Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0011051

Physical Location & Seq

Genomic Coordinates Reverse (-)

95592 .. 99815

Gene Structure 4224 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000015604

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGAAGGTGATCGACAAAATCCACGAGGCATTGGCGGATGAGAAGAAGGTGGTGTTCTCGTTCGAGTTCTTCCCTCCGAAGACGGAGGATGGGGTGGAGAACCTGCTGGAGAGAATGGATCGGATGGTGTCTCACAATCCGGCCTTCTGCGACATCACTTGGGGCGCCGGTGGCTCCACCGCCGATCTCACTCTGGACATCGCCAACAAAATGCAGAACATGATCTGCGTCGAGAGCATGATGCATCTCACCTGCACCAACATGCCCGTCGACAAGATCGACCACGCCCTCCAGACTATCAAGTCCAACGGCCTCCAGAATGTTCTTGCTCTCCGCGGCGACCCACCCCATGGCCAGGACAAGTTTGTTCAGATCCAAGGCGGCTTTGCCTGCGCCCTCGACCTGGTCACTCATATCAGAGCCACATATGGTGACTACTTTGGTATCACTGTTGCTGGTTATCCAGAGGCACATCCCGATGCCATTGAAGCCGATGGCCTCGCCACTCCAGAGTCCTATCAAAGTGATCTGGCTTATCTCAAGAGAAAGGTTGATGCCGGTGCTGATTTGATCATCACTCAATTATTCTACGATACTGATGTCTTTCTCAAATTCGTCAACGACTGTCGCCAAATTGGAATCACTTGTCCCATTGTTCCTGGAATTATGCCTATTAATAACTACAAAGGTTTCATACGCATGACTGGTTTCTGCAAAACAAGGATACCAGCTGAGGTTACTGCTGCCTTGGAACCTATTAAGGACAATGAAGAGGCTGTCAGAGCTTATGGAATTCACCTTGGAACAGAAATGTGCAAGAAGATTTTAGCTCATGGGATTAGGACATTGCATCTTTATACACTGAACATGGAGAAATCAGCATTGGCTATACTAATGAATCTTGGTTTGATTGAAGAGGCCAAAATATCAAGGCCCTTACCTTGGAGACGCCCTGCAAATGTTTTTCGTGTTAAAGAAGATGTTCGTCCAATTTTCTGGGCTAATCGTCCTAAGAGCTACATATCAAGAACCATAGGCTGGGACCTGTACCCACATGGGCGGTGGGGTGATTCTCGTAATCCTTCATATGGCGCACTAACTGATTACCAGTTCATGCGACCACGTGCACGTGACAAGAAACTTGTTGAAGAATGGGTTGTCCCACTGAAAAGCGTTGAAGATATTCATGAGAAATTTGAGAAATTCTGCCTTGGAAAATTGAGAAGTAGCCCTTGGTCAGAACTCGATGGGCTTCAGCCAGAGACAAAGATCATAAATGAGCAGCTGGGGAAAATTAACACAAAGGGTTTCCTTACCATCAACAGCCAACCAGCAATAAATGGGGAAAGATCTGATTCACCATCTGTTGGATGGGGTGGGCCTGGAGGATATGTTTACCAGAAAGCCTATGTGGAGTTTTTCTGTTCCAAGGAGAAGTTGGGTACTCTTGTTGACAAATGCAAGGCACTTCCATCTCTTACATACATGGCTGTTAATAGAGAAGGGAGTTGGATTTCTAATATCAGTCAAACTGATGTAAATGCGGTAACATGGGGAGTCTTCCCAGCAAAGGAGATAATCCAACCGACTGTCGTGGATCCTGCTAGTTTTATGGTTTGGAAGGATGAGGCATTTGAAATCTGGTCAAGAGGATGGGGTCGCTTGTACCCCGAGAGCGACTCATCTAGGAAATTACTTGAAGAGGTCCAGAGTAGCTACTTCTTGGTCAGCTTGGTAGATAATGACTACATCCATGGTGACCTTTTCAATGTTTTTGCTAATTTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004489 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 ko01523 map00670 map00720 map01100 map01120 map01200 map01523

Pfam Domains

Protein Families

MTHFR

Protein Analysis

596

Amino Acids

67.05

Weight (kDa)

5.88

Isoelectric Point (pI)

39.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTHFR	PF02219	9 - 302	6.4e-122	Methylenetetrahydrofolate reductase
MTHFR_C	PF21895	311 - 585	1.5e-91	MTHFR, SAM-binding regulatory domain
DUF6919	PF21897	391 - 488	1.7e-07	Domain of unknown function (DUF6919)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011051)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44160 AT3G59970 AT3G59970
fragaria_vesca	FvH4_1g00040 FvH4_1g00040
malus_domestica	MD00G1145100.v1.1 MD02G1001200.v1.1 MD15G1141700.v1.1
prunus_persica	Prupe.7G271500_v2.0.a1
pyrus_communis	pycom15g12800
rosa_chinensis	RchiOBHm_Chr2g0084261
rosa_laevigata	RLG00000015604
rosa_multiflora	Rmu_sc0003129.1_g000016
rosa_roxburghii	Rroxscaffold_2G00156200
rosa_rugosa	Rorug01G0452200
rosa_samantha	Rh2AG000600 Rh2BG000500 Rh2CG000400 Rh2DG000600
rosa_wichuraiana	Rw2G000040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	260
AasI	GACNNNNNNGTC	1 cut(s)	301
Acc36I	ACCTGC	2 cut(s)	111, 260
AccB1I	GGYRCC	1 cut(s)	164
AccB7I	CCANNNNNTGG	3 cut(s)	350, 1038, 1367
AccI	GTMKAC	1 cut(s)	270
AccII	CGCG	1 cut(s)	336
AciI	CCGC	7 cut(s)	35, 180, 334, 336, 381, 1060, 1544
AclWI	GGATC	4 cut(s)	126, 367, 1592, 1605
AcoI	YGGCCR	1 cut(s)	352
AcsI	RAATTY	4 cut(s)	611, 792, 1193, 1202
AcuI	CTGAAG	1 cut(s)	1238
AcvI	CACGTG	2 cut(s)	1124, 1130
AcyI	GRCGYC	2 cut(s)	165, 949
AfaI	GTAC	3 cut(s)	1049, 1444, 1667
AfiI	CCNNNNNNNGG	8 cut(s)	350, 355, 403, 942, 1038, 1088, 1367, 1570
AgsI	TTSAA	7 cut(s)	488, 914, 1148, 1178, 1637, 1700, 1768
AjnI	CCWGG	4 cut(s)	354, 402, 658, 1381
AjuI	GAANNNNNNNTTGG	4 cut(s)	866, 898, 1216, 1248
AluBI	AGCT	7 cut(s)	731, 785, 830, 1017, 1285, 1716, 1731
AluI	AGCT	7 cut(s)	731, 785, 830, 1017, 1285, 1716, 1731
Alw21I	GWGCWC	1 cut(s)	1129
Alw26I	GTCTC	3 cut(s)	135, 940, 1256
Alw44I	GTGCAC	1 cut(s)	1125
AlwI	GGATC	4 cut(s)	126, 367, 1592, 1605
Ama87I	CYCGRG	1 cut(s)	1670
AoxI	GGCC	7 cut(s)	141, 310, 352, 496, 918, 932, 1379
ApaLI	GTGCAC	1 cut(s)	1125
ApeKI	GCWGC	2 cut(s)	743, 1282
ApoI	RAATTY	4 cut(s)	611, 792, 1193, 1202
AseI	ATTAAT	1 cut(s)	675
Asp700I	GAANNNNTTC	2 cut(s)	981, 1182
AspLEI	GCGC	3 cut(s)	167, 395, 1094
AspS9I	GGNCC	4 cut(s)	933, 1042, 1379, 1705
AsuHPI	GGTGA	7 cut(s)	19, 241, 443, 788, 1079, 1350, 1769
AvaI	CYCGRG	1 cut(s)	1670
AvaII	GGWCC	2 cut(s)	1042, 1705
BaeGI	GKGCMC	1 cut(s)	1129
BaeI	ACNNNNGTAYC	6 cut(s)	427, 460, 585, 618, 1434, 1467
BalI	TGGCCA	1 cut(s)	354
BamHI	GGATCC	1 cut(s)	1597
BanI	GGYRCC	1 cut(s)	164
BauI	CACGAG	1 cut(s)	23
BbrPI	CACGTG	2 cut(s)	1124, 1130
BbsI	GAAGAC	2 cut(s)	86, 1552
Bbv12I	GWGCWC	1 cut(s)	1129
BbvCI	CCTCAGC	1 cut(s)	732
BbvI	GCAGC	2 cut(s)	730, 1294
BccI	CCATC	9 cut(s)	83, 118, 488, 1241, 1325, 1365, 1369, 1480, 1647
BceAI	ACGGC	1 cut(s)	325
BciT130I	CCWGG	4 cut(s)	356, 404, 660, 1383
BciVI	GTATCC	1 cut(s)	717
BclI	TGATCA	1 cut(s)	570
BcoDI	GTCTC	3 cut(s)	135, 940, 1256
BfaI	CTAG	3 cut(s)	1605, 1686, 1786
BfoI	RGCGCY	1 cut(s)	168
BfuAI	ACCTGC	2 cut(s)	111, 260
BfuI	GTATCC	1 cut(s)	717
BglII	AGATCT	1 cut(s)	1349
BisI	GCNGC	4 cut(s)	337, 382, 744, 1283
BlsI	GCNGC	4 cut(s)	338, 383, 745, 1284
Bme1390I	CCNGG	4 cut(s)	356, 404, 660, 1383
Bme18I	GGWCC	2 cut(s)	1042, 1705
BmeT110I	CYCGRG	1 cut(s)	1670
BmgT120I	GGNCC	4 cut(s)	933, 1042, 1379, 1705
BmiI	GGNNCC	5 cut(s)	166, 175, 753, 1043, 1599
BmrFI	CCNGG	4 cut(s)	356, 404, 660, 1383
BmsI	GCATC	5 cut(s)	231, 253, 469, 544, 859
BpiI	GAAGAC	2 cut(s)	86, 1552
BplI	GAGNNNNNCTC	2 cut(s)	1664, 1696
BpmI	CTGGAG	5 cut(s)	128, 275, 299, 492, 1404
Bpu10I	CCTNAGC	1 cut(s)	732
BpuEI	CTTGAG	1 cut(s)	524
BsaAI	YACGTR	2 cut(s)	1124, 1130
BsaHI	GRCGYC	2 cut(s)	165, 949
BsaXI	ACNNNNNCTCC	2 cut(s)	1377, 1407
Bsc4I	CCNNNNNNNGG	8 cut(s)	350, 355, 403, 942, 1038, 1088, 1367, 1570
Bse118I	RCCGGY	2 cut(s)	167, 557
Bse1I	ACTGG	2 cut(s)	709, 1108
Bse3DI	GCAATG	1 cut(s)	845
BseBI	CCWGG	4 cut(s)	356, 404, 660, 1383
BseGI	GGATG	8 cut(s)	43, 94, 129, 472, 1376, 1630, 1658, 1749
BseLI	CCNNNNNNNGG	8 cut(s)	350, 355, 403, 942, 1038, 1088, 1367, 1570
BseMI	GCAATG	1 cut(s)	845
BseMII	CTCAG	1 cut(s)	723
BseNI	ACTGG	2 cut(s)	709, 1108
BseSI	GKGCMC	1 cut(s)	1129
BseXI	GCAGC	2 cut(s)	730, 1294
BseYI	CCCAGC	3 cut(s)	1038, 1285, 1564
BsgI	GTGCAG	1 cut(s)	238
Bsh1236I	CGCG	1 cut(s)	336
BshFI	GGCC	7 cut(s)	143, 312, 354, 498, 920, 934, 1381
BshNI	GGYRCC	1 cut(s)	164
BsiHKAI	GWGCWC	1 cut(s)	1129
BsiHKCI	CYCGRG	1 cut(s)	1670
BsiSI	CCGG	3 cut(s)	140, 168, 558
BslFI	GGGAC	3 cut(s)	633, 1055, 1145
BslI	CCNNNNNNNGG	8 cut(s)	350, 355, 403, 942, 1038, 1088, 1367, 1570
BsmAI	GTCTC	3 cut(s)	135, 940, 1256
BsmBI	CGTCTC	1 cut(s)	940
BsmFI	GGGAC	3 cut(s)	633, 1055, 1145
BsnI	GGCC	7 cut(s)	143, 312, 354, 498, 920, 934, 1381
BsoBI	CYCGRG	1 cut(s)	1670
Bsp1286I	GDGCHC	1 cut(s)	1129
Bsp19I	CCATGG	2 cut(s)	349, 1753
BspACI	CCGC	7 cut(s)	35, 180, 334, 336, 381, 1060, 1544
BspANI	GGCC	7 cut(s)	143, 312, 354, 498, 920, 934, 1381
BspCNI	CTCAG	1 cut(s)	724
BspFNI	CGCG	1 cut(s)	336
BspHI	TCATGA	1 cut(s)	1186
BspLI	GGNNCC	5 cut(s)	166, 175, 753, 1043, 1599
BspMI	ACCTGC	2 cut(s)	111, 260
BspPI	GGATC	4 cut(s)	126, 367, 1592, 1605
BspT107I	GGYRCC	1 cut(s)	164
BsrDI	GCAATG	1 cut(s)	845
BsrFI	RCCGGY	2 cut(s)	167, 557
BsrI	ACTGG	2 cut(s)	709, 1108
BssAI	RCCGGY	2 cut(s)	167, 557
BssNI	GRCGYC	2 cut(s)	165, 949
BssSI	CACGAG	1 cut(s)	23
BssT1I	CCWWGG	9 cut(s)	349, 376, 747, 799, 941, 1210, 1232, 1428, 1753
Bst2BI	CACGAG	1 cut(s)	23
Bst2UI	CCWGG	4 cut(s)	356, 404, 660, 1383
Bst4CI	ACNGT	3 cut(s)	451, 626, 1591
Bst6I	CTCTTC	3 cut(s)	765, 909, 1695
BstACI	GRCGYC	2 cut(s)	165, 949
BstBAI	YACGTR	2 cut(s)	1124, 1130
BstC8I	GCNNGC	1 cut(s)	391
BstDEI	CTNAG	2 cut(s)	732, 1011
BstDSI	CCRYGG	3 cut(s)	334, 349, 1753
BstEII	GGTNACC	1 cut(s)	1757
BstF5I	GGATG	8 cut(s)	43, 94, 129, 472, 1376, 1630, 1658, 1749
BstFNI	CGCG	1 cut(s)	336
BstH2I	RGCGCY	1 cut(s)	168
BstHHI	GCGC	3 cut(s)	167, 395, 1094
BstMAI	GTCTC	3 cut(s)	135, 940, 1256
BstMWI	GCNNNNNNNGC	5 cut(s)	171, 234, 390, 488, 782
BstNI	CCWGG	4 cut(s)	356, 404, 660, 1383
BstNSI	RCATGY	1 cut(s)	265
BstPI	GGTNACC	1 cut(s)	1757
BstSCI	CCNGG	4 cut(s)	354, 402, 658, 1381
BstSLI	GKGCMC	1 cut(s)	1129
BstUI	CGCG	1 cut(s)	336
BstV1I	GCAGC	2 cut(s)	730, 1294
BstV2I	GAAGAC	2 cut(s)	86, 1552
BstX2I	RGATCY	3 cut(s)	372, 1349, 1597
BstXI	CCANNNNNNTGG	1 cut(s)	996
BstYI	RGATCY	3 cut(s)	372, 1349, 1597
BsuI	GTATCC	1 cut(s)	717
BsuRI	GGCC	7 cut(s)	143, 312, 354, 498, 920, 934, 1381
BtgI	CCRYGG	3 cut(s)	334, 349, 1753
BtgZI	GCGATG	1 cut(s)	184
BtsCI	GGATG	8 cut(s)	43, 94, 129, 472, 1376, 1630, 1658, 1749
BtsIMutI	CAGTG	3 cut(s)	447, 860, 1163
BveI	ACCTGC	2 cut(s)	111, 260
Cac8I	GCNNGC	1 cut(s)	391
CciI	TCATGA	1 cut(s)	1186
CfoI	GCGC	3 cut(s)	167, 395, 1094
Cfr10I	RCCGGY	2 cut(s)	167, 557
Cfr13I	GGNCC	4 cut(s)	933, 1042, 1379, 1705
Cfr42I	CCGCGG	1 cut(s)	337
CseI	GACGC	2 cut(s)	217, 957
CsiI	ACCWGGT	1 cut(s)	402
Csp6I	GTAC	3 cut(s)	1048, 1443, 1666
CviQI	GTAC	3 cut(s)	1048, 1443, 1666
DdeI	CTNAG	2 cut(s)	732, 1011
DinI	GGCGCC	1 cut(s)	166
DrdI	GACNNNNNNGTC	1 cut(s)	301
DseDI	GACNNNNNNGTC	1 cut(s)	301
EaeI	YGGCCR	1 cut(s)	352
Eam1104I	CTCTTC	3 cut(s)	765, 909, 1695
EarI	CTCTTC	3 cut(s)	765, 909, 1695
EciI	GGCGGA	1 cut(s)	50
Eco130I	CCWWGG	9 cut(s)	349, 376, 747, 799, 941, 1210, 1232, 1428, 1753
Eco47I	GGWCC	2 cut(s)	1042, 1705
Eco57I	CTGAAG	1 cut(s)	1238
Eco72I	CACGTG	2 cut(s)	1124, 1130
Eco88I	CYCGRG	1 cut(s)	1670
Eco91I	GGTNACC	1 cut(s)	1757
EcoO109I	RGGNCCY	2 cut(s)	933, 1042
EcoO65I	GGTNACC	1 cut(s)	1757
EcoRI	GAATTC	1 cut(s)	792
EcoRII	CCWGG	4 cut(s)	354, 402, 658, 1381
EcoT14I	CCWWGG	9 cut(s)	349, 376, 747, 799, 941, 1210, 1232, 1428, 1753
EcoT22I	ATGCAT	1 cut(s)	246
EgeI	GGCGCC	1 cut(s)	166
EheI	GGCGCC	1 cut(s)	166
ErhI	CCWWGG	9 cut(s)	349, 376, 747, 799, 941, 1210, 1232, 1428, 1753
Esp3I	CGTCTC	1 cut(s)	940
FalI	AAGNNNNNCTT	2 cut(s)	1216, 1248
FaqI	GGGAC	3 cut(s)	633, 1055, 1145
FauNDI	CATATG	2 cut(s)	427, 1087
FbaI	TGATCA	1 cut(s)	570
FblI	GTMKAC	1 cut(s)	270
Fnu4HI	GCNGC	4 cut(s)	337, 382, 744, 1283
FokI	GGATG	8 cut(s)	50, 101, 136, 459, 1383, 1637, 1665, 1736
Fsp4HI	GCNGC	4 cut(s)	337, 382, 744, 1283
FspBI	CTAG	3 cut(s)	1605, 1686, 1786
GlaI	GCGC	3 cut(s)	166, 394, 1093
GluI	GCNGC	4 cut(s)	337, 382, 744, 1283
GsaI	CCCAGC	3 cut(s)	1042, 1289, 1568
GsuI	CTGGAG	5 cut(s)	128, 275, 299, 492, 1404
HaeII	RGCGCY	1 cut(s)	168
HaeIII	GGCC	7 cut(s)	143, 312, 354, 498, 920, 934, 1381
HapII	CCGG	3 cut(s)	140, 168, 558
HgaI	GACGC	2 cut(s)	217, 957
HhaI	GCGC	3 cut(s)	167, 395, 1094
Hin1I	GRCGYC	2 cut(s)	165, 949
Hin6I	GCGC	3 cut(s)	165, 393, 1092
HinP1I	GCGC	3 cut(s)	165, 393, 1092
HincII	GTYRAC	3 cut(s)	271, 619, 1453
HindII	GTYRAC	3 cut(s)	271, 619, 1453
HinfI	GANTC	7 cut(s)	512, 639, 898, 1070, 1355, 1557, 1679
HpaII	CCGG	3 cut(s)	140, 168, 558
HphI	GGTGA	7 cut(s)	19, 241, 443, 788, 1079, 1350, 1769
Hpy166II	GTNNAC	5 cut(s)	271, 619, 1127, 1396, 1453
Hpy188I	TCNGA	7 cut(s)	78, 123, 372, 419, 782, 1240, 1354
Hpy8I	GTNNAC	5 cut(s)	271, 619, 1127, 1396, 1453
Hpy99I	CGWCG	2 cut(s)	233, 272
HpyAV	CCTTC	5 cut(s)	40, 154, 1092, 1496, 1615
HpyCH4III	ACNGT	3 cut(s)	451, 626, 1591
HpyCH4IV	ACGT	2 cut(s)	1123, 1129
HpyCH4V	TGCA	9 cut(s)	214, 244, 255, 714, 816, 850, 956, 1127, 1461
HpyF10VI	GCNNNNNNNGC	5 cut(s)	171, 234, 390, 488, 782
HpyF3I	CTNAG	2 cut(s)	732, 1011
HpySE526I	ACGT	2 cut(s)	1123, 1129
Hsp92I	GRCGYC	2 cut(s)	165, 949
HspAI	GCGC	3 cut(s)	165, 393, 1092
KasI	GGCGCC	1 cut(s)	164
Ksp22I	TGATCA	1 cut(s)	570
KspI	CCGCGG	1 cut(s)	337
LmnI	GCTCC	1 cut(s)	179
Lsp1109I	GCAGC	2 cut(s)	730, 1294
LweI	GCATC	5 cut(s)	231, 253, 469, 544, 859
MabI	ACCWGGT	1 cut(s)	402
MaeI	CTAG	3 cut(s)	1605, 1686, 1786
MaeII	ACGT	2 cut(s)	1123, 1129
MaeIII	GTNAC	6 cut(s)	406, 431, 736, 1130, 1546, 1757
MflI	RGATCY	3 cut(s)	372, 1349, 1597
MhlI	GDGCHC	1 cut(s)	1129
MlsI	TGGCCA	1 cut(s)	354
MluNI	TGGCCA	1 cut(s)	354
Mly113I	GGCGCC	1 cut(s)	165
MlyI	GAGTC	3 cut(s)	521, 1566, 1673
MmeI	TCCRAC	4 cut(s)	330, 1348, 1490, 1606
Mox20I	TGGCCA	1 cut(s)	354
Mph1103I	ATGCAT	1 cut(s)	246
MroXI	GAANNNNTTC	2 cut(s)	981, 1182
MscI	TGGCCA	1 cut(s)	354
MseI	TTAA	5 cut(s)	675, 759, 972, 1296, 1494
MslI	CAYNNNNRTG	3 cut(s)	260, 477, 698
Msp20I	TGGCCA	1 cut(s)	354
MspA1I	CMGCKG	3 cut(s)	336, 731, 1285
MspI	CCGG	3 cut(s)	140, 168, 558
MspR9I	CCNGG	4 cut(s)	356, 404, 660, 1383
MvaI	CCWGG	4 cut(s)	356, 404, 660, 1383
MvnI	CGCG	1 cut(s)	336
MwoI	GCNNNNNNNGC	5 cut(s)	171, 234, 390, 488, 782
NarI	GGCGCC	1 cut(s)	165
NcoI	CCATGG	2 cut(s)	349, 1753
NdeI	CATATG	2 cut(s)	427, 1087
NlaIV	GGNNCC	5 cut(s)	166, 175, 753, 1043, 1599
NmuCI	GTSAC	4 cut(s)	406, 431, 1130, 1757
NsiI	ATGCAT	1 cut(s)	246
NspI	RCATGY	1 cut(s)	265
PagI	TCATGA	1 cut(s)	1186
PaqCI	CACCTGC	1 cut(s)	260
PcsI	WCGNNNNNNNCGW	1 cut(s)	276
PdmI	GAANNNNTTC	2 cut(s)	981, 1182
PfeI	GAWTC	4 cut(s)	639, 898, 1070, 1355
PflFI	GACNNNGTC	1 cut(s)	404
PflMI	CCANNNNNTGG	3 cut(s)	350, 1038, 1367
PfoI	TCCNGGA	1 cut(s)	658
PkrI	GCNGC	4 cut(s)	338, 383, 745, 1284
PleI	GAGTC	3 cut(s)	520, 1565, 1673
PluTI	GGCGCC	1 cut(s)	168
PmaCI	CACGTG	2 cut(s)	1124, 1130
PmlI	CACGTG	2 cut(s)	1124, 1130
PpsI	GAGTC	3 cut(s)	520, 1565, 1673
Ppu21I	YACGTR	2 cut(s)	1124, 1130
PpuMI	RGGWCCY	1 cut(s)	1042
PshBI	ATTAAT	1 cut(s)	675
Psp5II	RGGWCCY	1 cut(s)	1042
Psp6I	CCWGG	4 cut(s)	354, 402, 658, 1381
PspCI	CACGTG	2 cut(s)	1124, 1130
PspEI	GGTNACC	1 cut(s)	1757
PspFI	CCCAGC	3 cut(s)	1038, 1285, 1564
PspGI	CCWGG	4 cut(s)	354, 402, 658, 1381
PspN4I	GGNNCC	5 cut(s)	166, 175, 753, 1043, 1599
PspPI	GGNCC	4 cut(s)	933, 1042, 1379, 1705
PspPPI	RGGWCCY	1 cut(s)	1042
PsuI	RGATCY	3 cut(s)	372, 1349, 1597
PsyI	GACNNNGTC	1 cut(s)	404
PvuII	CAGCTG	2 cut(s)	731, 1285
RsaI	GTAC	3 cut(s)	1049, 1444, 1667
RsaNI	GTAC	3 cut(s)	1048, 1443, 1666
RseI	CAYNNNNRTG	3 cut(s)	260, 477, 698
SacII	CCGCGG	1 cut(s)	337
SalI	GTCGAC	1 cut(s)	269
SaqAI	TTAA	5 cut(s)	675, 759, 972, 1296, 1494
SatI	GCNGC	4 cut(s)	337, 382, 744, 1283
Sau96I	GGNCC	4 cut(s)	933, 1042, 1379, 1705
SchI	GAGTC	3 cut(s)	521, 1566, 1673
ScrFI	CCNGG	4 cut(s)	356, 404, 660, 1383
SduI	GDGCHC	1 cut(s)	1129
SexAI	ACCWGGT	1 cut(s)	402
SfaNI	GCATC	5 cut(s)	231, 253, 469, 544, 859
SfoI	GGCGCC	1 cut(s)	166
Sfr303I	CCGCGG	1 cut(s)	337
SgrAI	CRCCGGYG	1 cut(s)	167
SgrBI	CCGCGG	1 cut(s)	337
SinI	GGWCC	2 cut(s)	1042, 1705
SmiMI	CAYNNNNRTG	3 cut(s)	260, 477, 698
SmlI	CTYRAG	1 cut(s)	539
SmoI	CTYRAG	1 cut(s)	539
SsiI	CCGC	7 cut(s)	35, 180, 334, 336, 381, 1060, 1544
SspDI	GGCGCC	1 cut(s)	164
SspMI	CTAG	3 cut(s)	1605, 1686, 1786
StyD4I	CCNGG	4 cut(s)	354, 402, 658, 1381
StyI	CCWWGG	9 cut(s)	349, 376, 747, 799, 941, 1210, 1232, 1428, 1753
TaaI	ACNGT	3 cut(s)	451, 626, 1591
TaiI	ACGT	2 cut(s)	1126, 1132
TaqI	TCGA	7 cut(s)	12, 63, 231, 270, 279, 399, 1245
TauI	GCSGC	2 cut(s)	339, 384
TfiI	GAWTC	4 cut(s)	639, 898, 1070, 1355
Tru1I	TTAA	5 cut(s)	675, 759, 972, 1296, 1494
Tru9I	TTAA	5 cut(s)	675, 759, 972, 1296, 1494
TscAI	CASTG	3 cut(s)	454, 867, 1170
TseFI	GTSAC	4 cut(s)	406, 431, 1130, 1757
TseI	GCWGC	2 cut(s)	743, 1282
Tsp45I	GTSAC	4 cut(s)	406, 431, 1130, 1757
TspDTI	ATGAA	7 cut(s)	17, 682, 783, 911, 1074, 1102, 1175
TspGWI	ACGGA	1 cut(s)	98
TspRI	CASTG	3 cut(s)	454, 867, 1170
Tth111I	GACNNNGTC	1 cut(s)	404
Van91I	CCANNNNNTGG	3 cut(s)	350, 1038, 1367
VneI	GTGCAC	1 cut(s)	1125
VpaK11BI	GGWCC	2 cut(s)	1042, 1705
VspI	ATTAAT	1 cut(s)	675
XapI	RAATTY	4 cut(s)	611, 792, 1193, 1202
XceI	RCATGY	1 cut(s)	265
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1059, 1368, 1435
XmiI	GTMKAC	1 cut(s)	270
XmnI	GAANNNNTTC	2 cut(s)	981, 1182
XspI	CTAG	3 cut(s)	1605, 1686, 1786
Zsp2I	ATGCAT	1 cut(s)	246

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.