AT2G44500

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005122
Physical Location & Seq
Forward (+)
18374162 .. 18376799
2638 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44500.2

Sequence Viewer

Length: 1722 bp
ATGGCGTTATCGAAAAACAGTAACAGTAACAGCTTTAACAAGAAGAAAGTTTCGTATATCTCAGTCCCTTCTCAGATCATAAACTCTTTATCATCTTCCTCATTACAATCTCTTCTTGTTTCTCCCAAGAAATCATCAAGAAGCACCAACAGATTCAGCTTCTCTTATAGGAACCCTAGAATCTGGTTCTTTACTCTCTTCCTCGTCTCACTCTTTGGAATGTTGAAACTCGGTTTCAACGTTGACCCAATCTCTCTCCCTTTCTCACGTTACCCTTGTTCAACGACCCAACAACCACTAAGCTTCGACGGAGAACAAAACGCTGCATCCCATCTTGGTTTGGCTCAAGAGCCGATTTTGTCTACTGGGTCATCGAATTCGAATGCGATTATACAATTAAACGGTGGAAAGAACGAGACTTTGTTAACGGAAGGTGATTTCTGGAAGCAACCTGATGGGTTAGGGTTTAAGCCCTGTTTGGGATTTACAAGTCAATATAGAAAAGATAGCAATTCGATTTTGAAGAACAGATGGAAGTATTTGCTTGTTGTTGTCTCTGGTGGGATGAATCAGCAAAGGAATCAGATTGTTGATGCTGTTGTGATCGCTAGGATCCTTGGAGCTTCTTTAGTTGTTCCTGTTTTGCAGGTTAATGTCATTTGGGGAGACGAAAGTGAATTTGCGGATATATTCGATTTGGAGCATTTCAAGGATGTTTTAGCAGATGATGTTCATATAGTTTCGTCTTTACCTTCTACACATGTTATGACGAGACCTGTTGAAGAGAAGAGGACTCCACTTCATGCTTCTCCTCAATGGATTCGTGCTCATTATCTTAAGCGAATTAACAGAGAAAGAGTTTTACTTCTCCGTGGACTCGATTCAAGACTCTCCAAAGACCTCCCTTCAGATCTTCAGAAACTCCGATGCAAGGTTGCTTTCCAAGCGCTTAGATTTTCTCCGAGGATTCTTGAACTCGGTAATAAACTAGCTTCAAGAATGCGTAACCAAGGACAGTATCTCTCACTTCATCTAAGAATGGAGAAAGATGTTTGGGTTAGAACTGGTTGTCTCCCTGGTTTAACTCCCGAGTACGATGAAATAGTCAACAGCGAAAGAGAACGACACCCTGAGCTTCTTACCGGTAGATCAAACATGACTTATCACGAGAGAAAACTTGCCGGTTTATGCCCTTTAACTGCTCTAGAAGTCACAAGGCTGCTTAAAGCATTAGAAGCTCCAAAGGATGCGAGAATCTATTGGGCAGGAGGAGAGCCTCTAGGAGGAAAAGAGGTTTTGGAGCCACTAACTAAAGAATTCCCTCAGTTCTACAACAAGCACGATCTTGCTTTACCTGGCGAGCTTGAACCATTTGCCAACAAAGCTTCGGTTATGGCTGCTATAGACTACATTGTCTGCGAAAAGAGCGATGTTTTCATACCGTCTCATGGAGGAAATATGGGACACGCGTTACAAGGGCAAAGAGCTTACGCTGGTCACAAGAAATACATCACTCCTAATAAGAGACAGATGCTTCCTTATTTCATGAACTCGTCGCTTCCTGAATCGGATTTTAACAGAATTGTGAAAGATCTTCATCGAGAATCGCTAGGACAGCCGGAGTTGAGAATGAGCAAAGCTGGTAAAGATGTTACAAAACATCCTGTTCCAGAGTGTATGTGCTCGGATAGACAACAACAAGAACAACAGTCCGATGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

573

Amino Acids

64.58

Weight (kDa)

9.26

Isoelectric Point (pI)

48.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 180 - 404 6e-60 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1412
Acc36I ACCTGC 1 cut(s) 637
AccI GTMKAC 1 cut(s) 362
AccII CGCG 1 cut(s) 1469
AciI CCGC 1 cut(s) 683
AclI AACGTT 1 cut(s) 240
AclWI GGATC 2 cut(s) 607, 620
AcsI RAATTY 3 cut(s) 376, 677, 1316
AcuI CTGAAG 2 cut(s) 891, 899
AfaI GTAC 1 cut(s) 1094
AfeI AGCGCT 1 cut(s) 948
AfiI CCNNNNNNNGG 4 cut(s) 479, 931, 1283, 1448
AflII CTTAAG 1 cut(s) 836
AflIII ACRYGT 2 cut(s) 760, 1467
AgeI ACCGGT 1 cut(s) 1142
AjnI CCWGG 2 cut(s) 1075, 1354
Alw21I GWGCWC 2 cut(s) 829, 1685
Alw26I GTCTC 8 cut(s) 211, 410, 559, 660, 766, 1076, 1449, 1519
AlwI GGATC 2 cut(s) 607, 620
Ama87I CYCGRG 1 cut(s) 1088
Aor51HI AGCGCT 1 cut(s) 948
ApeKI GCWGC 3 cut(s) 323, 1219, 1397
ApoI RAATTY 3 cut(s) 376, 677, 1316
AsiGI ACCGGT 1 cut(s) 1142
AspLEI GCGC 1 cut(s) 949
AsuHPI GGTGA 1 cut(s) 446
AsuII TTCGAA 1 cut(s) 380
AvaI CYCGRG 1 cut(s) 1088
BamHI GGATCC 1 cut(s) 612
BauI CACGAG 1 cut(s) 1166
Bbv12I GWGCWC 2 cut(s) 829, 1685
BbvI GCAGC 3 cut(s) 310, 1206, 1384
BccI CCATC 3 cut(s) 339, 449, 525
BciT130I CCWGG 2 cut(s) 1077, 1356
BcoDI GTCTC 8 cut(s) 211, 410, 559, 660, 766, 1076, 1449, 1519
BfaI CTAG 6 cut(s) 177, 609, 989, 1205, 1280, 1610
BfmI CTRYAG 1 cut(s) 1401
BfoI RGCGCY 1 cut(s) 950
BfrI CTTAAG 1 cut(s) 836
BfuAI ACCTGC 1 cut(s) 637
BglII AGATCT 2 cut(s) 910, 1591
BisI GCNGC 3 cut(s) 324, 1220, 1398
BlsI GCNGC 3 cut(s) 325, 1221, 1399
Bme1390I CCNGG 2 cut(s) 1077, 1356
BmeT110I CYCGRG 1 cut(s) 1088
BmiI GGNNCC 3 cut(s) 173, 614, 1302
BmrFI CCNGG 2 cut(s) 1077, 1356
BmrI ACTGGG 1 cut(s) 375
BmsI GCATC 6 cut(s) 335, 583, 917, 1237, 1521, 1705
BmuI ACTGGG 1 cut(s) 375
Bpu10I CCTNAGC 1 cut(s) 1131
Bpu14I TTCGAA 1 cut(s) 380
BpuEI CTTGAG 1 cut(s) 330
BsaBI GATNNNNATC 1 cut(s) 1596
BsaI GGTCTC 1 cut(s) 766
BsaJI CCNNGG 5 cut(s) 616, 871, 962, 1009, 1075
BsaWI WCCGGW 1 cut(s) 1142
Bsc4I CCNNNNNNNGG 4 cut(s) 479, 931, 1283, 1448
Bse118I RCCGGY 2 cut(s) 1142, 1181
Bse1I ACTGG 2 cut(s) 370, 1069
Bse8I GATNNNNATC 1 cut(s) 1596
BseBI CCWGG 2 cut(s) 1077, 1356
BseDI CCNNGG 5 cut(s) 616, 871, 962, 1009, 1075
BseGI GGATG 5 cut(s) 326, 570, 718, 1252, 1660
BseJI GATNNNNATC 1 cut(s) 1596
BseLI CCNNNNNNNGG 4 cut(s) 479, 931, 1283, 1448
BseMII CTCAG 4 cut(s) 75, 86, 1122, 1337
BseNI ACTGG 2 cut(s) 370, 1069
BseRI GAGGAG 2 cut(s) 801, 1284
BseXI GCAGC 3 cut(s) 310, 1206, 1384
Bsh1236I CGCG 1 cut(s) 1469
BshTI ACCGGT 1 cut(s) 1142
BsiHKAI GWGCWC 2 cut(s) 829, 1685
BsiHKCI CYCGRG 1 cut(s) 1088
BsiSI CCGG 3 cut(s) 1143, 1182, 1619
BslFI GGGAC 2 cut(s) 50, 1476
BslI CCNNNNNNNGG 4 cut(s) 479, 931, 1283, 1448
BsmAI GTCTC 8 cut(s) 211, 410, 559, 660, 766, 1076, 1449, 1519
BsmBI CGTCTC 3 cut(s) 211, 660, 1449
BsmFI GGGAC 2 cut(s) 50, 1476
BsmI GAATGC 2 cut(s) 388, 1005
Bso31I GGTCTC 1 cut(s) 766
BsoBI CYCGRG 1 cut(s) 1088
Bsp119I TTCGAA 1 cut(s) 380
Bsp1286I GDGCHC 2 cut(s) 829, 1685
Bsp143I GATC 7 cut(s) 75, 603, 612, 910, 1148, 1342, 1591
BspACI CCGC 1 cut(s) 683
BspCNI CTCAG 4 cut(s) 74, 85, 1123, 1336
BspFNI CGCG 1 cut(s) 1469
BspHI TCATGA 1 cut(s) 1545
BspLI GGNNCC 3 cut(s) 173, 614, 1302
BspMI ACCTGC 1 cut(s) 637
BspPI GGATC 2 cut(s) 607, 620
BspT104I TTCGAA 1 cut(s) 380
BspTI CTTAAG 1 cut(s) 836
BspTNI GGTCTC 1 cut(s) 766
BsrFI RCCGGY 2 cut(s) 1142, 1181
BsrI ACTGG 2 cut(s) 370, 1069
BssAI RCCGGY 2 cut(s) 1142, 1181
BssECI CCNNGG 5 cut(s) 616, 871, 962, 1009, 1075
BssMI GATC 7 cut(s) 75, 603, 612, 910, 1148, 1342, 1591
BssSI CACGAG 1 cut(s) 1166
BssT1I CCWWGG 2 cut(s) 616, 1009
Bst2BI CACGAG 1 cut(s) 1166
Bst2UI CCWGG 2 cut(s) 1077, 1356
Bst4CI ACNGT 6 cut(s) 20, 26, 404, 1017, 1443, 1710
Bst6I CTCTTC 4 cut(s) 117, 203, 777, 782
BstAFI CTTAAG 1 cut(s) 836
BstBI TTCGAA 1 cut(s) 380
BstC8I GCNNGC 1 cut(s) 1361
BstDEI CTNAG 7 cut(s) 61, 72, 299, 950, 1034, 1131, 1323
BstDSI CCRYGG 1 cut(s) 871
BstENI CCTNNNNNAGG 1 cut(s) 1281
BstF5I GGATG 5 cut(s) 326, 570, 718, 1252, 1660
BstFNI CGCG 1 cut(s) 1469
BstH2I RGCGCY 1 cut(s) 950
BstHHI GCGC 1 cut(s) 949
BstKTI GATC 7 cut(s) 78, 606, 615, 913, 1151, 1345, 1594
BstMAI GTCTC 8 cut(s) 211, 410, 559, 660, 766, 1076, 1449, 1519
BstMBI GATC 7 cut(s) 75, 603, 612, 910, 1148, 1342, 1591
BstMWI GCNNNNNNNGC 5 cut(s) 944, 1235, 1382, 1425, 1615
BstNI CCWGG 2 cut(s) 1077, 1356
BstNSI RCATGY 1 cut(s) 764
BstSCI CCNGG 2 cut(s) 1075, 1354
BstSFI CTRYAG 1 cut(s) 1401
BstUI CGCG 1 cut(s) 1469
BstV1I GCAGC 3 cut(s) 310, 1206, 1384
BstX2I RGATCY 3 cut(s) 612, 910, 1591
BstYI RGATCY 3 cut(s) 612, 910, 1591
BtgI CCRYGG 1 cut(s) 871
BtgZI GCGATG 1 cut(s) 1443
BtsCI GGATG 5 cut(s) 326, 570, 718, 1252, 1660
BveI ACCTGC 1 cut(s) 637
Cac8I GCNNGC 1 cut(s) 1361
CciI TCATGA 1 cut(s) 1545
CfoI GCGC 1 cut(s) 949
Cfr10I RCCGGY 2 cut(s) 1142, 1181
Csp6I GTAC 1 cut(s) 1093
CspAI ACCGGT 1 cut(s) 1142
CviAII CATG 5 cut(s) 761, 803, 1156, 1448, 1546
CviQI GTAC 1 cut(s) 1093
DdeI CTNAG 7 cut(s) 61, 72, 299, 950, 1034, 1131, 1323
DpnI GATC 7 cut(s) 77, 605, 614, 912, 1150, 1344, 1593
DpnII GATC 7 cut(s) 75, 603, 612, 910, 1148, 1342, 1591
DrdI GACNNNNNNGTC 1 cut(s) 1412
DseDI GACNNNNNNGTC 1 cut(s) 1412
Eam1104I CTCTTC 4 cut(s) 117, 203, 777, 782
EarI CTCTTC 4 cut(s) 117, 203, 777, 782
Eco130I CCWWGG 2 cut(s) 616, 1009
Eco31I GGTCTC 1 cut(s) 766
Eco47III AGCGCT 1 cut(s) 948
Eco57I CTGAAG 2 cut(s) 891, 899
Eco88I CYCGRG 1 cut(s) 1088
EcoNI CCTNNNNNAGG 1 cut(s) 1281
EcoRI GAATTC 2 cut(s) 376, 1316
EcoRII CCWGG 2 cut(s) 1075, 1354
EcoT14I CCWWGG 2 cut(s) 616, 1009
ErhI CCWWGG 2 cut(s) 616, 1009
Esp3I CGTCTC 3 cut(s) 211, 660, 1449
FaeI CATG 5 cut(s) 764, 806, 1159, 1451, 1549
FaqI GGGAC 2 cut(s) 50, 1476
FatI CATG 5 cut(s) 760, 802, 1155, 1447, 1545
FblI GTMKAC 1 cut(s) 362
Fnu4HI GCNGC 3 cut(s) 324, 1220, 1398
FokI GGATG 5 cut(s) 313, 577, 725, 1259, 1647
Fsp4HI GCNGC 3 cut(s) 324, 1220, 1398
FspBI CTAG 6 cut(s) 177, 609, 989, 1205, 1280, 1610
GlaI GCGC 1 cut(s) 948
GluI GCNGC 3 cut(s) 324, 1220, 1398
HaeII RGCGCY 1 cut(s) 950
HapII CCGG 3 cut(s) 1143, 1182, 1619
HhaI GCGC 1 cut(s) 949
Hin1II CATG 5 cut(s) 764, 806, 1159, 1451, 1549
Hin6I GCGC 1 cut(s) 947
HinP1I GCGC 1 cut(s) 947
HincII GTYRAC 3 cut(s) 244, 426, 1108
HindII GTYRAC 3 cut(s) 244, 426, 1108
HindIII AAGCTT 2 cut(s) 301, 1383
HpaI GTTAAC 1 cut(s) 426
HpaII CCGG 3 cut(s) 1143, 1182, 1619
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 5 cut(s) 244, 363, 426, 875, 1108
Hpy188I TCNGA 9 cut(s) 75, 585, 910, 918, 926, 963, 1570, 1687, 1714
Hpy8I GTNNAC 5 cut(s) 244, 363, 426, 875, 1108
Hpy99I CGWCG 2 cut(s) 311, 1558
HpyAV CCTTC 4 cut(s) 78, 425, 762, 915
HpyCH4III ACNGT 6 cut(s) 20, 26, 404, 1017, 1443, 1710
HpyCH4IV ACGT 2 cut(s) 240, 268
HpyCH4V TGCA 3 cut(s) 326, 646, 930
HpyF10VI GCNNNNNNNGC 5 cut(s) 944, 1235, 1382, 1425, 1615
HpyF3I CTNAG 7 cut(s) 61, 72, 299, 950, 1034, 1131, 1323
HpySE526I ACGT 2 cut(s) 240, 268
Hsp92II CATG 5 cut(s) 764, 806, 1159, 1451, 1549
HspAI GCGC 1 cut(s) 947
KspAI GTTAAC 1 cut(s) 426
Kzo9I GATC 7 cut(s) 75, 603, 612, 910, 1148, 1342, 1591
LmnI GCTCC 4 cut(s) 620, 700, 1243, 1300
Lsp1109I GCAGC 3 cut(s) 310, 1206, 1384
LweI GCATC 6 cut(s) 335, 583, 917, 1237, 1521, 1705
MaeI CTAG 6 cut(s) 177, 609, 989, 1205, 1280, 1610
MaeII ACGT 2 cut(s) 240, 268
MaeIII GTNAC 8 cut(s) 20, 26, 269, 1004, 1210, 1470, 1496, 1651
MalI GATC 7 cut(s) 77, 605, 614, 912, 1150, 1344, 1593
MboI GATC 7 cut(s) 75, 603, 612, 910, 1148, 1342, 1591
MboII GAAGA 9 cut(s) 55, 87, 104, 190, 535, 794, 799, 905, 1586
MflI RGATCY 3 cut(s) 612, 910, 1591
MhlI GDGCHC 2 cut(s) 829, 1685
MluCI AATT 7 cut(s) 376, 395, 511, 677, 843, 1316, 1581
MluI ACGCGT 1 cut(s) 1467
MlyI GAGTC 3 cut(s) 787, 870, 882
MspCI CTTAAG 1 cut(s) 836
MspI CCGG 3 cut(s) 1143, 1182, 1619
MspR9I CCNGG 2 cut(s) 1077, 1356
Mva1269I GAATGC 2 cut(s) 388, 1005
MvaI CCWGG 2 cut(s) 1077, 1356
MvnI CGCG 1 cut(s) 1469
MwoI GCNNNNNNNGC 5 cut(s) 944, 1235, 1382, 1425, 1615
NdeII GATC 7 cut(s) 75, 603, 612, 910, 1148, 1342, 1591
NlaIII CATG 5 cut(s) 764, 806, 1159, 1451, 1549
NlaIV GGNNCC 3 cut(s) 173, 614, 1302
NmuCI GTSAC 2 cut(s) 1210, 1496
NspI RCATGY 1 cut(s) 764
NspV TTCGAA 1 cut(s) 380
PagI TCATGA 1 cut(s) 1545
PciI ACATGT 1 cut(s) 760
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 2 cut(s) 388, 1005
PinAI ACCGGT 1 cut(s) 1142
PkrI GCNGC 3 cut(s) 325, 1221, 1399
PleI GAGTC 3 cut(s) 787, 870, 882
PpsI GAGTC 3 cut(s) 787, 870, 882
PscI ACATGT 1 cut(s) 760
Psp1406I AACGTT 1 cut(s) 240
Psp6I CCWGG 2 cut(s) 1075, 1354
PspGI CCWGG 2 cut(s) 1075, 1354
PspN4I GGNNCC 3 cut(s) 173, 614, 1302
PsuI RGATCY 3 cut(s) 612, 910, 1591
RsaI GTAC 1 cut(s) 1094
RsaNI GTAC 1 cut(s) 1093
SatI GCNGC 3 cut(s) 324, 1220, 1398
Sau3AI GATC 7 cut(s) 75, 603, 612, 910, 1148, 1342, 1591
SchI GAGTC 3 cut(s) 787, 870, 882
ScrFI CCNGG 2 cut(s) 1077, 1356
SduI GDGCHC 2 cut(s) 829, 1685
SfaNI GCATC 6 cut(s) 335, 583, 917, 1237, 1521, 1705
SfcI CTRYAG 1 cut(s) 1401
SfuI TTCGAA 1 cut(s) 380
SmlI CTYRAG 2 cut(s) 345, 836
SmoI CTYRAG 2 cut(s) 345, 836
Sse9I AATT 7 cut(s) 376, 395, 511, 677, 843, 1316, 1581
SsiI CCGC 1 cut(s) 683
SspMI CTAG 6 cut(s) 177, 609, 989, 1205, 1280, 1610
StyD4I CCNGG 2 cut(s) 1075, 1354
StyI CCWWGG 2 cut(s) 616, 1009
TaaI ACNGT 6 cut(s) 20, 26, 404, 1017, 1443, 1710
TaiI ACGT 2 cut(s) 243, 271
TaqI TCGA 8 cut(s) 11, 306, 374, 380, 515, 693, 879, 1600
TasI AATT 7 cut(s) 376, 395, 511, 677, 843, 1316, 1581
TseFI GTSAC 2 cut(s) 1210, 1496
TseI GCWGC 3 cut(s) 323, 1219, 1397
Tsp45I GTSAC 2 cut(s) 1210, 1496
TspDTI ATGAA 9 cut(s) 581, 722, 791, 1019, 1113, 1426, 1534, 1562, 1586
TspGWI ACGGA 3 cut(s) 324, 443, 860
Vha464I CTTAAG 1 cut(s) 836
XagI CCTNNNNNAGG 1 cut(s) 1281
XapI RAATTY 3 cut(s) 376, 677, 1316
XbaI TCTAGA 1 cut(s) 1204
XceI RCATGY 1 cut(s) 764
XmiI GTMKAC 1 cut(s) 362
XspI CTAG 6 cut(s) 177, 609, 989, 1205, 1280, 1610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.