AT3G59970

Methylenetetrahydrofolate reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0011051
Physical Location & Seq
Forward (+)
22151164 .. 22154483
3320 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G59970.1

Sequence Viewer

Length: 1266 bp
ATGAAGGTGGTTGATAAGATCAAATCCGTGACGGAGCAAGGCCAAACAGCTTTCTCCTTCGAGTTTTTCCCACCGAAGACAGAAGATGGCGTCGAGAATCTCTTTGAACGAATGGATCGATTGGTCTCTTATGGACCTACCTTCTGCGATATCACTTGGGGTGCTGGTGGATCCACAGCTGATCTCACTCTCGAGATTGCTTCTAGGATGCAGAATGTTATCTGCGTCGAGACTATGATGCATCTCACTTGTACCAACATGCCGATTGAGAAGATTGATCACGCGCTTGAGACGATTAGATCTAATGGAATTCAGAATGTGCTTGCTCTTAGAGGAGATCCTCCTCATGGACAAGATAAGTTTGTTCAGGTTGAAGGAGGATTTGCTTGTGCTTTGGATCTGGTGAATCACATTCGTAGCAAGTATGGTGATTACTTTGGAATCACTGTTGCTGGTTATCCTGAGGCTCATCCGGATGTTATTGAAGCTGATGGACTTGCTACTCCTGAATCTTATCAGAGTGATCTTGCTTACTTGAAGAAAAAGGTTGATGCTGGAGCAGATTTGATTGTGACTCAGCTTTTCTATGATACTGATATATTCCTCAAGTTTGTGAATGATTGTCGGCAAATCGGGATTAATTGTCCCATTGTTCCTGGAATTATGCCTATTTCCAACTACAAGGGGTTCTTGCGTATGGCTGGTTTCTGTAAGACCAAGATACCCGCTGAGCTCACTGCTGCCTTAGAGCCTATTAAGGATAATGACGAGGCTGTTAAAGCCTATGGAATTCACTTTGCAACAGAAATGTGCAAAAAGATTTTGGCCCATGGAATCACTTCCCTTCATCTCTACACATTGAACGTGGACAAATCAGCTATTGGGATATTAATGAACCTTGGTCTGATTGATGAGTCAAAAATTTCTCGTTCTCTACCTTGGAGACGCCCTGCAAATGTTTTCCGTACTAAGGAAGATGTTCGCCCAATTTTCTGGGCAAACCGTCCAAAGAGCTACATATCTAGAACAAAGGGCTGGAATGACTTCCCACATGGACGTTGGGGTGATTCACACAGTGCAGCATACAGTACACTTTCGGATTATCAGTTTGCGCGCCCAAAAGGACGTGACAAGAAGCTTCAGCAAGAATGGGTCGTCCCACTGAAAAGCATTGAAGATGTTCAAGAGGTAGGCTTTACTTTACGGACACTCGTACAAATTGTTTCGATCTCTTTCCCTCATACTCACTTGCACATTTTCATGTAA

Protein Analysis

421

Amino Acids

47.26

Weight (kDa)

6.36

Isoelectric Point (pI)

35.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTHFR PF02219 6 - 301 8.5e-125 Methylenetetrahydrofolate reductase
MTHFR_C PF21895 310 - 397 9.5e-24 MTHFR, SAM-binding regulatory domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011051)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44160 AT3G59970 AT3G59970
fragaria_vesca FvH4_1g00040 FvH4_1g00040
malus_domestica MD00G1145100.v1.1 MD02G1001200.v1.1 MD15G1141700.v1.1
prunus_persica Prupe.7G271500_v2.0.a1
pyrus_communis pycom15g12800
rosa_chinensis RchiOBHm_Chr2g0084261
rosa_laevigata RLG00000015604
rosa_multiflora Rmu_sc0003129.1_g000016
rosa_roxburghii Rroxscaffold_2G00156200
rosa_rugosa Rorug01G0452200
rosa_samantha Rh2AG000600 Rh2BG000500 Rh2CG000400 Rh2DG000600
rosa_wichuraiana Rw2G000040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 284, 1114
AccIII TCCGGA 1 cut(s) 472
AciI CCGC 1 cut(s) 726
AclWI GGATC 5 cut(s) 123, 165, 178, 332, 405
AcsI RAATTY 3 cut(s) 309, 789, 921
AcuI CTGAAG 1 cut(s) 1124
AcyI GRCGYC 2 cut(s) 90, 946
AdeI CACNNNGTG 1 cut(s) 1076
AfaI GTAC 4 cut(s) 253, 967, 1090, 1215
AfiI CCNNNNNNNGG 2 cut(s) 347, 970
AgsI TTSAA 7 cut(s) 107, 374, 485, 538, 862, 1175, 1184
AjiI CACGTC 1 cut(s) 1127
AjnI CCWGG 1 cut(s) 655
AluBI AGCT 8 cut(s) 50, 179, 488, 580, 733, 878, 1014, 1138
AluI AGCT 8 cut(s) 50, 179, 488, 580, 733, 878, 1014, 1138
Alw21I GWGCWC 1 cut(s) 735
Alw26I GTCTC 4 cut(s) 130, 224, 284, 937
AlwI GGATC 5 cut(s) 123, 165, 178, 332, 405
Ama87I CYCGRG 1 cut(s) 191
Aor13HI TCCGGA 1 cut(s) 472
AoxI GGCC 2 cut(s) 40, 825
ApeKI GCWGC 2 cut(s) 740, 1079
ApoI RAATTY 3 cut(s) 309, 789, 921
AseI ATTAAT 2 cut(s) 639, 890
Asp700I GAANNNNTTC 4 cut(s) 838, 978, 1043, 1179
AspLEI GCGC 3 cut(s) 286, 1114, 1116
AspS9I GGNCC 2 cut(s) 134, 826
AsuHPI GGTGA 3 cut(s) 415, 440, 1076
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 1 cut(s) 134
AxyI CCTNAGG 1 cut(s) 462
BamHI GGATCC 1 cut(s) 170
BanII GRGCYC 1 cut(s) 735
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 735
BbvI GCAGC 2 cut(s) 727, 1091
BccI CCATC 2 cut(s) 80, 485
BciT130I CCWGG 1 cut(s) 657
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 4 cut(s) 130, 224, 284, 937
BfaI CTAG 2 cut(s) 204, 1023
BglII AGATCT 1 cut(s) 299
BisI GCNGC 2 cut(s) 741, 1080
BlpI GCTNAGC 1 cut(s) 729
BlsI GCNGC 2 cut(s) 742, 1081
Bme1390I CCNGG 1 cut(s) 657
Bme18I GGWCC 1 cut(s) 134
BmeT110I CYCGRG 1 cut(s) 191
BmgBI CACGTC 1 cut(s) 1127
BmgT120I GGNCC 2 cut(s) 134, 826
BmiI GGNNCC 1 cut(s) 172
BmrFI CCNGG 1 cut(s) 657
BmsI GCATC 4 cut(s) 198, 228, 250, 541
BpiI GAAGAC 1 cut(s) 83
BpmI CTGGAG 1 cut(s) 576
Bpu1102I GCTNAGC 1 cut(s) 729
BpuEI CTTGAG 2 cut(s) 308, 590
Bsa29I ATCGAT 1 cut(s) 118
BsaHI GRCGYC 2 cut(s) 90, 946
BsaI GGTCTC 1 cut(s) 130
BsaJI CCNNGG 3 cut(s) 829, 898, 938
BsaWI WCCGGW 1 cut(s) 472
Bsc4I CCNNNNNNNGG 2 cut(s) 347, 970
Bse21I CCTNAGG 1 cut(s) 462
BseAI TCCGGA 1 cut(s) 472
BseBI CCWGG 1 cut(s) 657
BseCI ATCGAT 1 cut(s) 118
BseDI CCNNGG 3 cut(s) 829, 898, 938
BseGI GGATG 3 cut(s) 213, 469, 481
BseLI CCNNNNNNNGG 2 cut(s) 347, 970
BseMII CTCAG 3 cut(s) 453, 590, 720
BsePI GCGCGC 1 cut(s) 1112
BseRI GAGGAG 2 cut(s) 333, 348
BseXI GCAGC 2 cut(s) 727, 1091
BsgI GTGCAG 1 cut(s) 1098
Bsh1236I CGCG 2 cut(s) 284, 1114
BshFI GGCC 2 cut(s) 42, 827
BshVI ATCGAT 1 cut(s) 118
BsiHKAI GWGCWC 1 cut(s) 735
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 1 cut(s) 473
BslFI GGGAC 2 cut(s) 630, 1142
BslI CCNNNNNNNGG 2 cut(s) 347, 970
BsmAI GTCTC 4 cut(s) 130, 224, 284, 937
BsmBI CGTCTC 2 cut(s) 284, 937
BsmFI GGGAC 2 cut(s) 630, 1142
BsnI GGCC 2 cut(s) 42, 827
Bso31I GGTCTC 1 cut(s) 130
BsoBI CYCGRG 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 735
Bsp13I TCCGGA 1 cut(s) 472
Bsp1720I GCTNAGC 1 cut(s) 729
Bsp19I CCATGG 1 cut(s) 829
BspACI CCGC 1 cut(s) 726
BspANI GGCC 2 cut(s) 42, 827
BspCNI CTCAG 3 cut(s) 454, 589, 721
BspDI ATCGAT 1 cut(s) 118
BspEI TCCGGA 1 cut(s) 472
BspFNI CGCG 2 cut(s) 284, 1114
BspLI GGNNCC 1 cut(s) 172
BspPI GGATC 5 cut(s) 123, 165, 178, 332, 405
BspTNI GGTCTC 1 cut(s) 130
BssECI CCNNGG 3 cut(s) 829, 898, 938
BssHII GCGCGC 1 cut(s) 1112
BssNI GRCGYC 2 cut(s) 90, 946
BssT1I CCWWGG 3 cut(s) 829, 898, 938
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 4 cut(s) 448, 1004, 1076, 1088
BstACI GRCGYC 2 cut(s) 90, 946
BstC8I GCNNGC 2 cut(s) 324, 1114
BstDEI CTNAG 6 cut(s) 329, 462, 576, 729, 745, 969
BstDSI CCRYGG 1 cut(s) 829
BstF5I GGATG 3 cut(s) 213, 469, 481
BstFNI CGCG 2 cut(s) 284, 1114
BstHHI GCGC 3 cut(s) 286, 1114, 1116
BstMAI GTCTC 4 cut(s) 130, 224, 284, 937
BstMWI GCNNNNNNNGC 1 cut(s) 779
BstNI CCWGG 1 cut(s) 657
BstNSI RCATGY 1 cut(s) 262
BstSCI CCNGG 1 cut(s) 655
BstUI CGCG 2 cut(s) 284, 1114
BstV1I GCAGC 2 cut(s) 727, 1091
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 4 cut(s) 170, 299, 337, 397
BstXI CCANNNNNNTGG 1 cut(s) 993
BstYI RGATCY 4 cut(s) 170, 299, 337, 397
Bsu15I ATCGAT 1 cut(s) 118
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 2 cut(s) 42, 827
BsuTUI ATCGAT 1 cut(s) 118
BtgI CCRYGG 1 cut(s) 829
BtrI CACGTC 1 cut(s) 1127
BtsCI GGATG 3 cut(s) 213, 469, 481
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 4 cut(s) 444, 735, 1081, 1160
Cac8I GCNNGC 2 cut(s) 324, 1114
CfoI GCGC 3 cut(s) 286, 1114, 1116
Cfr13I GGNCC 2 cut(s) 134, 826
ClaI ATCGAT 1 cut(s) 118
CseI GACGC 3 cut(s) 79, 214, 954
Csp6I GTAC 4 cut(s) 252, 966, 1089, 1214
CviAII CATG 5 cut(s) 259, 347, 830, 1052, 1261
CviQI GTAC 4 cut(s) 252, 966, 1089, 1214
DdeI CTNAG 6 cut(s) 329, 462, 576, 729, 745, 969
DraIII CACNNNGTG 1 cut(s) 1076
Ecl136II GAGCTC 1 cut(s) 733
Eco130I CCWWGG 3 cut(s) 829, 898, 938
Eco24I GRGCYC 1 cut(s) 735
Eco31I GGTCTC 1 cut(s) 130
Eco32I GATATC 1 cut(s) 151
Eco47I GGWCC 1 cut(s) 134
Eco53kI GAGCTC 1 cut(s) 733
Eco57I CTGAAG 1 cut(s) 1124
Eco81I CCTNAGG 1 cut(s) 462
Eco88I CYCGRG 1 cut(s) 191
EcoICRI GAGCTC 1 cut(s) 733
EcoRI GAATTC 2 cut(s) 309, 789
EcoRII CCWGG 1 cut(s) 655
EcoRV GATATC 1 cut(s) 151
EcoT14I CCWWGG 3 cut(s) 829, 898, 938
EcoT22I ATGCAT 1 cut(s) 243
EcoT38I GRGCYC 1 cut(s) 735
ErhI CCWWGG 3 cut(s) 829, 898, 938
Esp3I CGTCTC 2 cut(s) 284, 937
FaeI CATG 5 cut(s) 262, 350, 833, 1055, 1264
FalI AAGNNNNNCTT 2 cut(s) 674, 706
FaqI GGGAC 2 cut(s) 630, 1142
FatI CATG 5 cut(s) 258, 346, 829, 1051, 1260
FauI CCCGC 1 cut(s) 733
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 2 cut(s) 741, 1080
FokI GGATG 3 cut(s) 220, 456, 488
FriOI GRGCYC 1 cut(s) 735
Fsp4HI GCNGC 2 cut(s) 741, 1080
FspBI CTAG 2 cut(s) 204, 1023
GlaI GCGC 3 cut(s) 285, 1113, 1115
GluI GCNGC 2 cut(s) 741, 1080
GsuI CTGGAG 1 cut(s) 576
HaeIII GGCC 2 cut(s) 42, 827
HapII CCGG 1 cut(s) 473
HgaI GACGC 3 cut(s) 79, 214, 954
HhaI GCGC 3 cut(s) 286, 1114, 1116
Hin1I GRCGYC 2 cut(s) 90, 946
Hin1II CATG 5 cut(s) 262, 350, 833, 1055, 1264
Hin6I GCGC 3 cut(s) 284, 1112, 1114
HinP1I GCGC 3 cut(s) 284, 1112, 1114
HindIII AAGCTT 1 cut(s) 1136
HinfI GANTC 8 cut(s) 97, 406, 441, 509, 574, 834, 914, 1067
HpaII CCGG 1 cut(s) 473
HphI GGTGA 3 cut(s) 415, 440, 1076
Hpy166II GTNNAC 2 cut(s) 868, 1091
Hpy188I TCNGA 4 cut(s) 315, 519, 906, 1099
Hpy8I GTNNAC 2 cut(s) 868, 1091
Hpy99I CGWCG 2 cut(s) 95, 230
HpyAV CCTTC 4 cut(s) 67, 151, 368, 854
HpyCH4III ACNGT 4 cut(s) 448, 1004, 1076, 1088
HpyCH4IV ACGT 3 cut(s) 864, 1057, 1126
HpyCH4V TGCA 7 cut(s) 211, 241, 800, 813, 953, 1079, 1252
HpyF10VI GCNNNNNNNGC 1 cut(s) 779
HpyF3I CTNAG 6 cut(s) 329, 462, 576, 729, 745, 969
HpySE526I ACGT 3 cut(s) 864, 1057, 1126
Hsp92I GRCGYC 2 cut(s) 90, 946
Hsp92II CATG 5 cut(s) 262, 350, 833, 1055, 1264
HspAI GCGC 3 cut(s) 284, 1112, 1114
Kpn2I TCCGGA 1 cut(s) 472
Ksp22I TGATCA 1 cut(s) 277
LmnI GCTCC 2 cut(s) 34, 557
Lsp1109I GCAGC 2 cut(s) 727, 1091
LweI GCATC 4 cut(s) 198, 228, 250, 541
MaeI CTAG 2 cut(s) 204, 1023
MaeII ACGT 3 cut(s) 864, 1057, 1126
MaeIII GTNAC 3 cut(s) 28, 571, 1127
MboII GAAGA 6 cut(s) 88, 95, 283, 550, 986, 1187
MflI RGATCY 4 cut(s) 170, 299, 337, 397
MhlI GDGCHC 1 cut(s) 735
MluCI AATT 7 cut(s) 309, 640, 660, 789, 921, 987, 1218
MlyI GAGTC 2 cut(s) 568, 923
MmeI TCCRAC 1 cut(s) 699
MnlI CCTC 9 cut(s) 326, 351, 354, 371, 457, 614, 763, 1180, 1248
Mph1103I ATGCAT 1 cut(s) 243
MroI TCCGGA 1 cut(s) 472
MroXI GAANNNNTTC 4 cut(s) 838, 978, 1043, 1179
MseI TTAA 4 cut(s) 639, 756, 777, 890
MslI CAYNNNNRTG 2 cut(s) 474, 1259
MspA1I CMGCKG 2 cut(s) 179, 728
MspI CCGG 1 cut(s) 473
MspR9I CCNGG 1 cut(s) 657
MvaI CCWGG 1 cut(s) 657
MvnI CGCG 2 cut(s) 284, 1114
MwoI GCNNNNNNNGC 1 cut(s) 779
NcoI CCATGG 1 cut(s) 829
NlaIII CATG 5 cut(s) 262, 350, 833, 1055, 1264
NlaIV GGNNCC 1 cut(s) 172
NmuCI GTSAC 3 cut(s) 28, 571, 1127
NsiI ATGCAT 1 cut(s) 243
NspI RCATGY 1 cut(s) 262
PaeR7I CTCGAG 1 cut(s) 191
PauI GCGCGC 1 cut(s) 1112
PcsI WCGNNNNNNNCGW 1 cut(s) 115
PdmI GAANNNNTTC 4 cut(s) 838, 978, 1043, 1179
PfeI GAWTC 6 cut(s) 97, 406, 441, 509, 834, 1067
PfoI TCCNGGA 1 cut(s) 655
PkrI GCNGC 2 cut(s) 742, 1081
PleI GAGTC 2 cut(s) 568, 922
PpsI GAGTC 2 cut(s) 568, 922
PshBI ATTAAT 2 cut(s) 639, 890
Psp124BI GAGCTC 1 cut(s) 735
Psp6I CCWGG 1 cut(s) 655
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 1 cut(s) 172
PspPI GGNCC 2 cut(s) 134, 826
PsuI RGATCY 4 cut(s) 170, 299, 337, 397
PteI GCGCGC 1 cut(s) 1112
PvuII CAGCTG 1 cut(s) 179
RsaI GTAC 4 cut(s) 253, 967, 1090, 1215
RsaNI GTAC 4 cut(s) 252, 966, 1089, 1214
RseI CAYNNNNRTG 2 cut(s) 474, 1259
SacI GAGCTC 1 cut(s) 735
SaqAI TTAA 4 cut(s) 639, 756, 777, 890
SatI GCNGC 2 cut(s) 741, 1080
Sau96I GGNCC 2 cut(s) 134, 826
SchI GAGTC 2 cut(s) 568, 923
ScrFI CCNGG 1 cut(s) 657
SduI GDGCHC 1 cut(s) 735
SfaNI GCATC 4 cut(s) 198, 228, 250, 541
Sfr274I CTCGAG 1 cut(s) 191
SinI GGWCC 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 191
SmiMI CAYNNNNRTG 2 cut(s) 474, 1259
SmlI CTYRAG 3 cut(s) 191, 287, 605
SmoI CTYRAG 3 cut(s) 191, 287, 605
Sse9I AATT 7 cut(s) 309, 640, 660, 789, 921, 987, 1218
SsiI CCGC 1 cut(s) 726
SspMI CTAG 2 cut(s) 204, 1023
SstI GAGCTC 1 cut(s) 735
StyD4I CCNGG 1 cut(s) 655
StyI CCWWGG 3 cut(s) 829, 898, 938
TaaI ACNGT 4 cut(s) 448, 1004, 1076, 1088
TaiI ACGT 3 cut(s) 867, 1060, 1129
TaqI TCGA 6 cut(s) 60, 93, 118, 192, 228, 1226
TasI AATT 7 cut(s) 309, 640, 660, 789, 921, 987, 1218
TatI WGTACW 1 cut(s) 1088
TfiI GAWTC 6 cut(s) 97, 406, 441, 509, 834, 1067
Tru1I TTAA 4 cut(s) 639, 756, 777, 890
Tru9I TTAA 4 cut(s) 639, 756, 777, 890
TscAI CASTG 4 cut(s) 451, 742, 1081, 1167
TseFI GTSAC 3 cut(s) 28, 571, 1127
TseI GCWGC 2 cut(s) 740, 1079
Tsp45I GTSAC 3 cut(s) 28, 571, 1127
TspDTI ATGAA 4 cut(s) 17, 836, 908, 1249
TspGWI ACGGA 4 cut(s) 16, 47, 953, 1219
TspRI CASTG 4 cut(s) 451, 742, 1081, 1167
VpaK11BI GGWCC 1 cut(s) 134
VspI ATTAAT 2 cut(s) 639, 890
XapI RAATTY 3 cut(s) 309, 789, 921
XbaI TCTAGA 1 cut(s) 1022
XceI RCATGY 1 cut(s) 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 1056
XhoI CTCGAG 1 cut(s) 191
XmnI GAANNNNTTC 4 cut(s) 838, 978, 1043, 1179
XspI CTAG 2 cut(s) 204, 1023
Zsp2I ATGCAT 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.