FvH4_5g26460

Mitotic spindle checkpoint protein BUBR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014113
Physical Location & Seq
Reverse (-)
17937132 .. 17941585
4454 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26460.t1

Sequence Viewer

Length: 1167 bp
ATGGCGGACAGTGGAATTGAAATAGAGAAAATAGAGGTTTTGGATCCAGAATCCGAGTTCTTGGCGTCGAATCGGGAGACGGGGAACGAGTGGGAGCTGTTCAAGGAGAACGTGAGGCCGTTGAAGAGAGGCCGCAAAGTTGAGATCTTGAACGACGCCCTCAAAGCTCAGACTGACAATCAACTCAAGAAGTCCCTCTTTGAACAACGAAGGCGGTTGATTCAAGCTATTGATGAGTATGAAGGAGATGACCCTCTGCAGCCGTGGCTTGAGTGTATCAAGTGGGTCCAGGAAGCATTTCCTCCTGGTGGAGATTTCTCGGGACTGGTTGTCATATATGAGCAATGTGTGCGGACATTTTGGCATTCAGACCGCTACAACGACGATCTCCGCTATCTCAAAGTCTGGTTGGAATATGCTGAAAATTGTGCAGATGCTGAAGTCATATTCAGTTTTCTAGATGCAAATGAAATTGGAAAGTCACATTCTCAGTTTTATATATCTTATGCTTTGCTCATGGAATCCAAGAACAAAGTGAAAGCTGCGAATGAACTATTAAATCTTGGGATATCAAGGAATGCTCAGCCCATAGAAAAGTTGAATGATGCTTTTAGGAAGTTTATTGCACGCTCAATGAGAAGACCACCAAAAGCACCAGAGGAGGATTCAGCAGAGAAACGCTTTCCCACCCGGAGCTTTGGGACTGTCCTTGCAAACAGGGAAAATCGTATGCAGACAAGTGAGAGCTCTGGTTTGTTGAAGAAGAATGTGAAGCTAGATAGGGGTCGTGTTCCACTCAGCATTTATAAAGAAGATTCAAATTCCATTGATCAATCAGAAGTGCCAAAGAGAGAATTGAATTCTTGGCACACCCTTGGACCTAGAGCAGAGAGAAATAAAGAAAATACTGCAATCCCTTCCAAGTGGACATCATACAAGATTCGACAAAGACCCGTGCCTAGAGTCGGAGGAGCAACTGCTACTAGTGCCCACATTGAGATTTTCGTCGATCAAGAATGTGAAGAAACACATAAAATGAATGCTGAAGGTGCCAAGTCTTCAACCGTGCAGCCTAGACAGGGTGAAGAGCAAGAGCTGAAGAAGGAAACAGAGTTGTTGAGACGGGAACCATTACGCAATTTCCCTCTAAATAGTCTTCCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009987 GO:0010369 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

389

Amino Acids

44.81

Weight (kDa)

6.34

Isoelectric Point (pI)

56.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 68 - 190 1.2e-42 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33560 AT2G33560
fragaria_vesca FvH4_5g26460 FvH4_5g26460
malus_domestica MD00G1178400.v1.1
prunus_persica Prupe.5G002900_v2.0.a1
pyrus_communis pycom06g00200
rosa_chinensis RchiOBHm_Chr7g0217371
rosa_laevigata RLG00000002485
rosa_multiflora Rmu_sc0011824.1_g000009
rosa_roxburghii Rroxscaffold_3G00242280
rosa_rugosa Rorug07G0168500
rosa_samantha Rh7AG308200 Rh7BG298900 Rh7CG327500 Rh7DG307300
rosa_wichuraiana Rw7G026130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 807
AccB1I GGYRCC 1 cut(s) 1049
AciI CCGC 6 cut(s) 5, 133, 214, 352, 373, 391
AclWI GGATC 2 cut(s) 38, 51
AcsI RAATTY 2 cut(s) 820, 859
AcuI CTGAAG 3 cut(s) 459, 1065, 1118
AcyI GRCGYC 2 cut(s) 65, 156
AfiI CCNNNNNNNGG 3 cut(s) 308, 965, 1079
AhdI GACNNNNNGTC 1 cut(s) 329
AhlI ACTAGT 1 cut(s) 983
AjnI CCWGG 2 cut(s) 288, 304
AluBI AGCT 8 cut(s) 97, 167, 227, 542, 696, 747, 775, 1096
AluI AGCT 8 cut(s) 97, 167, 227, 542, 696, 747, 775, 1096
Alw21I GWGCWC 1 cut(s) 749
Alw26I GTCTC 2 cut(s) 71, 1114
AlwI GGATC 2 cut(s) 38, 51
AlwNI CAGNNNCTG 1 cut(s) 437
Ama87I CYCGRG 1 cut(s) 319
AoxI GGCC 2 cut(s) 116, 130
ApeKI GCWGC 3 cut(s) 259, 542, 1069
ApoI RAATTY 2 cut(s) 820, 859
Asp700I GAANNNNTTC 1 cut(s) 297
AspS9I GGNCC 2 cut(s) 286, 878
AsuC2I CCSGG 1 cut(s) 691
AsuHPI GGTGA 1 cut(s) 1094
AvaI CYCGRG 1 cut(s) 319
AvaII GGWCC 2 cut(s) 286, 878
BaeGI GKGCMC 1 cut(s) 991
BamHI GGATCC 1 cut(s) 43
BanI GGYRCC 1 cut(s) 1049
BanII GRGCYC 1 cut(s) 749
BbsI GAAGAC 3 cut(s) 646, 1050, 1148
Bbv12I GWGCWC 1 cut(s) 749
BbvI GCAGC 3 cut(s) 271, 529, 1081
BceAI ACGGC 2 cut(s) 103, 247
BciT130I CCWGG 2 cut(s) 290, 306
BclI TGATCA 1 cut(s) 829
BcnI CCSGG 1 cut(s) 691
BcoDI GTCTC 2 cut(s) 71, 1114
BcuI ACTAGT 1 cut(s) 983
BfaI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
BfmI CTRYAG 1 cut(s) 257
BglII AGATCT 1 cut(s) 144
BisI GCNGC 4 cut(s) 133, 260, 543, 1070
BlpI GCTNAGC 1 cut(s) 582
BlsI GCNGC 4 cut(s) 134, 261, 544, 1071
Bme1390I CCNGG 3 cut(s) 290, 306, 691
Bme18I GGWCC 2 cut(s) 286, 878
BmeRI GACNNNNNGTC 1 cut(s) 329
BmeT110I CYCGRG 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 286, 878
BmiI GGNNCC 4 cut(s) 45, 287, 1051, 1128
BmrFI CCNGG 3 cut(s) 290, 306, 691
BmsI GCATC 3 cut(s) 424, 451, 595
BpiI GAAGAC 3 cut(s) 646, 1050, 1148
Bpu1102I GCTNAGC 1 cut(s) 582
BpuEI CTTGAG 2 cut(s) 170, 290
BpuMI CCSGG 1 cut(s) 691
BsaHI GRCGYC 2 cut(s) 65, 156
BsaJI CCNNGG 2 cut(s) 263, 874
Bsc4I CCNNNNNNNGG 3 cut(s) 308, 965, 1079
Bse1I ACTGG 1 cut(s) 330
Bse3DI GCAATG 1 cut(s) 350
BseBI CCWGG 2 cut(s) 290, 306
BseDI CCNNGG 2 cut(s) 263, 874
BseLI CCNNNNNNNGG 3 cut(s) 308, 965, 1079
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 4 cut(s) 182, 503, 596, 811
BseNI ACTGG 1 cut(s) 330
BseRI GAGGAG 2 cut(s) 674, 984
BseSI GKGCMC 1 cut(s) 991
BseXI GCAGC 3 cut(s) 271, 529, 1081
BsgI GTGCAG 2 cut(s) 450, 1088
BshFI GGCC 2 cut(s) 118, 132
BshNI GGYRCC 1 cut(s) 1049
BsiHKAI GWGCWC 1 cut(s) 749
BsiHKCI CYCGRG 1 cut(s) 319
BsiSI CCGG 1 cut(s) 691
BslFI GGGAC 3 cut(s) 178, 336, 715
BslI CCNNNNNNNGG 3 cut(s) 308, 965, 1079
BsmAI GTCTC 2 cut(s) 71, 1114
BsmBI CGTCTC 2 cut(s) 71, 1114
BsmFI GGGAC 3 cut(s) 178, 336, 715
BsmI GAATGC 3 cut(s) 364, 583, 1045
BsnI GGCC 2 cut(s) 118, 132
BsoBI CYCGRG 1 cut(s) 319
Bsp1286I GDGCHC 2 cut(s) 749, 991
Bsp143I GATC 5 cut(s) 43, 144, 385, 829, 1009
Bsp1720I GCTNAGC 1 cut(s) 582
BspACI CCGC 6 cut(s) 5, 133, 214, 352, 373, 391
BspANI GGCC 2 cut(s) 118, 132
BspCNI CTCAG 4 cut(s) 181, 502, 595, 810
BspLI GGNNCC 4 cut(s) 45, 287, 1051, 1128
BspMAI CTGCAG 1 cut(s) 261
BspPI GGATC 2 cut(s) 38, 51
BspQI GCTCTTC 1 cut(s) 1080
BspT107I GGYRCC 1 cut(s) 1049
BsrDI GCAATG 1 cut(s) 350
BsrI ACTGG 1 cut(s) 330
BssECI CCNNGG 2 cut(s) 263, 874
BssMI GATC 5 cut(s) 43, 144, 385, 829, 1009
BssNI GRCGYC 2 cut(s) 65, 156
BssT1I CCWWGG 1 cut(s) 874
Bst2UI CCWGG 2 cut(s) 290, 306
Bst4CI ACNGT 3 cut(s) 11, 706, 1066
Bst6I CTCTTC 2 cut(s) 119, 1080
BstACI GRCGYC 2 cut(s) 65, 156
BstAPI GCANNNNNTGC 1 cut(s) 349
BstC8I GCNNGC 1 cut(s) 628
BstDEI CTNAG 4 cut(s) 168, 489, 582, 797
BstDSI CCRYGG 1 cut(s) 263
BstKTI GATC 5 cut(s) 46, 147, 388, 832, 1012
BstMAI GTCTC 2 cut(s) 71, 1114
BstMBI GATC 5 cut(s) 43, 144, 385, 829, 1009
BstMWI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 986, 1049
BstNI CCWGG 2 cut(s) 290, 306
BstSCI CCNGG 3 cut(s) 288, 304, 689
BstSFI CTRYAG 1 cut(s) 257
BstSLI GKGCMC 1 cut(s) 991
BstV1I GCAGC 3 cut(s) 271, 529, 1081
BstV2I GAAGAC 3 cut(s) 646, 1050, 1148
BstX2I RGATCY 2 cut(s) 43, 144
BstYI RGATCY 2 cut(s) 43, 144
BsuRI GGCC 2 cut(s) 118, 132
BtgI CCRYGG 1 cut(s) 263
BtsIMutI CAGTG 1 cut(s) 16
Cac8I GCNNGC 1 cut(s) 628
CaiI CAGNNNCTG 1 cut(s) 437
Cfr13I GGNCC 2 cut(s) 286, 878
CseI GACGC 2 cut(s) 54, 164
CviAII CATG 1 cut(s) 517
DdeI CTNAG 4 cut(s) 168, 489, 582, 797
DpnI GATC 5 cut(s) 45, 146, 387, 831, 1011
DpnII GATC 5 cut(s) 43, 144, 385, 829, 1009
DriI GACNNNNNGTC 1 cut(s) 329
Eam1104I CTCTTC 2 cut(s) 119, 1080
Eam1105I GACNNNNNGTC 1 cut(s) 329
EarI CTCTTC 2 cut(s) 119, 1080
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 747
Eco130I CCWWGG 1 cut(s) 874
Eco24I GRGCYC 1 cut(s) 749
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 2 cut(s) 286, 878
Eco53kI GAGCTC 1 cut(s) 747
Eco57I CTGAAG 3 cut(s) 459, 1065, 1118
Eco88I CYCGRG 1 cut(s) 319
EcoICRI GAGCTC 1 cut(s) 747
EcoRI GAATTC 1 cut(s) 859
EcoRII CCWGG 2 cut(s) 288, 304
EcoRV GATATC 1 cut(s) 570
EcoT14I CCWWGG 1 cut(s) 874
EcoT38I GRGCYC 1 cut(s) 749
ErhI CCWWGG 1 cut(s) 874
Esp3I CGTCTC 2 cut(s) 71, 1114
FaeI CATG 1 cut(s) 520
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 3 cut(s) 178, 336, 715
FatI CATG 1 cut(s) 516
FbaI TGATCA 1 cut(s) 829
Fnu4HI GCNGC 4 cut(s) 133, 260, 543, 1070
FriOI GRGCYC 1 cut(s) 749
Fsp4HI GCNGC 4 cut(s) 133, 260, 543, 1070
FspBI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
GluI GCNGC 4 cut(s) 133, 260, 543, 1070
HaeIII GGCC 2 cut(s) 118, 132
HapII CCGG 1 cut(s) 691
HgaI GACGC 2 cut(s) 54, 164
Hin1I GRCGYC 2 cut(s) 65, 156
Hin1II CATG 1 cut(s) 520
HinfI GANTC 8 cut(s) 50, 70, 220, 521, 665, 815, 940, 963
HpaII CCGG 1 cut(s) 691
HphI GGTGA 1 cut(s) 1094
Hpy166II GTNNAC 1 cut(s) 927
Hpy188I TCNGA 5 cut(s) 55, 171, 370, 838, 968
Hpy188III TCNNGA 7 cut(s) 47, 74, 148, 187, 321, 458, 1013
Hpy8I GTNNAC 1 cut(s) 927
Hpy99I CGWCG 4 cut(s) 70, 158, 386, 1010
HpyAV CCTTC 5 cut(s) 204, 236, 927, 1040, 1096
HpyCH4III ACNGT 3 cut(s) 11, 706, 1066
HpyCH4IV ACGT 1 cut(s) 111
HpyCH4V TGCA 8 cut(s) 259, 431, 464, 626, 713, 733, 911, 1069
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 986, 1049
HpyF3I CTNAG 4 cut(s) 168, 489, 582, 797
HpySE526I ACGT 1 cut(s) 111
Hsp92I GRCGYC 2 cut(s) 65, 156
Hsp92II CATG 1 cut(s) 520
Ksp22I TGATCA 1 cut(s) 829
Kzo9I GATC 5 cut(s) 43, 144, 385, 829, 1009
LguI GCTCTTC 1 cut(s) 1080
LmnI GCTCC 3 cut(s) 94, 693, 971
Lsp1109I GCAGC 3 cut(s) 271, 529, 1081
LweI GCATC 3 cut(s) 424, 451, 595
MaeI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
MaeII ACGT 1 cut(s) 111
MaeIII GTNAC 1 cut(s) 480
MalI GATC 5 cut(s) 45, 146, 387, 831, 1011
MboI GATC 5 cut(s) 43, 144, 385, 829, 1009
MflI RGATCY 2 cut(s) 43, 144
MhlI GDGCHC 2 cut(s) 749, 991
MluCI AATT 7 cut(s) 15, 424, 471, 820, 854, 859, 1138
MlyI GAGTC 1 cut(s) 972
MmeI TCCRAC 2 cut(s) 390, 946
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 1 cut(s) 557
MspI CCGG 1 cut(s) 691
MspR9I CCNGG 3 cut(s) 290, 306, 691
Mva1269I GAATGC 3 cut(s) 364, 583, 1045
MvaI CCWGG 2 cut(s) 290, 306
MwoI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 986, 1049
NciI CCSGG 1 cut(s) 691
NdeII GATC 5 cut(s) 43, 144, 385, 829, 1009
NlaIII CATG 1 cut(s) 520
NlaIV GGNNCC 4 cut(s) 45, 287, 1051, 1128
NmuCI GTSAC 1 cut(s) 480
PciSI GCTCTTC 1 cut(s) 1080
PctI GAATGC 3 cut(s) 364, 583, 1045
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 7 cut(s) 50, 70, 220, 521, 665, 815, 940
PfoI TCCNGGA 1 cut(s) 288
PkrI GCNGC 4 cut(s) 134, 261, 544, 1071
PleI GAGTC 1 cut(s) 971
PpsI GAGTC 1 cut(s) 971
PsiI TTATAA 1 cut(s) 807
Psp124BI GAGCTC 1 cut(s) 749
Psp6I CCWGG 2 cut(s) 288, 304
PspGI CCWGG 2 cut(s) 288, 304
PspN4I GGNNCC 4 cut(s) 45, 287, 1051, 1128
PspPI GGNCC 2 cut(s) 286, 878
PstI CTGCAG 1 cut(s) 261
PstNI CAGNNNCTG 1 cut(s) 437
PsuI RGATCY 2 cut(s) 43, 144
SacI GAGCTC 1 cut(s) 749
SapI GCTCTTC 1 cut(s) 1080
SaqAI TTAA 1 cut(s) 557
SatI GCNGC 4 cut(s) 133, 260, 543, 1070
Sau3AI GATC 5 cut(s) 43, 144, 385, 829, 1009
Sau96I GGNCC 2 cut(s) 286, 878
SchI GAGTC 1 cut(s) 972
ScrFI CCNGG 3 cut(s) 290, 306, 691
SduI GDGCHC 2 cut(s) 749, 991
SfaNI GCATC 3 cut(s) 424, 451, 595
SfcI CTRYAG 1 cut(s) 257
SinI GGWCC 2 cut(s) 286, 878
SmlI CTYRAG 2 cut(s) 185, 269
SmoI CTYRAG 2 cut(s) 185, 269
SpeI ACTAGT 1 cut(s) 983
Sse9I AATT 7 cut(s) 15, 424, 471, 820, 854, 859, 1138
SsiI CCGC 6 cut(s) 5, 133, 214, 352, 373, 391
SspMI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
SstI GAGCTC 1 cut(s) 749
StyD4I CCNGG 3 cut(s) 288, 304, 689
StyI CCWWGG 1 cut(s) 874
TaaI ACNGT 3 cut(s) 11, 706, 1066
TaiI ACGT 1 cut(s) 114
TaqI TCGA 3 cut(s) 68, 943, 1008
TasI AATT 7 cut(s) 15, 424, 471, 820, 854, 859, 1138
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 7 cut(s) 50, 70, 220, 521, 665, 815, 940
Tru1I TTAA 1 cut(s) 557
Tru9I TTAA 1 cut(s) 557
TscAI CASTG 1 cut(s) 16
TseFI GTSAC 1 cut(s) 480
TseI GCWGC 3 cut(s) 259, 542, 1069
Tsp45I GTSAC 1 cut(s) 480
TspDTI ATGAA 4 cut(s) 255, 483, 564, 1052
TspRI CASTG 1 cut(s) 16
VpaK11BI GGWCC 2 cut(s) 286, 878
XapI RAATTY 2 cut(s) 820, 859
XbaI TCTAGA 1 cut(s) 457
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 6 cut(s) 458, 776, 882, 960, 984, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.