Rh1BG013000

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0005122
Physical Location & Seq
Reverse (-)
1604829 .. 1608478
3650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG013000.1

Sequence Viewer

Length: 1731 bp
ATGGCCAAGTCAAAAAGCAACAAGAACAAGGTCTCTTACATTTCAGTCCCTTCTCAGATAATCAGTTCTTTGTCTGCATCTTCTCTTCAATCCCTCCTTACCTCACCCAAGAAATCATGTTCAAGGAGCAAAGCAAGGTTCTTGAGCTTGCTGACCAAGTACAGGAGCCCAAGTTTCTGGGTTTTCTTCCTCTTCCTCTTCCTCTTTGGAATTCTTGGTATGCTCAAGTTGAGATTCAATTTAAACACTTTGATCCCTTATTCTCCTTACCCACGCGCCACAAGCCAGTTCCACGCCTTAGTCTCCTCAAATGGGTATTTGAAATCACACACGAGTATTGCATCAAACGAAAATGGTCAGAAAGCTGAGGTCTTTGACAGTTTCAGCCATTCGCTTCCCTTGATTCTAAAGGAGGAGGAGGAGGAGCCCAAATCAGAACCAAACAGTGACAAGGCAATAAGTGACTTCTGGAAGCAACCAGATGGTTTGGGGTATAGGCCATGCTTGGATTTCAGCCGGGACTATCAGAAGGGAAGTGTAGTGACAGTGAAGGACAGGACTAAATACCTCATGGTTGTGGTTGCTGGAGGGCTGAACCAGCAAAGGAACCAGATTGTCGATGCTGTGGTTATTGCTAGGATTCTTGGGGCAGCTTTGGTCGTTCCCATTTTGCAAGTCAATGTCATCTGGAGAGATGAAAGTCAATTTTCTGATATATTTGATATGGAGCACTTCAAGAAAGTTCTTGCTAATGATGTCCGTATAGTTTCTTCACTGCCATCAACACATACAATGACAAGGCCAGTGGAGAAGAAAGGGACTCCTTTTCATGTTTCTCCTAGTTGGATCCGGTCACGCTATCTCAAGCGGAGAAGGAGTTCTGCTTCTTGGAGGCTTCAATTCAAGGCTTCTATGGATCTTCCTTCTGATCTTCAGAAGCTTCGTTGCAAGGTTGCTTTTCATGCATTGAGGTTTGCTCCTCCAATTTTGGGAATTGGTAACAAGCTTGCGGAGAGGATGCGTAGCAAGGGACCCTACCTTGCCCTTCATCTAAGAATGGAGAAGGATGTATGGGTGAGGACTGGTTGCCTTCCTGGTCTGAGTCATGAATACGATGAGATAGTAAACAGTGAAAGGATACAACGGCCTGAGCTCCTAACTGCAAGATCAAACATGACTTACCATGAAAGAAAACTTGCTGGTCACTGCCCCTTGAACGCCGTGGAAGTGACTAGGTTGCTAAAAGCTCTAGGAGCTCCCAAAGATGCAAGAATATATTGGGCTGGAGGGGAGCCATTGGGTGGAAGAGAAGCCTTGCTACCATTAACGCGGGAGTTTTCCCATTTTTACAATAAGGAAGATCTTGCTTTGCCTGGTGAGCTAGAACCTTTTGCAAAAAGAGCTTCTTTCATGGCTGCCATTGATTACATAGTTTCCGAGAACAGTGATGTCTTCATGCCATCTCATGGAGGAAATATGGGCCATGCACTCCAGGGACAACGTGCCTATGCTGGCCACAAGAAGAATATAACACCAGACAAAAGGCATATGCTTCCTTATTTTATGGACTCTTCGCTCCCTGATGTAGAGTTTAATAGGATCATAAAGGAGTTGCACAGTGATTCCTTAGGGCAGCCAGACATTCGGATTAGTAAAGTTGGAAGAGATGTTACAAAGTATCCCATCCCGGAGTGTATGTGCAGTGATTCTCATGCTCATCCCCACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

576

Amino Acids

65.1

Weight (kDa)

9.59

Isoelectric Point (pI)

47.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 189 - 503 4e-81 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 1301, 1466
AccII CGCG 2 cut(s) 276, 1330
AciI CCGC 3 cut(s) 868, 1010, 1330
AclWI GGATC 5 cut(s) 247, 841, 854, 924, 1607
AcoI YGGCCR 2 cut(s) 3, 1513
AcsI RAATTY 1 cut(s) 210
AcuI CTGAAG 1 cut(s) 917
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 5 cut(s) 162, 312, 989, 1301, 1466
AgsI TTSAA 8 cut(s) 89, 123, 238, 322, 736, 899, 904, 1216
AjnI CCWGG 3 cut(s) 1093, 1372, 1491
AloI GAACNNNNNNTCC 2 cut(s) 862, 894
Alw21I GWGCWC 3 cut(s) 732, 1155, 1258
Alw26I GTCTC 2 cut(s) 37, 307
AlwI GGATC 5 cut(s) 247, 841, 854, 924, 1607
AoxI GGCC 6 cut(s) 3, 497, 800, 1145, 1480, 1513
ApeKI GCWGC 3 cut(s) 650, 1415, 1633
ApoI RAATTY 1 cut(s) 210
Asp700I GAANNNNTTC 1 cut(s) 877
AspLEI GCGC 1 cut(s) 278
AspS9I GGNCC 2 cut(s) 1031, 1480
AsuC2I CCSGG 2 cut(s) 518, 1688
AsuHPI GGTGA 3 cut(s) 96, 1087, 1388
AvaII GGWCC 1 cut(s) 1031
AxyI CCTNAGG 1 cut(s) 1627
BalI TGGCCA 2 cut(s) 5, 1515
BamHI GGATCC 1 cut(s) 846
BanII GRGCYC 4 cut(s) 170, 429, 1155, 1258
BarI GAAGNNNNNNTAC 6 cut(s) 754, 786, 1302, 1334, 1654, 1686
BauI CACGAG 1 cut(s) 331
BbsI GAAGAC 1 cut(s) 1444
Bbv12I GWGCWC 3 cut(s) 732, 1155, 1258
BbvCI CCTCAGC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 662, 1402, 1645
BccI CCATC 4 cut(s) 476, 787, 1468, 1691
BceAI ACGGC 2 cut(s) 1160, 1205
BciT130I CCWGG 3 cut(s) 1095, 1374, 1493
BciVI GTATCC 2 cut(s) 1131, 1689
BcnI CCSGG 2 cut(s) 518, 1688
BcoDI GTCTC 2 cut(s) 37, 307
BfaI CTAG 5 cut(s) 636, 840, 1233, 1250, 1382
BfuI GTATCC 2 cut(s) 1131, 1689
BglII AGATCT 1 cut(s) 1360
BisI GCNGC 3 cut(s) 651, 1416, 1634
BlsI GCNGC 3 cut(s) 652, 1417, 1635
Bme1390I CCNGG 5 cut(s) 518, 1095, 1374, 1493, 1688
Bme18I GGWCC 1 cut(s) 1031
BmgT120I GGNCC 2 cut(s) 1031, 1480
BmiI GGNNCC 7 cut(s) 167, 426, 608, 848, 1032, 1033, 1293
BmrFI CCNGG 5 cut(s) 518, 1095, 1374, 1493, 1688
BmsI GCATC 5 cut(s) 86, 350, 610, 1008, 1255
BpiI GAAGAC 1 cut(s) 1444
BplI GAGNNNNNCTC 2 cut(s) 961, 993
BpmI CTGGAG 4 cut(s) 606, 709, 1305, 1475
Bpu10I CCTNAGC 2 cut(s) 366, 1149
BpuEI CTTGAG 3 cut(s) 163, 209, 848
BpuMI CCSGG 2 cut(s) 518, 1688
BsaI GGTCTC 1 cut(s) 37
BsaJI CCNNGG 2 cut(s) 1221, 1492
BsaWI WCCGGW 1 cut(s) 849
BsaXI ACNNNNNCTCC 6 cut(s) 157, 187, 862, 892, 1682, 1712
Bsc4I CCNNNNNNNGG 5 cut(s) 162, 312, 989, 1301, 1466
Bse1I ACTGG 3 cut(s) 286, 803, 1087
Bse21I CCTNAGG 1 cut(s) 1627
BseBI CCWGG 3 cut(s) 1095, 1374, 1493
BseDI CCNNGG 2 cut(s) 1221, 1492
BseGI GGATG 4 cut(s) 1023, 1072, 1683, 1717
BseLI CCNNNNNNNGG 5 cut(s) 162, 312, 989, 1301, 1466
BseMII CTCAG 4 cut(s) 68, 357, 1091, 1140
BseNI ACTGG 3 cut(s) 286, 803, 1087
BseRI GAGGAG 6 cut(s) 295, 428, 431, 434, 437, 969
BseXI GCAGC 3 cut(s) 662, 1402, 1645
BsgI GTGCAG 1 cut(s) 1720
Bsh1236I CGCG 2 cut(s) 276, 1330
BshFI GGCC 6 cut(s) 5, 499, 802, 1147, 1482, 1515
BsiHKAI GWGCWC 3 cut(s) 732, 1155, 1258
BsiSI CCGG 3 cut(s) 517, 850, 1688
BslFI GGGAC 5 cut(s) 32, 533, 832, 1044, 1509
BslI CCNNNNNNNGG 5 cut(s) 162, 312, 989, 1301, 1466
BsmAI GTCTC 2 cut(s) 37, 307
BsmFI GGGAC 5 cut(s) 32, 533, 832, 1044, 1509
BsnI GGCC 6 cut(s) 5, 499, 802, 1147, 1482, 1515
Bso31I GGTCTC 1 cut(s) 37
Bsp1286I GDGCHC 5 cut(s) 170, 429, 732, 1155, 1258
Bsp143I GATC 7 cut(s) 252, 846, 916, 928, 1166, 1360, 1599
BspACI CCGC 3 cut(s) 868, 1010, 1330
BspANI GGCC 6 cut(s) 5, 499, 802, 1147, 1482, 1515
BspCNI CTCAG 4 cut(s) 67, 358, 1092, 1141
BspFNI CGCG 2 cut(s) 276, 1330
BspHI TCATGA 1 cut(s) 1105
BspLI GGNNCC 7 cut(s) 167, 426, 608, 848, 1032, 1033, 1293
BspPI GGATC 5 cut(s) 247, 841, 854, 924, 1607
BspTNI GGTCTC 1 cut(s) 37
BsrI ACTGG 3 cut(s) 286, 803, 1087
BssECI CCNNGG 2 cut(s) 1221, 1492
BssMI GATC 7 cut(s) 252, 846, 916, 928, 1166, 1360, 1599
BssSI CACGAG 1 cut(s) 331
Bst2BI CACGAG 1 cut(s) 331
Bst2UI CCWGG 3 cut(s) 1095, 1374, 1493
Bst4CI ACNGT 6 cut(s) 380, 446, 547, 1130, 1445, 1619
Bst6I CTCTTC 6 cut(s) 90, 197, 203, 1300, 1576, 1657
BstC8I GCNNGC 3 cut(s) 149, 1008, 1513
BstDEI CTNAG 7 cut(s) 54, 298, 366, 1052, 1100, 1149, 1627
BstDSI CCRYGG 1 cut(s) 1221
BstF5I GGATG 4 cut(s) 1023, 1072, 1683, 1717
BstFNI CGCG 2 cut(s) 276, 1330
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 7 cut(s) 255, 849, 919, 931, 1169, 1363, 1602
BstMAI GTCTC 2 cut(s) 37, 307
BstMBI GATC 7 cut(s) 252, 846, 916, 928, 1166, 1360, 1599
BstMWI GCNNNNNNNGC 6 cut(s) 282, 598, 962, 1253, 1378, 1400
BstNI CCWGG 3 cut(s) 1095, 1374, 1493
BstSCI CCNGG 5 cut(s) 516, 1093, 1372, 1491, 1686
BstUI CGCG 2 cut(s) 276, 1330
BstV1I GCAGC 3 cut(s) 662, 1402, 1645
BstV2I GAAGAC 1 cut(s) 1444
BstX2I RGATCY 3 cut(s) 846, 916, 1360
BstXI CCANNNNNNTGG 1 cut(s) 177
BstYI RGATCY 3 cut(s) 846, 916, 1360
Bsu36I CCTNAGG 1 cut(s) 1627
BsuI GTATCC 2 cut(s) 1131, 1689
BsuRI GGCC 6 cut(s) 5, 499, 802, 1147, 1482, 1515
BtgI CCRYGG 1 cut(s) 1221
BtsCI GGATG 4 cut(s) 1023, 1072, 1683, 1717
BtsI GCAGTG 3 cut(s) 773, 1204, 1708
BtsIMutI CAGTG 9 cut(s) 451, 552, 773, 810, 1135, 1204, 1450, 1624, 1708
Cac8I GCNNGC 3 cut(s) 149, 1008, 1513
CciI TCATGA 1 cut(s) 1105
CfoI GCGC 1 cut(s) 278
Cfr13I GGNCC 2 cut(s) 1031, 1480
Csp6I GTAC 1 cut(s) 160
CspCI CAANNNNNGTGG 2 cut(s) 786, 821
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 7 cut(s) 54, 298, 366, 1052, 1100, 1149, 1627
DpnI GATC 7 cut(s) 254, 848, 918, 930, 1168, 1362, 1601
DpnII GATC 7 cut(s) 252, 846, 916, 928, 1166, 1360, 1599
DraI TTTAAA 1 cut(s) 243
EaeI YGGCCR 2 cut(s) 3, 1513
Eam1104I CTCTTC 6 cut(s) 90, 197, 203, 1300, 1576, 1657
EarI CTCTTC 6 cut(s) 90, 197, 203, 1300, 1576, 1657
Ecl136II GAGCTC 2 cut(s) 1153, 1256
Eco24I GRGCYC 4 cut(s) 170, 429, 1155, 1258
Eco31I GGTCTC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 1031
Eco53kI GAGCTC 2 cut(s) 1153, 1256
Eco57I CTGAAG 1 cut(s) 917
Eco81I CCTNAGG 1 cut(s) 1627
EcoICRI GAGCTC 2 cut(s) 1153, 1256
EcoO109I RGGNCCY 1 cut(s) 1031
EcoRI GAATTC 1 cut(s) 210
EcoRII CCWGG 3 cut(s) 1093, 1372, 1491
EcoT22I ATGCAT 1 cut(s) 967
EcoT38I GRGCYC 4 cut(s) 170, 429, 1155, 1258
FalI AAGNNNNNCTT 2 cut(s) 1390, 1422
FaqI GGGAC 5 cut(s) 32, 533, 832, 1044, 1509
FauI CCCGC 1 cut(s) 1323
FauNDI CATATG 1 cut(s) 1548
Fnu4HI GCNGC 3 cut(s) 651, 1416, 1634
FokI GGATG 4 cut(s) 1030, 1079, 1670, 1704
FriOI GRGCYC 4 cut(s) 170, 429, 1155, 1258
Fsp4HI GCNGC 3 cut(s) 651, 1416, 1634
FspBI CTAG 5 cut(s) 636, 840, 1233, 1250, 1382
GlaI GCGC 1 cut(s) 277
GluI GCNGC 3 cut(s) 651, 1416, 1634
GsuI CTGGAG 4 cut(s) 606, 709, 1305, 1475
HaeIII GGCC 6 cut(s) 5, 499, 802, 1147, 1482, 1515
HapII CCGG 3 cut(s) 517, 850, 1688
HhaI GCGC 1 cut(s) 278
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HindIII AAGCTT 2 cut(s) 938, 1004
HinfI GANTC 8 cut(s) 234, 403, 640, 820, 1102, 1568, 1622, 1706
HpaII CCGG 3 cut(s) 517, 850, 1688
HphI GGTGA 3 cut(s) 96, 1087, 1388
Hpy166II GTNNAC 1 cut(s) 1126
Hpy188III TCNNGA 5 cut(s) 142, 469, 688, 736, 1106
Hpy8I GTNNAC 1 cut(s) 1126
HpyAV CCTTC 8 cut(s) 60, 523, 544, 867, 933, 1055, 1057, 1100
HpyCH4III ACNGT 6 cut(s) 380, 446, 547, 1130, 1445, 1619
HpyCH4IV ACGT 1 cut(s) 1501
HpyF10VI GCNNNNNNNGC 6 cut(s) 282, 598, 962, 1253, 1378, 1400
HpyF3I CTNAG 7 cut(s) 54, 298, 366, 1052, 1100, 1149, 1627
HpySE526I ACGT 1 cut(s) 1501
HspAI GCGC 1 cut(s) 276
KflI GGGWCCC 1 cut(s) 1031
Kzo9I GATC 7 cut(s) 252, 846, 916, 928, 1166, 1360, 1599
Lsp1109I GCAGC 3 cut(s) 662, 1402, 1645
LweI GCATC 5 cut(s) 86, 350, 610, 1008, 1255
MaeI CTAG 5 cut(s) 636, 840, 1233, 1250, 1382
MaeII ACGT 1 cut(s) 1501
MaeIII GTNAC 8 cut(s) 446, 461, 541, 852, 998, 1202, 1228, 1669
MalI GATC 7 cut(s) 254, 848, 918, 930, 1168, 1362, 1601
MboI GATC 7 cut(s) 252, 846, 916, 928, 1166, 1360, 1599
MflI RGATCY 3 cut(s) 846, 916, 1360
MhlI GDGCHC 5 cut(s) 170, 429, 732, 1155, 1258
MlsI TGGCCA 2 cut(s) 5, 1515
MluCI AATT 6 cut(s) 210, 238, 704, 899, 984, 993
MluNI TGGCCA 2 cut(s) 5, 1515
MlyI GAGTC 3 cut(s) 814, 1111, 1562
MmeI TCCRAC 2 cut(s) 824, 1639
Mox20I TGGCCA 2 cut(s) 5, 1515
Mph1103I ATGCAT 1 cut(s) 967
MroXI GAANNNNTTC 1 cut(s) 877
MscI TGGCCA 2 cut(s) 5, 1515
MseI TTAA 3 cut(s) 242, 1325, 1593
MslI CAYNNNNRTG 1 cut(s) 575
Msp20I TGGCCA 2 cut(s) 5, 1515
MspI CCGG 3 cut(s) 517, 850, 1688
MspR9I CCNGG 5 cut(s) 518, 1095, 1374, 1493, 1688
MvaI CCWGG 3 cut(s) 1095, 1374, 1493
MvnI CGCG 2 cut(s) 276, 1330
MwoI GCNNNNNNNGC 6 cut(s) 282, 598, 962, 1253, 1378, 1400
NciI CCSGG 2 cut(s) 518, 1688
NdeI CATATG 1 cut(s) 1548
NdeII GATC 7 cut(s) 252, 846, 916, 928, 1166, 1360, 1599
NlaIV GGNNCC 7 cut(s) 167, 426, 608, 848, 1032, 1033, 1293
NmuCI GTSAC 6 cut(s) 446, 461, 541, 852, 1202, 1228
NsiI ATGCAT 1 cut(s) 967
PagI TCATGA 1 cut(s) 1105
PdmI GAANNNNTTC 1 cut(s) 877
PfeI GAWTC 5 cut(s) 234, 403, 640, 1622, 1706
PflMI CCANNNNNTGG 2 cut(s) 1301, 1466
PfoI TCCNGGA 1 cut(s) 1686
PkrI GCNGC 3 cut(s) 652, 1417, 1635
PleI GAGTC 3 cut(s) 814, 1110, 1562
PpsI GAGTC 3 cut(s) 814, 1110, 1562
PpuMI RGGWCCY 1 cut(s) 1031
Psp124BI GAGCTC 2 cut(s) 1155, 1258
Psp5II RGGWCCY 1 cut(s) 1031
Psp6I CCWGG 3 cut(s) 1093, 1372, 1491
PspGI CCWGG 3 cut(s) 1093, 1372, 1491
PspN4I GGNNCC 7 cut(s) 167, 426, 608, 848, 1032, 1033, 1293
PspPI GGNCC 2 cut(s) 1031, 1480
PspPPI RGGWCCY 1 cut(s) 1031
PsuI RGATCY 3 cut(s) 846, 916, 1360
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
RseI CAYNNNNRTG 1 cut(s) 575
SacI GAGCTC 2 cut(s) 1155, 1258
SaqAI TTAA 3 cut(s) 242, 1325, 1593
SatI GCNGC 3 cut(s) 651, 1416, 1634
Sau3AI GATC 7 cut(s) 252, 846, 916, 928, 1166, 1360, 1599
Sau96I GGNCC 2 cut(s) 1031, 1480
SchI GAGTC 3 cut(s) 814, 1111, 1562
ScrFI CCNGG 5 cut(s) 518, 1095, 1374, 1493, 1688
SduI GDGCHC 5 cut(s) 170, 429, 732, 1155, 1258
SfaNI GCATC 5 cut(s) 86, 350, 610, 1008, 1255
SinI GGWCC 1 cut(s) 1031
SmiMI CAYNNNNRTG 1 cut(s) 575
SmlI CTYRAG 3 cut(s) 142, 224, 863
SmoI CTYRAG 3 cut(s) 142, 224, 863
Sse9I AATT 6 cut(s) 210, 238, 704, 899, 984, 993
SsiI CCGC 3 cut(s) 868, 1010, 1330
SspMI CTAG 5 cut(s) 636, 840, 1233, 1250, 1382
SstI GAGCTC 2 cut(s) 1155, 1258
StyD4I CCNGG 5 cut(s) 516, 1093, 1372, 1491, 1686
TaaI ACNGT 6 cut(s) 380, 446, 547, 1130, 1445, 1619
TaiI ACGT 1 cut(s) 1504
TaqI TCGA 1 cut(s) 618
TasI AATT 6 cut(s) 210, 238, 704, 899, 984, 993
TatI WGTACW 1 cut(s) 159
TfiI GAWTC 5 cut(s) 234, 403, 640, 1622, 1706
Tru1I TTAA 3 cut(s) 242, 1325, 1593
Tru9I TTAA 3 cut(s) 242, 1325, 1593
TscAI CASTG 9 cut(s) 451, 552, 780, 810, 1135, 1211, 1450, 1624, 1708
TseFI GTSAC 6 cut(s) 446, 461, 541, 852, 1202, 1228
TseI GCWGC 3 cut(s) 650, 1415, 1633
Tsp45I GTSAC 6 cut(s) 446, 461, 541, 852, 1202, 1228
TspDTI ATGAA 8 cut(s) 711, 818, 950, 1037, 1122, 1200, 1399, 1444
TspGWI ACGGA 1 cut(s) 749
TspRI CASTG 9 cut(s) 451, 552, 780, 810, 1135, 1211, 1450, 1624, 1708
Van91I CCANNNNNTGG 2 cut(s) 1301, 1466
VpaK11BI GGWCC 1 cut(s) 1031
XapI RAATTY 1 cut(s) 210
XmnI GAANNNNTTC 1 cut(s) 877
XspI CTAG 5 cut(s) 636, 840, 1233, 1250, 1382
Zsp2I ATGCAT 1 cut(s) 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.