pycom06g00200

Mitotic spindle checkpoint protein BUBR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0014113
Physical Location & Seq
Forward (+)
214359 .. 217455
3097 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g00200.2

Sequence Viewer

Length: 1179 bp
ATGGCGGATAGTGGAATAGAGATAGAGAAAGTAGAGTGGTTGGATCCAGAGATGGAGTTTCTGGCATCGAAGCGAGAGACGGGGAATGAGTGGGAGCTCTTTAAGGAGAATGTTAGGCCCTTGAAGAGAGGCCGCAAAGTCTCCATCTTAAACGAAGCTCTCAAGGTTCACAACGACAATCAACTCAAGATATCCCTCCTTGACCAAAGAAGGCTGTTGATTCAAGCGATTGACGAGTATAAAGGCGAGGATCCTCTCCACCCATGGCTTGAGTGTATCAAGTGGGTTCAGGAAGCATTTCCACCTGGTGGAGATTCCTCGGGGTTGGTGGTGATATATGAGCAATGCGTACGTACATTTTGGCATTCAGACCGTTACAAGGATGATCTCCGCTATCTCAAAGTCTGGTTGGAATATGCTGAAAATTGTGTAGATGCTCAAGTCATATACAGTTTTCTTGATGCAAATGAAATCGGGAAGACACATGCGGTTTTCTACATCTCATATGCTTTGCTCTTGGAATCCAAGAACAAAATGAAAGCTGCCAATGAAACATTTAATCTTGGGATATCTAGGAATGCTCAGCCCGTAGAAAAATTGAAGGATGCATATAGGAAATTTATTGCTCGCTCTATGAGAAAATCAAAAGCTGCAGAGGAGGATCCAATGGAGAAACATTTACCCACCCGAAGCTTTGGAACTGTCCTTTCCAAGGGAGAGAATCGAAAGCTGGGGAGCTCTGATCTGCTGAGAAATAATTTGAAGCCAGATAGGGATCGTGTCCTGGCTCCAATCAACATTTATAAAGAGTCAAATTCTGTTGATGTTGGCTCTCGTTACCATCCAGACGTGCCAAAGACAGACCTGAATTCTTGGCACACCCTTGGACCTAGAGCTGAGAGAAATAAAGAAAACAATGCTATCCCTACCAAGTGGACGACATACAAGATTCCACAGAGACCTGGATCTAGAACTAGAGGAGCAGGTGCTAGTAGCACCTGTATTGAAGTTTTTGTTGATGAAGAATGTGAAGAGAAACATAGAATGAATGTTGAGGAGGGTGCCAAGTCTTCAACCGTGCAACTTAGACTGGAGGAGGAGAGAGATCTCAAAAGGGAAACAGAATTGCTGCGGGAGGACCCCTTGCGCAATTTCCCTCCCAACAGTCTTCCTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009987 GO:0010369 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

393

Amino Acids

45.35

Weight (kDa)

6.75

Isoelectric Point (pI)

41.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33560 AT2G33560
fragaria_vesca FvH4_5g26460 FvH4_5g26460
malus_domestica MD00G1178400.v1.1
prunus_persica Prupe.5G002900_v2.0.a1
pyrus_communis pycom06g00200
rosa_chinensis RchiOBHm_Chr7g0217371
rosa_laevigata RLG00000002485
rosa_multiflora Rmu_sc0011824.1_g000009
rosa_roxburghii Rroxscaffold_3G00242280
rosa_rugosa Rorug07G0168500
rosa_samantha Rh7AG308200 Rh7BG298900 Rh7CG327500 Rh7DG307300
rosa_wichuraiana Rw7G026130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 804
AarI CACCTGC 1 cut(s) 974
Acc16I TGCGCA 1 cut(s) 1148
Acc36I ACCTGC 1 cut(s) 974
AccB1I GGYRCC 1 cut(s) 1061
AccB7I CCANNNNNTGG 1 cut(s) 308
AciI CCGC 5 cut(s) 5, 133, 391, 488, 1132
AclWI GGATC 8 cut(s) 38, 51, 245, 258, 656, 669, 783, 973
AcsI RAATTY 3 cut(s) 617, 814, 868
AdeI CACNNNGTG 1 cut(s) 308
AfaI GTAC 2 cut(s) 351, 355
AfiI CCNNNNNNNGG 3 cut(s) 308, 379, 712
AgsI TTSAA 6 cut(s) 124, 224, 601, 763, 1007, 1074
AjiI CACGTC 1 cut(s) 850
AjnI CCWGG 3 cut(s) 304, 783, 961
AluBI AGCT 8 cut(s) 97, 158, 542, 650, 693, 730, 738, 896
AluI AGCT 8 cut(s) 97, 158, 542, 650, 693, 730, 738, 896
Alw21I GWGCWC 2 cut(s) 99, 740
Alw26I GTCTC 3 cut(s) 71, 145, 952
AlwI GGATC 8 cut(s) 38, 51, 245, 258, 656, 669, 783, 973
Ama87I CYCGRG 1 cut(s) 319
AoxI GGCC 2 cut(s) 116, 130
ApeKI GCWGC 3 cut(s) 542, 650, 1129
ApoI RAATTY 3 cut(s) 617, 814, 868
Asp700I GAANNNNTTC 1 cut(s) 297
AspA2I CCTAGG 1 cut(s) 1172
AspLEI GCGC 1 cut(s) 1149
AspS9I GGNCC 3 cut(s) 117, 887, 1138
AsuHPI GGTGA 1 cut(s) 343
AvaI CYCGRG 1 cut(s) 319
AvaII GGWCC 2 cut(s) 887, 1138
AvrII CCTAGG 1 cut(s) 1172
BamHI GGATCC 3 cut(s) 43, 250, 661
BanI GGYRCC 1 cut(s) 1061
BanII GRGCYC 2 cut(s) 99, 740
BbsI GAAGAC 3 cut(s) 485, 1062, 1160
Bbv12I GWGCWC 2 cut(s) 99, 740
BbvI GCAGC 3 cut(s) 529, 637, 1116
BccI CCATC 3 cut(s) 46, 152, 849
BciT130I CCWGG 3 cut(s) 306, 785, 963
BcoDI GTCTC 3 cut(s) 71, 145, 952
BfaI CTAG 6 cut(s) 573, 891, 969, 975, 990, 1173
BfmI CTRYAG 1 cut(s) 651
BfuAI ACCTGC 1 cut(s) 974
BglII AGATCT 1 cut(s) 1105
BisI GCNGC 4 cut(s) 133, 543, 651, 1130
BlnI CCTAGG 1 cut(s) 1172
BlpI GCTNAGC 1 cut(s) 582
BlsI GCNGC 4 cut(s) 134, 544, 652, 1131
Bme1390I CCNGG 3 cut(s) 306, 785, 963
Bme18I GGWCC 2 cut(s) 887, 1138
BmeT110I CYCGRG 1 cut(s) 319
BmgBI CACGTC 1 cut(s) 850
BmgT120I GGNCC 3 cut(s) 117, 887, 1138
BmiI GGNNCC 6 cut(s) 45, 252, 663, 789, 1063, 1140
BmrFI CCNGG 3 cut(s) 306, 785, 963
BmsI GCATC 4 cut(s) 74, 424, 451, 595
BpiI GAAGAC 3 cut(s) 485, 1062, 1160
BpmI CTGGAG 1 cut(s) 1112
Bpu1102I GCTNAGC 1 cut(s) 582
BpuEI CTTGAG 4 cut(s) 146, 170, 290, 423
BsaAI YACGTR 1 cut(s) 353
BsaBI GATNNNNATC 1 cut(s) 774
BsaI GGTCTC 1 cut(s) 952
BsaJI CCNNGG 5 cut(s) 263, 318, 711, 883, 1172
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 379, 712
Bse1I ACTGG 1 cut(s) 1095
Bse3DI GCAATG 1 cut(s) 350
Bse8I GATNNNNATC 1 cut(s) 774
BseBI CCWGG 3 cut(s) 306, 785, 963
BseDI CCNNGG 5 cut(s) 263, 318, 711, 883, 1172
BseGI GGATG 3 cut(s) 388, 610, 841
BseJI GATNNNNATC 1 cut(s) 774
BseLI CCNNNNNNNGG 3 cut(s) 308, 379, 712
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 3 cut(s) 596, 740, 888
BseNI ACTGG 1 cut(s) 1095
BseRI GAGGAG 5 cut(s) 671, 993, 1070, 1109, 1112
BseXI GCAGC 3 cut(s) 529, 637, 1116
BseYI CCCAGC 1 cut(s) 730
BshFI GGCC 2 cut(s) 118, 132
BshNI GGYRCC 1 cut(s) 1061
BsiHKAI GWGCWC 2 cut(s) 99, 740
BsiHKCI CYCGRG 1 cut(s) 319
BsiWI CGTACG 1 cut(s) 349
BslI CCNNNNNNNGG 3 cut(s) 308, 379, 712
BsmAI GTCTC 3 cut(s) 71, 145, 952
BsmBI CGTCTC 1 cut(s) 71
BsmI GAATGC 2 cut(s) 364, 583
BsnI GGCC 2 cut(s) 118, 132
Bso31I GGTCTC 1 cut(s) 952
BsoBI CYCGRG 1 cut(s) 319
Bsp1286I GDGCHC 2 cut(s) 99, 740
Bsp143I GATC 8 cut(s) 43, 250, 385, 661, 742, 775, 965, 1105
Bsp1720I GCTNAGC 1 cut(s) 582
Bsp19I CCATGG 1 cut(s) 263
BspACI CCGC 5 cut(s) 5, 133, 391, 488, 1132
BspANI GGCC 2 cut(s) 118, 132
BspCNI CTCAG 3 cut(s) 595, 741, 889
BspLI GGNNCC 6 cut(s) 45, 252, 663, 789, 1063, 1140
BspMAI CTGCAG 1 cut(s) 655
BspMI ACCTGC 1 cut(s) 974
BspPI GGATC 8 cut(s) 38, 51, 245, 258, 656, 669, 783, 973
BspT107I GGYRCC 1 cut(s) 1061
BspTNI GGTCTC 1 cut(s) 952
BsrDI GCAATG 1 cut(s) 350
BsrI ACTGG 1 cut(s) 1095
BssECI CCNNGG 5 cut(s) 263, 318, 711, 883, 1172
BssMI GATC 8 cut(s) 43, 250, 385, 661, 742, 775, 965, 1105
BssT1I CCWWGG 4 cut(s) 263, 711, 883, 1172
Bst2UI CCWGG 3 cut(s) 306, 785, 963
Bst4CI ACNGT 5 cut(s) 374, 452, 703, 1078, 1166
Bst6I CTCTTC 2 cut(s) 119, 1026
BstBAI YACGTR 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 628
BstDEI CTNAG 4 cut(s) 582, 749, 897, 1085
BstDSI CCRYGG 1 cut(s) 263
BstENI CCTNNNNNAGG 1 cut(s) 710
BstF5I GGATG 3 cut(s) 388, 610, 841
BstHHI GCGC 1 cut(s) 1149
BstKTI GATC 8 cut(s) 46, 253, 388, 664, 745, 778, 968, 1108
BstMAI GTCTC 3 cut(s) 71, 145, 952
BstMBI GATC 8 cut(s) 43, 250, 385, 661, 742, 775, 965, 1105
BstNI CCWGG 3 cut(s) 306, 785, 963
BstNSI RCATGY 1 cut(s) 488
BstSCI CCNGG 3 cut(s) 304, 783, 961
BstSFI CTRYAG 1 cut(s) 651
BstSNI TACGTA 1 cut(s) 353
BstV1I GCAGC 3 cut(s) 529, 637, 1116
BstV2I GAAGAC 3 cut(s) 485, 1062, 1160
BstX2I RGATCY 5 cut(s) 43, 250, 661, 965, 1105
BstYI RGATCY 5 cut(s) 43, 250, 661, 965, 1105
BsuRI GGCC 2 cut(s) 118, 132
BtgI CCRYGG 1 cut(s) 263
BtrI CACGTC 1 cut(s) 850
BtsCI GGATG 3 cut(s) 388, 610, 841
BveI ACCTGC 1 cut(s) 974
Cac8I GCNNGC 1 cut(s) 628
CfoI GCGC 1 cut(s) 1149
Cfr13I GGNCC 3 cut(s) 117, 887, 1138
CsiI ACCWGGT 1 cut(s) 304
Csp6I GTAC 2 cut(s) 350, 354
CviAII CATG 2 cut(s) 264, 485
CviQI GTAC 2 cut(s) 350, 354
DdeI CTNAG 4 cut(s) 582, 749, 897, 1085
DpnI GATC 8 cut(s) 45, 252, 387, 663, 744, 777, 967, 1107
DpnII GATC 8 cut(s) 43, 250, 385, 661, 742, 775, 965, 1105
DraIII CACNNNGTG 1 cut(s) 308
Eam1104I CTCTTC 2 cut(s) 119, 1026
EarI CTCTTC 2 cut(s) 119, 1026
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 97, 738
Eco105I TACGTA 1 cut(s) 353
Eco130I CCWWGG 4 cut(s) 263, 711, 883, 1172
Eco24I GRGCYC 2 cut(s) 99, 740
Eco31I GGTCTC 1 cut(s) 952
Eco32I GATATC 2 cut(s) 192, 570
Eco47I GGWCC 2 cut(s) 887, 1138
Eco53kI GAGCTC 2 cut(s) 97, 738
Eco88I CYCGRG 1 cut(s) 319
EcoICRI GAGCTC 2 cut(s) 97, 738
EcoNI CCTNNNNNAGG 1 cut(s) 710
EcoO109I RGGNCCY 2 cut(s) 117, 1138
EcoRI GAATTC 1 cut(s) 868
EcoRII CCWGG 3 cut(s) 304, 783, 961
EcoRV GATATC 2 cut(s) 192, 570
EcoT14I CCWWGG 4 cut(s) 263, 711, 883, 1172
EcoT22I ATGCAT 1 cut(s) 610
EcoT38I GRGCYC 2 cut(s) 99, 740
ErhI CCWWGG 4 cut(s) 263, 711, 883, 1172
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 2 cut(s) 267, 488
FatI CATG 2 cut(s) 263, 484
FauI CCCGC 1 cut(s) 1125
FauNDI CATATG 1 cut(s) 505
Fnu4HI GCNGC 4 cut(s) 133, 543, 651, 1130
FokI GGATG 3 cut(s) 395, 617, 828
FriOI GRGCYC 2 cut(s) 99, 740
Fsp4HI GCNGC 4 cut(s) 133, 543, 651, 1130
FspBI CTAG 6 cut(s) 573, 891, 969, 975, 990, 1173
FspI TGCGCA 1 cut(s) 1148
GlaI GCGC 1 cut(s) 1148
GluI GCNGC 4 cut(s) 133, 543, 651, 1130
GsaI CCCAGC 1 cut(s) 734
GsuI CTGGAG 1 cut(s) 1112
HaeIII GGCC 2 cut(s) 118, 132
HhaI GCGC 1 cut(s) 1149
Hin1II CATG 2 cut(s) 267, 488
Hin6I GCGC 1 cut(s) 1147
HinP1I GCGC 1 cut(s) 1147
HindIII AAGCTT 1 cut(s) 691
HinfI GANTC 6 cut(s) 220, 314, 521, 721, 809, 949
HphI GGTGA 1 cut(s) 343
Hpy166II GTNNAC 2 cut(s) 169, 936
Hpy188I TCNGA 2 cut(s) 370, 742
Hpy188III TCNNGA 7 cut(s) 47, 187, 290, 458, 475, 845, 969
Hpy8I GTNNAC 2 cut(s) 169, 936
HpyAV CCTTC 2 cut(s) 204, 595
HpyCH4III ACNGT 5 cut(s) 374, 452, 703, 1078, 1166
HpyCH4IV ACGT 2 cut(s) 352, 849
HpyCH4V TGCA 4 cut(s) 464, 608, 653, 1081
HpyF3I CTNAG 4 cut(s) 582, 749, 897, 1085
HpySE526I ACGT 2 cut(s) 352, 849
Hsp92II CATG 2 cut(s) 267, 488
HspAI GCGC 1 cut(s) 1147
Kzo9I GATC 8 cut(s) 43, 250, 385, 661, 742, 775, 965, 1105
LmnI GCTCC 4 cut(s) 94, 735, 793, 980
Lsp1109I GCAGC 3 cut(s) 529, 637, 1116
LweI GCATC 4 cut(s) 74, 424, 451, 595
MabI ACCWGGT 1 cut(s) 304
MaeI CTAG 6 cut(s) 573, 891, 969, 975, 990, 1173
MaeII ACGT 2 cut(s) 352, 849
MaeIII GTNAC 2 cut(s) 374, 836
MalI GATC 8 cut(s) 45, 252, 387, 663, 744, 777, 967, 1107
MboI GATC 8 cut(s) 43, 250, 385, 661, 742, 775, 965, 1105
MboII GAAGA 6 cut(s) 136, 490, 1034, 1043, 1062, 1160
MflI RGATCY 5 cut(s) 43, 250, 661, 965, 1105
MhlI GDGCHC 2 cut(s) 99, 740
MluCI AATT 8 cut(s) 424, 596, 617, 757, 814, 868, 1124, 1150
MlyI GAGTC 1 cut(s) 818
MmeI TCCRAC 2 cut(s) 21, 390
Mph1103I ATGCAT 1 cut(s) 610
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 3 cut(s) 102, 149, 558
MspR9I CCNGG 3 cut(s) 306, 785, 963
Mva1269I GAATGC 2 cut(s) 364, 583
MvaI CCWGG 3 cut(s) 306, 785, 963
NcoI CCATGG 1 cut(s) 263
NdeI CATATG 1 cut(s) 505
NdeII GATC 8 cut(s) 43, 250, 385, 661, 742, 775, 965, 1105
NlaIII CATG 2 cut(s) 267, 488
NlaIV GGNNCC 6 cut(s) 45, 252, 663, 789, 1063, 1140
NsbI TGCGCA 1 cut(s) 1148
NsiI ATGCAT 1 cut(s) 610
NspI RCATGY 1 cut(s) 488
PaqCI CACCTGC 1 cut(s) 974
PctI GAATGC 2 cut(s) 364, 583
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 5 cut(s) 220, 314, 521, 721, 949
Pfl23II CGTACG 1 cut(s) 349
PflMI CCANNNNNTGG 1 cut(s) 308
PkrI GCNGC 4 cut(s) 134, 544, 652, 1131
PleI GAGTC 1 cut(s) 817
PpsI GAGTC 1 cut(s) 817
Ppu21I YACGTR 1 cut(s) 353
PpuMI RGGWCCY 1 cut(s) 1138
PsiI TTATAA 1 cut(s) 804
Psp124BI GAGCTC 2 cut(s) 99, 740
Psp5II RGGWCCY 1 cut(s) 1138
Psp6I CCWGG 3 cut(s) 304, 783, 961
PspFI CCCAGC 1 cut(s) 730
PspGI CCWGG 3 cut(s) 304, 783, 961
PspLI CGTACG 1 cut(s) 349
PspN4I GGNNCC 6 cut(s) 45, 252, 663, 789, 1063, 1140
PspPI GGNCC 3 cut(s) 117, 887, 1138
PspPPI RGGWCCY 1 cut(s) 1138
PstI CTGCAG 1 cut(s) 655
PsuI RGATCY 5 cut(s) 43, 250, 661, 965, 1105
RsaI GTAC 2 cut(s) 351, 355
RsaNI GTAC 2 cut(s) 350, 354
SacI GAGCTC 2 cut(s) 99, 740
SaqAI TTAA 3 cut(s) 102, 149, 558
SatI GCNGC 4 cut(s) 133, 543, 651, 1130
Sau3AI GATC 8 cut(s) 43, 250, 385, 661, 742, 775, 965, 1105
Sau96I GGNCC 3 cut(s) 117, 887, 1138
SchI GAGTC 1 cut(s) 818
ScrFI CCNGG 3 cut(s) 306, 785, 963
SduI GDGCHC 2 cut(s) 99, 740
SexAI ACCWGGT 1 cut(s) 304
SfaNI GCATC 4 cut(s) 74, 424, 451, 595
SfcI CTRYAG 1 cut(s) 651
SinI GGWCC 2 cut(s) 887, 1138
SmlI CTYRAG 4 cut(s) 161, 185, 269, 438
SmoI CTYRAG 4 cut(s) 161, 185, 269, 438
SnaBI TACGTA 1 cut(s) 353
Sse9I AATT 8 cut(s) 424, 596, 617, 757, 814, 868, 1124, 1150
SsiI CCGC 5 cut(s) 5, 133, 391, 488, 1132
SspMI CTAG 6 cut(s) 573, 891, 969, 975, 990, 1173
SstI GAGCTC 2 cut(s) 99, 740
StyD4I CCNGG 3 cut(s) 304, 783, 961
StyI CCWWGG 4 cut(s) 263, 711, 883, 1172
TaaI ACNGT 5 cut(s) 374, 452, 703, 1078, 1166
TaiI ACGT 2 cut(s) 355, 852
TaqI TCGA 2 cut(s) 68, 724
TasI AATT 8 cut(s) 424, 596, 617, 757, 814, 868, 1124, 1150
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 5 cut(s) 220, 314, 521, 721, 949
Tru1I TTAA 3 cut(s) 102, 149, 558
Tru9I TTAA 3 cut(s) 102, 149, 558
TseI GCWGC 3 cut(s) 542, 650, 1129
TspDTI ATGAA 5 cut(s) 483, 551, 564, 1035, 1061
Van91I CCANNNNNTGG 1 cut(s) 308
VpaK11BI GGWCC 2 cut(s) 887, 1138
XagI CCTNNNNNAGG 1 cut(s) 710
XapI RAATTY 3 cut(s) 617, 814, 868
XbaI TCTAGA 1 cut(s) 968
XceI RCATGY 1 cut(s) 488
XmaJI CCTAGG 1 cut(s) 1172
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 6 cut(s) 573, 891, 969, 975, 990, 1173
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.