Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0019143

Physical Location & Seq

Genomic Coordinates Reverse (-)

26325496 .. 26325963

Gene Structure 468 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1155300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 468 bp

ATGACCTCCCAAGACAATCAGCCGTTAAACGTCCACTTCACCGTCCTTGACGACGATCAGTTCCGCGTCCGAGGCAAGACGCTGTTCGTCGTCGTCGTAATCTTCACCGTCGTCCTCCTCGTCACACTGTTCCTGCTCTACGCGCGCTGGCTCTGCCGTTTCCACCTCCACGACGTCTCCTCCGACGCGCCCCCCGCGCCGCCGCTGAATCGGCCCGAGGGCCTTGACGCCGCCTTCATCCGCGCCTTGCCGATTGTGTTGTACCAGGAATCGAACCGGGATTTGGAAGCCGGCAGCGGTGGCGAGTGCTGCATATGCCTGGGCGTGTTCGAAGACGGCGAGAAGGTGAAGGTTCTGCCGAACTGCCGCCATAGTTACCACTCTGAGTGTGTGGACACGTGGCTTTCGACCCATTCGAGCTGCCCAATCTGTCGCGCTCAGCCCCAAGCTCATTCCACTGCTTCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

156

Amino Acids

17.28

Weight (kDa)

5.73

Isoelectric Point (pI)

44.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	102 - 145	1.2e-14	Ring finger domain
zf-RING_11	PF17123	102 - 131	4.6e-10	RING-like zinc finger
zf-C3HC4_2	PF13923	102 - 144	7.8e-09	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	103 - 145	1.6e-07	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0019143)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44578 AT2G44581 AT3G60966
malus_domestica	MD01G1155300.v1.1 MD07G1086600.v1.1
prunus_persica	Prupe.2G113400_v2.0.a1 Prupe.2G252900_v2.0.a1
pyrus_communis	pycom01g17260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	177
AccII	CGCG	7 cut(s)	66, 143, 145, 188, 197, 243, 435
AciI	CCGC	8 cut(s)	64, 195, 200, 203, 231, 241, 297, 367
AcvI	CACGTG	1 cut(s)	399
AcyI	GRCGYC	2 cut(s)	174, 228
AfaI	GTAC	1 cut(s)	263
AfiI	CCNNNNNNNGG	1 cut(s)	283
AflIII	ACRYGT	1 cut(s)	396
AjnI	CCWGG	2 cut(s)	264, 318
AjuI	GAANNNNNNNTTGG	2 cut(s)	266, 298
AluBI	AGCT	2 cut(s)	420, 449
AluI	AGCT	2 cut(s)	420, 449
Alw26I	GTCTC	1 cut(s)	181
Ama87I	CYCGRG	1 cut(s)	215
AoxI	GGCC	2 cut(s)	212, 220
ApeKI	GCWGC	3 cut(s)	294, 309, 420
AspLEI	GCGC	6 cut(s)	145, 147, 190, 199, 245, 437
AspS9I	GGNCC	2 cut(s)	213, 220
AsuC2I	CCSGG	1 cut(s)	278
AsuHPI	GGTGA	3 cut(s)	31, 97, 358
AsuII	TTCGAA	1 cut(s)	330
AvaI	CYCGRG	1 cut(s)	215
BbrPI	CACGTG	1 cut(s)	399
BbsI	GAAGAC	1 cut(s)	339
BbvI	GCAGC	3 cut(s)	296, 306, 407
BceAI	ACGGC	3 cut(s)	7, 141, 352
BciT130I	CCWGG	2 cut(s)	266, 320
BcnI	CCSGG	1 cut(s)	278
BcoDI	GTCTC	1 cut(s)	181
BisI	GCNGC	7 cut(s)	200, 203, 231, 295, 310, 367, 421
BlpI	GCTNAGC	1 cut(s)	438
BlsI	GCNGC	7 cut(s)	201, 204, 232, 296, 311, 368, 422
Bme1390I	CCNGG	3 cut(s)	266, 278, 320
BmeT110I	CYCGRG	1 cut(s)	215
BmgT120I	GGNCC	2 cut(s)	213, 220
BmrFI	CCNGG	3 cut(s)	266, 278, 320
BpiI	GAAGAC	1 cut(s)	339
Bpu1102I	GCTNAGC	1 cut(s)	438
Bpu14I	TTCGAA	1 cut(s)	330
BpuMI	CCSGG	1 cut(s)	278
BsaAI	YACGTR	1 cut(s)	399
BsaHI	GRCGYC	2 cut(s)	174, 228
BsaJI	CCNNGG	3 cut(s)	70, 216, 319
BsaXI	ACNNNNNCTCC	4 cut(s)	161, 164, 191, 194
Bsc4I	CCNNNNNNNGG	1 cut(s)	283
Bse118I	RCCGGY	1 cut(s)	290
BseBI	CCWGG	2 cut(s)	266, 320
BseDI	CCNNGG	3 cut(s)	70, 216, 319
BseGI	GGATG	1 cut(s)	237
BseLI	CCNNNNNNNGG	1 cut(s)	283
BseMII	CTCAG	2 cut(s)	375, 452
BsePI	GCGCGC	1 cut(s)	143
BseRI	GAGGAG	2 cut(s)	107, 169
BseXI	GCAGC	3 cut(s)	296, 306, 407
Bsh1236I	CGCG	7 cut(s)	66, 143, 145, 188, 197, 243, 435
BshFI	GGCC	2 cut(s)	214, 222
BsiHKCI	CYCGRG	1 cut(s)	215
BsiSI	CCGG	2 cut(s)	277, 291
BslI	CCNNNNNNNGG	1 cut(s)	283
BsmAI	GTCTC	1 cut(s)	181
BsmBI	CGTCTC	1 cut(s)	181
BsnI	GGCC	2 cut(s)	214, 222
BsoBI	CYCGRG	1 cut(s)	215
Bsp119I	TTCGAA	1 cut(s)	330
Bsp143I	GATC	1 cut(s)	55
Bsp1720I	GCTNAGC	1 cut(s)	438
BspACI	CCGC	8 cut(s)	64, 195, 200, 203, 231, 241, 297, 367
BspANI	GGCC	2 cut(s)	214, 222
BspCNI	CTCAG	2 cut(s)	376, 451
BspFNI	CGCG	7 cut(s)	66, 143, 145, 188, 197, 243, 435
BspT104I	TTCGAA	1 cut(s)	330
BsrFI	RCCGGY	1 cut(s)	290
BssAI	RCCGGY	1 cut(s)	290
BssECI	CCNNGG	3 cut(s)	70, 216, 319
BssHII	GCGCGC	1 cut(s)	143
BssMI	GATC	1 cut(s)	55
BssNI	GRCGYC	2 cut(s)	174, 228
Bst2UI	CCWGG	2 cut(s)	266, 320
Bst4CI	ACNGT	3 cut(s)	43, 109, 129
BstACI	GRCGYC	2 cut(s)	174, 228
BstBAI	YACGTR	1 cut(s)	399
BstBI	TTCGAA	1 cut(s)	330
BstC8I	GCNNGC	3 cut(s)	145, 149, 292
BstDEI	CTNAG	2 cut(s)	384, 438
BstF5I	GGATG	1 cut(s)	237
BstFNI	CGCG	7 cut(s)	66, 143, 145, 188, 197, 243, 435
BstHHI	GCGC	6 cut(s)	145, 147, 190, 199, 245, 437
BstKTI	GATC	1 cut(s)	58
BstMAI	GTCTC	1 cut(s)	181
BstMBI	GATC	1 cut(s)	55
BstMWI	GCNNNNNNNGC	9 cut(s)	72, 142, 153, 194, 196, 211, 300, 309, 315
BstNI	CCWGG	2 cut(s)	266, 320
BstSCI	CCNGG	3 cut(s)	264, 276, 318
BstUI	CGCG	7 cut(s)	66, 143, 145, 188, 197, 243, 435
BstV1I	GCAGC	3 cut(s)	296, 306, 407
BstV2I	GAAGAC	1 cut(s)	339
BsuRI	GGCC	2 cut(s)	214, 222
BtsCI	GGATG	1 cut(s)	237
BtsI	GCAGTG	1 cut(s)	456
BtsIMutI	CAGTG	2 cut(s)	125, 456
Cac8I	GCNNGC	3 cut(s)	145, 149, 292
CfoI	GCGC	6 cut(s)	145, 147, 190, 199, 245, 437
Cfr10I	RCCGGY	1 cut(s)	290
Cfr13I	GGNCC	2 cut(s)	213, 220
CseI	GACGC	4 cut(s)	55, 88, 194, 236
Csp6I	GTAC	1 cut(s)	262
CviJI	RGCY	9 cut(s)	22, 151, 214, 222, 290, 403, 420, 442, 449
CviKI_1	RGCY	9 cut(s)	22, 151, 214, 222, 290, 403, 420, 442, 449
CviQI	GTAC	1 cut(s)	262
DdeI	CTNAG	2 cut(s)	384, 438
DpnI	GATC	1 cut(s)	57
DpnII	GATC	1 cut(s)	55
Eco72I	CACGTG	1 cut(s)	399
Eco88I	CYCGRG	1 cut(s)	215
EcoO109I	RGGNCCY	1 cut(s)	220
EcoRII	CCWGG	2 cut(s)	264, 318
Esp3I	CGTCTC	1 cut(s)	181
FaiI	YATR	3 cut(s)	314, 316, 372
FauI	CCCGC	1 cut(s)	202
FauNDI	CATATG	1 cut(s)	314
Fnu4HI	GCNGC	7 cut(s)	200, 203, 231, 295, 310, 367, 421
FokI	GGATG	1 cut(s)	224
Fsp4HI	GCNGC	7 cut(s)	200, 203, 231, 295, 310, 367, 421
GlaI	GCGC	6 cut(s)	144, 146, 189, 198, 244, 436
GluI	GCNGC	7 cut(s)	200, 203, 231, 295, 310, 367, 421
HaeIII	GGCC	2 cut(s)	214, 222
HapII	CCGG	2 cut(s)	277, 291
HgaI	GACGC	4 cut(s)	55, 88, 194, 236
HhaI	GCGC	6 cut(s)	145, 147, 190, 199, 245, 437
Hin1I	GRCGYC	2 cut(s)	174, 228
Hin6I	GCGC	6 cut(s)	143, 145, 188, 197, 243, 435
HinP1I	GCGC	6 cut(s)	143, 145, 188, 197, 243, 435
HinfI	GANTC	2 cut(s)	208, 269
HpaII	CCGG	2 cut(s)	277, 291
HphI	GGTGA	3 cut(s)	31, 97, 358
Hpy166II	GTNNAC	2 cut(s)	34, 394
Hpy188I	TCNGA	3 cut(s)	71, 184, 385
Hpy188III	TCNNGA	1 cut(s)	465
Hpy8I	GTNNAC	2 cut(s)	34, 394
Hpy99I	CGWCG	7 cut(s)	56, 92, 95, 98, 113, 176, 188
HpyAV	CCTTC	3 cut(s)	244, 337, 343
HpyCH4III	ACNGT	3 cut(s)	43, 109, 129
HpyCH4IV	ACGT	3 cut(s)	30, 174, 398
HpyCH4V	TGCA	1 cut(s)	312
HpyF10VI	GCNNNNNNNGC	9 cut(s)	72, 142, 153, 194, 196, 211, 300, 309, 315
HpyF3I	CTNAG	2 cut(s)	384, 438
HpySE526I	ACGT	3 cut(s)	30, 174, 398
Hsp92I	GRCGYC	2 cut(s)	174, 228
HspAI	GCGC	6 cut(s)	143, 145, 188, 197, 243, 435
KroI	GCCGGC	1 cut(s)	290
KroNI	GCCGGC	1 cut(s)	292
Kzo9I	GATC	1 cut(s)	55
LpnPI	CCDG	8 cut(s)	133, 146, 251, 278, 290, 304, 305, 332
Lsp1109I	GCAGC	3 cut(s)	296, 306, 407
MaeII	ACGT	3 cut(s)	30, 174, 398
MaeIII	GTNAC	2 cut(s)	121, 374
MalI	GATC	1 cut(s)	57
MboI	GATC	1 cut(s)	55
MboII	GAAGA	2 cut(s)	94, 344
MmeI	TCCRAC	1 cut(s)	207
MnlI	CCTC	7 cut(s)	16, 65, 125, 128, 176, 190, 211
MroNI	GCCGGC	1 cut(s)	290
MseI	TTAA	1 cut(s)	26
MspA1I	CMGCKG	2 cut(s)	205, 297
MspI	CCGG	2 cut(s)	277, 291
MspR9I	CCNGG	3 cut(s)	266, 278, 320
MvaI	CCWGG	2 cut(s)	266, 320
MvnI	CGCG	7 cut(s)	66, 143, 145, 188, 197, 243, 435
MwoI	GCNNNNNNNGC	9 cut(s)	72, 142, 153, 194, 196, 211, 300, 309, 315
NaeI	GCCGGC	1 cut(s)	292
NciI	CCSGG	1 cut(s)	278
NdeI	CATATG	1 cut(s)	314
NdeII	GATC	1 cut(s)	55
NgoMIV	GCCGGC	1 cut(s)	290
NmuCI	GTSAC	1 cut(s)	121
NspV	TTCGAA	1 cut(s)	330
PauI	GCGCGC	1 cut(s)	143
PcsI	WCGNNNNNNNCGW	6 cut(s)	93, 117, 180, 336, 404, 413
PdiI	GCCGGC	1 cut(s)	292
PfeI	GAWTC	2 cut(s)	208, 269
PkrI	GCNGC	7 cut(s)	201, 204, 232, 296, 311, 368, 422
PmaCI	CACGTG	1 cut(s)	399
PmlI	CACGTG	1 cut(s)	399
Ppu21I	YACGTR	1 cut(s)	399
Psp6I	CCWGG	2 cut(s)	264, 318
PspCI	CACGTG	1 cut(s)	399
PspGI	CCWGG	2 cut(s)	264, 318
PspPI	GGNCC	2 cut(s)	213, 220
PteI	GCGCGC	1 cut(s)	143
RsaI	GTAC	1 cut(s)	263
RsaNI	GTAC	1 cut(s)	262
SaqAI	TTAA	1 cut(s)	26
SatI	GCNGC	7 cut(s)	200, 203, 231, 295, 310, 367, 421
Sau3AI	GATC	1 cut(s)	55
Sau96I	GGNCC	2 cut(s)	213, 220
ScrFI	CCNGG	3 cut(s)	266, 278, 320
SetI	ASST	9 cut(s)	8, 33, 168, 177, 348, 354, 401, 422, 451
SfuI	TTCGAA	1 cut(s)	330
SsiI	CCGC	8 cut(s)	64, 195, 200, 203, 231, 241, 297, 367
StyD4I	CCNGG	3 cut(s)	264, 276, 318
TaaI	ACNGT	3 cut(s)	43, 109, 129
TaiI	ACGT	3 cut(s)	33, 177, 401
TaqI	TCGA	4 cut(s)	272, 330, 407, 416
TauI	GCSGC	4 cut(s)	202, 205, 233, 369
TfiI	GAWTC	2 cut(s)	208, 269
Tru1I	TTAA	1 cut(s)	26
Tru9I	TTAA	1 cut(s)	26
TscAI	CASTG	2 cut(s)	132, 463
TseFI	GTSAC	1 cut(s)	121
TseI	GCWGC	3 cut(s)	294, 309, 420
Tsp45I	GTSAC	1 cut(s)	121
TspDTI	ATGAA	1 cut(s)	226
TspRI	CASTG	2 cut(s)	132, 463
ZraI	GACGTC	1 cut(s)	175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.