FvH4_4g04970

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013252
Physical Location & Seq
Reverse (-)
4290491 .. 4292610
2120 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04970.t1

Sequence Viewer

Length: 591 bp
ATGAAAGGAGGAAGGAAGAATCTGAAGAGGGCCGTGGAGGAGCAGAGCTTGACACTCAGCGATGGCCATTCTATAATGCAAGTGCTTTCTCTACGTGGTTCCAATCTCATTGAGGTAATGGATGCAAAAGGAGAGAAATATTTGGCGTTGTTTCCGGCTAAGTTTTCGAAGAGCATGTGGATAAAACAAGTGCCGATGTACCCAGAAAGGCAATGTTTTATGTTACTTTTTTTTTTTCATTGGGCGGGGAGCTTTGTTGTGGTTGATGCAAGTGAGAAGGACAAGGTTATTGAATCAGGCGGTAAAGTGGCTTGTATTGTTTCTCAAGTTCTGTTTTTCGAACAAGTTCGTGCACTTCGGAAATCACCAGAATGGCCTGAAATCTTCAAATCCACGGTCTCAGAAGAACCATGTGGAAGTCTGAAGGGACACACCTCCAAGCAAGAAGGGAATGCGAATGACAATGAGGATGACTCTAGTGATGATGATGGACTTCCTCCATTAGAAGCCAACATGAACAGGATGAGACCGGCTGAATGGCAATCAGAGGAAGAATCGGATTCAGATACAGATTCAGGAACTGATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
eIF-1a

Protein Analysis

197

Amino Acids

21.96

Weight (kDa)

4.86

Isoelectric Point (pI)

51.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
eIF-1a PF01176 23 - 63 1.3e-06 Translation initiation factor 1A / IF-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013252)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G40780 AT2G40780
fragaria_vesca FvH4_4g04970 FvH4_7g26120
malus_domestica MD01G1169400.v1.1
prunus_persica Prupe.2G265600_v2.0.a1
pyrus_communis pycom01g18210 pycom07g21280
rosa_chinensis RchiOBHm_Chr1g0372561
rosa_laevigata RLG00000026891
rosa_multiflora Rmu_co8142570.1_g000001 Rmu_sc0011709.1_g000001
rosa_roxburghii Rroxscaffold_4G00284850
rosa_rugosa Rorug01G0372800
rosa_samantha Rh1AG381300 Rh1BG344700 Rh1CG358100
rosa_wichuraiana Rw1G033530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 245, 300
AcoI YGGCCR 1 cut(s) 64
AcuI CTGAAG 2 cut(s) 44, 443
AfaI GTAC 1 cut(s) 200
AgsI TTSAA 2 cut(s) 293, 388
AluBI AGCT 2 cut(s) 48, 252
AluI AGCT 2 cut(s) 48, 252
Alw21I GWGCWC 1 cut(s) 355
Alw26I GTCTC 2 cut(s) 403, 520
Alw44I GTGCAC 1 cut(s) 351
AlwNI CAGNNNCTG 1 cut(s) 581
AoxI GGCC 3 cut(s) 30, 64, 374
ApaLI GTGCAC 1 cut(s) 351
ArsI GACNNNNNNTTYG 2 cut(s) 381, 413
Asp700I GAANNNNTTC 2 cut(s) 345, 583
AspS9I GGNCC 1 cut(s) 30
AsuHPI GGTGA 1 cut(s) 357
AsuII TTCGAA 2 cut(s) 167, 339
BaeGI GKGCMC 1 cut(s) 355
BalI TGGCCA 1 cut(s) 66
Bbv12I GWGCWC 1 cut(s) 355
BccI CCATC 2 cut(s) 56, 482
BceAI ACGGC 1 cut(s) 17
BcoDI GTCTC 2 cut(s) 403, 520
BfaI CTAG 1 cut(s) 477
BmgT120I GGNCC 1 cut(s) 30
BmiI GGNNCC 1 cut(s) 100
BmsI GCATC 2 cut(s) 112, 256
BplI GAGNNNNNCTC 2 cut(s) 458, 490
Bpu14I TTCGAA 2 cut(s) 167, 339
BpuEI CTTGAG 1 cut(s) 309
BsaAI YACGTR 1 cut(s) 95
BsaI GGTCTC 2 cut(s) 403, 520
BsaJI CCNNGG 2 cut(s) 33, 393
Bse118I RCCGGY 1 cut(s) 529
Bse3DI GCAATG 1 cut(s) 218
BseDI CCNNGG 2 cut(s) 33, 393
BseGI GGATG 3 cut(s) 127, 475, 528
BseMI GCAATG 1 cut(s) 218
BseMII CTCAG 2 cut(s) 70, 414
BseRI GAGGAG 1 cut(s) 53
BseSI GKGCMC 1 cut(s) 355
BshFI GGCC 3 cut(s) 32, 66, 376
BsiHKAI GWGCWC 1 cut(s) 355
BsiSI CCGG 2 cut(s) 155, 530
BslFI GGGAC 1 cut(s) 441
BsmAI GTCTC 2 cut(s) 403, 520
BsmFI GGGAC 1 cut(s) 441
BsmI GAATGC 1 cut(s) 457
BsnI GGCC 3 cut(s) 32, 66, 376
Bso31I GGTCTC 2 cut(s) 403, 520
Bsp119I TTCGAA 2 cut(s) 167, 339
Bsp1286I GDGCHC 1 cut(s) 355
BspACI CCGC 2 cut(s) 245, 300
BspANI GGCC 3 cut(s) 32, 66, 376
BspCNI CTCAG 2 cut(s) 69, 413
BspLI GGNNCC 1 cut(s) 100
BspQI GCTCTTC 1 cut(s) 164
BspT104I TTCGAA 2 cut(s) 167, 339
BspTNI GGTCTC 2 cut(s) 403, 520
BsrDI GCAATG 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 529
BssAI RCCGGY 1 cut(s) 529
BssECI CCNNGG 2 cut(s) 33, 393
Bst4CI ACNGT 1 cut(s) 397
Bst6I CTCTTC 2 cut(s) 20, 164
BstBAI YACGTR 1 cut(s) 95
BstBI TTCGAA 2 cut(s) 167, 339
BstDEI CTNAG 3 cut(s) 56, 159, 400
BstDSI CCRYGG 2 cut(s) 33, 393
BstF5I GGATG 3 cut(s) 127, 475, 528
BstMAI GTCTC 2 cut(s) 403, 520
BstNSI RCATGY 1 cut(s) 178
BstSLI GKGCMC 1 cut(s) 355
BsuRI GGCC 3 cut(s) 32, 66, 376
BtgI CCRYGG 2 cut(s) 33, 393
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 3 cut(s) 127, 475, 528
CaiI CAGNNNCTG 1 cut(s) 581
Cfr10I RCCGGY 1 cut(s) 529
Cfr13I GGNCC 1 cut(s) 30
Csp6I GTAC 1 cut(s) 199
CviAII CATG 3 cut(s) 175, 411, 514
CviJI RGCY 9 cut(s) 32, 48, 66, 158, 252, 311, 376, 509, 533
CviKI_1 RGCY 9 cut(s) 32, 48, 66, 158, 252, 311, 376, 509, 533
CviQI GTAC 1 cut(s) 199
DdeI CTNAG 3 cut(s) 56, 159, 400
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 20, 164
EarI CTCTTC 2 cut(s) 20, 164
Eco31I GGTCTC 2 cut(s) 403, 520
Eco57I CTGAAG 2 cut(s) 44, 443
FaeI CATG 3 cut(s) 178, 414, 517
FaiI YATR 5 cut(s) 74, 176, 221, 412, 515
FaqI GGGAC 1 cut(s) 441
FatI CATG 3 cut(s) 174, 410, 513
FauI CCCGC 1 cut(s) 238
FokI GGATG 3 cut(s) 134, 482, 535
FspBI CTAG 1 cut(s) 477
HaeIII GGCC 3 cut(s) 32, 66, 376
HapII CCGG 2 cut(s) 155, 530
Hin1II CATG 3 cut(s) 178, 414, 517
HinfI GANTC 7 cut(s) 19, 293, 473, 554, 560, 572, 584
HpaII CCGG 2 cut(s) 155, 530
HphI GGTGA 1 cut(s) 357
Hpy166II GTNNAC 1 cut(s) 353
Hpy188I TCNGA 7 cut(s) 24, 360, 403, 423, 547, 559, 565
Hpy188III TCNNGA 2 cut(s) 576, 588
Hpy8I GTNNAC 1 cut(s) 353
HpyAV CCTTC 4 cut(s) 6, 271, 418, 440
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 1 cut(s) 94
HpyCH4V TGCA 4 cut(s) 79, 125, 269, 353
HpyF3I CTNAG 3 cut(s) 56, 159, 400
HpySE526I ACGT 1 cut(s) 94
Hsp92II CATG 3 cut(s) 178, 414, 517
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 2 cut(s) 40, 249
LpnPI CCDG 8 cut(s) 168, 216, 282, 381, 390, 505, 543, 561
LweI GCATC 2 cut(s) 112, 256
MaeI CTAG 1 cut(s) 477
MaeII ACGT 1 cut(s) 94
MaeIII GTNAC 1 cut(s) 222
MboII GAAGA 6 cut(s) 28, 37, 181, 376, 416, 563
MhlI GDGCHC 1 cut(s) 355
MlsI TGGCCA 1 cut(s) 66
MluNI TGGCCA 1 cut(s) 66
MlyI GAGTC 1 cut(s) 467
MnlI CCTC 7 cut(s) 21, 31, 106, 445, 460, 507, 541
Mox20I TGGCCA 1 cut(s) 66
MroXI GAANNNNTTC 2 cut(s) 345, 583
MscI TGGCCA 1 cut(s) 66
MslI CAYNNNNRTG 1 cut(s) 370
Msp20I TGGCCA 1 cut(s) 66
MspI CCGG 2 cut(s) 155, 530
Mva1269I GAATGC 1 cut(s) 457
NlaIII CATG 3 cut(s) 178, 414, 517
NlaIV GGNNCC 1 cut(s) 100
NspI RCATGY 1 cut(s) 178
NspV TTCGAA 2 cut(s) 167, 339
PciSI GCTCTTC 1 cut(s) 164
PctI GAATGC 1 cut(s) 457
PdmI GAANNNNTTC 2 cut(s) 345, 583
PfeI GAWTC 6 cut(s) 19, 293, 554, 560, 572, 584
PleI GAGTC 1 cut(s) 467
PpsI GAGTC 1 cut(s) 467
Ppu21I YACGTR 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 100
PspPI GGNCC 1 cut(s) 30
PstNI CAGNNNCTG 1 cut(s) 581
RsaI GTAC 1 cut(s) 200
RsaNI GTAC 1 cut(s) 199
RseI CAYNNNNRTG 1 cut(s) 370
SapI GCTCTTC 1 cut(s) 164
Sau96I GGNCC 1 cut(s) 30
SchI GAGTC 1 cut(s) 467
SduI GDGCHC 1 cut(s) 355
SetI ASST 6 cut(s) 50, 97, 117, 254, 288, 437
SfaNI GCATC 2 cut(s) 112, 256
SfuI TTCGAA 2 cut(s) 167, 339
SmiMI CAYNNNNRTG 1 cut(s) 370
SmlI CTYRAG 1 cut(s) 324
SmoI CTYRAG 1 cut(s) 324
SsiI CCGC 2 cut(s) 245, 300
SspI AATATT 1 cut(s) 140
SspMI CTAG 1 cut(s) 477
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 1 cut(s) 97
TaqI TCGA 2 cut(s) 167, 339
TfiI GAWTC 6 cut(s) 19, 293, 554, 560, 572, 584
TspDTI ATGAA 3 cut(s) 17, 227, 530
VneI GTGCAC 1 cut(s) 351
XceI RCATGY 1 cut(s) 178
XmnI GAANNNNTTC 2 cut(s) 345, 583
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.