AT2G44578

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0019143
Physical Location & Seq
Reverse (-)
18400646 .. 18401339
694 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44578.1

Sequence Viewer

Length: 438 bp
ATGTTTATTGAAACTGTGATTAGATTCTCTCAGATCATCGCCGAATCCGTCATCATCGCCTCCGTCGTCGTCTTGACTCTCCGTCTCTTCTTCAGATTCGCTTGTTTCCTCGCGAGCCGTTCTTGGCGTCGTTACCGTACATTCACGGTCCAACATCGCCGGAGATGGAGAAAAACCGCGGCGGAGGAAAAACATTCATCACCGTATTGTACGATTTGTCTCGAAGATGCGGCGGAGGGAGAGAAGATGAGGCGGATAACGGCGTGCAGTCACTGTTTCCACGTCGATTGCATCGATCCGTGGTTGATGAAGAAGTCCACGTGTCCATTGTGCAGAGCTGAGATACCGCCGGTGCCGCCGGGAAATCCTCTGGTGGCGCTTTTTGTTCCTCCCGGCGTGATTGAGATGTTCACCAAAGGAATCATCCCCGACGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

145

Amino Acids

16.64

Weight (kDa)

9.2

Isoelectric Point (pI)

69.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_2 PF13923 69 - 111 4.2e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 70 - 112 8.7e-14 Ring finger domain
zf-RING_11 PF17123 70 - 98 2.9e-09 RING-like zinc finger
zf-C3HC4 PF00097 70 - 111 2e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 71 - 112 6.4e-10 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44578 AT2G44581 AT3G60966
malus_domestica MD01G1155300.v1.1 MD07G1086600.v1.1
prunus_persica Prupe.2G113400_v2.0.a1 Prupe.2G252900_v2.0.a1
pyrus_communis pycom01g17260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 352
AccII CGCG 3 cut(s) 113, 179, 434
AciI CCGC 8 cut(s) 177, 179, 182, 230, 233, 253, 347, 356
AclWI GGATC 1 cut(s) 290
AcuI CTGAAG 1 cut(s) 76
AcvI CACGTG 1 cut(s) 321
AcyI GRCGYC 1 cut(s) 127
AfaI GTAC 2 cut(s) 139, 211
AflIII ACRYGT 2 cut(s) 320, 432
AgsI TTSAA 1 cut(s) 11
AhdI GACNNNNNGTC 1 cut(s) 81
AjiI CACGTC 1 cut(s) 283
AluBI AGCT 1 cut(s) 338
AluI AGCT 1 cut(s) 338
Alw26I GTCTC 2 cut(s) 89, 224
AlwI GGATC 1 cut(s) 290
AlwNI CAGNNNCTG 1 cut(s) 273
AspLEI GCGC 1 cut(s) 379
AspS9I GGNCC 1 cut(s) 148
AsuC2I CCSGG 2 cut(s) 360, 393
AsuHPI GGTGA 2 cut(s) 192, 403
AvaII GGWCC 1 cut(s) 148
BanI GGYRCC 1 cut(s) 352
BbrPI CACGTG 1 cut(s) 321
BccI CCATC 1 cut(s) 159
BceAI ACGGC 2 cut(s) 102, 276
BcnI CCSGG 2 cut(s) 360, 393
BcoDI GTCTC 2 cut(s) 89, 224
BfoI RGCGCY 1 cut(s) 380
BisI GCNGC 3 cut(s) 180, 231, 356
BlsI GCNGC 3 cut(s) 181, 232, 357
Bme1390I CCNGG 2 cut(s) 360, 393
Bme18I GGWCC 1 cut(s) 148
BmeRI GACNNNNNGTC 1 cut(s) 81
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 1 cut(s) 148
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 2 cut(s) 360, 393
BmsI GCATC 2 cut(s) 217, 300
BpuMI CCSGG 2 cut(s) 360, 393
Bsa29I ATCGAT 1 cut(s) 294
BsaAI YACGTR 1 cut(s) 321
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 177, 299
Bse118I RCCGGY 1 cut(s) 349
BseCI ATCGAT 1 cut(s) 294
BseDI CCNNGG 2 cut(s) 177, 299
BseGI GGATG 1 cut(s) 423
BseMII CTCAG 2 cut(s) 44, 330
BsgI GTGCAG 2 cut(s) 286, 352
Bsh1236I CGCG 3 cut(s) 113, 179, 434
BshNI GGYRCC 1 cut(s) 352
BshVI ATCGAT 1 cut(s) 294
BsiSI CCGG 4 cut(s) 160, 350, 359, 393
BsmAI GTCTC 2 cut(s) 89, 224
BsmBI CGTCTC 1 cut(s) 89
Bsp143I GATC 2 cut(s) 33, 295
Bsp68I TCGCGA 1 cut(s) 113
BspACI CCGC 8 cut(s) 177, 179, 182, 230, 233, 253, 347, 356
BspCNI CTCAG 2 cut(s) 43, 331
BspDI ATCGAT 1 cut(s) 294
BspFNI CGCG 3 cut(s) 113, 179, 434
BspLI GGNNCC 1 cut(s) 354
BspPI GGATC 1 cut(s) 290
BspT107I GGYRCC 1 cut(s) 352
BsrFI RCCGGY 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 177, 299
BssMI GATC 2 cut(s) 33, 295
BssNI GRCGYC 1 cut(s) 127
Bst4CI ACNGT 5 cut(s) 16, 137, 148, 204, 275
Bst6I CTCTTC 1 cut(s) 92
BstACI GRCGYC 1 cut(s) 127
BstBAI YACGTR 1 cut(s) 321
BstC8I GCNNGC 2 cut(s) 115, 265
BstDEI CTNAG 2 cut(s) 30, 339
BstDSI CCRYGG 2 cut(s) 177, 299
BstF5I GGATG 1 cut(s) 423
BstFNI CGCG 3 cut(s) 113, 179, 434
BstH2I RGCGCY 1 cut(s) 380
BstHHI GCGC 1 cut(s) 379
BstKTI GATC 2 cut(s) 36, 298
BstMAI GTCTC 2 cut(s) 89, 224
BstMBI GATC 2 cut(s) 33, 295
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstSCI CCNGG 2 cut(s) 358, 391
BstUI CGCG 3 cut(s) 113, 179, 434
Bsu15I ATCGAT 1 cut(s) 294
BsuTUI ATCGAT 1 cut(s) 294
BtgI CCRYGG 2 cut(s) 177, 299
BtgZI GCGATG 3 cut(s) 22, 40, 140
BtrI CACGTC 1 cut(s) 283
BtsCI GGATG 1 cut(s) 423
BtsIMutI CAGTG 1 cut(s) 271
BtuMI TCGCGA 1 cut(s) 113
Cac8I GCNNGC 2 cut(s) 115, 265
CaiI CAGNNNCTG 1 cut(s) 273
CfoI GCGC 1 cut(s) 379
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 1 cut(s) 148
Cfr42I CCGCGG 1 cut(s) 180
ClaI ATCGAT 1 cut(s) 294
CseI GACGC 1 cut(s) 116
Csp6I GTAC 2 cut(s) 138, 210
CspCI CAANNNNNGTGG 2 cut(s) 269, 304
CviJI RGCY 2 cut(s) 117, 338
CviKI_1 RGCY 2 cut(s) 117, 338
CviQI GTAC 2 cut(s) 138, 210
DdeI CTNAG 2 cut(s) 30, 339
DpnI GATC 2 cut(s) 35, 297
DpnII GATC 2 cut(s) 33, 295
DriI GACNNNNNGTC 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 92
Eam1105I GACNNNNNGTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 92
EciI GGCGGA 3 cut(s) 197, 248, 268
Eco47I GGWCC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 76
Eco72I CACGTG 1 cut(s) 321
Esp3I CGTCTC 1 cut(s) 89
Fnu4HI GCNGC 3 cut(s) 180, 231, 356
FokI GGATG 1 cut(s) 410
Fsp4HI GCNGC 3 cut(s) 180, 231, 356
GlaI GCGC 1 cut(s) 378
GluI GCNGC 3 cut(s) 180, 231, 356
HaeII RGCGCY 1 cut(s) 380
HapII CCGG 4 cut(s) 160, 350, 359, 393
HgaI GACGC 1 cut(s) 116
HhaI GCGC 1 cut(s) 379
Hin1I GRCGYC 1 cut(s) 127
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HinfI GANTC 5 cut(s) 24, 44, 76, 96, 420
HpaII CCGG 4 cut(s) 160, 350, 359, 393
HphI GGTGA 2 cut(s) 192, 403
Hpy166II GTNNAC 2 cut(s) 318, 411
Hpy188I TCNGA 2 cut(s) 33, 95
Hpy188III TCNNGA 3 cut(s) 73, 112, 221
Hpy8I GTNNAC 2 cut(s) 318, 411
Hpy99I CGWCG 5 cut(s) 68, 71, 132, 287, 434
HpyCH4III ACNGT 5 cut(s) 16, 137, 148, 204, 275
HpyCH4IV ACGT 2 cut(s) 282, 320
HpyCH4V TGCA 3 cut(s) 267, 291, 333
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpyF3I CTNAG 2 cut(s) 30, 339
HpySE526I ACGT 2 cut(s) 282, 320
Hsp92I GRCGYC 1 cut(s) 127
HspAI GCGC 1 cut(s) 377
KspI CCGCGG 1 cut(s) 180
Kzo9I GATC 2 cut(s) 33, 295
LpnPI CCDG 5 cut(s) 173, 356, 363, 372, 406
LweI GCATC 2 cut(s) 217, 300
MaeII ACGT 2 cut(s) 282, 320
MaeIII GTNAC 2 cut(s) 131, 269
MalI GATC 2 cut(s) 35, 297
MboI GATC 2 cut(s) 33, 295
MboII GAAGA 5 cut(s) 79, 82, 236, 256, 322
MluI ACGCGT 1 cut(s) 432
MlyI GAGTC 1 cut(s) 70
MmeI TCCRAC 1 cut(s) 175
MnlI CCTC 7 cut(s) 70, 119, 178, 229, 243, 378, 399
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 4 cut(s) 160, 350, 359, 393
MspR9I CCNGG 2 cut(s) 360, 393
MvnI CGCG 3 cut(s) 113, 179, 434
MwoI GCNNNNNNNGC 1 cut(s) 355
NciI CCSGG 2 cut(s) 360, 393
NdeII GATC 2 cut(s) 33, 295
NlaIV GGNNCC 1 cut(s) 354
NmuCI GTSAC 1 cut(s) 269
NruI TCGCGA 1 cut(s) 113
PcsI WCGNNNNNNNCGW 3 cut(s) 45, 63, 291
PfeI GAWTC 4 cut(s) 24, 44, 96, 420
PkrI GCNGC 3 cut(s) 181, 232, 357
PleI GAGTC 1 cut(s) 70
PmaCI CACGTG 1 cut(s) 321
PmlI CACGTG 1 cut(s) 321
PpsI GAGTC 1 cut(s) 70
Ppu21I YACGTR 1 cut(s) 321
PspCI CACGTG 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 354
PspPI GGNCC 1 cut(s) 148
PstNI CAGNNNCTG 1 cut(s) 273
RruI TCGCGA 1 cut(s) 113
RsaI GTAC 2 cut(s) 139, 211
RsaNI GTAC 2 cut(s) 138, 210
SacII CCGCGG 1 cut(s) 180
SatI GCNGC 3 cut(s) 180, 231, 356
Sau3AI GATC 2 cut(s) 33, 295
Sau96I GGNCC 1 cut(s) 148
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 2 cut(s) 360, 393
SetI ASST 3 cut(s) 285, 323, 340
SfaNI GCATC 2 cut(s) 217, 300
Sfr303I CCGCGG 1 cut(s) 180
SgrAI CRCCGGYG 1 cut(s) 349
SgrBI CCGCGG 1 cut(s) 180
SinI GGWCC 1 cut(s) 148
SsiI CCGC 8 cut(s) 177, 179, 182, 230, 233, 253, 347, 356
StyD4I CCNGG 2 cut(s) 358, 391
TaaI ACNGT 5 cut(s) 16, 137, 148, 204, 275
TaiI ACGT 2 cut(s) 285, 323
TaqI TCGA 3 cut(s) 222, 285, 294
TauI GCSGC 3 cut(s) 182, 233, 358
TfiI GAWTC 4 cut(s) 24, 44, 96, 420
TscAI CASTG 1 cut(s) 278
TseFI GTSAC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 2 cut(s) 186, 323
TspGWI ACGGA 4 cut(s) 37, 52, 71, 288
TspRI CASTG 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.