Rh2DG000600

Methylenetetrahydrofolate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011051
Physical Location & Seq
Reverse (-)
65978 .. 70728
4751 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG000600.1

Sequence Viewer

Length: 1377 bp
ATGAAGGTGATCGACAAAATCCACGAGGCATTGGCGGATGAGAAGAAGGTGGTGTTCTCGTTCGAGTTCTTCCCTCCGAAGACGGAGGATGGGGTGGAGAACCTGCTGGAGAGAATGGATCGGATGGTGGCTCACAATCCGGCCTTCTGCGACATCACTTGGGGCGCCGGTGGCTCCACGGCCGATCTCACTCTGGACATCGCCAACAAAATGCAGAACATGATCTGCGTCGAGAGCATGATGCATCTCACCTGCACCAACATGCCCGTCGACAAGATCGACCATGCCCTCCAGACTATCAAGTCCAACGGCCTCCAGAACGTTCTTGCTCTCCGCGGCGACCCACCCCATGGCCAGGACAAGTTTGTTCAGATCCAAGGCGGCTTTGCCTGCGCCCTCGACCTGGTCACTCATATCAGAGCCACATATGGTGACTACTTTGGTATCACTGTTGCTGGTTATCCAGAGGCACATCCCGATGCCATTGAAGCCGATGGCCTCGCCACTCCAGAGTCCTATCAAAGTGATCTGGCTTATCTCAAAAGAAAGTTTGATGCTGGTGCCGATTTGATCGTCACTCAATTATTCTACGATACTGATGTATTCCTCAAATTCGTCAACGACTGTCGCCAAATTGGAATCACTTGTCCCATTGTTCCTGGAATTATGCCTATTAATAACTACAAAGGTTTCATACGCATGACTGGTTTCTGCAAAACAAAGATACCTGCTGAGGTTACTGCTGCCTTGGAACCTATTAAGGACAATGAAGAAGCTGTCAGAGCTTATGGAATTCACCTTGGAACAGAAATGTGCAAGAAGATTTTAGCTCATGGGATTAGGACATTGCATCTTTATACACTGAACATGGAGAAATCAGCATTGGCTATACTAATGAATCTTGGTTTGATTGAAGAGGCCAAAATATCAAGGCCCTTACCTTGGAGACGCCCTGCAAATGTTTTTCGTGTTAAAGAAGATGTTCGTCCAATTTTCTGGGCTAATCGTCCTAAGAGCTACATATCAAGAACCATAGGCTGGGACCTGTACCCACATGGGCGGTGGGGTGATTCTCGTAATCCTTCATATGGCGCACTAACTGATTACCAGTTCATGCGACCACGTGCACGTGACAAGAAACTTGTTGAAGAATGGGTTGTCCCACTGAAAAGTGTTGAAGATATTCATGAGAAATTTGAGAAATTCTGCCTTGGAAAGTTGAGAAGTAGCCCTTGGTCAGAACTCGATGGGCTTCAGCCAGAGACAAAGATCATAAATGAACAGCTGGGGAAAATTAACACAAAGGGTTTCCTTACCATCAACAGCCAACCAGCAATAAATGGGGAAAGATCTGATTCACCATCTGTTGGTAAGTAG

Protein Analysis

458

Amino Acids

51.49

Weight (kDa)

6.48

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTHFR PF02219 9 - 302 2.1e-121 Methylenetetrahydrofolate reductase
MTHFR_C PF21895 311 - 457 3e-38 MTHFR, SAM-binding regulatory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44160 AT3G59970 AT3G59970
fragaria_vesca FvH4_1g00040 FvH4_1g00040
malus_domestica MD00G1145100.v1.1 MD02G1001200.v1.1 MD15G1141700.v1.1
prunus_persica Prupe.7G271500_v2.0.a1
pyrus_communis pycom15g12800
rosa_chinensis RchiOBHm_Chr2g0084261
rosa_laevigata RLG00000015604
rosa_multiflora Rmu_sc0003129.1_g000016
rosa_roxburghii Rroxscaffold_2G00156200
rosa_rugosa Rorug01G0452200
rosa_samantha Rh2AG000600 Rh2BG000500 Rh2CG000400 Rh2DG000600
rosa_wichuraiana Rw2G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 260
AasI GACNNNNNNGTC 1 cut(s) 301
Acc36I ACCTGC 3 cut(s) 111, 260, 736
AccB1I GGYRCC 2 cut(s) 164, 560
AccB7I CCANNNNNTGG 3 cut(s) 350, 1038, 1367
AccI GTMKAC 1 cut(s) 270
AccII CGCG 1 cut(s) 336
AciI CCGC 5 cut(s) 35, 334, 336, 381, 1060
AclI AACGTT 1 cut(s) 321
AclWI GGATC 2 cut(s) 126, 367
AcoI YGGCCR 2 cut(s) 180, 352
AcsI RAATTY 4 cut(s) 611, 792, 1193, 1202
AcuI CTGAAG 1 cut(s) 1238
AcvI CACGTG 2 cut(s) 1124, 1130
AcyI GRCGYC 2 cut(s) 165, 949
AfaI GTAC 1 cut(s) 1049
AfiI CCNNNNNNNGG 7 cut(s) 350, 355, 403, 942, 1038, 1088, 1367
AgsI TTSAA 4 cut(s) 488, 914, 1148, 1178
AjnI CCWGG 3 cut(s) 354, 402, 658
AjuI GAANNNNNNNTTGG 4 cut(s) 866, 898, 1216, 1248
AluBI AGCT 5 cut(s) 776, 785, 830, 1017, 1285
AluI AGCT 5 cut(s) 776, 785, 830, 1017, 1285
Alw21I GWGCWC 1 cut(s) 1129
Alw26I GTCTC 2 cut(s) 940, 1256
Alw44I GTGCAC 1 cut(s) 1125
AlwI GGATC 2 cut(s) 126, 367
AoxI GGCC 7 cut(s) 141, 180, 310, 352, 496, 918, 932
ApaLI GTGCAC 1 cut(s) 1125
ApeKI GCWGC 1 cut(s) 743
ApoI RAATTY 4 cut(s) 611, 792, 1193, 1202
AseI ATTAAT 1 cut(s) 675
Asp700I GAANNNNTTC 2 cut(s) 981, 1182
AspLEI GCGC 3 cut(s) 167, 395, 1094
AspS9I GGNCC 2 cut(s) 933, 1042
AsuHPI GGTGA 6 cut(s) 19, 241, 443, 788, 1079, 1350
AvaII GGWCC 1 cut(s) 1042
BaeGI GKGCMC 1 cut(s) 1129
BaeI ACNNNNGTAYC 4 cut(s) 427, 460, 585, 618
BalI TGGCCA 1 cut(s) 354
BanI GGYRCC 2 cut(s) 164, 560
BauI CACGAG 1 cut(s) 23
BbrPI CACGTG 2 cut(s) 1124, 1130
BbsI GAAGAC 1 cut(s) 86
Bbv12I GWGCWC 1 cut(s) 1129
BbvCI CCTCAGC 1 cut(s) 732
BbvI GCAGC 1 cut(s) 730
BccI CCATC 6 cut(s) 83, 118, 488, 1241, 1325, 1369
BceAI ACGGC 2 cut(s) 195, 325
BciT130I CCWGG 3 cut(s) 356, 404, 660
BcoDI GTCTC 2 cut(s) 940, 1256
BfoI RGCGCY 1 cut(s) 168
BfuAI ACCTGC 3 cut(s) 111, 260, 736
BglII AGATCT 1 cut(s) 1349
BisI GCNGC 3 cut(s) 337, 382, 744
BlsI GCNGC 3 cut(s) 338, 383, 745
Bme1390I CCNGG 3 cut(s) 356, 404, 660
Bme18I GGWCC 1 cut(s) 1042
BmgT120I GGNCC 2 cut(s) 933, 1042
BmiI GGNNCC 5 cut(s) 166, 175, 562, 753, 1043
BmrFI CCNGG 3 cut(s) 356, 404, 660
BmsI GCATC 5 cut(s) 231, 253, 469, 544, 859
BpiI GAAGAC 1 cut(s) 86
BpmI CTGGAG 4 cut(s) 128, 275, 299, 492
Bpu10I CCTNAGC 1 cut(s) 732
BsaAI YACGTR 2 cut(s) 1124, 1130
BsaHI GRCGYC 2 cut(s) 165, 949
BsaJI CCNNGG 9 cut(s) 177, 334, 349, 376, 747, 799, 941, 1210, 1232
Bsc4I CCNNNNNNNGG 7 cut(s) 350, 355, 403, 942, 1038, 1088, 1367
Bse118I RCCGGY 1 cut(s) 167
Bse1I ACTGG 2 cut(s) 709, 1108
Bse3DI GCAATG 1 cut(s) 845
BseBI CCWGG 3 cut(s) 356, 404, 660
BseDI CCNNGG 9 cut(s) 177, 334, 349, 376, 747, 799, 941, 1210, 1232
BseGI GGATG 4 cut(s) 43, 94, 129, 472
BseLI CCNNNNNNNGG 7 cut(s) 350, 355, 403, 942, 1038, 1088, 1367
BseMI GCAATG 1 cut(s) 845
BseMII CTCAG 1 cut(s) 723
BseNI ACTGG 2 cut(s) 709, 1108
BseSI GKGCMC 1 cut(s) 1129
BseX3I CGGCCG 1 cut(s) 180
BseXI GCAGC 1 cut(s) 730
BseYI CCCAGC 2 cut(s) 1038, 1285
BsgI GTGCAG 1 cut(s) 238
Bsh1236I CGCG 1 cut(s) 336
Bsh1285I CGRYCG 1 cut(s) 183
BshFI GGCC 7 cut(s) 143, 182, 312, 354, 498, 920, 934
BshNI GGYRCC 2 cut(s) 164, 560
BsiEI CGRYCG 1 cut(s) 183
BsiHKAI GWGCWC 1 cut(s) 1129
BsiSI CCGG 2 cut(s) 140, 168
BslFI GGGAC 3 cut(s) 633, 1055, 1145
BslI CCNNNNNNNGG 7 cut(s) 350, 355, 403, 942, 1038, 1088, 1367
BsmAI GTCTC 2 cut(s) 940, 1256
BsmBI CGTCTC 1 cut(s) 940
BsmFI GGGAC 3 cut(s) 633, 1055, 1145
BsnI GGCC 7 cut(s) 143, 182, 312, 354, 498, 920, 934
Bsp1286I GDGCHC 1 cut(s) 1129
Bsp19I CCATGG 1 cut(s) 349
BspACI CCGC 5 cut(s) 35, 334, 336, 381, 1060
BspANI GGCC 7 cut(s) 143, 182, 312, 354, 498, 920, 934
BspCNI CTCAG 1 cut(s) 724
BspFNI CGCG 1 cut(s) 336
BspHI TCATGA 1 cut(s) 1186
BspLI GGNNCC 5 cut(s) 166, 175, 562, 753, 1043
BspMI ACCTGC 3 cut(s) 111, 260, 736
BspPI GGATC 2 cut(s) 126, 367
BspT107I GGYRCC 2 cut(s) 164, 560
BsrDI GCAATG 1 cut(s) 845
BsrFI RCCGGY 1 cut(s) 167
BsrI ACTGG 2 cut(s) 709, 1108
BssAI RCCGGY 1 cut(s) 167
BssECI CCNNGG 9 cut(s) 177, 334, 349, 376, 747, 799, 941, 1210, 1232
BssNI GRCGYC 2 cut(s) 165, 949
BssSI CACGAG 1 cut(s) 23
BssT1I CCWWGG 7 cut(s) 349, 376, 747, 799, 941, 1210, 1232
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 3 cut(s) 356, 404, 660
Bst4CI ACNGT 2 cut(s) 451, 626
Bst6I CTCTTC 1 cut(s) 909
BstACI GRCGYC 2 cut(s) 165, 949
BstBAI YACGTR 2 cut(s) 1124, 1130
BstC8I GCNNGC 1 cut(s) 391
BstDEI CTNAG 2 cut(s) 732, 1011
BstDSI CCRYGG 3 cut(s) 177, 334, 349
BstF5I GGATG 4 cut(s) 43, 94, 129, 472
BstFNI CGCG 1 cut(s) 336
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 3 cut(s) 167, 395, 1094
BstMAI GTCTC 2 cut(s) 940, 1256
BstMCI CGRYCG 1 cut(s) 183
BstMWI GCNNNNNNNGC 5 cut(s) 171, 234, 390, 488, 782
BstNI CCWGG 3 cut(s) 356, 404, 660
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 3 cut(s) 354, 402, 658
BstSLI GKGCMC 1 cut(s) 1129
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 730
BstV2I GAAGAC 1 cut(s) 86
BstX2I RGATCY 2 cut(s) 372, 1349
BstXI CCANNNNNNTGG 1 cut(s) 996
BstYI RGATCY 2 cut(s) 372, 1349
BstZI CGGCCG 1 cut(s) 180
BsuRI GGCC 7 cut(s) 143, 182, 312, 354, 498, 920, 934
BtgI CCRYGG 3 cut(s) 177, 334, 349
BtgZI GCGATG 1 cut(s) 184
BtsCI GGATG 4 cut(s) 43, 94, 129, 472
BtsIMutI CAGTG 3 cut(s) 447, 860, 1163
BveI ACCTGC 3 cut(s) 111, 260, 736
Cac8I GCNNGC 1 cut(s) 391
CciI TCATGA 1 cut(s) 1186
CfoI GCGC 3 cut(s) 167, 395, 1094
Cfr10I RCCGGY 1 cut(s) 167
Cfr13I GGNCC 2 cut(s) 933, 1042
Cfr42I CCGCGG 1 cut(s) 337
CseI GACGC 2 cut(s) 217, 957
CsiI ACCWGGT 1 cut(s) 402
Csp6I GTAC 1 cut(s) 1048
CviQI GTAC 1 cut(s) 1048
DdeI CTNAG 2 cut(s) 732, 1011
DinI GGCGCC 1 cut(s) 166
DrdI GACNNNNNNGTC 1 cut(s) 301
DseDI GACNNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 2 cut(s) 180, 352
EagI CGGCCG 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 909
EarI CTCTTC 1 cut(s) 909
EciI GGCGGA 1 cut(s) 50
EclXI CGGCCG 1 cut(s) 180
Eco130I CCWWGG 7 cut(s) 349, 376, 747, 799, 941, 1210, 1232
Eco47I GGWCC 1 cut(s) 1042
Eco52I CGGCCG 1 cut(s) 180
Eco57I CTGAAG 1 cut(s) 1238
Eco72I CACGTG 2 cut(s) 1124, 1130
EcoO109I RGGNCCY 2 cut(s) 933, 1042
EcoRI GAATTC 1 cut(s) 792
EcoRII CCWGG 3 cut(s) 354, 402, 658
EcoT14I CCWWGG 7 cut(s) 349, 376, 747, 799, 941, 1210, 1232
EcoT22I ATGCAT 1 cut(s) 246
EgeI GGCGCC 1 cut(s) 166
EheI GGCGCC 1 cut(s) 166
ErhI CCWWGG 7 cut(s) 349, 376, 747, 799, 941, 1210, 1232
Esp3I CGTCTC 1 cut(s) 940
FalI AAGNNNNNCTT 2 cut(s) 1216, 1248
FaqI GGGAC 3 cut(s) 633, 1055, 1145
FauNDI CATATG 2 cut(s) 427, 1087
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 3 cut(s) 337, 382, 744
FokI GGATG 4 cut(s) 50, 101, 136, 459
Fsp4HI GCNGC 3 cut(s) 337, 382, 744
GlaI GCGC 3 cut(s) 166, 394, 1093
GluI GCNGC 3 cut(s) 337, 382, 744
GsaI CCCAGC 2 cut(s) 1042, 1289
GsuI CTGGAG 4 cut(s) 128, 275, 299, 492
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 7 cut(s) 143, 182, 312, 354, 498, 920, 934
HapII CCGG 2 cut(s) 140, 168
HgaI GACGC 2 cut(s) 217, 957
HhaI GCGC 3 cut(s) 167, 395, 1094
Hin1I GRCGYC 2 cut(s) 165, 949
Hin6I GCGC 3 cut(s) 165, 393, 1092
HinP1I GCGC 3 cut(s) 165, 393, 1092
HincII GTYRAC 2 cut(s) 271, 619
HindII GTYRAC 2 cut(s) 271, 619
HinfI GANTC 5 cut(s) 512, 639, 898, 1070, 1355
HpaII CCGG 2 cut(s) 140, 168
HphI GGTGA 6 cut(s) 19, 241, 443, 788, 1079, 1350
Hpy166II GTNNAC 3 cut(s) 271, 619, 1127
Hpy188I TCNGA 7 cut(s) 78, 123, 372, 419, 782, 1240, 1354
Hpy188III TCNNGA 9 cut(s) 194, 232, 292, 316, 464, 476, 509, 1026, 1187
Hpy8I GTNNAC 3 cut(s) 271, 619, 1127
Hpy99I CGWCG 2 cut(s) 233, 272
HpyAV CCTTC 3 cut(s) 40, 154, 1092
HpyCH4III ACNGT 2 cut(s) 451, 626
HpyCH4IV ACGT 3 cut(s) 321, 1123, 1129
HpyCH4V TGCA 8 cut(s) 214, 244, 255, 714, 816, 850, 956, 1127
HpyF10VI GCNNNNNNNGC 5 cut(s) 171, 234, 390, 488, 782
HpyF3I CTNAG 2 cut(s) 732, 1011
HpySE526I ACGT 3 cut(s) 321, 1123, 1129
Hsp92I GRCGYC 2 cut(s) 165, 949
HspAI GCGC 3 cut(s) 165, 393, 1092
KasI GGCGCC 1 cut(s) 164
KspI CCGCGG 1 cut(s) 337
LmnI GCTCC 1 cut(s) 179
Lsp1109I GCAGC 1 cut(s) 730
LweI GCATC 5 cut(s) 231, 253, 469, 544, 859
MabI ACCWGGT 1 cut(s) 402
MaeII ACGT 3 cut(s) 321, 1123, 1129
MaeIII GTNAC 5 cut(s) 406, 431, 574, 736, 1130
MboII GAAGA 9 cut(s) 55, 61, 91, 782, 832, 926, 989, 1160, 1190
MflI RGATCY 2 cut(s) 372, 1349
MhlI GDGCHC 1 cut(s) 1129
MlsI TGGCCA 1 cut(s) 354
MluCI AATT 9 cut(s) 581, 611, 633, 663, 792, 990, 1193, 1202, 1293
MluNI TGGCCA 1 cut(s) 354
Mly113I GGCGCC 1 cut(s) 165
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 330
Mox20I TGGCCA 1 cut(s) 354
Mph1103I ATGCAT 1 cut(s) 246
MroXI GAANNNNTTC 2 cut(s) 981, 1182
MscI TGGCCA 1 cut(s) 354
MseI TTAA 4 cut(s) 675, 759, 972, 1296
MslI CAYNNNNRTG 3 cut(s) 260, 477, 698
Msp20I TGGCCA 1 cut(s) 354
MspA1I CMGCKG 2 cut(s) 336, 1285
MspI CCGG 2 cut(s) 140, 168
MspR9I CCNGG 3 cut(s) 356, 404, 660
MvaI CCWGG 3 cut(s) 356, 404, 660
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 5 cut(s) 171, 234, 390, 488, 782
NarI GGCGCC 1 cut(s) 165
NcoI CCATGG 1 cut(s) 349
NdeI CATATG 2 cut(s) 427, 1087
NlaIV GGNNCC 5 cut(s) 166, 175, 562, 753, 1043
NmuCI GTSAC 4 cut(s) 406, 431, 574, 1130
NsiI ATGCAT 1 cut(s) 246
NspI RCATGY 1 cut(s) 265
PagI TCATGA 1 cut(s) 1186
PaqCI CACCTGC 1 cut(s) 260
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PdmI GAANNNNTTC 2 cut(s) 981, 1182
PfeI GAWTC 4 cut(s) 639, 898, 1070, 1355
PflFI GACNNNGTC 1 cut(s) 404
PflMI CCANNNNNTGG 3 cut(s) 350, 1038, 1367
PfoI TCCNGGA 1 cut(s) 658
PkrI GCNGC 3 cut(s) 338, 383, 745
PleI GAGTC 1 cut(s) 520
PluTI GGCGCC 1 cut(s) 168
PmaCI CACGTG 2 cut(s) 1124, 1130
PmlI CACGTG 2 cut(s) 1124, 1130
PpsI GAGTC 1 cut(s) 520
Ppu21I YACGTR 2 cut(s) 1124, 1130
PpuMI RGGWCCY 1 cut(s) 1042
PshBI ATTAAT 1 cut(s) 675
Psp1406I AACGTT 1 cut(s) 321
Psp5II RGGWCCY 1 cut(s) 1042
Psp6I CCWGG 3 cut(s) 354, 402, 658
PspCI CACGTG 2 cut(s) 1124, 1130
PspFI CCCAGC 2 cut(s) 1038, 1285
PspGI CCWGG 3 cut(s) 354, 402, 658
PspN4I GGNNCC 5 cut(s) 166, 175, 562, 753, 1043
PspPI GGNCC 2 cut(s) 933, 1042
PspPPI RGGWCCY 1 cut(s) 1042
PsuI RGATCY 2 cut(s) 372, 1349
PsyI GACNNNGTC 1 cut(s) 404
PvuII CAGCTG 1 cut(s) 1285
RsaI GTAC 1 cut(s) 1049
RsaNI GTAC 1 cut(s) 1048
RseI CAYNNNNRTG 3 cut(s) 260, 477, 698
SacII CCGCGG 1 cut(s) 337
SalI GTCGAC 1 cut(s) 269
SaqAI TTAA 4 cut(s) 675, 759, 972, 1296
SatI GCNGC 3 cut(s) 337, 382, 744
Sau96I GGNCC 2 cut(s) 933, 1042
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 3 cut(s) 356, 404, 660
SduI GDGCHC 1 cut(s) 1129
SexAI ACCWGGT 1 cut(s) 402
SfaNI GCATC 5 cut(s) 231, 253, 469, 544, 859
SfoI GGCGCC 1 cut(s) 166
Sfr303I CCGCGG 1 cut(s) 337
SgrAI CRCCGGYG 1 cut(s) 167
SgrBI CCGCGG 1 cut(s) 337
SinI GGWCC 1 cut(s) 1042
SmiMI CAYNNNNRTG 3 cut(s) 260, 477, 698
Sse9I AATT 9 cut(s) 581, 611, 633, 663, 792, 990, 1193, 1202, 1293
SsiI CCGC 5 cut(s) 35, 334, 336, 381, 1060
SspDI GGCGCC 1 cut(s) 164
StyD4I CCNGG 3 cut(s) 354, 402, 658
StyI CCWWGG 7 cut(s) 349, 376, 747, 799, 941, 1210, 1232
TaaI ACNGT 2 cut(s) 451, 626
TaiI ACGT 3 cut(s) 324, 1126, 1132
TaqI TCGA 7 cut(s) 12, 63, 231, 270, 279, 399, 1245
TasI AATT 9 cut(s) 581, 611, 633, 663, 792, 990, 1193, 1202, 1293
TauI GCSGC 2 cut(s) 339, 384
TfiI GAWTC 4 cut(s) 639, 898, 1070, 1355
Tru1I TTAA 4 cut(s) 675, 759, 972, 1296
Tru9I TTAA 4 cut(s) 675, 759, 972, 1296
TscAI CASTG 3 cut(s) 454, 867, 1170
TseFI GTSAC 4 cut(s) 406, 431, 574, 1130
TseI GCWGC 1 cut(s) 743
Tsp45I GTSAC 4 cut(s) 406, 431, 574, 1130
TspDTI ATGAA 8 cut(s) 17, 682, 783, 911, 1074, 1102, 1175, 1293
TspGWI ACGGA 1 cut(s) 98
TspRI CASTG 3 cut(s) 454, 867, 1170
Tth111I GACNNNGTC 1 cut(s) 404
Van91I CCANNNNNTGG 3 cut(s) 350, 1038, 1367
VneI GTGCAC 1 cut(s) 1125
VpaK11BI GGWCC 1 cut(s) 1042
VspI ATTAAT 1 cut(s) 675
XapI RAATTY 4 cut(s) 611, 792, 1193, 1202
XceI RCATGY 1 cut(s) 265
XcmI CCANNNNNNNNNTGG 1 cut(s) 1059
XmiI GTMKAC 1 cut(s) 270
XmnI GAANNNNTTC 2 cut(s) 981, 1182
Zsp2I ATGCAT 1 cut(s) 246
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.