Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0008534

Physical Location & Seq

Genomic Coordinates Forward (+)

7381051 .. 7393039

Gene Structure 11989 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g12410.t6

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4143 bp

ATGGCGAAATCGAGACAACATTTCTCTAGCCAAGATTCGTCATTATCACCCACTTCTGTGAGATCAAAAGAGTGGGAGGGTCCGTCGAGATGGACTGAGTACTTGGGTCCAGAACCTTCTCCCATGACATCTAGGAGCTCCAGGACTGCAGGTCCTGATGGACAGATTGTCCAAAGTGCAGGCGTGTCCCACAAAGGCTTAAACATGCAGTGGGTAGTTCAACTAACTGAAGTAGCAGAAGGTCTTATGGCTAAAATGTATAGGCTAAACCAAATTTTGGATTACCCGGATCCAGTTGGACATACATTTTCAGAAGCCTTTTGGAAAGCCGGTGTGTTCCCCAACCATCCAAGACTTTGCTTGCTACTTTCAAAGAAGTTCCCGGAGCATTATAGCAAGTTACAGCTTGAACGTGTTGATAAGGTTGCCTTGGACGCCTTGCATGATAATGCAGGGCTTCATCTACAAAGCTTGGAACCATGGGTTCAGCTGCTTCTTGACTTGATGGCATTTCGTGAACAAGCTCTACGGCTTATATTGGATCTAAGCAGTACTGTTATTACCTTGTTGCCTCACCAGAACTCGCTTATACTACATGCATTTATGGATCTCTTCTGTTCATTTGTCCGTGTTAACCTATTCTCTGAGAAGATACCGAGGAAAATGATGCTACAAATGTATAACCTTTTGCATGCAATATCAAGAAATGACCGAGATTGTGATTTCTATCATCGGTTGGTTCAATTTATAGACGGTTATGATCCACCCTTGAAAGGATTGAAAGAAGACCTAAATTTTGTGAGCCCCCGTATTGGAGAGGTGCTAGAGGCTGTGGGTCCTATCATTTTTCTGTCAACAGATACAAGAAAGCTCCGAAACGAGGGATTCTTAAGTCCATATCATCCTCGATATCCAGACATCCTTACAAATTCTGCACACCCAATGAGAGCTCAGGATTTAGCGAATGTCACCTCGTATCGGGAATGGGTATTATTTGGGTACCTCGTTTGTCCAGATGAGCTTCTACGCGTCACTAGCATTGATATTGCTCTGGTCGTCTTGAAAGAAAATTTGGTTCTTACATTGTTCAGGGATGAGTACATACTTTTGCATGAAGAGTATCAGCTATTTGTCCTGCCACGAATTATAGAATCAAAGAAGATGGCAAAATCTGGACGTACAAAGCAAAAAGAAGCAGATTTGGAGTATAGTGTAGCAAAGCAAGTTGAGAAAATGATAAGTGAAGTGCATGAGCAAGCATTACTATCATGTGACGCCATCCACCGTGAGAGGAGGATATTACTGAAGCAAGAGATCGGAAGAATGGTACTTTTTTTCACTGATCAGCCTAGTTTGTTGGCTCCAAACATACAGATGGTATTCTCTGCCCTGGCTTTTGCTCAATGTGAGGTCCTCTGGTATTTCCAACATGTTGGAATTGCATCATCAAAATCAAAAGCCGTTCGAATGGTGTCAGTTGAAATTGATCCAAGTGATCCAACGATTGGGTTTTTGTTAGATGGAATGGACCATCTTTGCTGTTTGGTACGCAAGTATATTGCAGCAATTAGAGGTTATGCATTGTCGTACCTTTCTTCTTGTGCTGGTAGAATCCGGTTTTTGTTAAATACCCCTGGGATGGTGGCTCTCGACTTAGATGCAAGCTTGAAAAGTCTTTTTCAGCAGATTGTTCAGCACCTTGAAAACATTCCAAAACCACAGGGTGAAAATGTATCTGCCATTACATGTGATCTATCTGAATTTCGAAAGGATTGGTTATCAATATTGATGATAGTGACATCATCCCGGTCATCTATAAACATAAGACATTTGGAGAAAGCTACAGTCTCTACAGGGAAAGAAGGCCTGCTATCAGAGGGAAATGCTGCCTACAATTGGTCCAGATGTGTCGATGAACTGGAATCTCAATTATCAAAGCATGCGAGTCTTAAGAAGTTGTATTTCTACCATCAGCACCTTACAGCGGTCTTCAGGAACACTATGTTTGGCCCGGAAGGTCGCCCACAACATTGCTGTGCCTGGCTTGGTGTTGCAAGTAGTTTTCCAGAGTGTGCTTCCCCCATTGTTCCGGAAGAGGTAACCAAGATTGGCAGAGATGCAGTCCTTTATGTTGAATCTCTCATTGAATCTATAATGGGAGGACTGGAGGGATTAATTAACATCCTTGATTCTGAAGGAGGATTTGGTGCCCTAGAGAACCAGCTTCTTCCGGAGCAGGCCGCTTCTTATATGAATTCTGCTTCAAGAATATCAATAACTTCAACTAAATCTCCAAAAGGAGCACCGGGTTTCCCTTTGCCTGGCCATGAGAGTCGTCCTGAAAATAATGCTTCTATCAAGATGTTAGAAGCTGCGGTGCAGAGATTGACCAACTTATGCTCAGTTTTGAATGATATGGAGCCTATATGTGTTCTAAACCATGTTTTTGTCTTAAGGGAGTACATGAGGGAGTGCATTCTTGGGAACCTTAGGAGGAGATTGCTTGCAGTACTGAAAACTGACAATGACCTCCAACGGCCTACGGTTCTTGAGTTGCTTATTCGCAGACACATCAGCATTATCCATCTGGCAGAGCAGCATATTAGCATGGACTTAACTCAGGGTATTCGAGAGGTTTTGCTCTCAGAAGCCTTCTCAGGACCAGTTTCTTCTTTGCATTTGTTTGAAAAACCAGAAGAGCAACATACAGGATCAGCCACTGAAGCTGTCTGCAACTGGTACATTGAAAATATAATCAAGGATATATCAGGTGCTGGAATCCTTTTTGCTCCAATTCACAAATGCTTCAAGAGCACAAGGCCTGTTGGTGGATATTTTGCTGATTCAGTCACTGATCTTAGAGAATTAAAGGCCTTTGCTCGCATCTTTGGTGGCTATGGAGTTGACAGGCTAGACAGATTACTGAAAGAACACACTGCTGCACTTTTAAATTGTATTGATACATCACTGAGATCAAATCGTGATGTGCTAGAGGCAGTTGCTGGTAGTCTGCATTCTGGAGACCGAAATGAAAGAGAGGCTTCAATAAAGCAGATAGTCGATATAGACACGGTGATTGGGTTTTGTGTTCAAGCTGGCTTGGCCCTGGCTTTTGACAGATCTCTAGCTGAAGCTGCTGGCACTGTACTTTTTGAAGGTGCACCCTTGATACATTCATTATTAGCTGGAATATCTAAGCATATACCTGAAGAAATACCTGAAAAGAAAGAAGTCAGGAGATTGAGAGGGGTGGCAAACAATGTTGGTGTAGTTGATAATCATGATTCCCAGTGGGTGAGATTGATCTTAGAAGAGGTTGGGGGTGCAAATGATGGGTCATGGAGCCTATTACCCTATTTCTTTGCCGCCTTTATGACATCTAATATATGGACTACCACAGCCTTCAATGTGGACACTGGGGGTTTCAATAACAATATTCATTGCTTGGCAAGGTGCATCAGTGCTGTAATTGCAGGAAGTGAGTTTGTTAGAGTGGAACGTGAATATCAGCAAAGACAATCTCTCTCAAATGGCCATGTTGGTGAATCAGTGGACTCTGAACAAAGTCGTTTGTCAGCTGAAGCAAGCATAAAATCCACAATGCAGCTTTTCGTCAAATTCTCTGCAAGCATTATACTGGATTCTTGGAGTGAAACTAATAGATCTCATCTTGTAGCACAGCTAATTTTCCTGGACCAACTCTGCGAGATCTCACCTTATCTTCCAAGAAGCTCTCTGGAACCCCATGTTCCATATGCCATTCTTCGTTCAATATACAGCCAGTACTATACAAACTCTCCAAATACACCACTCGCATTACTAAGCATTTCACCCCGCCATTCGCCTGCTGTATCGCTCTCACATGCATCTCCCGCAGCACGACAACCCCGTGGCGACTCCACTCCTCAGTATGATTCAGGTTATTTCAAAGGATCATCATCCCATGGCCAAGAGCACATTTATGATGGTGGAAGTTCACGAAGCACTGAACACAGGCAACAGAACTATCGCCGTTCTGGGCCTTTAGATTATGGCTCAAGCCGAAAAGTTAAGTTTGCGGAGGGATCAACTTCAGGGAACACAGGTCCCAGTCCACTACCAAGATTTGCTGTCTCAAGGTCTGGTCCAATATCATACAAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006355 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008150 GO:0009653 GO:0009825 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010090 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010638 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0030029 GO:0030036 GO:0030154 GO:0030832 GO:0030833 GO:0030838 GO:0031209 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031984 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0032991 GO:0033043 GO:0040007 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045010 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051493 GO:0051495 GO:0060255 GO:0065007 GO:0065008 GO:0071840 GO:0080090 GO:0090066 GO:0090558 GO:0090626 GO:0097435 GO:0098827 GO:0110053 GO:1902680 GO:1902903 GO:1902905 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04810 map04810

Pfam Domains

Protein Families

Nckap1

Protein Analysis

1381

Amino Acids

154.17

Weight (kDa)

6.47

Isoelectric Point (pI)

48.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nckap1	PF09735	5 - 597	2.2e-147	Nck-associated protein 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008534)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35110 AT2G35110
fragaria_vesca	FvH4_3g12410 FvH4_3g12410
malus_domestica	MD05G1250500.v1.1 MD10G1231300.v1.1
prunus_persica	Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1
pyrus_communis	pycom05g22840 pycom10g19450
rosa_chinensis	RchiOBHm_Chr5g0020301
rosa_laevigata	RLG00000032516
rosa_multiflora	Rmu_sc0002087.1_g000004 Rmu_sc0007157.1_g000007
rosa_roxburghii	Rroxscaffold_1G00057910
rosa_rugosa	Rorug05G0058400
rosa_samantha	Rh5AG148800 Rh5BG148400 Rh5CG159900 Rh5DG147200
rosa_wichuraiana	Rw5G013180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	140
Acc65I	GGTACC	1 cut(s)	999
AccB1I	GGYRCC	2 cut(s)	999, 2207
AccB7I	CCANNNNNTGG	2 cut(s)	277, 1505
AccII	CGCG	1 cut(s)	1029
AccIII	TCCGGA	2 cut(s)	2089, 2230
AciI	CCGC	7 cut(s)	1985, 2241, 2375, 3366, 3835, 3873, 4058
AcoI	YGGCCR	3 cut(s)	2323, 3532, 3946
AcsI	RAATTY	7 cut(s)	273, 793, 928, 1069, 1760, 2254, 3617
AcuI	CTGAAG	9 cut(s)	249, 1325, 1975, 2214, 2742, 3150, 3228, 3600, 4056
AcyI	GRCGYC	2 cut(s)	435, 1275
AdeI	CACNNNGTG	1 cut(s)	1724
AflII	CTTAAG	3 cut(s)	889, 1949, 2452
AflIII	ACRYGT	4 cut(s)	412, 1027, 1429, 1745
AhdI	GACNNNNNGTC	2 cut(s)	150, 167
AjnI	CCWGG	7 cut(s)	140, 1389, 1633, 2039, 2320, 3105, 3690
AjuI	GAANNNNNNNTTGG	4 cut(s)	1055, 1087, 2187, 2219
AleI	CACNNNNGTG	1 cut(s)	56
Alw21I	GWGCWC	6 cut(s)	140, 952, 2305, 2816, 3163, 3957
Alw26I	GTCTC	4 cut(s)	7, 1852, 3015, 4117
Alw44I	GTGCAC	1 cut(s)	3159
AlwNI	CAGNNNCTG	5 cut(s)	155, 2720, 2774, 2852, 3002
Aor13HI	TCCGGA	2 cut(s)	2089, 2230
ApaLI	GTGCAC	1 cut(s)	3159
ApeKI	GCWGC	9 cut(s)	490, 1562, 1886, 2372, 2596, 2939, 3134, 3604, 3875
ApoI	RAATTY	7 cut(s)	273, 793, 928, 1069, 1760, 2254, 3617
ArsI	GACNNNNNNTTYG	4 cut(s)	779, 811, 3320, 3352
AseI	ATTAAT	1 cut(s)	2174
Asp700I	GAANNNNTTC	1 cut(s)	1707
Asp718I	GGTACC	1 cut(s)	999
AsuC2I	CCSGG	5 cut(s)	287, 383, 1807, 2012, 2307
AsuHPI	GGTGA	9 cut(s)	39, 566, 961, 1736, 3085, 3307, 3554, 3705, 3822
AsuII	TTCGAA	2 cut(s)	1465, 1765
AxyI	CCTNAGG	1 cut(s)	2489
BaeGI	GKGCMC	2 cut(s)	2212, 3163
BaeI	ACNNNNGTAYC	2 cut(s)	1538, 1571
BalI	TGGCCA	3 cut(s)	2325, 3534, 3948
BamHI	GGATCC	1 cut(s)	289
BanI	GGYRCC	2 cut(s)	999, 2207
BanII	GRGCYC	3 cut(s)	140, 806, 952
BbsI	GAAGAC	2 cut(s)	792, 1981
Bbv12I	GWGCWC	6 cut(s)	140, 952, 2305, 2816, 3163, 3957
BbvI	GCAGC	9 cut(s)	477, 1574, 1873, 2359, 2608, 2926, 3121, 3616, 3887
BceAI	ACGGC	4 cut(s)	545, 1445, 2552, 3996
BcgI	CGANNNNNNTGC	2 cut(s)	1640, 1674
BciT130I	CCWGG	7 cut(s)	142, 1391, 1635, 2041, 2322, 3107, 3692
BclI	TGATCA	1 cut(s)	1342
BcnI	CCSGG	5 cut(s)	287, 383, 1807, 2012, 2307
BcoDI	GTCTC	4 cut(s)	7, 1852, 3015, 4117
BfaI	CTAG	9 cut(s)	27, 132, 824, 1035, 1350, 2213, 2912, 2990, 3125
BfmI	CTRYAG	3 cut(s)	147, 1842, 1851
BfrI	CTTAAG	3 cut(s)	889, 1949, 2452
BfuAI	ACCTGC	1 cut(s)	140
BglII	AGATCT	3 cut(s)	3119, 3662, 3708
BmcAI	AGTACT	4 cut(s)	101, 553, 2511, 3785
BmeRI	GACNNNNNGTC	2 cut(s)	150, 167
BmrI	ACTGGG	3 cut(s)	3283, 3426, 4083
BmsI	GCATC	7 cut(s)	657, 1451, 1648, 2107, 2892, 3465, 3875
BmuI	ACTGGG	3 cut(s)	3283, 3426, 4083
BpiI	GAAGAC	2 cut(s)	792, 1981
BpmI	CTGGAG	3 cut(s)	124, 2186, 3039
Bpu10I	CCTNAGC	1 cut(s)	951
Bpu14I	TTCGAA	2 cut(s)	1465, 1765
BpuEI	CTTGAG	3 cut(s)	2570, 4021, 4099
BpuMI	CCSGG	5 cut(s)	287, 383, 1807, 2012, 2307
BsaBI	GATNNNNATC	2 cut(s)	726, 3937
BsaHI	GRCGYC	2 cut(s)	435, 1275
BsaI	GGTCTC	1 cut(s)	3015
BsaJI	CCNNGG	9 cut(s)	429, 479, 656, 1389, 1633, 1634, 3105, 3889, 3943
BsaWI	WCCGGW	3 cut(s)	1614, 2089, 2230
BsaXI	ACNNNNNCTCC	6 cut(s)	68, 98, 2275, 2305, 3781, 3811
Bse118I	RCCGGY	1 cut(s)	329
Bse21I	CCTNAGG	1 cut(s)	2489
Bse3DI	GCAATG	2 cut(s)	2029, 3439
Bse8I	GATNNNNATC	2 cut(s)	726, 3937
BseAI	TCCGGA	2 cut(s)	2089, 2230
BseBI	CCWGG	7 cut(s)	142, 1391, 1635, 2041, 2322, 3107, 3692
BseDI	CCNNGG	9 cut(s)	429, 479, 656, 1389, 1633, 1634, 3105, 3889, 3943
BseGI	GGATG	9 cut(s)	346, 901, 918, 1099, 1278, 1644, 1802, 2181, 3938
BseJI	GATNNNNATC	2 cut(s)	726, 3937
BseMI	GCAATG	2 cut(s)	2029, 3439
BseMII	CTCAG	9 cut(s)	87, 636, 965, 2415, 2633, 2658, 2670, 2960, 3920
BseRI	GAGGAG	3 cut(s)	1306, 2509, 3894
BseSI	GKGCMC	2 cut(s)	2212, 3163
BseXI	GCAGC	9 cut(s)	477, 1574, 1873, 2359, 2608, 2926, 3121, 3616, 3887
BsgI	GTGCAG	4 cut(s)	198, 918, 2399, 2925
Bsh1236I	CGCG	1 cut(s)	1029
BshNI	GGYRCC	2 cut(s)	999, 2207
BsiHKAI	GWGCWC	6 cut(s)	140, 952, 2305, 2816, 3163, 3957
BsiSI	CCGG	9 cut(s)	287, 330, 383, 1615, 1807, 2012, 2090, 2231, 2306
BslFI	GGGAC	2 cut(s)	172, 4071
BsmAI	GTCTC	4 cut(s)	7, 1852, 3015, 4117
BsmFI	GGGAC	2 cut(s)	172, 4071
BsmI	GAATGC	2 cut(s)	2475, 3013
Bso31I	GGTCTC	1 cut(s)	3015
Bsp119I	TTCGAA	2 cut(s)	1465, 1765
Bsp1286I	GDGCHC	8 cut(s)	140, 806, 952, 2212, 2305, 2816, 3163, 3957
Bsp13I	TCCGGA	2 cut(s)	2089, 2230
Bsp19I	CCATGG	2 cut(s)	479, 3943
BspACI	CCGC	7 cut(s)	1985, 2241, 2375, 3366, 3835, 3873, 4058
BspCNI	CTCAG	9 cut(s)	88, 637, 964, 2414, 2632, 2657, 2669, 2961, 3919
BspEI	TCCGGA	2 cut(s)	2089, 2230
BspFNI	CGCG	1 cut(s)	1029
BspHI	TCATGA	1 cut(s)	3280
BspMAI	CTGCAG	1 cut(s)	151
BspMI	ACCTGC	1 cut(s)	140
BspQI	GCTCTTC	1 cut(s)	2691
BspT104I	TTCGAA	2 cut(s)	1465, 1765
BspT107I	GGYRCC	2 cut(s)	999, 2207
BspTI	CTTAAG	3 cut(s)	889, 1949, 2452
BspTNI	GGTCTC	1 cut(s)	3015
BsrDI	GCAATG	2 cut(s)	2029, 3439
BsrFI	RCCGGY	1 cut(s)	329
BssAI	RCCGGY	1 cut(s)	329
BssECI	CCNNGG	9 cut(s)	429, 479, 656, 1389, 1633, 1634, 3105, 3889, 3943
BssNI	GRCGYC	2 cut(s)	435, 1275
BssT1I	CCWWGG	3 cut(s)	429, 479, 3943
Bst2UI	CCWGG	7 cut(s)	142, 1391, 1635, 2041, 2322, 3107, 3692
Bst4CI	ACNGT	7 cut(s)	556, 755, 1286, 1846, 2545, 3073, 3145
Bst6I	CTCTTC	5 cut(s)	617, 1110, 2088, 2691, 3306
BstACI	GRCGYC	2 cut(s)	435, 1275
BstAFI	CTTAAG	3 cut(s)	889, 1949, 2452
BstBI	TTCGAA	2 cut(s)	1465, 1765
BstDSI	CCRYGG	3 cut(s)	479, 3889, 3943
BstEII	GGTNACC	1 cut(s)	2098
BstF5I	GGATG	9 cut(s)	346, 901, 918, 1099, 1278, 1644, 1802, 2181, 3938
BstFNI	CGCG	1 cut(s)	1029
BstMAI	GTCTC	4 cut(s)	7, 1852, 3015, 4117
BstMWI	GCNNNNNNNGC	8 cut(s)	434, 1035, 2723, 2811, 3101, 3134, 3470, 3872
BstNI	CCWGG	7 cut(s)	142, 1391, 1635, 2041, 2322, 3107, 3692
BstNSI	RCATGY	7 cut(s)	208, 599, 695, 1433, 1749, 1943, 3866
BstPI	GGTNACC	1 cut(s)	2098
BstSFI	CTRYAG	3 cut(s)	147, 1842, 1851
BstSLI	GKGCMC	2 cut(s)	2212, 3163
BstUI	CGCG	1 cut(s)	1029
BstV1I	GCAGC	9 cut(s)	477, 1574, 1873, 2359, 2608, 2926, 3121, 3616, 3887
BstV2I	GAAGAC	2 cut(s)	792, 1981
BstX2I	RGATCY	6 cut(s)	289, 541, 607, 3119, 3662, 3708
BstXI	CCANNNNNNTGG	1 cut(s)	1433
BstYI	RGATCY	6 cut(s)	289, 541, 607, 3119, 3662, 3708
Bsu36I	CCTNAGG	1 cut(s)	2489
BtgI	CCRYGG	3 cut(s)	479, 3889, 3943
BtsCI	GGATG	9 cut(s)	346, 901, 918, 1099, 1278, 1644, 1802, 2181, 3938
BtsI	GCAGTG	2 cut(s)	215, 2934
BveI	ACCTGC	1 cut(s)	140
CaiI	CAGNNNCTG	5 cut(s)	155, 2720, 2774, 2852, 3002
CciI	TCATGA	1 cut(s)	3280
Cfr10I	RCCGGY	1 cut(s)	329
CseI	GACGC	3 cut(s)	443, 1018, 1283
DraI	TTTAAA	1 cut(s)	2949
DraIII	CACNNNGTG	1 cut(s)	1724
DriI	GACNNNNNGTC	2 cut(s)	150, 167
EaeI	YGGCCR	3 cut(s)	2323, 3532, 3946
Eam1104I	CTCTTC	5 cut(s)	617, 1110, 2088, 2691, 3306
Eam1105I	GACNNNNNGTC	2 cut(s)	150, 167
EarI	CTCTTC	5 cut(s)	617, 1110, 2088, 2691, 3306
Ecl136II	GAGCTC	2 cut(s)	138, 950
Eco130I	CCWWGG	3 cut(s)	429, 479, 3943
Eco147I	AGGCCT	3 cut(s)	1866, 2821, 2873
Eco24I	GRGCYC	3 cut(s)	140, 806, 952
Eco31I	GGTCTC	1 cut(s)	3015
Eco32I	GATATC	1 cut(s)	911
Eco53kI	GAGCTC	2 cut(s)	138, 950
Eco57I	CTGAAG	9 cut(s)	249, 1325, 1975, 2214, 2742, 3150, 3228, 3600, 4056
Eco81I	CCTNAGG	1 cut(s)	2489
Eco91I	GGTNACC	1 cut(s)	2098
EcoICRI	GAGCTC	2 cut(s)	138, 950
EcoO109I	RGGNCCY	4 cut(s)	152, 836, 1411, 4085
EcoO65I	GGTNACC	1 cut(s)	2098
EcoRI	GAATTC	1 cut(s)	2254
EcoRII	CCWGG	7 cut(s)	140, 1389, 1633, 2039, 2320, 3105, 3690
EcoRV	GATATC	1 cut(s)	911
EcoT14I	CCWWGG	3 cut(s)	429, 479, 3943
EcoT22I	ATGCAT	3 cut(s)	601, 1582, 3868
EcoT38I	GRGCYC	3 cut(s)	140, 806, 952
ErhI	CCWWGG	3 cut(s)	429, 479, 3943
FalI	AAGNNNNNCTT	4 cut(s)	413, 445, 3025, 3057
FaqI	GGGAC	2 cut(s)	172, 4071
FauI	CCCGC	2 cut(s)	3842, 3880
FauNDI	CATATG	1 cut(s)	3754
FbaI	TGATCA	1 cut(s)	1342
FokI	GGATG	9 cut(s)	333, 888, 905, 1106, 1265, 1651, 1789, 2168, 3925
FriOI	GRGCYC	3 cut(s)	140, 806, 952
FspBI	CTAG	9 cut(s)	27, 132, 824, 1035, 1350, 2213, 2912, 2990, 3125
GsuI	CTGGAG	3 cut(s)	124, 2186, 3039
HapII	CCGG	9 cut(s)	287, 330, 383, 1615, 1807, 2012, 2090, 2231, 2306
HgaI	GACGC	3 cut(s)	443, 1018, 1283
Hin1I	GRCGYC	2 cut(s)	435, 1275
HincII	GTYRAC	3 cut(s)	634, 855, 2905
HindII	GTYRAC	3 cut(s)	634, 855, 2905
HindIII	AAGCTT	2 cut(s)	469, 1663
HpaI	GTTAAC	1 cut(s)	634
HpaII	CCGG	9 cut(s)	287, 330, 383, 1615, 1807, 2012, 2090, 2231, 2306
HphI	GGTGA	9 cut(s)	39, 566, 961, 1736, 3085, 3307, 3554, 3705, 3822
Hpy166II	GTNNAC	9 cut(s)	518, 634, 855, 2905, 3161, 3412, 3553, 3977, 4094
Hpy188I	TCNGA	9 cut(s)	313, 646, 875, 1319, 1759, 1876, 2194, 2647, 3559
Hpy8I	GTNNAC	9 cut(s)	518, 634, 855, 2905, 3161, 3412, 3553, 3977, 4094
Hpy99I	CGWCG	1 cut(s)	88
HpyAV	CCTTC	8 cut(s)	126, 233, 1856, 2009, 2189, 2662, 3149, 3412
HpyCH4III	ACNGT	7 cut(s)	556, 755, 1286, 1846, 2545, 3073, 3145
HpyCH4IV	ACGT	3 cut(s)	412, 1177, 3499
HpyF10VI	GCNNNNNNNGC	8 cut(s)	434, 1035, 2723, 2811, 3101, 3134, 3470, 3872
HpySE526I	ACGT	3 cut(s)	412, 1177, 3499
Hsp92I	GRCGYC	2 cut(s)	435, 1275
Kpn2I	TCCGGA	2 cut(s)	2089, 2230
KpnI	GGTACC	1 cut(s)	1003
Ksp22I	TGATCA	1 cut(s)	1342
KspAI	GTTAAC	1 cut(s)	634
LguI	GCTCTTC	1 cut(s)	2691
Lsp1109I	GCAGC	9 cut(s)	477, 1574, 1873, 2359, 2608, 2926, 3121, 3616, 3887
LweI	GCATC	7 cut(s)	657, 1451, 1648, 2107, 2892, 3465, 3875
MaeI	CTAG	9 cut(s)	27, 132, 824, 1035, 1350, 2213, 2912, 2990, 3125
MaeII	ACGT	3 cut(s)	412, 1177, 3499
MaeIII	GTNAC	7 cut(s)	399, 967, 1030, 1271, 1795, 2098, 2848
MfeI	CAATTG	1 cut(s)	1894
MflI	RGATCY	6 cut(s)	289, 541, 607, 3119, 3662, 3708
MhlI	GDGCHC	8 cut(s)	140, 806, 952, 2212, 2305, 2816, 3163, 3957
MlsI	TGGCCA	3 cut(s)	2325, 3534, 3948
MluI	ACGCGT	1 cut(s)	1027
MluNI	TGGCCA	3 cut(s)	2325, 3534, 3948
MlyI	GAGTC	4 cut(s)	1954, 2341, 3548, 3890
MmeI	TCCRAC	5 cut(s)	277, 1414, 1450, 1523, 2557
Mox20I	TGGCCA	3 cut(s)	2325, 3534, 3948
Mph1103I	ATGCAT	3 cut(s)	601, 1582, 3868
MroI	TCCGGA	2 cut(s)	2089, 2230
MroXI	GAANNNNTTC	1 cut(s)	1707
MscI	TGGCCA	3 cut(s)	2325, 3534, 3948
MslI	CAYNNNNRTG	6 cut(s)	56, 447, 1373, 2034, 3540, 3960
Msp20I	TGGCCA	3 cut(s)	2325, 3534, 3948
MspA1I	CMGCKG	3 cut(s)	490, 1985, 3578
MspCI	CTTAAG	3 cut(s)	889, 1949, 2452
MspI	CCGG	9 cut(s)	287, 330, 383, 1615, 1807, 2012, 2090, 2231, 2306
MunI	CAATTG	1 cut(s)	1894
Mva1269I	GAATGC	2 cut(s)	2475, 3013
MvaI	CCWGG	7 cut(s)	142, 1391, 1635, 2041, 2322, 3107, 3692
MvnI	CGCG	1 cut(s)	1029
MwoI	GCNNNNNNNGC	8 cut(s)	434, 1035, 2723, 2811, 3101, 3134, 3470, 3872
NciI	CCSGG	5 cut(s)	287, 383, 1807, 2012, 2307
NcoI	CCATGG	2 cut(s)	479, 3943
NdeI	CATATG	1 cut(s)	3754
NmuCI	GTSAC	5 cut(s)	967, 1030, 1271, 1795, 2848
NsiI	ATGCAT	3 cut(s)	601, 1582, 3868
NspI	RCATGY	7 cut(s)	208, 599, 695, 1433, 1749, 1943, 3866
NspV	TTCGAA	2 cut(s)	1465, 1765
OliI	CACNNNNGTG	1 cut(s)	56
PacI	TTAATTAA	1 cut(s)	2178
PaeI	GCATGC	2 cut(s)	695, 1943
PagI	TCATGA	1 cut(s)	3280
PasI	CCCWGGG	1 cut(s)	1634
PceI	AGGCCT	3 cut(s)	1866, 2821, 2873
PciI	ACATGT	2 cut(s)	1429, 1745
PciSI	GCTCTTC	1 cut(s)	2691
PcsI	WCGNNNNNNNCGW	1 cut(s)	3886
PctI	GAATGC	2 cut(s)	2475, 3013
PdmI	GAANNNNTTC	1 cut(s)	1707
PflMI	CCANNNNNTGG	2 cut(s)	277, 1505
PfoI	TCCNGGA	3 cut(s)	140, 381, 3690
PleI	GAGTC	4 cut(s)	1953, 2340, 3548, 3890
PpsI	GAGTC	4 cut(s)	1953, 2340, 3548, 3890
PpuMI	RGGWCCY	4 cut(s)	152, 836, 1411, 4085
PscI	ACATGT	2 cut(s)	1429, 1745
PshBI	ATTAAT	1 cut(s)	2174
Psp124BI	GAGCTC	2 cut(s)	140, 952
Psp5II	RGGWCCY	4 cut(s)	152, 836, 1411, 4085
Psp6I	CCWGG	7 cut(s)	140, 1389, 1633, 2039, 2320, 3105, 3690
PspEI	GGTNACC	1 cut(s)	2098
PspGI	CCWGG	7 cut(s)	140, 1389, 1633, 2039, 2320, 3105, 3690
PspPPI	RGGWCCY	4 cut(s)	152, 836, 1411, 4085
PsrI	GAACNNNNNNTAC	2 cut(s)	510, 542
PstI	CTGCAG	1 cut(s)	151
PstNI	CAGNNNCTG	5 cut(s)	155, 2720, 2774, 2852, 3002
PsuI	RGATCY	6 cut(s)	289, 541, 607, 3119, 3662, 3708
PvuII	CAGCTG	2 cut(s)	490, 3578
RseI	CAYNNNNRTG	6 cut(s)	56, 447, 1373, 2034, 3540, 3960
SacI	GAGCTC	2 cut(s)	140, 952
SapI	GCTCTTC	1 cut(s)	2691
ScaI	AGTACT	4 cut(s)	101, 553, 2511, 3785
SchI	GAGTC	4 cut(s)	1954, 2341, 3548, 3890
SduI	GDGCHC	8 cut(s)	140, 806, 952, 2212, 2305, 2816, 3163, 3957
SfaNI	GCATC	7 cut(s)	657, 1451, 1648, 2107, 2892, 3465, 3875
SfcI	CTRYAG	3 cut(s)	147, 1842, 1851
SfuI	TTCGAA	2 cut(s)	1465, 1765
SmiMI	CAYNNNNRTG	6 cut(s)	56, 447, 1373, 2034, 3540, 3960
SmlI	CTYRAG	6 cut(s)	889, 1949, 2452, 2549, 4036, 4114
SmoI	CTYRAG	6 cut(s)	889, 1949, 2452, 2549, 4036, 4114
SphI	GCATGC	2 cut(s)	695, 1943
SseBI	AGGCCT	3 cut(s)	1866, 2821, 2873
SsiI	CCGC	7 cut(s)	1985, 2241, 2375, 3366, 3835, 3873, 4058
SspI	AATATT	2 cut(s)	1785, 3436
SspMI	CTAG	9 cut(s)	27, 132, 824, 1035, 1350, 2213, 2912, 2990, 3125
SstI	GAGCTC	2 cut(s)	140, 952
StuI	AGGCCT	3 cut(s)	1866, 2821, 2873
StyI	CCWWGG	3 cut(s)	429, 479, 3943
TaaI	ACNGT	7 cut(s)	556, 755, 1286, 1846, 2545, 3073, 3145
TaiI	ACGT	3 cut(s)	415, 1180, 3502
TaqI	TCGA	9 cut(s)	11, 86, 907, 1465, 1650, 1765, 1911, 2629, 3060
TaqII	GACCGA	2 cut(s)	726, 3039
TatI	WGTACW	7 cut(s)	99, 551, 1098, 2460, 2509, 3145, 3783
TauI	GCSGC	2 cut(s)	2243, 3368
TseFI	GTSAC	5 cut(s)	967, 1030, 1271, 1795, 2848
TseI	GCWGC	9 cut(s)	490, 1562, 1886, 2372, 2596, 2939, 3134, 3604, 3875
Tsp45I	GTSAC	5 cut(s)	967, 1030, 1271, 1795, 2848
TspDTI	ATGAA	8 cut(s)	449, 609, 1128, 1929, 2267, 3045, 3165, 3428
TspGWI	ACGGA	2 cut(s)	72, 617
Van91I	CCANNNNNTGG	2 cut(s)	277, 1505
Vha464I	CTTAAG	3 cut(s)	889, 1949, 2452
VneI	GTGCAC	1 cut(s)	3159
VspI	ATTAAT	1 cut(s)	2174
XapI	RAATTY	7 cut(s)	273, 793, 928, 1069, 1760, 2254, 3617
XceI	RCATGY	7 cut(s)	208, 599, 695, 1433, 1749, 1943, 3866
XmnI	GAANNNNTTC	1 cut(s)	1707
XspI	CTAG	9 cut(s)	27, 132, 824, 1035, 1350, 2213, 2912, 2990, 3125
ZrmI	AGTACT	4 cut(s)	101, 553, 2511, 3785
Zsp2I	ATGCAT	3 cut(s)	601, 1582, 3868

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.