AT2G40780

RNA-binding protein EIF1AD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0013252
Physical Location & Seq
Forward (+)
17021505 .. 17022944
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40780.1

Sequence Viewer

Length: 516 bp
ATGAACAGAGGAAGAAGGAATCTGAAACAAGCGGCGTCGGACCAGGATTTCACGCTTGAGGAATGTCAGAGCATTGCCCAAGTCGTCTCTCTCAGAGGTTCCAATCAAATTGAGATAATGGATGCAAAAGGAGAGAACTCATTAGCTTTGTTTCCAGCCAAGTTTCGTGAGAGCATGTGGATCAGACGAGGAAGCTTTGTAGTGATTGACCATACAGGAAAGGAAAAGGCTCAAGAGTCTGGTAGCAAAGTTACATCTATTGTATGTAAAGTTCTATTCTTTGAGCAAGTCCGTCTTCTTCAAAAGTCTCCGGAATGGCCAGAAATCTTCAAAGATACTAGACCGATTCCAGCTGAGAAAAGCTCACCCATTGAACAGCATGAAGATGACGGTGAAGTTGATTCGAGTGATGATGATGATGGTATGCCTCCATTGCAAGCAAACACAAACAGATTGAGACCGTTTGGGGTGAAGTGTGATGCAGAAACTGATTCAGGGTCAGATTCCGATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

171

Amino Acids

19.11

Weight (kDa)

4.8

Isoelectric Point (pI)

58.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 23 - 74 5.3e-11 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013252)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40780 AT2G40780
fragaria_vesca FvH4_4g04970 FvH4_7g26120
malus_domestica MD01G1169400.v1.1
prunus_persica Prupe.2G265600_v2.0.a1
pyrus_communis pycom01g18210 pycom07g21280
rosa_chinensis RchiOBHm_Chr1g0372561
rosa_laevigata RLG00000026891
rosa_multiflora Rmu_co8142570.1_g000001 Rmu_sc0011709.1_g000001
rosa_roxburghii Rroxscaffold_4G00284850
rosa_rugosa Rorug01G0372800
rosa_samantha Rh1AG381300 Rh1BG344700 Rh1CG358100
rosa_wichuraiana Rw1G033530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 310
AciI CCGC 1 cut(s) 32
AclWI GGATC 1 cut(s) 188
AcoI YGGCCR 1 cut(s) 317
AcyI GRCGYC 1 cut(s) 35
AgsI TTSAA 3 cut(s) 302, 331, 374
AjnI CCWGG 1 cut(s) 42
AluBI AGCT 4 cut(s) 146, 195, 353, 363
AluI AGCT 4 cut(s) 146, 195, 353, 363
Alw26I GTCTC 3 cut(s) 91, 312, 451
AlwI GGATC 1 cut(s) 188
AlwNI CAGNNNCTG 1 cut(s) 488
Aor13HI TCCGGA 1 cut(s) 310
AoxI GGCC 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 40
AsuHPI GGTGA 3 cut(s) 357, 404, 481
AvaII GGWCC 1 cut(s) 40
BalI TGGCCA 1 cut(s) 319
BbsI GAAGAC 1 cut(s) 287
BccI CCATC 1 cut(s) 413
BciT130I CCWGG 1 cut(s) 44
BcoDI GTCTC 3 cut(s) 91, 312, 451
BfaI CTAG 1 cut(s) 339
BisI GCNGC 1 cut(s) 33
BlsI GCNGC 1 cut(s) 34
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 1 cut(s) 40
BmgT120I GGNCC 1 cut(s) 40
BmiI GGNNCC 1 cut(s) 100
BmrFI CCNGG 1 cut(s) 44
BmsI GCATC 2 cut(s) 112, 469
BpiI GAAGAC 1 cut(s) 287
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpuEI CTTGAG 2 cut(s) 77, 216
BsaHI GRCGYC 1 cut(s) 35
BsaI GGTCTC 1 cut(s) 451
BsaWI WCCGGW 1 cut(s) 310
Bse3DI GCAATG 2 cut(s) 72, 431
BseAI TCCGGA 1 cut(s) 310
BseBI CCWGG 1 cut(s) 44
BseGI GGATG 1 cut(s) 127
BseMI GCAATG 2 cut(s) 72, 431
BseMII CTCAG 2 cut(s) 106, 345
BshFI GGCC 1 cut(s) 319
BsiSI CCGG 1 cut(s) 311
BsmAI GTCTC 3 cut(s) 91, 312, 451
BsmBI CGTCTC 1 cut(s) 91
BsnI GGCC 1 cut(s) 319
Bso31I GGTCTC 1 cut(s) 451
Bsp13I TCCGGA 1 cut(s) 310
Bsp143I GATC 1 cut(s) 180
BspACI CCGC 1 cut(s) 32
BspANI GGCC 1 cut(s) 319
BspCNI CTCAG 2 cut(s) 105, 346
BspEI TCCGGA 1 cut(s) 310
BspLI GGNNCC 1 cut(s) 100
BspPI GGATC 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 451
BsrDI GCAATG 2 cut(s) 72, 431
BssMI GATC 1 cut(s) 180
BssNI GRCGYC 1 cut(s) 35
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 2 cut(s) 392, 462
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 1 cut(s) 438
BstDEI CTNAG 2 cut(s) 92, 354
BstF5I GGATG 1 cut(s) 127
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 3 cut(s) 91, 312, 451
BstMBI GATC 1 cut(s) 180
BstMWI GCNNNNNNNGC 1 cut(s) 433
BstNI CCWGG 1 cut(s) 44
BstNSI RCATGY 1 cut(s) 178
BstSCI CCNGG 1 cut(s) 42
BstV2I GAAGAC 1 cut(s) 287
BsuRI GGCC 1 cut(s) 319
BtsCI GGATG 1 cut(s) 127
Cac8I GCNNGC 1 cut(s) 438
CaiI CAGNNNCTG 1 cut(s) 488
Cfr13I GGNCC 1 cut(s) 40
CseI GACGC 1 cut(s) 24
CviAII CATG 2 cut(s) 175, 380
CviJI RGCY 7 cut(s) 146, 158, 195, 230, 319, 353, 363
CviKI_1 RGCY 7 cut(s) 146, 158, 195, 230, 319, 353, 363
DdeI CTNAG 2 cut(s) 92, 354
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
EaeI YGGCCR 1 cut(s) 317
Eco31I GGTCTC 1 cut(s) 451
Eco47I GGWCC 1 cut(s) 40
EcoRII CCWGG 1 cut(s) 42
Esp3I CGTCTC 1 cut(s) 91
FaeI CATG 2 cut(s) 178, 383
FaiI YATR 6 cut(s) 176, 213, 265, 381, 425, 514
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FatI CATG 2 cut(s) 174, 379
Fnu4HI GCNGC 1 cut(s) 33
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 1 cut(s) 33
FspBI CTAG 1 cut(s) 339
GluI GCNGC 1 cut(s) 33
HaeIII GGCC 1 cut(s) 319
HapII CCGG 1 cut(s) 311
HgaI GACGC 1 cut(s) 24
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 2 cut(s) 178, 383
HindIII AAGCTT 1 cut(s) 193
HinfI GANTC 7 cut(s) 19, 236, 346, 401, 491, 503, 509
HpaII CCGG 1 cut(s) 311
HphI GGTGA 3 cut(s) 357, 404, 481
Hpy188I TCNGA 7 cut(s) 24, 40, 69, 95, 185, 502, 508
Hpy188III TCNNGA 3 cut(s) 167, 233, 311
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 1 cut(s) 9
HpyCH4III ACNGT 2 cut(s) 392, 462
HpyCH4V TGCA 3 cut(s) 125, 436, 482
HpyF10VI GCNNNNNNNGC 1 cut(s) 433
HpyF3I CTNAG 2 cut(s) 92, 354
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 2 cut(s) 178, 383
Kpn2I TCCGGA 1 cut(s) 310
Kzo9I GATC 1 cut(s) 180
LpnPI CCDG 9 cut(s) 29, 56, 168, 201, 225, 324, 333, 363, 480
LweI GCATC 2 cut(s) 112, 469
MaeI CTAG 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 250
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 5 cut(s) 24, 287, 290, 319, 395
MlsI TGGCCA 1 cut(s) 319
MluCI AATT 1 cut(s) 108
MluNI TGGCCA 1 cut(s) 319
MlyI GAGTC 1 cut(s) 245
MmeI TCCRAC 1 cut(s) 18
MnlI CCTC 4 cut(s) 52, 89, 182, 438
Mox20I TGGCCA 1 cut(s) 319
MroI TCCGGA 1 cut(s) 310
MscI TGGCCA 1 cut(s) 319
MslI CAYNNNNRTG 1 cut(s) 384
Msp20I TGGCCA 1 cut(s) 319
MspA1I CMGCKG 1 cut(s) 353
MspI CCGG 1 cut(s) 311
MspR9I CCNGG 1 cut(s) 44
MvaI CCWGG 1 cut(s) 44
MwoI GCNNNNNNNGC 1 cut(s) 433
NdeII GATC 1 cut(s) 180
NlaIII CATG 2 cut(s) 178, 383
NlaIV GGNNCC 1 cut(s) 100
NspI RCATGY 1 cut(s) 178
PfeI GAWTC 6 cut(s) 19, 346, 401, 491, 503, 509
PkrI GCNGC 1 cut(s) 34
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
Psp6I CCWGG 1 cut(s) 42
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 1 cut(s) 100
PspPI GGNCC 1 cut(s) 40
PsrI GAACNNNNNNTAC 2 cut(s) 255, 287
PstNI CAGNNNCTG 1 cut(s) 488
PvuII CAGCTG 1 cut(s) 353
RseI CAYNNNNRTG 1 cut(s) 384
SatI GCNGC 1 cut(s) 33
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 1 cut(s) 40
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 1 cut(s) 44
SetI ASST 5 cut(s) 100, 148, 197, 355, 365
SfaNI GCATC 2 cut(s) 112, 469
SinI GGWCC 1 cut(s) 40
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 2 cut(s) 56, 231
SmoI CTYRAG 2 cut(s) 56, 231
Sse9I AATT 1 cut(s) 108
SsiI CCGC 1 cut(s) 32
SspMI CTAG 1 cut(s) 339
StyD4I CCNGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 392, 462
TaqI TCGA 1 cut(s) 404
TaqII GACCGA 1 cut(s) 358
TasI AATT 1 cut(s) 108
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 6 cut(s) 19, 346, 401, 491, 503, 509
TspDTI ATGAA 3 cut(s) 17, 396, 501
TspGWI ACGGA 1 cut(s) 281
VpaK11BI GGWCC 1 cut(s) 40
XceI RCATGY 1 cut(s) 178
XspI CTAG 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.