AT2G46600

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0016575
Physical Location & Seq
Forward (+)
19136019 .. 19136600
582 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46600.1

Sequence Viewer

Length: 408 bp
ATGGAACCAACCGAGAAATCTATGTTACTAGAAACTACTAGTACCACGAAGATGGAGACCAAATACGAAGATATGTTACCAGTTATGGCGGAGAAGATGGATGTTGAAGAGTTTGTATCAGAGTTATGCAAAGGTTTCAGTTTGCTTGCGGATCCAGAGAGACATCTCATCACAGCTGAGTCTCTAAGACGAAACTCAGGGATACTTGGAATTGAAGGTATGAGCAAGGAAGATGCTCAAGGAATGGTTAGAGAAGGAGACCTCGATGGAGATGGTGCTCTTAACCAAACCGAATTCTGCGTTCTCATGGTTCGGTTAAGCCCTGAGATGATGGAAGACGCCGAAACTTGGTTGGAGAAAGCACTCACCCAAGAACTATGTAATCACAATCTCTCTTCTATGCCTTGA

Protein Analysis

135

Amino Acids

15.17

Weight (kDa)

4.34

Isoelectric Point (pI)

56.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 57 - 105 5.1e-16 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016575)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46600
fragaria_vesca FvH4_7g17080
malus_domestica MD01G1085500.v1.1 MD07G1154400.v1.1
prunus_persica Prupe.2G193100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0360491
rosa_laevigata RLG00000027803
rosa_multiflora Rmu_ssc0000337.1_g000010
rosa_roxburghii Rroxscaffold_4G00296050
rosa_rugosa Rorug01G0278700
rosa_samantha Rh1AG291400 Rh1BG256400 Rh1DG286200
rosa_wichuraiana Rw1G025810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 89, 149
AclWI GGATC 2 cut(s) 146, 159
AcsI RAATTY 1 cut(s) 293
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 1 cut(s) 43
AfiI CCNNNNNNNGG 1 cut(s) 348
AgsI TTSAA 2 cut(s) 107, 215
AhlI ACTAGT 1 cut(s) 38
AluBI AGCT 1 cut(s) 176
AluI AGCT 1 cut(s) 176
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 4 cut(s) 50, 154, 186, 252
AlwI GGATC 2 cut(s) 146, 159
ApoI RAATTY 1 cut(s) 293
AsuHPI GGTGA 1 cut(s) 358
BamHI GGATCC 1 cut(s) 151
BarI GAAGNNNNNNTAC 2 cut(s) 60, 92
BbsI GAAGAC 1 cut(s) 342
Bbv12I GWGCWC 1 cut(s) 280
BccI CCATC 5 cut(s) 46, 91, 260, 266, 325
BciVI GTATCC 1 cut(s) 195
BcoDI GTCTC 4 cut(s) 50, 154, 186, 252
BcuI ACTAGT 1 cut(s) 38
BfaI CTAG 2 cut(s) 29, 39
BfuI GTATCC 1 cut(s) 195
BmiI GGNNCC 2 cut(s) 6, 153
BmsI GCATC 1 cut(s) 223
BpiI GAAGAC 1 cut(s) 342
BpuEI CTTGAG 1 cut(s) 222
BsaHI GRCGYC 1 cut(s) 339
BsaI GGTCTC 2 cut(s) 50, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 348
Bse1I ACTGG 1 cut(s) 80
BseGI GGATG 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 348
BseMII CTCAG 3 cut(s) 168, 210, 315
BseNI ACTGG 1 cut(s) 80
BsiHKAI GWGCWC 1 cut(s) 280
BslI CCNNNNNNNGG 1 cut(s) 348
BsmAI GTCTC 4 cut(s) 50, 154, 186, 252
Bso31I GGTCTC 2 cut(s) 50, 252
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 1 cut(s) 151
BspACI CCGC 2 cut(s) 89, 149
BspCNI CTCAG 3 cut(s) 169, 209, 316
BspLI GGNNCC 2 cut(s) 6, 153
BspPI GGATC 2 cut(s) 146, 159
BspTNI GGTCTC 2 cut(s) 50, 252
BsrI ACTGG 1 cut(s) 80
BssMI GATC 1 cut(s) 151
BssNI GRCGYC 1 cut(s) 339
Bst6I CTCTTC 2 cut(s) 102, 400
BstACI GRCGYC 1 cut(s) 339
BstC8I GCNNGC 1 cut(s) 147
BstDEI CTNAG 4 cut(s) 177, 185, 196, 324
BstF5I GGATG 1 cut(s) 106
BstKTI GATC 1 cut(s) 154
BstMAI GTCTC 4 cut(s) 50, 154, 186, 252
BstMBI GATC 1 cut(s) 151
BstV2I GAAGAC 1 cut(s) 342
BstX2I RGATCY 1 cut(s) 151
BstXI CCANNNNNNTGG 1 cut(s) 52
BstYI RGATCY 1 cut(s) 151
BsuI GTATCC 1 cut(s) 195
BtsCI GGATG 1 cut(s) 106
Cac8I GCNNGC 1 cut(s) 147
CseI GACGC 1 cut(s) 347
Csp6I GTAC 1 cut(s) 42
CviAII CATG 1 cut(s) 307
CviJI RGCY 2 cut(s) 176, 321
CviKI_1 RGCY 2 cut(s) 176, 321
CviQI GTAC 1 cut(s) 42
DdeI CTNAG 4 cut(s) 177, 185, 196, 324
DpnI GATC 1 cut(s) 153
DpnII GATC 1 cut(s) 151
Eam1104I CTCTTC 2 cut(s) 102, 400
EarI CTCTTC 2 cut(s) 102, 400
EciI GGCGGA 1 cut(s) 104
Eco31I GGTCTC 2 cut(s) 50, 252
EcoRI GAATTC 1 cut(s) 293
FaeI CATG 1 cut(s) 310
FaiI YATR 8 cut(s) 23, 74, 86, 127, 221, 308, 379, 401
FatI CATG 1 cut(s) 306
FokI GGATG 1 cut(s) 113
FspBI CTAG 2 cut(s) 29, 39
HgaI GACGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 1 cut(s) 310
HinfI GANTC 1 cut(s) 179
HphI GGTGA 1 cut(s) 358
Hpy188I TCNGA 1 cut(s) 121
Hpy188III TCNNGA 1 cut(s) 155
HpyAV CCTTC 2 cut(s) 209, 248
HpyCH4V TGCA 1 cut(s) 129
HpyF3I CTNAG 4 cut(s) 177, 185, 196, 324
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 1 cut(s) 310
Kzo9I GATC 1 cut(s) 151
LpnPI CCDG 4 cut(s) 93, 168, 183, 336
LweI GCATC 1 cut(s) 223
MaeI CTAG 2 cut(s) 29, 39
MaeIII GTNAC 2 cut(s) 24, 75
MalI GATC 1 cut(s) 153
MboI GATC 1 cut(s) 151
MboII GAAGA 7 cut(s) 61, 80, 106, 119, 242, 347, 387
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 280
MluCI AATT 2 cut(s) 210, 293
MlyI GAGTC 1 cut(s) 188
MmeI TCCRAC 1 cut(s) 333
MnlI CCTC 1 cut(s) 272
MseI TTAA 2 cut(s) 282, 317
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 1 cut(s) 176
NdeII GATC 1 cut(s) 151
NlaIII CATG 1 cut(s) 310
NlaIV GGNNCC 2 cut(s) 6, 153
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
PspN4I GGNNCC 2 cut(s) 6, 153
PsuI RGATCY 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 176
RsaI GTAC 1 cut(s) 43
RsaNI GTAC 1 cut(s) 42
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 2 cut(s) 282, 317
Sau3AI GATC 1 cut(s) 151
SchI GAGTC 1 cut(s) 188
SduI GDGCHC 1 cut(s) 280
SetI ASST 4 cut(s) 136, 178, 220, 264
SfaNI GCATC 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 237
SmoI CTYRAG 1 cut(s) 237
SpeI ACTAGT 1 cut(s) 38
Sse9I AATT 2 cut(s) 210, 293
SsiI CCGC 2 cut(s) 89, 149
SspMI CTAG 2 cut(s) 29, 39
TaqI TCGA 1 cut(s) 264
TasI AATT 2 cut(s) 210, 293
Tru1I TTAA 2 cut(s) 282, 317
Tru9I TTAA 2 cut(s) 282, 317
XapI RAATTY 1 cut(s) 293
XspI CTAG 2 cut(s) 29, 39
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.