Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0008534

Physical Location & Seq

Genomic Coordinates Reverse (-)

25006953 .. 25017063

Gene Structure 10111 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g22840.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4053 bp

ATGCTATTTTGTTCTGATTACTCACCAATGGCAAAATCAAGGCAACATTTCTCTAGCCAAGATTCATCACTGTCCCCAACTTCTGTGCGATCCCAAGAATGGGAGGGTCCATCAAGATGGACTGAATATTTGGGTCCAGAGACAACTTCTCCAATGAATTCGAGGAGCTCCAGGAATTCAGGTCCTGATGAACAGGTCCATAGTTCAGGCGGGTCCCACAAAGGCTTGAACATGCAGTGGGTAGTTCAAGTAACTGAAGTGGCAGAAGGTCTTATGGCTAAAATGTATAGGCTGAACCAAATTTTGGATTACCCGGATCCAGTTGGCCATGTATTTTCAGAAGCATTTTGGAAAGCCGGTGTGTTCCCCAACCATCCAAGAATTTGCTTGTTACTCTCAAAGAAGTTCCCTGAACATTATAGCAAACTACAGCTTGAACGCGTTGATAAGGTTTCTTGGGATGCCTTGCAAGACAATGCAGAACTTCATTTACAAAGCTTGGAACCATGGATCCAGCTGCTTGTTGATTTGATGGCATTCCGAGAACAAGCTCTACGACTCATATTGGATCTGAGCAGCACAGTTATTACCTTGTTGCCCCATCAGAACTCACTTATACTACATGCATTTATGGATCTCTTTTGTTCCTTTGTCCGCGTGAACCTTTTCTCAGAGAAGATGCCAAGGAAAATGATGCTACAAATGTATAACCTTTTGCATTCCATGTCAAGAGACCGAGATTGTGATTTTTATCATCGGTTGGTTCAGTTTATAGACAGTTATGATCCACCGCTGAAAGGATTAAAAGAAGACCTAAATTTTGTGAGCCCACGTATTGGAGAGGTGCTAGAGGCTGTGGGACCTATTATTTTTCTGTCAACAGATACAAGAAAGCTCAGAAATGAAGGTTTTTTAAGTCCATATCATCCTCGATATCCAGACATACTTACAAATTCTTCTCACCCAATGAGAGCTCAGGATCGATCAAATGTCACCTCTTATCGTGAATGGGTATTATTTGGGTATCTTGTTTGTCCTGATGAGCTTCTTCGAGTTACTAGCATTGACATTGCTCTGGTCGTATTAAAAGAAAATCTGGTTCTTACTTTGTTCAGGGATGAGTATATACTTTTGCATGAGGATTATCAGCTATATGTCCTGCCACTGATTTTAGAATCAAAGAAGATGGCAAAGTCTGGTCGTACGAAGCAAAAAGAAGCAGATCTGGAGTATAGTGTAGCTAAGCAAGTTGAGAAAATGATAAGTGAAGTGCATGAGCAAGCATTACTCTCGTGTGATGCCATTCATCATGAGAGGAGAATATTGCTAAAGCAAGAGATTGGAAGAATGGCGCTTTTCTTCACTGATCAGCCTAGCTTGTTGGCTCCAAACATACAAATGGTATTCTCTGCCTTGGCTTTTGCTCAATGTGAGGTCGTCTGGTATTTCCAACATATTGGGATTGTGTCATCAAAATCAAAAACCACTCGAATGGTACCTGTTGACATAGACCCCAGTGATCCAACTATTGGGTTCTTATTAGATGGAATGGACCATCTATGCTGTTTAGTACGCAAGTACATTGCAGCGATTCGAGGTTATGCATTATCATACCTTTCTTCTTGTGCTGTTAGAATCCGGTCTTTGTTGAATACCCCTGGGATGGTGGCTCTTGACTTAGATTCAAGCTTGAAAGGTCTTTTTCAGCAGATTGTTCAGCACCTTGAAAACATACCAAAACCACAGGGTGAAAATGTATCTGCCATTACATGTGATCTAACTGAATTCCGAAAGGATTGGTTATCAATATTGATGATAGTCACATCTTCCCGATCATCTATAAACATAAGACATTTGGAGAAAGCTACAGTTTCTACTGGGAAAGAAGGCTTATTATCGGAAGGAAATGCTGCATACAATTGGTCCAGATGTGTTGACGAACTCGAATCTCAATTATCAAAGCATGCGAGTATTAAGAAGTTGTATTTTTACCATCAGCACCTTACAGCAGTCTTCAGGAACACTATGTTTGGCCCAGAAGGGCGCCCACAGCATTGCTGTGCATGGCTTGGCATTGCAAGTAGTTTCCCAGAATGTGCTTCCCCCATTGTTCCAGAAGAGGTAACTAAAATTGGCAGGGATGCTGTCCTTTATGTTGAATCTCTCATTGAATCAATAATGGGAGGACTCGAGGGATTAATCAACATACTTGATTCTGAAGGAGGATTCGGTGCCCTTGAGATCCAGCTTCTTCCAGAGCAGGCAGCGTATTATATGAATTATGCATCCAGAGTATCAAATCTTTCAGCTAAATCTCCGAAGGGAGCATCTGGTTTCCCTTTTCCTGGTCAGGAGAGTCATCCTGAAAATAATACTTCTATCAAAATGTTAGAAGCTGCAGTGCAGCGACTGACCAATTTATGCTTGGTTTTGAACGATATGGAGCCGATATGTGTGCTAAACCATGTTTTTGTCTTAAGGGAGTACATGAGAGAATGCATCCTTGGGAACCTTAGGAGGAGATTGCTTTCGGCACTGAAAACTGATACTGATCTCCAACGGCCTTCTGTTCTTGAGTCACTTATTCGGAGACACTTAAGCATAATCCATCTGGCAGAGCAGCATATTAGCATGGACCTAACTCAAGGTATTCGAGAAGTTCTGCTCTCAGAGGCCTTCTCAGGACCAGTTTCTTCTTTGCATTTGTTTGAGAAGCCAGCAGAGCAACACACAGGATCAGCCACTGAGGCTGTTTGCAACTGGTACATTGAAAATATAATCAAGGATATATCTGGCGCTGGAATCCTTTTTGCTCCAATTCACAAATGCTTCAAGAGCACGAGGCCTGTTGGTGGATATTTTGCTGATTCAGTCACTGATATCAAAGAATTGAAGGCCTTTGTTCGGATTTTTGGTGGCTATGGAGTTGACAGGTTAGACAGAATGCTGAAAGAACACACTGCTGCCCTTTTGAATTGTATTGACACATCATTGAGATCGAACCGTGAAGTGCTAGAGGCAGTTGCTGGCAGCCTGCATTCTGGTGACCGAAGGGAAAGAGACGCATCAATAAAGCAGATACTCGATATAGACACCGTGATTGGGTTTTGCGTTCAAGCTGGGCTGGCCTTGGCTTTTGACAGACTTCTAGCTGAAGCTTCTGGAGCTGTGCTCTTAGAAGGTGCGCCCTTGATACATTCGTTGTTGACTGGGATAGCTAAGCATATACCTGAAGAAATACCTGAAAAAATAGAAATCAGGAGATTGAGGGGTGTGGCAAACAACTTTGGTGTAGTCTACGATCATGATTCTCAGTGGGTGAGATTTATCTTAGAAGAGGTTGGATGTGCAAACGATGGTTCATGGAGCTTCTTGCCTTATTTATTTGCCACCTTCATGACTTCTAATATTTGGAATACGACTGCCTTCAACGTGGACACAGGGGGATTCAACAACAATATCCACTGCTTGGCAAGGTGCATTACTGCTGTAATTGCAGGAAGTGAGTTTGTTAGACTGGAGCGTGAGCATCAACAAAGACAATCTCTCTCAAATGGTCATGCTGTCGAAACAGGGGACACTGAAATGCAAAGTCGTTCATCAGCTGAGGCAAGCATAAAATCCACAATGCAGCTCTTCGTCAAATTCTCTGCAGGCATTATACTGGATTCATGGAATGAAACTAACCGATCTCATCTTGTTGCACAGCTAATCTTCCTAGACCAACTTTGTGATCGATCTCTCCTTATCTTCCAAGAAGCTCTCTGGAATCCCATGTTCCATACGCCATTCTTCGCTCGATATACAGCCAGTATTACGAAATCTCTCCATCGACACCACTTGCATTACTTAGTGGTTCACCCCGCCATTCACCTGCTGCATCGCTCTCACATTCTTCTCCTGGAGTGCGACATCCCCACGGCGACTCCACTCAGTATGATTCAAGTTATTTCAAGGGATCATCATCCCATGGCCAGGAGTATGATGCTGACAGCGGAAGCTTACGGAGCACAGAAAGCAGGCTGCGGAATGTTCGTCGTTCCGGGCCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005789 GO:0006355 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008150 GO:0009653 GO:0009825 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010026 GO:0010090 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0010638 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0019219 GO:0019222 GO:0030029 GO:0030036 GO:0030154 GO:0030832 GO:0030833 GO:0030838 GO:0031209 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031334 GO:0031984 GO:0032271 GO:0032273 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0032989 GO:0032991 GO:0033043 GO:0040007 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0044087 GO:0044089 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045010 GO:0045893 GO:0045935 GO:0048468 GO:0048518 GO:0048522 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051493 GO:0051495 GO:0060255 GO:0065007 GO:0065008 GO:0071840 GO:0080090 GO:0090066 GO:0090558 GO:0090626 GO:0097435 GO:0098827 GO:0110053 GO:1902680 GO:1902903 GO:1902905 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04810 map04810

Pfam Domains

Protein Families

Nckap1

Protein Analysis

1351

Amino Acids

152.12

Weight (kDa)

6.19

Isoelectric Point (pI)

48.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nckap1	PF09735	85 - 1253	1.2e-290	Nck-associated protein 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0008534)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G35110 AT2G35110
fragaria_vesca	FvH4_3g12410 FvH4_3g12410
malus_domestica	MD05G1250500.v1.1 MD10G1231300.v1.1
prunus_persica	Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1 Prupe.4G113100_v2.0.a1
pyrus_communis	pycom05g22840 pycom10g19450
rosa_chinensis	RchiOBHm_Chr5g0020301
rosa_laevigata	RLG00000032516
rosa_multiflora	Rmu_sc0002087.1_g000004 Rmu_sc0007157.1_g000007
rosa_roxburghii	Rroxscaffold_1G00057910
rosa_rugosa	Rorug05G0058400
rosa_samantha	Rh5AG148800 Rh5BG148400 Rh5CG159900 Rh5DG147200
rosa_wichuraiana	Rw5G013180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	3882
Acc36I	ACCTGC	1 cut(s)	3882
Acc65I	GGTACC	1 cut(s)	1495
AccB1I	GGYRCC	3 cut(s)	1495, 2043, 2231
AccB7I	CCANNNNNTGG	4 cut(s)	304, 836, 1529, 3469
AccI	GTMKAC	1 cut(s)	3297
AccII	CGCG	2 cut(s)	441, 657
AciI	CCGC	6 cut(s)	210, 655, 791, 3864, 3995, 4026
AcoI	YGGCCR	2 cut(s)	325, 3972
AcsI	RAATTY	8 cut(s)	157, 175, 300, 381, 817, 952, 1784, 3644
AcuI	CTGAAG	5 cut(s)	276, 1999, 2238, 3174, 3252
AcyI	GRCGYC	1 cut(s)	2044
AdeI	CACNNNGTG	1 cut(s)	1748
AfaI	GTAC	6 cut(s)	1204, 1497, 1572, 1580, 2486, 2765
AflII	CTTAAG	2 cut(s)	2476, 2596
AflIII	ACRYGT	2 cut(s)	439, 1769
AjnI	CCWGG	5 cut(s)	170, 1657, 2344, 3900, 3974
AjuI	GAANNNNNNNTTGG	4 cut(s)	287, 319, 2487, 2519
AloI	GAACNNNNNNTCC	2 cut(s)	495, 527
Alw21I	GWGCWC	5 cut(s)	170, 976, 2840, 3174, 4012
Alw26I	GTCTC	4 cut(s)	134, 726, 2584, 3054
AlwNI	CAGNNNCTG	5 cut(s)	185, 2410, 2744, 2876, 3026
Ama87I	CYCGRG	1 cut(s)	2189
AoxI	GGCC	9 cut(s)	325, 2032, 2561, 2673, 2843, 2895, 3126, 3972, 4045
ApoI	RAATTY	8 cut(s)	157, 175, 300, 381, 817, 952, 1784, 3644
ArsI	GACNNNNNNTTYG	4 cut(s)	112, 144, 803, 835
AseI	ATTAAT	1 cut(s)	2198
Asp700I	GAANNNNTTC	1 cut(s)	665
Asp718I	GGTACC	1 cut(s)	1495
AspLEI	GCGC	4 cut(s)	1354, 2046, 2798, 3187
AsuC2I	CCSGG	2 cut(s)	314, 4044
AsuHPI	GGTGA	8 cut(s)	15, 953, 985, 1760, 3056, 3331, 3851, 3863
AvaI	CYCGRG	1 cut(s)	2189
AxyI	CCTNAGG	1 cut(s)	2513
BaeGI	GKGCMC	1 cut(s)	2236
BaeI	ACNNNNGTAYC	2 cut(s)	3185, 3218
BalI	TGGCCA	2 cut(s)	327, 3974
BamHI	GGATCC	2 cut(s)	316, 510
BanI	GGYRCC	3 cut(s)	1495, 2043, 2231
BanII	GRGCYC	3 cut(s)	170, 830, 976
BauI	CACGAG	2 cut(s)	1291, 2839
BbsI	GAAGAC	2 cut(s)	816, 2005
Bbv12I	GWGCWC	5 cut(s)	170, 976, 2840, 3174, 4012
BbvCI	CCTCAGC	1 cut(s)	3606
BceAI	ACGGC	2 cut(s)	2576, 3936
BcgI	CGANNNNNNTGC	4 cut(s)	1272, 1306, 2437, 2471
BciT130I	CCWGG	5 cut(s)	172, 1659, 2346, 3902, 3976
BclI	TGATCA	1 cut(s)	1366
BcnI	CCSGG	2 cut(s)	314, 4044
BcoDI	GTCTC	4 cut(s)	134, 726, 2584, 3054
BfaI	CTAG	7 cut(s)	54, 848, 1059, 1374, 3014, 3149, 3719
BfmI	CTRYAG	4 cut(s)	428, 1866, 2397, 3651
BfoI	RGCGCY	3 cut(s)	1355, 2047, 2799
BfrI	CTTAAG	2 cut(s)	2476, 2596
BfuAI	ACCTGC	1 cut(s)	3882
BglI	GCCNNNNNGGC	1 cut(s)	2747
BglII	AGATCT	1 cut(s)	1222
BlpI	GCTNAGC	2 cut(s)	1242, 3219
Bme1390I	CCNGG	7 cut(s)	172, 314, 1659, 2346, 3902, 3976, 4044
BmeT110I	CYCGRG	1 cut(s)	2189
BmrFI	CCNGG	7 cut(s)	172, 314, 1659, 2346, 3902, 3976, 4044
BmrI	ACTGGG	3 cut(s)	1509, 1887, 3219
BmuI	ACTGGG	3 cut(s)	1509, 1887, 3219
BpiI	GAAGAC	2 cut(s)	816, 2005
BplI	GAGNNNNNCTC	4 cut(s)	2663, 2695, 3156, 3188
BpmI	CTGGAG	5 cut(s)	154, 1247, 3183, 3539, 3923
Bpu10I	CCTNAGC	2 cut(s)	975, 3606
Bpu1102I	GCTNAGC	2 cut(s)	1242, 3219
BpuEI	CTTGAG	3 cut(s)	2258, 2594, 2628
BpuMI	CCSGG	2 cut(s)	314, 4044
Bsa29I	ATCGAT	2 cut(s)	982, 3736
BsaAI	YACGTR	1 cut(s)	833
BsaBI	GATNNNNATC	3 cut(s)	750, 2550, 3963
BsaHI	GRCGYC	1 cut(s)	2044
BsaI	GGTCTC	1 cut(s)	726
BsaJI	CCNNGG	9 cut(s)	506, 683, 1413, 1657, 1658, 2503, 3129, 3918, 3969
BsaWI	WCCGGW	1 cut(s)	1638
BsaXI	ACNNNNNCTCC	4 cut(s)	133, 163, 3910, 3940
Bse118I	RCCGGY	1 cut(s)	356
Bse1I	ACTGG	9 cut(s)	320, 1515, 1882, 2687, 2765, 3214, 3522, 3669, 3810
Bse21I	CCTNAGG	1 cut(s)	2513
Bse3DI	GCAATG	4 cut(s)	1068, 1581, 2053, 2073
Bse8I	GATNNNNATC	3 cut(s)	750, 2550, 3963
BseBI	CCWGG	5 cut(s)	172, 1659, 2346, 3902, 3976
BseCI	ATCGAT	2 cut(s)	982, 3736
BseDI	CCNNGG	9 cut(s)	506, 683, 1413, 1657, 1658, 2503, 3129, 3918, 3969
BseJI	GATNNNNATC	3 cut(s)	750, 2550, 3963
BseMI	GCAATG	4 cut(s)	1068, 1581, 2053, 2073
BseNI	ACTGG	9 cut(s)	320, 1515, 1882, 2687, 2765, 3214, 3522, 3669, 3810
BseRI	GAGGAG	3 cut(s)	178, 1330, 2533
BseSI	GKGCMC	1 cut(s)	2236
BseYI	CCCAGC	1 cut(s)	3119
BsgI	GTGCAG	1 cut(s)	2423
Bsh1236I	CGCG	2 cut(s)	441, 657
BshFI	GGCC	9 cut(s)	327, 2034, 2563, 2675, 2845, 2897, 3128, 3974, 4047
BshNI	GGYRCC	3 cut(s)	1495, 2043, 2231
BshVI	ATCGAT	2 cut(s)	982, 3736
BsiHKAI	GWGCWC	5 cut(s)	170, 976, 2840, 3174, 4012
BsiHKCI	CYCGRG	1 cut(s)	2189
BsiSI	CCGG	4 cut(s)	314, 357, 1639, 4043
BsiWI	CGTACG	1 cut(s)	1202
BslFI	GGGAC	4 cut(s)	58, 199, 873, 3590
BsmAI	GTCTC	4 cut(s)	134, 726, 2584, 3054
BsmBI	CGTCTC	1 cut(s)	3054
BsmFI	GGGAC	4 cut(s)	58, 199, 873, 3590
BsmI	GAATGC	5 cut(s)	536, 718, 2501, 2949, 3037
BsnI	GGCC	9 cut(s)	327, 2034, 2563, 2675, 2845, 2897, 3128, 3974, 4047
Bso31I	GGTCTC	1 cut(s)	726
BsoBI	CYCGRG	1 cut(s)	2189
Bsp1286I	GDGCHC	7 cut(s)	170, 830, 976, 2236, 2840, 3174, 4012
Bsp1720I	GCTNAGC	2 cut(s)	1242, 3219
Bsp19I	CCATGG	2 cut(s)	506, 3969
BspACI	CCGC	6 cut(s)	210, 655, 791, 3864, 3995, 4026
BspANI	GGCC	9 cut(s)	327, 2034, 2563, 2675, 2845, 2897, 3128, 3974, 4047
BspDI	ATCGAT	2 cut(s)	982, 3736
BspFNI	CGCG	2 cut(s)	441, 657
BspHI	TCATGA	3 cut(s)	1309, 3304, 3396
BspMAI	CTGCAG	2 cut(s)	2401, 3655
BspMI	ACCTGC	1 cut(s)	3882
BspQI	GCTCTTC	1 cut(s)	3641
BspT107I	GGYRCC	3 cut(s)	1495, 2043, 2231
BspTI	CTTAAG	2 cut(s)	2476, 2596
BspTNI	GGTCTC	1 cut(s)	726
BsrDI	GCAATG	4 cut(s)	1068, 1581, 2053, 2073
BsrFI	RCCGGY	1 cut(s)	356
BsrI	ACTGG	9 cut(s)	320, 1515, 1882, 2687, 2765, 3214, 3522, 3669, 3810
BssAI	RCCGGY	1 cut(s)	356
BssECI	CCNNGG	9 cut(s)	506, 683, 1413, 1657, 1658, 2503, 3129, 3918, 3969
BssNI	GRCGYC	1 cut(s)	2044
BssSI	CACGAG	2 cut(s)	1291, 2839
BssT1I	CCWWGG	6 cut(s)	506, 683, 1413, 2503, 3129, 3969
Bst2BI	CACGAG	2 cut(s)	1291, 2839
Bst2UI	CCWGG	5 cut(s)	172, 1659, 2346, 3902, 3976
Bst4CI	ACNGT	6 cut(s)	72, 583, 779, 1870, 3005, 3097
Bst6I	CTCTTC	3 cut(s)	2112, 3330, 3641
BstACI	GRCGYC	1 cut(s)	2044
BstAFI	CTTAAG	2 cut(s)	2476, 2596
BstBAI	YACGTR	1 cut(s)	833
BstDSI	CCRYGG	3 cut(s)	506, 3918, 3969
BstEII	GGTNACC	1 cut(s)	3044
BstFNI	CGCG	2 cut(s)	441, 657
BstH2I	RGCGCY	3 cut(s)	1355, 2047, 2799
BstHHI	GCGC	4 cut(s)	1354, 2046, 2798, 3187
BstMAI	GTCTC	4 cut(s)	134, 726, 2584, 3054
BstMWI	GCNNNNNNNGC	9 cut(s)	2050, 2722, 2747, 2835, 3125, 3164, 3494, 4007, 4016
BstNI	CCWGG	5 cut(s)	172, 1659, 2346, 3902, 3976
BstNSI	RCATGY	4 cut(s)	235, 626, 1773, 1967
BstPI	GGTNACC	1 cut(s)	3044
BstSCI	CCNGG	7 cut(s)	170, 312, 1657, 2344, 3900, 3974, 4042
BstSFI	CTRYAG	4 cut(s)	428, 1866, 2397, 3651
BstSLI	GKGCMC	1 cut(s)	2236
BstUI	CGCG	2 cut(s)	441, 657
BstV2I	GAAGAC	2 cut(s)	816, 2005
BstX2I	RGATCY	6 cut(s)	316, 510, 568, 634, 1222, 2241
BstXI	CCANNNNNNTGG	3 cut(s)	117, 1457, 1492
BstYI	RGATCY	6 cut(s)	316, 510, 568, 634, 1222, 2241
Bsu15I	ATCGAT	2 cut(s)	982, 3736
Bsu36I	CCTNAGG	1 cut(s)	2513
BsuRI	GGCC	9 cut(s)	327, 2034, 2563, 2675, 2845, 2897, 3128, 3974, 4047
BsuTUI	ATCGAT	2 cut(s)	982, 3736
BtgI	CCRYGG	3 cut(s)	506, 3918, 3969
BtgZI	GCGATG	1 cut(s)	3866
BtsI	GCAGTG	4 cut(s)	242, 2406, 2958, 3463
BveI	ACCTGC	1 cut(s)	3882
CaiI	CAGNNNCTG	5 cut(s)	185, 2410, 2744, 2876, 3026
CciI	TCATGA	3 cut(s)	1309, 3304, 3396
CfoI	GCGC	4 cut(s)	1354, 2046, 2798, 3187
Cfr10I	RCCGGY	1 cut(s)	356
ClaI	ATCGAT	2 cut(s)	982, 3736
CseI	GACGC	1 cut(s)	3071
Csp6I	GTAC	6 cut(s)	1203, 1496, 1571, 1579, 2485, 2764
CviQI	GTAC	6 cut(s)	1203, 1496, 1571, 1579, 2485, 2764
DinI	GGCGCC	1 cut(s)	2045
DraIII	CACNNNGTG	1 cut(s)	1748
EaeI	YGGCCR	2 cut(s)	325, 3972
Eam1104I	CTCTTC	3 cut(s)	2112, 3330, 3641
EarI	CTCTTC	3 cut(s)	2112, 3330, 3641
Ecl136II	GAGCTC	2 cut(s)	168, 974
Eco130I	CCWWGG	6 cut(s)	506, 683, 1413, 2503, 3129, 3969
Eco147I	AGGCCT	3 cut(s)	2675, 2845, 2897
Eco24I	GRGCYC	3 cut(s)	170, 830, 976
Eco31I	GGTCTC	1 cut(s)	726
Eco32I	GATATC	2 cut(s)	935, 2881
Eco53kI	GAGCTC	2 cut(s)	168, 974
Eco57I	CTGAAG	5 cut(s)	276, 1999, 2238, 3174, 3252
Eco81I	CCTNAGG	1 cut(s)	2513
Eco88I	CYCGRG	1 cut(s)	2189
Eco91I	GGTNACC	1 cut(s)	3044
EcoICRI	GAGCTC	2 cut(s)	168, 974
EcoO109I	RGGNCCY	3 cut(s)	182, 213, 860
EcoO65I	GGTNACC	1 cut(s)	3044
EcoRI	GAATTC	3 cut(s)	157, 175, 1784
EcoRII	CCWGG	5 cut(s)	170, 1657, 2344, 3900, 3974
EcoRV	GATATC	2 cut(s)	935, 2881
EcoT14I	CCWWGG	6 cut(s)	506, 683, 1413, 2503, 3129, 3969
EcoT22I	ATGCAT	4 cut(s)	628, 1606, 2287, 2501
EcoT38I	GRGCYC	3 cut(s)	170, 830, 976
EgeI	GGCGCC	1 cut(s)	2045
EheI	GGCGCC	1 cut(s)	2045
ErhI	CCWWGG	6 cut(s)	506, 683, 1413, 2503, 3129, 3969
Esp3I	CGTCTC	1 cut(s)	3054
FaqI	GGGAC	4 cut(s)	58, 199, 873, 3590
FauI	CCCGC	2 cut(s)	203, 3871
FbaI	TGATCA	1 cut(s)	1366
FblI	GTMKAC	1 cut(s)	3297
FriOI	GRGCYC	3 cut(s)	170, 830, 976
FspBI	CTAG	7 cut(s)	54, 848, 1059, 1374, 3014, 3149, 3719
GlaI	GCGC	4 cut(s)	1353, 2045, 2797, 3186
GsaI	CCCAGC	1 cut(s)	3123
GsuI	CTGGAG	5 cut(s)	154, 1247, 3183, 3539, 3923
HaeII	RGCGCY	3 cut(s)	1355, 2047, 2799
HaeIII	GGCC	9 cut(s)	327, 2034, 2563, 2675, 2845, 2897, 3128, 3974, 4047
HapII	CCGG	4 cut(s)	314, 357, 1639, 4043
HgaI	GACGC	1 cut(s)	3071
HhaI	GCGC	4 cut(s)	1354, 2046, 2798, 3187
Hin1I	GRCGYC	1 cut(s)	2044
Hin6I	GCGC	4 cut(s)	1352, 2044, 2796, 3185
HinP1I	GCGC	4 cut(s)	1352, 2044, 2796, 3185
HincII	GTYRAC	5 cut(s)	879, 1504, 1936, 2929, 3207
HindII	GTYRAC	5 cut(s)	879, 1504, 1936, 2929, 3207
HindIII	AAGCTT	4 cut(s)	496, 1687, 3156, 3999
HpaII	CCGG	4 cut(s)	314, 357, 1639, 4043
HphI	GGTGA	8 cut(s)	15, 953, 985, 1760, 3056, 3331, 3851, 3863
Hpy166II	GTNNAC	9 cut(s)	661, 879, 1504, 1936, 2929, 3207, 3298, 3436, 3859
Hpy8I	GTNNAC	9 cut(s)	661, 879, 1504, 1936, 2929, 3207, 3298, 3436, 3859
Hpy99I	CGWCG	1 cut(s)	4040
HpyCH4III	ACNGT	6 cut(s)	72, 583, 779, 1870, 3005, 3097
HpyCH4IV	ACGT	2 cut(s)	832, 3432
HpyF10VI	GCNNNNNNNGC	9 cut(s)	2050, 2722, 2747, 2835, 3125, 3164, 3494, 4007, 4016
HpySE526I	ACGT	2 cut(s)	832, 3432
Hsp92I	GRCGYC	1 cut(s)	2044
HspAI	GCGC	4 cut(s)	1352, 2044, 2796, 3185
KasI	GGCGCC	1 cut(s)	2043
KflI	GGGWCCC	1 cut(s)	213
KpnI	GGTACC	1 cut(s)	1499
Ksp22I	TGATCA	1 cut(s)	1366
LguI	GCTCTTC	1 cut(s)	3641
MaeI	CTAG	7 cut(s)	54, 848, 1059, 1374, 3014, 3149, 3719
MaeII	ACGT	2 cut(s)	832, 3432
MaeIII	GTNAC	9 cut(s)	250, 390, 991, 1054, 1819, 2122, 2577, 2872, 3044
MfeI	CAATTG	1 cut(s)	1918
MflI	RGATCY	6 cut(s)	316, 510, 568, 634, 1222, 2241
MhlI	GDGCHC	7 cut(s)	170, 830, 976, 2236, 2840, 3174, 4012
MlsI	TGGCCA	2 cut(s)	327, 3974
MluI	ACGCGT	1 cut(s)	439
MluNI	TGGCCA	2 cut(s)	327, 3974
Mly113I	GGCGCC	1 cut(s)	2044
MlyI	GAGTC	5 cut(s)	552, 2181, 2365, 2585, 3919
MmeI	TCCRAC	4 cut(s)	1474, 1547, 2581, 3322
Mox20I	TGGCCA	2 cut(s)	327, 3974
Mph1103I	ATGCAT	4 cut(s)	628, 1606, 2287, 2501
MroXI	GAANNNNTTC	1 cut(s)	665
MscI	TGGCCA	2 cut(s)	327, 3974
MseI	TTAA	7 cut(s)	803, 914, 1085, 1974, 2198, 2477, 2597
MslI	CAYNNNNRTG	7 cut(s)	115, 1397, 1490, 2058, 3042, 3395, 3584
Msp20I	TGGCCA	2 cut(s)	327, 3974
MspA1I	CMGCKG	4 cut(s)	517, 793, 3605, 3995
MspCI	CTTAAG	2 cut(s)	2476, 2596
MspI	CCGG	4 cut(s)	314, 357, 1639, 4043
MspR9I	CCNGG	7 cut(s)	172, 314, 1659, 2346, 3902, 3976, 4044
MunI	CAATTG	1 cut(s)	1918
Mva1269I	GAATGC	5 cut(s)	536, 718, 2501, 2949, 3037
MvaI	CCWGG	5 cut(s)	172, 1659, 2346, 3902, 3976
MvnI	CGCG	2 cut(s)	441, 657
MwoI	GCNNNNNNNGC	9 cut(s)	2050, 2722, 2747, 2835, 3125, 3164, 3494, 4007, 4016
NarI	GGCGCC	1 cut(s)	2044
NciI	CCSGG	2 cut(s)	314, 4044
NcoI	CCATGG	2 cut(s)	506, 3969
NmuCI	GTSAC	5 cut(s)	991, 1819, 2577, 2872, 3044
NsiI	ATGCAT	4 cut(s)	628, 1606, 2287, 2501
NspI	RCATGY	4 cut(s)	235, 626, 1773, 1967
PaeI	GCATGC	1 cut(s)	1967
PaeR7I	CTCGAG	1 cut(s)	2189
PagI	TCATGA	3 cut(s)	1309, 3304, 3396
PaqCI	CACCTGC	1 cut(s)	3882
PasI	CCCWGGG	1 cut(s)	1658
PceI	AGGCCT	3 cut(s)	2675, 2845, 2897
PciI	ACATGT	1 cut(s)	1769
PciSI	GCTCTTC	1 cut(s)	3641
PctI	GAATGC	5 cut(s)	536, 718, 2501, 2949, 3037
PdmI	GAANNNNTTC	1 cut(s)	665
Pfl23II	CGTACG	1 cut(s)	1202
PflMI	CCANNNNNTGG	4 cut(s)	304, 836, 1529, 3469
PfoI	TCCNGGA	2 cut(s)	170, 3900
PleI	GAGTC	5 cut(s)	552, 2181, 2364, 2584, 3919
PluTI	GGCGCC	1 cut(s)	2047
PpsI	GAGTC	5 cut(s)	552, 2181, 2364, 2584, 3919
Ppu21I	YACGTR	1 cut(s)	833
PpuMI	RGGWCCY	3 cut(s)	182, 213, 860
PscI	ACATGT	1 cut(s)	1769
PshBI	ATTAAT	1 cut(s)	2198
Psp124BI	GAGCTC	2 cut(s)	170, 976
Psp5II	RGGWCCY	3 cut(s)	182, 213, 860
Psp6I	CCWGG	5 cut(s)	170, 1657, 2344, 3900, 3974
PspEI	GGTNACC	1 cut(s)	3044
PspFI	CCCAGC	1 cut(s)	3119
PspGI	CCWGG	5 cut(s)	170, 1657, 2344, 3900, 3974
PspLI	CGTACG	1 cut(s)	1202
PspPPI	RGGWCCY	3 cut(s)	182, 213, 860
PspXI	VCTCGAGB	1 cut(s)	2189
PsrI	GAACNNNNNNTAC	4 cut(s)	474, 506, 537, 569
PstI	CTGCAG	2 cut(s)	2401, 3655
PstNI	CAGNNNCTG	5 cut(s)	185, 2410, 2744, 2876, 3026
PsuI	RGATCY	6 cut(s)	316, 510, 568, 634, 1222, 2241
PvuII	CAGCTG	2 cut(s)	517, 3605
RsaI	GTAC	6 cut(s)	1204, 1497, 1572, 1580, 2486, 2765
RsaNI	GTAC	6 cut(s)	1203, 1496, 1571, 1579, 2485, 2764
RseI	CAYNNNNRTG	7 cut(s)	115, 1397, 1490, 2058, 3042, 3395, 3584
SacI	GAGCTC	2 cut(s)	170, 976
SapI	GCTCTTC	1 cut(s)	3641
SaqAI	TTAA	7 cut(s)	803, 914, 1085, 1974, 2198, 2477, 2597
SchI	GAGTC	5 cut(s)	552, 2181, 2365, 2585, 3919
ScrFI	CCNGG	7 cut(s)	172, 314, 1659, 2346, 3902, 3976, 4044
SduI	GDGCHC	7 cut(s)	170, 830, 976, 2236, 2840, 3174, 4012
SfcI	CTRYAG	4 cut(s)	428, 1866, 2397, 3651
SfoI	GGCGCC	1 cut(s)	2045
Sfr274I	CTCGAG	1 cut(s)	2189
SlaI	CTCGAG	1 cut(s)	2189
SmiMI	CAYNNNNRTG	7 cut(s)	115, 1397, 1490, 2058, 3042, 3395, 3584
SmlI	CTYRAG	6 cut(s)	2189, 2237, 2476, 2573, 2596, 2643
SmoI	CTYRAG	6 cut(s)	2189, 2237, 2476, 2573, 2596, 2643
SphI	GCATGC	1 cut(s)	1967
SseBI	AGGCCT	3 cut(s)	2675, 2845, 2897
SsiI	CCGC	6 cut(s)	210, 655, 791, 3864, 3995, 4026
SspDI	GGCGCC	1 cut(s)	2043
SspI	AATATT	4 cut(s)	128, 1323, 1809, 3409
SspMI	CTAG	7 cut(s)	54, 848, 1059, 1374, 3014, 3149, 3719
SstI	GAGCTC	2 cut(s)	170, 976
StuI	AGGCCT	3 cut(s)	2675, 2845, 2897
StyD4I	CCNGG	7 cut(s)	170, 312, 1657, 2344, 3900, 3974, 4042
StyI	CCWWGG	6 cut(s)	506, 683, 1413, 2503, 3129, 3969
TaaI	ACNGT	6 cut(s)	72, 583, 779, 1870, 3005, 3097
TaiI	ACGT	2 cut(s)	835, 3435
TaqII	GACCGA	2 cut(s)	750, 3063
TatI	WGTACW	2 cut(s)	1578, 2484
Tru1I	TTAA	7 cut(s)	803, 914, 1085, 1974, 2198, 2477, 2597
Tru9I	TTAA	7 cut(s)	803, 914, 1085, 1974, 2198, 2477, 2597
TseFI	GTSAC	5 cut(s)	991, 1819, 2577, 2872, 3044
Tsp45I	GTSAC	5 cut(s)	991, 1819, 2577, 2872, 3044
TspGWI	ACGGA	1 cut(s)	4020
Van91I	CCANNNNNTGG	4 cut(s)	304, 836, 1529, 3469
Vha464I	CTTAAG	2 cut(s)	2476, 2596
VspI	ATTAAT	1 cut(s)	2198
XapI	RAATTY	8 cut(s)	157, 175, 300, 381, 817, 952, 1784, 3644
XceI	RCATGY	4 cut(s)	235, 626, 1773, 1967
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2422
XhoI	CTCGAG	1 cut(s)	2189
XmiI	GTMKAC	1 cut(s)	3297
XmnI	GAANNNNTTC	1 cut(s)	665
XspI	CTAG	7 cut(s)	54, 848, 1059, 1374, 3014, 3149, 3719
Zsp2I	ATGCAT	4 cut(s)	628, 1606, 2287, 2501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.