AT2G44160

Methylenetetrahydrofolate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0011051
Physical Location & Seq
Forward (+)
18262189 .. 18265346
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44160.1

Sequence Viewer

Length: 1785 bp
ATGAAGGTGATAGATAAGATCCAGTCATTAGCCGACGAAGGCAAAACAGCGTTTTCCTTCGAATTCTTCCCACCAAAGACAGAAGATGGAGTCGACAATCTCTTTGAGAGAATGGATCGAATGGTTGCTTATGGTCCAACGTTTTGCGATATCACTTGGGGAGCTGGTGGATCTACCGCAGATCTAACGCTTGATATAGCTTCGAGGATGCAAAACGTTGTTTGTGTTGAGAGTATGATGCATCTTACGTGTACTAACATGCCTGTTGAGAAGATTGATCATGCTCTTGAGACTATTAGATCTAATGGGATTCAGAATGTGCTTGCTCTTAGAGGTGATCCTCCTCATGGTCAAGATAAGTTTGTTCAGGTTGAAGGTGGTTTTGATTGTGCTTTGGATCTTGTTAATCACATTCGTAGCAAGTATGGTGATTACTTTGGTATCACTGTCGCTGGTTATCCTGAGGCGCATCCTGATGTTATTGGTGAGAATGGTCTTGCGTCTAATGAAGCTTATCAGAGTGATCTTGAGTATCTGAAGAAAAAGATTGATGCTGGAGCTGATCTGATTGTTACTCAGCTTTTCTATGATACTGATATATTCCTCAAGTTTGTGAATGATTGTCGGCAAATTGGGATTAGTTGTCCCATTGTTCCTGGAATTATGCCTATTAATAACTACAGAGGCTTTTTGCGTATGACTGGTTTCTGCAAGACTAAGATACCAGTTGAGGTGATGGCTGCCTTGGAGCCTATCAAGGATAACGAAGAAGCTGTGAAAGCCTATGGTATTCACCTTGGAACAGAGATGTGTAAAAAGATGTTGGCTCATGGAGTCAAGTCTCTTCATCTCTACACATTGAACATGGAGAAATCTGCTCTTGCAATATTGATGAATCTTGGTATGATTGATGAGTCCAAAATTTCTCGTTCTTTACCCTGGAGACGCCCTGCAAATGTTTTCCGTACTAAGGAAGATGTGCGCCCCATTTTCTGGGCAAACCGTCCAAAGAGCTACATTTCTAGAACCAAGGGCTGGGAAGATTTCCCACAAGGCCGGTGGGGTGATTCACGCAGTGCTTCATATGGTGCACTCTCGGATCATCAGTTCTCACGTCCGCGAGCACGTGACAAGAAGCTTCAACAAGAATGGGTTGTCCCACTGAAAAGTGTTGAAGATATTCAGGAGAAATTCAAGGAGCTCTGCCTTGGAAACCTTAAAAGCAGTCCCTGGTCTGAATTAGATGGACTCCAGCCAGAGACAAGGATAATAAACGAGCAACTGATAAAAGTCAACTCCAAAGGCTTCTTGACCATCAATAGCCAACCATCAGTCAACGCCGAGAGATCTGATTCCCCAACTGTTGGATGGGGAGGTCCTGTTGGATATGTATACCAAAAGGCTTATCTAGAGTTCTTCTGCTCAAAGGAGAAATTAGATGCAGTGGTGGAGAAATGCAAAGCTTTGCCATCGATTACTTACATGGCTGTGAACAAAGGAGAACAGTGGGTATCGAACACTGCCCAAGCTGATGTGAATGCTGTAACTTGGGGAGTTTTCCCGGCTAAGGAAATCATTCAACCAACCATTGTCGATCCCGCTAGCTTCAACGTCTGGAAGGATGAAGCATTTGAGACTTGGTCAAGAAGCTGGGCTAACTTGTACCCAGAAGCCGACCCTTCCAGAAACTTGCTCGAGGAGGTGAAGAACAGCTACTATTTGGTAAGCTTGGTGGAGAACGATTACATCAATGGTGACATATTCGCGGTGTTTGCTGATCTTTGA

Protein Analysis

594

Amino Acids

66.8

Weight (kDa)

5.33

Isoelectric Point (pI)

36.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTHFR PF02219 6 - 301 9.2e-126 Methylenetetrahydrofolate reductase
MTHFR_C PF21895 310 - 584 1.1e-87 MTHFR, SAM-binding regulatory domain
DUF6919 PF21897 403 - 487 1e-05 Domain of unknown function (DUF6919)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44160 AT3G59970 AT3G59970
fragaria_vesca FvH4_1g00040 FvH4_1g00040
malus_domestica MD00G1145100.v1.1 MD02G1001200.v1.1 MD15G1141700.v1.1
prunus_persica Prupe.7G271500_v2.0.a1
pyrus_communis pycom15g12800
rosa_chinensis RchiOBHm_Chr2g0084261
rosa_laevigata RLG00000015604
rosa_multiflora Rmu_sc0003129.1_g000016
rosa_roxburghii Rroxscaffold_2G00156200
rosa_rugosa Rorug01G0452200
rosa_samantha Rh2AG000600 Rh2BG000500 Rh2CG000400 Rh2DG000600
rosa_wichuraiana Rw2G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 993, 1035, 1364
AccI GTMKAC 2 cut(s) 93, 1392
AccII CGCG 2 cut(s) 1120, 1766
AciI CCGC 4 cut(s) 177, 1118, 1599, 1766
AclI AACGTT 2 cut(s) 140, 216
AclWI GGATC 7 cut(s) 13, 123, 178, 332, 405, 1107, 1589
AcsI RAATTY 3 cut(s) 62, 921, 1190
AcuI CTGAAG 1 cut(s) 557
AcvI CACGTG 1 cut(s) 1127
AcyI GRCGYC 1 cut(s) 946
AdeI CACNNNGTG 1 cut(s) 1076
AfaI GTAC 3 cut(s) 253, 967, 1664
AfiI CCNNNNNNNGG 6 cut(s) 347, 970, 993, 1035, 1364, 1567
AflIII ACRYGT 1 cut(s) 248
AgsI TTSAA 7 cut(s) 374, 862, 1142, 1175, 1195, 1580, 1609
AjiI CACGTC 1 cut(s) 1115
AjnI CCWGG 3 cut(s) 655, 938, 1229
AloI GAACNNNNNNTCC 2 cut(s) 1091, 1123
Alw21I GWGCWC 3 cut(s) 1093, 1126, 1203
Alw26I GTCTC 5 cut(s) 284, 846, 937, 1253, 1628
Alw44I GTGCAC 1 cut(s) 1089
AlwI GGATC 7 cut(s) 13, 123, 178, 332, 405, 1107, 1589
AlwNI CAGNNNCTG 1 cut(s) 1230
Ama87I CYCGRG 1 cut(s) 1694
AoxI GGCC 1 cut(s) 1054
ApaLI GTGCAC 1 cut(s) 1089
ApeKI GCWGC 1 cut(s) 740
ApoI RAATTY 3 cut(s) 62, 921, 1190
ArsI GACNNNNNNTTYG 2 cut(s) 86, 118
AseI ATTAAT 1 cut(s) 672
Asp700I GAANNNNTTC 2 cut(s) 1179, 1575
AspLEI GCGC 2 cut(s) 469, 984
AspS9I GGNCC 2 cut(s) 134, 1376
AsuC2I CCSGG 1 cut(s) 1562
AsuHPI GGTGA 9 cut(s) 19, 347, 440, 497, 745, 785, 1076, 1714, 1766
AsuII TTCGAA 1 cut(s) 60
AsuNHI GCTAGC 1 cut(s) 1601
AvaI CYCGRG 1 cut(s) 1694
AvaII GGWCC 2 cut(s) 134, 1376
AxyI CCTNAGG 1 cut(s) 462
BaeGI GKGCMC 1 cut(s) 1093
BaeI ACNNNNGTAYC 2 cut(s) 424, 457
BanII GRGCYC 1 cut(s) 1203
BarI GAAGNNNNNNTAC 2 cut(s) 1697, 1729
BbrPI CACGTG 1 cut(s) 1127
Bbv12I GWGCWC 3 cut(s) 1093, 1126, 1203
BbvI GCAGC 1 cut(s) 727
BccI CCATC 7 cut(s) 80, 730, 1238, 1322, 1336, 1362, 1477
BciT130I CCWGG 3 cut(s) 657, 940, 1231
BclI TGATCA 1 cut(s) 277
BcnI CCSGG 1 cut(s) 1562
BcoDI GTCTC 5 cut(s) 284, 846, 937, 1253, 1628
BfaI CTAG 3 cut(s) 1023, 1409, 1602
BfmI CTRYAG 1 cut(s) 679
BglII AGATCT 3 cut(s) 181, 299, 1346
BisI GCNGC 1 cut(s) 741
BlsI GCNGC 1 cut(s) 742
Bme1390I CCNGG 4 cut(s) 657, 940, 1231, 1562
Bme18I GGWCC 2 cut(s) 134, 1376
BmeT110I CYCGRG 1 cut(s) 1694
BmgBI CACGTC 1 cut(s) 1115
BmgT120I GGNCC 2 cut(s) 134, 1376
BmiI GGNNCC 1 cut(s) 750
BmrFI CCNGG 4 cut(s) 657, 940, 1231, 1562
BmsI GCATC 6 cut(s) 198, 228, 250, 478, 541, 1429
BmtI GCTAGC 1 cut(s) 1605
BpmI CTGGAG 3 cut(s) 576, 961, 1235
Bpu10I CCTNAGC 1 cut(s) 1566
Bpu14I TTCGAA 1 cut(s) 60
BpuEI CTTGAG 3 cut(s) 308, 548, 590
BpuMI CCSGG 1 cut(s) 1562
Bsa29I ATCGAT 1 cut(s) 1472
BsaAI YACGTR 2 cut(s) 249, 1127
BsaHI GRCGYC 1 cut(s) 946
BsaJI CCNNGG 6 cut(s) 744, 796, 938, 1029, 1207, 1229
BsaXI ACNNNNNCTCC 2 cut(s) 1365, 1395
Bsc4I CCNNNNNNNGG 6 cut(s) 347, 970, 993, 1035, 1364, 1567
Bse118I RCCGGY 1 cut(s) 1056
Bse1I ACTGG 3 cut(s) 22, 706, 725
Bse21I CCTNAGG 1 cut(s) 462
BseBI CCWGG 3 cut(s) 657, 940, 1231
BseCI ATCGAT 1 cut(s) 1472
BseDI CCNNGG 6 cut(s) 744, 796, 938, 1029, 1207, 1229
BseGI GGATG 4 cut(s) 213, 469, 1373, 1627
BseLI CCNNNNNNNGG 6 cut(s) 347, 970, 993, 1035, 1364, 1567
BseMII CTCAG 2 cut(s) 453, 590
BseNI ACTGG 3 cut(s) 22, 706, 725
BseRI GAGGAG 2 cut(s) 333, 1712
BseSI GKGCMC 1 cut(s) 1093
BseXI GCAGC 1 cut(s) 727
BseYI CCCAGC 2 cut(s) 1035, 1650
Bsh1236I CGCG 2 cut(s) 1120, 1766
BshFI GGCC 1 cut(s) 1056
BshVI ATCGAT 1 cut(s) 1472
BsiHKAI GWGCWC 3 cut(s) 1093, 1126, 1203
BsiHKCI CYCGRG 1 cut(s) 1694
BsiSI CCGG 2 cut(s) 1057, 1562
BslFI GGGAC 3 cut(s) 630, 1142, 1212
BslI CCNNNNNNNGG 6 cut(s) 347, 970, 993, 1035, 1364, 1567
BsmAI GTCTC 5 cut(s) 284, 846, 937, 1253, 1628
BsmBI CGTCTC 1 cut(s) 937
BsmFI GGGAC 3 cut(s) 630, 1142, 1212
BsmI GAATGC 1 cut(s) 1543
BsnI GGCC 1 cut(s) 1056
BsoBI CYCGRG 1 cut(s) 1694
Bsp119I TTCGAA 1 cut(s) 60
Bsp1286I GDGCHC 3 cut(s) 1093, 1126, 1203
BspACI CCGC 4 cut(s) 177, 1118, 1599, 1766
BspANI GGCC 1 cut(s) 1056
BspCNI CTCAG 2 cut(s) 454, 589
BspDI ATCGAT 1 cut(s) 1472
BspFNI CGCG 2 cut(s) 1120, 1766
BspLI GGNNCC 1 cut(s) 750
BspOI GCTAGC 1 cut(s) 1605
BspPI GGATC 7 cut(s) 13, 123, 178, 332, 405, 1107, 1589
BspT104I TTCGAA 1 cut(s) 60
BsrFI RCCGGY 1 cut(s) 1056
BsrI ACTGG 3 cut(s) 22, 706, 725
BssAI RCCGGY 1 cut(s) 1056
BssECI CCNNGG 6 cut(s) 744, 796, 938, 1029, 1207, 1229
BssNAI GTATAC 1 cut(s) 1393
BssNI GRCGYC 1 cut(s) 946
BssT1I CCWWGG 4 cut(s) 744, 796, 1029, 1207
Bst1107I GTATAC 1 cut(s) 1393
Bst2UI CCWGG 3 cut(s) 657, 940, 1231
Bst4CI ACNGT 4 cut(s) 448, 1004, 1363, 1506
Bst6I CTCTTC 1 cut(s) 849
BstACI GRCGYC 1 cut(s) 946
BstBAI YACGTR 2 cut(s) 249, 1127
BstBI TTCGAA 1 cut(s) 60
BstC8I GCNNGC 3 cut(s) 324, 1122, 1603
BstDEI CTNAG 6 cut(s) 329, 462, 576, 717, 969, 1566
BstF5I GGATG 4 cut(s) 213, 469, 1373, 1627
BstFNI CGCG 2 cut(s) 1120, 1766
BstHHI GCGC 2 cut(s) 469, 984
BstMAI GTCTC 5 cut(s) 284, 846, 937, 1253, 1628
BstMWI GCNNNNNNNGC 2 cut(s) 779, 1772
BstNI CCWGG 3 cut(s) 657, 940, 1231
BstNSI RCATGY 1 cut(s) 262
BstSCI CCNGG 4 cut(s) 655, 938, 1229, 1560
BstSFI CTRYAG 1 cut(s) 679
BstSLI GKGCMC 1 cut(s) 1093
BstUI CGCG 2 cut(s) 1120, 1766
BstV1I GCAGC 1 cut(s) 727
BstX2I RGATCY 6 cut(s) 18, 170, 181, 299, 397, 1346
BstYI RGATCY 6 cut(s) 18, 170, 181, 299, 397, 1346
BstZ17I GTATAC 1 cut(s) 1393
Bsu15I ATCGAT 1 cut(s) 1472
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 1 cut(s) 1056
BsuTUI ATCGAT 1 cut(s) 1472
BtrI CACGTC 1 cut(s) 1115
BtsCI GGATG 4 cut(s) 213, 469, 1373, 1627
BtsI GCAGTG 3 cut(s) 1081, 1449, 1518
BtsIMutI CAGTG 6 cut(s) 444, 1081, 1160, 1449, 1511, 1518
Cac8I GCNNGC 3 cut(s) 324, 1122, 1603
CaiI CAGNNNCTG 1 cut(s) 1230
CfoI GCGC 2 cut(s) 469, 984
Cfr10I RCCGGY 1 cut(s) 1056
Cfr13I GGNCC 2 cut(s) 134, 1376
ClaI ATCGAT 1 cut(s) 1472
CseI GACGC 2 cut(s) 489, 954
Csp6I GTAC 3 cut(s) 252, 966, 1663
CspCI CAANNNNNGTGG 2 cut(s) 1040, 1075
CviAII CATG 6 cut(s) 259, 281, 347, 830, 865, 1483
CviQI GTAC 3 cut(s) 252, 966, 1663
DdeI CTNAG 6 cut(s) 329, 462, 576, 717, 969, 1566
DraIII CACNNNGTG 1 cut(s) 1076
Eam1104I CTCTTC 1 cut(s) 849
EarI CTCTTC 1 cut(s) 849
Ecl136II GAGCTC 1 cut(s) 1201
Eco130I CCWWGG 4 cut(s) 744, 796, 1029, 1207
Eco24I GRGCYC 1 cut(s) 1203
Eco32I GATATC 1 cut(s) 151
Eco47I GGWCC 2 cut(s) 134, 1376
Eco53kI GAGCTC 1 cut(s) 1201
Eco57I CTGAAG 1 cut(s) 557
Eco72I CACGTG 1 cut(s) 1127
Eco81I CCTNAGG 1 cut(s) 462
Eco88I CYCGRG 1 cut(s) 1694
EcoICRI GAGCTC 1 cut(s) 1201
EcoO109I RGGNCCY 1 cut(s) 1376
EcoRI GAATTC 1 cut(s) 62
EcoRII CCWGG 3 cut(s) 655, 938, 1229
EcoRV GATATC 1 cut(s) 151
EcoT14I CCWWGG 4 cut(s) 744, 796, 1029, 1207
EcoT22I ATGCAT 1 cut(s) 243
EcoT38I GRGCYC 1 cut(s) 1203
ErhI CCWWGG 4 cut(s) 744, 796, 1029, 1207
Esp3I CGTCTC 1 cut(s) 937
FaeI CATG 6 cut(s) 262, 284, 350, 833, 868, 1486
FaqI GGGAC 3 cut(s) 630, 1142, 1212
FatI CATG 6 cut(s) 258, 280, 346, 829, 864, 1482
FauI CCCGC 1 cut(s) 1606
FauNDI CATATG 1 cut(s) 1084
FbaI TGATCA 1 cut(s) 277
FblI GTMKAC 2 cut(s) 93, 1392
Fnu4HI GCNGC 1 cut(s) 741
FokI GGATG 4 cut(s) 220, 456, 1380, 1634
FriOI GRGCYC 1 cut(s) 1203
Fsp4HI GCNGC 1 cut(s) 741
FspBI CTAG 3 cut(s) 1023, 1409, 1602
GlaI GCGC 2 cut(s) 468, 983
GluI GCNGC 1 cut(s) 741
GsaI CCCAGC 2 cut(s) 1039, 1654
GsuI CTGGAG 3 cut(s) 576, 961, 1235
HaeIII GGCC 1 cut(s) 1056
HapII CCGG 2 cut(s) 1057, 1562
HgaI GACGC 2 cut(s) 489, 954
HhaI GCGC 2 cut(s) 469, 984
Hin1I GRCGYC 1 cut(s) 946
Hin1II CATG 6 cut(s) 262, 284, 350, 833, 868, 1486
Hin6I GCGC 2 cut(s) 467, 982
HinP1I GCGC 2 cut(s) 467, 982
HincII GTYRAC 3 cut(s) 94, 1294, 1336
HindII GTYRAC 3 cut(s) 94, 1294, 1336
HindIII AAGCTT 4 cut(s) 510, 1136, 1461, 1726
HinfI GANTC 8 cut(s) 90, 310, 834, 895, 914, 1067, 1248, 1352
HpaII CCGG 2 cut(s) 1057, 1562
HphI GGTGA 9 cut(s) 19, 347, 440, 497, 745, 785, 1076, 1714, 1766
Hpy166II GTNNAC 7 cut(s) 94, 252, 1091, 1294, 1336, 1393, 1492
Hpy188I TCNGA 7 cut(s) 315, 519, 537, 567, 1099, 1237, 1351
Hpy8I GTNNAC 7 cut(s) 94, 252, 1091, 1294, 1336, 1393, 1492
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 5 cut(s) 32, 67, 368, 1612, 1689
HpyCH4III ACNGT 4 cut(s) 448, 1004, 1363, 1506
HpyCH4IV ACGT 6 cut(s) 140, 216, 248, 1114, 1126, 1611
HpyCH4V TGCA 8 cut(s) 211, 241, 711, 884, 953, 1091, 1442, 1458
HpyF10VI GCNNNNNNNGC 2 cut(s) 779, 1772
HpyF3I CTNAG 6 cut(s) 329, 462, 576, 717, 969, 1566
HpySE526I ACGT 6 cut(s) 140, 216, 248, 1114, 1126, 1611
Hsp92I GRCGYC 1 cut(s) 946
Hsp92II CATG 6 cut(s) 262, 284, 350, 833, 868, 1486
HspAI GCGC 2 cut(s) 467, 982
Ksp22I TGATCA 1 cut(s) 277
LmnI GCTCC 4 cut(s) 161, 557, 748, 1198
Lsp1109I GCAGC 1 cut(s) 727
LweI GCATC 6 cut(s) 198, 228, 250, 478, 541, 1429
MaeI CTAG 3 cut(s) 1023, 1409, 1602
MaeII ACGT 6 cut(s) 140, 216, 248, 1114, 1126, 1611
MaeIII GTNAC 4 cut(s) 571, 1127, 1543, 1754
MflI RGATCY 6 cut(s) 18, 170, 181, 299, 397, 1346
MhlI GDGCHC 3 cut(s) 1093, 1126, 1203
MluCI AATT 7 cut(s) 62, 630, 660, 921, 1190, 1238, 1433
MlyI GAGTC 4 cut(s) 99, 843, 923, 1242
MmeI TCCRAC 3 cut(s) 161, 1345, 1363
Mph1103I ATGCAT 1 cut(s) 243
MroXI GAANNNNTTC 2 cut(s) 1179, 1575
MseI TTAA 3 cut(s) 405, 672, 1218
MslI CAYNNNNRTG 2 cut(s) 474, 1487
MspI CCGG 2 cut(s) 1057, 1562
MspR9I CCNGG 4 cut(s) 657, 940, 1231, 1562
Mva1269I GAATGC 1 cut(s) 1543
MvaI CCWGG 3 cut(s) 657, 940, 1231
MvnI CGCG 2 cut(s) 1120, 1766
MwoI GCNNNNNNNGC 2 cut(s) 779, 1772
NciI CCSGG 1 cut(s) 1562
NdeI CATATG 1 cut(s) 1084
NheI GCTAGC 1 cut(s) 1601
NlaIII CATG 6 cut(s) 262, 284, 350, 833, 868, 1486
NlaIV GGNNCC 1 cut(s) 750
NmeAIII GCCGAG 1 cut(s) 1366
NmuCI GTSAC 2 cut(s) 1127, 1754
NsiI ATGCAT 1 cut(s) 243
NspI RCATGY 1 cut(s) 262
NspV TTCGAA 1 cut(s) 60
PaeR7I CTCGAG 1 cut(s) 1694
PctI GAATGC 1 cut(s) 1543
PdmI GAANNNNTTC 2 cut(s) 1179, 1575
PfeI GAWTC 4 cut(s) 310, 895, 1067, 1352
PflFI GACNNNGTC 1 cut(s) 1639
PflMI CCANNNNNTGG 3 cut(s) 993, 1035, 1364
PfoI TCCNGGA 1 cut(s) 655
PkrI GCNGC 1 cut(s) 742
PleI GAGTC 4 cut(s) 98, 842, 922, 1242
PmaCI CACGTG 1 cut(s) 1127
PmlI CACGTG 1 cut(s) 1127
PpsI GAGTC 4 cut(s) 98, 842, 922, 1242
Ppu21I YACGTR 2 cut(s) 249, 1127
PpuMI RGGWCCY 1 cut(s) 1376
PshBI ATTAAT 1 cut(s) 672
Psp124BI GAGCTC 1 cut(s) 1203
Psp1406I AACGTT 2 cut(s) 140, 216
Psp5II RGGWCCY 1 cut(s) 1376
Psp6I CCWGG 3 cut(s) 655, 938, 1229
PspCI CACGTG 1 cut(s) 1127
PspFI CCCAGC 2 cut(s) 1035, 1650
PspGI CCWGG 3 cut(s) 655, 938, 1229
PspN4I GGNNCC 1 cut(s) 750
PspPI GGNCC 2 cut(s) 134, 1376
PspPPI RGGWCCY 1 cut(s) 1376
PspXI VCTCGAGB 1 cut(s) 1694
PstNI CAGNNNCTG 1 cut(s) 1230
PsuI RGATCY 6 cut(s) 18, 170, 181, 299, 397, 1346
PsyI GACNNNGTC 1 cut(s) 1639
RsaI GTAC 3 cut(s) 253, 967, 1664
RsaNI GTAC 3 cut(s) 252, 966, 1663
RseI CAYNNNNRTG 2 cut(s) 474, 1487
SacI GAGCTC 1 cut(s) 1203
SalI GTCGAC 1 cut(s) 92
SaqAI TTAA 3 cut(s) 405, 672, 1218
SatI GCNGC 1 cut(s) 741
Sau96I GGNCC 2 cut(s) 134, 1376
SchI GAGTC 4 cut(s) 99, 843, 923, 1242
ScrFI CCNGG 4 cut(s) 657, 940, 1231, 1562
SduI GDGCHC 3 cut(s) 1093, 1126, 1203
SfaNI GCATC 6 cut(s) 198, 228, 250, 478, 541, 1429
SfcI CTRYAG 1 cut(s) 679
Sfr274I CTCGAG 1 cut(s) 1694
SfuI TTCGAA 1 cut(s) 60
SinI GGWCC 2 cut(s) 134, 1376
SlaI CTCGAG 1 cut(s) 1694
SmiMI CAYNNNNRTG 2 cut(s) 474, 1487
SmlI CTYRAG 4 cut(s) 287, 527, 605, 1694
SmoI CTYRAG 4 cut(s) 287, 527, 605, 1694
Sse9I AATT 7 cut(s) 62, 630, 660, 921, 1190, 1238, 1433
SsiI CCGC 4 cut(s) 177, 1118, 1599, 1766
SspI AATATT 1 cut(s) 888
SspMI CTAG 3 cut(s) 1023, 1409, 1602
SstI GAGCTC 1 cut(s) 1203
StyD4I CCNGG 4 cut(s) 655, 938, 1229, 1560
StyI CCWWGG 4 cut(s) 744, 796, 1029, 1207
TaaI ACNGT 4 cut(s) 448, 1004, 1363, 1506
TaiI ACGT 6 cut(s) 143, 219, 251, 1117, 1129, 1614
TaqI TCGA 8 cut(s) 60, 93, 118, 203, 1472, 1514, 1593, 1695
TasI AATT 7 cut(s) 62, 630, 660, 921, 1190, 1238, 1433
TatI WGTACW 1 cut(s) 251
TfiI GAWTC 4 cut(s) 310, 895, 1067, 1352
Tru1I TTAA 3 cut(s) 405, 672, 1218
Tru9I TTAA 3 cut(s) 405, 672, 1218
TscAI CASTG 6 cut(s) 451, 1081, 1167, 1449, 1511, 1525
TseFI GTSAC 2 cut(s) 1127, 1754
TseI GCWGC 1 cut(s) 740
Tsp45I GTSAC 2 cut(s) 1127, 1754
TspDTI ATGAA 6 cut(s) 17, 522, 836, 908, 1071, 1638
TspGWI ACGGA 1 cut(s) 953
TspRI CASTG 6 cut(s) 451, 1081, 1167, 1449, 1511, 1525
Tth111I GACNNNGTC 1 cut(s) 1639
Van91I CCANNNNNTGG 3 cut(s) 993, 1035, 1364
VneI GTGCAC 1 cut(s) 1089
VpaK11BI GGWCC 2 cut(s) 134, 1376
VspI ATTAAT 1 cut(s) 672
XapI RAATTY 3 cut(s) 62, 921, 1190
XbaI TCTAGA 2 cut(s) 1022, 1408
XceI RCATGY 1 cut(s) 262
XcmI CCANNNNNNNNNTGG 2 cut(s) 1056, 1365
XhoI CTCGAG 1 cut(s) 1694
XmiI GTMKAC 2 cut(s) 93, 1392
XmnI GAANNNNTTC 2 cut(s) 1179, 1575
XspI CTAG 3 cut(s) 1023, 1409, 1602
Zsp2I ATGCAT 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.