Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0009031

Physical Location & Seq

Genomic Coordinates Reverse (-)

14751541 .. 14753319

Gene Structure 1779 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G35000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1137 bp

ATGGCGATCCTCGACACAAAGTCCAGCCGTTGGATCCCACACAACCTTCTCTTTCTTCTCCTCTTGCTCTTGCTCCAGTCAGTACCGTATGGATTCGGTCAGACTCAGACCACACCACCCGGAACAACAAAAACGAAACCTAACGATCCAGTTGTTGTTGTAATCACGGTTTTGTTTTTAGTGATCTTTTTCATGGTCTTCGGTTCCATCTTCTGTCGTCGAAGCAACGCACAGTTTTCACGTTCTTCCATTTTCCGATCCACCGACGCAGACGCCGAGTCTCAGGTCGTCAGGATCAGGAGGTTGACGGCGCGTGGGCTCGACGCGGAGGCTATAGAAACTTTCCCGACGTTTCTTTACTCGGAGGTGAAGGCGGTGAGGATCGGGAAAGGTGGTGTGGAGTGTGCGGTTTGTCTCTGTGAATTCGAAGACGACGAAACGCTGCGTTTGATGCCTCCTTGTTGCCACGTGTTTCACGCCGATTGTGTTGACGTCTGGCTCTCAGAACACTCCACGTGTCCTCTCTGTAGAGCGGATCTTGTCCTAAACCAACAAGGTGACGACGACGACAGCACTGAAAGCTACTCGGGTACGGATCCAGGTACGATTTCGTCGAGTACGGATCCTGAGAGAGGAATGGTTTTGGAATCTTCGGACGCGCATTTGCTGGATGCTGTGACGTGGAGCAATAGTAACATAACACCTCGGTCAAAGTCAACGGGGTTATCCAGCTGGCAAATAACCGGAATTTTGTTCCCGAGATCGCACTCGACCGGACATTCGTTAATTCAACCGGCTGGGAATCTAGACCGGTTTACGTTGAGGTTACCGGATGATGTACGACGGCAACTTATGAAGACATCGAGGACGATGGGACACGTGGCGTTACTACCTCAGGCGAGGAGTTCACGGAGCGGTTATAGAAGCGGTAGCGTCGGGAGCGAGAGAAGCGCTTTCCCGTACGGACGTAAGAGTAATAATAACAATCGACGGCTTCATTCGCTGTCTTTTTCATTCTCTTTCCGGAGTGGTTCCGTACGGTCTACTTTCTCCGGAGATGCGCCGAAGAATCTTCCGACATCGATTGAAGCTGGTGAACGGTCTTTCGAACGGCTCCGACCAGATGAACGAGTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0006950 GO:0006952 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009605 GO:0009607 GO:0009620 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0042221 GO:0042493 GO:0043161 GO:0043170 GO:0043207 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0098542 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901698 GO:1901700

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

378

Amino Acids

41.88

Weight (kDa)

8.09

Isoelectric Point (pI)

56.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	134 - 177	2.1e-13	Ring finger domain
zf-RING_11	PF17123	134 - 163	8.3e-09	RING-like zinc finger
zf-C3HC4_2	PF13923	134 - 176	8.6e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	137 - 177	3.3e-07	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009031)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G35330 AT2G34990 AT2G34990 AT2G35000
fragaria_vesca	FvH4_5g36230
malus_domestica	MD08G1190600.v1.1 MD15G1377900.v1.1
prunus_persica	Prupe.1G524600_v2.0.a1
pyrus_communis	pycom08g16380 pycom15g33890
rosa_chinensis	RchiOBHm_Chr7g0238061
rosa_laevigata	RLG00000000944
rosa_multiflora	Rmu_co8161516.1_g000001 Rmu_sc0002586.1_g000017 Rmu_sc0024940.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223410
rosa_rugosa	Rorug07G0310300.1
rosa_samantha	Rh7AG465500 Rh7BG436500 Rh7CG483800 Rh7DG452200
rosa_wichuraiana	Rw7G038560

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	495
AccB7I	CCANNNNNTGG	1 cut(s)	30
AccBSI	CCGCTC	2 cut(s)	533, 915
AccI	GTMKAC	1 cut(s)	1043
AccII	CGCG	3 cut(s)	313, 326, 659
AccIII	TCCGGA	2 cut(s)	1023, 1052
AciI	CCGC	6 cut(s)	326, 374, 407, 533, 915, 927
AcsI	RAATTY	2 cut(s)	422, 747
AcvI	CACGTG	3 cut(s)	469, 516, 880
AcyI	GRCGYC	2 cut(s)	273, 492
AfaI	GTAC	7 cut(s)	84, 592, 604, 619, 840, 962, 1038
AfeI	AGCGCT	1 cut(s)	952
AfiI	CCNNNNNNNGG	2 cut(s)	30, 632
AflIII	ACRYGT	3 cut(s)	468, 515, 877
AgeI	ACCGGT	1 cut(s)	810
AgsI	TTSAA	2 cut(s)	791, 1088
AhdI	GACNNNNNGTC	2 cut(s)	19, 277
AjiI	CACGTC	1 cut(s)	681
AjnI	CCWGG	1 cut(s)	598
AluBI	AGCT	3 cut(s)	582, 732, 1091
AluI	AGCT	3 cut(s)	582, 732, 1091
Alw26I	GTCTC	2 cut(s)	285, 419
Ama87I	CYCGRG	2 cut(s)	586, 757
Aor13HI	TCCGGA	2 cut(s)	1023, 1052
Aor51HI	AGCGCT	1 cut(s)	952
ApeKI	GCWGC	1 cut(s)	442
ApoI	RAATTY	2 cut(s)	422, 747
ArsI	GACNNNNNNTTYG	2 cut(s)	763, 795
AsiGI	ACCGGT	1 cut(s)	810
AspLEI	GCGC	4 cut(s)	313, 661, 953, 1063
AsuC2I	CCSGG	1 cut(s)	120
AsuHPI	GGTGA	4 cut(s)	379, 388, 569, 1106
AsuII	TTCGAA	2 cut(s)	426, 1107
AvaI	CYCGRG	2 cut(s)	586, 757
AxyI	CCTNAGG	1 cut(s)	894
BamHI	GGATCC	3 cut(s)	33, 595, 622
BanII	GRGCYC	1 cut(s)	321
BbrPI	CACGTG	3 cut(s)	469, 516, 880
BbsI	GAAGAC	3 cut(s)	190, 435, 863
BbvI	GCAGC	1 cut(s)	429
BccI	CCATC	2 cut(s)	215, 865
BceAI	ACGGC	5 cut(s)	12, 324, 860, 1007, 1127
BciT130I	CCWGG	1 cut(s)	600
BcnI	CCSGG	1 cut(s)	120
BcoDI	GTCTC	2 cut(s)	285, 419
BfaI	CTAG	1 cut(s)	806
BfmI	CTRYAG	2 cut(s)	333, 526
BfoI	RGCGCY	1 cut(s)	954
BisI	GCNGC	1 cut(s)	443
BlsI	GCNGC	1 cut(s)	444
Bme1390I	CCNGG	2 cut(s)	120, 600
BmeRI	GACNNNNNGTC	2 cut(s)	19, 277
BmeT110I	CYCGRG	2 cut(s)	586, 757
BmgBI	CACGTC	1 cut(s)	681
BmiI	GGNNCC	6 cut(s)	35, 205, 597, 624, 1033, 1115
BmrFI	CCNGG	2 cut(s)	120, 600
BmsI	GCATC	3 cut(s)	441, 661, 1048
BpiI	GAAGAC	3 cut(s)	190, 435, 863
BpmI	CTGGAG	1 cut(s)	59
Bpu14I	TTCGAA	2 cut(s)	426, 1107
BpuMI	CCSGG	1 cut(s)	120
Bsa29I	ATCGAT	1 cut(s)	1082
BsaAI	YACGTR	3 cut(s)	469, 516, 880
BsaHI	GRCGYC	2 cut(s)	273, 492
BsaJI	CCNNGG	1 cut(s)	704
BsaWI	WCCGGW	6 cut(s)	743, 773, 810, 829, 1023, 1052
BsaXI	ACNNNNNCTCC	2 cut(s)	676, 706
Bsc4I	CCNNNNNNNGG	2 cut(s)	30, 632
Bse118I	RCCGGY	2 cut(s)	793, 810
Bse1I	ACTGG	2 cut(s)	76, 149
Bse21I	CCTNAGG	1 cut(s)	894
BseAI	TCCGGA	2 cut(s)	1023, 1052
BseBI	CCWGG	1 cut(s)	600
BseCI	ATCGAT	1 cut(s)	1082
BseDI	CCNNGG	1 cut(s)	704
BseGI	GGATG	2 cut(s)	676, 838
BseLI	CCNNNNNNNGG	2 cut(s)	30, 632
BseMII	CTCAG	5 cut(s)	119, 296, 516, 618, 908
BseNI	ACTGG	2 cut(s)	76, 149
BseRI	GAGGAG	2 cut(s)	50, 916
BseXI	GCAGC	1 cut(s)	429
BseYI	CCCAGC	1 cut(s)	797
Bsh1236I	CGCG	3 cut(s)	313, 326, 659
Bsh1285I	CGRYCG	1 cut(s)	774
BshTI	ACCGGT	1 cut(s)	810
BshVI	ATCGAT	1 cut(s)	1082
BsiEI	CGRYCG	1 cut(s)	774
BsiHKCI	CYCGRG	2 cut(s)	586, 757
BsiSI	CCGG	8 cut(s)	120, 744, 774, 794, 811, 830, 1024, 1053
BsiWI	CGTACG	2 cut(s)	960, 1036
BslFI	GGGAC	1 cut(s)	888
BslI	CCNNNNNNNGG	2 cut(s)	30, 632
BsmAI	GTCTC	2 cut(s)	285, 419
BsmFI	GGGAC	1 cut(s)	888
BsoBI	CYCGRG	2 cut(s)	586, 757
Bsp119I	TTCGAA	2 cut(s)	426, 1107
Bsp1286I	GDGCHC	1 cut(s)	321
Bsp13I	TCCGGA	2 cut(s)	1023, 1052
BspACI	CCGC	6 cut(s)	326, 374, 407, 533, 915, 927
BspCNI	CTCAG	5 cut(s)	118, 295, 515, 619, 907
BspDI	ATCGAT	1 cut(s)	1082
BspEI	TCCGGA	2 cut(s)	1023, 1052
BspFNI	CGCG	3 cut(s)	313, 326, 659
BspLI	GGNNCC	6 cut(s)	35, 205, 597, 624, 1033, 1115
BspT104I	TTCGAA	2 cut(s)	426, 1107
BsrBI	CCGCTC	2 cut(s)	533, 915
BsrFI	RCCGGY	2 cut(s)	793, 810
BsrI	ACTGG	2 cut(s)	76, 149
BssAI	RCCGGY	2 cut(s)	793, 810
BssECI	CCNNGG	1 cut(s)	704
BssNI	GRCGYC	2 cut(s)	273, 492
Bst2UI	CCWGG	1 cut(s)	600
Bst4CI	ACNGT	5 cut(s)	87, 169, 234, 1041, 1101
BstACI	GRCGYC	2 cut(s)	273, 492
BstBAI	YACGTR	3 cut(s)	469, 516, 880
BstBI	TTCGAA	2 cut(s)	426, 1107
BstC8I	GCNNGC	1 cut(s)	734
BstDEI	CTNAG	5 cut(s)	105, 282, 502, 627, 894
BstEII	GGTNACC	1 cut(s)	825
BstENI	CCTNNNNNAGG	1 cut(s)	630
BstF5I	GGATG	2 cut(s)	676, 838
BstFNI	CGCG	3 cut(s)	313, 326, 659
BstH2I	RGCGCY	1 cut(s)	954
BstHHI	GCGC	4 cut(s)	313, 661, 953, 1063
BstMAI	GTCTC	2 cut(s)	285, 419
BstMCI	CGRYCG	1 cut(s)	774
BstMWI	GCNNNNNNNGC	5 cut(s)	451, 579, 939, 948, 1000
BstNI	CCWGG	1 cut(s)	600
BstPI	GGTNACC	1 cut(s)	825
BstSCI	CCNGG	2 cut(s)	118, 598
BstSFI	CTRYAG	2 cut(s)	333, 526
BstUI	CGCG	3 cut(s)	313, 326, 659
BstV1I	GCAGC	1 cut(s)	429
BstV2I	GAAGAC	3 cut(s)	190, 435, 863
BstX2I	RGATCY	4 cut(s)	33, 535, 595, 622
BstYI	RGATCY	4 cut(s)	33, 535, 595, 622
Bsu15I	ATCGAT	1 cut(s)	1082
Bsu36I	CCTNAGG	1 cut(s)	894
BsuTUI	ATCGAT	1 cut(s)	1082
BtrI	CACGTC	1 cut(s)	681
BtsCI	GGATG	2 cut(s)	676, 838
BtsIMutI	CAGTG	1 cut(s)	573
Cac8I	GCNNGC	1 cut(s)	734
CfoI	GCGC	4 cut(s)	313, 661, 953, 1063
Cfr10I	RCCGGY	2 cut(s)	793, 810
ClaI	ATCGAT	1 cut(s)	1082
CseI	GACGC	5 cut(s)	275, 281, 332, 665, 922
Csp6I	GTAC	7 cut(s)	83, 591, 603, 618, 839, 961, 1037
CspAI	ACCGGT	1 cut(s)	810
CviAII	CATG	1 cut(s)	193
CviQI	GTAC	7 cut(s)	83, 591, 603, 618, 839, 961, 1037
DdeI	CTNAG	5 cut(s)	105, 282, 502, 627, 894
DriI	GACNNNNNGTC	2 cut(s)	19, 277
Eam1105I	GACNNNNNGTC	2 cut(s)	19, 277
Eco24I	GRGCYC	1 cut(s)	321
Eco47III	AGCGCT	1 cut(s)	952
Eco72I	CACGTG	3 cut(s)	469, 516, 880
Eco81I	CCTNAGG	1 cut(s)	894
Eco88I	CYCGRG	2 cut(s)	586, 757
Eco91I	GGTNACC	1 cut(s)	825
EcoNI	CCTNNNNNAGG	1 cut(s)	630
EcoO65I	GGTNACC	1 cut(s)	825
EcoRI	GAATTC	1 cut(s)	422
EcoRII	CCWGG	1 cut(s)	598
EcoT38I	GRGCYC	1 cut(s)	321
FaeI	CATG	1 cut(s)	196
FaiI	YATR	6 cut(s)	90, 194, 335, 698, 854, 921
FaqI	GGGAC	1 cut(s)	888
FatI	CATG	1 cut(s)	192
FblI	GTMKAC	1 cut(s)	1043
Fnu4HI	GCNGC	1 cut(s)	443
FokI	GGATG	2 cut(s)	683, 845
FriOI	GRGCYC	1 cut(s)	321
Fsp4HI	GCNGC	1 cut(s)	443
FspBI	CTAG	1 cut(s)	806
GlaI	GCGC	4 cut(s)	312, 660, 952, 1062
GluI	GCNGC	1 cut(s)	443
GsaI	CCCAGC	1 cut(s)	801
GsuI	CTGGAG	1 cut(s)	59
HaeII	RGCGCY	1 cut(s)	954
HapII	CCGG	8 cut(s)	120, 744, 774, 794, 811, 830, 1024, 1053
HgaI	GACGC	5 cut(s)	275, 281, 332, 665, 922
HhaI	GCGC	4 cut(s)	313, 661, 953, 1063
Hin1I	GRCGYC	2 cut(s)	273, 492
Hin1II	CATG	1 cut(s)	196
Hin6I	GCGC	4 cut(s)	311, 659, 951, 1061
HinP1I	GCGC	4 cut(s)	311, 659, 951, 1061
HincII	GTYRAC	3 cut(s)	306, 490, 717
HindII	GTYRAC	3 cut(s)	306, 490, 717
HinfI	GANTC	6 cut(s)	93, 103, 278, 647, 802, 1069
HpaII	CCGG	8 cut(s)	120, 744, 774, 794, 811, 830, 1024, 1053
HphI	GGTGA	4 cut(s)	379, 388, 569, 1106
Hpy166II	GTNNAC	7 cut(s)	306, 490, 717, 816, 908, 1044, 1097
Hpy188I	TCNGA	8 cut(s)	102, 108, 257, 364, 505, 655, 1077, 1118
Hpy8I	GTNNAC	7 cut(s)	306, 490, 717, 816, 908, 1044, 1097
HpyAV	CCTTC	2 cut(s)	56, 364
HpyCH4III	ACNGT	5 cut(s)	87, 169, 234, 1041, 1101
HpyCH4IV	ACGT	9 cut(s)	241, 350, 468, 492, 515, 680, 818, 879, 967
HpyF10VI	GCNNNNNNNGC	5 cut(s)	451, 579, 939, 948, 1000
HpyF3I	CTNAG	5 cut(s)	105, 282, 502, 627, 894
HpySE526I	ACGT	9 cut(s)	241, 350, 468, 492, 515, 680, 818, 879, 967
Hsp92I	GRCGYC	2 cut(s)	273, 492
Hsp92II	CATG	1 cut(s)	196
HspAI	GCGC	4 cut(s)	311, 659, 951, 1061
Kpn2I	TCCGGA	2 cut(s)	1023, 1052
LmnI	GCTCC	5 cut(s)	78, 684, 912, 939, 1119
Lsp1109I	GCAGC	1 cut(s)	429
LweI	GCATC	3 cut(s)	441, 661, 1048
MaeI	CTAG	1 cut(s)	806
MaeII	ACGT	9 cut(s)	241, 350, 468, 492, 515, 680, 818, 879, 967
MaeIII	GTNAC	5 cut(s)	557, 676, 692, 825, 885
MbiI	CCGCTC	2 cut(s)	533, 915
MboII	GAAGA	9 cut(s)	47, 190, 202, 237, 440, 642, 868, 1064, 1078
MflI	RGATCY	4 cut(s)	33, 535, 595, 622
MhlI	GDGCHC	1 cut(s)	321
MluCI	AATT	3 cut(s)	422, 747, 786
MlyI	GAGTC	2 cut(s)	97, 287
MmeI	TCCRAC	2 cut(s)	11, 1100
MroI	TCCGGA	2 cut(s)	1023, 1052
MseI	TTAA	1 cut(s)	785
MspA1I	CMGCKG	1 cut(s)	732
MspI	CCGG	8 cut(s)	120, 744, 774, 794, 811, 830, 1024, 1053
MspR9I	CCNGG	2 cut(s)	120, 600
MvaI	CCWGG	1 cut(s)	600
MvnI	CGCG	3 cut(s)	313, 326, 659
MwoI	GCNNNNNNNGC	5 cut(s)	451, 579, 939, 948, 1000
NciI	CCSGG	1 cut(s)	120
NlaIII	CATG	1 cut(s)	196
NlaIV	GGNNCC	6 cut(s)	35, 205, 597, 624, 1033, 1115
NmeAIII	GCCGAG	1 cut(s)	301
NmuCI	GTSAC	2 cut(s)	557, 676
NspV	TTCGAA	2 cut(s)	426, 1107
PcsI	WCGNNNNNNNCGW	3 cut(s)	273, 432, 611
PfeI	GAWTC	4 cut(s)	93, 647, 802, 1069
Pfl23II	CGTACG	2 cut(s)	960, 1036
PflMI	CCANNNNNTGG	1 cut(s)	30
PinAI	ACCGGT	1 cut(s)	810
PkrI	GCNGC	1 cut(s)	444
PleI	GAGTC	2 cut(s)	97, 286
PmaCI	CACGTG	3 cut(s)	469, 516, 880
PmlI	CACGTG	3 cut(s)	469, 516, 880
PpsI	GAGTC	2 cut(s)	97, 286
Ppu21I	YACGTR	3 cut(s)	469, 516, 880
Psp6I	CCWGG	1 cut(s)	598
PspCI	CACGTG	3 cut(s)	469, 516, 880
PspEI	GGTNACC	1 cut(s)	825
PspFI	CCCAGC	1 cut(s)	797
PspGI	CCWGG	1 cut(s)	598
PspLI	CGTACG	2 cut(s)	960, 1036
PspN4I	GGNNCC	6 cut(s)	35, 205, 597, 624, 1033, 1115
PsuI	RGATCY	4 cut(s)	33, 535, 595, 622
PvuII	CAGCTG	1 cut(s)	732
RsaI	GTAC	7 cut(s)	84, 592, 604, 619, 840, 962, 1038
RsaNI	GTAC	7 cut(s)	83, 591, 603, 618, 839, 961, 1037
SaqAI	TTAA	1 cut(s)	785
SatI	GCNGC	1 cut(s)	443
SchI	GAGTC	2 cut(s)	97, 287
ScrFI	CCNGG	2 cut(s)	120, 600
SduI	GDGCHC	1 cut(s)	321
SfaNI	GCATC	3 cut(s)	441, 661, 1048
SfcI	CTRYAG	2 cut(s)	333, 526
SfuI	TTCGAA	2 cut(s)	426, 1107
Sse9I	AATT	3 cut(s)	422, 747, 786
SsiI	CCGC	6 cut(s)	326, 374, 407, 533, 915, 927
SspMI	CTAG	1 cut(s)	806
StyD4I	CCNGG	2 cut(s)	118, 598
TaaI	ACNGT	5 cut(s)	87, 169, 234, 1041, 1101
TaiI	ACGT	9 cut(s)	244, 353, 471, 495, 518, 683, 821, 882, 970
TaqII	GACCGA	2 cut(s)	86, 696
TasI	AATT	3 cut(s)	422, 747, 786
TfiI	GAWTC	4 cut(s)	93, 647, 802, 1069
Tru1I	TTAA	1 cut(s)	785
Tru9I	TTAA	1 cut(s)	785
TscAI	CASTG	1 cut(s)	580
TseFI	GTSAC	2 cut(s)	557, 676
TseI	GCWGC	1 cut(s)	442
Tsp45I	GTSAC	2 cut(s)	557, 676
TspDTI	ATGAA	4 cut(s)	181, 869, 986, 1002
TspGWI	ACGGA	5 cut(s)	608, 635, 925, 978, 1024
TspRI	CASTG	1 cut(s)	580
Van91I	CCANNNNNTGG	1 cut(s)	30
XagI	CCTNNNNNAGG	1 cut(s)	630
XapI	RAATTY	2 cut(s)	422, 747
XbaI	TCTAGA	1 cut(s)	805
XmiI	GTMKAC	1 cut(s)	1043
XspI	CTAG	1 cut(s)	806
ZraI	GACGTC	1 cut(s)	493

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.