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 986, 1049
HpyF3I CTNAG 5 cut(s) 489, 582, 672, 797, 817
HpySE526I ACGT 3 cut(s) 111, 768, 855
Hsp92I GRCGYC 1 cut(s) 65
Kzo9I GATC 2 cut(s) 43, 385
LguI GCTCTTC 1 cut(s) 1080
LmnI GCTCC 3 cut(s) 94, 658, 971
Lsp1109I GCAGC 5 cut(s) 271, 529, 964, 986, 1081
LweI GCATC 3 cut(s) 424, 451, 595
MaeI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
MaeII ACGT 3 cut(s) 111, 768, 855
MalI GATC 2 cut(s) 45, 387
MboI GATC 2 cut(s) 43, 385
MboII GAAGA 9 cut(s) 136, 380, 651, 824, 1025, 1034, 1050, 1097, 1148
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 1 cut(s) 43
MhlI GDGCHC 2 cut(s) 749, 1098
MluCI AATT 9 cut(s) 15, 228, 424, 471, 596, 617, 859, 963, 1138
MlyI GAGTC 3 cut(s) 59, 86, 809
MmeI TCCRAC 2 cut(s) 21, 390
MnlI CCTC 8 cut(s) 122, 206, 264, 652, 655, 962, 1039, 1155
Mph1103I ATGCAT 1 cut(s) 610
MseI TTAA 2 cut(s) 149, 557
MspA1I CMGCKG 2 cut(s) 671, 977
MspR9I CCNGG 2 cut(s) 62, 306
MunI CAATTG 1 cut(s) 228
Mva1269I GAATGC 4 cut(s) 296, 364, 583, 1045
MvaI CCWGG 2 cut(s) 62, 306
MwoI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 986, 1049
NdeII GATC 2 cut(s) 43, 385
NlaIV GGNNCC 2 cut(s) 45, 1051
NmeAIII GCCGAG 1 cut(s) 1069
NsiI ATGCAT 1 cut(s) 610
PaeR7I CTCGAG 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 1080
PctI GAATGC 4 cut(s) 296, 364, 583, 1045
PfeI GAWTC 5 cut(s) 220, 521, 665, 724, 821
PkrI GCNGC 7 cut(s) 117, 134, 261, 544, 976, 979, 1071
PleI GAGTC 3 cut(s) 58, 85, 809
PpsI GAGTC 3 cut(s) 58, 85, 809
PsiI TTATAA 1 cut(s) 807
Psp124BI GAGCTC 2 cut(s) 749, 1098
Psp6I CCWGG 2 cut(s) 60, 304
PspGI CCWGG 2 cut(s) 60, 304
PspN4I GGNNCC 2 cut(s) 45, 1051
PspPI GGNCC 1 cut(s) 878
PspXI VCTCGAGB 1 cut(s) 269
PstI CTGCAG 2 cut(s) 261, 735
PsuI RGATCY 1 cut(s) 43
PvuII CAGCTG 2 cut(s) 671, 977
RsaI GTAC 1 cut(s) 355
RsaNI GTAC 1 cut(s) 354
SacI GAGCTC 2 cut(s) 749, 1098
SapI GCTCTTC 1 cut(s) 1080
SaqAI TTAA 2 cut(s) 149, 557
SatI GCNGC 7 cut(s) 116, 133, 260, 543, 975, 978, 1070
Sau3AI GATC 2 cut(s) 43, 385
Sau96I GGNCC 1 cut(s) 878
SchI GAGTC 3 cut(s) 59, 86, 809
ScrFI CCNGG 2 cut(s) 62, 306
SduI GDGCHC 2 cut(s) 749, 1098
SfaNI GCATC 3 cut(s) 424, 451, 595
SfcI CTRYAG 2 cut(s) 257, 731
Sfr274I CTCGAG 1 cut(s) 269
SinI GGWCC 1 cut(s) 878
SlaI CTCGAG 1 cut(s) 269
SmlI CTYRAG 3 cut(s) 185, 269, 1097
SmoI CTYRAG 3 cut(s) 185, 269, 1097
SpeI ACTAGT 1 cut(s) 983
Sse9I AATT 9 cut(s) 15, 228, 424, 471, 596, 617, 859, 963, 1138
SsiI CCGC 6 cut(s) 5, 115, 133, 214, 373, 431
SspI AATATT 1 cut(s) 555
SspMI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
SstI GAGCTC 2 cut(s) 749, 1098
StyD4I CCNGG 2 cut(s) 60, 304
StyI CCWWGG 2 cut(s) 714, 874
TaaI ACNGT 1 cut(s) 706
TaiI ACGT 3 cut(s) 114, 771, 858
TaqI TCGA 2 cut(s) 68, 270
TasI AATT 9 cut(s) 15, 228, 424, 471, 596, 617, 859, 963, 1138
TauI GCSGC 2 cut(s) 118, 135
TfiI GAWTC 5 cut(s) 220, 521, 665, 724, 821
Tru1I TTAA 2 cut(s) 149, 557
Tru9I TTAA 2 cut(s) 149, 557
TscAI CASTG 1 cut(s) 126
TseI GCWGC 5 cut(s) 259, 542, 974, 977, 1069
TspDTI ATGAA 5 cut(s) 255, 483, 564, 1026, 1052
TspRI CASTG 1 cut(s) 126
VpaK11BI GGWCC 1 cut(s) 878
XagI CCTNNNNNAGG 1 cut(s) 713
XapI RAATTY 2 cut(s) 617, 859
XbaI TCTAGA 1 cut(s) 457
XhoI CTCGAG 1 cut(s) 269
XspI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.