AT3G07900

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005122
Physical Location & Seq
Forward (+)
2520701 .. 2523115
2415 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G07900.1

Sequence Viewer

Length: 1740 bp
ATGGGAACATGGAAGAACAAAAACAGTAATAAGAAGAACAGAGTTAGCTACATTTCAGTCCCAGCTCAGATCATAAACTCAGTTTCATCATCATCTTTACACAAATTTCTCGATAATAAATCTTCAAAGAAGAACACAAGCAAGTTCTTCTTCAATCTCAGAAACCCAAAGCTCTGGGCTTTCTCTCTCTTCTTACTTTCTATTTTGGGGATATCATTAAGACTCGGTCTTTGTCTTTCTCATTTCGGTTCTGGCGATCACGAAAGTCAACTTCAGAGTTCAGATTCTAATGGCTCGCCCAAATCCCATATGGGTTTTGCGTATATCCGATCAAGCACTACTCAAGTTGAGATTTCAAACGCAAAGGATCGATCTTTAGATATGGGAGTGGAGAAAAATGAAACCTTTGGTGGTCACCAATCACATTTGATCATCCCTAATGGAAATGGACATGATGATAAGAATTATGACTTCTGGAAACAGCCTGATGGATTAGGTTACAAGCCTTGCTTAGATTTCAGCATTGAATACAGAAGAGAGAGTAAGAAGATTCTTGTAGAGAGGAGAAAGTATCTGATGGTGGTTGTCTCTGGTGGGTTGAATCAGCAGAAGATTCAGATTGTTGATGCAGTTGTGATTGCGAGGATTCTAGGTGCTGTTCTTGTTGTCCCAATATTGCAAATCAATCTCATTTGGGGTGACGAGAGCGAGTTTTCAGATATATTTGATTTAGAGCAATTCAAGAGTGTTTTAGCAAATGATGTGAAGATTGTTTCGTTGCTTCCCGCAAGTAAAGTAATGACTAGACCATCGGAAGATGGTAGTATGCCCTTTAATGCCTCCCCTCAATGGATCCGTTCGCACTATCCAAAGCGTTTCAATAGGGAAGGTGTTCTGCTTTTAAGGAGATTGGATTCAAGATTGTCTAAAGACTTACCCTCTGATCTCCAGAAGCTCCGTTGTAAGGTAGCATTTGAAGCTCTAAAGTTTTCGCCACGAGTTATGGAAATGGGGACGAAGCTTGCAGAGAGGATGAGGAGCAAAGGGCCTTACATTGCGTTGCATCTTCGGATGGAGAAAGATGTGTGGGTGAGAACAGGCTGCCTTTCTGGTTTGAGCTCAAAGTATGATGAGATTGTGAACATTGAAAGGATTAAGCGGCCTGAGCTCTTAACAGCCAAATCTAGTATGACATCTAACGAGAGAAAACTCGCGGGTCTCTGTCCGTTAAATGCCAAGGAAGTAACAAGGCTGCTTAGAGCTCTTGGAGCACCAAGAGATGCGAGGATCTATTGGGCGGGAGGGGAACCGCTCGGTGGAAAGGAAGCTTTGAAACCGTTAACAAGTGAATTCCCACATCTCTACAACAAGTACGATATTGCGTTGCCTCTTGAACTCAAACCTTTCGCAAAACGAGCTTCGATAATGGCAGCAATCGACTATATAGTGTGTAAGGAGAGTGATGTGTTTATGGCATCTCATGGAGGGAACATGGGCCACGCAATTCAGGGGCACAGGGCTTATGAAGGACACAAGAAGATTATAACGCCAAACAAAAGACATATGCTACCATACTTTGTGAACTCATCATTGACTGAAACAGAGTTTGAGAAGATGATAAAGAAATTACACAGACAATCTCTAGGACAGCCAGAACTAAGGATTAGCAAAGCTGGAAGAGATGTTACAAAGTACCCTGTTCCTGAGTGTATGTGCAATCAATCCAACACCACTATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

579

Amino Acids

65.36

Weight (kDa)

9.65

Isoelectric Point (pI)

50.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 191 - 508 2.8e-86 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1544
AccBSI CCGCTC 1 cut(s) 1312
AccII CGCG 1 cut(s) 1214
AciI CCGC 5 cut(s) 786, 1159, 1214, 1298, 1310
AclWI GGATC 4 cut(s) 375, 847, 860, 1295
AcsI RAATTY 2 cut(s) 104, 1349
AcuI CTGAAG 1 cut(s) 257
AfaI GTAC 2 cut(s) 1373, 1694
AfiI CCNNNNNNNGG 3 cut(s) 849, 964, 1316
Alw21I GWGCWC 4 cut(s) 1121, 1170, 1264, 1273
Alw26I GTCTC 2 cut(s) 592, 1223
AlwI GGATC 4 cut(s) 375, 847, 860, 1295
AoxI GGCC 3 cut(s) 1046, 1160, 1495
ApeKI GCWGC 3 cut(s) 1101, 1252, 1430
ApoI RAATTY 2 cut(s) 104, 1349
ArsI GACNNNNNNTTYG 2 cut(s) 213, 245
Asp700I GAANNNNTTC 1 cut(s) 891
AspS9I GGNCC 2 cut(s) 1046, 1495
AsuHPI GGTGA 3 cut(s) 407, 710, 1102
BaeGI GKGCMC 1 cut(s) 1515
BamHI GGATCC 1 cut(s) 852
BanII GRGCYC 3 cut(s) 1121, 1170, 1264
BarI GAAGNNNNNNTAC 2 cut(s) 1669, 1701
BauI CACGAG 1 cut(s) 996
Bbv12I GWGCWC 4 cut(s) 1121, 1170, 1264, 1273
BbvI GCAGC 3 cut(s) 1088, 1239, 1442
BccI CCATC 5 cut(s) 482, 571, 812, 817, 1066
BclI TGATCA 1 cut(s) 429
BcoDI GTCTC 2 cut(s) 592, 1223
BfaI CTAG 4 cut(s) 650, 804, 1185, 1643
BisI GCNGC 4 cut(s) 1102, 1160, 1253, 1431
BlsI GCNGC 4 cut(s) 1103, 1161, 1254, 1432
BmgT120I GGNCC 2 cut(s) 1046, 1495
BmiI GGNNCC 2 cut(s) 854, 1308
BmsI GCATC 4 cut(s) 616, 1072, 1270, 1484
BpmI CTGGAG 1 cut(s) 932
Bpu10I CCTNAGC 1 cut(s) 1164
BpuEI CTTGAG 1 cut(s) 327
Bsa29I ATCGAT 1 cut(s) 370
BsaI GGTCTC 1 cut(s) 1223
BsaJI CCNNGG 1 cut(s) 1236
Bsc4I CCNNNNNNNGG 3 cut(s) 849, 964, 1316
Bse3DI GCAATG 1 cut(s) 1053
BseCI ATCGAT 1 cut(s) 370
BseDI CCNNGG 1 cut(s) 1236
BseGI GGATG 3 cut(s) 432, 1038, 1077
BseLI CCNNNNNNNGG 3 cut(s) 849, 964, 1316
BseMI GCAATG 1 cut(s) 1053
BseMII CTCAG 5 cut(s) 80, 93, 172, 1155, 1695
BseRI GAGGAG 2 cut(s) 577, 1051
BseSI GKGCMC 1 cut(s) 1515
BseXI GCAGC 3 cut(s) 1088, 1239, 1442
BseYI CCCAGC 1 cut(s) 61
Bsh1236I CGCG 1 cut(s) 1214
BshFI GGCC 3 cut(s) 1048, 1162, 1497
BshVI ATCGAT 1 cut(s) 370
BsiHKAI GWGCWC 4 cut(s) 1121, 1170, 1264, 1273
BslFI GGGAC 3 cut(s) 44, 653, 1027
BslI CCNNNNNNNGG 3 cut(s) 849, 964, 1316
BsmAI GTCTC 2 cut(s) 592, 1223
BsmFI GGGAC 3 cut(s) 44, 653, 1027
BsnI GGCC 3 cut(s) 1048, 1162, 1497
Bso31I GGTCTC 1 cut(s) 1223
Bsp1286I GDGCHC 5 cut(s) 1121, 1170, 1264, 1273, 1515
Bsp143I GATC 9 cut(s) 69, 256, 329, 367, 371, 429, 852, 943, 1287
BspACI CCGC 5 cut(s) 786, 1159, 1214, 1298, 1310
BspANI GGCC 3 cut(s) 1048, 1162, 1497
BspCNI CTCAG 5 cut(s) 79, 92, 171, 1156, 1696
BspDI ATCGAT 1 cut(s) 370
BspFNI CGCG 1 cut(s) 1214
BspLI GGNNCC 2 cut(s) 854, 1308
BspPI GGATC 4 cut(s) 375, 847, 860, 1295
BspTNI GGTCTC 1 cut(s) 1223
BsrBI CCGCTC 1 cut(s) 1312
BsrDI GCAATG 1 cut(s) 1053
BssECI CCNNGG 1 cut(s) 1236
BssMI GATC 9 cut(s) 69, 256, 329, 367, 371, 429, 852, 943, 1287
BssSI CACGAG 1 cut(s) 996
BssT1I CCWWGG 1 cut(s) 1236
Bst2BI CACGAG 1 cut(s) 996
Bst4CI ACNGT 2 cut(s) 26, 1338
Bst6I CTCTTC 3 cut(s) 194, 529, 1672
BstC8I GCNNGC 2 cut(s) 296, 1023
BstDEI CTNAG 8 cut(s) 66, 79, 158, 511, 1164, 1256, 1658, 1704
BstEII GGTNACC 1 cut(s) 413
BstF5I GGATG 3 cut(s) 432, 1038, 1077
BstFNI CGCG 1 cut(s) 1214
BstKTI GATC 9 cut(s) 72, 259, 332, 370, 374, 432, 855, 946, 1290
BstMAI GTCTC 2 cut(s) 592, 1223
BstMBI GATC 9 cut(s) 69, 256, 329, 367, 371, 429, 852, 943, 1287
BstMWI GCNNNNNNNGC 4 cut(s) 977, 1165, 1268, 1415
BstPI GGTNACC 1 cut(s) 413
BstSLI GKGCMC 1 cut(s) 1515
BstUI CGCG 1 cut(s) 1214
BstV1I GCAGC 3 cut(s) 1088, 1239, 1442
BstX2I RGATCY 2 cut(s) 852, 1287
BstXI CCANNNNNNTGG 1 cut(s) 174
BstYI RGATCY 2 cut(s) 852, 1287
Bsu15I ATCGAT 1 cut(s) 370
BsuRI GGCC 3 cut(s) 1048, 1162, 1497
BsuTUI ATCGAT 1 cut(s) 370
BtsCI GGATG 3 cut(s) 432, 1038, 1077
Cac8I GCNNGC 2 cut(s) 296, 1023
Cfr13I GGNCC 2 cut(s) 1046, 1495
ClaI ATCGAT 1 cut(s) 370
Csp6I GTAC 2 cut(s) 1372, 1693
CviAII CATG 4 cut(s) 9, 452, 1481, 1492
CviQI GTAC 2 cut(s) 1372, 1693
DdeI CTNAG 8 cut(s) 66, 79, 158, 511, 1164, 1256, 1658, 1704
DpnI GATC 9 cut(s) 71, 258, 331, 369, 373, 431, 854, 945, 1289
DpnII GATC 9 cut(s) 69, 256, 329, 367, 371, 429, 852, 943, 1287
Eam1104I CTCTTC 3 cut(s) 194, 529, 1672
EarI CTCTTC 3 cut(s) 194, 529, 1672
Ecl136II GAGCTC 3 cut(s) 1119, 1168, 1262
Eco130I CCWWGG 1 cut(s) 1236
Eco24I GRGCYC 3 cut(s) 1121, 1170, 1264
Eco31I GGTCTC 1 cut(s) 1223
Eco32I GATATC 1 cut(s) 213
Eco53kI GAGCTC 3 cut(s) 1119, 1168, 1262
Eco57I CTGAAG 1 cut(s) 257
Eco91I GGTNACC 1 cut(s) 413
EcoICRI GAGCTC 3 cut(s) 1119, 1168, 1262
EcoO109I RGGNCCY 1 cut(s) 1046
EcoO65I GGTNACC 1 cut(s) 413
EcoRI GAATTC 1 cut(s) 1349
EcoRV GATATC 1 cut(s) 213
EcoT14I CCWWGG 1 cut(s) 1236
EcoT38I GRGCYC 3 cut(s) 1121, 1170, 1264
ErhI CCWWGG 1 cut(s) 1236
FaeI CATG 4 cut(s) 12, 455, 1484, 1495
FalI AAGNNNNNCTT 4 cut(s) 134, 166, 494, 526
FaqI GGGAC 3 cut(s) 44, 653, 1027
FatI CATG 4 cut(s) 8, 451, 1480, 1491
FauI CCCGC 3 cut(s) 793, 1207, 1291
FauNDI CATATG 2 cut(s) 309, 1563
FbaI TGATCA 1 cut(s) 429
Fnu4HI GCNGC 4 cut(s) 1102, 1160, 1253, 1431
FokI GGATG 3 cut(s) 419, 1045, 1084
FriOI GRGCYC 3 cut(s) 1121, 1170, 1264
Fsp4HI GCNGC 4 cut(s) 1102, 1160, 1253, 1431
FspBI CTAG 4 cut(s) 650, 804, 1185, 1643
GluI GCNGC 4 cut(s) 1102, 1160, 1253, 1431
GsaI CCCAGC 1 cut(s) 65
GsuI CTGGAG 1 cut(s) 932
HaeIII GGCC 3 cut(s) 1048, 1162, 1497
Hin1II CATG 4 cut(s) 12, 455, 1484, 1495
HincII GTYRAC 2 cut(s) 269, 1341
HindII GTYRAC 2 cut(s) 269, 1341
HindIII AAGCTT 2 cut(s) 1019, 1326
HinfI GANTC 7 cut(s) 222, 284, 550, 601, 613, 646, 914
HpaI GTTAAC 1 cut(s) 1341
HphI GGTGA 3 cut(s) 407, 710, 1102
Hpy166II GTNNAC 4 cut(s) 269, 1141, 1341, 1582
Hpy188III TCNNGA 8 cut(s) 110, 260, 475, 742, 918, 949, 1391, 1703
Hpy8I GTNNAC 4 cut(s) 269, 1141, 1341, 1582
HpyAV CCTTC 2 cut(s) 881, 1520
HpyCH4III ACNGT 2 cut(s) 26, 1338
HpyCH4V TGCA 5 cut(s) 629, 679, 1025, 1063, 1716
HpyF10VI GCNNNNNNNGC 4 cut(s) 977, 1165, 1268, 1415
HpyF3I CTNAG 8 cut(s) 66, 79, 158, 511, 1164, 1256, 1658, 1704
Hsp92II CATG 4 cut(s) 12, 455, 1484, 1495
Ksp22I TGATCA 1 cut(s) 429
KspAI GTTAAC 1 cut(s) 1341
Kzo9I GATC 9 cut(s) 69, 256, 329, 367, 371, 429, 852, 943, 1287
LmnI GCTCC 3 cut(s) 960, 1038, 1268
Lsp1109I GCAGC 3 cut(s) 1088, 1239, 1442
LweI GCATC 4 cut(s) 616, 1072, 1270, 1484
MaeI CTAG 4 cut(s) 650, 804, 1185, 1643
MaeIII GTNAC 5 cut(s) 413, 497, 698, 1243, 1684
MalI GATC 9 cut(s) 71, 258, 331, 369, 373, 431, 854, 945, 1289
MbiI CCGCTC 1 cut(s) 1312
MboI GATC 9 cut(s) 69, 256, 329, 367, 371, 429, 852, 943, 1287
MflI RGATCY 2 cut(s) 852, 1287
MhlI GDGCHC 5 cut(s) 1121, 1170, 1264, 1273, 1515
MluCI AATT 6 cut(s) 104, 463, 737, 1349, 1503, 1625
MlyI GAGTC 1 cut(s) 216
MroXI GAANNNNTTC 1 cut(s) 891
MseI TTAA 7 cut(s) 218, 834, 902, 1155, 1172, 1229, 1340
MvnI CGCG 1 cut(s) 1214
MwoI GCNNNNNNNGC 4 cut(s) 977, 1165, 1268, 1415
NdeI CATATG 2 cut(s) 309, 1563
NdeII GATC 9 cut(s) 69, 256, 329, 367, 371, 429, 852, 943, 1287
NlaIII CATG 4 cut(s) 12, 455, 1484, 1495
NlaIV GGNNCC 2 cut(s) 854, 1308
NmuCI GTSAC 2 cut(s) 413, 698
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 6 cut(s) 284, 550, 601, 613, 646, 914
PflFI GACNNNGTC 1 cut(s) 225
PkrI GCNGC 4 cut(s) 1103, 1161, 1254, 1432
PleI GAGTC 1 cut(s) 216
PpsI GAGTC 1 cut(s) 216
PsiI TTATAA 1 cut(s) 1544
Psp124BI GAGCTC 3 cut(s) 1121, 1170, 1264
PspEI GGTNACC 1 cut(s) 413
PspFI CCCAGC 1 cut(s) 61
PspN4I GGNNCC 2 cut(s) 854, 1308
PspPI GGNCC 2 cut(s) 1046, 1495
PsuI RGATCY 2 cut(s) 852, 1287
PsyI GACNNNGTC 1 cut(s) 225
RsaI GTAC 2 cut(s) 1373, 1694
RsaNI GTAC 2 cut(s) 1372, 1693
SacI GAGCTC 3 cut(s) 1121, 1170, 1264
SaqAI TTAA 7 cut(s) 218, 834, 902, 1155, 1172, 1229, 1340
SatI GCNGC 4 cut(s) 1102, 1160, 1253, 1431
Sau3AI GATC 9 cut(s) 69, 256, 329, 367, 371, 429, 852, 943, 1287
Sau96I GGNCC 2 cut(s) 1046, 1495
SchI GAGTC 1 cut(s) 216
SduI GDGCHC 5 cut(s) 1121, 1170, 1264, 1273, 1515
SfaNI GCATC 4 cut(s) 616, 1072, 1270, 1484
SmlI CTYRAG 1 cut(s) 342
SmoI CTYRAG 1 cut(s) 342
Sse9I AATT 6 cut(s) 104, 463, 737, 1349, 1503, 1625
SsiI CCGC 5 cut(s) 786, 1159, 1214, 1298, 1310
SspI AATATT 1 cut(s) 675
SspMI CTAG 4 cut(s) 650, 804, 1185, 1643
SstI GAGCTC 3 cut(s) 1121, 1170, 1264
StyI CCWWGG 1 cut(s) 1236
TaaI ACNGT 2 cut(s) 26, 1338
TaqI TCGA 4 cut(s) 111, 370, 1421, 1437
TaqII GACCGA 1 cut(s) 215
TasI AATT 6 cut(s) 104, 463, 737, 1349, 1503, 1625
TauI GCSGC 1 cut(s) 1162
TfiI GAWTC 6 cut(s) 284, 550, 601, 613, 646, 914
Tru1I TTAA 7 cut(s) 218, 834, 902, 1155, 1172, 1229, 1340
Tru9I TTAA 7 cut(s) 218, 834, 902, 1155, 1172, 1229, 1340
TseFI GTSAC 2 cut(s) 413, 698
TseI GCWGC 3 cut(s) 1101, 1252, 1430
Tsp45I GTSAC 2 cut(s) 413, 698
TspDTI ATGAA 3 cut(s) 75, 414, 1539
TspGWI ACGGA 3 cut(s) 845, 947, 1215
Tth111I GACNNNGTC 1 cut(s) 225
XapI RAATTY 2 cut(s) 104, 1349
XcmI CCANNNNNNNNNTGG 1 cut(s) 307
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 4 cut(s) 650, 804, 1185, 1643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.