Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0011051

Physical Location & Seq

Genomic Coordinates Reverse (-)

54656 .. 58641

Gene Structure 3986 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1001200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGAAGGTGATAGAGAAGATCCAGGAGTCATTGGTGGATGAGAAGAAGGTGGTCTTCTCCTTCGAGTTCTTCCCGCCAAAGACGGAGGATGGCGTGGAGAACCTGTTGGAGAGGTTGGATCGGATGGTGGCCCACGGCCCGGCCTTCTGCGACATAACCTGGGGTGCCGGTGGCTCCACGGCGGATCTGACGCTGGACATTGCCAACAAGATGCAGAACCTCATCTGCGTGGAGACCATGATGCACCTCACCTGCACCAACATGCCCGTCGACAAGATCGACCACGCCCTCCAAACCATCAAGTTCAACGGCCTCCAGAACGTTCTTGCTCTCCGGGGCGACCCGCCCCATGGCCAGGACAAGTTTGTTCAGATCCAAGGAGGCTTTGCCTGTGCCCTGGACCTGGTCACCCATATCAGAGCCAAATATGGTGACTACTTTGGCGTCACTGTTGCTGGTTATCCAGAGGCTCACCCAGACGCCATTGCAGCTGATGGCCTCGCCTCTCCAGAAGCATATCAGAGTGATCTATTTTATTTGAAGAGAAAGGTTGATGCTGGCGCCGATTTCATTGTTACTCAGTTATTCTACGACACTGATGTTTTCCTCAAATTCGTGAATGACTGTCGCCAAATTGGAATTACTTGTCCCATTGTTCCTGGAATTATGCCCATTAATAACTACAAGGGTTTCATACGCATGACTGGCTTCTGCAAAACAAAGATACCAGCTGAGGTTACTGCTGCCTTGGAGCCTATCAAAGACAATGAAGAAGCTGTGAGATTATACGGAATTCACCTTGGAACCGAGATGTGCAAGAAGATTTTGGCTCATGGGATTAGGACATTGCATCTTTATACTCTCAACATGGAGAAATCTGCATTGGCTATATTAACGAATCTTGGTCTGATTGAAGAGTCCAAAATATCAAGGCCCTTACCTTGGAGACGCCCTGCAAATGTTTTCCGTGTTAAAGAAGATGTTCGTCCAATTTTCTGGGCTAATCGTCCCAAGAGCTACATATCAAGAACCGTAGGCTGGGACATGTACCCACATGGGCGGTGGGGTGATTCCGGTAACCCTTCATATGGAGCACTGACTGATTATCAGTTCATGCGGCCACGTGCACGTGACAAGAAACTTGTTGAGGAATGGGTTGTCCCATTGAAGAGCATTGAAGATATCTACGAGAAATTTAAGAAGTTATGCCTTGGAAAATTGAGAAGCAGCCCTTGGTCTGAACTAGATGGGCTTCAGCCAGAGACGAGGATCATAAACGAGTTGCTAGGAAAAATTAACACTAAAGGTTTCCTTACCATCAACAGCCAACCAGCGGTAAATGGGGAAAGATCTGACTCGCCGTCTGTTGGGTGGGGTGGCCCTGGAGGATATGTTTATCAGAAAGCCTATCTAGAGTTTTTCTGTTCGAAGGAGAAGTTGGATGCTCTTGTTGAAAAATGCAAGGCTCTTCCATCTCTAACTTACATGGCCGTGAACAAAGACGGGAGCTGGATATCTAATATTGGCCAGACTGATGTGAATGCAGTAACATGGGGCGTCTTTCCAGCAAGGGAGATAATCCAACCAACTGTCGTGGATCCTACGAGTTTTATAGTTTGGAAGGACGAGGCATTTGAAATCTGGTCAAGAGGATGGGCCCGCTTATACTCTGAGGGTGACTCATCTAGGAAATTGCTCGAAGAGGTGCAGAGCAGCTACTTTTTGGTCAGTTTGGTAGATAATGACTACATCCACGGTGATGTTTTTGCTGTTTTTGCTGATTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004489 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006730 GO:0008150 GO:0008152 GO:0009987 GO:0016491 GO:0016645 GO:0016646 GO:0044237 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0055114

KEGG Pathways

Metabolic & Signaling

ko00670 ko00720 ko01100 ko01120 ko01200 ko01523 map00670 map00720 map01100 map01120 map01200 map01523

Pfam Domains

Protein Families

MTHFR

Protein Analysis

596

Amino Acids

66.94

Weight (kDa)

5.87

Isoelectric Point (pI)

38.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTHFR	PF02219	10 - 302	2.2e-120	Methylenetetrahydrofolate reductase
MTHFR_C	PF21895	311 - 585	7.6e-92	MTHFR, SAM-binding regulatory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011051)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44160 AT3G59970 AT3G59970
fragaria_vesca	FvH4_1g00040 FvH4_1g00040
malus_domestica	MD00G1145100.v1.1 MD02G1001200.v1.1 MD15G1141700.v1.1
prunus_persica	Prupe.7G271500_v2.0.a1
pyrus_communis	pycom15g12800
rosa_chinensis	RchiOBHm_Chr2g0084261
rosa_laevigata	RLG00000015604
rosa_multiflora	Rmu_sc0003129.1_g000016
rosa_roxburghii	Rroxscaffold_2G00156200
rosa_rugosa	Rorug01G0452200
rosa_samantha	Rh2AG000600 Rh2BG000500 Rh2CG000400 Rh2DG000600
rosa_wichuraiana	Rw2G000040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	260
Acc36I	ACCTGC	1 cut(s)	260
AccB1I	GGYRCC	2 cut(s)	164, 560
AccI	GTMKAC	1 cut(s)	270
AciI	CCGC	7 cut(s)	74, 182, 344, 1060, 1117, 1334, 1660
AclI	AACGTT	1 cut(s)	321
AclWI	GGATC	7 cut(s)	13, 126, 192, 367, 1277, 1592, 1605
AcoI	YGGCCR	4 cut(s)	352, 1118, 1488, 1525
AcsI	RAATTY	3 cut(s)	611, 792, 1193
AcuI	CTGAAG	1 cut(s)	1238
AcvI	CACGTG	2 cut(s)	1124, 1130
AcyI	GRCGYC	5 cut(s)	444, 480, 561, 949, 1557
AfaI	GTAC	1 cut(s)	1049
AfiI	CCNNNNNNNGG	9 cut(s)	139, 350, 355, 403, 942, 1038, 1088, 1333, 1367
AflIII	ACRYGT	1 cut(s)	1044
AgsI	TTSAA	7 cut(s)	307, 541, 914, 1168, 1178, 1454, 1637
AhdI	GACNNNNNGTC	1 cut(s)	1360
AjnI	CCWGG	7 cut(s)	21, 158, 354, 396, 402, 658, 1381
AjuI	GAANNNNNNNTTGG	6 cut(s)	866, 898, 1216, 1248, 1421, 1453
AluBI	AGCT	6 cut(s)	491, 731, 776, 1017, 1509, 1716
AluI	AGCT	6 cut(s)	491, 731, 776, 1017, 1509, 1716
Alw21I	GWGCWC	2 cut(s)	1096, 1129
Alw26I	GTCTC	3 cut(s)	227, 940, 1256
Alw44I	GTGCAC	1 cut(s)	1125
AlwI	GGATC	7 cut(s)	13, 126, 192, 367, 1277, 1592, 1605
ApaI	GGGCCC	1 cut(s)	1660
ApaLI	GTGCAC	1 cut(s)	1125
ApeKI	GCWGC	4 cut(s)	488, 743, 1227, 1713
ApoI	RAATTY	3 cut(s)	611, 792, 1193
ArsI	GACNNNNNNTTYG	2 cut(s)	1616, 1648
AseI	ATTAAT	1 cut(s)	675
Asp700I	GAANNNNTTC	1 cut(s)	981
AspLEI	GCGC	1 cut(s)	563
AspS9I	GGNCC	7 cut(s)	130, 137, 400, 933, 1379, 1656, 1657
AsuC2I	CCSGG	2 cut(s)	140, 335
AsuHPI	GGTGA	9 cut(s)	19, 241, 400, 443, 464, 788, 1079, 1688, 1769
AsuII	TTCGAA	1 cut(s)	1427
AvaII	GGWCC	1 cut(s)	400
BaeGI	GKGCMC	3 cut(s)	397, 1129, 1660
BalI	TGGCCA	2 cut(s)	354, 1527
BamHI	GGATCC	1 cut(s)	1597
BanI	GGYRCC	2 cut(s)	164, 560
BanII	GRGCYC	1 cut(s)	1660
BbrPI	CACGTG	2 cut(s)	1124, 1130
BbsI	GAAGAC	1 cut(s)	46
Bbv12I	GWGCWC	2 cut(s)	1096, 1129
BbvCI	CCTCAGC	1 cut(s)	732
BbvI	GCAGC	4 cut(s)	500, 730, 1239, 1725
BccI	CCATC	8 cut(s)	83, 118, 305, 488, 1241, 1325, 1480, 1647
BceAI	ACGGC	5 cut(s)	151, 195, 325, 1345, 1475
BciT130I	CCWGG	7 cut(s)	23, 160, 356, 398, 404, 660, 1383
BcnI	CCSGG	2 cut(s)	140, 335
BcoDI	GTCTC	3 cut(s)	227, 940, 1256
BfaI	CTAG	4 cut(s)	1244, 1286, 1412, 1686
BfoI	RGCGCY	1 cut(s)	564
BfuAI	ACCTGC	1 cut(s)	260
BglII	AGATCT	1 cut(s)	1349
BisI	GCNGC	5 cut(s)	489, 744, 1118, 1228, 1714
BlsI	GCNGC	5 cut(s)	490, 745, 1119, 1229, 1715
Bme1390I	CCNGG	9 cut(s)	23, 140, 160, 335, 356, 398, 404, 660, 1383
Bme18I	GGWCC	1 cut(s)	400
BmeRI	GACNNNNNGTC	1 cut(s)	1360
BmgT120I	GGNCC	7 cut(s)	130, 137, 400, 933, 1379, 1656, 1657
BmiI	GGNNCC	7 cut(s)	166, 175, 562, 753, 805, 1599, 1658
BmrFI	CCNGG	9 cut(s)	23, 140, 160, 335, 356, 398, 404, 660, 1383
BmsI	GCATC	5 cut(s)	201, 231, 544, 859, 1432
BpiI	GAAGAC	1 cut(s)	46
BplI	GAGNNNNNCTC	2 cut(s)	1664, 1696
BpmI	CTGGAG	3 cut(s)	299, 492, 1404
Bpu10I	CCTNAGC	1 cut(s)	732
Bpu14I	TTCGAA	1 cut(s)	1427
BpuMI	CCSGG	2 cut(s)	140, 335
BsaAI	YACGTR	2 cut(s)	1124, 1130
BsaHI	GRCGYC	5 cut(s)	444, 480, 561, 949, 1557
BsaI	GGTCTC	1 cut(s)	227
BsaWI	WCCGGW	1 cut(s)	1073
BsaXI	ACNNNNNCTCC	4 cut(s)	89, 119, 1377, 1407
Bsc4I	CCNNNNNNNGG	9 cut(s)	139, 350, 355, 403, 942, 1038, 1088, 1333, 1367
Bse118I	RCCGGY	1 cut(s)	167
Bse1I	ACTGG	1 cut(s)	709
Bse3DI	GCAATG	3 cut(s)	198, 483, 845
BseBI	CCWGG	7 cut(s)	23, 160, 356, 398, 404, 660, 1383
BseGI	GGATG	6 cut(s)	43, 94, 129, 1447, 1658, 1749
BseLI	CCNNNNNNNGG	9 cut(s)	139, 350, 355, 403, 942, 1038, 1088, 1333, 1367
BseMI	GCAATG	3 cut(s)	198, 483, 845
BseMII	CTCAG	3 cut(s)	593, 723, 1662
BseNI	ACTGG	1 cut(s)	709
BseSI	GKGCMC	3 cut(s)	397, 1129, 1660
BseXI	GCAGC	4 cut(s)	500, 730, 1239, 1725
BseYI	CCCAGC	1 cut(s)	1038
BsgI	GTGCAG	2 cut(s)	238, 1727
BshNI	GGYRCC	2 cut(s)	164, 560
BsiHKAI	GWGCWC	2 cut(s)	1096, 1129
BsiSI	CCGG	4 cut(s)	140, 168, 334, 1074
BslFI	GGGAC	4 cut(s)	633, 993, 1055, 1145
BslI	CCNNNNNNNGG	9 cut(s)	139, 350, 355, 403, 942, 1038, 1088, 1333, 1367
BsmAI	GTCTC	3 cut(s)	227, 940, 1256
BsmBI	CGTCTC	2 cut(s)	940, 1256
BsmFI	GGGAC	4 cut(s)	633, 993, 1055, 1145
BsmI	GAATGC	1 cut(s)	1546
Bso31I	GGTCTC	1 cut(s)	227
Bsp119I	TTCGAA	1 cut(s)	1427
Bsp120I	GGGCCC	1 cut(s)	1656
Bsp1286I	GDGCHC	4 cut(s)	397, 1096, 1129, 1660
Bsp143I	GATC	9 cut(s)	18, 118, 184, 276, 372, 526, 1269, 1349, 1597
Bsp19I	CCATGG	1 cut(s)	349
BspACI	CCGC	7 cut(s)	74, 182, 344, 1060, 1117, 1334, 1660
BspCNI	CTCAG	3 cut(s)	592, 724, 1663
BspLI	GGNNCC	7 cut(s)	166, 175, 562, 753, 805, 1599, 1658
BspMI	ACCTGC	1 cut(s)	260
BspPI	GGATC	7 cut(s)	13, 126, 192, 367, 1277, 1592, 1605
BspQI	GCTCTTC	2 cut(s)	1163, 1473
BspT104I	TTCGAA	1 cut(s)	1427
BspT107I	GGYRCC	2 cut(s)	164, 560
BspTNI	GGTCTC	1 cut(s)	227
BsrDI	GCAATG	3 cut(s)	198, 483, 845
BsrFI	RCCGGY	1 cut(s)	167
BsrI	ACTGG	1 cut(s)	709
BssAI	RCCGGY	1 cut(s)	167
BssMI	GATC	9 cut(s)	18, 118, 184, 276, 372, 526, 1269, 1349, 1597
BssNI	GRCGYC	5 cut(s)	444, 480, 561, 949, 1557
BssT1I	CCWWGG	7 cut(s)	349, 376, 747, 799, 941, 1210, 1232
Bst2UI	CCWGG	7 cut(s)	23, 160, 356, 398, 404, 660, 1383
Bst4CI	ACNGT	5 cut(s)	451, 626, 1033, 1591, 1757
Bst6I	CTCTTC	5 cut(s)	536, 909, 1163, 1473, 1695
BstACI	GRCGYC	5 cut(s)	444, 480, 561, 949, 1557
BstBAI	YACGTR	2 cut(s)	1124, 1130
BstBI	TTCGAA	1 cut(s)	1427
BstC8I	GCNNGC	2 cut(s)	559, 1660
BstDEI	CTNAG	3 cut(s)	579, 732, 1671
BstDSI	CCRYGG	4 cut(s)	133, 177, 349, 1753
BstEII	GGTNACC	2 cut(s)	406, 1076
BstF5I	GGATG	6 cut(s)	43, 94, 129, 1447, 1658, 1749
BstH2I	RGCGCY	1 cut(s)	564
BstHHI	GCGC	1 cut(s)	563
BstKTI	GATC	9 cut(s)	21, 121, 187, 279, 375, 529, 1272, 1352, 1600
BstMAI	GTCTC	3 cut(s)	227, 940, 1256
BstMBI	GATC	9 cut(s)	18, 118, 184, 276, 372, 526, 1269, 1349, 1597
BstMWI	GCNNNNNNNGC	3 cut(s)	488, 705, 1775
BstNI	CCWGG	7 cut(s)	23, 160, 356, 398, 404, 660, 1383
BstNSI	RCATGY	2 cut(s)	265, 1048
BstPI	GGTNACC	2 cut(s)	406, 1076
BstSCI	CCNGG	9 cut(s)	21, 138, 158, 333, 354, 396, 402, 658, 1381
BstSLI	GKGCMC	3 cut(s)	397, 1129, 1660
BstV1I	GCAGC	4 cut(s)	500, 730, 1239, 1725
BstV2I	GAAGAC	1 cut(s)	46
BstX2I	RGATCY	5 cut(s)	18, 184, 372, 1349, 1597
BstXI	CCANNNNNNTGG	1 cut(s)	996
BstYI	RGATCY	5 cut(s)	18, 184, 372, 1349, 1597
BtgI	CCRYGG	4 cut(s)	133, 177, 349, 1753
BtsCI	GGATG	6 cut(s)	43, 94, 129, 1447, 1658, 1749
BtsIMutI	CAGTG	3 cut(s)	447, 594, 1094
BveI	ACCTGC	1 cut(s)	260
Cac8I	GCNNGC	2 cut(s)	559, 1660
CfoI	GCGC	1 cut(s)	563
Cfr10I	RCCGGY	1 cut(s)	167
Cfr13I	GGNCC	7 cut(s)	130, 137, 400, 933, 1379, 1656, 1657
CseI	GACGC	5 cut(s)	199, 433, 488, 957, 1546
CsiI	ACCWGGT	1 cut(s)	402
Csp6I	GTAC	1 cut(s)	1048
CspCI	CAANNNNNGTGG	2 cut(s)	1575, 1610
CviQI	GTAC	1 cut(s)	1048
DdeI	CTNAG	3 cut(s)	579, 732, 1671
DinI	GGCGCC	1 cut(s)	562
DpnI	GATC	9 cut(s)	20, 120, 186, 278, 374, 528, 1271, 1351, 1599
DpnII	GATC	9 cut(s)	18, 118, 184, 276, 372, 526, 1269, 1349, 1597
DriI	GACNNNNNGTC	1 cut(s)	1360
EaeI	YGGCCR	4 cut(s)	352, 1118, 1488, 1525
Eam1104I	CTCTTC	5 cut(s)	536, 909, 1163, 1473, 1695
Eam1105I	GACNNNNNGTC	1 cut(s)	1360
EarI	CTCTTC	5 cut(s)	536, 909, 1163, 1473, 1695
EciI	GGCGGA	1 cut(s)	197
Eco130I	CCWWGG	7 cut(s)	349, 376, 747, 799, 941, 1210, 1232
Eco24I	GRGCYC	1 cut(s)	1660
Eco31I	GGTCTC	1 cut(s)	227
Eco32I	GATATC	2 cut(s)	1183, 1515
Eco47I	GGWCC	1 cut(s)	400
Eco57I	CTGAAG	1 cut(s)	1238
Eco72I	CACGTG	2 cut(s)	1124, 1130
Eco91I	GGTNACC	2 cut(s)	406, 1076
EcoO109I	RGGNCCY	1 cut(s)	933
EcoO65I	GGTNACC	2 cut(s)	406, 1076
EcoRI	GAATTC	1 cut(s)	792
EcoRII	CCWGG	7 cut(s)	21, 158, 354, 396, 402, 658, 1381
EcoRV	GATATC	2 cut(s)	1183, 1515
EcoT14I	CCWWGG	7 cut(s)	349, 376, 747, 799, 941, 1210, 1232
EcoT38I	GRGCYC	1 cut(s)	1660
EgeI	GGCGCC	1 cut(s)	562
EheI	GGCGCC	1 cut(s)	562
ErhI	CCWWGG	7 cut(s)	349, 376, 747, 799, 941, 1210, 1232
Esp3I	CGTCTC	2 cut(s)	940, 1256
FalI	AAGNNNNNCTT	6 cut(s)	38, 70, 1216, 1248, 1296, 1328
FaqI	GGGAC	4 cut(s)	633, 993, 1055, 1145
FauI	CCCGC	3 cut(s)	81, 351, 1667
FauNDI	CATATG	1 cut(s)	1087
FblI	GTMKAC	1 cut(s)	270
Fnu4HI	GCNGC	5 cut(s)	489, 744, 1118, 1228, 1714
FokI	GGATG	6 cut(s)	50, 101, 136, 1454, 1665, 1736
FriOI	GRGCYC	1 cut(s)	1660
Fsp4HI	GCNGC	5 cut(s)	489, 744, 1118, 1228, 1714
FspBI	CTAG	4 cut(s)	1244, 1286, 1412, 1686
GlaI	GCGC	1 cut(s)	562
GluI	GCNGC	5 cut(s)	489, 744, 1118, 1228, 1714
GsaI	CCCAGC	1 cut(s)	1042
GsuI	CTGGAG	3 cut(s)	299, 492, 1404
HaeII	RGCGCY	1 cut(s)	564
HapII	CCGG	4 cut(s)	140, 168, 334, 1074
HgaI	GACGC	5 cut(s)	199, 433, 488, 957, 1546
HhaI	GCGC	1 cut(s)	563
Hin1I	GRCGYC	5 cut(s)	444, 480, 561, 949, 1557
Hin6I	GCGC	1 cut(s)	561
HinP1I	GCGC	1 cut(s)	561
HincII	GTYRAC	1 cut(s)	271
HindII	GTYRAC	1 cut(s)	271
HinfI	GANTC	6 cut(s)	26, 898, 917, 1070, 1355, 1679
HpaII	CCGG	4 cut(s)	140, 168, 334, 1074
HphI	GGTGA	9 cut(s)	19, 241, 400, 443, 464, 788, 1079, 1688, 1769
Hpy166II	GTNNAC	3 cut(s)	271, 1127, 1495
Hpy188III	TCNNGA	7 cut(s)	316, 464, 509, 616, 1026, 1412, 1647
Hpy8I	GTNNAC	3 cut(s)	271, 1127, 1495
Hpy99I	CGWCG	1 cut(s)	272
HpyAV	CCTTC	6 cut(s)	40, 70, 154, 1092, 1423, 1615
HpyCH4III	ACNGT	5 cut(s)	451, 626, 1033, 1591, 1757
HpyCH4IV	ACGT	3 cut(s)	321, 1123, 1129
HpyF10VI	GCNNNNNNNGC	3 cut(s)	488, 705, 1775
HpyF3I	CTNAG	3 cut(s)	579, 732, 1671
HpySE526I	ACGT	3 cut(s)	321, 1123, 1129
Hsp92I	GRCGYC	5 cut(s)	444, 480, 561, 949, 1557
HspAI	GCGC	1 cut(s)	561
KasI	GGCGCC	1 cut(s)	560
Kzo9I	GATC	9 cut(s)	18, 118, 184, 276, 372, 526, 1269, 1349, 1597
LguI	GCTCTTC	2 cut(s)	1163, 1473
LmnI	GCTCC	4 cut(s)	179, 751, 1091, 1506
Lsp1109I	GCAGC	4 cut(s)	500, 730, 1239, 1725
LweI	GCATC	5 cut(s)	201, 231, 544, 859, 1432
MabI	ACCWGGT	1 cut(s)	402
MaeI	CTAG	4 cut(s)	1244, 1286, 1412, 1686
MaeII	ACGT	3 cut(s)	321, 1123, 1129
MaeIII	GTNAC	9 cut(s)	406, 431, 445, 574, 736, 1076, 1130, 1546, 1676
MalI	GATC	9 cut(s)	20, 120, 186, 278, 374, 528, 1271, 1351, 1599
MboI	GATC	9 cut(s)	18, 118, 184, 276, 372, 526, 1269, 1349, 1597
MflI	RGATCY	5 cut(s)	18, 184, 372, 1349, 1597
MhlI	GDGCHC	4 cut(s)	397, 1096, 1129, 1660
MlsI	TGGCCA	2 cut(s)	354, 1527
MluNI	TGGCCA	2 cut(s)	354, 1527
Mly113I	GGCGCC	1 cut(s)	561
MlyI	GAGTC	4 cut(s)	35, 926, 1349, 1673
MmeI	TCCRAC	4 cut(s)	87, 96, 1419, 1606
Mox20I	TGGCCA	2 cut(s)	354, 1527
MroXI	GAANNNNTTC	1 cut(s)	981
MscI	TGGCCA	2 cut(s)	354, 1527
MseI	TTAA	5 cut(s)	675, 893, 972, 1197, 1296
MslI	CAYNNNNRTG	5 cut(s)	227, 260, 698, 1490, 1758
Msp20I	TGGCCA	2 cut(s)	354, 1527
MspA1I	CMGCKG	3 cut(s)	491, 731, 1334
MspI	CCGG	4 cut(s)	140, 168, 334, 1074
MspR9I	CCNGG	9 cut(s)	23, 140, 160, 335, 356, 398, 404, 660, 1383
Mva1269I	GAATGC	1 cut(s)	1546
MvaI	CCWGG	7 cut(s)	23, 160, 356, 398, 404, 660, 1383
MwoI	GCNNNNNNNGC	3 cut(s)	488, 705, 1775
NarI	GGCGCC	1 cut(s)	561
NciI	CCSGG	2 cut(s)	140, 335
NcoI	CCATGG	1 cut(s)	349
NdeI	CATATG	1 cut(s)	1087
NdeII	GATC	9 cut(s)	18, 118, 184, 276, 372, 526, 1269, 1349, 1597
NlaIV	GGNNCC	7 cut(s)	166, 175, 562, 753, 805, 1599, 1658
NmuCI	GTSAC	5 cut(s)	406, 431, 445, 1130, 1676
NspI	RCATGY	2 cut(s)	265, 1048
NspV	TTCGAA	1 cut(s)	1427
PaqCI	CACCTGC	1 cut(s)	260
PciI	ACATGT	1 cut(s)	1044
PciSI	GCTCTTC	2 cut(s)	1163, 1473
PcsI	WCGNNNNNNNCGW	1 cut(s)	276
PctI	GAATGC	1 cut(s)	1546
PdmI	GAANNNNTTC	1 cut(s)	981
PfeI	GAWTC	2 cut(s)	898, 1070
PflFI	GACNNNGTC	1 cut(s)	404
PfoI	TCCNGGA	2 cut(s)	21, 658
PkrI	GCNGC	5 cut(s)	490, 745, 1119, 1229, 1715
PleI	GAGTC	4 cut(s)	34, 925, 1349, 1673
PluTI	GGCGCC	1 cut(s)	564
PmaCI	CACGTG	2 cut(s)	1124, 1130
PmlI	CACGTG	2 cut(s)	1124, 1130
PpsI	GAGTC	4 cut(s)	34, 925, 1349, 1673
Ppu21I	YACGTR	2 cut(s)	1124, 1130
PscI	ACATGT	1 cut(s)	1044
PshBI	ATTAAT	1 cut(s)	675
Psp1406I	AACGTT	1 cut(s)	321
Psp6I	CCWGG	7 cut(s)	21, 158, 354, 396, 402, 658, 1381
PspCI	CACGTG	2 cut(s)	1124, 1130
PspEI	GGTNACC	2 cut(s)	406, 1076
PspFI	CCCAGC	1 cut(s)	1038
PspGI	CCWGG	7 cut(s)	21, 158, 354, 396, 402, 658, 1381
PspN4I	GGNNCC	7 cut(s)	166, 175, 562, 753, 805, 1599, 1658
PspOMI	GGGCCC	1 cut(s)	1656
PspPI	GGNCC	7 cut(s)	130, 137, 400, 933, 1379, 1656, 1657
PsuI	RGATCY	5 cut(s)	18, 184, 372, 1349, 1597
PsyI	GACNNNGTC	1 cut(s)	404
PvuII	CAGCTG	2 cut(s)	491, 731
RsaI	GTAC	1 cut(s)	1049
RsaNI	GTAC	1 cut(s)	1048
RseI	CAYNNNNRTG	5 cut(s)	227, 260, 698, 1490, 1758
SalI	GTCGAC	1 cut(s)	269
SapI	GCTCTTC	2 cut(s)	1163, 1473
SaqAI	TTAA	5 cut(s)	675, 893, 972, 1197, 1296
SatI	GCNGC	5 cut(s)	489, 744, 1118, 1228, 1714
Sau3AI	GATC	9 cut(s)	18, 118, 184, 276, 372, 526, 1269, 1349, 1597
Sau96I	GGNCC	7 cut(s)	130, 137, 400, 933, 1379, 1656, 1657
SchI	GAGTC	4 cut(s)	35, 926, 1349, 1673
ScrFI	CCNGG	9 cut(s)	23, 140, 160, 335, 356, 398, 404, 660, 1383
SduI	GDGCHC	4 cut(s)	397, 1096, 1129, 1660
SexAI	ACCWGGT	1 cut(s)	402
SfaNI	GCATC	5 cut(s)	201, 231, 544, 859, 1432
SfoI	GGCGCC	1 cut(s)	562
SfuI	TTCGAA	1 cut(s)	1427
SinI	GGWCC	1 cut(s)	400
SmiMI	CAYNNNNRTG	5 cut(s)	227, 260, 698, 1490, 1758
SsiI	CCGC	7 cut(s)	74, 182, 344, 1060, 1117, 1334, 1660
SspDI	GGCGCC	1 cut(s)	560
SspI	AATATT	1 cut(s)	1522
SspMI	CTAG	4 cut(s)	1244, 1286, 1412, 1686
StyD4I	CCNGG	9 cut(s)	21, 138, 158, 333, 354, 396, 402, 658, 1381
StyI	CCWWGG	7 cut(s)	349, 376, 747, 799, 941, 1210, 1232
TaaI	ACNGT	5 cut(s)	451, 626, 1033, 1591, 1757
TaiI	ACGT	3 cut(s)	324, 1126, 1132
TaqI	TCGA	5 cut(s)	63, 270, 279, 1427, 1698
TauI	GCSGC	1 cut(s)	1120
TfiI	GAWTC	2 cut(s)	898, 1070
Tru1I	TTAA	5 cut(s)	675, 893, 972, 1197, 1296
Tru9I	TTAA	5 cut(s)	675, 893, 972, 1197, 1296
TscAI	CASTG	3 cut(s)	454, 601, 1101
TseFI	GTSAC	5 cut(s)	406, 431, 445, 1130, 1676
TseI	GCWGC	4 cut(s)	488, 743, 1227, 1713
Tsp45I	GTSAC	5 cut(s)	406, 431, 445, 1130, 1676
TspDTI	ATGAA	6 cut(s)	17, 559, 682, 783, 1074, 1102
TspGWI	ACGGA	3 cut(s)	98, 804, 956
TspRI	CASTG	3 cut(s)	454, 601, 1101
Tth111I	GACNNNGTC	1 cut(s)	404
VneI	GTGCAC	1 cut(s)	1125
VpaK11BI	GGWCC	1 cut(s)	400
VspI	ATTAAT	1 cut(s)	675
XapI	RAATTY	3 cut(s)	611, 792, 1193
XbaI	TCTAGA	1 cut(s)	1411
XceI	RCATGY	2 cut(s)	265, 1048
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1059
XmiI	GTMKAC	1 cut(s)	270
XmnI	GAANNNNTTC	1 cut(s)	981
XspI	CTAG	4 cut(s)	1244, 1286, 1412, 1686

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.