FvH4_7g00280

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0005122
Physical Location & Seq
Forward (+)
895734 .. 899836
4103 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g00280.t5

Sequence Viewer

Length: 1536 bp
ATGGCCAAGTCAAAAAGCACCAAGAACAAGGTCTCTTACATTTCAGTCCCTTCTCAGATTATCAACTCTCTCTCTGCATCTTCTCTGCAATCCCTCCTTACTTCACCCAAGAAATCTTCAAGGAGCAAAGCTAGGTACTTGAGCTTTCTCACCAAGTACAGGAGCCCAAGTTTCTGGGTTTTCTTCCTCTTCCTCTTTGGATTTCTCGGCATGCTCAAGCTCGGCTTCAATTTAAACACTTTGATCTCCTTTTCTCCTTACCCATGTACCACAACCACAACCCACTTCCAGGCCTTGGTCTCCTCAAATGGGTATTTGAAATCACACATGAGTATTGCTTCAAATGAAAATGGTCAGAAAGCTGAGGTCTTTGATAGTTTGACTCATTCACTCCCCCTGATTCTAAAGGAGGAGGAGGAGCCCAAACCAGAACCGAACAGTGATAAGACAGTAAGTGAGTTCTGGAAGCAACCAGATGGTTTGGGGTATAGGCCATGCTTGGATTTCACCCGGGACTATCAGAAGGCGAGTGAGGTGACAGTCAAGGACAGGACTAAATACCTGATGGTTGTGGTTGCTGGAGGGCTGAACCAGCAAAGGAACCAGATTGTTGATGCTGTTGTTATTGCTAGGATTCTTGGGGCTGCTTTGGTCGTTCCCATCTTGCAAGTCAATGTCATCTGGGGAGATGAAAGTGAATTTTCTGATATATTTGATATGGAGCACTTCAAGAAAGTTCTTGCTGATGATGTCCGTATAGTTTCTTCACTGCCATCAACACATATAATGACAAGGCCAGTGGAGGAGAAACGGACTCCTCTTCATGTTTCTCCTAGTTGGATTCGGTCACGCTATCTCAAGAGGATTAAAAGAGAAGGGGTTTTGCTTCTAAGAGGCTTGGATTCAAGGCTCTCTAAGGATCTTCCTTCTGATCTTCAGAAGCTTCGATGCAAGGTTGCTTTTCATGCATTGAGGTTTGCTCCTCCAATTTTGGGAATTGGCAACAAGCTTGCGGAGAGGATGCGTAGCAAGGGACCCTACCTTGCCCTTCATCTAAGAATGGAGAAGGATGTATGGGTAAGGACTGGTTGCCTTCCTGGTCTCAGTCATGAATACGATGAGATAGTAAACAGTGAAAGGATACAACGGCCTGAGCTCCTCACTGGACGATCAAACATGACTTACCACGAAAGAAAACTTGCTGGTCTCTGCCCTTTGAATTCCTTGGAAGTGACTAGGTTGCTAAAAGCTCTAGGAGCTCCTAAAGATGCAAGAATATATTGGGCTGGAGGGGAGCCACTGGGCGGAAAAGAAGCCTTGCTTCCATTAACCCGGGAATTTTCCCATTTTTACAACAAGGAAGATCTTGCTTTGCCTGGTGAGCTAGAACCTTTTGCAAAAAGAGCTTCTGTCATGGCTGCCATTGATTATATAGTTTCCGAGAACAGTGATGTCTTCATGCCGTCTCATGGGGGAAATATGGGTCATGCACTCCAGGTGGACAACGTGCCTATGCTGGCCACAAGAAGTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

512

Amino Acids

57.41

Weight (kDa)

9.41

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 187 - 499 3.9e-85 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 295
AciI CCGC 2 cut(s) 1013, 1305
AclWI GGATC 1 cut(s) 927
AcoI YGGCCR 2 cut(s) 3, 1518
AcsI RAATTY 3 cut(s) 698, 1219, 1337
AcuI CTGAAG 1 cut(s) 920
AfaI GTAC 3 cut(s) 137, 158, 268
AfiI CCNNNNNNNGG 7 cut(s) 159, 289, 295, 309, 992, 1304, 1469
AgsI TTSAA 7 cut(s) 120, 229, 319, 342, 730, 906, 1219
AjnI CCWGG 4 cut(s) 288, 1096, 1375, 1494
Alw21I GWGCWC 3 cut(s) 726, 1158, 1261
Alw26I GTCTC 5 cut(s) 37, 304, 1106, 1211, 1470
AlwI GGATC 1 cut(s) 927
Ama87I CYCGRG 2 cut(s) 510, 1332
AoxI GGCC 6 cut(s) 3, 291, 491, 794, 1148, 1518
ApeKI GCWGC 2 cut(s) 644, 1418
ApoI RAATTY 3 cut(s) 698, 1219, 1337
AspS9I GGNCC 1 cut(s) 1034
AsuC2I CCSGG 4 cut(s) 511, 512, 1333, 1334
AsuHPI GGTGA 5 cut(s) 96, 142, 499, 547, 1391
AvaI CYCGRG 2 cut(s) 510, 1332
AvaII GGWCC 1 cut(s) 1034
BaeI ACNNNNGTAYC 2 cut(s) 250, 283
BalI TGGCCA 2 cut(s) 5, 1520
BanII GRGCYC 4 cut(s) 167, 423, 1158, 1261
BarI GAAGNNNNNNTAC 2 cut(s) 748, 780
BbsI GAAGAC 1 cut(s) 1447
Bbv12I GWGCWC 3 cut(s) 726, 1158, 1261
BbvCI CCTCAGC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 631, 1405
BccI CCATC 4 cut(s) 470, 559, 668, 781
BceAI ACGGC 2 cut(s) 1163, 1447
BciT130I CCWGG 4 cut(s) 290, 1098, 1377, 1496
BciVI GTATCC 1 cut(s) 1134
BcnI CCSGG 4 cut(s) 511, 512, 1333, 1334
BcoDI GTCTC 5 cut(s) 37, 304, 1106, 1211, 1470
BfaI CTAG 6 cut(s) 132, 630, 834, 1236, 1253, 1385
BfuI GTATCC 1 cut(s) 1134
BglII AGATCT 1 cut(s) 1363
BisI GCNGC 2 cut(s) 645, 1419
BlsI GCNGC 2 cut(s) 646, 1420
Bme1390I CCNGG 8 cut(s) 290, 511, 512, 1098, 1333, 1334, 1377, 1496
Bme18I GGWCC 1 cut(s) 1034
BmeT110I CYCGRG 2 cut(s) 510, 1332
BmgT120I GGNCC 1 cut(s) 1034
BmiI GGNNCC 6 cut(s) 164, 420, 602, 1035, 1036, 1296
BmrFI CCNGG 8 cut(s) 290, 511, 512, 1098, 1333, 1334, 1377, 1496
BmrI ACTGGG 1 cut(s) 1310
BmsI GCATC 5 cut(s) 86, 604, 938, 1011, 1258
BmuI ACTGGG 1 cut(s) 1310
BpiI GAAGAC 1 cut(s) 1447
BplI GAGNNNNNCTC 2 cut(s) 964, 996
BpmI CTGGAG 3 cut(s) 600, 1308, 1478
Bpu10I CCTNAGC 2 cut(s) 363, 1152
BpuEI CTTGAG 3 cut(s) 160, 200, 842
BpuMI CCSGG 4 cut(s) 511, 512, 1333, 1334
BsaI GGTCTC 4 cut(s) 37, 304, 1106, 1211
BsaJI CCNNGG 4 cut(s) 294, 510, 1224, 1332
BsaXI ACNNNNNCTCC 2 cut(s) 154, 184
Bsc4I CCNNNNNNNGG 7 cut(s) 159, 289, 295, 309, 992, 1304, 1469
Bse1I ACTGG 4 cut(s) 797, 1090, 1168, 1305
BseBI CCWGG 4 cut(s) 290, 1098, 1377, 1496
BseDI CCNNGG 4 cut(s) 294, 510, 1224, 1332
BseGI GGATG 2 cut(s) 1026, 1075
BseLI CCNNNNNNNGG 7 cut(s) 159, 289, 295, 309, 992, 1304, 1469
BseMII CTCAG 4 cut(s) 68, 354, 1117, 1143
BseNI ACTGG 4 cut(s) 797, 1090, 1168, 1305
BseRI GAGGAG 8 cut(s) 292, 425, 428, 431, 807, 818, 972, 1148
BseXI GCAGC 2 cut(s) 631, 1405
BshFI GGCC 6 cut(s) 5, 293, 493, 796, 1150, 1520
BsiHKAI GWGCWC 3 cut(s) 726, 1158, 1261
BsiHKCI CYCGRG 2 cut(s) 510, 1332
BsiSI CCGG 2 cut(s) 511, 1333
BslFI GGGAC 3 cut(s) 32, 527, 1047
BslI CCNNNNNNNGG 7 cut(s) 159, 289, 295, 309, 992, 1304, 1469
BsmAI GTCTC 5 cut(s) 37, 304, 1106, 1211, 1470
BsmBI CGTCTC 1 cut(s) 1470
BsmFI GGGAC 3 cut(s) 32, 527, 1047
BsnI GGCC 6 cut(s) 5, 293, 493, 796, 1150, 1520
Bso31I GGTCTC 4 cut(s) 37, 304, 1106, 1211
BsoBI CYCGRG 2 cut(s) 510, 1332
Bsp1286I GDGCHC 5 cut(s) 167, 423, 726, 1158, 1261
Bsp143I GATC 5 cut(s) 243, 919, 931, 1169, 1363
BspACI CCGC 2 cut(s) 1013, 1305
BspANI GGCC 6 cut(s) 5, 293, 493, 796, 1150, 1520
BspCNI CTCAG 4 cut(s) 67, 355, 1116, 1144
BspHI TCATGA 1 cut(s) 1108
BspLI GGNNCC 6 cut(s) 164, 420, 602, 1035, 1036, 1296
BspPI GGATC 1 cut(s) 927
BspTNI GGTCTC 4 cut(s) 37, 304, 1106, 1211
BsrI ACTGG 4 cut(s) 797, 1090, 1168, 1305
BssECI CCNNGG 4 cut(s) 294, 510, 1224, 1332
BssMI GATC 5 cut(s) 243, 919, 931, 1169, 1363
BssT1I CCWWGG 2 cut(s) 294, 1224
Bst2UI CCWGG 4 cut(s) 290, 1098, 1377, 1496
Bst4CI ACNGT 5 cut(s) 440, 451, 541, 1133, 1448
Bst6I CTCTTC 2 cut(s) 194, 825
BstC8I GCNNGC 3 cut(s) 212, 1011, 1518
BstDEI CTNAG 7 cut(s) 54, 363, 890, 915, 1055, 1103, 1152
BstF5I GGATG 2 cut(s) 1026, 1075
BstKTI GATC 5 cut(s) 246, 922, 934, 1172, 1366
BstMAI GTCTC 5 cut(s) 37, 304, 1106, 1211, 1470
BstMBI GATC 5 cut(s) 243, 919, 931, 1169, 1363
BstMWI GCNNNNNNNGC 5 cut(s) 592, 965, 1256, 1381, 1403
BstNI CCWGG 4 cut(s) 290, 1098, 1377, 1496
BstNSI RCATGY 1 cut(s) 214
BstSCI CCNGG 8 cut(s) 288, 509, 510, 1096, 1331, 1332, 1375, 1494
BstV1I GCAGC 2 cut(s) 631, 1405
BstV2I GAAGAC 1 cut(s) 1447
BstX2I RGATCY 2 cut(s) 919, 1363
BstXI CCANNNNNNTGG 1 cut(s) 174
BstYI RGATCY 2 cut(s) 919, 1363
BsuI GTATCC 1 cut(s) 1134
BsuRI GGCC 6 cut(s) 5, 293, 493, 796, 1150, 1520
BtsCI GGATG 2 cut(s) 1026, 1075
BtsI GCAGTG 1 cut(s) 767
BtsIMutI CAGTG 7 cut(s) 445, 767, 804, 1138, 1161, 1298, 1453
Cac8I GCNNGC 3 cut(s) 212, 1011, 1518
CciI TCATGA 1 cut(s) 1108
Cfr13I GGNCC 1 cut(s) 1034
Cfr9I CCCGGG 2 cut(s) 510, 1332
Csp6I GTAC 3 cut(s) 136, 157, 267
CspCI CAANNNNNGTGG 2 cut(s) 780, 815
CviQI GTAC 3 cut(s) 136, 157, 267
DdeI CTNAG 7 cut(s) 54, 363, 890, 915, 1055, 1103, 1152
DpnI GATC 5 cut(s) 245, 921, 933, 1171, 1365
DpnII GATC 5 cut(s) 243, 919, 931, 1169, 1363
DraI TTTAAA 1 cut(s) 234
EaeI YGGCCR 2 cut(s) 3, 1518
Eam1104I CTCTTC 2 cut(s) 194, 825
EarI CTCTTC 2 cut(s) 194, 825
EciI GGCGGA 1 cut(s) 1320
Ecl136II GAGCTC 2 cut(s) 1156, 1259
Eco130I CCWWGG 2 cut(s) 294, 1224
Eco147I AGGCCT 1 cut(s) 293
Eco24I GRGCYC 4 cut(s) 167, 423, 1158, 1261
Eco31I GGTCTC 4 cut(s) 37, 304, 1106, 1211
Eco47I GGWCC 1 cut(s) 1034
Eco53kI GAGCTC 2 cut(s) 1156, 1259
Eco57I CTGAAG 1 cut(s) 920
Eco88I CYCGRG 2 cut(s) 510, 1332
EcoICRI GAGCTC 2 cut(s) 1156, 1259
EcoO109I RGGNCCY 1 cut(s) 1034
EcoRI GAATTC 1 cut(s) 1219
EcoRII CCWGG 4 cut(s) 288, 1096, 1375, 1494
EcoT14I CCWWGG 2 cut(s) 294, 1224
EcoT22I ATGCAT 1 cut(s) 970
EcoT38I GRGCYC 4 cut(s) 167, 423, 1158, 1261
ErhI CCWWGG 2 cut(s) 294, 1224
Esp3I CGTCTC 1 cut(s) 1470
FalI AAGNNNNNCTT 4 cut(s) 209, 241, 1305, 1337
FaqI GGGAC 3 cut(s) 32, 527, 1047
Fnu4HI GCNGC 2 cut(s) 645, 1419
FokI GGATG 2 cut(s) 1033, 1082
FriOI GRGCYC 4 cut(s) 167, 423, 1158, 1261
Fsp4HI GCNGC 2 cut(s) 645, 1419
FspBI CTAG 6 cut(s) 132, 630, 834, 1236, 1253, 1385
GluI GCNGC 2 cut(s) 645, 1419
GsuI CTGGAG 3 cut(s) 600, 1308, 1478
HaeIII GGCC 6 cut(s) 5, 293, 493, 796, 1150, 1520
HapII CCGG 2 cut(s) 511, 1333
HindIII AAGCTT 2 cut(s) 941, 1007
HinfI GANTC 6 cut(s) 382, 400, 634, 814, 841, 902
HpaII CCGG 2 cut(s) 511, 1333
HphI GGTGA 5 cut(s) 96, 142, 499, 547, 1391
Hpy166II GTNNAC 2 cut(s) 1129, 1501
Hpy188I TCNGA 7 cut(s) 57, 357, 522, 706, 931, 939, 1441
Hpy188III TCNNGA 4 cut(s) 463, 730, 859, 1109
Hpy8I GTNNAC 2 cut(s) 1129, 1501
HpyAV CCTTC 7 cut(s) 60, 517, 869, 936, 1058, 1060, 1103
HpyCH4III ACNGT 5 cut(s) 440, 451, 541, 1133, 1448
HpyCH4IV ACGT 1 cut(s) 1506
HpyCH4V TGCA 8 cut(s) 77, 88, 667, 951, 968, 1271, 1397, 1490
HpyF10VI GCNNNNNNNGC 5 cut(s) 592, 965, 1256, 1381, 1403
HpyF3I CTNAG 7 cut(s) 54, 363, 890, 915, 1055, 1103, 1152
HpySE526I ACGT 1 cut(s) 1506
KflI GGGWCCC 1 cut(s) 1034
Kzo9I GATC 5 cut(s) 243, 919, 931, 1169, 1363
LmnI GCTCC 9 cut(s) 123, 162, 418, 721, 985, 1161, 1256, 1264, 1294
Lsp1109I GCAGC 2 cut(s) 631, 1405
LweI GCATC 5 cut(s) 86, 604, 938, 1011, 1258
MaeI CTAG 6 cut(s) 132, 630, 834, 1236, 1253, 1385
MaeII ACGT 1 cut(s) 1506
MaeIII GTNAC 3 cut(s) 535, 846, 1231
MalI GATC 5 cut(s) 245, 921, 933, 1171, 1365
MboI GATC 5 cut(s) 243, 919, 931, 1169, 1363
MflI RGATCY 2 cut(s) 919, 1363
MhlI GDGCHC 5 cut(s) 167, 423, 726, 1158, 1261
MlsI TGGCCA 2 cut(s) 5, 1520
MluCI AATT 6 cut(s) 229, 698, 987, 996, 1219, 1337
MluNI TGGCCA 2 cut(s) 5, 1520
MlyI GAGTC 2 cut(s) 376, 808
MmeI TCCRAC 1 cut(s) 818
Mox20I TGGCCA 2 cut(s) 5, 1520
Mph1103I ATGCAT 1 cut(s) 970
MscI TGGCCA 2 cut(s) 5, 1520
MseI TTAA 3 cut(s) 233, 867, 1328
Msp20I TGGCCA 2 cut(s) 5, 1520
MspI CCGG 2 cut(s) 511, 1333
MspR9I CCNGG 8 cut(s) 290, 511, 512, 1098, 1333, 1334, 1377, 1496
MvaI CCWGG 4 cut(s) 290, 1098, 1377, 1496
MwoI GCNNNNNNNGC 5 cut(s) 592, 965, 1256, 1381, 1403
NciI CCSGG 4 cut(s) 511, 512, 1333, 1334
NdeII GATC 5 cut(s) 243, 919, 931, 1169, 1363
NlaIV GGNNCC 6 cut(s) 164, 420, 602, 1035, 1036, 1296
NmeAIII GCCGAG 2 cut(s) 186, 201
NmuCI GTSAC 3 cut(s) 535, 846, 1231
NsiI ATGCAT 1 cut(s) 970
NspI RCATGY 1 cut(s) 214
PaeI GCATGC 1 cut(s) 214
PagI TCATGA 1 cut(s) 1108
PceI AGGCCT 1 cut(s) 293
PfeI GAWTC 4 cut(s) 400, 634, 841, 902
PflMI CCANNNNNTGG 1 cut(s) 295
PkrI GCNGC 2 cut(s) 646, 1420
PleI GAGTC 2 cut(s) 376, 808
PpsI GAGTC 2 cut(s) 376, 808
PpuMI RGGWCCY 1 cut(s) 1034
Psp124BI GAGCTC 2 cut(s) 1158, 1261
Psp5II RGGWCCY 1 cut(s) 1034
Psp6I CCWGG 4 cut(s) 288, 1096, 1375, 1494
PspGI CCWGG 4 cut(s) 288, 1096, 1375, 1494
PspN4I GGNNCC 6 cut(s) 164, 420, 602, 1035, 1036, 1296
PspPI GGNCC 1 cut(s) 1034
PspPPI RGGWCCY 1 cut(s) 1034
PsuI RGATCY 2 cut(s) 919, 1363
RsaI GTAC 3 cut(s) 137, 158, 268
RsaNI GTAC 3 cut(s) 136, 157, 267
SacI GAGCTC 2 cut(s) 1158, 1261
SaqAI TTAA 3 cut(s) 233, 867, 1328
SatI GCNGC 2 cut(s) 645, 1419
Sau3AI GATC 5 cut(s) 243, 919, 931, 1169, 1363
Sau96I GGNCC 1 cut(s) 1034
SchI GAGTC 2 cut(s) 376, 808
ScrFI CCNGG 8 cut(s) 290, 511, 512, 1098, 1333, 1334, 1377, 1496
SduI GDGCHC 5 cut(s) 167, 423, 726, 1158, 1261
SfaNI GCATC 5 cut(s) 86, 604, 938, 1011, 1258
SinI GGWCC 1 cut(s) 1034
SmaI CCCGGG 2 cut(s) 512, 1334
SmlI CTYRAG 3 cut(s) 139, 215, 857
SmoI CTYRAG 3 cut(s) 139, 215, 857
SphI GCATGC 1 cut(s) 214
Sse9I AATT 6 cut(s) 229, 698, 987, 996, 1219, 1337
SseBI AGGCCT 1 cut(s) 293
SsiI CCGC 2 cut(s) 1013, 1305
SspMI CTAG 6 cut(s) 132, 630, 834, 1236, 1253, 1385
SstI GAGCTC 2 cut(s) 1158, 1261
StuI AGGCCT 1 cut(s) 293
StyD4I CCNGG 8 cut(s) 288, 509, 510, 1096, 1331, 1332, 1375, 1494
StyI CCWWGG 2 cut(s) 294, 1224
TaaI ACNGT 5 cut(s) 440, 451, 541, 1133, 1448
TaiI ACGT 1 cut(s) 1509
TaqI TCGA 1 cut(s) 946
TaqII GACCGA 1 cut(s) 834
TasI AATT 6 cut(s) 229, 698, 987, 996, 1219, 1337
TatI WGTACW 1 cut(s) 156
TfiI GAWTC 4 cut(s) 400, 634, 841, 902
Tru1I TTAA 3 cut(s) 233, 867, 1328
Tru9I TTAA 3 cut(s) 233, 867, 1328
TscAI CASTG 7 cut(s) 445, 774, 804, 1138, 1168, 1305, 1453
TseFI GTSAC 3 cut(s) 535, 846, 1231
TseI GCWGC 2 cut(s) 644, 1418
Tsp45I GTSAC 3 cut(s) 535, 846, 1231
TspDTI ATGAA 7 cut(s) 360, 705, 812, 953, 1040, 1125, 1447
TspGWI ACGGA 2 cut(s) 743, 826
TspMI CCCGGG 2 cut(s) 510, 1332
TspRI CASTG 7 cut(s) 445, 774, 804, 1138, 1168, 1305, 1453
Van91I CCANNNNNTGG 1 cut(s) 295
VpaK11BI GGWCC 1 cut(s) 1034
XapI RAATTY 3 cut(s) 698, 1219, 1337
XceI RCATGY 1 cut(s) 214
XmaI CCCGGG 2 cut(s) 510, 1332
XspI CTAG 6 cut(s) 132, 630, 834, 1236, 1253, 1385
Zsp2I ATGCAT 1 cut(s) 970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.