AT3G60966

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0019143
Physical Location & Seq
Forward (+)
22552560 .. 22553668
1109 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G60966.1

Sequence Viewer

Length: 420 bp
ATGTTGACTCAATCTGTGTATAGAGTTTGCGAATATATCACCGTTTCTATTTTGATTACCTCCGTCGCCGTCTTTTCTGAAAGATTCGCCACTTTGATCGTCACAAGTCCATGGCGTCGCCACCGCACATTCACGTTCCGTCGGTGGAGAGCGGCGGAAAACGATGATTCGTCTCCACCTTTCTGCGCCGTGTGTCTACAAGAGGCGGAGGAAGGAGAGAAGATGAGGCGATTGACGATTTGCCGTCACTGTTTCCACGCGGATTGCATAGATACATGGCTCAGTGAAATGTCCAAGTGTCCGTTGTGTAGAGCTCAGATTCCTCCGTTACCGCCGGCAAGTCCTATGCTCTTACTGTTTTTTCCGGCGGGTGTGATTGATTTATTCAGCAAGAAAGAATCTCGACGCGTAGCTCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

139

Amino Acids

16.01

Weight (kDa)

8.71

Isoelectric Point (pI)

75.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 62 - 104 7.9e-13 Ring finger domain
zf-rbx1 PF12678 62 - 104 3.1e-09 RING-H2 zinc finger domain
zf-RING_11 PF17123 62 - 90 3.8e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 62 - 103 1.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 62 - 103 3.9e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44578 AT2G44581 AT3G60966
malus_domestica MD01G1155300.v1.1 MD07G1086600.v1.1
prunus_persica Prupe.2G113400_v2.0.a1 Prupe.2G252900_v2.0.a1
pyrus_communis pycom01g17260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 152
AccI GTMKAC 1 cut(s) 196
AccII CGCG 2 cut(s) 260, 408
AciI CCGC 7 cut(s) 124, 152, 155, 206, 260, 332, 368
AcyI GRCGYC 1 cut(s) 115
AflIII ACRYGT 1 cut(s) 406
AluBI AGCT 2 cut(s) 314, 413
AluI AGCT 2 cut(s) 314, 413
Alw21I GWGCWC 1 cut(s) 316
Alw26I GTCTC 1 cut(s) 177
AspLEI GCGC 1 cut(s) 188
AsuHPI GGTGA 1 cut(s) 31
BanII GRGCYC 1 cut(s) 316
Bbv12I GWGCWC 1 cut(s) 316
BceAI ACGGC 3 cut(s) 53, 173, 228
BcoDI GTCTC 1 cut(s) 177
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
BsaHI GRCGYC 1 cut(s) 115
BsaJI CCNNGG 1 cut(s) 110
Bse118I RCCGGY 1 cut(s) 334
BseDI CCNNGG 1 cut(s) 110
BseMII CTCAG 2 cut(s) 295, 329
Bsh1236I CGCG 2 cut(s) 260, 408
BsiHKAI GWGCWC 1 cut(s) 316
BsiSI CCGG 2 cut(s) 335, 365
BsmAI GTCTC 1 cut(s) 177
BsmBI CGTCTC 1 cut(s) 177
Bsp1286I GDGCHC 1 cut(s) 316
Bsp143I GATC 1 cut(s) 96
Bsp19I CCATGG 1 cut(s) 110
BspACI CCGC 7 cut(s) 124, 152, 155, 206, 260, 332, 368
BspCNI CTCAG 2 cut(s) 294, 328
BspFNI CGCG 2 cut(s) 260, 408
BsrBI CCGCTC 1 cut(s) 152
BsrFI RCCGGY 1 cut(s) 334
BssAI RCCGGY 1 cut(s) 334
BssECI CCNNGG 1 cut(s) 110
BssMI GATC 1 cut(s) 96
BssNI GRCGYC 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 110
Bst4CI ACNGT 3 cut(s) 43, 251, 357
BstACI GRCGYC 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 2 cut(s) 281, 315
BstDSI CCRYGG 1 cut(s) 110
BstFNI CGCG 2 cut(s) 260, 408
BstHHI GCGC 1 cut(s) 188
BstKTI GATC 1 cut(s) 99
BstMAI GTCTC 1 cut(s) 177
BstMBI GATC 1 cut(s) 96
BstUI CGCG 2 cut(s) 260, 408
BtgI CCRYGG 1 cut(s) 110
BtsIMutI CAGTG 2 cut(s) 247, 289
Cac8I GCNNGC 1 cut(s) 336
CfoI GCGC 1 cut(s) 188
Cfr10I RCCGGY 1 cut(s) 334
CseI GACGC 2 cut(s) 104, 414
CspCI CAANNNNNGTGG 2 cut(s) 245, 280
CviAII CATG 2 cut(s) 111, 276
CviJI RGCY 3 cut(s) 280, 314, 413
CviKI_1 RGCY 3 cut(s) 280, 314, 413
DdeI CTNAG 2 cut(s) 281, 315
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
EciI GGCGGA 2 cut(s) 170, 221
Ecl136II GAGCTC 1 cut(s) 314
Eco130I CCWWGG 1 cut(s) 110
Eco24I GRGCYC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 314
EcoT14I CCWWGG 1 cut(s) 110
EcoT38I GRGCYC 1 cut(s) 316
ErhI CCWWGG 1 cut(s) 110
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 2 cut(s) 114, 279
FaiI YATR 6 cut(s) 21, 36, 112, 269, 277, 347
FatI CATG 2 cut(s) 110, 275
FauI CCCGC 1 cut(s) 361
FblI GTMKAC 1 cut(s) 196
Fnu4HI GCNGC 1 cut(s) 153
FriOI GRGCYC 1 cut(s) 316
Fsp4HI GCNGC 1 cut(s) 153
GlaI GCGC 1 cut(s) 187
GluI GCNGC 1 cut(s) 153
HapII CCGG 2 cut(s) 335, 365
HgaI GACGC 2 cut(s) 104, 414
HhaI GCGC 1 cut(s) 188
Hin1I GRCGYC 1 cut(s) 115
Hin1II CATG 2 cut(s) 114, 279
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 5 cut(s) 7, 84, 167, 319, 398
HpaII CCGG 2 cut(s) 335, 365
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 2 cut(s) 6, 197
Hpy188I TCNGA 2 cut(s) 79, 318
Hpy188III TCNNGA 1 cut(s) 402
Hpy8I GTNNAC 2 cut(s) 6, 197
Hpy99I CGWCG 4 cut(s) 68, 120, 144, 408
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 3 cut(s) 43, 251, 357
HpyCH4IV ACGT 1 cut(s) 134
HpyCH4V TGCA 1 cut(s) 267
HpyF3I CTNAG 2 cut(s) 281, 315
HpySE526I ACGT 1 cut(s) 134
Hsp92I GRCGYC 1 cut(s) 115
Hsp92II CATG 2 cut(s) 114, 279
HspAI GCGC 1 cut(s) 186
KroI GCCGGC 1 cut(s) 334
KroNI GCCGGC 1 cut(s) 336
Kzo9I GATC 1 cut(s) 96
LpnPI CCDG 2 cut(s) 348, 378
MaeII ACGT 1 cut(s) 134
MaeIII GTNAC 3 cut(s) 100, 245, 327
MalI GATC 1 cut(s) 98
MbiI CCGCTC 1 cut(s) 152
MboI GATC 1 cut(s) 96
MboII GAAGA 1 cut(s) 232
MhlI GDGCHC 1 cut(s) 316
MluI ACGCGT 1 cut(s) 406
MnlI CCTC 5 cut(s) 70, 196, 202, 219, 333
MroNI GCCGGC 1 cut(s) 334
MspI CCGG 2 cut(s) 335, 365
MvnI CGCG 2 cut(s) 260, 408
NaeI GCCGGC 1 cut(s) 336
NcoI CCATGG 1 cut(s) 110
NdeII GATC 1 cut(s) 96
NgoMIV GCCGGC 1 cut(s) 334
NlaIII CATG 2 cut(s) 114, 279
NmuCI GTSAC 2 cut(s) 100, 245
PdiI GCCGGC 1 cut(s) 336
PfeI GAWTC 4 cut(s) 84, 167, 319, 398
PkrI GCNGC 1 cut(s) 154
Psp124BI GAGCTC 1 cut(s) 316
SacI GAGCTC 1 cut(s) 316
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 1 cut(s) 96
SduI GDGCHC 1 cut(s) 316
SetI ASST 5 cut(s) 62, 137, 181, 316, 415
SsiI CCGC 7 cut(s) 124, 152, 155, 206, 260, 332, 368
SstI GAGCTC 1 cut(s) 316
StyI CCWWGG 1 cut(s) 110
TaaI ACNGT 3 cut(s) 43, 251, 357
TaiI ACGT 1 cut(s) 137
TaqI TCGA 1 cut(s) 403
TauI GCSGC 1 cut(s) 155
TfiI GAWTC 4 cut(s) 84, 167, 319, 398
TscAI CASTG 2 cut(s) 254, 289
TseFI GTSAC 2 cut(s) 100, 245
Tsp45I GTSAC 2 cut(s) 100, 245
TspGWI ACGGA 4 cut(s) 52, 128, 291, 315
TspRI CASTG 2 cut(s) 254, 289
XmiI GTMKAC 1 cut(s) 196
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.