RLG00000030698

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005122
Physical Location & Seq
Forward (+)
66100193 .. 66111740
11548 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030698

Sequence Viewer

Length: 1737 bp
ATGGCCAAGTCAAAAAGCAACAAGAACAAGGTCTCTTACATTTCTGTCCCTTCTCAGATAATCAATTCTTTGTCTGCATCTTCTCTTCAATCCCTCCTTACCTCACCCAAGAACTCATGTTCAAGGAGCAAAGCAAGGTGCTTGAGCTTGCCGACCAAGTACAGGAGCCCAATTAGTTTCTGGGTTTTCTTCCTCTTCCTCTTCGGAATTCTTGGTATGCTCAAGTTGATATTCAATTTAAACACTTTGATCCCTTATTCTCCTTACCCACGCGCCACAAGCCAGTTCCACGCCTTAGTCTCCTCAAATGGGTATTTGAAATCACACACGAGTATTGCATCAAACGAAAATGGTCAGAAAGCTGAGGTCTTTGACAGTTTCAGCCATTCGCTTCCCTTGATTCTAAAGGAGAAGGAGGAGGAGCCCAAATCAGAACCAAACAGCGACAAGGCAATAAGTGACTTCTGGAAGCAACCAGATGGTTTGGGGTATAGGCCATGCTTGGATTTCAGCCGGGACTATCAGAAGGGAAGTGTAGTGACAGTGAAGGACAGGACTAAATACCTCATGGTTGTGGTTGCTGGAGGGCTGAACCAACAAAGGAACCAGATTGTCGATGCTGTGGTTATTGCTAGGATTCTTGGGGCAGCTTTGGTCGTTCCCATTTTGCAAGTCAATGTCATCTGGGGAGATGAAAGTAAATTTTCTGATATATTTGATATGGAGCACTTCAAGAAAGTTCTTGCTAATGATGTCCGTATAGTTTCTTCACTACCATCAACACATACAATGACAAGGCCAATGGAGAAGAAACTGACTCCTTTTCATGTTTCTCCTAGTTGGATCCGGTCACGCTATCTCAAGCGGATTAAAAGAGAAGGAGTTTTGCTTCTTGGAGGCTTCAATTCAAGGCTTTATAAGGATCTTCCTTCTGATCTTCAGAAGCTTCGTTGCAAGGTTGCTTTTCATGCACTGAGGTTTGTTCCTCCAATTTTGGGAATTGGTAACAAGCTTGCGGAGAGGATGCGTAGCAAGGGACCCTACCTTGCCCTTCATCTAAGAATGGAGAAGGATGTATGGGTGAGGACTGGTTGCCTTCCTGGTCTGAGTCATGAATACGATGAGATAGTAAACAGTGAAAGGATACAACGGCCTGAGCTCCTAACTGCAAGATCAAACATGACTTACCATGAAAGAAAACTTGCTGGTCACTGCCCCTTGAACGCCGTGGAAGTGACTAGGTTGCTAAAAGCTCTAGGAGCTCCCAAAGATGCAAGAATATATTGGGCTGGAGGGGAGCCATTGGGCGGAAGAGAAGCCTTGCTACCATTAACCCGGGAGTTTTCCCATTTTTACAATAAGGAAGATCTTGCTTTGCCTGGTGAGCTAGAACCTTTTGCCAAAAGAGCTTCTTTCATGGCTGCCATTGATTACATAGTTTCCGAGAACAGTGATGTCTTCATGCCATCTCATGGAGGAAATATGGGCCATGCACTCCAGGGACAACGTGCCTATGCTGGCCACAAGAAGTATATAACACCAGACAAAAGGCGTATGCTTCCTTATTTTCTGGACTCTTCGCTCCCTGAAGTAGAGTTTAATAGGATCATAAAGGAGTTGCACAGTGATTCCTTAGGGCAGCCAGACATTCGGATTAGTAAAGTTGGAAGAGATGTTACAAAGTATCCCATCCCGGAGTGTATGTGCAGTGATTCTCATGCTCATCCCCACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

579

Amino Acids

65.2

Weight (kDa)

9.53

Isoelectric Point (pI)

43.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 188 - 505 1.4e-85 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 918
AccB7I CCANNNNNTGG 1 cut(s) 1472
AccII CGCG 1 cut(s) 273
AciI CCGC 3 cut(s) 865, 1016, 1308
AclWI GGATC 5 cut(s) 244, 838, 851, 930, 1613
AcoI YGGCCR 2 cut(s) 3, 1519
AcsI RAATTY 2 cut(s) 207, 701
AcuI CTGAAG 2 cut(s) 923, 1608
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 5 cut(s) 162, 309, 995, 1307, 1472
AgsI TTSAA 8 cut(s) 89, 123, 235, 319, 733, 904, 909, 1222
AjnI CCWGG 3 cut(s) 1099, 1378, 1497
Alw21I GWGCWC 3 cut(s) 729, 1161, 1264
Alw26I GTCTC 2 cut(s) 37, 304
AlwI GGATC 5 cut(s) 244, 838, 851, 930, 1613
Ama87I CYCGRG 1 cut(s) 1335
AoxI GGCC 6 cut(s) 3, 494, 797, 1151, 1486, 1519
ApeKI GCWGC 3 cut(s) 647, 1421, 1639
ApoI RAATTY 2 cut(s) 207, 701
AspLEI GCGC 1 cut(s) 275
AspS9I GGNCC 2 cut(s) 1037, 1486
AsuC2I CCSGG 4 cut(s) 515, 1336, 1337, 1694
AsuHPI GGTGA 3 cut(s) 96, 1093, 1394
AvaI CYCGRG 1 cut(s) 1335
AvaII GGWCC 1 cut(s) 1037
AxyI CCTNAGG 1 cut(s) 1633
BalI TGGCCA 2 cut(s) 5, 1521
BamHI GGATCC 1 cut(s) 843
BanII GRGCYC 4 cut(s) 170, 426, 1161, 1264
BarI GAAGNNNNNNTAC 6 cut(s) 751, 783, 1308, 1340, 1660, 1692
BauI CACGAG 1 cut(s) 328
BbsI GAAGAC 1 cut(s) 1450
Bbv12I GWGCWC 3 cut(s) 729, 1161, 1264
BbvCI CCTCAGC 1 cut(s) 363
BbvI GCAGC 3 cut(s) 659, 1408, 1651
BccI CCATC 4 cut(s) 473, 784, 1474, 1697
BceAI ACGGC 2 cut(s) 1166, 1211
BciT130I CCWGG 3 cut(s) 1101, 1380, 1499
BciVI GTATCC 2 cut(s) 1137, 1695
BcnI CCSGG 4 cut(s) 515, 1336, 1337, 1694
BcoDI GTCTC 2 cut(s) 37, 304
BfaI CTAG 5 cut(s) 633, 837, 1239, 1256, 1388
BfuI GTATCC 2 cut(s) 1137, 1695
BglII AGATCT 1 cut(s) 1366
BisI GCNGC 3 cut(s) 648, 1422, 1640
BlsI GCNGC 3 cut(s) 649, 1423, 1641
Bme1390I CCNGG 7 cut(s) 515, 1101, 1336, 1337, 1380, 1499, 1694
Bme18I GGWCC 1 cut(s) 1037
BmeT110I CYCGRG 1 cut(s) 1335
BmgT120I GGNCC 2 cut(s) 1037, 1486
BmiI GGNNCC 7 cut(s) 167, 423, 605, 845, 1038, 1039, 1299
BmrFI CCNGG 7 cut(s) 515, 1101, 1336, 1337, 1380, 1499, 1694
BmsI GCATC 5 cut(s) 86, 347, 607, 1014, 1261
BpiI GAAGAC 1 cut(s) 1450
BpmI CTGGAG 3 cut(s) 603, 1311, 1481
Bpu10I CCTNAGC 2 cut(s) 363, 1155
BpuEI CTTGAG 3 cut(s) 163, 206, 845
BpuMI CCSGG 4 cut(s) 515, 1336, 1337, 1694
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 3 cut(s) 1227, 1335, 1498
BsaWI WCCGGW 1 cut(s) 846
BsaXI ACNNNNNCTCC 2 cut(s) 1688, 1718
Bsc4I CCNNNNNNNGG 5 cut(s) 162, 309, 995, 1307, 1472
Bse1I ACTGG 2 cut(s) 283, 1093
Bse21I CCTNAGG 1 cut(s) 1633
BseBI CCWGG 3 cut(s) 1101, 1380, 1499
BseDI CCNNGG 3 cut(s) 1227, 1335, 1498
BseGI GGATG 4 cut(s) 1029, 1078, 1689, 1723
BseLI CCNNNNNNNGG 5 cut(s) 162, 309, 995, 1307, 1472
BseMII CTCAG 5 cut(s) 68, 354, 965, 1097, 1146
BseNI ACTGG 2 cut(s) 283, 1093
BseRI GAGGAG 3 cut(s) 292, 431, 434
BseXI GCAGC 3 cut(s) 659, 1408, 1651
BsgI GTGCAG 1 cut(s) 1726
Bsh1236I CGCG 1 cut(s) 273
BshFI GGCC 6 cut(s) 5, 496, 799, 1153, 1488, 1521
BsiHKAI GWGCWC 3 cut(s) 729, 1161, 1264
BsiHKCI CYCGRG 1 cut(s) 1335
BsiSI CCGG 4 cut(s) 514, 847, 1336, 1694
BslFI GGGAC 4 cut(s) 32, 530, 1050, 1515
BslI CCNNNNNNNGG 5 cut(s) 162, 309, 995, 1307, 1472
BsmAI GTCTC 2 cut(s) 37, 304
BsmFI GGGAC 4 cut(s) 32, 530, 1050, 1515
BsnI GGCC 6 cut(s) 5, 496, 799, 1153, 1488, 1521
Bso31I GGTCTC 1 cut(s) 37
BsoBI CYCGRG 1 cut(s) 1335
Bsp1286I GDGCHC 5 cut(s) 170, 426, 729, 1161, 1264
Bsp143I GATC 7 cut(s) 249, 843, 922, 934, 1172, 1366, 1605
BspACI CCGC 3 cut(s) 865, 1016, 1308
BspANI GGCC 6 cut(s) 5, 496, 799, 1153, 1488, 1521
BspCNI CTCAG 5 cut(s) 67, 355, 966, 1098, 1147
BspFNI CGCG 1 cut(s) 273
BspHI TCATGA 1 cut(s) 1111
BspLI GGNNCC 7 cut(s) 167, 423, 605, 845, 1038, 1039, 1299
BspPI GGATC 5 cut(s) 244, 838, 851, 930, 1613
BspTNI GGTCTC 1 cut(s) 37
BsrI ACTGG 2 cut(s) 283, 1093
BssECI CCNNGG 3 cut(s) 1227, 1335, 1498
BssMI GATC 7 cut(s) 249, 843, 922, 934, 1172, 1366, 1605
BssSI CACGAG 1 cut(s) 328
Bst2BI CACGAG 1 cut(s) 328
Bst2UI CCWGG 3 cut(s) 1101, 1380, 1499
Bst4CI ACNGT 5 cut(s) 377, 544, 1136, 1451, 1625
Bst6I CTCTTC 6 cut(s) 90, 200, 206, 1306, 1582, 1663
BstC8I GCNNGC 3 cut(s) 149, 1014, 1519
BstDEI CTNAG 8 cut(s) 54, 295, 363, 974, 1058, 1106, 1155, 1633
BstDSI CCRYGG 1 cut(s) 1227
BstF5I GGATG 4 cut(s) 1029, 1078, 1689, 1723
BstFNI CGCG 1 cut(s) 273
BstHHI GCGC 1 cut(s) 275
BstKTI GATC 7 cut(s) 252, 846, 925, 937, 1175, 1369, 1608
BstMAI GTCTC 2 cut(s) 37, 304
BstMBI GATC 7 cut(s) 249, 843, 922, 934, 1172, 1366, 1605
BstMWI GCNNNNNNNGC 5 cut(s) 279, 968, 1259, 1384, 1406
BstNI CCWGG 3 cut(s) 1101, 1380, 1499
BstSCI CCNGG 7 cut(s) 513, 1099, 1334, 1335, 1378, 1497, 1692
BstUI CGCG 1 cut(s) 273
BstV1I GCAGC 3 cut(s) 659, 1408, 1651
BstV2I GAAGAC 1 cut(s) 1450
BstX2I RGATCY 3 cut(s) 843, 922, 1366
BstYI RGATCY 3 cut(s) 843, 922, 1366
Bsu36I CCTNAGG 1 cut(s) 1633
BsuI GTATCC 2 cut(s) 1137, 1695
BsuRI GGCC 6 cut(s) 5, 496, 799, 1153, 1488, 1521
BtgI CCRYGG 1 cut(s) 1227
BtsCI GGATG 4 cut(s) 1029, 1078, 1689, 1723
BtsI GCAGTG 2 cut(s) 1210, 1714
BtsIMutI CAGTG 7 cut(s) 549, 971, 1141, 1210, 1456, 1630, 1714
Cac8I GCNNGC 3 cut(s) 149, 1014, 1519
CciI TCATGA 1 cut(s) 1111
CfoI GCGC 1 cut(s) 275
Cfr13I GGNCC 2 cut(s) 1037, 1486
Cfr9I CCCGGG 1 cut(s) 1335
Csp6I GTAC 1 cut(s) 160
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 8 cut(s) 54, 295, 363, 974, 1058, 1106, 1155, 1633
DpnI GATC 7 cut(s) 251, 845, 924, 936, 1174, 1368, 1607
DpnII GATC 7 cut(s) 249, 843, 922, 934, 1172, 1366, 1605
DraI TTTAAA 1 cut(s) 240
EaeI YGGCCR 2 cut(s) 3, 1519
Eam1104I CTCTTC 6 cut(s) 90, 200, 206, 1306, 1582, 1663
EarI CTCTTC 6 cut(s) 90, 200, 206, 1306, 1582, 1663
EciI GGCGGA 1 cut(s) 1323
Ecl136II GAGCTC 2 cut(s) 1159, 1262
Eco24I GRGCYC 4 cut(s) 170, 426, 1161, 1264
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 1037
Eco53kI GAGCTC 2 cut(s) 1159, 1262
Eco57I CTGAAG 2 cut(s) 923, 1608
Eco81I CCTNAGG 1 cut(s) 1633
Eco88I CYCGRG 1 cut(s) 1335
EcoICRI GAGCTC 2 cut(s) 1159, 1262
EcoO109I RGGNCCY 1 cut(s) 1037
EcoRI GAATTC 1 cut(s) 207
EcoRII CCWGG 3 cut(s) 1099, 1378, 1497
EcoT38I GRGCYC 4 cut(s) 170, 426, 1161, 1264
FalI AAGNNNNNCTT 2 cut(s) 1396, 1428
FaqI GGGAC 4 cut(s) 32, 530, 1050, 1515
Fnu4HI GCNGC 3 cut(s) 648, 1422, 1640
FokI GGATG 4 cut(s) 1036, 1085, 1676, 1710
FriOI GRGCYC 4 cut(s) 170, 426, 1161, 1264
Fsp4HI GCNGC 3 cut(s) 648, 1422, 1640
FspBI CTAG 5 cut(s) 633, 837, 1239, 1256, 1388
GlaI GCGC 1 cut(s) 274
GluI GCNGC 3 cut(s) 648, 1422, 1640
GsuI CTGGAG 3 cut(s) 603, 1311, 1481
HaeIII GGCC 6 cut(s) 5, 496, 799, 1153, 1488, 1521
HapII CCGG 4 cut(s) 514, 847, 1336, 1694
HhaI GCGC 1 cut(s) 275
Hin6I GCGC 1 cut(s) 273
HinP1I GCGC 1 cut(s) 273
HindIII AAGCTT 2 cut(s) 944, 1010
HinfI GANTC 7 cut(s) 400, 637, 817, 1108, 1574, 1628, 1712
HpaII CCGG 4 cut(s) 514, 847, 1336, 1694
HphI GGTGA 3 cut(s) 96, 1093, 1394
Hpy166II GTNNAC 1 cut(s) 1132
Hpy188III TCNNGA 4 cut(s) 466, 733, 1112, 1571
Hpy8I GTNNAC 1 cut(s) 1132
HpyAV CCTTC 9 cut(s) 60, 406, 520, 541, 872, 939, 1061, 1063, 1106
HpyCH4III ACNGT 5 cut(s) 377, 544, 1136, 1451, 1625
HpyCH4IV ACGT 1 cut(s) 1507
HpyF10VI GCNNNNNNNGC 5 cut(s) 279, 968, 1259, 1384, 1406
HpyF3I CTNAG 8 cut(s) 54, 295, 363, 974, 1058, 1106, 1155, 1633
HpySE526I ACGT 1 cut(s) 1507
HspAI GCGC 1 cut(s) 273
KflI GGGWCCC 1 cut(s) 1037
Kzo9I GATC 7 cut(s) 249, 843, 922, 934, 1172, 1366, 1605
LmnI GCTCC 9 cut(s) 126, 165, 421, 724, 1164, 1259, 1267, 1297, 1587
Lsp1109I GCAGC 3 cut(s) 659, 1408, 1651
LweI GCATC 5 cut(s) 86, 347, 607, 1014, 1261
MaeI CTAG 5 cut(s) 633, 837, 1239, 1256, 1388
MaeII ACGT 1 cut(s) 1507
MaeIII GTNAC 7 cut(s) 458, 538, 849, 1004, 1208, 1234, 1675
MalI GATC 7 cut(s) 251, 845, 924, 936, 1174, 1368, 1607
MboI GATC 7 cut(s) 249, 843, 922, 934, 1172, 1366, 1605
MflI RGATCY 3 cut(s) 843, 922, 1366
MhlI GDGCHC 5 cut(s) 170, 426, 729, 1161, 1264
MlsI TGGCCA 2 cut(s) 5, 1521
MluCI AATT 8 cut(s) 64, 171, 207, 235, 701, 904, 990, 999
MluNI TGGCCA 2 cut(s) 5, 1521
MlyI GAGTC 3 cut(s) 811, 1117, 1568
MmeI TCCRAC 2 cut(s) 821, 1645
Mox20I TGGCCA 2 cut(s) 5, 1521
MscI TGGCCA 2 cut(s) 5, 1521
MseI TTAA 4 cut(s) 239, 870, 1331, 1599
MslI CAYNNNNRTG 1 cut(s) 572
Msp20I TGGCCA 2 cut(s) 5, 1521
MspI CCGG 4 cut(s) 514, 847, 1336, 1694
MspR9I CCNGG 7 cut(s) 515, 1101, 1336, 1337, 1380, 1499, 1694
MvaI CCWGG 3 cut(s) 1101, 1380, 1499
MvnI CGCG 1 cut(s) 273
MwoI GCNNNNNNNGC 5 cut(s) 279, 968, 1259, 1384, 1406
NciI CCSGG 4 cut(s) 515, 1336, 1337, 1694
NdeII GATC 7 cut(s) 249, 843, 922, 934, 1172, 1366, 1605
NlaIV GGNNCC 7 cut(s) 167, 423, 605, 845, 1038, 1039, 1299
NmuCI GTSAC 5 cut(s) 458, 538, 849, 1208, 1234
PagI TCATGA 1 cut(s) 1111
PfeI GAWTC 4 cut(s) 400, 637, 1628, 1712
PflMI CCANNNNNTGG 1 cut(s) 1472
PfoI TCCNGGA 1 cut(s) 1692
PkrI GCNGC 3 cut(s) 649, 1423, 1641
PleI GAGTC 3 cut(s) 811, 1116, 1568
PpsI GAGTC 3 cut(s) 811, 1116, 1568
PpuMI RGGWCCY 1 cut(s) 1037
PsiI TTATAA 1 cut(s) 918
Psp124BI GAGCTC 2 cut(s) 1161, 1264
Psp5II RGGWCCY 1 cut(s) 1037
Psp6I CCWGG 3 cut(s) 1099, 1378, 1497
PspGI CCWGG 3 cut(s) 1099, 1378, 1497
PspN4I GGNNCC 7 cut(s) 167, 423, 605, 845, 1038, 1039, 1299
PspPI GGNCC 2 cut(s) 1037, 1486
PspPPI RGGWCCY 1 cut(s) 1037
PsuI RGATCY 3 cut(s) 843, 922, 1366
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
RseI CAYNNNNRTG 1 cut(s) 572
SacI GAGCTC 2 cut(s) 1161, 1264
SaqAI TTAA 4 cut(s) 239, 870, 1331, 1599
SatI GCNGC 3 cut(s) 648, 1422, 1640
Sau3AI GATC 7 cut(s) 249, 843, 922, 934, 1172, 1366, 1605
Sau96I GGNCC 2 cut(s) 1037, 1486
SchI GAGTC 3 cut(s) 811, 1117, 1568
ScrFI CCNGG 7 cut(s) 515, 1101, 1336, 1337, 1380, 1499, 1694
SduI GDGCHC 5 cut(s) 170, 426, 729, 1161, 1264
SfaNI GCATC 5 cut(s) 86, 347, 607, 1014, 1261
SinI GGWCC 1 cut(s) 1037
SmaI CCCGGG 1 cut(s) 1337
SmiMI CAYNNNNRTG 1 cut(s) 572
SmlI CTYRAG 3 cut(s) 142, 221, 860
SmoI CTYRAG 3 cut(s) 142, 221, 860
Sse9I AATT 8 cut(s) 64, 171, 207, 235, 701, 904, 990, 999
SsiI CCGC 3 cut(s) 865, 1016, 1308
SspMI CTAG 5 cut(s) 633, 837, 1239, 1256, 1388
SstI GAGCTC 2 cut(s) 1161, 1264
StyD4I CCNGG 7 cut(s) 513, 1099, 1334, 1335, 1378, 1497, 1692
TaaI ACNGT 5 cut(s) 377, 544, 1136, 1451, 1625
TaiI ACGT 1 cut(s) 1510
TaqI TCGA 1 cut(s) 615
TasI AATT 8 cut(s) 64, 171, 207, 235, 701, 904, 990, 999
TatI WGTACW 1 cut(s) 159
TfiI GAWTC 4 cut(s) 400, 637, 1628, 1712
Tru1I TTAA 4 cut(s) 239, 870, 1331, 1599
Tru9I TTAA 4 cut(s) 239, 870, 1331, 1599
TscAI CASTG 7 cut(s) 549, 978, 1141, 1217, 1456, 1630, 1714
TseFI GTSAC 5 cut(s) 458, 538, 849, 1208, 1234
TseI GCWGC 3 cut(s) 647, 1421, 1639
Tsp45I GTSAC 5 cut(s) 458, 538, 849, 1208, 1234
TspDTI ATGAA 8 cut(s) 708, 815, 956, 1043, 1128, 1206, 1405, 1450
TspGWI ACGGA 1 cut(s) 746
TspMI CCCGGG 1 cut(s) 1335
TspRI CASTG 7 cut(s) 549, 978, 1141, 1217, 1456, 1630, 1714
Van91I CCANNNNNTGG 1 cut(s) 1472
VpaK11BI GGWCC 1 cut(s) 1037
XapI RAATTY 2 cut(s) 207, 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 177
XmaI CCCGGG 1 cut(s) 1335
XspI CTAG 5 cut(s) 633, 837, 1239, 1256, 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.