Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0008534

Physical Location & Seq

Genomic Coordinates Forward (+)

15506387 .. 15519634

Gene Structure 13248 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G013180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4179 bp

ATGCTCAACAAGTTTGTGTTGATTAACTCAGCTATGGCGAAATCGAGACAACATTTCTCTAGCCAAGATTCATCGTTGTCACCCACTTCTGTGAGATCAAGAGAGTGGGAGGGTCCATCGAGATGGGCTGAGTACTTGGGTCCTGAACCAACTTCGCCCATGACATCTAGGAGCTCCAGGAATGCAGGTCCCGATGGACAGATCCAAAGTTCAGGCGGGTCCCACAAAGGTTTAAACATGCAGTGGGTAGTTCAACTAACTGAAGTAGCAGAAGGTCTTATGGCTAAAATGTATAGGTTAAACCAAACTTTGGATTACCCGGATCCAGCTGGCCATACATTTTCTGAAGCATTTTGGAAAGCCGGTGTGTTCCCCAACCATCCAAGACTTTGCTTGCTACTTTCAAAGAAGTTCCCGGAGCATTATAGCAAGTTACAGCTTGAACGTGTTGATAAGCTTGCCTTGGACGCCTTGCATGGTAATGCAGAGCTTCATTTACAAAGCCTGGAACCATGGGTTCAGCTGCTTCTTGACTTGATGGCATTTCGAGAACAAGCTCTACGGCTTATATTGGATCTAAGCAGTACTGTTATTACCTTGTTGCCTCACCAGAACTCGCTTATACTACATGCATTTATGGATCTCTTCTGTTCATTTGTCCGTGTTAACCTATTTTCCGAGAAGATACCGAGGAAAATGATGCTACAAATGTATAACCTTTTGCATGCGATATCAAGAAATGACCGAGATTGTGATTTCTATCATCGGTTGGTTCAATTTATAGACAGTTATGATCCACCCTTGAAAGGATTGAAAGAAGACCTGAATTTTGTGAGCCCCCGAATTGGAGAGGTGCTAGAGGCTGTGGGTCCTATTATTTTTCTATCAACAGATACAAGAAAGCTCCGAAATGAGGGATTCTTAAGTCCATATCATCCTCGATATCCAGACATACTTACAAATTCTGCACACCCAATGAGAGCTCAGGATTTAGCAAATGTCACCTCATATCGGGAATGGGTATTATTTGGGTACCTTGTTTGTCCAGATGAGCTTCTTCGCGTCACTAGCATTGATATTGCTCTGGTCGTCTTGAAAGAAAATTTAGTTCTTACATTGTTCAGGGATGAGTACATACTTTTGCATGAAGATTATCAGCTATTTGTCCTGCCTCGAATTTTAGAGTCAAAGAAGATGGCAAAATCTGGACGTACAAAGCAAAAAGAAGCAGATTTGGAGTATAGTGTCGCAAAGCAAGTTGAGAAAATGATAAGTGAAGTGCATGAGCAAGCATTACTATCATGTGATGCCATCCACCGTGAGCGGAGGATATTACTGAAGCAAGAGATTGGAAGAATGGTACTTTTTTTCACTGATCAGCCTAGTTTGTTGGCTCCAAACATACAGATGGTATTCTCTGCCCTGGCTTTTGCTCAATGTGAAGTCCTCTGGTATTTCCAACATGTTGGAATTGCATCATCAAAATCTAAAGCTGTTCGAATGGTGTCAGTTGAAATAGATCCAAGTGATCCAACTATTGGGTTTTTGTTAGATGGAATGGACCATCTTTGCTGTTTGGTACGCAAGTATATTGCAGCAATTCGAGGTTATGCATTATCATACCTTTCTTCTTCTGCTGGTAGAATCCGGTTTTTGTTGAATACTCCTGGGATGGTGGCTCTTGACTTAGATGCAAGCTTGAAAAGTCTTTTTCAGAAGATTGTTCAGCACCTTGAGAACATACCAAAACCACAGGGTGAAAATGTATCTGCCATTACATGTGATCTATCTGAATTTCGAAAGGATTGGTTATCAATATTGATGATAGTCACATCATCCCGGTCATCTATAAACATAAGACATTTGGAGAAAGCTACAGTCTCTACAGGGAAAGAAGGCTTACTATCAGAGGGAAACGCTGCCTACAATTGGTCCAGATGTGTCGATGAACTGGAATCTCAATTATCAAAGCATGCGAGTCTTAAGAAGTTGTATTTCTACCATCAGCATCTTACAGCGGTATTCAGGAACACTATGTTTGGCCCAGAAGGGCGCCCACAACATTGCTGTGCATGGCTTGGTGTTGCAAGTAGTTTTCCAGAGTGTGCTTCGCCAATTGTTCCGGAAGAGGTAACCAAGATAGGCAGAGATGCAGTCCTTTATGTTGAATCTCTCATTGAATCTATAATGGGAGGGCTGGAGGGATTAATTAACATCCTAGATTCTGATGGAGGATTTGGTGCGCTAGAGACCCAGCTTCTTCCAGAGCAGGCAGCTTCTTATATGAACTATGCATCAAGAATGTCAATTACTTCAACTAAATCTCCAAAGGGAGCACCTGGTTTCCCTTTGCCTGGCCATGAGAGTCGTCCTGAAAATAATGCTTCTATCAAGATGTTAGAAGCTGCGGTGCAGAGATTGACCAACTTATGCTCAGTTTTGAATGATATGGAGCCTATATGTGTTCTAAACCACGTTTTTGTCTTAAGGGAGTACATGAGAGAGTGCATTCTTGGGAACCTTAGGAGGAGAGTGCTTGCGGTACTGAAAACTGACAATGACCTCCAACGGCCTACAGTTCTTGAGTCGCTTATTCGCAGACACATCAGCATTATCCATCTGGCAGAGCAGCATATTAGCATGGACTTAACTCAGGGTATTCGAGAAGTTTTGCTCTCAGAGGCCTTCTCAGGACCAGTTTCTTCTTTGCATTTGTTTGAAAAACCAGAAGAGCAACATACAGGATCGGCCACTGAAGCTGTCTGCAACTGGTACATTGAAAATATAATCAAGGATATATCAGGTGCTGGAATCCTTTTTGCTCCAATTCACAATTGCTTCAAGAGCACAAGGCCTGTTGGCGGATATTTTGCTGATTCAGTCACTGATCCAAGAGAATTGAAGGCATTTGCTCGCATCTTTGGTGGCTATGGAGTTGACAGGCTAGACAGAATGCTGAAAGAACACACTGCTGCACTTTTAAATTGTATTGACACATCACTGAGATCAAATCGTGATGTGCTAGAGGCAGTTGCTGGTAGCCTGCATTCTGGAGACCGTATAGAAAGAGAGGCTTTAATAAAGCAGATAGTCGATATAGACACGGTGATTGGGTTTTGTGTTCAAGCTGGCTTGGCCCTGGCTTTTGACAGACTTCTAGCTGAAGCTGCTGGCACTGTACTCTTTGAAGGTGCACCCTTGATACATTCATTATTAGCTGGAATATCTAAGCATATACCTGAAGAAATACCTGAAAAGAAAGAAGTCAGGAGATTGAGAGGGGTGGCAAACAACGTTGGTGTAGTCGATAATCATGATTCCCAGTGGGTGAGATTGATCTTAGAAGAGGTTGGGGGTGCAAATGATGGGTCCTGGAGCCTGTTACCCTATTTCTTTGCCACCTTCATGACATCTAATATATGGACTACCACAGCCTTCAACGTGGACACTGGGGGATTCAATAACAATATCCATTGCTTAGCAAGGTGTGTCAGTGCTGTAATTGCAGGAAGTGAGTTCGTTAGAGTGGAACGTGAATATCAGCAAAGACAATCTCTCTCAAATGGCCATGTTGGTGAATCAGTGGACTCAGAAATACAAAATCGTTTGTCAGCTGAAGCTAGCATAAAATCCACAATGCAGCTTTTCGTCAAATTCTCTGCAAGCATTATACTGGATTCTTGGAGTGAAACTAACAGATCTCATCTTGTAGCACAGCTAATTTTCCTGGACCAACTCTGCGAGATCTCACCTTATCTTCCAAGAAGCTCTCTGGAACCCCATGTTCCATATGCCATTCTTCGTTCAATATACAGCCAGTATTATACAAACTCTCCAAATACACCACTTGCATTACTAAGCATTTCACCCCGCCATTCGCCTGCTGTATCACTCTCACATGCATCTCCTGCGGCACGACAACCCCGTGGTGACTCCACTCCTCAGTATGATTCAGGTTATTTCAAAGGATCATCATCCCATGGCCAAGAGCACATTTATGATGGTGGAAGTTCACGAAGCACTGAAAACAGGCAACAGAACTTTCGCCGTTCTGGGCCTTTGGATTATGGCTCAAGCCGTAAAGTTAAGTTTGTGGAGGGCTCAACTTCAGGGAACAGTGGTCCCAGTCCACTACCAAGATTTGCCGTCTCAAGGTCGGGTCCAATATCGTACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006355 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008150 GO:0009653 GO:0009825 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010090 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010638 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0030029 GO:0030036 GO:0030154 GO:0030832 GO:0030833 GO:0030838 GO:0031209 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031984 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0032991 GO:0033043 GO:0040007 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045010 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051493 GO:0051495 GO:0060255 GO:0065007 GO:0065008 GO:0071840 GO:0080090 GO:0090066 GO:0090558 GO:0090626 GO:0097435 GO:0098827 GO:0110053 GO:1902680 GO:1902903 GO:1902905 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04810 map04810

Pfam Domains

Protein Families

Nckap1

Protein Analysis

1392

Amino Acids

155.58

Weight (kDa)

6.5

Isoelectric Point (pI)

47.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nckap1	PF09735	86 - 1276	0.0	Nck-associated protein 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008534)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35110 AT2G35110
fragaria_vesca	FvH4_3g12410 FvH4_3g12410
malus_domestica	MD05G1250500.v1.1 MD10G1231300.v1.1
prunus_persica	Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1
pyrus_communis	pycom05g22840 pycom10g19450
rosa_chinensis	RchiOBHm_Chr5g0020301
rosa_laevigata	RLG00000032516
rosa_multiflora	Rmu_sc0002087.1_g000004 Rmu_sc0007157.1_g000007
rosa_roxburghii	Rroxscaffold_1G00057910
rosa_rugosa	Rorug05G0058400
rosa_samantha	Rh5AG148800 Rh5BG148400 Rh5CG159900 Rh5DG147200
rosa_wichuraiana	Rw5G013180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	176
Acc65I	GGTACC	1 cut(s)	1032
AccB1I	GGYRCC	2 cut(s)	1032, 2052
AccB7I	CCANNNNNTGG	2 cut(s)	310, 1538
AccBSI	CCGCTC	1 cut(s)	1324
AccII	CGCG	1 cut(s)	1062
AccIII	TCCGGA	1 cut(s)	2122
AciI	CCGC	8 cut(s)	216, 1324, 2018, 2408, 2540, 2862, 3871, 3911
AclI	AACGTT	1 cut(s)	3294
AcoI	YGGCCR	5 cut(s)	331, 2356, 2748, 3565, 3982
AcsI	RAATTY	6 cut(s)	826, 961, 1102, 1176, 1793, 3653
AcuI	CTGAAG	8 cut(s)	282, 366, 1358, 2775, 3183, 3261, 3636, 4092
AcyI	GRCGYC	2 cut(s)	468, 2053
AdeI	CACNNNGTG	1 cut(s)	1757
AflII	CTTAAG	3 cut(s)	922, 1982, 2485
AflIII	ACRYGT	3 cut(s)	445, 1462, 1778
AjnI	CCWGG	9 cut(s)	176, 504, 1422, 1666, 2338, 2353, 3138, 3371, 3726
AleI	CACNNNNGTG	1 cut(s)	89
Alw21I	GWGCWC	6 cut(s)	176, 985, 2338, 2849, 3196, 3993
Alw26I	GTCTC	5 cut(s)	40, 1885, 2243, 3048, 4153
Alw44I	GTGCAC	1 cut(s)	3192
AlwNI	CAGNNNCTG	3 cut(s)	2807, 2885, 3035
Aor13HI	TCCGGA	1 cut(s)	2122
ApaLI	GTGCAC	1 cut(s)	3192
ApeKI	GCWGC	9 cut(s)	523, 1595, 1919, 2273, 2405, 2629, 2972, 3167, 3640
ApoI	RAATTY	6 cut(s)	826, 961, 1102, 1176, 1793, 3653
ArsI	GACNNNNNNTTYG	4 cut(s)	812, 844, 3353, 3385
AseI	ATTAAT	1 cut(s)	2207
Asp718I	GGTACC	1 cut(s)	1032
AspLEI	GCGC	2 cut(s)	2055, 2245
AsuC2I	CCSGG	3 cut(s)	320, 416, 1840
AsuII	TTCGAA	2 cut(s)	1498, 1798
AsuNHI	GCTAGC	1 cut(s)	3620
AxyI	CCTNAGG	1 cut(s)	2522
BaeGI	GKGCMC	1 cut(s)	3196
BaeI	ACNNNNGTAYC	2 cut(s)	1571, 1604
BalI	TGGCCA	4 cut(s)	333, 2358, 3567, 3984
BamHI	GGATCC	1 cut(s)	322
BanI	GGYRCC	2 cut(s)	1032, 2052
BanII	GRGCYC	4 cut(s)	176, 839, 985, 4103
BbsI	GAAGAC	1 cut(s)	825
Bbv12I	GWGCWC	6 cut(s)	176, 985, 2338, 2849, 3196, 3993
BbvI	GCAGC	9 cut(s)	510, 1607, 1906, 2285, 2392, 2641, 2959, 3154, 3652
BceAI	ACGGC	5 cut(s)	578, 2585, 4032, 4062, 4130
BciT130I	CCWGG	9 cut(s)	178, 506, 1424, 1668, 2340, 2355, 3140, 3373, 3728
BclI	TGATCA	1 cut(s)	1375
BcnI	CCSGG	3 cut(s)	320, 416, 1840
BcoDI	GTCTC	5 cut(s)	40, 1885, 2243, 3048, 4153
BfmI	CTRYAG	3 cut(s)	1875, 1884, 2574
BfoI	RGCGCY	1 cut(s)	2056
BfrI	CTTAAG	3 cut(s)	922, 1982, 2485
BfuAI	ACCTGC	1 cut(s)	176
BglII	AGATCT	2 cut(s)	3698, 3744
BlpI	GCTNAGC	1 cut(s)	3478
BmcAI	AGTACT	2 cut(s)	134, 586
BmrI	ACTGGG	3 cut(s)	3316, 3459, 4119
BmsI	GCATC	9 cut(s)	690, 1297, 1484, 1681, 2017, 2140, 2303, 2925, 3911
BmtI	GCTAGC	1 cut(s)	3624
BmuI	ACTGGG	3 cut(s)	3316, 3459, 4119
BpiI	GAAGAC	1 cut(s)	825
BplI	GAGNNNNNCTC	2 cut(s)	2672, 2704
BpmI	CTGGAG	4 cut(s)	160, 2219, 3072, 3394
Bpu10I	CCTNAGC	1 cut(s)	984
Bpu1102I	GCTNAGC	1 cut(s)	3478
Bpu14I	TTCGAA	2 cut(s)	1498, 1798
BpuEI	CTTGAG	4 cut(s)	1754, 2603, 4057, 4135
BpuMI	CCSGG	3 cut(s)	320, 416, 1840
BsaBI	GATNNNNATC	2 cut(s)	759, 3973
BsaHI	GRCGYC	2 cut(s)	468, 2053
BsaI	GGTCTC	2 cut(s)	2243, 3048
BsaJI	CCNNGG	8 cut(s)	462, 512, 689, 1422, 1667, 3138, 3925, 3979
BsaWI	WCCGGW	2 cut(s)	1647, 2122
BsaXI	ACNNNNNCTCC	4 cut(s)	2308, 2338, 3817, 3847
Bse118I	RCCGGY	1 cut(s)	362
Bse1I	ACTGG	8 cut(s)	1956, 2696, 2774, 3322, 3454, 3678, 3817, 4125
Bse21I	CCTNAGG	1 cut(s)	2522
Bse3DI	GCAATG	2 cut(s)	2062, 3472
Bse8I	GATNNNNATC	2 cut(s)	759, 3973
BseAI	TCCGGA	1 cut(s)	2122
BseBI	CCWGG	9 cut(s)	178, 506, 1424, 1668, 2340, 2355, 3140, 3373, 3728
BseDI	CCNNGG	8 cut(s)	462, 512, 689, 1422, 1667, 3138, 3925, 3979
BseGI	GGATG	8 cut(s)	379, 934, 1132, 1311, 1677, 1835, 2214, 3974
BseJI	GATNNNNATC	2 cut(s)	759, 3973
BseMI	GCAATG	2 cut(s)	2062, 3472
BseNI	ACTGG	8 cut(s)	1956, 2696, 2774, 3322, 3454, 3678, 3817, 4125
BseRI	GAGGAG	2 cut(s)	2542, 3930
BseSI	GKGCMC	1 cut(s)	3196
BseXI	GCAGC	9 cut(s)	510, 1607, 1906, 2285, 2392, 2641, 2959, 3154, 3652
BseYI	CCCAGC	1 cut(s)	2253
BsgI	GTGCAG	3 cut(s)	951, 2432, 2958
Bsh1236I	CGCG	1 cut(s)	1062
BshNI	GGYRCC	2 cut(s)	1032, 2052
BsiHKAI	GWGCWC	6 cut(s)	176, 985, 2338, 2849, 3196, 3993
BsiSI	CCGG	6 cut(s)	320, 363, 416, 1648, 1840, 2123
BslFI	GGGAC	3 cut(s)	174, 205, 4107
BsmAI	GTCTC	5 cut(s)	40, 1885, 2243, 3048, 4153
BsmBI	CGTCTC	1 cut(s)	4153
BsmFI	GGGAC	3 cut(s)	174, 205, 4107
BsmI	GAATGC	4 cut(s)	187, 2508, 2958, 3046
Bso31I	GGTCTC	2 cut(s)	2243, 3048
Bsp119I	TTCGAA	2 cut(s)	1498, 1798
Bsp1286I	GDGCHC	8 cut(s)	176, 839, 985, 2338, 2849, 3196, 3993, 4103
Bsp13I	TCCGGA	1 cut(s)	2122
Bsp1720I	GCTNAGC	1 cut(s)	3478
Bsp19I	CCATGG	2 cut(s)	512, 3979
BspACI	CCGC	8 cut(s)	216, 1324, 2018, 2408, 2540, 2862, 3871, 3911
BspEI	TCCGGA	1 cut(s)	2122
BspFNI	CGCG	1 cut(s)	1062
BspHI	TCATGA	2 cut(s)	3313, 3405
BspMI	ACCTGC	1 cut(s)	176
BspOI	GCTAGC	1 cut(s)	3624
BspQI	GCTCTTC	1 cut(s)	2724
BspT104I	TTCGAA	2 cut(s)	1498, 1798
BspT107I	GGYRCC	2 cut(s)	1032, 2052
BspTI	CTTAAG	3 cut(s)	922, 1982, 2485
BspTNI	GGTCTC	2 cut(s)	2243, 3048
BsrBI	CCGCTC	1 cut(s)	1324
BsrDI	GCAATG	2 cut(s)	2062, 3472
BsrFI	RCCGGY	1 cut(s)	362
BsrI	ACTGG	8 cut(s)	1956, 2696, 2774, 3322, 3454, 3678, 3817, 4125
BssAI	RCCGGY	1 cut(s)	362
BssECI	CCNNGG	8 cut(s)	462, 512, 689, 1422, 1667, 3138, 3925, 3979
BssNI	GRCGYC	2 cut(s)	468, 2053
BssT1I	CCWWGG	3 cut(s)	462, 512, 3979
Bst2UI	CCWGG	9 cut(s)	178, 506, 1424, 1668, 2340, 2355, 3140, 3373, 3728
Bst4CI	ACNGT	9 cut(s)	589, 788, 1319, 1879, 2578, 3059, 3106, 3178, 4118
Bst6I	CTCTTC	4 cut(s)	650, 2121, 2724, 3339
BstACI	GRCGYC	2 cut(s)	468, 2053
BstAFI	CTTAAG	3 cut(s)	922, 1982, 2485
BstAPI	GCANNNNNTGC	1 cut(s)	3908
BstBI	TTCGAA	2 cut(s)	1498, 1798
BstDSI	CCRYGG	3 cut(s)	512, 3925, 3979
BstEII	GGTNACC	1 cut(s)	2131
BstF5I	GGATG	8 cut(s)	379, 934, 1132, 1311, 1677, 1835, 2214, 3974
BstFNI	CGCG	1 cut(s)	1062
BstH2I	RGCGCY	1 cut(s)	2056
BstHHI	GCGC	2 cut(s)	2055, 2245
BstMAI	GTCTC	5 cut(s)	40, 1885, 2243, 3048, 4153
BstMWI	GCNNNNNNNGC	8 cut(s)	467, 1068, 2756, 2844, 3134, 3167, 3503, 3908
BstNI	CCWGG	9 cut(s)	178, 506, 1424, 1668, 2340, 2355, 3140, 3373, 3728
BstNSI	RCATGY	7 cut(s)	241, 632, 728, 1466, 1782, 1976, 3902
BstPI	GGTNACC	1 cut(s)	2131
BstSFI	CTRYAG	3 cut(s)	1875, 1884, 2574
BstSLI	GKGCMC	1 cut(s)	3196
BstUI	CGCG	1 cut(s)	1062
BstV1I	GCAGC	9 cut(s)	510, 1607, 1906, 2285, 2392, 2641, 2959, 3154, 3652
BstV2I	GAAGAC	1 cut(s)	825
BstX2I	RGATCY	7 cut(s)	201, 322, 574, 640, 1519, 3698, 3744
BstXI	CCANNNNNNTGG	2 cut(s)	123, 1466
BstYI	RGATCY	7 cut(s)	201, 322, 574, 640, 1519, 3698, 3744
Bsu36I	CCTNAGG	1 cut(s)	2522
BtgI	CCRYGG	3 cut(s)	512, 3925, 3979
BtsCI	GGATG	8 cut(s)	379, 934, 1132, 1311, 1677, 1835, 2214, 3974
BtsI	GCAGTG	2 cut(s)	248, 2967
BveI	ACCTGC	1 cut(s)	176
CaiI	CAGNNNCTG	3 cut(s)	2807, 2885, 3035
CciI	TCATGA	2 cut(s)	3313, 3405
CfoI	GCGC	2 cut(s)	2055, 2245
Cfr10I	RCCGGY	1 cut(s)	362
CseI	GACGC	2 cut(s)	476, 1051
CsiI	ACCWGGT	1 cut(s)	2338
DinI	GGCGCC	1 cut(s)	2054
DraI	TTTAAA	2 cut(s)	234, 2982
DraIII	CACNNNGTG	1 cut(s)	1757
EaeI	YGGCCR	5 cut(s)	331, 2356, 2748, 3565, 3982
Eam1104I	CTCTTC	4 cut(s)	650, 2121, 2724, 3339
EarI	CTCTTC	4 cut(s)	650, 2121, 2724, 3339
EciI	GGCGGA	1 cut(s)	2877
Ecl136II	GAGCTC	2 cut(s)	174, 983
Eco130I	CCWWGG	3 cut(s)	462, 512, 3979
Eco147I	AGGCCT	2 cut(s)	2684, 2854
Eco24I	GRGCYC	4 cut(s)	176, 839, 985, 4103
Eco31I	GGTCTC	2 cut(s)	2243, 3048
Eco32I	GATATC	2 cut(s)	732, 944
Eco53kI	GAGCTC	2 cut(s)	174, 983
Eco57I	CTGAAG	8 cut(s)	282, 366, 1358, 2775, 3183, 3261, 3636, 4092
Eco81I	CCTNAGG	1 cut(s)	2522
Eco91I	GGTNACC	1 cut(s)	2131
EcoICRI	GAGCTC	2 cut(s)	174, 983
EcoO109I	RGGNCCY	5 cut(s)	140, 188, 219, 869, 3369
EcoO65I	GGTNACC	1 cut(s)	2131
EcoRII	CCWGG	9 cut(s)	176, 504, 1422, 1666, 2338, 2353, 3138, 3371, 3726
EcoRV	GATATC	2 cut(s)	732, 944
EcoT14I	CCWWGG	3 cut(s)	462, 512, 3979
EcoT22I	ATGCAT	4 cut(s)	634, 1615, 2296, 3904
EcoT38I	GRGCYC	4 cut(s)	176, 839, 985, 4103
EgeI	GGCGCC	1 cut(s)	2054
EheI	GGCGCC	1 cut(s)	2054
ErhI	CCWWGG	3 cut(s)	462, 512, 3979
Esp3I	CGTCTC	1 cut(s)	4153
FalI	AAGNNNNNCTT	4 cut(s)	446, 478, 3058, 3090
FaqI	GGGAC	3 cut(s)	174, 205, 4107
FauI	CCCGC	2 cut(s)	209, 3878
FauNDI	CATATG	1 cut(s)	3790
FbaI	TGATCA	1 cut(s)	1375
FokI	GGATG	8 cut(s)	366, 921, 1139, 1298, 1684, 1822, 2201, 3961
FriOI	GRGCYC	4 cut(s)	176, 839, 985, 4103
GlaI	GCGC	2 cut(s)	2054, 2244
GsaI	CCCAGC	1 cut(s)	2257
GsuI	CTGGAG	4 cut(s)	160, 2219, 3072, 3394
HaeII	RGCGCY	1 cut(s)	2056
HapII	CCGG	6 cut(s)	320, 363, 416, 1648, 1840, 2123
HgaI	GACGC	2 cut(s)	476, 1051
HhaI	GCGC	2 cut(s)	2055, 2245
Hin1I	GRCGYC	2 cut(s)	468, 2053
Hin6I	GCGC	2 cut(s)	2053, 2243
HinP1I	GCGC	2 cut(s)	2053, 2243
HincII	GTYRAC	2 cut(s)	667, 2938
HindII	GTYRAC	2 cut(s)	667, 2938
HindIII	AAGCTT	2 cut(s)	455, 1696
HpaI	GTTAAC	1 cut(s)	667
HpaII	CCGG	6 cut(s)	320, 363, 416, 1648, 1840, 2123
Hpy166II	GTNNAC	7 cut(s)	667, 2938, 3194, 3445, 3586, 4013, 4130
Hpy188I	TCNGA	9 cut(s)	346, 679, 908, 1716, 1792, 1909, 2227, 2680, 3592
Hpy8I	GTNNAC	7 cut(s)	667, 2938, 3194, 3445, 3586, 4013, 4130
HpyAV	CCTTC	8 cut(s)	266, 1889, 2042, 2695, 2896, 3182, 3412, 3445
HpyCH4III	ACNGT	9 cut(s)	589, 788, 1319, 1879, 2578, 3059, 3106, 3178, 4118
HpyCH4IV	ACGT	6 cut(s)	445, 1210, 2475, 3294, 3441, 3532
HpyF10VI	GCNNNNNNNGC	8 cut(s)	467, 1068, 2756, 2844, 3134, 3167, 3503, 3908
HpySE526I	ACGT	6 cut(s)	445, 1210, 2475, 3294, 3441, 3532
Hsp92I	GRCGYC	2 cut(s)	468, 2053
HspAI	GCGC	2 cut(s)	2053, 2243
KasI	GGCGCC	1 cut(s)	2052
KflI	GGGWCCC	1 cut(s)	219
Kpn2I	TCCGGA	1 cut(s)	2122
KpnI	GGTACC	1 cut(s)	1036
Ksp22I	TGATCA	1 cut(s)	1375
KspAI	GTTAAC	1 cut(s)	667
LguI	GCTCTTC	1 cut(s)	2724
LmnI	GCTCC	9 cut(s)	171, 179, 418, 909, 1399, 2333, 2452, 2827, 3375
Lsp1109I	GCAGC	9 cut(s)	510, 1607, 1906, 2285, 2392, 2641, 2959, 3154, 3652
LweI	GCATC	9 cut(s)	690, 1297, 1484, 1681, 2017, 2140, 2303, 2925, 3911
MabI	ACCWGGT	1 cut(s)	2338
MaeII	ACGT	6 cut(s)	445, 1210, 2475, 3294, 3441, 3532
MaeIII	GTNAC	9 cut(s)	78, 432, 1000, 1063, 1828, 2131, 2881, 3381, 3929
MbiI	CCGCTC	1 cut(s)	1324
MfeI	CAATTG	3 cut(s)	1927, 2115, 2833
MflI	RGATCY	7 cut(s)	201, 322, 574, 640, 1519, 3698, 3744
MhlI	GDGCHC	8 cut(s)	176, 839, 985, 2338, 2849, 3196, 3993, 4103
MlsI	TGGCCA	4 cut(s)	333, 2358, 3567, 3984
MluNI	TGGCCA	4 cut(s)	333, 2358, 3567, 3984
Mly113I	GGCGCC	1 cut(s)	2053
MlyI	GAGTC	6 cut(s)	1193, 1987, 2374, 2594, 3581, 3926
MmeI	TCCRAC	4 cut(s)	1447, 1483, 1556, 2590
Mox20I	TGGCCA	4 cut(s)	333, 2358, 3567, 3984
Mph1103I	ATGCAT	4 cut(s)	634, 1615, 2296, 3904
MroI	TCCGGA	1 cut(s)	2122
MscI	TGGCCA	4 cut(s)	333, 2358, 3567, 3984
MslI	CAYNNNNRTG	8 cut(s)	89, 121, 480, 1406, 2067, 3404, 3573, 3996
Msp20I	TGGCCA	4 cut(s)	333, 2358, 3567, 3984
MspA1I	CMGCKG	4 cut(s)	329, 523, 2018, 3614
MspCI	CTTAAG	3 cut(s)	922, 1982, 2485
MspI	CCGG	6 cut(s)	320, 363, 416, 1648, 1840, 2123
MssI	GTTTAAAC	1 cut(s)	234
MunI	CAATTG	3 cut(s)	1927, 2115, 2833
Mva1269I	GAATGC	4 cut(s)	187, 2508, 2958, 3046
MvaI	CCWGG	9 cut(s)	178, 506, 1424, 1668, 2340, 2355, 3140, 3373, 3728
MvnI	CGCG	1 cut(s)	1062
MwoI	GCNNNNNNNGC	8 cut(s)	467, 1068, 2756, 2844, 3134, 3167, 3503, 3908
NarI	GGCGCC	1 cut(s)	2053
NciI	CCSGG	3 cut(s)	320, 416, 1840
NcoI	CCATGG	2 cut(s)	512, 3979
NdeI	CATATG	1 cut(s)	3790
NheI	GCTAGC	1 cut(s)	3620
NmuCI	GTSAC	6 cut(s)	78, 1000, 1063, 1828, 2881, 3929
NsiI	ATGCAT	4 cut(s)	634, 1615, 2296, 3904
NspI	RCATGY	7 cut(s)	241, 632, 728, 1466, 1782, 1976, 3902
NspV	TTCGAA	2 cut(s)	1498, 1798
OliI	CACNNNNGTG	1 cut(s)	89
PacI	TTAATTAA	1 cut(s)	2211
PaeI	GCATGC	2 cut(s)	728, 1976
PagI	TCATGA	2 cut(s)	3313, 3405
PceI	AGGCCT	2 cut(s)	2684, 2854
PciI	ACATGT	2 cut(s)	1462, 1778
PciSI	GCTCTTC	1 cut(s)	2724
PcsI	WCGNNNNNNNCGW	1 cut(s)	3922
PctI	GAATGC	4 cut(s)	187, 2508, 2958, 3046
PflMI	CCANNNNNTGG	2 cut(s)	310, 1538
PfoI	TCCNGGA	4 cut(s)	176, 414, 3371, 3726
PleI	GAGTC	6 cut(s)	1192, 1986, 2373, 2593, 3581, 3926
PluTI	GGCGCC	1 cut(s)	2056
PmeI	GTTTAAAC	1 cut(s)	234
PpsI	GAGTC	6 cut(s)	1192, 1986, 2373, 2593, 3581, 3926
PpuMI	RGGWCCY	5 cut(s)	140, 188, 219, 869, 3369
PscI	ACATGT	2 cut(s)	1462, 1778
PshBI	ATTAAT	1 cut(s)	2207
Psp124BI	GAGCTC	2 cut(s)	176, 985
Psp1406I	AACGTT	1 cut(s)	3294
Psp5II	RGGWCCY	5 cut(s)	140, 188, 219, 869, 3369
Psp6I	CCWGG	9 cut(s)	176, 504, 1422, 1666, 2338, 2353, 3138, 3371, 3726
PspEI	GGTNACC	1 cut(s)	2131
PspFI	CCCAGC	1 cut(s)	2253
PspGI	CCWGG	9 cut(s)	176, 504, 1422, 1666, 2338, 2353, 3138, 3371, 3726
PspPPI	RGGWCCY	5 cut(s)	140, 188, 219, 869, 3369
PsrI	GAACNNNNNNTAC	2 cut(s)	543, 575
PstNI	CAGNNNCTG	3 cut(s)	2807, 2885, 3035
PsuI	RGATCY	7 cut(s)	201, 322, 574, 640, 1519, 3698, 3744
PvuII	CAGCTG	3 cut(s)	329, 523, 3614
RseI	CAYNNNNRTG	8 cut(s)	89, 121, 480, 1406, 2067, 3404, 3573, 3996
SacI	GAGCTC	2 cut(s)	176, 985
SapI	GCTCTTC	1 cut(s)	2724
ScaI	AGTACT	2 cut(s)	134, 586
SchI	GAGTC	6 cut(s)	1193, 1987, 2374, 2594, 3581, 3926
SduI	GDGCHC	8 cut(s)	176, 839, 985, 2338, 2849, 3196, 3993, 4103
SexAI	ACCWGGT	1 cut(s)	2338
SfaNI	GCATC	9 cut(s)	690, 1297, 1484, 1681, 2017, 2140, 2303, 2925, 3911
SfcI	CTRYAG	3 cut(s)	1875, 1884, 2574
SfoI	GGCGCC	1 cut(s)	2054
SfuI	TTCGAA	2 cut(s)	1498, 1798
SmiMI	CAYNNNNRTG	8 cut(s)	89, 121, 480, 1406, 2067, 3404, 3573, 3996
SmlI	CTYRAG	7 cut(s)	922, 1733, 1982, 2485, 2582, 4072, 4150
SmoI	CTYRAG	7 cut(s)	922, 1733, 1982, 2485, 2582, 4072, 4150
SphI	GCATGC	2 cut(s)	728, 1976
SseBI	AGGCCT	2 cut(s)	2684, 2854
SsiI	CCGC	8 cut(s)	216, 1324, 2018, 2408, 2540, 2862, 3871, 3911
SspDI	GGCGCC	1 cut(s)	2052
SspI	AATATT	1 cut(s)	1818
SstI	GAGCTC	2 cut(s)	176, 985
StuI	AGGCCT	2 cut(s)	2684, 2854
StyI	CCWWGG	3 cut(s)	462, 512, 3979
TaaI	ACNGT	9 cut(s)	589, 788, 1319, 1879, 2578, 3059, 3106, 3178, 4118
TaiI	ACGT	6 cut(s)	448, 1213, 2478, 3297, 3444, 3535
TaqII	GACCGA	1 cut(s)	759
TatI	WGTACW	5 cut(s)	132, 584, 1131, 2493, 3178
TauI	GCSGC	1 cut(s)	3914
TseFI	GTSAC	6 cut(s)	78, 1000, 1063, 1828, 2881, 3929
TseI	GCWGC	9 cut(s)	523, 1595, 1919, 2273, 2405, 2629, 2972, 3167, 3640
Tsp45I	GTSAC	6 cut(s)	78, 1000, 1063, 1828, 2881, 3929
TspDTI	ATGAA	8 cut(s)	60, 482, 642, 1161, 1962, 2300, 3198, 3394
TspGWI	ACGGA	1 cut(s)	650
Van91I	CCANNNNNTGG	2 cut(s)	310, 1538
Vha464I	CTTAAG	3 cut(s)	922, 1982, 2485
VneI	GTGCAC	1 cut(s)	3192
VspI	ATTAAT	1 cut(s)	2207
XapI	RAATTY	6 cut(s)	826, 961, 1102, 1176, 1793, 3653
XceI	RCATGY	7 cut(s)	241, 632, 728, 1466, 1782, 1976, 3902
ZrmI	AGTACT	2 cut(s)	134, 586
Zsp2I	ATGCAT	4 cut(s)	634, 1615, 2296, 3904

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.