Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008534

Physical Location & Seq

Genomic Coordinates Reverse (-)

32799564 .. 32810831

Gene Structure 11268 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1231300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4146 bp

ATGGCAAAATCGAGACAACATTTCTCTAGCCAAGATTCATCACTGTCACCAACTTCTGTGCGATCACGAGAATGGGAGGGTCCATCAAGATGGACTGAGTACTTGGGTCCAGAGACAACTTCTCCAATGAATTTGAGGAGCTCCAGGAATTCTGGTCCTGATGGACAGGTCCATAGTTCAGGCGGCTCCCACAAAGGCTTGAACATGCAGTGGGTAGTTCAACTAACTGAAGTGGCAGAAGGCCTTATGGCTAAAATGTATAGGCTGAACCAAATTTTGGATTACCCGGATCCAGTTGGCCATGTATTTTCAGAGGCATTTTGGAAAGCTGGTGTGTTCCCCAACCATCCAAGAACTTGCTTGTTAGTCTCAAAGAAGTTCCCTGAACATTATAGCAAGTTACAGCTCGAACGTATTGATAAGGTTTCTTGGGATGCCTTGCAAGATAATGCAGAACTTCATTTACAAAGCTTAGAGCCATGGATCCAGCTGCTTTTTGATTTGATGGCCTTCCGAGAACAAGCTCTACGACTTATATTGGATCTGAGCAGTACAGTTATTACCTTGTTGCCCCATCAGAACTCACTTATACTACATGCATTTATGGATCTCTTTTGTTCCTTTGTACGCGTTAACCTTTTCTCGGAGAAGATACCAAGGAAAATGATGCTACAAATGTATAACCTTTTGCATTCCATGTCAAGAGACCGAGATTGTGATTTTTATCATCGGTTGGTTCAGTTTATAGACAGTTATGATCCACCGTTGAAAGGATTAAAAGAAGACCTAAATTTTGTGAGCCCACGTATTGGAGAGGTGCTAGAGGCTGTGGGACCTATTATTTTTTTGTCAACAGATACAAGAAAGCTCAGAAATGAAGGTTTTTTAAGTCCATATCATCCTCGATATCCAGACATACTTACAAATTCTGCTCATCCAATGAGAGCTCAGGATCTAGCAAATGTCACCTCTTATCGTGAATGGGTATTATTTGGGTATCTTGTTTGTCCTGATGAGCTTCTTCGCGTCACTAGCATTGATATTGCTTTGGTCGTATTGAAAGAAAATCTGGTTCTTACATTGTTCAGGGATGAGTATATACTTTTGCATGAGGATTATCAGCTATATGTCCTGCCACGAATTTTAGAATCAAAGAAGATGGCAAAGTCTGGTCGTACGAAGCAAAAAGAAGCAGATCTGGAGTATAGTGTAGCAAAGCAAGTCGAGAAAATGATAAGTGAAGTGCATGAGCAAGCATTACTCTCATGTGATGCCATTCATCGTGAGAGGAGAATCTTGCTAAAGCAAGAGATTGGAAGAATGGCACTTTTCTTCACTGATCAGCCTAGTTTGTTGGCTCCAAACATACAGATGGTATTCTCTGCCTTGGCTTTTGCTCAATGTGAGGTCATCTGGTATTTCCAACATGTTGGGATTGCATCATCAAAATCTAAAACCACTCGCATGGTACCTGTTGACATTGACCCAAGTGATCCAACTATCGGGTTCTTATTAGATGGAATGGACCATCTGTGCTGTTTAGTACGCAAGTACATTGCAGCGATTCGAGGTTATGCATTATCATACCTTTCTTCTTGTGCTGTTAGAATCCGGTCTTTGTTGAATACCCCTGGGATGGTGGCTCTTGACTTAGATTCAAGCTTGAAAGGTCTTTTTCAGCAGATTGTTCAGCACCTTGAAAACATACCAAAACCGCAGGGTGAAAATGTATCTGCCATTACATGTGATCTAACTGAATTTCGAAGGGATTGGTTATCAATATTGATGATAGTCACATCTTCCCGGTCATCTATAAACATACGACATTTGGAGAAAGCTACAGTTTCTACTGGGAAAGAAGGCTTATTATCGGAAGGAAATGCTGCATACAATTGGTCCAGATGTGTTGACGAACTGGAAACTCAATTATCAGAGCATGCGAGTCTTAAGAAACTGTATTTCTACCATCAGCACCTCACAGCAGTCTTCAGGAATACTATGTTTGGCCCAGAAGGTCGCCCACAGCATTGCTGTGCATGGCTTGGCGTTGCAAGTAGTTTTCCAGAATGTGCTTCCCCAATTGTTCCAGAAGAGGTAACTAAAATTGGCAGGGATGCAGTCCTTTATGTTGAATCTCTGATCGAATCAATAATGGGAGGACTCGAGGGATTAATCAACATACTTGATTCCGAAGGAGGATTCGGTGCCCTTGAGAACCAGCTTCTCCCGGAGCAAGCAGCGTATTATATGAATTATGCTTCCAGAGTATCAAATCTTTCAGCTAAATCTCCAAAGGGCCCATCTGGTTTCCCTTTTCCTGGTCAGGAGAGTCATCCCGAAAATAATACTTCTATCAAAATGTTAGAAGCTGCAGTGCAGAGACTGACCAATTTATGCTCAGTTTTGAATGATATGGAGCCTATATGTGTACTAAACCATGTTTTTGTGTTAAGGGAGTACATGAGAGAATGCATCCTTGGGAACCTTAGGAGGAGATTGCTTTCAGCACTGAAAACTGATAATGATCTCCAACGGCCTTCTGTTCTGGAGTCACTTATTCGCAGACACATAAGCATCATCCATCTGGCAGAGCAGCATATTAGCATGGACCTAACTCAGGGTATTCGAGAAGTTCTGCTCTCAGAGGCCTTCTCAGGACCAGTTTCTTCTTTGCATTTGTTTGAGAAGCCAGCAGAGCAACACACAGGATCAGCCACTGAGGCTGTTTGTAACTGGTACATTGAAAATATAATCAAGGATATATCTGGAGCTGGAATCCTTTTTGCTCCAATTCACAAATGTTTCAAGAGCACAAGGCCTGTTGGTGGATATTTTGCTGATTCAGTCACCGATCTTAAAGAATTGAAGGCCTTTGTTCGGATTTTTGGTGGCTATGGAGTTGACAGGTTAGACAGAATGCTGAAAGAACACACTGCTGCACTTTTAAATTGTATTGACACATCATTGAGATCGAATCGTGACTTGCTAGAGGCAGTTGCTGGAAGCCTGCATTCTGGTGACCGAATAGAAAGAGATGCATCAATAAAGCAGATACTCGATATAGACACTGTGATTGGGTTTTGCGTCCAAGCTGGGCTGGCCTTGGCTTTTGACAGACTTCTAGCTGAAGCTTCTGGAGCTGTGCTCTTAGAAGGTGCACCCTTGATACATTCGTTGTTGACCGGGATAGCTAAGCATATACCTGAAGAAATACCTGAAAGGATAGAAATCAGGAGATTGAGAGGGGTGGCAAACAATTTTGGTGTAGTCTACGATCATGATTCTCAGTGGGTGAGATTTATCTTAGAAGAGGTTGGAGGTGCAAACGATGGTTCATGGAGCTTCTTGCCTTATTTATTTGCCACTTTCATGATGTCAAATATTTGGAATACAACTGCCTTCAACGTGGACACAGGGGGATTCAACAACAATATCCACTGCTTGGCAAGGTGCATTAGTGCTGTAATTGCGGGAAGTGAGTTTGTTAGACTGGAGTGTGAGCATCAACAAAGACAGTCTCTCTCAAATGGACATGCTGCTGATAATGGGGACACTGAAATGCAAAGCCGTTCGTCAGCTGAAGCAAGCATAAAATCCACAATGCAGCTCTTCGTCAAATTCTCCGCAGGCATTATACTGGATTCATGGAATGAAACTAACCGATCTCATCTCGTAGCACAGCTAATTTTCCTAGACCAACTTTGTGAGATCTCGCCTTATCTTCCACGAAGCTCTCTGGAGCCCCATGTTCCATACGCCATTCTTCGTTCGATCTACAGCCAGTATTACGAAAACTCTCCTTCGACGCCACTTGCATTACTAAGTGGTTCACCCCGCCATTCACCTGCTGCATCACTCTCACATTCATCTCCTGGAGTACGACATCCTCGCGGCGACTCCACTCCTCAGTATGATTCAAGTTATTTCAAAGGATCATCATCCCATAGCCAGGAGCATGAAACTGACAGCGGAAGCTTACGGAGCACAGAAAGCAGGCTAAGGAATGTTCGTCGTTCCGGGCCTTTAGATTACAGCTCAAGCCGTAGTAAAGTTAAGTTTGTGGAAGGCTCAACTTCGGGGAGTACCGGCCCCAGTCCGCTACCGAGGTTCGCTGTCTCAAGATCCGGTCCAATATCCTACAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006355 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008150 GO:0009653 GO:0009825 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010090 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010638 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0030029 GO:0030036 GO:0030154 GO:0030832 GO:0030833 GO:0030838 GO:0031209 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031984 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0032991 GO:0033043 GO:0040007 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045010 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051493 GO:0051495 GO:0060255 GO:0065007 GO:0065008 GO:0071840 GO:0080090 GO:0090066 GO:0090558 GO:0090626 GO:0097435 GO:0098827 GO:0110053 GO:1902680 GO:1902903 GO:1902905 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04810 map04810

Pfam Domains

Protein Families

Nckap1

Protein Analysis

1382

Amino Acids

154.77

Weight (kDa)

6.25

Isoelectric Point (pI)

52.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nckap1	PF09735	75 - 1264	0.0	Nck-associated protein 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0008534)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35110 AT2G35110
fragaria_vesca	FvH4_3g12410 FvH4_3g12410
malus_domestica	MD05G1250500.v1.1 MD10G1231300.v1.1
prunus_persica	Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1
pyrus_communis	pycom05g22840 pycom10g19450
rosa_chinensis	RchiOBHm_Chr5g0020301
rosa_laevigata	RLG00000032516
rosa_multiflora	Rmu_sc0002087.1_g000004 Rmu_sc0007157.1_g000007
rosa_roxburghii	Rroxscaffold_1G00057910
rosa_rugosa	Rorug05G0058400
rosa_samantha	Rh5AG148800 Rh5BG148400 Rh5CG159900 Rh5DG147200
rosa_wichuraiana	Rw5G013180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3853
Acc36I	ACCTGC	1 cut(s)	3853
Acc65I	GGTACC	1 cut(s)	1468
AccB1I	GGYRCC	2 cut(s)	1468, 2204
AccB7I	CCANNNNNTGG	3 cut(s)	277, 809, 3442
AccI	GTMKAC	1 cut(s)	3270
AccII	CGCG	3 cut(s)	630, 1026, 3891
AciI	CCGC	8 cut(s)	183, 1715, 3470, 3624, 3835, 3891, 3969, 4097
AcoI	YGGCCR	1 cut(s)	298
AcsI	RAATTY	8 cut(s)	130, 148, 273, 790, 925, 1140, 1757, 3617
AcuI	CTGAAG	5 cut(s)	249, 1972, 3147, 3225, 3600
AcyI	GRCGYC	1 cut(s)	3806
AflII	CTTAAG	1 cut(s)	1946
AflIII	ACRYGT	3 cut(s)	628, 1426, 1742
AjnI	CCWGG	5 cut(s)	143, 1630, 2317, 3871, 3948
AjuI	GAANNNNNNNTTGG	4 cut(s)	260, 292, 2460, 2492
Alw21I	GWGCWC	6 cut(s)	143, 949, 2813, 3147, 3160, 3986
Alw26I	GTCTC	7 cut(s)	7, 107, 373, 699, 2374, 3522, 4120
Alw44I	GTGCAC	1 cut(s)	3156
AlwNI	CAGNNNCTG	3 cut(s)	2383, 2717, 2999
Ama87I	CYCGRG	1 cut(s)	2162
ApaI	GGGCCC	1 cut(s)	2300
ApaLI	GTGCAC	1 cut(s)	3156
ApoI	RAATTY	8 cut(s)	130, 148, 273, 790, 925, 1140, 1757, 3617
ArsI	GACNNNNNNTTYG	2 cut(s)	776, 808
AseI	ATTAAT	1 cut(s)	2171
Asp718I	GGTACC	1 cut(s)	1468
AsuC2I	CCSGG	5 cut(s)	287, 1804, 2228, 3184, 4018
AsuHPI	GGTGA	8 cut(s)	39, 958, 1733, 2839, 3029, 3304, 3822, 3834
AsuII	TTCGAA	1 cut(s)	1762
AvaI	CYCGRG	1 cut(s)	2162
AxyI	CCTNAGG	1 cut(s)	2486
BaeGI	GKGCMC	3 cut(s)	2209, 2300, 3160
BaeI	ACNNNNGTAYC	2 cut(s)	3158, 3191
BalI	TGGCCA	1 cut(s)	300
BamHI	GGATCC	2 cut(s)	289, 483
BanI	GGYRCC	2 cut(s)	1468, 2204
BanII	GRGCYC	5 cut(s)	143, 803, 949, 2300, 3744
BauI	CACGAG	1 cut(s)	66
BbsI	GAAGAC	2 cut(s)	789, 1978
Bbv12I	GWGCWC	6 cut(s)	143, 949, 2813, 3147, 3160, 3986
BceAI	ACGGC	3 cut(s)	2549, 3552, 4026
BciT130I	CCWGG	5 cut(s)	145, 1632, 2319, 3873, 3950
BclI	TGATCA	1 cut(s)	1339
BcnI	CCSGG	5 cut(s)	287, 1804, 2228, 3184, 4018
BcoDI	GTCTC	7 cut(s)	7, 107, 373, 699, 2374, 3522, 4120
BfaI	CTAG	8 cut(s)	27, 821, 956, 1032, 1347, 2987, 3122, 3692
BfmI	CTRYAG	3 cut(s)	1839, 2370, 3775
BfrI	CTTAAG	1 cut(s)	1946
BfuAI	ACCTGC	1 cut(s)	3853
BglI	GCCNNNNNGGC	1 cut(s)	2720
BglII	AGATCT	2 cut(s)	1195, 3708
BlpI	GCTNAGC	1 cut(s)	3192
BmcAI	AGTACT	1 cut(s)	101
BmeT110I	CYCGRG	1 cut(s)	2162
BmrI	ACTGGG	2 cut(s)	1860, 4086
BmuI	ACTGGG	2 cut(s)	1860, 4086
BpiI	GAAGAC	2 cut(s)	789, 1978
BplI	GAGNNNNNCTC	4 cut(s)	2636, 2668, 3129, 3161
BpmI	CTGGAG	8 cut(s)	127, 1220, 2567, 2787, 3156, 3512, 3758, 3894
Bpu10I	CCTNAGC	2 cut(s)	948, 3998
Bpu1102I	GCTNAGC	1 cut(s)	3192
Bpu14I	TTCGAA	1 cut(s)	1762
BpuEI	CTTGAG	3 cut(s)	2231, 4021, 4102
BpuMI	CCSGG	5 cut(s)	287, 1804, 2228, 3184, 4018
BsaAI	YACGTR	1 cut(s)	806
BsaBI	GATNNNNATC	4 cut(s)	723, 2523, 3227, 3937
BsaHI	GRCGYC	1 cut(s)	3806
BsaI	GGTCTC	1 cut(s)	699
BsaJI	CCNNGG	8 cut(s)	479, 656, 1386, 1630, 1631, 2476, 3102, 4103
BsaWI	WCCGGW	2 cut(s)	1611, 4124
BsaXI	ACNNNNNCTCC	2 cut(s)	106, 136
Bse118I	RCCGGY	1 cut(s)	4085
Bse1I	ACTGG	9 cut(s)	293, 1855, 1920, 2660, 2738, 3495, 3642, 3781, 4092
Bse21I	CCTNAGG	1 cut(s)	2486
Bse3DI	GCAATG	2 cut(s)	1554, 2026
Bse8I	GATNNNNATC	4 cut(s)	723, 2523, 3227, 3937
BseBI	CCWGG	5 cut(s)	145, 1632, 2319, 3873, 3950
BseDI	CCNNGG	8 cut(s)	479, 656, 1386, 1630, 1631, 2476, 3102, 4103
BseJI	GATNNNNATC	4 cut(s)	723, 2523, 3227, 3937
BseMI	GCAATG	2 cut(s)	1554, 2026
BseNI	ACTGG	9 cut(s)	293, 1855, 1920, 2660, 2738, 3495, 3642, 3781, 4092
BseRI	GAGGAG	4 cut(s)	151, 1303, 2506, 3894
BseSI	GKGCMC	3 cut(s)	2209, 2300, 3160
BseYI	CCCAGC	1 cut(s)	3092
BsgI	GTGCAG	2 cut(s)	2396, 2922
Bsh1236I	CGCG	3 cut(s)	630, 1026, 3891
BshNI	GGYRCC	2 cut(s)	1468, 2204
BsiHKAI	GWGCWC	6 cut(s)	143, 949, 2813, 3147, 3160, 3986
BsiHKCI	CYCGRG	1 cut(s)	2162
BsiSI	CCGG	8 cut(s)	287, 1612, 1804, 2228, 3183, 4017, 4086, 4125
BsiWI	CGTACG	1 cut(s)	1175
BslFI	GGGAC	2 cut(s)	846, 3563
BsmAI	GTCTC	7 cut(s)	7, 107, 373, 699, 2374, 3522, 4120
BsmFI	GGGAC	2 cut(s)	846, 3563
BsmI	GAATGC	4 cut(s)	691, 2474, 2922, 3010
Bso31I	GGTCTC	1 cut(s)	699
BsoBI	CYCGRG	1 cut(s)	2162
Bsp119I	TTCGAA	1 cut(s)	1762
Bsp120I	GGGCCC	1 cut(s)	2296
Bsp1720I	GCTNAGC	1 cut(s)	3192
Bsp19I	CCATGG	1 cut(s)	479
BspACI	CCGC	8 cut(s)	183, 1715, 3470, 3624, 3835, 3891, 3969, 4097
BspFNI	CGCG	3 cut(s)	630, 1026, 3891
BspHI	TCATGA	2 cut(s)	3277, 3369
BspMAI	CTGCAG	1 cut(s)	2374
BspMI	ACCTGC	1 cut(s)	3853
BspQI	GCTCTTC	1 cut(s)	3614
BspT104I	TTCGAA	1 cut(s)	1762
BspT107I	GGYRCC	2 cut(s)	1468, 2204
BspTI	CTTAAG	1 cut(s)	1946
BspTNI	GGTCTC	1 cut(s)	699
BsrDI	GCAATG	2 cut(s)	1554, 2026
BsrFI	RCCGGY	1 cut(s)	4085
BsrI	ACTGG	9 cut(s)	293, 1855, 1920, 2660, 2738, 3495, 3642, 3781, 4092
BssAI	RCCGGY	1 cut(s)	4085
BssECI	CCNNGG	8 cut(s)	479, 656, 1386, 1630, 1631, 2476, 3102, 4103
BssNI	GRCGYC	1 cut(s)	3806
BssSI	CACGAG	1 cut(s)	66
BssT1I	CCWWGG	5 cut(s)	479, 656, 1386, 2476, 3102
Bst2BI	CACGAG	1 cut(s)	66
Bst2UI	CCWGG	5 cut(s)	145, 1632, 2319, 3873, 3950
Bst4CI	ACNGT	8 cut(s)	45, 556, 752, 765, 1843, 1956, 3070, 3516
Bst6I	CTCTTC	3 cut(s)	2085, 3303, 3614
BstACI	GRCGYC	1 cut(s)	3806
BstAFI	CTTAAG	1 cut(s)	1946
BstBAI	YACGTR	1 cut(s)	806
BstBI	TTCGAA	1 cut(s)	1762
BstC8I	GCNNGC	9 cut(s)	1254, 1938, 2235, 2691, 3008, 3099, 3586, 3628, 3995
BstDSI	CCRYGG	1 cut(s)	479
BstEII	GGTNACC	1 cut(s)	3017
BstENI	CCTNNNNNAGG	1 cut(s)	2615
BstFNI	CGCG	3 cut(s)	630, 1026, 3891
BstMAI	GTCTC	7 cut(s)	7, 107, 373, 699, 2374, 3522, 4120
BstMWI	GCNNNNNNNGC	8 cut(s)	1032, 2695, 2720, 3098, 3137, 3467, 3981, 3990
BstNI	CCWGG	5 cut(s)	145, 1632, 2319, 3873, 3950
BstNSI	RCATGY	6 cut(s)	208, 599, 1430, 1746, 1940, 3536
BstPI	GGTNACC	1 cut(s)	3017
BstSFI	CTRYAG	3 cut(s)	1839, 2370, 3775
BstSLI	GKGCMC	3 cut(s)	2209, 2300, 3160
BstUI	CGCG	3 cut(s)	630, 1026, 3891
BstV2I	GAAGAC	2 cut(s)	789, 1978
BstX2I	RGATCY	8 cut(s)	289, 483, 541, 607, 952, 1195, 3708, 4121
BstXI	CCANNNNNNTGG	3 cut(s)	90, 1430, 1465
BstYI	RGATCY	8 cut(s)	289, 483, 541, 607, 952, 1195, 3708, 4121
Bsu36I	CCTNAGG	1 cut(s)	2486
BtgI	CCRYGG	1 cut(s)	479
BtsI	GCAGTG	4 cut(s)	215, 2379, 2931, 3436
BveI	ACCTGC	1 cut(s)	3853
Cac8I	GCNNGC	9 cut(s)	1254, 1938, 2235, 2691, 3008, 3099, 3586, 3628, 3995
CaiI	CAGNNNCTG	3 cut(s)	2383, 2717, 2999
CciI	TCATGA	2 cut(s)	3277, 3369
Cfr10I	RCCGGY	1 cut(s)	4085
CseI	GACGC	3 cut(s)	1015, 3073, 3814
DraI	TTTAAA	1 cut(s)	2946
EaeI	YGGCCR	1 cut(s)	298
Eam1104I	CTCTTC	3 cut(s)	2085, 3303, 3614
EarI	CTCTTC	3 cut(s)	2085, 3303, 3614
Ecl136II	GAGCTC	2 cut(s)	141, 947
Eco130I	CCWWGG	5 cut(s)	479, 656, 1386, 2476, 3102
Eco147I	AGGCCT	4 cut(s)	243, 2648, 2818, 2870
Eco24I	GRGCYC	5 cut(s)	143, 803, 949, 2300, 3744
Eco31I	GGTCTC	1 cut(s)	699
Eco32I	GATATC	1 cut(s)	908
Eco53kI	GAGCTC	2 cut(s)	141, 947
Eco57I	CTGAAG	5 cut(s)	249, 1972, 3147, 3225, 3600
Eco81I	CCTNAGG	1 cut(s)	2486
Eco88I	CYCGRG	1 cut(s)	2162
Eco91I	GGTNACC	1 cut(s)	3017
EcoICRI	GAGCTC	2 cut(s)	141, 947
EcoNI	CCTNNNNNAGG	1 cut(s)	2615
EcoO109I	RGGNCCY	2 cut(s)	833, 2296
EcoO65I	GGTNACC	1 cut(s)	3017
EcoRI	GAATTC	1 cut(s)	148
EcoRII	CCWGG	5 cut(s)	143, 1630, 2317, 3871, 3948
EcoRV	GATATC	1 cut(s)	908
EcoT14I	CCWWGG	5 cut(s)	479, 656, 1386, 2476, 3102
EcoT22I	ATGCAT	4 cut(s)	601, 1579, 2474, 3040
EcoT38I	GRGCYC	5 cut(s)	143, 803, 949, 2300, 3744
ErhI	CCWWGG	5 cut(s)	479, 656, 1386, 2476, 3102
FaqI	GGGAC	2 cut(s)	846, 3563
FauI	CCCGC	2 cut(s)	3463, 3842
FbaI	TGATCA	1 cut(s)	1339
FblI	GTMKAC	1 cut(s)	3270
FriOI	GRGCYC	5 cut(s)	143, 803, 949, 2300, 3744
FspBI	CTAG	8 cut(s)	27, 821, 956, 1032, 1347, 2987, 3122, 3692
GsaI	CCCAGC	1 cut(s)	3096
GsuI	CTGGAG	8 cut(s)	127, 1220, 2567, 2787, 3156, 3512, 3758, 3894
HapII	CCGG	8 cut(s)	287, 1612, 1804, 2228, 3183, 4017, 4086, 4125
HgaI	GACGC	3 cut(s)	1015, 3073, 3814
Hin1I	GRCGYC	1 cut(s)	3806
HincII	GTYRAC	6 cut(s)	634, 852, 1477, 1909, 2902, 3180
HindII	GTYRAC	6 cut(s)	634, 852, 1477, 1909, 2902, 3180
HindIII	AAGCTT	4 cut(s)	469, 1660, 3129, 3973
HpaI	GTTAAC	1 cut(s)	634
HpaII	CCGG	8 cut(s)	287, 1612, 1804, 2228, 3183, 4017, 4086, 4125
HphI	GGTGA	8 cut(s)	39, 958, 1733, 2839, 3029, 3304, 3822, 3834
Hpy99I	CGWCG	2 cut(s)	3808, 4014
HpyCH4III	ACNGT	8 cut(s)	45, 556, 752, 765, 1843, 1956, 3070, 3516
HpyCH4IV	ACGT	3 cut(s)	412, 805, 3405
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1032, 2695, 2720, 3098, 3137, 3467, 3981, 3990
HpySE526I	ACGT	3 cut(s)	412, 805, 3405
Hsp92I	GRCGYC	1 cut(s)	3806
KpnI	GGTACC	1 cut(s)	1472
Ksp22I	TGATCA	1 cut(s)	1339
KspAI	GTTAAC	1 cut(s)	634
LguI	GCTCTTC	1 cut(s)	3614
MaeI	CTAG	8 cut(s)	27, 821, 956, 1032, 1347, 2987, 3122, 3692
MaeII	ACGT	3 cut(s)	412, 805, 3405
MfeI	CAATTG	2 cut(s)	1891, 2079
MflI	RGATCY	8 cut(s)	289, 483, 541, 607, 952, 1195, 3708, 4121
MlsI	TGGCCA	1 cut(s)	300
MluI	ACGCGT	1 cut(s)	628
MluNI	TGGCCA	1 cut(s)	300
MlyI	GAGTC	5 cut(s)	1951, 2154, 2338, 2558, 3890
MmeI	TCCRAC	4 cut(s)	1447, 1520, 2554, 3295
Mox20I	TGGCCA	1 cut(s)	300
Mph1103I	ATGCAT	4 cut(s)	601, 1579, 2474, 3040
MscI	TGGCCA	1 cut(s)	300
MseI	TTAA	9 cut(s)	633, 776, 887, 1947, 2171, 2450, 2856, 2945, 4053
MslI	CAYNNNNRTG	8 cut(s)	70, 88, 1370, 1463, 2031, 3015, 3368, 3557
Msp20I	TGGCCA	1 cut(s)	300
MspA1I	CMGCKG	3 cut(s)	490, 3578, 3969
MspCI	CTTAAG	1 cut(s)	1946
MspI	CCGG	8 cut(s)	287, 1612, 1804, 2228, 3183, 4017, 4086, 4125
MunI	CAATTG	2 cut(s)	1891, 2079
Mva1269I	GAATGC	4 cut(s)	691, 2474, 2922, 3010
MvaI	CCWGG	5 cut(s)	145, 1632, 2319, 3873, 3950
MvnI	CGCG	3 cut(s)	630, 1026, 3891
MwoI	GCNNNNNNNGC	8 cut(s)	1032, 2695, 2720, 3098, 3137, 3467, 3981, 3990
NciI	CCSGG	5 cut(s)	287, 1804, 2228, 3184, 4018
NcoI	CCATGG	1 cut(s)	479
NmuCI	GTSAC	8 cut(s)	45, 964, 1027, 1792, 2550, 2845, 2978, 3017
NsiI	ATGCAT	4 cut(s)	601, 1579, 2474, 3040
NspI	RCATGY	6 cut(s)	208, 599, 1430, 1746, 1940, 3536
NspV	TTCGAA	1 cut(s)	1762
PaeI	GCATGC	1 cut(s)	1940
PaeR7I	CTCGAG	1 cut(s)	2162
PagI	TCATGA	2 cut(s)	3277, 3369
PaqCI	CACCTGC	1 cut(s)	3853
PasI	CCCWGGG	1 cut(s)	1631
PceI	AGGCCT	4 cut(s)	243, 2648, 2818, 2870
PciI	ACATGT	2 cut(s)	1426, 1742
PciSI	GCTCTTC	1 cut(s)	3614
PctI	GAATGC	4 cut(s)	691, 2474, 2922, 3010
Pfl23II	CGTACG	1 cut(s)	1175
PflMI	CCANNNNNTGG	3 cut(s)	277, 809, 3442
PfoI	TCCNGGA	3 cut(s)	143, 2226, 3871
PleI	GAGTC	5 cut(s)	1950, 2154, 2337, 2557, 3890
PpsI	GAGTC	5 cut(s)	1950, 2154, 2337, 2557, 3890
Ppu21I	YACGTR	1 cut(s)	806
PpuMI	RGGWCCY	1 cut(s)	833
PscI	ACATGT	2 cut(s)	1426, 1742
PshBI	ATTAAT	1 cut(s)	2171
Psp124BI	GAGCTC	2 cut(s)	143, 949
Psp5II	RGGWCCY	1 cut(s)	833
Psp6I	CCWGG	5 cut(s)	143, 1630, 2317, 3871, 3948
PspEI	GGTNACC	1 cut(s)	3017
PspFI	CCCAGC	1 cut(s)	3092
PspGI	CCWGG	5 cut(s)	143, 1630, 2317, 3871, 3948
PspLI	CGTACG	1 cut(s)	1175
PspOMI	GGGCCC	1 cut(s)	2296
PspPPI	RGGWCCY	1 cut(s)	833
PspXI	VCTCGAGB	1 cut(s)	2162
PsrI	GAACNNNNNNTAC	4 cut(s)	447, 479, 510, 542
PstI	CTGCAG	1 cut(s)	2374
PstNI	CAGNNNCTG	3 cut(s)	2383, 2717, 2999
PsuI	RGATCY	8 cut(s)	289, 483, 541, 607, 952, 1195, 3708, 4121
PvuII	CAGCTG	2 cut(s)	490, 3578
RseI	CAYNNNNRTG	8 cut(s)	70, 88, 1370, 1463, 2031, 3015, 3368, 3557
SacI	GAGCTC	2 cut(s)	143, 949
SapI	GCTCTTC	1 cut(s)	3614
SaqAI	TTAA	9 cut(s)	633, 776, 887, 1947, 2171, 2450, 2856, 2945, 4053
ScaI	AGTACT	1 cut(s)	101
SchI	GAGTC	5 cut(s)	1951, 2154, 2338, 2558, 3890
SfcI	CTRYAG	3 cut(s)	1839, 2370, 3775
Sfr274I	CTCGAG	1 cut(s)	2162
SfuI	TTCGAA	1 cut(s)	1762
SlaI	CTCGAG	1 cut(s)	2162
SmiMI	CAYNNNNRTG	8 cut(s)	70, 88, 1370, 1463, 2031, 3015, 3368, 3557
SmlI	CTYRAG	5 cut(s)	1946, 2162, 2210, 4036, 4117
SmoI	CTYRAG	5 cut(s)	1946, 2162, 2210, 4036, 4117
SphI	GCATGC	1 cut(s)	1940
SseBI	AGGCCT	4 cut(s)	243, 2648, 2818, 2870
SsiI	CCGC	8 cut(s)	183, 1715, 3470, 3624, 3835, 3891, 3969, 4097
SspI	AATATT	2 cut(s)	1782, 3382
SspMI	CTAG	8 cut(s)	27, 821, 956, 1032, 1347, 2987, 3122, 3692
SstI	GAGCTC	2 cut(s)	143, 949
StuI	AGGCCT	4 cut(s)	243, 2648, 2818, 2870
StyI	CCWWGG	5 cut(s)	479, 656, 1386, 2476, 3102
TaaI	ACNGT	8 cut(s)	45, 556, 752, 765, 1843, 1956, 3070, 3516
TaiI	ACGT	3 cut(s)	415, 808, 3408
TaqII	GACCGA	2 cut(s)	723, 3036
TatI	WGTACW	5 cut(s)	99, 551, 1551, 2428, 2457
TauI	GCSGC	2 cut(s)	186, 3894
Tru1I	TTAA	9 cut(s)	633, 776, 887, 1947, 2171, 2450, 2856, 2945, 4053
Tru9I	TTAA	9 cut(s)	633, 776, 887, 1947, 2171, 2450, 2856, 2945, 4053
TseFI	GTSAC	8 cut(s)	45, 964, 1027, 1792, 2550, 2845, 2978, 3017
Tsp45I	GTSAC	8 cut(s)	45, 964, 1027, 1792, 2550, 2845, 2978, 3017
TspGWI	ACGGA	1 cut(s)	3994
Van91I	CCANNNNNTGG	3 cut(s)	277, 809, 3442
Vha464I	CTTAAG	1 cut(s)	1946
VneI	GTGCAC	1 cut(s)	3156
VspI	ATTAAT	1 cut(s)	2171
XagI	CCTNNNNNAGG	1 cut(s)	2615
XapI	RAATTY	8 cut(s)	130, 148, 273, 790, 925, 1140, 1757, 3617
XceI	RCATGY	6 cut(s)	208, 599, 1430, 1746, 1940, 3536
XhoI	CTCGAG	1 cut(s)	2162
XmiI	GTMKAC	1 cut(s)	3270
XspI	CTAG	8 cut(s)	27, 821, 956, 1032, 1347, 2987, 3122, 3692
ZrmI	AGTACT	1 cut(s)	101
Zsp2I	ATGCAT	4 cut(s)	601, 1579, 2474, 3040

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.