Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0008534

Physical Location & Seq

Genomic Coordinates Forward (+)

15628508 .. 15641801

Gene Structure 13294 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG148800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4146 bp

ATGGCGAAATCGAGACAACATTTCTCTAGCCAAGATTCATCGTTGTCACCCACTTCTGTGAGATCAAGAGAGTGGGAGGGTCCATCGAGATGGGCTGAGTACTTGGGTCCTGAACCAACTTCGCCCATGACATCTAGGAGCTCCAGGAATGCAGGTCCCGATGGACAGATCCAAAGTTCAGGCGGGTCCCACAAAGGTTTAAACATGCAGTGGGTAGTTCAACTAACTGAAGTAGCAGAAGGTCTTATGGCTAAAATGTATAGGTTAAACCAAACTTTGGATTACCCGGATCCAGCTGGCCATACATTTTCTGAAGCATTTTGGAAAGCCGGTGTGTTCCCCAACCATCCAAGACTTTGCTTGCTACTTTCAAAGAAGTTCCCGGAGCATTATAGCAAGTTACAGCTTGAACGTGTTGATAAGCTTGCCTTGGACGCCTTGCATGGTAATGCAGAGCTTCATTTACAAAGCCTGGAACCATGGGTTCAGCTGCTTCTTGACTTGATGGCATTTCGAGAACAAGCTCTACGGCTTATATTGGATCTAAGCAGTACTGTTATTACCTTGTTGCCTCACCAGAACTCGCTTATACTACATGCATTTATGGATCTCTTCTGTTCATTTGTCCGTGTTAACCTATTTTCCGAGAAGATACCGAGGAAAATGATGCTACAAATGTATAACCTTTTGCATGCGATATCAAGAAATGACCGAGATTGTGATTTCTATCATCGGTTGGTTCAATTTATAGACAGTTATGATCCACCCTTGAAAGGATTGAAAGAAGACCTGAATTTTGTGAGCCCCCGAATTGGAGAGGTGCTAGAGGCTGTGGGTCCTATTATTTTTCTATCAACAGATACAAGAAAGCTCCGAAATGAGGGATTCTTAAGTCCATATCATCCTCGATATCCAGACATACTTACAAATTCTGCACACCCAATGAGAGCTCAGGATTTAGCAAATGTCACCTCATATCGGGAATGGGTATTATTTGGGTACCTTGTTTGTCCAGATGAGCTTCTTCGCGTCACTAGCATTGATATTGCTCTGGTCGTCTTGAAAGAAAATTTAGTTCTTACATTGTTCAGGGATGAGTACATACTTTTGCATGAAGATTATCAGCTATTTGTCCTGCCTCGAATTTTAGAGTCAAAGAAGATGGCAAAATCTGGACGTACAAAGCAAAAAGAATCAGATTTGGAGTATAGTGTCGCAAAGCAAGTTGAGAAAATGATAAGTGAAGTGCATGAGCAAGCATTACTATCATGTGATGCCATCCACCGTGAGCGGAGGATATTACTGAAGCAAGAGATTGGAAGAATGGTACTTTTTTTCACTGATCAGCCTAGTTTGTTGGCTCCAAACATACAGATGGTATTCTCTGCCCTGGCTTTTGCTCAATGTGAAGTCCTCTGGTATTTCCAACATGTTGGAATTGCATCATCAAAATCTAAAGCTGTTCGAATGGTGTCAGTTGAAATAGATCCAAGTGATCCAACTATTGGGTTTTTGTTAGATGGAATGGACCATCTTTGCTGTTTGGTACGCAAGTATATTGCAGCAATTCGAGGTTATGCATTATCATACCTTTCTTCTTCTGCTGGTAGAATCCGGTTTTTGTTGAATACTCCTGGGATGGTGGCTCTTGACTTAGATGCAAGCTTGAAAAGTCTTTTTCAGAAGATTGTTCAGCACCTTGAGAACATACCAAAACCACAGGGTGAAAATGTATCTGCCATTACATGTGATCTATCTGAATTTCGAAAGGATTGGTTATCAATATTGATGATAGTCACATCATCCCGGTCATCTATAAACATAAGACATTTGGAGAAAGCTACAGTCTCTACAGGGAAAGAAGGCTTACTATCAGAGGGAAACGCTGCCTACAATTGGTCCAGATGTGTCGATGAACTGGAATCTCAATTATCAAAGCATGCGAGTCTTAAGAAGTTGTATTTCTACCATCAGCATCTTACAGCGGTATTCAGGAACACTATGTTTGGCCCAGAAGGGCGCCCACAACATTGCTGTGCATGGCTTGGTGTTGCAAGTAGTTTTCCAGAGTGTGCTTCGCCAATTGTTCCGGAAGAGGTAACCAAGATAGGCAGAGATGCAGTCCTTTATGTTGAATCTCTCATTGAATCTATAATGGGAGGGCTGGAGGGATTAATTAACATCCTAGATTCTGATGGAGGATTTGGTGCGCTAGAGACCCAGCTTCTTCCAGAGCAGGCAGCTTCTTATATGAACTATGCATCAAGAATGTCAATTACTTCAACTAAATCTCCAAAGGGAGCACCTGGTTTCCCTTTGCCTGGCCATGAGAGTCGTCCTGAAAATAATGCTTCTATCAAGATGTTAGAAGCTGCGGTGCAGAGATTGACCAACTTATGCTCAGTTTTGAATGATATGGAGCCTATATGTGTTCTAAACCACGTTTTTGTCTTAAGGGAGTACATGAGAGAGTGCATTCTTGGGAACCTTAGGAGGAGAGTGCTTGCGGTACTGAAAACTGACAATGACCTCCAACGGCCTACAGTTCTTGAGTCGCTTATTCGCAGACACATCAGCATTATCCATCTGGCAGAGCAGCATATTAGCATGGACTTAACTCAGGGTATTCGAGAAGTTTTGCTCTCAGAGGCCTTCTCAGGACCAGTTTCTTCTTTGCATTTGTTTGAAAAACCAGAAGAGCAACATACAGGATCGGCCACTGAAGCTGTCTGCAACTGGTACATTGAAAATATAATCAAGGATATATCAGGTGCTGGAATCCTTTTTGCTCCAATTCACAATTGCTTCAAGAGCACAAGGCCTGTTGGCGGATATTTTGCTGATTCAGTCACTGATCCAAGAGAATTGAAGGCATTTGCTCGCATCTTTGGTGGCTATGGAGTTGACAGGCTAGACAGAATGCTGAAAGAACACACTGCTGCACTTTTAAATTGTATTGACACATCACTGAGATCAAATCGTGATGTGCTAGAGGCAGTTGCTGGTAGCCTGCATTCTGGAGACCGTATAGAAAGAGAGGCTTTAATAAAGCAGATAGTCGATATAGACACGGTGATTGGGTTTTGTGTTCAAGCTGGCTTGGCCCTGGCTTTTGACAGACTTCTAGCTGAAGCTGCTGGCACTGTACTCTTTGAAGGTGCACCCTTGATACATTCATTATTAGCTGGAATATCTAAGCATATACCTGAAGAAATACCTGAAAAGAAAGAAGTCAGGAGATTGAGAGGGGTGGCAAACAACGTTGGTGTAGTCGATAATCATGATTCCCAGTGGGTGAGATTGATCTTAGAAGAGGTTGGGGGTGCAAATGATGGGTCCTGGAGCCTGTTACCCTATTTCTTTGCCACCTTCATGACATCTAATATATGGACTACCACAGCCTTCAACGTGGACACTGGGGGATTCAATAACAATATCCATTGCTTAGCAAGGTGTGTCAGTGCTGTAATTGCAGGAAGTGAGTTCGTTAGAGTGGAACGTGAATATCAGCAAAGACAATCTCTCTCAAATGGCCATGTTGGTGAATCAGTGGACTCAGAAATACAAAATCGTTTGTCAGCTGAAGCTAGCATAAAATCCACAATGCAGCTTTTCGTCAAATTCTCTGCAAGCATTATACTGGATTCTTGGAGTGAAACTAACAGATCTCATCTTGTAGCACAGCTAATTTTCCTGGACCAACTCTGCGAGATCTCACCTTATCTTCCAAGAAGCTCTCTGGAACCCCATGTTCCATATGCCATTCTTCGTTCAATATACAGCCAGTATTATACAAACTCTCCAAATACACCACTTGCATTACTAAGCATTTCACCCCGCCATTCGCCTGCTGTATCACTCTCACATGCATCTCCTGCGGCACGACAACCCCGTGGTGACTCCACTCCTCAGTATGATTCAGGTTATTTCAAAGGATCATCATCCCATGGCCAAGAGCACATTTATGATGGTGGAAGTTCACGAAGCACTGAAAACAGGCAACAGAACTTTCGCCGTTCTGGGCCTTTGGATTATGGCTCAAGCCGTAAAGTTAAGTTTGTGGAGGGCTCAACTTCAGGGAACAGTGGTCCCAGTCCACTACCAAGATTTGCCGTCTCAAGGTCGGGTCCAATATCGTACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006355 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008150 GO:0009653 GO:0009825 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010090 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010638 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0030029 GO:0030036 GO:0030154 GO:0030832 GO:0030833 GO:0030838 GO:0031209 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031984 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0032991 GO:0033043 GO:0040007 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045010 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051493 GO:0051495 GO:0060255 GO:0065007 GO:0065008 GO:0071840 GO:0080090 GO:0090066 GO:0090558 GO:0090626 GO:0097435 GO:0098827 GO:0110053 GO:1902680 GO:1902903 GO:1902905 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04810 map04810

Pfam Domains

Protein Families

Nckap1

Protein Analysis

1381

Amino Acids

154.36

Weight (kDa)

6.46

Isoelectric Point (pI)

48.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nckap1	PF09735	75 - 1265	0.0	Nck-associated protein 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008534)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35110 AT2G35110
fragaria_vesca	FvH4_3g12410 FvH4_3g12410
malus_domestica	MD05G1250500.v1.1 MD10G1231300.v1.1
prunus_persica	Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1
pyrus_communis	pycom05g22840 pycom10g19450
rosa_chinensis	RchiOBHm_Chr5g0020301
rosa_laevigata	RLG00000032516
rosa_multiflora	Rmu_sc0002087.1_g000004 Rmu_sc0007157.1_g000007
rosa_roxburghii	Rroxscaffold_1G00057910
rosa_rugosa	Rorug05G0058400
rosa_samantha	Rh5AG148800 Rh5BG148400 Rh5CG159900 Rh5DG147200
rosa_wichuraiana	Rw5G013180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	143
Acc65I	GGTACC	1 cut(s)	999
AccB1I	GGYRCC	2 cut(s)	999, 2019
AccB7I	CCANNNNNTGG	2 cut(s)	277, 1505
AccBSI	CCGCTC	1 cut(s)	1291
AccII	CGCG	1 cut(s)	1029
AccIII	TCCGGA	1 cut(s)	2089
AciI	CCGC	8 cut(s)	183, 1291, 1985, 2375, 2507, 2829, 3838, 3878
AclI	AACGTT	1 cut(s)	3261
AcoI	YGGCCR	5 cut(s)	298, 2323, 2715, 3532, 3949
AcsI	RAATTY	6 cut(s)	793, 928, 1069, 1143, 1760, 3620
AcuI	CTGAAG	8 cut(s)	249, 333, 1325, 2742, 3150, 3228, 3603, 4059
AcyI	GRCGYC	2 cut(s)	435, 2020
AdeI	CACNNNGTG	1 cut(s)	1724
AflII	CTTAAG	3 cut(s)	889, 1949, 2452
AflIII	ACRYGT	3 cut(s)	412, 1429, 1745
AjnI	CCWGG	9 cut(s)	143, 471, 1389, 1633, 2305, 2320, 3105, 3338, 3693
AleI	CACNNNNGTG	1 cut(s)	56
Alw21I	GWGCWC	6 cut(s)	143, 952, 2305, 2816, 3163, 3960
Alw26I	GTCTC	5 cut(s)	7, 1852, 2210, 3015, 4120
Alw44I	GTGCAC	1 cut(s)	3159
AlwNI	CAGNNNCTG	3 cut(s)	2774, 2852, 3002
Aor13HI	TCCGGA	1 cut(s)	2089
ApaLI	GTGCAC	1 cut(s)	3159
ApeKI	GCWGC	9 cut(s)	490, 1562, 1886, 2240, 2372, 2596, 2939, 3134, 3607
ApoI	RAATTY	6 cut(s)	793, 928, 1069, 1143, 1760, 3620
ArsI	GACNNNNNNTTYG	4 cut(s)	779, 811, 3320, 3352
AseI	ATTAAT	1 cut(s)	2174
Asp718I	GGTACC	1 cut(s)	999
AspLEI	GCGC	2 cut(s)	2022, 2212
AsuC2I	CCSGG	3 cut(s)	287, 383, 1807
AsuII	TTCGAA	2 cut(s)	1465, 1765
AsuNHI	GCTAGC	1 cut(s)	3587
AxyI	CCTNAGG	1 cut(s)	2489
BaeGI	GKGCMC	1 cut(s)	3163
BaeI	ACNNNNGTAYC	2 cut(s)	1538, 1571
BalI	TGGCCA	4 cut(s)	300, 2325, 3534, 3951
BamHI	GGATCC	1 cut(s)	289
BanI	GGYRCC	2 cut(s)	999, 2019
BanII	GRGCYC	4 cut(s)	143, 806, 952, 4070
BbsI	GAAGAC	1 cut(s)	792
Bbv12I	GWGCWC	6 cut(s)	143, 952, 2305, 2816, 3163, 3960
BbvI	GCAGC	9 cut(s)	477, 1574, 1873, 2252, 2359, 2608, 2926, 3121, 3619
BceAI	ACGGC	5 cut(s)	545, 2552, 3999, 4029, 4097
BciT130I	CCWGG	9 cut(s)	145, 473, 1391, 1635, 2307, 2322, 3107, 3340, 3695
BclI	TGATCA	1 cut(s)	1342
BcnI	CCSGG	3 cut(s)	287, 383, 1807
BcoDI	GTCTC	5 cut(s)	7, 1852, 2210, 3015, 4120
BfmI	CTRYAG	3 cut(s)	1842, 1851, 2541
BfoI	RGCGCY	1 cut(s)	2023
BfrI	CTTAAG	3 cut(s)	889, 1949, 2452
BfuAI	ACCTGC	1 cut(s)	143
BglII	AGATCT	2 cut(s)	3665, 3711
BlpI	GCTNAGC	1 cut(s)	3445
BmcAI	AGTACT	2 cut(s)	101, 553
BmrI	ACTGGG	3 cut(s)	3283, 3426, 4086
BmsI	GCATC	9 cut(s)	657, 1264, 1451, 1648, 1984, 2107, 2270, 2892, 3878
BmtI	GCTAGC	1 cut(s)	3591
BmuI	ACTGGG	3 cut(s)	3283, 3426, 4086
BpiI	GAAGAC	1 cut(s)	792
BplI	GAGNNNNNCTC	2 cut(s)	2639, 2671
BpmI	CTGGAG	4 cut(s)	127, 2186, 3039, 3361
Bpu10I	CCTNAGC	1 cut(s)	951
Bpu1102I	GCTNAGC	1 cut(s)	3445
Bpu14I	TTCGAA	2 cut(s)	1465, 1765
BpuEI	CTTGAG	4 cut(s)	1721, 2570, 4024, 4102
BpuMI	CCSGG	3 cut(s)	287, 383, 1807
BsaBI	GATNNNNATC	2 cut(s)	726, 3940
BsaHI	GRCGYC	2 cut(s)	435, 2020
BsaI	GGTCTC	2 cut(s)	2210, 3015
BsaJI	CCNNGG	8 cut(s)	429, 479, 656, 1389, 1634, 3105, 3892, 3946
BsaWI	WCCGGW	2 cut(s)	1614, 2089
BsaXI	ACNNNNNCTCC	4 cut(s)	2275, 2305, 3784, 3814
Bse118I	RCCGGY	1 cut(s)	329
Bse1I	ACTGG	8 cut(s)	1923, 2663, 2741, 3289, 3421, 3645, 3784, 4092
Bse21I	CCTNAGG	1 cut(s)	2489
Bse3DI	GCAATG	2 cut(s)	2029, 3439
Bse8I	GATNNNNATC	2 cut(s)	726, 3940
BseAI	TCCGGA	1 cut(s)	2089
BseBI	CCWGG	9 cut(s)	145, 473, 1391, 1635, 2307, 2322, 3107, 3340, 3695
BseDI	CCNNGG	8 cut(s)	429, 479, 656, 1389, 1634, 3105, 3892, 3946
BseGI	GGATG	8 cut(s)	346, 901, 1099, 1278, 1644, 1802, 2181, 3941
BseJI	GATNNNNATC	2 cut(s)	726, 3940
BseMI	GCAATG	2 cut(s)	2029, 3439
BseMII	CTCAG	9 cut(s)	87, 965, 2415, 2633, 2658, 2670, 2960, 3570, 3923
BseNI	ACTGG	8 cut(s)	1923, 2663, 2741, 3289, 3421, 3645, 3784, 4092
BseRI	GAGGAG	2 cut(s)	2509, 3897
BseSI	GKGCMC	1 cut(s)	3163
BseXI	GCAGC	9 cut(s)	477, 1574, 1873, 2252, 2359, 2608, 2926, 3121, 3619
BseYI	CCCAGC	1 cut(s)	2220
BsgI	GTGCAG	3 cut(s)	918, 2399, 2925
Bsh1236I	CGCG	1 cut(s)	1029
BshNI	GGYRCC	2 cut(s)	999, 2019
BsiHKAI	GWGCWC	6 cut(s)	143, 952, 2305, 2816, 3163, 3960
BsiSI	CCGG	6 cut(s)	287, 330, 383, 1615, 1807, 2090
BslFI	GGGAC	3 cut(s)	141, 172, 4074
BsmAI	GTCTC	5 cut(s)	7, 1852, 2210, 3015, 4120
BsmBI	CGTCTC	1 cut(s)	4120
BsmFI	GGGAC	3 cut(s)	141, 172, 4074
BsmI	GAATGC	4 cut(s)	154, 2475, 2925, 3013
Bso31I	GGTCTC	2 cut(s)	2210, 3015
Bsp119I	TTCGAA	2 cut(s)	1465, 1765
Bsp1286I	GDGCHC	8 cut(s)	143, 806, 952, 2305, 2816, 3163, 3960, 4070
Bsp13I	TCCGGA	1 cut(s)	2089
Bsp1720I	GCTNAGC	1 cut(s)	3445
Bsp19I	CCATGG	2 cut(s)	479, 3946
BspACI	CCGC	8 cut(s)	183, 1291, 1985, 2375, 2507, 2829, 3838, 3878
BspCNI	CTCAG	9 cut(s)	88, 964, 2414, 2632, 2657, 2669, 2961, 3569, 3922
BspEI	TCCGGA	1 cut(s)	2089
BspFNI	CGCG	1 cut(s)	1029
BspHI	TCATGA	2 cut(s)	3280, 3372
BspMI	ACCTGC	1 cut(s)	143
BspOI	GCTAGC	1 cut(s)	3591
BspQI	GCTCTTC	1 cut(s)	2691
BspT104I	TTCGAA	2 cut(s)	1465, 1765
BspT107I	GGYRCC	2 cut(s)	999, 2019
BspTI	CTTAAG	3 cut(s)	889, 1949, 2452
BspTNI	GGTCTC	2 cut(s)	2210, 3015
BsrBI	CCGCTC	1 cut(s)	1291
BsrDI	GCAATG	2 cut(s)	2029, 3439
BsrFI	RCCGGY	1 cut(s)	329
BsrI	ACTGG	8 cut(s)	1923, 2663, 2741, 3289, 3421, 3645, 3784, 4092
BssAI	RCCGGY	1 cut(s)	329
BssECI	CCNNGG	8 cut(s)	429, 479, 656, 1389, 1634, 3105, 3892, 3946
BssNI	GRCGYC	2 cut(s)	435, 2020
BssT1I	CCWWGG	3 cut(s)	429, 479, 3946
Bst2UI	CCWGG	9 cut(s)	145, 473, 1391, 1635, 2307, 2322, 3107, 3340, 3695
Bst4CI	ACNGT	9 cut(s)	556, 755, 1286, 1846, 2545, 3026, 3073, 3145, 4085
Bst6I	CTCTTC	4 cut(s)	617, 2088, 2691, 3306
BstACI	GRCGYC	2 cut(s)	435, 2020
BstAFI	CTTAAG	3 cut(s)	889, 1949, 2452
BstAPI	GCANNNNNTGC	1 cut(s)	3875
BstBI	TTCGAA	2 cut(s)	1465, 1765
BstDSI	CCRYGG	3 cut(s)	479, 3892, 3946
BstEII	GGTNACC	1 cut(s)	2098
BstF5I	GGATG	8 cut(s)	346, 901, 1099, 1278, 1644, 1802, 2181, 3941
BstFNI	CGCG	1 cut(s)	1029
BstH2I	RGCGCY	1 cut(s)	2023
BstHHI	GCGC	2 cut(s)	2022, 2212
BstMAI	GTCTC	5 cut(s)	7, 1852, 2210, 3015, 4120
BstMWI	GCNNNNNNNGC	8 cut(s)	434, 1035, 2723, 2811, 3101, 3134, 3470, 3875
BstNI	CCWGG	9 cut(s)	145, 473, 1391, 1635, 2307, 2322, 3107, 3340, 3695
BstNSI	RCATGY	7 cut(s)	208, 599, 695, 1433, 1749, 1943, 3869
BstPI	GGTNACC	1 cut(s)	2098
BstSFI	CTRYAG	3 cut(s)	1842, 1851, 2541
BstSLI	GKGCMC	1 cut(s)	3163
BstUI	CGCG	1 cut(s)	1029
BstV1I	GCAGC	9 cut(s)	477, 1574, 1873, 2252, 2359, 2608, 2926, 3121, 3619
BstV2I	GAAGAC	1 cut(s)	792
BstX2I	RGATCY	7 cut(s)	168, 289, 541, 607, 1486, 3665, 3711
BstXI	CCANNNNNNTGG	2 cut(s)	90, 1433
BstYI	RGATCY	7 cut(s)	168, 289, 541, 607, 1486, 3665, 3711
Bsu36I	CCTNAGG	1 cut(s)	2489
BtgI	CCRYGG	3 cut(s)	479, 3892, 3946
BtsCI	GGATG	8 cut(s)	346, 901, 1099, 1278, 1644, 1802, 2181, 3941
BtsI	GCAGTG	2 cut(s)	215, 2934
BveI	ACCTGC	1 cut(s)	143
CaiI	CAGNNNCTG	3 cut(s)	2774, 2852, 3002
CciI	TCATGA	2 cut(s)	3280, 3372
CfoI	GCGC	2 cut(s)	2022, 2212
Cfr10I	RCCGGY	1 cut(s)	329
CseI	GACGC	2 cut(s)	443, 1018
CsiI	ACCWGGT	1 cut(s)	2305
DinI	GGCGCC	1 cut(s)	2021
DraI	TTTAAA	2 cut(s)	201, 2949
DraIII	CACNNNGTG	1 cut(s)	1724
EaeI	YGGCCR	5 cut(s)	298, 2323, 2715, 3532, 3949
Eam1104I	CTCTTC	4 cut(s)	617, 2088, 2691, 3306
EarI	CTCTTC	4 cut(s)	617, 2088, 2691, 3306
EciI	GGCGGA	1 cut(s)	2844
Ecl136II	GAGCTC	2 cut(s)	141, 950
Eco130I	CCWWGG	3 cut(s)	429, 479, 3946
Eco147I	AGGCCT	2 cut(s)	2651, 2821
Eco24I	GRGCYC	4 cut(s)	143, 806, 952, 4070
Eco31I	GGTCTC	2 cut(s)	2210, 3015
Eco32I	GATATC	2 cut(s)	699, 911
Eco53kI	GAGCTC	2 cut(s)	141, 950
Eco57I	CTGAAG	8 cut(s)	249, 333, 1325, 2742, 3150, 3228, 3603, 4059
Eco81I	CCTNAGG	1 cut(s)	2489
Eco91I	GGTNACC	1 cut(s)	2098
EcoICRI	GAGCTC	2 cut(s)	141, 950
EcoO109I	RGGNCCY	5 cut(s)	107, 155, 186, 836, 3336
EcoO65I	GGTNACC	1 cut(s)	2098
EcoRII	CCWGG	9 cut(s)	143, 471, 1389, 1633, 2305, 2320, 3105, 3338, 3693
EcoRV	GATATC	2 cut(s)	699, 911
EcoT14I	CCWWGG	3 cut(s)	429, 479, 3946
EcoT22I	ATGCAT	4 cut(s)	601, 1582, 2263, 3871
EcoT38I	GRGCYC	4 cut(s)	143, 806, 952, 4070
EgeI	GGCGCC	1 cut(s)	2021
EheI	GGCGCC	1 cut(s)	2021
ErhI	CCWWGG	3 cut(s)	429, 479, 3946
Esp3I	CGTCTC	1 cut(s)	4120
FalI	AAGNNNNNCTT	4 cut(s)	413, 445, 3025, 3057
FaqI	GGGAC	3 cut(s)	141, 172, 4074
FauI	CCCGC	2 cut(s)	176, 3845
FauNDI	CATATG	1 cut(s)	3757
FbaI	TGATCA	1 cut(s)	1342
FokI	GGATG	8 cut(s)	333, 888, 1106, 1265, 1651, 1789, 2168, 3928
FriOI	GRGCYC	4 cut(s)	143, 806, 952, 4070
GlaI	GCGC	2 cut(s)	2021, 2211
GsaI	CCCAGC	1 cut(s)	2224
GsuI	CTGGAG	4 cut(s)	127, 2186, 3039, 3361
HaeII	RGCGCY	1 cut(s)	2023
HapII	CCGG	6 cut(s)	287, 330, 383, 1615, 1807, 2090
HgaI	GACGC	2 cut(s)	443, 1018
HhaI	GCGC	2 cut(s)	2022, 2212
Hin1I	GRCGYC	2 cut(s)	435, 2020
Hin6I	GCGC	2 cut(s)	2020, 2210
HinP1I	GCGC	2 cut(s)	2020, 2210
HincII	GTYRAC	2 cut(s)	634, 2905
HindII	GTYRAC	2 cut(s)	634, 2905
HindIII	AAGCTT	2 cut(s)	422, 1663
HpaI	GTTAAC	1 cut(s)	634
HpaII	CCGG	6 cut(s)	287, 330, 383, 1615, 1807, 2090
Hpy166II	GTNNAC	7 cut(s)	634, 2905, 3161, 3412, 3553, 3980, 4097
Hpy8I	GTNNAC	7 cut(s)	634, 2905, 3161, 3412, 3553, 3980, 4097
HpyAV	CCTTC	8 cut(s)	233, 1856, 2009, 2662, 2863, 3149, 3379, 3412
HpyCH4III	ACNGT	9 cut(s)	556, 755, 1286, 1846, 2545, 3026, 3073, 3145, 4085
HpyCH4IV	ACGT	6 cut(s)	412, 1177, 2442, 3261, 3408, 3499
HpyF10VI	GCNNNNNNNGC	8 cut(s)	434, 1035, 2723, 2811, 3101, 3134, 3470, 3875
HpySE526I	ACGT	6 cut(s)	412, 1177, 2442, 3261, 3408, 3499
Hsp92I	GRCGYC	2 cut(s)	435, 2020
HspAI	GCGC	2 cut(s)	2020, 2210
KasI	GGCGCC	1 cut(s)	2019
KflI	GGGWCCC	1 cut(s)	186
Kpn2I	TCCGGA	1 cut(s)	2089
KpnI	GGTACC	1 cut(s)	1003
Ksp22I	TGATCA	1 cut(s)	1342
KspAI	GTTAAC	1 cut(s)	634
LguI	GCTCTTC	1 cut(s)	2691
LmnI	GCTCC	9 cut(s)	138, 146, 385, 876, 1366, 2300, 2419, 2794, 3342
Lsp1109I	GCAGC	9 cut(s)	477, 1574, 1873, 2252, 2359, 2608, 2926, 3121, 3619
LweI	GCATC	9 cut(s)	657, 1264, 1451, 1648, 1984, 2107, 2270, 2892, 3878
MabI	ACCWGGT	1 cut(s)	2305
MaeII	ACGT	6 cut(s)	412, 1177, 2442, 3261, 3408, 3499
MaeIII	GTNAC	9 cut(s)	45, 399, 967, 1030, 1795, 2098, 2848, 3348, 3896
MbiI	CCGCTC	1 cut(s)	1291
MfeI	CAATTG	3 cut(s)	1894, 2082, 2800
MflI	RGATCY	7 cut(s)	168, 289, 541, 607, 1486, 3665, 3711
MhlI	GDGCHC	8 cut(s)	143, 806, 952, 2305, 2816, 3163, 3960, 4070
MlsI	TGGCCA	4 cut(s)	300, 2325, 3534, 3951
MluNI	TGGCCA	4 cut(s)	300, 2325, 3534, 3951
Mly113I	GGCGCC	1 cut(s)	2020
MlyI	GAGTC	6 cut(s)	1160, 1954, 2341, 2561, 3548, 3893
MmeI	TCCRAC	4 cut(s)	1414, 1450, 1523, 2557
Mox20I	TGGCCA	4 cut(s)	300, 2325, 3534, 3951
Mph1103I	ATGCAT	4 cut(s)	601, 1582, 2263, 3871
MroI	TCCGGA	1 cut(s)	2089
MscI	TGGCCA	4 cut(s)	300, 2325, 3534, 3951
MslI	CAYNNNNRTG	8 cut(s)	56, 88, 447, 1373, 2034, 3371, 3540, 3963
Msp20I	TGGCCA	4 cut(s)	300, 2325, 3534, 3951
MspA1I	CMGCKG	4 cut(s)	296, 490, 1985, 3581
MspCI	CTTAAG	3 cut(s)	889, 1949, 2452
MspI	CCGG	6 cut(s)	287, 330, 383, 1615, 1807, 2090
MssI	GTTTAAAC	1 cut(s)	201
MunI	CAATTG	3 cut(s)	1894, 2082, 2800
Mva1269I	GAATGC	4 cut(s)	154, 2475, 2925, 3013
MvaI	CCWGG	9 cut(s)	145, 473, 1391, 1635, 2307, 2322, 3107, 3340, 3695
MvnI	CGCG	1 cut(s)	1029
MwoI	GCNNNNNNNGC	8 cut(s)	434, 1035, 2723, 2811, 3101, 3134, 3470, 3875
NarI	GGCGCC	1 cut(s)	2020
NciI	CCSGG	3 cut(s)	287, 383, 1807
NcoI	CCATGG	2 cut(s)	479, 3946
NdeI	CATATG	1 cut(s)	3757
NheI	GCTAGC	1 cut(s)	3587
NmuCI	GTSAC	6 cut(s)	45, 967, 1030, 1795, 2848, 3896
NsiI	ATGCAT	4 cut(s)	601, 1582, 2263, 3871
NspI	RCATGY	7 cut(s)	208, 599, 695, 1433, 1749, 1943, 3869
NspV	TTCGAA	2 cut(s)	1465, 1765
OliI	CACNNNNGTG	1 cut(s)	56
PacI	TTAATTAA	1 cut(s)	2178
PaeI	GCATGC	2 cut(s)	695, 1943
PagI	TCATGA	2 cut(s)	3280, 3372
PceI	AGGCCT	2 cut(s)	2651, 2821
PciI	ACATGT	2 cut(s)	1429, 1745
PciSI	GCTCTTC	1 cut(s)	2691
PcsI	WCGNNNNNNNCGW	1 cut(s)	3889
PctI	GAATGC	4 cut(s)	154, 2475, 2925, 3013
PflMI	CCANNNNNTGG	2 cut(s)	277, 1505
PfoI	TCCNGGA	4 cut(s)	143, 381, 3338, 3693
PleI	GAGTC	6 cut(s)	1159, 1953, 2340, 2560, 3548, 3893
PluTI	GGCGCC	1 cut(s)	2023
PmeI	GTTTAAAC	1 cut(s)	201
PpsI	GAGTC	6 cut(s)	1159, 1953, 2340, 2560, 3548, 3893
PpuMI	RGGWCCY	5 cut(s)	107, 155, 186, 836, 3336
PscI	ACATGT	2 cut(s)	1429, 1745
PshBI	ATTAAT	1 cut(s)	2174
Psp124BI	GAGCTC	2 cut(s)	143, 952
Psp1406I	AACGTT	1 cut(s)	3261
Psp5II	RGGWCCY	5 cut(s)	107, 155, 186, 836, 3336
Psp6I	CCWGG	9 cut(s)	143, 471, 1389, 1633, 2305, 2320, 3105, 3338, 3693
PspEI	GGTNACC	1 cut(s)	2098
PspFI	CCCAGC	1 cut(s)	2220
PspGI	CCWGG	9 cut(s)	143, 471, 1389, 1633, 2305, 2320, 3105, 3338, 3693
PspPPI	RGGWCCY	5 cut(s)	107, 155, 186, 836, 3336
PsrI	GAACNNNNNNTAC	2 cut(s)	510, 542
PstNI	CAGNNNCTG	3 cut(s)	2774, 2852, 3002
PsuI	RGATCY	7 cut(s)	168, 289, 541, 607, 1486, 3665, 3711
PvuII	CAGCTG	3 cut(s)	296, 490, 3581
RseI	CAYNNNNRTG	8 cut(s)	56, 88, 447, 1373, 2034, 3371, 3540, 3963
SacI	GAGCTC	2 cut(s)	143, 952
SapI	GCTCTTC	1 cut(s)	2691
ScaI	AGTACT	2 cut(s)	101, 553
SchI	GAGTC	6 cut(s)	1160, 1954, 2341, 2561, 3548, 3893
SduI	GDGCHC	8 cut(s)	143, 806, 952, 2305, 2816, 3163, 3960, 4070
SexAI	ACCWGGT	1 cut(s)	2305
SfaNI	GCATC	9 cut(s)	657, 1264, 1451, 1648, 1984, 2107, 2270, 2892, 3878
SfcI	CTRYAG	3 cut(s)	1842, 1851, 2541
SfoI	GGCGCC	1 cut(s)	2021
SfuI	TTCGAA	2 cut(s)	1465, 1765
SmiMI	CAYNNNNRTG	8 cut(s)	56, 88, 447, 1373, 2034, 3371, 3540, 3963
SmlI	CTYRAG	7 cut(s)	889, 1700, 1949, 2452, 2549, 4039, 4117
SmoI	CTYRAG	7 cut(s)	889, 1700, 1949, 2452, 2549, 4039, 4117
SphI	GCATGC	2 cut(s)	695, 1943
SseBI	AGGCCT	2 cut(s)	2651, 2821
SsiI	CCGC	8 cut(s)	183, 1291, 1985, 2375, 2507, 2829, 3838, 3878
SspDI	GGCGCC	1 cut(s)	2019
SspI	AATATT	1 cut(s)	1785
SstI	GAGCTC	2 cut(s)	143, 952
StuI	AGGCCT	2 cut(s)	2651, 2821
StyI	CCWWGG	3 cut(s)	429, 479, 3946
TaaI	ACNGT	9 cut(s)	556, 755, 1286, 1846, 2545, 3026, 3073, 3145, 4085
TaiI	ACGT	6 cut(s)	415, 1180, 2445, 3264, 3411, 3502
TaqII	GACCGA	1 cut(s)	726
TatI	WGTACW	5 cut(s)	99, 551, 1098, 2460, 3145
TauI	GCSGC	1 cut(s)	3881
TseFI	GTSAC	6 cut(s)	45, 967, 1030, 1795, 2848, 3896
TseI	GCWGC	9 cut(s)	490, 1562, 1886, 2240, 2372, 2596, 2939, 3134, 3607
Tsp45I	GTSAC	6 cut(s)	45, 967, 1030, 1795, 2848, 3896
TspDTI	ATGAA	8 cut(s)	27, 449, 609, 1128, 1929, 2267, 3165, 3361
TspGWI	ACGGA	1 cut(s)	617
Van91I	CCANNNNNTGG	2 cut(s)	277, 1505
Vha464I	CTTAAG	3 cut(s)	889, 1949, 2452
VneI	GTGCAC	1 cut(s)	3159
VspI	ATTAAT	1 cut(s)	2174
XapI	RAATTY	6 cut(s)	793, 928, 1069, 1143, 1760, 3620
XceI	RCATGY	7 cut(s)	208, 599, 695, 1433, 1749, 1943, 3869
ZrmI	AGTACT	2 cut(s)	101, 553
Zsp2I	ATGCAT	4 cut(s)	601, 1582, 2263, 3871

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.