Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008534

Physical Location & Seq

Genomic Coordinates Reverse (-)

14795715 .. 14803971

Gene Structure 8257 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G35110.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4278 bp

ATGGCGAATTCTCGTCAATATTATCCATCTCAAGATGAATCAATGTCCCCGACTTCTGTGAGATCAAGGGAATGGGAAGGTCCTTCCAGATGGACTGAGTACTTGGGACCTGAAATGGCTGCATCAGTGTCTTCTACTAGGAGCTCTAAGCAAATTGATGGACATGTTGGTGGTTCCACAAAGGCTTTGAACATACAATGGGTAGTTCAAATGATTGAGGTTGCTGACGGTCTCATGGCTAAAATGTATAGACTGAATCAAATCTTGGAGTATCCTGATCCTGTTGGCCATGTATTCTCTGAAGCATTTTGGAAAGCGGGTGTCTTCCCTAACCATCCACGGATATGCACTTTGCTCTCTAAAAAGTTCCCTGAGCATTTCAGCAAATTGCAACTTGAACGTATTGATAAGTTTTCACTGGATAGCTTGCATGATGGTGCTGAACTTCACTTACAGAGCTTAGAGCCATGGATACAGCTGCTGCTCGACTTGATGGCATTTCGAGAACAGGCACTGCGGCTTATATTGGACCTAAGCAGCACTGTAATTACCTTGCTGCCTCATCAGAACTCACTTATTCTGCATGCATTCATGGATCTCTTTTGTGCATTTGTCCGAGTCAATCTCTTCGCAGAGAAGATTCCTAGGAAAATGTTGTTGCAAGTCTACAACCTTCTGCATGCGCTATCAAGAAATGATCGAGACTGTGACTTTTATCACCGCTTGGTGCAATTCATAGACTCTTATGATCCCCCATTGAAAGGCTTACAAGAAGATTTGAATTTTGTCAGCCCAAGGATTGGAGAGGTCCTGGAGGCTGTAGGACCCAGTATTTTTCTATCTGCAGATACGAGGAAGTTGAGAAATGAGGGATTTTTAAGCCCATATCATCCTAGATTCCCAGATATACTTACAAATTCTGCTCATCCCATGAGAGCGCAAGATCTTGCAAATGTTACTTCGTACCGAGAGTGGGTGCTTCTTGGATATCTCGTTTGCCCCGACGAGCTTCTTCGTGTTACTAGCATTGATATCGCCCTGGTCGTGCTAAAGGAAAACTTAGTCGTGACGTTATTCCGGGATGAGGTGAGTTTATATCAAATGGTCTGCGAAAAAGAGTTTGGTATTGGAATTAGTTTTGCATCAGCTGACTCTATTAATCTAACTATGCAGTACATTCTGTTGCACGAAGACTACCAGTTGTATGTTTTACCCCGAGTGTTAGAATCAAAGAAGATGGCTAAGTCTGGACGGACTAAACAAAAGGAAGCTGATCTAGAATATAGTGTAGCAAAACAAGTTGAGAAAATGATCAGTGAGGTGCATGAGCAAGCATTGCAATTATGTGATACCATACACCGAGAAAGGAGAATATTGCTGAAGCAGGAAATAGGGCGGATGGTTTTATTTTTCACTGATCAACCAAGTTTGCTGGCTCCAAACATTCAGATGGTATTCTCTGCGTTGGCTTTGGCCCAATCTGAGGTTCTCTGGTATTTTCAACATGCTGGTATTGCATCCTCAAGATCCAAAGCTGCTCGAGTGATACCAGTAGACATAGATCCAAATGATCCTACCATTGGCTTCCTGCTCGATGGAATGGACCGTCTCTGCTGTTTAGTGCGCAAGTACATCTCAGCTGCTCGAGGTTATGCACTATCATACCTTTCTTCATCTGCTGGAAGGATTCGTTACTTGATGGGTACTCCTGGAATTGTGGCTCTGGACCTTGATCCTACCTTGAAAGGCCTTTTTCAGCGTATTGTGCAACATCTCGAAAGTATACCCAAGGCGCAGGGAGAAAATGTTTCTGCAATCACATGTGACCTATCTGATTTCCGGAAAGATTGGTTGTCCATTTTGATGATTGTCACTTCTTCTCGATCATCGATAAACATCAGGCATTTGGAAAAAGCCACTGTCTCTACTGGGAAGGAGGGCTTGCTATCGGAAGGAAATGCTGCATATAACTGGTCCAGATGTGTTGATGAGTTAGAGTCTCAATTGTCAAAGCATGGAAGTCTGAAGAAATTATATTTCTACCATCAACACCTCACAACTGTTTTCAGAAATACAATGTTTGGACCAGAAGGACGTCCTCAGCATTGTTGTGCATGGCTCAGTGTGGCTAGTAGTTTCCCGGAATGTGCTTCACTAATAATTCCTGAGGAGGTTACCAAATTTGGGCGAGATGCTGTGCTTTACGTCGAGTCTCTTATTGAATCAATAATGGGGGGACTGGAGGGCCTAATTAATATTCTTGATTCAGAAGGCGGGTTTGGCGCTCTAGAATCTCAGCTTCTTCCAGAACAAGCTGCAGCGTATCTGAATAATGCATCAAGAATTTCTGCTCCTTCAGTGAAATCCCCCAGGGTTGTAGGCGGGTTTACTTTGCCTGGCCATGAGAGCTATCCTGAGAATAATAAGTCTATAAAGATGTTGGAAGCTGCAATTCAAAGGTTGACTAATTTGTGTTCAATTCTGAACGACATGGAGCCTATTTGTGTTATAAACCACGTATTTGTTCTAAGAGAGTACATGAGAGAATGCATCCTTGGGAACTTCAAGAGAAGATTTCTTACTGCTCTACAAACCGATAACGATCTCCAGCGGCCTTCTGTTTTGGAATCTCTTATCAGGCGGCATATGGGCATAGTTCACTTGGCAGAGCAGCACGTTAGCATGGACCTAACCCAAGGCATCAGAGAAATTTTACTCACAGAAGCCTTTTCAGGGCCTGTGTCATCTTTGCATACATTCGAAAAACCTGCTGAACAACAGCAAACCACTGGATCTGCAGTTGAAGTTGTATGCAACTGGTACATGGACAATATCATCAAGGATGTGTCTGGCGCAGGAATCCTCTTCGCTCCGAGGCACAAATACTTCAAAAGCACTAGACCAGTTGGAGGGTATTTTGCAGAGTCAGTCACCGACCTCAAGGAATTACAGGCGTTTGTTCGTATCTTCGGCGGCTATGGAGTTGACCGGCTGGATAGGATGATGAAAGTACATACAGCTGCCCTGGTAAACTGCATAGAAACTTCTCTGAGATCAAACCGGGAATTGATTGAAGCAGCTGCTGCAAGTATGCATTCTGGTGACAGAGTGGAGAGAGACGCATCTGTTAGGCAGATAGTTGATTTGGATACCGTAATAGGATTTTGTATTGAAGCTGGTCAAGCGTTGGCTTTTGATGACCTCCTCGCTGAAGCCTCTGGTGCTGTTCTTGAAGACAATGCATCCTTGATACACTCGATGATCTCTGGCATCGTTGAGCACATACCTGAAGAAATACCTGAAAAGAAAGAAATCAGAAGGATCAAAGGCGTGGCAAATGGTGTTGGCGTAGCAGGTGATCACGACTCTGAATGGGTTAGATTAATCCTAGAAGAGGTAGGAGGTGCAAATGACAACTCATGGAGTTTGTTACCTTACTTTTTTGCCTCTTTCATGACCTCAAACGCCTGGAACACTACTGGCTTCAACATCGAGACTGGCGGATTCAGCAACAATATTCATTGCTTGGCTCGGTGTATTAGTGCTGTCATCGCTGGAAGTGAGTACGTAAGGTTACAACGTGAATACCAGCAGCAGCATCAGTCCCTCTCGAATGGTCATCACTCTAGTGAAAATCTCGACTCAGAGTTTCCACCCCGTGTAACTGCTGAAGCAAGCATTAAATCCTCAATGTTACTCTTTGTAAAATTCGCTGCATCGATCGTGCTAGATTCATGGAGCGAAGCCAACAGATCGCATCTCGTGGCTAAGCTTATCTTCCTGGACCAACTCTGTGAGATCTCACCATACCTCCCTAGAAGCTCCCTCGAATCACATGTCCCGTACACCATCCTCAGGTCAATCTACACCCAATACTACTCCAACACACCTTCCACACCGCTCTCAACAGCATCCCCGTACCACTCCCCATCCGTATCGCTCATCCACGCTTCTCCCTCCATGAAAAACTCCACCACTCCCCAGCGTGGTAGTGGTAGCGGCAGTAGCTCTACAGCTGCCCCTGATTCAGGCTACTTCAAAGGCTCATCATCTTCACTATACGGTCAGGAACACTACACCGAATCCGAAACTGGAAACTCAAGAAACAATGAGAACAACAACAACAACAAACAAAGGGGAAGTTCTCGTCGTTCCGGACCATTGGATTACAGCTCGAGCCATAAAGGAGGGTCAGGATCAAACAGCACAGGTCCTAGTCCTCTTCCTAGGTTTGCTGTCTCAAGATCTGGTCCCATCTCATATAAACAGCATAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006355 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008150 GO:0009653 GO:0009825 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010090 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010638 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0030029 GO:0030036 GO:0030154 GO:0030832 GO:0030833 GO:0030838 GO:0031209 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031984 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0032991 GO:0033043 GO:0040007 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045010 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051493 GO:0051495 GO:0060255 GO:0065007 GO:0065008 GO:0071840 GO:0080090 GO:0090066 GO:0090558 GO:0090626 GO:0097435 GO:0098827 GO:0110053 GO:1902680 GO:1902903 GO:1902905 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04810 map04810

Pfam Domains

Protein Families

Nckap1

Protein Analysis

1425

Amino Acids

158.91

Weight (kDa)

6.1

Isoelectric Point (pI)

48.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nckap1	PF09735	73 - 1265	0.0	Nck-associated protein 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008534)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35110 AT2G35110
fragaria_vesca	FvH4_3g12410 FvH4_3g12410
malus_domestica	MD05G1250500.v1.1 MD10G1231300.v1.1
prunus_persica	Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1
pyrus_communis	pycom05g22840 pycom10g19450
rosa_chinensis	RchiOBHm_Chr5g0020301
rosa_laevigata	RLG00000032516
rosa_multiflora	Rmu_sc0002087.1_g000004 Rmu_sc0007157.1_g000007
rosa_roxburghii	Rroxscaffold_1G00057910
rosa_rugosa	Rorug05G0058400
rosa_samantha	Rh5AG148800 Rh5BG148400 Rh5CG159900 Rh5DG147200
rosa_wichuraiana	Rw5G013180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2510
AarI	CACCTGC	1 cut(s)	3344
AatII	GACGTC	1 cut(s)	2098
Acc16I	TGCGCA	1 cut(s)	1625
Acc36I	ACCTGC	2 cut(s)	2776, 3344
AccB7I	CCANNNNNTGG	1 cut(s)	800
AccBSI	CCGCTC	1 cut(s)	3901
AccI	GTMKAC	3 cut(s)	666, 1554, 1783
AccIII	TCCGGA	2 cut(s)	1839, 4154
AcoI	YGGCCR	2 cut(s)	286, 2398
AcsI	RAATTY	7 cut(s)	7, 781, 916, 2180, 2343, 2709, 3707
AcuI	CTGAAG	7 cut(s)	321, 1400, 2045, 2340, 3231, 3309, 3690
AcyI	GRCGYC	1 cut(s)	2095
AdeI	CACNNNGTG	1 cut(s)	3659
AflIII	ACRYGT	3 cut(s)	163, 1820, 3835
AjnI	CCWGG	8 cut(s)	810, 1038, 1708, 2369, 2395, 3024, 3467, 3780
AleI	CACNNNNGTG	1 cut(s)	3627
Alw21I	GWGCWC	2 cut(s)	146, 3282
Alw26I	GTCTC	9 cut(s)	236, 696, 1613, 1927, 2004, 2217, 3111, 3488, 4243
AlwNI	CAGNNNCTG	4 cut(s)	481, 514, 2738, 3083
Ama87I	CYCGRG	4 cut(s)	1215, 1539, 1644, 4174
Aor13HI	TCCGGA	2 cut(s)	1839, 4154
AoxI	GGCC	7 cut(s)	286, 1473, 1747, 2245, 2398, 2612, 2735
ApoI	RAATTY	7 cut(s)	7, 781, 916, 2180, 2343, 2709, 3707
ArsI	GACNNNNNNTTYG	2 cut(s)	3822, 3854
AseI	ATTAAT	3 cut(s)	1158, 2253, 3383
AspA2I	CCTAGG	2 cut(s)	644, 4226
AspLEI	GCGC	6 cut(s)	685, 940, 1626, 1795, 2285, 2855
AsuC2I	CCSGG	3 cut(s)	1079, 2141, 3062
AsuHPI	GGTGA	6 cut(s)	710, 1099, 2923, 3113, 3368, 3795
AsuII	TTCGAA	1 cut(s)	2760
AvaI	CYCGRG	4 cut(s)	1215, 1539, 1644, 4174
AvrII	CCTAGG	2 cut(s)	644, 4226
AxyI	CCTNAGG	2 cut(s)	2166, 3854
BalI	TGGCCA	2 cut(s)	288, 2400
BanII	GRGCYC	1 cut(s)	146
BarI	GAAGNNNNNNTAC	2 cut(s)	2563, 2595
BauI	CACGAG	1 cut(s)	3761
BbsI	GAAGAC	4 cut(s)	123, 316, 1197, 3240
Bbv12I	GWGCWC	2 cut(s)	146, 3282
BbvCI	CCTCAGC	1 cut(s)	2100
BcgI	CGANNNNNNTGC	4 cut(s)	1015, 1049, 2750, 2784
BciT130I	CCWGG	8 cut(s)	812, 1040, 1710, 2371, 2397, 3026, 3469, 3782
BciVI	GTATCC	3 cut(s)	282, 465, 3142
BclI	TGATCA	3 cut(s)	1311, 1417, 3358
BcnI	CCSGG	3 cut(s)	1079, 2141, 3062
BcoDI	GTCTC	9 cut(s)	236, 696, 1613, 1927, 2004, 2217, 3111, 3488, 4243
BfmI	CTRYAG	5 cut(s)	819, 843, 2316, 2796, 4011
BfoI	RGCGCY	1 cut(s)	2286
BfuAI	ACCTGC	2 cut(s)	2776, 3344
BfuI	GTATCC	3 cut(s)	282, 465, 3142
BglII	AGATCT	3 cut(s)	943, 3798, 4244
BlnI	CCTAGG	2 cut(s)	644, 4226
BlpI	GCTNAGC	1 cut(s)	3768
BmcAI	AGTACT	1 cut(s)	101
BmeT110I	CYCGRG	4 cut(s)	1215, 1539, 1644, 4174
BmiI	GGNNCC	6 cut(s)	108, 175, 826, 1437, 2496, 4252
BmrI	ACTGGG	2 cut(s)	822, 1938
BmuI	ACTGGG	2 cut(s)	822, 1938
BpiI	GAAGAC	4 cut(s)	123, 316, 1197, 3240
BplI	GAGNNNNNCTC	2 cut(s)	609, 641
BpmI	CTGGAG	3 cut(s)	833, 2261, 2591
Bpu10I	CCTNAGC	3 cut(s)	372, 533, 2100
Bpu1102I	GCTNAGC	1 cut(s)	3768
Bpu14I	TTCGAA	1 cut(s)	2760
BpuEI	CTTGAG	5 cut(s)	15, 1507, 2924, 4084, 4225
BpuMI	CCSGG	3 cut(s)	1079, 2141, 3062
Bsa29I	ATCGAT	2 cut(s)	1889, 3719
BsaAI	YACGTR	2 cut(s)	2518, 3568
BsaBI	GATNNNNATC	2 cut(s)	1895, 2601
BsaHI	GRCGYC	1 cut(s)	2095
BsaI	GGTCTC	1 cut(s)	236
BsaWI	WCCGGW	2 cut(s)	1839, 4154
BsaXI	ACNNNNNCTCC	4 cut(s)	1791, 1821, 3795, 3825
Bse118I	RCCGGY	1 cut(s)	2988
Bse21I	CCTNAGG	2 cut(s)	2166, 3854
Bse3DI	GCAATG	2 cut(s)	1334, 3520
Bse8I	GATNNNNATC	2 cut(s)	1895, 2601
BseAI	TCCGGA	2 cut(s)	1839, 4154
BseBI	CCWGG	8 cut(s)	812, 1040, 1710, 2371, 2397, 3026, 3469, 3782
BseCI	ATCGAT	2 cut(s)	1889, 3719
BseJI	GATNNNNATC	2 cut(s)	1895, 2601
BseMI	GCAATG	2 cut(s)	1334, 3520
BseRI	GAGGAG	2 cut(s)	2183, 3194
BseYI	CCCAGC	1 cut(s)	3981
Bsh1285I	CGRYCG	1 cut(s)	3723
BshFI	GGCC	7 cut(s)	288, 1475, 1749, 2247, 2400, 2614, 2737
BshVI	ATCGAT	2 cut(s)	1889, 3719
BsiEI	CGRYCG	1 cut(s)	3723
BsiHKAI	GWGCWC	2 cut(s)	146, 3282
BsiHKCI	CYCGRG	4 cut(s)	1215, 1539, 1644, 4174
BsiSI	CCGG	6 cut(s)	1078, 1840, 2141, 2989, 3061, 4155
BslFI	GGGAC	6 cut(s)	31, 120, 2250, 3589, 3824, 4236
BsmAI	GTCTC	9 cut(s)	236, 696, 1613, 1927, 2004, 2217, 3111, 3488, 4243
BsmBI	CGTCTC	2 cut(s)	1613, 3111
BsmFI	GGGAC	6 cut(s)	31, 120, 2250, 3589, 3824, 4236
BsmI	GAATGC	3 cut(s)	587, 2552, 3094
BsnI	GGCC	7 cut(s)	288, 1475, 1749, 2247, 2400, 2614, 2737
Bso31I	GGTCTC	1 cut(s)	236
BsoBI	CYCGRG	4 cut(s)	1215, 1539, 1644, 4174
Bsp119I	TTCGAA	1 cut(s)	2760
Bsp1286I	GDGCHC	2 cut(s)	146, 3282
Bsp13I	TCCGGA	2 cut(s)	1839, 4154
Bsp1720I	GCTNAGC	1 cut(s)	3768
Bsp19I	CCATGG	1 cut(s)	467
BspANI	GGCC	7 cut(s)	288, 1475, 1749, 2247, 2400, 2614, 2737
BspDI	ATCGAT	2 cut(s)	1889, 3719
BspEI	TCCGGA	2 cut(s)	1839, 4154
BspHI	TCATGA	1 cut(s)	3453
BspLI	GGNNCC	6 cut(s)	108, 175, 826, 1437, 2496, 4252
BspMAI	CTGCAG	3 cut(s)	847, 2320, 2800
BspMI	ACCTGC	2 cut(s)	2776, 3344
BspT104I	TTCGAA	1 cut(s)	2760
BspTNI	GGTCTC	1 cut(s)	236
BsrBI	CCGCTC	1 cut(s)	3901
BsrDI	GCAATG	2 cut(s)	1334, 3520
BsrFI	RCCGGY	1 cut(s)	2988
BssAI	RCCGGY	1 cut(s)	2988
BssNAI	GTATAC	1 cut(s)	1784
BssNI	GRCGYC	1 cut(s)	2095
BssSI	CACGAG	1 cut(s)	3761
BssT1I	CCWWGG	7 cut(s)	467, 644, 794, 1788, 2554, 2695, 4226
Bst1107I	GTATAC	1 cut(s)	1784
Bst2BI	CACGAG	1 cut(s)	3761
Bst2UI	CCWGG	8 cut(s)	812, 1040, 1710, 2371, 2397, 3026, 3469, 3782
Bst4CI	ACNGT	8 cut(s)	230, 544, 707, 1607, 1921, 2062, 3154, 4064
Bst6I	CTCTTC	4 cut(s)	632, 2870, 3387, 4227
BstACI	GRCGYC	1 cut(s)	2095
BstAPI	GCANNNNNTGC	2 cut(s)	1336, 3083
BstBAI	YACGTR	2 cut(s)	2518, 3568
BstBI	TTCGAA	1 cut(s)	2760
BstC8I	GCNNGC	7 cut(s)	428, 585, 681, 1332, 1434, 1943, 3676
BstDSI	CCRYGG	2 cut(s)	338, 467
BstEII	GGTNACC	1 cut(s)	2173
BstENI	CCTNNNNNAGG	2 cut(s)	3392, 4026
BstH2I	RGCGCY	1 cut(s)	2286
BstHHI	GCGC	6 cut(s)	685, 940, 1626, 1795, 2285, 2855
BstMAI	GTCTC	9 cut(s)	236, 696, 1613, 1927, 2004, 2217, 3111, 3488, 4243
BstMCI	CGRYCG	1 cut(s)	3723
BstNI	CCWGG	8 cut(s)	812, 1040, 1710, 2371, 2397, 3026, 3469, 3782
BstNSI	RCATGY	6 cut(s)	167, 587, 683, 1508, 1824, 3839
BstPI	GGTNACC	1 cut(s)	2173
BstSFI	CTRYAG	5 cut(s)	819, 843, 2316, 2796, 4011
BstSNI	TACGTA	1 cut(s)	3568
BstV2I	GAAGAC	4 cut(s)	123, 316, 1197, 3240
BstX2I	RGATCY	7 cut(s)	595, 943, 1526, 1561, 2792, 3798, 4244
BstYI	RGATCY	7 cut(s)	595, 943, 1526, 1561, 2792, 3798, 4244
BstZ17I	GTATAC	1 cut(s)	1784
Bsu15I	ATCGAT	2 cut(s)	1889, 3719
Bsu36I	CCTNAGG	2 cut(s)	2166, 3854
BsuI	GTATCC	3 cut(s)	282, 465, 3142
BsuRI	GGCC	7 cut(s)	288, 1475, 1749, 2247, 2400, 2614, 2737
BsuTUI	ATCGAT	2 cut(s)	1889, 3719
BtgI	CCRYGG	2 cut(s)	338, 467
BtgZI	GCGATG	1 cut(s)	3535
BtsI	GCAGTG	1 cut(s)	512
BveI	ACCTGC	2 cut(s)	2776, 3344
Cac8I	GCNNGC	7 cut(s)	428, 585, 681, 1332, 1434, 1943, 3676
CaiI	CAGNNNCTG	4 cut(s)	481, 514, 2738, 3083
CciI	TCATGA	1 cut(s)	3453
CfoI	GCGC	6 cut(s)	685, 940, 1626, 1795, 2285, 2855
Cfr10I	RCCGGY	1 cut(s)	2988
ClaI	ATCGAT	2 cut(s)	1889, 3719
CseI	GACGC	1 cut(s)	3128
DraIII	CACNNNGTG	1 cut(s)	3659
EaeI	YGGCCR	2 cut(s)	286, 2398
Eam1104I	CTCTTC	4 cut(s)	632, 2870, 3387, 4227
EarI	CTCTTC	4 cut(s)	632, 2870, 3387, 4227
EciI	GGCGGA	2 cut(s)	1411, 3516
Ecl136II	GAGCTC	1 cut(s)	144
Eco105I	TACGTA	1 cut(s)	3568
Eco130I	CCWWGG	7 cut(s)	467, 644, 794, 1788, 2554, 2695, 4226
Eco147I	AGGCCT	1 cut(s)	1749
Eco24I	GRGCYC	1 cut(s)	146
Eco31I	GGTCTC	1 cut(s)	236
Eco32I	GATATC	2 cut(s)	989, 1033
Eco53kI	GAGCTC	1 cut(s)	144
Eco57I	CTGAAG	7 cut(s)	321, 1400, 2045, 2340, 3231, 3309, 3690
Eco81I	CCTNAGG	2 cut(s)	2166, 3854
Eco88I	CYCGRG	4 cut(s)	1215, 1539, 1644, 4174
Eco91I	GGTNACC	1 cut(s)	2173
EcoICRI	GAGCTC	1 cut(s)	144
EcoNI	CCTNNNNNAGG	2 cut(s)	3392, 4026
EcoO109I	RGGNCCY	7 cut(s)	80, 107, 808, 824, 2245, 2735, 4211
EcoO65I	GGTNACC	1 cut(s)	2173
EcoRI	GAATTC	1 cut(s)	7
EcoRII	CCWGG	8 cut(s)	810, 1038, 1708, 2369, 2395, 3024, 3467, 3780
EcoRV	GATATC	2 cut(s)	989, 1033
EcoT14I	CCWWGG	7 cut(s)	467, 644, 794, 1788, 2554, 2695, 4226
EcoT22I	ATGCAT	5 cut(s)	589, 2338, 2552, 3096, 3244
EcoT38I	GRGCYC	1 cut(s)	146
ErhI	CCWWGG	7 cut(s)	467, 644, 794, 1788, 2554, 2695, 4226
Esp3I	CGTCTC	2 cut(s)	1613, 3111
FalI	AAGNNNNNCTT	6 cut(s)	1043, 1075, 1925, 1957, 3761, 3793
FaqI	GGGAC	6 cut(s)	31, 120, 2250, 3589, 3824, 4236
FauI	CCCGC	3 cut(s)	310, 2267, 2375
FauNDI	CATATG	1 cut(s)	2646
FbaI	TGATCA	3 cut(s)	1311, 1417, 3358
FblI	GTMKAC	3 cut(s)	666, 1554, 1783
FriOI	GRGCYC	1 cut(s)	146
FspI	TGCGCA	1 cut(s)	1625
GlaI	GCGC	6 cut(s)	684, 939, 1625, 1794, 2284, 2854
GsaI	CCCAGC	1 cut(s)	3985
GsuI	CTGGAG	3 cut(s)	833, 2261, 2591
HaeII	RGCGCY	1 cut(s)	2286
HaeIII	GGCC	7 cut(s)	288, 1475, 1749, 2247, 2400, 2614, 2737
HapII	CCGG	6 cut(s)	1078, 1840, 2141, 2989, 3061, 4155
HgaI	GACGC	1 cut(s)	3128
HhaI	GCGC	6 cut(s)	685, 940, 1626, 1795, 2285, 2855
Hin1I	GRCGYC	1 cut(s)	2095
Hin6I	GCGC	6 cut(s)	683, 938, 1624, 1793, 2283, 2853
HinP1I	GCGC	6 cut(s)	683, 938, 1624, 1793, 2283, 2853
HincII	GTYRAC	2 cut(s)	2463, 2986
HindII	GTYRAC	2 cut(s)	2463, 2986
HindIII	AAGCTT	1 cut(s)	3770
HpaII	CCGG	6 cut(s)	1078, 1840, 2141, 2989, 3061, 4155
HphI	GGTGA	6 cut(s)	710, 1099, 2923, 3113, 3368, 3795
Hpy166II	GTNNAC	9 cut(s)	667, 1555, 1784, 2388, 2463, 2659, 2986, 3031, 3846
Hpy8I	GTNNAC	9 cut(s)	667, 1555, 1784, 2388, 2463, 2659, 2986, 3031, 3846
Hpy99I	CGWCG	3 cut(s)	1007, 2210, 4152
HpyCH4III	ACNGT	8 cut(s)	230, 544, 707, 1607, 1921, 2062, 3154, 4064
HpyCH4IV	ACGT	8 cut(s)	400, 1070, 2095, 2205, 2517, 2676, 3567, 3580
HpySE526I	ACGT	8 cut(s)	400, 1070, 2095, 2205, 2517, 2676, 3567, 3580
Hsp92I	GRCGYC	1 cut(s)	2095
HspAI	GCGC	6 cut(s)	683, 938, 1624, 1793, 2283, 2853
Kpn2I	TCCGGA	2 cut(s)	1839, 4154
Ksp22I	TGATCA	3 cut(s)	1311, 1417, 3358
LmnI	GCTCC	7 cut(s)	141, 1441, 2356, 2494, 2875, 3738, 3827
MaeII	ACGT	8 cut(s)	400, 1070, 2095, 2205, 2517, 2676, 3567, 3580
MbiI	CCGCTC	1 cut(s)	3901
MfeI	CAATTG	1 cut(s)	2003
MflI	RGATCY	7 cut(s)	595, 943, 1526, 1561, 2792, 3798, 4244
MhlI	GDGCHC	2 cut(s)	146, 3282
MlsI	TGGCCA	2 cut(s)	288, 2400
MluNI	TGGCCA	2 cut(s)	288, 2400
MlyI	GAGTC	8 cut(s)	627, 734, 1145, 2006, 2219, 2933, 3359, 3635
MmeI	TCCRAC	3 cut(s)	2421, 2887, 3906
Mox20I	TGGCCA	2 cut(s)	288, 2400
Mph1103I	ATGCAT	5 cut(s)	589, 2338, 2552, 3096, 3244
MroI	TCCGGA	2 cut(s)	1839, 4154
MscI	TGGCCA	2 cut(s)	288, 2400
MseI	TTAA	5 cut(s)	878, 1158, 2253, 3383, 3681
MslI	CAYNNNNRTG	8 cut(s)	168, 343, 435, 1448, 1862, 2109, 3099, 3627
Msp20I	TGGCCA	2 cut(s)	288, 2400
MspA1I	CMGCKG	7 cut(s)	478, 1148, 1640, 2611, 3020, 3080, 4016
MspI	CCGG	6 cut(s)	1078, 1840, 2141, 2989, 3061, 4155
MunI	CAATTG	1 cut(s)	2003
Mva1269I	GAATGC	3 cut(s)	587, 2552, 3094
MvaI	CCWGG	8 cut(s)	812, 1040, 1710, 2371, 2397, 3026, 3469, 3782
NciI	CCSGG	3 cut(s)	1079, 2141, 3062
NcoI	CCATGG	1 cut(s)	467
NdeI	CATATG	1 cut(s)	2646
NlaIV	GGNNCC	6 cut(s)	108, 175, 826, 1437, 2496, 4252
NmuCI	GTSAC	6 cut(s)	707, 1066, 1823, 1870, 2929, 3101
NsbI	TGCGCA	1 cut(s)	1625
NsiI	ATGCAT	5 cut(s)	589, 2338, 2552, 3096, 3244
NspI	RCATGY	6 cut(s)	167, 587, 683, 1508, 1824, 3839
NspV	TTCGAA	1 cut(s)	2760
OliI	CACNNNNGTG	1 cut(s)	3627
PaeI	GCATGC	2 cut(s)	587, 683
PaeR7I	CTCGAG	3 cut(s)	1539, 1644, 4174
PagI	TCATGA	1 cut(s)	3453
PaqCI	CACCTGC	1 cut(s)	3344
PasI	CCCWGGG	1 cut(s)	2370
PceI	AGGCCT	1 cut(s)	1749
PciI	ACATGT	3 cut(s)	163, 1820, 3835
PcsI	WCGNNNNNNNCGW	2 cut(s)	999, 1041
PctI	GAATGC	3 cut(s)	587, 2552, 3094
PflMI	CCANNNNNTGG	1 cut(s)	800
PfoI	TCCNGGA	5 cut(s)	810, 1077, 1708, 2139, 3780
Ple19I	CGATCG	1 cut(s)	3723
PleI	GAGTC	8 cut(s)	626, 734, 1145, 2005, 2218, 2932, 3359, 3635
PpsI	GAGTC	8 cut(s)	626, 734, 1145, 2005, 2218, 2932, 3359, 3635
Ppu21I	YACGTR	2 cut(s)	2518, 3568
PpuMI	RGGWCCY	5 cut(s)	80, 107, 808, 824, 4211
PscI	ACATGT	3 cut(s)	163, 1820, 3835
PshBI	ATTAAT	3 cut(s)	1158, 2253, 3383
PsiI	TTATAA	1 cut(s)	2510
Psp124BI	GAGCTC	1 cut(s)	146
Psp5II	RGGWCCY	5 cut(s)	80, 107, 808, 824, 4211
Psp6I	CCWGG	8 cut(s)	810, 1038, 1708, 2369, 2395, 3024, 3467, 3780
PspEI	GGTNACC	1 cut(s)	2173
PspFI	CCCAGC	1 cut(s)	3981
PspGI	CCWGG	8 cut(s)	810, 1038, 1708, 2369, 2395, 3024, 3467, 3780
PspN4I	GGNNCC	6 cut(s)	108, 175, 826, 1437, 2496, 4252
PspPPI	RGGWCCY	5 cut(s)	80, 107, 808, 824, 4211
PspXI	VCTCGAGB	3 cut(s)	1539, 1644, 4174
PsrI	GAACNNNNNNTAC	2 cut(s)	435, 467
PstI	CTGCAG	3 cut(s)	847, 2320, 2800
PstNI	CAGNNNCTG	4 cut(s)	481, 514, 2738, 3083
PsuI	RGATCY	7 cut(s)	595, 943, 1526, 1561, 2792, 3798, 4244
PvuI	CGATCG	1 cut(s)	3723
PvuII	CAGCTG	6 cut(s)	478, 1148, 1640, 3020, 3080, 4016
RseI	CAYNNNNRTG	8 cut(s)	168, 343, 435, 1448, 1862, 2109, 3099, 3627
SacI	GAGCTC	1 cut(s)	146
SaqAI	TTAA	5 cut(s)	878, 1158, 2253, 3383, 3681
ScaI	AGTACT	1 cut(s)	101
SchI	GAGTC	8 cut(s)	627, 734, 1145, 2006, 2219, 2933, 3359, 3635
SduI	GDGCHC	2 cut(s)	146, 3282
SfcI	CTRYAG	5 cut(s)	819, 843, 2316, 2796, 4011
Sfr274I	CTCGAG	3 cut(s)	1539, 1644, 4174
SfuI	TTCGAA	1 cut(s)	2760
SlaI	CTCGAG	3 cut(s)	1539, 1644, 4174
SmiMI	CAYNNNNRTG	8 cut(s)	168, 343, 435, 1448, 1862, 2109, 3099, 3627
SmlI	CTYRAG	8 cut(s)	30, 1522, 1539, 1644, 2939, 4099, 4174, 4240
SmoI	CTYRAG	8 cut(s)	30, 1522, 1539, 1644, 2939, 4099, 4174, 4240
SnaBI	TACGTA	1 cut(s)	3568
SphI	GCATGC	2 cut(s)	587, 683
SseBI	AGGCCT	1 cut(s)	1749
SspI	AATATT	4 cut(s)	20, 1374, 2257, 3517
SstI	GAGCTC	1 cut(s)	146
StuI	AGGCCT	1 cut(s)	1749
StyI	CCWWGG	7 cut(s)	467, 644, 794, 1788, 2554, 2695, 4226
TaaI	ACNGT	8 cut(s)	230, 544, 707, 1607, 1921, 2062, 3154, 4064
TaiI	ACGT	8 cut(s)	403, 1073, 2098, 2208, 2520, 2679, 3570, 3583
TatI	WGTACW	5 cut(s)	99, 1173, 1629, 2535, 3010
TauI	GCSGC	5 cut(s)	520, 2614, 2644, 2976, 4002
Tru1I	TTAA	5 cut(s)	878, 1158, 2253, 3383, 3681
Tru9I	TTAA	5 cut(s)	878, 1158, 2253, 3383, 3681
TseFI	GTSAC	6 cut(s)	707, 1066, 1823, 1870, 2929, 3101
Tsp45I	GTSAC	6 cut(s)	707, 1066, 1823, 1870, 2929, 3101
TspDTI	ATGAA	9 cut(s)	51, 580, 724, 1662, 3020, 3442, 3509, 3723, 3977
TspGWI	ACGGA	3 cut(s)	355, 1267, 3922
Van91I	CCANNNNNTGG	1 cut(s)	800
VspI	ATTAAT	3 cut(s)	1158, 2253, 3383
XagI	CCTNNNNNAGG	2 cut(s)	3392, 4026
XapI	RAATTY	7 cut(s)	7, 781, 916, 2180, 2343, 2709, 3707
XbaI	TCTAGA	2 cut(s)	1276, 2287
XceI	RCATGY	6 cut(s)	167, 587, 683, 1508, 1824, 3839
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3989
XhoI	CTCGAG	3 cut(s)	1539, 1644, 4174
XmaJI	CCTAGG	2 cut(s)	644, 4226
XmiI	GTMKAC	3 cut(s)	666, 1554, 1783
ZraI	GACGTC	1 cut(s)	2096
ZrmI	AGTACT	1 cut(s)	101
Zsp2I	ATGCAT	5 cut(s)	589, 2338, 2552, 3096, 3244

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.