AT2G44581

ubiquitin-protein transferase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0019143
Physical Location & Seq
Reverse (-)
18397874 .. 18398504
631 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44581.1

Sequence Viewer

Length: 438 bp
ATGTTTGTTGAAACTGTGATTCAACTCTCTCAGTTCATTGCCGAATCCGTCATTATCGTCTCTGTAGCCGTCTTCACTCTCCGTCTCTTCTATAGATTCGCTTGTTTCCTCGCGAGCCGTTCTTGGCGTCGTTACCGTACATTCACGGTCCATCATCGCCGGAGATGGAGAAAAACCACAGCCGAGGAAAAACATTCATCACCGTATTGTACGATTTGTCTCGAAAATGCGACGGAGGGAGAAAAGATGAGGCGGATAGCAGCATGCAGTCACTGTTTCCACGTCGATTGCATTGATCCGTGGTTGGAGAAGAAGTCCATGTGTCCTTTGTGTAGAGCTGAGATCCCGCCGGTCCCGCCGGGAAATCCTCTGCGCGCGCTTTTTATTCCTCCCGGCGTGATTGAGATGTTCACCAAAGGAATCGACTCCGACGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

145

Amino Acids

16.78

Weight (kDa)

9.03

Isoelectric Point (pI)

68.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_2 PF13923 69 - 111 1.4e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 70 - 112 3.2e-13 Ring finger domain
zf-RING_11 PF17123 70 - 98 2.2e-09 RING-like zinc finger
zf-C3HC4 PF00097 70 - 111 9.5e-06 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 71 - 112 5.3e-10 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019143)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44578 AT2G44581 AT3G60966
malus_domestica MD01G1155300.v1.1 MD07G1086600.v1.1
prunus_persica Prupe.2G113400_v2.0.a1 Prupe.2G252900_v2.0.a1
pyrus_communis pycom01g17260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 113, 375, 377, 434
AciI CCGC 3 cut(s) 253, 347, 356
AclWI GGATC 2 cut(s) 290, 337
AcyI GRCGYC 1 cut(s) 127
AfaI GTAC 2 cut(s) 139, 211
AflIII ACRYGT 1 cut(s) 432
AgsI TTSAA 2 cut(s) 11, 23
AjiI CACGTC 1 cut(s) 283
AluBI AGCT 1 cut(s) 338
AluI AGCT 1 cut(s) 338
Alw26I GTCTC 3 cut(s) 64, 89, 224
AlwI GGATC 2 cut(s) 290, 337
AlwNI CAGNNNCTG 1 cut(s) 273
ApeKI GCWGC 1 cut(s) 260
AspLEI GCGC 3 cut(s) 375, 377, 379
AspS9I GGNCC 2 cut(s) 148, 352
AsuC2I CCSGG 2 cut(s) 360, 393
AsuHPI GGTGA 2 cut(s) 192, 403
AvaII GGWCC 2 cut(s) 148, 352
BbsI GAAGAC 1 cut(s) 64
BbvI GCAGC 1 cut(s) 272
BccI CCATC 2 cut(s) 159, 159
BceAI ACGGC 2 cut(s) 53, 102
BcnI CCSGG 2 cut(s) 360, 393
BcoDI GTCTC 3 cut(s) 64, 89, 224
BfmI CTRYAG 2 cut(s) 63, 91
BisI GCNGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 262
Bme1390I CCNGG 2 cut(s) 360, 393
Bme18I GGWCC 2 cut(s) 148, 352
BmgBI CACGTC 1 cut(s) 283
BmgT120I GGNCC 2 cut(s) 148, 352
BmiI GGNNCC 1 cut(s) 354
BmrFI CCNGG 2 cut(s) 360, 393
BpiI GAAGAC 1 cut(s) 64
BpuMI CCSGG 2 cut(s) 360, 393
BsaHI GRCGYC 1 cut(s) 127
BsaJI CCNNGG 2 cut(s) 183, 299
BsaXI ACNNNNNCTCC 2 cut(s) 299, 329
Bse118I RCCGGY 1 cut(s) 349
Bse3DI GCAATG 1 cut(s) 36
BseDI CCNNGG 2 cut(s) 183, 299
BseMI GCAATG 1 cut(s) 36
BseMII CTCAG 2 cut(s) 44, 330
BsePI GCGCGC 2 cut(s) 373, 375
BseXI GCAGC 1 cut(s) 272
Bsh1236I CGCG 4 cut(s) 113, 375, 377, 434
BsiSI CCGG 4 cut(s) 160, 350, 359, 393
BslFI GGGAC 1 cut(s) 338
BsmAI GTCTC 3 cut(s) 64, 89, 224
BsmBI CGTCTC 2 cut(s) 64, 89
BsmFI GGGAC 1 cut(s) 338
Bsp143I GATC 2 cut(s) 295, 342
Bsp68I TCGCGA 1 cut(s) 113
BspACI CCGC 3 cut(s) 253, 347, 356
BspCNI CTCAG 2 cut(s) 43, 331
BspFNI CGCG 4 cut(s) 113, 375, 377, 434
BspLI GGNNCC 1 cut(s) 354
BspPI GGATC 2 cut(s) 290, 337
BsrDI GCAATG 1 cut(s) 36
BsrFI RCCGGY 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 183, 299
BssHII GCGCGC 2 cut(s) 373, 375
BssMI GATC 2 cut(s) 295, 342
BssNI GRCGYC 1 cut(s) 127
Bst4CI ACNGT 5 cut(s) 16, 137, 148, 204, 275
Bst6I CTCTTC 1 cut(s) 92
BstACI GRCGYC 1 cut(s) 127
BstC8I GCNNGC 4 cut(s) 115, 265, 375, 377
BstDEI CTNAG 2 cut(s) 30, 339
BstDSI CCRYGG 1 cut(s) 299
BstFNI CGCG 4 cut(s) 113, 375, 377, 434
BstHHI GCGC 3 cut(s) 375, 377, 379
BstKTI GATC 2 cut(s) 298, 345
BstMAI GTCTC 3 cut(s) 64, 89, 224
BstMBI GATC 2 cut(s) 295, 342
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstNSI RCATGY 1 cut(s) 267
BstSCI CCNGG 2 cut(s) 358, 391
BstSFI CTRYAG 2 cut(s) 63, 91
BstUI CGCG 4 cut(s) 113, 375, 377, 434
BstV1I GCAGC 1 cut(s) 272
BstV2I GAAGAC 1 cut(s) 64
BstX2I RGATCY 1 cut(s) 342
BstYI RGATCY 1 cut(s) 342
BtgI CCRYGG 1 cut(s) 299
BtgZI GCGATG 1 cut(s) 140
BtrI CACGTC 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 271
BtuMI TCGCGA 1 cut(s) 113
Cac8I GCNNGC 4 cut(s) 115, 265, 375, 377
CaiI CAGNNNCTG 1 cut(s) 273
CfoI GCGC 3 cut(s) 375, 377, 379
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 148, 352
CseI GACGC 1 cut(s) 116
Csp6I GTAC 2 cut(s) 138, 210
CspCI CAANNNNNGTGG 2 cut(s) 269, 304
CviAII CATG 2 cut(s) 264, 319
CviJI RGCY 4 cut(s) 68, 117, 182, 338
CviKI_1 RGCY 4 cut(s) 68, 117, 182, 338
CviQI GTAC 2 cut(s) 138, 210
DdeI CTNAG 2 cut(s) 30, 339
DpnI GATC 2 cut(s) 297, 344
DpnII GATC 2 cut(s) 295, 342
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
EciI GGCGGA 1 cut(s) 268
Eco47I GGWCC 2 cut(s) 148, 352
Esp3I CGTCTC 2 cut(s) 64, 89
FaeI CATG 2 cut(s) 267, 322
FaiI YATR 3 cut(s) 93, 265, 320
FaqI GGGAC 1 cut(s) 338
FatI CATG 2 cut(s) 263, 318
FauI CCCGC 2 cut(s) 354, 363
Fnu4HI GCNGC 1 cut(s) 261
Fsp4HI GCNGC 1 cut(s) 261
GlaI GCGC 3 cut(s) 374, 376, 378
GluI GCNGC 1 cut(s) 261
HapII CCGG 4 cut(s) 160, 350, 359, 393
HgaI GACGC 1 cut(s) 116
HhaI GCGC 3 cut(s) 375, 377, 379
Hin1I GRCGYC 1 cut(s) 127
Hin1II CATG 2 cut(s) 267, 322
Hin6I GCGC 3 cut(s) 373, 375, 377
HinP1I GCGC 3 cut(s) 373, 375, 377
HinfI GANTC 5 cut(s) 19, 44, 96, 420, 425
HpaII CCGG 4 cut(s) 160, 350, 359, 393
HphI GGTGA 2 cut(s) 192, 403
Hpy166II GTNNAC 1 cut(s) 411
Hpy188I TCNGA 1 cut(s) 430
Hpy188III TCNNGA 2 cut(s) 112, 221
Hpy8I GTNNAC 1 cut(s) 411
Hpy99I CGWCG 4 cut(s) 132, 235, 287, 434
HpyCH4III ACNGT 5 cut(s) 16, 137, 148, 204, 275
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 2 cut(s) 267, 291
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpyF3I CTNAG 2 cut(s) 30, 339
HpySE526I ACGT 1 cut(s) 282
Hsp92I GRCGYC 1 cut(s) 127
Hsp92II CATG 2 cut(s) 267, 322
HspAI GCGC 3 cut(s) 373, 375, 377
Kzo9I GATC 2 cut(s) 295, 342
LpnPI CCDG 4 cut(s) 173, 363, 372, 406
Lsp1109I GCAGC 1 cut(s) 272
MaeII ACGT 1 cut(s) 282
MaeIII GTNAC 2 cut(s) 131, 269
MalI GATC 2 cut(s) 297, 344
MboI GATC 2 cut(s) 295, 342
MboII GAAGA 3 cut(s) 64, 79, 322
MflI RGATCY 1 cut(s) 342
MluI ACGCGT 1 cut(s) 432
MlyI GAGTC 1 cut(s) 419
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 6 cut(s) 119, 178, 229, 243, 378, 399
MspI CCGG 4 cut(s) 160, 350, 359, 393
MspR9I CCNGG 2 cut(s) 360, 393
MvnI CGCG 4 cut(s) 113, 375, 377, 434
MwoI GCNNNNNNNGC 1 cut(s) 355
NciI CCSGG 2 cut(s) 360, 393
NdeII GATC 2 cut(s) 295, 342
NlaIII CATG 2 cut(s) 267, 322
NlaIV GGNNCC 1 cut(s) 354
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 1 cut(s) 269
NruI TCGCGA 1 cut(s) 113
NspI RCATGY 1 cut(s) 267
PaeI GCATGC 1 cut(s) 267
PauI GCGCGC 2 cut(s) 373, 375
PfeI GAWTC 4 cut(s) 19, 44, 96, 420
PkrI GCNGC 1 cut(s) 262
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PspN4I GGNNCC 1 cut(s) 354
PspPI GGNCC 2 cut(s) 148, 352
PstNI CAGNNNCTG 1 cut(s) 273
PsuI RGATCY 1 cut(s) 342
PteI GCGCGC 2 cut(s) 373, 375
RruI TCGCGA 1 cut(s) 113
RsaI GTAC 2 cut(s) 139, 211
RsaNI GTAC 2 cut(s) 138, 210
SatI GCNGC 1 cut(s) 261
Sau3AI GATC 2 cut(s) 295, 342
Sau96I GGNCC 2 cut(s) 148, 352
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 2 cut(s) 360, 393
SetI ASST 2 cut(s) 285, 340
SfcI CTRYAG 2 cut(s) 63, 91
SinI GGWCC 2 cut(s) 148, 352
SphI GCATGC 1 cut(s) 267
SsiI CCGC 3 cut(s) 253, 347, 356
StyD4I CCNGG 2 cut(s) 358, 391
TaaI ACNGT 5 cut(s) 16, 137, 148, 204, 275
TaiI ACGT 1 cut(s) 285
TaqI TCGA 3 cut(s) 222, 285, 423
TfiI GAWTC 4 cut(s) 19, 44, 96, 420
TscAI CASTG 1 cut(s) 278
TseFI GTSAC 1 cut(s) 269
TseI GCWGC 1 cut(s) 260
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 2 cut(s) 25, 186
TspGWI ACGGA 4 cut(s) 37, 71, 248, 288
TspRI CASTG 1 cut(s) 278
VpaK11BI GGWCC 2 cut(s) 148, 352
XceI RCATGY 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.