AT2G47780

REF SRPP-like protein At2g47780

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0012392
Physical Location & Seq
Forward (+)
19570040 .. 19571034
995 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G47780.1

Sequence Viewer

Length: 708 bp
ATGGCTGAAGATGAAATAGTAGTCGAAGAAGAGCAATCACAGCCACAGGAGATTACTCCAGTTCCACCATCTTCTTCGTCTTCGCCTTCGTTAGTGGTGGAAGACGACGATGAGATGAAGCTCAAGCACTTGGAATTCATTCAAGTCGCGGCGGTTTACTTCGCCGCCTGTTTCTCGACCCTCTACGAGTTGGCTAAAGACAACGCTGGTCCACTCAAACTCGGCGTCGAGAACATTGAAGATTGCGTTCGAACCGTGCTTGCTCCATTATACGAAAAATTCCACGACGTTCCTTTCAAGCTTCTCCTTTTCGTCGATCGTAAGGTGGACGATGTGTTCTTCGATGTTGAGACATATGTTCCGTCATTGGTGAAGCAAGCTTCTAGCCAAGCGCTCACGGTGGCCACGGAGGTGCAGCGCACCGGCGTTGTGGACACTACCAAGAGCATTGCAAGAAGCGTCCGGGACAAGTACGAGCCAGCGGCAGAGTATTACGCAGCGACGCTGTGGCGTTTACTGAATCAACTGCCGTTGTTCCCTGAAGTTGCGCATTTAGTGATCCCCACGGCGTTTTACTGGTCAGAGAAGTACAACGATGCGGTTCGTTACGTTGGCGACAGAGACTACTTTGGGGCAGAGTATCTACCCATGATTCCTATTGAGAAGATCTCTGATATTTTGGAACAAGATCAGTGTCGAGCTGATTGA

Protein Analysis

235

Amino Acids

26.64

Weight (kDa)

4.44

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 36 - 228 4.3e-79 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G47780
fragaria_vesca FvH4_2g25450
malus_domestica MD03G1285600.v1.1 MD11G1305000.v1.1
prunus_persica Prupe.8G258700_v2.0.a1 Prupe.8G258700_v2.0.a1
pyrus_communis pycom11g26680
rosa_chinensis RchiOBHm_Chr6g0293681
rosa_laevigata RLG00000011933
rosa_multiflora Rmu_co8092110.1_g000001 Rmu_sc0008436.1_g000002 Rmu_sc0028689.1_g000001
rosa_roxburghii Rroxscaffold_7G00173990
rosa_rugosa Rorug06G0237400
rosa_samantha Rh6AG350000 Rh6BG357500 Rh6CG364300 Rh6DG351200
rosa_wichuraiana Rw6G030470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 549
AccII CGCG 1 cut(s) 149
AciI CCGC 5 cut(s) 149, 152, 165, 482, 599
AclWI GGATC 1 cut(s) 553
AcoI YGGCCR 1 cut(s) 402
AcsI RAATTY 2 cut(s) 134, 278
AcuI CTGAAG 2 cut(s) 27, 561
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 2 cut(s) 473, 590
AfeI AGCGCT 1 cut(s) 393
AgsI TTSAA 3 cut(s) 143, 239, 298
AleI CACNNNNGTG 1 cut(s) 410
AloI GAACNNNNNNTCC 2 cut(s) 320, 352
AluBI AGCT 4 cut(s) 121, 301, 380, 701
AluI AGCT 4 cut(s) 121, 301, 380, 701
Alw26I GTCTC 2 cut(s) 344, 615
AlwI GGATC 1 cut(s) 553
Aor51HI AGCGCT 1 cut(s) 393
AoxI GGCC 1 cut(s) 402
ApeKI GCWGC 2 cut(s) 415, 497
ApoI RAATTY 2 cut(s) 134, 278
ArsI GACNNNNNNTTYG 2 cut(s) 210, 242
Asp700I GAANNNNTTC 1 cut(s) 138
AspLEI GCGC 3 cut(s) 394, 420, 550
AspS9I GGNCC 1 cut(s) 209
AsuC2I CCSGG 1 cut(s) 464
AsuHPI GGTGA 1 cut(s) 382
AsuII TTCGAA 1 cut(s) 250
AvaII GGWCC 1 cut(s) 209
BalI TGGCCA 1 cut(s) 404
BbsI GAAGAC 2 cut(s) 72, 108
BbvI GCAGC 2 cut(s) 427, 509
BccI CCATC 1 cut(s) 76
BceAI ACGGC 2 cut(s) 514, 582
BcnI CCSGG 1 cut(s) 464
BcoDI GTCTC 2 cut(s) 344, 615
BfaI CTAG 1 cut(s) 384
BfoI RGCGCY 1 cut(s) 395
BglII AGATCT 1 cut(s) 666
BisI GCNGC 5 cut(s) 150, 165, 416, 483, 498
BlsI GCNGC 5 cut(s) 151, 166, 417, 484, 499
Bme1390I CCNGG 1 cut(s) 464
Bme18I GGWCC 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 209
BmrFI CCNGG 1 cut(s) 464
BmsI GCATC 1 cut(s) 586
BpiI GAAGAC 2 cut(s) 72, 108
BplI GAGNNNNNCTC 2 cut(s) 653, 685
BpmI CTGGAG 1 cut(s) 42
Bpu14I TTCGAA 1 cut(s) 250
BpuEI CTTGAG 1 cut(s) 107
BpuMI CCSGG 1 cut(s) 464
BsaHI GRCGYC 1 cut(s) 225
BsaJI CCNNGG 2 cut(s) 405, 564
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 2 cut(s) 59, 581
Bse3DI GCAATG 1 cut(s) 447
BseDI CCNNGG 2 cut(s) 405, 564
BseMI GCAATG 1 cut(s) 447
BseNI ACTGG 2 cut(s) 59, 581
BseXI GCAGC 2 cut(s) 427, 509
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 1 cut(s) 404
BsiEI CGRYCG 1 cut(s) 319
BsiSI CCGG 2 cut(s) 423, 463
BslFI GGGAC 1 cut(s) 479
BsmAI GTCTC 2 cut(s) 344, 615
BsmFI GGGAC 1 cut(s) 479
BsnI GGCC 1 cut(s) 404
Bsp119I TTCGAA 1 cut(s) 250
Bsp143I GATC 4 cut(s) 316, 558, 666, 688
BspACI CCGC 5 cut(s) 149, 152, 165, 482, 599
BspANI GGCC 1 cut(s) 404
BspFNI CGCG 1 cut(s) 149
BspPI GGATC 1 cut(s) 553
BspQI GCTCTTC 1 cut(s) 24
BspT104I TTCGAA 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 447
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 2 cut(s) 59, 581
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 2 cut(s) 405, 564
BssMI GATC 4 cut(s) 316, 558, 666, 688
BssNI GRCGYC 1 cut(s) 225
Bst4CI ACNGT 2 cut(s) 256, 400
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 1 cut(s) 225
BstBI TTCGAA 1 cut(s) 250
BstC8I GCNNGC 3 cut(s) 261, 378, 480
BstDSI CCRYGG 2 cut(s) 405, 564
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 1 cut(s) 395
BstHHI GCGC 3 cut(s) 394, 420, 550
BstKTI GATC 4 cut(s) 319, 561, 669, 691
BstMAI GTCTC 2 cut(s) 344, 615
BstMBI GATC 4 cut(s) 316, 558, 666, 688
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 1 cut(s) 40
BstSCI CCNGG 1 cut(s) 462
BstUI CGCG 1 cut(s) 149
BstV1I GCAGC 2 cut(s) 427, 509
BstV2I GAAGAC 2 cut(s) 72, 108
BstX2I RGATCY 1 cut(s) 666
BstYI RGATCY 1 cut(s) 666
BsuRI GGCC 1 cut(s) 404
BtgI CCRYGG 2 cut(s) 405, 564
BtsIMutI CAGTG 1 cut(s) 698
Cac8I GCNNGC 3 cut(s) 261, 378, 480
CfoI GCGC 3 cut(s) 394, 420, 550
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 1 cut(s) 209
CseI GACGC 3 cut(s) 214, 448, 511
Csp6I GTAC 2 cut(s) 472, 589
CviAII CATG 1 cut(s) 649
CviQI GTAC 2 cut(s) 472, 589
DpnI GATC 4 cut(s) 318, 560, 668, 690
DpnII GATC 4 cut(s) 316, 558, 666, 688
EaeI YGGCCR 1 cut(s) 402
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
Eco47I GGWCC 1 cut(s) 209
Eco47III AGCGCT 1 cut(s) 393
Eco57I CTGAAG 2 cut(s) 27, 561
EcoRI GAATTC 1 cut(s) 134
FaeI CATG 1 cut(s) 652
FaiI YATR 4 cut(s) 271, 355, 357, 650
FaqI GGGAC 1 cut(s) 479
FatI CATG 1 cut(s) 648
FauNDI CATATG 1 cut(s) 355
Fnu4HI GCNGC 5 cut(s) 150, 165, 416, 483, 498
Fsp4HI GCNGC 5 cut(s) 150, 165, 416, 483, 498
FspBI CTAG 1 cut(s) 384
FspI TGCGCA 1 cut(s) 549
GlaI GCGC 3 cut(s) 393, 419, 549
GluI GCNGC 5 cut(s) 150, 165, 416, 483, 498
GsuI CTGGAG 1 cut(s) 42
HaeII RGCGCY 1 cut(s) 395
HaeIII GGCC 1 cut(s) 404
HapII CCGG 2 cut(s) 423, 463
HgaI GACGC 3 cut(s) 214, 448, 511
HhaI GCGC 3 cut(s) 394, 420, 550
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 1 cut(s) 652
Hin6I GCGC 3 cut(s) 392, 418, 548
HinP1I GCGC 3 cut(s) 392, 418, 548
HindIII AAGCTT 2 cut(s) 299, 378
HinfI GANTC 2 cut(s) 520, 652
HpaII CCGG 2 cut(s) 423, 463
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 5 cut(s) 157, 212, 328, 433, 515
Hpy188I TCNGA 2 cut(s) 583, 673
Hpy188III TCNNGA 2 cut(s) 175, 229
Hpy8I GTNNAC 5 cut(s) 157, 212, 328, 433, 515
Hpy99I CGWCG 5 cut(s) 110, 230, 290, 317, 505
HpyAV CCTTC 1 cut(s) 96
HpyCH4III ACNGT 2 cut(s) 256, 400
HpyCH4IV ACGT 2 cut(s) 288, 609
HpyCH4V TGCA 2 cut(s) 415, 452
HpyF10VI GCNNNNNNNGC 1 cut(s) 40
HpySE526I ACGT 2 cut(s) 288, 609
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 1 cut(s) 652
HspAI GCGC 3 cut(s) 392, 418, 548
Kzo9I GATC 4 cut(s) 316, 558, 666, 688
LguI GCTCTTC 1 cut(s) 24
LmnI GCTCC 1 cut(s) 268
LpnPI CCDG 9 cut(s) 32, 72, 181, 192, 436, 476, 492, 552, 562
Lsp1109I GCAGC 2 cut(s) 427, 509
LweI GCATC 1 cut(s) 586
MaeI CTAG 1 cut(s) 384
MaeII ACGT 2 cut(s) 288, 609
MaeIII GTNAC 1 cut(s) 605
MalI GATC 4 cut(s) 318, 560, 668, 690
MboI GATC 4 cut(s) 316, 558, 666, 688
MflI RGATCY 1 cut(s) 666
MlsI TGGCCA 1 cut(s) 404
MluCI AATT 2 cut(s) 134, 278
MluNI TGGCCA 1 cut(s) 404
MnlI CCTC 2 cut(s) 191, 403
Mox20I TGGCCA 1 cut(s) 404
MroXI GAANNNNTTC 1 cut(s) 138
MscI TGGCCA 1 cut(s) 404
MslI CAYNNNNRTG 1 cut(s) 410
Msp20I TGGCCA 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 482
MspI CCGG 2 cut(s) 423, 463
MspR9I CCNGG 1 cut(s) 464
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 1 cut(s) 40
NciI CCSGG 1 cut(s) 464
NdeI CATATG 1 cut(s) 355
NdeII GATC 4 cut(s) 316, 558, 666, 688
NlaIII CATG 1 cut(s) 652
NmeAIII GCCGAG 1 cut(s) 201
NsbI TGCGCA 1 cut(s) 549
NspV TTCGAA 1 cut(s) 250
OliI CACNNNNGTG 1 cut(s) 410
PciSI GCTCTTC 1 cut(s) 24
PdmI GAANNNNTTC 1 cut(s) 138
PfeI GAWTC 2 cut(s) 520, 652
PfoI TCCNGGA 1 cut(s) 462
PkrI GCNGC 5 cut(s) 151, 166, 417, 484, 499
Ple19I CGATCG 1 cut(s) 319
PspPI GGNCC 1 cut(s) 209
PsuI RGATCY 1 cut(s) 666
PvuI CGATCG 1 cut(s) 319
RsaI GTAC 2 cut(s) 473, 590
RsaNI GTAC 2 cut(s) 472, 589
RseI CAYNNNNRTG 1 cut(s) 410
SapI GCTCTTC 1 cut(s) 24
SatI GCNGC 5 cut(s) 150, 165, 416, 483, 498
Sau3AI GATC 4 cut(s) 316, 558, 666, 688
Sau96I GGNCC 1 cut(s) 209
ScrFI CCNGG 1 cut(s) 464
SetI ASST 8 cut(s) 123, 291, 303, 327, 382, 414, 612, 703
SfaNI GCATC 1 cut(s) 586
SfuI TTCGAA 1 cut(s) 250
SgrAI CRCCGGYG 1 cut(s) 422
SinI GGWCC 1 cut(s) 209
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 122
SmoI CTYRAG 1 cut(s) 122
Sse9I AATT 2 cut(s) 134, 278
SsiI CCGC 5 cut(s) 149, 152, 165, 482, 599
SspMI CTAG 1 cut(s) 384
StyD4I CCNGG 1 cut(s) 462
TaaI ACNGT 2 cut(s) 256, 400
TaiI ACGT 2 cut(s) 291, 612
TaqI TCGA 7 cut(s) 24, 176, 228, 250, 315, 342, 697
TasI AATT 2 cut(s) 134, 278
TatI WGTACW 1 cut(s) 588
TauI GCSGC 3 cut(s) 152, 167, 485
TfiI GAWTC 2 cut(s) 520, 652
TscAI CASTG 1 cut(s) 698
TseI GCWGC 2 cut(s) 415, 497
TspDTI ATGAA 3 cut(s) 27, 127, 131
TspGWI ACGGA 2 cut(s) 351, 422
TspRI CASTG 1 cut(s) 698
VpaK11BI GGWCC 1 cut(s) 209
XapI RAATTY 2 cut(s) 134, 278
XmnI GAANNNNTTC 1 cut(s) 138
XspI CTAG 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.