Rw7G026130

Mitotic spindle checkpoint protein BUBR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014113
Physical Location & Seq
Reverse (-)
33093798 .. 33097747
3950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G026130.1

Sequence Viewer

Length: 1161 bp
ATGGCGGAAAGTGGAATTGAAATAGAGAAAATAGAGATGTTGGATCCAGAGTCAGAGTTCCTGGCGTCGAATAGGGAGTCAGGGAACGAGTGGGAGCTGTTCAAGGAGAACGTGCGGCCATTGAAGAGAGGCCGCAAAGTTGAAATCTTAAACGACGCTCTCAAAGCTCAAACTGACAACCAACTCAAGAAGTCCCTCTTTGAACAACGAAGGCGGTTGATTCAAGCAATTGACGAGTATGAAGGAGATGACCCTCTGCAGCCTTGGCTCGAGTGTATCAAGTGGGTTCAGGAAGCATTTCCCCCTGGTGGGGATTTCTCGGGACTGGTTGTGATATATGAGCAATGTGTGCGTACATTTTGGCATTCAGACCGCTACAAGGATGATCTTCGTTATCTCAAAGTCTGGTTGGAATATGCTGAAAATTGTGCGGATGCTGAAGTCATATTCAGTTTTCTAGATGCAAATGAAATTGGAAAGACACATTCTCAGTATTACATATCTTATGCTTTGTTGATGGAATCCAAGAACAAAGTGAAAGCTGCCAATGAAATATTAAATCTTGGGATATCAAGGAATGCTCAGCCCACAGAAAAATTGAATGATGCATTTAGGAAATTTCTTGCACGCTCAATGAGAAGACCACCAAAAGCTCCAGAGGAGGATTCAGCAGAGAAACGCTTACCCAACCGAAGCTTTGGAACTGTCCTTGCCAAGGGAGAAAATCGTCTGCAGACAAGTGAGAGCTCTGGTTTGTTGAAGAAGAACGTGAAGCTAGATAGGGGTCGTGTTCCACTTAGCATTTATAAAGAAGAATCCCTTGACCAATCAGAAGTATCAAAGAGAGACGTGAATTCTTGGCACACCCTTGGACCTAGAGCAGAGAGAAATAAAGAAAATAGTGCGATCCCTTCCAAGTGGACATCATATAAGGTTCGTCAAAGACCTGTGCCTAGAATTGGAGGAGCAGCTGCTACTAGTGCCTGCATTGAGATTTTTGTCGATGAAGAATGTGAAGAAACACAGAGAATGAATGCTGAGGGTGCCAAATCTTCAACCTTGCAGCCTAGACAGGGTGAAGAGCAAGAGCTCAAGAAGGAAACAGAGTTGTTGAGACGGCAACCGTTACGCAATTTCCCTCTAAATAGTCTTCCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000075 GO:0000228 GO:0000278 GO:0000280 GO:0000775 GO:0000776 GO:0000777 GO:0000778 GO:0000779 GO:0000780 GO:0000793 GO:0000794 GO:0000819 GO:0003674 GO:0003824 GO:0004672 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005828 GO:0005856 GO:0005874 GO:0005876 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0007062 GO:0007088 GO:0007093 GO:0007094 GO:0007135 GO:0007346 GO:0008150 GO:0008152 GO:0009987 GO:0010369 GO:0010564 GO:0010639 GO:0010948 GO:0010965 GO:0015630 GO:0016043 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0022402 GO:0022414 GO:0030071 GO:0031577 GO:0031974 GO:0031981 GO:0032991 GO:0033043 GO:0033044 GO:0033045 GO:0033046 GO:0033047 GO:0033048 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044430 GO:0044446 GO:0044454 GO:0044464 GO:0045132 GO:0045144 GO:0045786 GO:0045839 GO:0045841 GO:0045930 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051128 GO:0051129 GO:0051177 GO:0051276 GO:0051321 GO:0051726 GO:0051754 GO:0051783 GO:0051784 GO:0051983 GO:0051985 GO:0061983 GO:0065007 GO:0070013 GO:0070192 GO:0070601 GO:0071173 GO:0071174 GO:0071704 GO:0071840 GO:0098687 GO:0098813 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0140013 GO:0140096 GO:1901564 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902099 GO:1902100 GO:1903046 GO:1903047 GO:1905818 GO:1905819 GO:2000816 GO:2001251
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04914 map04110 map04111 map04113 map04114 map04914
Pfam Domains
Protein Families
Mad3_BUB1_I

Protein Analysis

386

Amino Acids

44.48

Weight (kDa)

6.46

Isoelectric Point (pI)

58.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mad3_BUB1_I PF08311 68 - 190 2.1e-43 Mad3/BUB1 homology region 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014113)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G33560 AT2G33560
fragaria_vesca FvH4_5g26460 FvH4_5g26460
malus_domestica MD00G1178400.v1.1
prunus_persica Prupe.5G002900_v2.0.a1
pyrus_communis pycom06g00200
rosa_chinensis RchiOBHm_Chr7g0217371
rosa_laevigata RLG00000002485
rosa_multiflora Rmu_sc0011824.1_g000009
rosa_roxburghii Rroxscaffold_3G00242280
rosa_rugosa Rorug07G0168500
rosa_samantha Rh7AG308200 Rh7BG298900 Rh7CG327500 Rh7DG307300
rosa_wichuraiana Rw7G026130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 807
AccB1I GGYRCC 1 cut(s) 1043
AciI CCGC 6 cut(s) 5, 115, 133, 214, 373, 431
AclWI GGATC 3 cut(s) 38, 51, 901
AcoI YGGCCR 1 cut(s) 116
AcsI RAATTY 2 cut(s) 617, 853
AcuI CTGAAG 1 cut(s) 459
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 1 cut(s) 355
AfiI CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 959, 1073
AgsI TTSAA 9 cut(s) 20, 103, 124, 143, 203, 224, 601, 760, 1056
AhlI ACTAGT 1 cut(s) 977
AjiI CACGTC 1 cut(s) 850
AjnI CCWGG 2 cut(s) 60, 304
AluBI AGCT 9 cut(s) 97, 167, 542, 653, 696, 747, 775, 971, 1090
AluI AGCT 9 cut(s) 97, 167, 542, 653, 696, 747, 775, 971, 1090
Alw21I GWGCWC 2 cut(s) 749, 1092
Alw26I GTCTC 2 cut(s) 840, 1108
AlwI GGATC 3 cut(s) 38, 51, 901
Ama87I CYCGRG 2 cut(s) 269, 319
AoxI GGCC 2 cut(s) 116, 130
ApeKI GCWGC 5 cut(s) 259, 542, 968, 971, 1063
ApoI RAATTY 2 cut(s) 617, 853
ArsI GACNNNNNNTTYG 2 cut(s) 62, 94
Asp700I GAANNNNTTC 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 872
AsuHPI GGTGA 1 cut(s) 1088
AvaI CYCGRG 2 cut(s) 269, 319
AvaII GGWCC 1 cut(s) 872
BamHI GGATCC 1 cut(s) 43
BanI GGYRCC 1 cut(s) 1043
BanII GRGCYC 2 cut(s) 749, 1092
BbsI GAAGAC 2 cut(s) 646, 1142
Bbv12I GWGCWC 2 cut(s) 749, 1092
BbvCI CCTCAGC 1 cut(s) 1038
BbvI GCAGC 5 cut(s) 271, 529, 958, 980, 1075
BccI CCATC 1 cut(s) 511
BceAI ACGGC 1 cut(s) 1133
BciT130I CCWGG 2 cut(s) 62, 306
BcoDI GTCTC 2 cut(s) 840, 1108
BcuI ACTAGT 1 cut(s) 977
BfaI CTAG 6 cut(s) 458, 776, 876, 954, 978, 1068
BfmI CTRYAG 2 cut(s) 257, 731
BisI GCNGC 7 cut(s) 116, 133, 260, 543, 969, 972, 1064
BlpI GCTNAGC 1 cut(s) 582
BlsI GCNGC 7 cut(s) 117, 134, 261, 544, 970, 973, 1065
Bme1390I CCNGG 2 cut(s) 62, 306
Bme18I GGWCC 1 cut(s) 872
BmeT110I CYCGRG 2 cut(s) 269, 319
BmgBI CACGTC 1 cut(s) 850
BmgT120I GGNCC 1 cut(s) 872
BmiI GGNNCC 2 cut(s) 45, 1045
BmrFI CCNGG 2 cut(s) 62, 306
BmsI GCATC 3 cut(s) 424, 451, 595
BpiI GAAGAC 2 cut(s) 646, 1142
BpmI CTGGAG 1 cut(s) 639
Bpu10I CCTNAGC 1 cut(s) 1038
Bpu1102I GCTNAGC 1 cut(s) 582
BpuEI CTTGAG 2 cut(s) 170, 1076
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 4 cut(s) 263, 304, 714, 868
Bsc4I CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 959, 1073
Bse1I ACTGG 1 cut(s) 330
Bse3DI GCAATG 1 cut(s) 350
BseBI CCWGG 2 cut(s) 62, 306
BseDI CCNNGG 4 cut(s) 263, 304, 714, 868
BseGI GGATG 2 cut(s) 388, 439
BseLI CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 959, 1073
BseMI GCAATG 1 cut(s) 350
BseMII CTCAG 3 cut(s) 503, 596, 1029
BseNI ACTGG 1 cut(s) 330
BseRI GAGGAG 2 cut(s) 674, 978
BseXI GCAGC 5 cut(s) 271, 529, 958, 980, 1075
BshFI GGCC 2 cut(s) 118, 132
BshNI GGYRCC 1 cut(s) 1043
BsiHKAI GWGCWC 2 cut(s) 749, 1092
BsiHKCI CYCGRG 2 cut(s) 269, 319
BslFI GGGAC 2 cut(s) 178, 336
BslI CCNNNNNNNGG 7 cut(s) 308, 309, 310, 379, 715, 959, 1073
BsmAI GTCTC 2 cut(s) 840, 1108
BsmBI CGTCTC 2 cut(s) 840, 1108
BsmFI GGGAC 2 cut(s) 178, 336
BsmI GAATGC 3 cut(s) 364, 583, 1039
BsnI GGCC 2 cut(s) 118, 132
BsoBI CYCGRG 2 cut(s) 269, 319
Bsp1286I GDGCHC 2 cut(s) 749, 1092
Bsp143I GATC 3 cut(s) 43, 385, 906
Bsp1720I GCTNAGC 1 cut(s) 582
BspACI CCGC 6 cut(s) 5, 115, 133, 214, 373, 431
BspANI GGCC 2 cut(s) 118, 132
BspCNI CTCAG 3 cut(s) 502, 595, 1030
BspLI GGNNCC 2 cut(s) 45, 1045
BspMAI CTGCAG 2 cut(s) 261, 735
BspPI GGATC 3 cut(s) 38, 51, 901
BspQI GCTCTTC 1 cut(s) 1074
BspT107I GGYRCC 1 cut(s) 1043
BsrDI GCAATG 1 cut(s) 350
BsrI ACTGG 1 cut(s) 330
BssECI CCNNGG 4 cut(s) 263, 304, 714, 868
BssMI GATC 3 cut(s) 43, 385, 906
BssNI GRCGYC 1 cut(s) 65
BssT1I CCWWGG 3 cut(s) 263, 714, 868
Bst2UI CCWGG 2 cut(s) 62, 306
Bst4CI ACNGT 2 cut(s) 706, 1125
Bst6I CTCTTC 2 cut(s) 119, 1074
BstACI GRCGYC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 628, 985
BstDEI CTNAG 4 cut(s) 489, 582, 797, 1038
BstENI CCTNNNNNAGG 1 cut(s) 713
BstF5I GGATG 2 cut(s) 388, 439
BstKTI GATC 3 cut(s) 46, 388, 909
BstMAI GTCTC 2 cut(s) 840, 1108
BstMBI GATC 3 cut(s) 43, 385, 906
BstMWI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 980, 1043
BstNI CCWGG 2 cut(s) 62, 306
BstSCI CCNGG 2 cut(s) 60, 304
BstSFI CTRYAG 2 cut(s) 257, 731
BstV1I GCAGC 5 cut(s) 271, 529, 958, 980, 1075
BstV2I GAAGAC 2 cut(s) 646, 1142
BstX2I RGATCY 1 cut(s) 43
BstYI RGATCY 1 cut(s) 43
BsuRI GGCC 2 cut(s) 118, 132
BtrI CACGTC 1 cut(s) 850
BtsCI GGATG 2 cut(s) 388, 439
Cac8I GCNNGC 2 cut(s) 628, 985
Cfr13I GGNCC 1 cut(s) 872
CseI GACGC 2 cut(s) 54, 164
Csp6I GTAC 1 cut(s) 354
CviQI GTAC 1 cut(s) 354
DdeI CTNAG 4 cut(s) 489, 582, 797, 1038
DpnI GATC 3 cut(s) 45, 387, 908
DpnII GATC 3 cut(s) 43, 385, 906
EaeI YGGCCR 1 cut(s) 116
Eam1104I CTCTTC 2 cut(s) 119, 1074
EarI CTCTTC 2 cut(s) 119, 1074
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 2 cut(s) 747, 1090
Eco130I CCWWGG 3 cut(s) 263, 714, 868
Eco24I GRGCYC 2 cut(s) 749, 1092
Eco32I GATATC 1 cut(s) 570
Eco47I GGWCC 1 cut(s) 872
Eco53kI GAGCTC 2 cut(s) 747, 1090
Eco57I CTGAAG 1 cut(s) 459
Eco88I CYCGRG 2 cut(s) 269, 319
EcoICRI GAGCTC 2 cut(s) 747, 1090
EcoNI CCTNNNNNAGG 1 cut(s) 713
EcoRI GAATTC 1 cut(s) 853
EcoRII CCWGG 2 cut(s) 60, 304
EcoRV GATATC 1 cut(s) 570
EcoT14I CCWWGG 3 cut(s) 263, 714, 868
EcoT22I ATGCAT 1 cut(s) 610
EcoT38I GRGCYC 2 cut(s) 749, 1092
ErhI CCWWGG 3 cut(s) 263, 714, 868
Esp3I CGTCTC 2 cut(s) 840, 1108
FalI AAGNNNNNCTT 4 cut(s) 182, 214, 804, 836
FaqI GGGAC 2 cut(s) 178, 336
Fnu4HI GCNGC 7 cut(s) 116, 133, 260, 543, 969, 972, 1064
FokI GGATG 2 cut(s) 395, 446
FriOI GRGCYC 2 cut(s) 749, 1092
Fsp4HI GCNGC 7 cut(s) 116, 133, 260, 543, 969, 972, 1064
FspBI CTAG 6 cut(s) 458, 776, 876, 954, 978, 1068
GluI GCNGC 7 cut(s) 116, 133, 260, 543, 969, 972, 1064
GsuI CTGGAG 1 cut(s) 639
HaeIII GGCC 2 cut(s) 118, 132
HgaI GACGC 2 cut(s) 54, 164
Hin1I GRCGYC 1 cut(s) 65
HindIII AAGCTT 1 cut(s) 694
HinfI GANTC 6 cut(s) 50, 77, 220, 521, 665, 815
HphI GGTGA 1 cut(s) 1088
Hpy166II GTNNAC 1 cut(s) 921
Hpy188I TCNGA 3 cut(s) 55, 370, 832
Hpy188III TCNNGA 7 cut(s) 47, 187, 290, 321, 458, 656, 1093
Hpy8I GTNNAC 1 cut(s) 921
Hpy99I CGWCG 2 cut(s) 70, 158
HpyAV CCTTC 4 cut(s) 204, 236, 921, 1090
HpyCH4III ACNGT 2 cut(s) 706, 1125
HpyCH4IV ACGT 3 cut(s) 111, 768, 849
HpyCH4V TGCA 7 cut(s) 259, 464, 608, 626, 733, 987, 1063
HpyF10VI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 980, 1043
HpyF3I CTNAG 4 cut(s) 489, 582, 797, 1038
HpySE526I ACGT 3 cut(s) 111, 768, 849
Hsp92I GRCGYC 1 cut(s) 65
Kzo9I GATC 3 cut(s) 43, 385, 906
LguI GCTCTTC 1 cut(s) 1074
LmnI GCTCC 3 cut(s) 94, 658, 965
Lsp1109I GCAGC 5 cut(s) 271, 529, 958, 980, 1075
LweI GCATC 3 cut(s) 424, 451, 595
MaeI CTAG 6 cut(s) 458, 776, 876, 954, 978, 1068
MaeII ACGT 3 cut(s) 111, 768, 849
MaeIII GTNAC 1 cut(s) 1125
MalI GATC 3 cut(s) 45, 387, 908
MboI GATC 3 cut(s) 43, 385, 906
MfeI CAATTG 1 cut(s) 228
MflI RGATCY 1 cut(s) 43
MhlI GDGCHC 2 cut(s) 749, 1092
MluCI AATT 9 cut(s) 15, 228, 424, 471, 596, 617, 853, 957, 1132
MlyI GAGTC 2 cut(s) 59, 86
MmeI TCCRAC 2 cut(s) 21, 390
MnlI CCTC 8 cut(s) 122, 206, 264, 652, 655, 956, 1033, 1149
Mph1103I ATGCAT 1 cut(s) 610
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 2 cut(s) 149, 557
MspA1I CMGCKG 1 cut(s) 971
MspR9I CCNGG 2 cut(s) 62, 306
MunI CAATTG 1 cut(s) 228
Mva1269I GAATGC 3 cut(s) 364, 583, 1039
MvaI CCWGG 2 cut(s) 62, 306
MwoI GCNNNNNNNGC 5 cut(s) 164, 265, 349, 980, 1043
NdeII GATC 3 cut(s) 43, 385, 906
NlaIV GGNNCC 2 cut(s) 45, 1045
NsiI ATGCAT 1 cut(s) 610
PaeR7I CTCGAG 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 1074
PctI GAATGC 3 cut(s) 364, 583, 1039
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 4 cut(s) 220, 521, 665, 815
PkrI GCNGC 7 cut(s) 117, 134, 261, 544, 970, 973, 1065
PleI GAGTC 2 cut(s) 58, 85
PpsI GAGTC 2 cut(s) 58, 85
PsiI TTATAA 1 cut(s) 807
Psp124BI GAGCTC 2 cut(s) 749, 1092
Psp6I CCWGG 2 cut(s) 60, 304
PspGI CCWGG 2 cut(s) 60, 304
PspN4I GGNNCC 2 cut(s) 45, 1045
PspPI GGNCC 1 cut(s) 872
PspXI VCTCGAGB 1 cut(s) 269
PstI CTGCAG 2 cut(s) 261, 735
PsuI RGATCY 1 cut(s) 43
PvuII CAGCTG 1 cut(s) 971
RsaI GTAC 1 cut(s) 355
RsaNI GTAC 1 cut(s) 354
SacI GAGCTC 2 cut(s) 749, 1092
SapI GCTCTTC 1 cut(s) 1074
SaqAI TTAA 2 cut(s) 149, 557
SatI GCNGC 7 cut(s) 116, 133, 260, 543, 969, 972, 1064
Sau3AI GATC 3 cut(s) 43, 385, 906
Sau96I GGNCC 1 cut(s) 872
SchI GAGTC 2 cut(s) 59, 86
ScrFI CCNGG 2 cut(s) 62, 306
SduI GDGCHC 2 cut(s) 749, 1092
SfaNI GCATC 3 cut(s) 424, 451, 595
SfcI CTRYAG 2 cut(s) 257, 731
Sfr274I CTCGAG 1 cut(s) 269
SinI GGWCC 1 cut(s) 872
SlaI CTCGAG 1 cut(s) 269
SmlI CTYRAG 3 cut(s) 185, 269, 1091
SmoI CTYRAG 3 cut(s) 185, 269, 1091
SpeI ACTAGT 1 cut(s) 977
Sse9I AATT 9 cut(s) 15, 228, 424, 471, 596, 617, 853, 957, 1132
SsiI CCGC 6 cut(s) 5, 115, 133, 214, 373, 431
SspI AATATT 1 cut(s) 555
SspMI CTAG 6 cut(s) 458, 776, 876, 954, 978, 1068
SstI GAGCTC 2 cut(s) 749, 1092
StyD4I CCNGG 2 cut(s) 60, 304
StyI CCWWGG 3 cut(s) 263, 714, 868
TaaI ACNGT 2 cut(s) 706, 1125
TaiI ACGT 3 cut(s) 114, 771, 852
TaqI TCGA 3 cut(s) 68, 270, 1002
TasI AATT 9 cut(s) 15, 228, 424, 471, 596, 617, 853, 957, 1132
TauI GCSGC 2 cut(s) 118, 135
TfiI GAWTC 4 cut(s) 220, 521, 665, 815
Tru1I TTAA 2 cut(s) 149, 557
Tru9I TTAA 2 cut(s) 149, 557
TseI GCWGC 5 cut(s) 259, 542, 968, 971, 1063
TspDTI ATGAA 5 cut(s) 255, 483, 564, 1020, 1046
VpaK11BI GGWCC 1 cut(s) 872
XagI CCTNNNNNAGG 1 cut(s) 713
XapI RAATTY 2 cut(s) 617, 853
XbaI TCTAGA 1 cut(s) 457
XhoI CTCGAG 1 cut(s) 269
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 6 cut(s) 458, 776, 876, 954, 978, 1068
Zsp2I ATGCAT 1 cut(s) 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.